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<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q977Z7</accession>
    <name>Q977Z7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator PadR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1573729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917749.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q977Z7"/>
    <dbReference type="STRING" id="273116.gene:9382335"/>
    <dbReference type="PaxDb" id="273116-14325759"/>
    <dbReference type="GeneID" id="1442209"/>
    <dbReference type="KEGG" id="tvo:TVG1573729"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="OrthoDB" id="56053at2157"/>
    <dbReference type="PhylomeDB" id="Q977Z7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, PADR-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="26"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="155" mass="17789" checksum="A0D2C6A1AAB008DA" modified="2001-10-01" version="1">MFGGMRGPCGHHVDRSRGFVGIRYWILRILADGEKTGADIMTTIENLTMGRWRPSPGAVYPILRALEEEGYIKGTERDNKKYYSLLEKGKEMIDMLGFGKFYGRDVETTNLYDAVERIGDYVNYLKDNSSKLKDDKDAIEKLRKIRDDIEGLIVQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q977Z9</accession>
    <name>RFCS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication factor C small subunit</fullName>
            <shortName evidence="1">RFC small subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Clamp loader small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rfcS</name>
        <name type="ordered locus">TV1518</name>
        <name type="ORF">TVG1570739</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60660" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60660.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917747.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q977Z9"/>
    <dbReference type="SMR" id="Q977Z9"/>
    <dbReference type="STRING" id="273116.gene:9382333"/>
    <dbReference type="PaxDb" id="273116-14325757"/>
    <dbReference type="GeneID" id="1442207"/>
    <dbReference type="KEGG" id="tvo:TVG1570739"/>
    <dbReference type="eggNOG" id="arCOG00469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_2_1_2"/>
    <dbReference type="OrthoDB" id="7928at2157"/>
    <dbReference type="PhylomeDB" id="Q977Z9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000952">
        <property type="entry name" value="Replication factor C subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01509">
        <property type="entry name" value="RfcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023748">
        <property type="entry name" value="Rep_factor-C_ssu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135987" description="Replication factor C small subunit">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="318" mass="35666" checksum="41BE7007C87E0884" modified="2005-08-30" version="2">MIEIWTEKYRPKSLSEIYGEDENIQKLKSFVEKKELPHLLFAGSVGTGKTSTAIALAIELFGESWKENFIEMNASNENGIDVIRNKIKDIARIRPSNPLGFKILFLDEADQLTAEAQAALRRTMEMYSETTRFVFACNYSSKIIPPIQSRTVVMRFRPVQDEFIKKKLNEIAKNEGFTIDDESMEAMVEVSGGDMRKAINVLQAVYTSGEISPKKIYEIIGYASPESVNKLISRAINGLFDEARQIVDKMMIEDGLSGIDIVKSVHSIVRASVVPPKQKIEIIKALADAEFRIVEGSNDRIQLDALIARIADIGSKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q978A1</accession>
    <name>Q978A1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG1567752 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1567752</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917745.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978A1"/>
    <dbReference type="STRING" id="273116.gene:9382330"/>
    <dbReference type="PaxDb" id="273116-14325755"/>
    <dbReference type="DNASU" id="1442205"/>
    <dbReference type="GeneID" id="1442205"/>
    <dbReference type="KEGG" id="tvo:TVG1567752"/>
    <dbReference type="eggNOG" id="arCOG01241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051789_0_0_2"/>
    <dbReference type="OrthoDB" id="359457at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="118"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="429" mass="49353" checksum="3B0B44AB863234C3" modified="2001-10-01" version="1">MTMQPKYRELLLDEDVRRWFENLKAKSVLTATVALRNLGHYCELTGTTPHGILNKARANEKDFRYEFTDFVRKMEKEGKAGSYIARFKKVILSWLKFNDIRLQLTVNIAGENETPTIVNERVPSKEELARILRKATSRGRVAIAIMAFSGLRPESLGDYEGTDGLRLGDLKELKLSDEIQFDKIPATVMVKSKLSKARHHYFSFIGEEGTTYIREYLDERRKQGEELTYESPLLQFDVRGVKKNAFLRTTLVTRDIREAIEQAGLKMRPYVLRAYFSTALDIAESKGLISHPWRQFIMGHKGDIEARYSTNKRLPPDIIDEMRESYNKCTKFLETRISDVSEENARLYLQQQLLSAVGYRQDEIDKMNLAETSTEDFQKLLRDKVAGAMASNGSKQRLVSMNEIEKLLGEGYEFQAVLPNGKAIMKMPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q978A9</accession>
    <name>Q978A9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1561939 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1561939</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917737.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978A9"/>
    <dbReference type="STRING" id="273116.gene:9382322"/>
    <dbReference type="PaxDb" id="273116-14325747"/>
    <dbReference type="GeneID" id="1442197"/>
    <dbReference type="KEGG" id="tvo:TVG1561939"/>
    <dbReference type="eggNOG" id="arCOG05334">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_465120_0_0_2"/>
    <dbReference type="OrthoDB" id="55786at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051162">
        <property type="entry name" value="T4SS_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121:SF6">
        <property type="entry name" value="SLR6007 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJGR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="593" mass="68457" checksum="51033FF123D55A21" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q978B1</accession>
    <name>Q978B1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1560588 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1560588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_021793813.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978B1"/>
    <dbReference type="STRING" id="273116.gene:9382320"/>
    <dbReference type="PaxDb" id="273116-14325745"/>
    <dbReference type="GeneID" id="24779987"/>
    <dbReference type="KEGG" id="tvo:TVG1560588"/>
    <dbReference type="HOGENOM" id="CLU_2406520_0_0_2"/>
    <dbReference type="OrthoDB" id="371748at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="92" mass="11095" checksum="C3D4927BD782F467" modified="2001-10-01" version="1">MSEKKRRGLDDLISQKPANVLERNAEQTEHRYTKTVGFKVTNRQYDTYRLYSKYYGPDELIEKWRSDLEKIAQEKGQELENVETEIRKRLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>Q978D3</accession>
    <name>Q978D3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1535972 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1535972</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978D3"/>
    <dbReference type="PaxDb" id="273116-14325723"/>
    <dbReference type="KEGG" id="tvo:TVG1535972"/>
    <dbReference type="HOGENOM" id="CLU_2949510_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="59" mass="6825" checksum="79B84E8F664274FC" modified="2001-10-01" version="1">MWILTDWITRKGSFQGVLEVTGCPGNIFSEGSMRNPYKYTFSGIYRSNLPTRKCEEPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q978E9</accession>
    <name>Q978E9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Transcription elongation factor Spt5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">spt5</name>
        <name evidence="9" type="ORF">TVG1517588</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal Spt5 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917699.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978E9"/>
    <dbReference type="STRING" id="273116.gene:9382280"/>
    <dbReference type="PaxDb" id="273116-14325707"/>
    <dbReference type="GeneID" id="1442159"/>
    <dbReference type="KEGG" id="tvo:TVG1517588"/>
    <dbReference type="eggNOG" id="arCOG01920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929443_0_0_2"/>
    <dbReference type="OrthoDB" id="371863at2157"/>
    <dbReference type="PhylomeDB" id="Q978E9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06091">
        <property type="entry name" value="KOW_NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NGN_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00950">
        <property type="entry name" value="Spt5_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011590">
        <property type="entry name" value="Spt5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00405">
        <property type="entry name" value="KOW_elon_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="7">
        <location>
            <begin position="133"/>
            <end position="228"/>
        </location>
    </feature>
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        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="288" mass="32215" checksum="F5C3F4F790A3F805" modified="2001-10-01" version="1">MESGKEYEWITFNADSLMERSSGEKIDFPLRIKNIQNVKRKFSFGIVYHIMEKDPTVDWKAVVTINKSEDISMMGDGEYSDDFDIEGNKEKVIGLTVYAPKGASANDTLDLNIEIESEDNVHHFTKDVKIVLKPVIVALKTTVGSEVQVATDLNNKAERDRDERSQGGEKQPSEVFAIMAPYEIKGYIFVETMHPDRISYLAKDIRGYKGMVSGKIDLEEIAHYLTPKPAVSGLELGSLVELVEGPFKGEKAKIISIDSVKEEVTVQLVESMVPIPVTVKAESIRLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q978G6</accession>
    <name>Q978G6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH marR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1497808</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978G6"/>
    <dbReference type="STRING" id="273116.gene:9382261"/>
    <dbReference type="PaxDb" id="273116-14325688"/>
    <dbReference type="DNASU" id="1442138"/>
    <dbReference type="GeneID" id="1442138"/>
    <dbReference type="KEGG" id="tvo:TVG1497808"/>
    <dbReference type="eggNOG" id="arCOG01692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078469_2_1_2"/>
    <dbReference type="OrthoDB" id="56972at2157"/>
    <dbReference type="PhylomeDB" id="Q978G6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="24044" checksum="57F67FA3770AD6FC" modified="2001-10-01" version="1">MSTENEIIILLKKDGPKRLDELAKELGISNMAVLRHMEKLEATGVVERRTFKGKQGRPYFKFSIKEIGASQITNSDGKILLDLISFLSENGHEKILDNFLEWRYSQQIIEYRKQMSGEDSDQKFLTLVKLRYDDCYFPEYSVRDGIYEITEYNCPIFRIAQKDGYACELERKLFENTTGFDVEYTHRQVDGAYSCKFILRKKPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q978H4</accession>
    <name>Q978H4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1490552 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1490552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054037.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978H4"/>
    <dbReference type="STRING" id="273116.gene:9382252"/>
    <dbReference type="REBASE" id="685106">
        <property type="entry name" value="TvoIVP"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325680"/>
    <dbReference type="GeneID" id="1442131"/>
    <dbReference type="KEGG" id="tvo:TVG1490552"/>
    <dbReference type="eggNOG" id="arCOG08993">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1141953_0_0_2"/>
    <dbReference type="OrthoDB" id="58109at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd22344">
        <property type="entry name" value="PDDEXK_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.210.20">
        <property type="entry name" value="MvaI/BcnI restriction endonuclease, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3570">
        <property type="entry name" value="MvaI/BcnI restriction endonuclease, recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029127">
        <property type="entry name" value="MvaI_BcnI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043004">
        <property type="entry name" value="MvaI_BcnI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043005">
        <property type="entry name" value="MvaI_BcnI_rec"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15515">
        <property type="entry name" value="MvaI_BcnI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MvaI/BcnI restriction endonuclease" evidence="1">
        <location>
            <begin position="7"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="227" mass="26046" checksum="036E6902B5C8D5B4" modified="2001-10-01" version="1">MDINQFIEKFKQIETKGFVKSMRRGPTGIGMTLESLLGIKENNISLPDLDGIELKAHRDNSMNLITLFTFNRGAWIMNPLEAVRKYGTLDKNGRLGLYFTMNFTPNSAGLYLFADNEAVSVRHINGDVLVKWNFNDLEDQFKRKVPALIIAYARTEYRGNDEYFQFYKAVLLKGTTKDIIRESIKNNIILIDLRLHDKGTMARNHGTGFRIYEKNLPLVFSNTEVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q978H9</accession>
    <name>Q978H9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transposase InsH N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1483393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917668.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978H9"/>
    <dbReference type="PaxDb" id="273116-14325675"/>
    <dbReference type="GeneID" id="1442126"/>
    <dbReference type="KEGG" id="tvo:TVG1483393"/>
    <dbReference type="eggNOG" id="arCOG06508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1280857_0_0_2"/>
    <dbReference type="OrthoDB" id="148050at2157"/>
    <dbReference type="PhylomeDB" id="Q978H9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35604">
        <property type="entry name" value="TRANSPOSASE INSH FOR INSERTION SEQUENCE ELEMENT IS5A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35604:SF2">
        <property type="entry name" value="TRANSPOSASE INSH FOR INSERTION SEQUENCE ELEMENT IS5A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase InsH N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="215" mass="25012" checksum="532FCAF9100D282F" modified="2001-10-01" version="1">MVKVLFLQSVYNLVDEAMEREMHYRVSFMNFLNYPDSVPDSRAIWLFRERLSSTGKDKMIWEPFESEGITVKKGVVQDASFIESDPGKHGRKKPPVSPNMPEIVQDEKKDKTMTKDGKRQAKREAKIRVAEKKRIRRDERRNAKTRRFKDGTWAKKGKRSHFGNKLHTVQGTDIPLIMEFVLTTAFSHDSKVDLGIPGIPTYRDKRIFRITNQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q978I5</accession>
    <name>Q978I5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1476274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917662.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978I5"/>
    <dbReference type="STRING" id="273116.gene:9382240"/>
    <dbReference type="PaxDb" id="273116-14325669"/>
    <dbReference type="GeneID" id="1442120"/>
    <dbReference type="KEGG" id="tvo:TVG1476274"/>
    <dbReference type="eggNOG" id="arCOG01793">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387532_0_0_2"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="PhylomeDB" id="Q978I5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 12" evidence="1">
        <location>
            <begin position="47"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="197" mass="22702" checksum="B39318977F252004" modified="2001-10-01" version="1">MDLYREGEEKFGPITSHFYEIISNHFLRNLYSMITEDIDKFKPETILDVGCGTGTADFFIANSFSNVIIDCIDPSPYMLSIASKKLSRFKGRVNFALGSSRSIPFEKKYDFVFSSISFHHWKCRENGLKTMGLHVKDNGVLAIYEYYRPNLRSFHKAAGKHALSENEIEGLNVDGFEKIYEINGEMVRILFLKNDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q978I6</accession>
    <name>Q978I6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD/PDEase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1474209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760282.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978I6"/>
    <dbReference type="STRING" id="273116.gene:9382238"/>
    <dbReference type="PaxDb" id="273116-14325668"/>
    <dbReference type="GeneID" id="1442119"/>
    <dbReference type="KEGG" id="tvo:TVG1474209"/>
    <dbReference type="eggNOG" id="arCOG04430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026821_3_1_2"/>
    <dbReference type="PhylomeDB" id="Q978I6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="1">
        <location>
            <begin position="51"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="370" mass="42067" checksum="7F00E175B130001A" modified="2001-10-01" version="1">MTIIMVKIIQDPVFGPIKADGVILDLIDSPEFQRLRRIKNLGLCSLVFPGANHTRFEHSIGTYYLASLYNEHLRTMSDELKIAALLHDIGHFPYSHTIEEFYMETEKIDHLQAGINLIEGKAESEIPAILEKHGIDPHWVSSILKGISNVLSEIISGPLDADELDYLRRDSFYCGVSIGYVNPLRIIDVSSVYEGQIVSEEKGLSDIESLLISRFLMYQAVYFHKTCRIANKMLGIAARLAEAIGTSRMVDEELMALLLSKKRSERIARDIINRRLMKVIFKEKYGEELYRDITDRIGEDAIVDVIPPLSFSGRERLKTDVSVKIQGRIVAGEEISPLVNSLNQAIERRYVYVYGYKEDEEKIKSDLRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q978L9</accession>
    <name>Q978L9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1441685 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1441685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054032.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978L9"/>
    <dbReference type="STRING" id="273116.gene:9382205"/>
    <dbReference type="PaxDb" id="273116-14325635"/>
    <dbReference type="GeneID" id="24779975"/>
    <dbReference type="KEGG" id="tvo:TVG1441685"/>
    <dbReference type="HOGENOM" id="CLU_2930411_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="60" mass="7041" checksum="EF0A65C311714D1D" modified="2001-10-01" version="1">MPNIHIEDEVYRHLIERSDSPEDLANQILRSFLMAPKVYSVEKGEYQKYLEGYVMHGNAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q978R7</accession>
    <name>Q978R7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-terminal acetyltransferase complex subunit [ARD1]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1394372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917582.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978R7"/>
    <dbReference type="STRING" id="273116.gene:9382156"/>
    <dbReference type="PaxDb" id="273116-14325587"/>
    <dbReference type="GeneID" id="1441464"/>
    <dbReference type="KEGG" id="tvo:TVG1394372"/>
    <dbReference type="eggNOG" id="arCOG00826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_19_1_2"/>
    <dbReference type="OrthoDB" id="55684at2157"/>
    <dbReference type="PhylomeDB" id="Q978R7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43415:SF3">
        <property type="entry name" value="GNAT-FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43415">
        <property type="entry name" value="SPERMIDINE N(1)-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="177" mass="20436" checksum="E22EA1621005DB2E" modified="2001-10-01" version="1">MLWKKAQEETHDYIIRKADPSDAAGIISCMQSVMDERIYLVGEYYLLSERAEQERIKSLDDLTLVCVIDRKVVGVLTVQRGIYRKNRHTGMLGIAIMAGHRHKGLGTRMIKEAILWCEDQGIKKLNLEVFSTNVNAIKTYEKVGFEVEGKRRKQFLIDGQYVDDVLMTYYVDKSLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q978S2</accession>
    <name>Q978S2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="2 9">6.1.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG1388781</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2 9"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917578.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978S2"/>
    <dbReference type="STRING" id="273116.gene:9382151"/>
    <dbReference type="PaxDb" id="273116-14325582"/>
    <dbReference type="DNASU" id="1441460"/>
    <dbReference type="GeneID" id="1441460"/>
    <dbReference type="KEGG" id="tvo:TVG1388781"/>
    <dbReference type="eggNOG" id="arCOG00487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_6_1_2"/>
    <dbReference type="OrthoDB" id="372102at2157"/>
    <dbReference type="PhylomeDB" id="Q978S2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF5">
        <property type="entry name" value="ARGININE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR anticodon binding" evidence="11">
        <location>
            <begin position="433"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB60485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="545" mass="63398" checksum="149A0E3E9A7B9573" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q978S8</accession>
    <name>Q978S8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1381158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917572.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978S8"/>
    <dbReference type="STRING" id="273116.gene:9382145"/>
    <dbReference type="PaxDb" id="273116-14325576"/>
    <dbReference type="GeneID" id="1441454"/>
    <dbReference type="KEGG" id="tvo:TVG1381158"/>
    <dbReference type="eggNOG" id="arCOG01617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_2"/>
    <dbReference type="OrthoDB" id="7236at2157"/>
    <dbReference type="PhylomeDB" id="Q978S8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF4">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="334" mass="37960" checksum="A3645CD0F20C7FE3" modified="2001-10-01" version="1">MKYVRLGSSGTLVSQLALGTWHLPGSNRYSSDGVEKVDESEFQRIFKKAYDAGINFFDTANIYHGRVEKNEDHIDHIGNSERILGKAIKGYERESLVIATKVRGPVSKFINGEGLSRKHIMWQIKESLQRLGLEYVDLYQIHWSDSLTPHEETARVLSHIVDLDMARYIGESNHSAEDIVDFMNISEKLNLHHFVTMQEPYNILQRGIEKDKIPVAKKYGMGILAYVPLAQGVLTGKYVSGPEKGSRSSYYPEIIDFHQKNQKKIDALINFAKEKDITGGQLAIAWLIKKSQIEGIPIIPLLGITKEKYLEENLKALEVNLTDSDMREIDRMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q978T1</accession>
    <name>Q978T1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1377296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978T1"/>
    <dbReference type="STRING" id="273116.gene:9382142"/>
    <dbReference type="PaxDb" id="273116-14325573"/>
    <dbReference type="KEGG" id="tvo:TVG1377296"/>
    <dbReference type="eggNOG" id="arCOG01843">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2115609_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978T1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF25">
        <property type="entry name" value="SCP2 STEROL-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="25"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="114" mass="12362" checksum="841ECB3B6DCBB1CE" modified="2001-10-01" version="1">MILYFTMGSKELINEMVSKVNEKDLSGIANAEKIVSIALSDAKPFTIIIKNGKIQLTDETPEKADATVKCSDQTLSDIITGKLNAFSAFMSGKVTVSGDLFFVQKIISVIGKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q978T9</accession>
    <name>Q978T9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1368057</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054028.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978T9"/>
    <dbReference type="STRING" id="273116.gene:9382134"/>
    <dbReference type="PaxDb" id="273116-14325565"/>
    <dbReference type="GeneID" id="1441443"/>
    <dbReference type="KEGG" id="tvo:TVG1368057"/>
    <dbReference type="eggNOG" id="arCOG00558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_28_3_2"/>
    <dbReference type="OrthoDB" id="4631at2157"/>
    <dbReference type="PhylomeDB" id="Q978T9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF13">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="33"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="226"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="373" mass="41731" checksum="06E300D5882ECE20" modified="2001-10-01" version="1">MKGFEEFNLRNELIESIRGTGYSEPTEVQSMAIPIALAGSDLVVRSKTGSGKTAAYLIPIINNTAKEKGIRALILLPTRELAVQVAKVSEALGKRSGIRTVVVYGGVSINKQIELILRGANIIVGTPGRTLDLIDRGILNFDKVSYFVLDEADEMLDMGFIEDIKKIINVLPVERQSFLFSATIPSEIIELAKGFMHNEEILFLSKDEVTVNGIDHNYAVSRRERKLRTLFSYIDKYKPEKSIIFSRTKAGANMIYEALINHGQDAVIMHGDLTQAQREKALYRFKNFGRFLVATNVAARGLDIGGISDIINYDVPDDPRVYVHRVGRTARMGAAGRAFTIVEDREIGSIDMIRHEARVKMKEIHLETTRKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q978U2</accession>
    <name>Q978U2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Proline permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1363800</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917558.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978U2"/>
    <dbReference type="STRING" id="273116.gene:9382131"/>
    <dbReference type="PaxDb" id="273116-14325562"/>
    <dbReference type="GeneID" id="1441440"/>
    <dbReference type="KEGG" id="tvo:TVG1363800"/>
    <dbReference type="eggNOG" id="arCOG01316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_15_0_2"/>
    <dbReference type="PhylomeDB" id="Q978U2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050277">
        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF8">
        <property type="entry name" value="MONOCARBOXYLIC ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086">
        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="468"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="507" mass="55099" checksum="8C9A1348A9AF1387" modified="2001-10-01" version="1">MIDTVEISVFLVFFIVFIAIGFLAKNFRKGNLNQLPEWALGGRRLGTFLVWFLVGADLYTAYTFIAVPSSMYSVGSVYFFAVPYVSLAFAIAMLTMPKLWAIAKEKGYVTAGDFVKDRFNSESLAIVIALTGIVAELPYIALQIVGMRAILTALLHPVGGNVTLISNIALVLAFLALIAFTYTSGLRGASLTAVMKDIIIFTTVIVVIVVALVAYHSAFTTAFTVKPVYADLPPKLYNAYWSLFLMSAAALYLYPHAINGSLSSMSAKKLRLSTALLPIYGIGLAFLALFGVIVYGIPAAESALKIYPSSIQGALVVPTIIASVTPQWFTGFAFLGIFIGGLVPAAIMSMSQATLLTRNVIRYVNRNLEEKRETFIAKLSMVFFQLLALGFVFIVPETYAISLQLIGGIIILQTLPAVFLGMYVKHLEKYAIAAGWLAGIASGVYFEIYSNFVYLHYSIIKTTLMPSAFGLIFVGLTALVINLVVVVIGTAIVYAMHKQPNISKEKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="150" xmlns="https://uniprot.org/uniprot">
    <accession>Q978W6</accession>
    <name>KAE1B_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.234</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>N6-L-threonylcarbamoyladenine synthase</fullName>
                <shortName>t(6)A synthase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">tRNA threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Serine/threonine-protein kinase Bud32</fullName>
                <ecNumber evidence="1">2.7.11.1</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name type="ordered locus">TV1299</name>
        <name type="ORF">TVG1340828</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917534.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978W6"/>
    <dbReference type="SMR" id="Q978W6"/>
    <dbReference type="STRING" id="273116.gene:9382106"/>
    <dbReference type="PaxDb" id="273116-14325538"/>
    <dbReference type="DNASU" id="1441416"/>
    <dbReference type="GeneID" id="1441416"/>
    <dbReference type="KEGG" id="tvo:TVG1340828"/>
    <dbReference type="eggNOG" id="arCOG01183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_2_2_2"/>
    <dbReference type="OrthoDB" id="6818at2157"/>
    <dbReference type="PhylomeDB" id="Q978W6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24131">
        <property type="entry name" value="ASKHA_NBD_Kae1_arch_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000038">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01447">
        <property type="entry name" value="Kae1_Bud32_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009220">
        <property type="entry name" value="tRNA_threonyl_synthase/kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007174">
        <property type="entry name" value="PRK09605.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036401">
        <property type="entry name" value="Gcp_STYKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000303662" description="Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein">
        <location>
            <begin position="1"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="330"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Kae1">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for kinase activity" evidence="1">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="333"/>
            <end position="341"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01447"/>
        </source>
    </evidence>
    <sequence length="527" mass="58291" checksum="E66A4EF292645941" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Y9</accession>
    <name>Q978Y9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="2">M1Pi</shortName>
            <shortName evidence="2">MTR-1-P isomerase</shortName>
            <ecNumber evidence="2">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">MTNA-like protein</fullName>
            <shortName evidence="2">aMTNA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1316233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917510.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Y9"/>
    <dbReference type="STRING" id="273116.gene:9382081"/>
    <dbReference type="PaxDb" id="273116-14325514"/>
    <dbReference type="GeneID" id="1441392"/>
    <dbReference type="KEGG" id="tvo:TVG1316233"/>
    <dbReference type="eggNOG" id="arCOG01123">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_1_2_2"/>
    <dbReference type="OrthoDB" id="45195at2157"/>
    <dbReference type="PhylomeDB" id="Q978Y9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000006">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004326">
        <property type="entry name" value="PRK05720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1 2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="55"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="234"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="340" mass="37758" checksum="2C3ED35319FDDBA6" modified="2001-10-01" version="1">MKVIIDGEVRTLKAAWYEDGEVKLIDQRELPDKIKIFSAKNSDDIAYAIKNMVVRGAPAIGVTAAYGLAMASKNGEDMNKAKEKIRATRPTAYDLFKAIRYMEVNGYDMNAARRYAMEIEGRSKKIGELGNELIRNGAKILTHCNAGALAVLDWGTALSPMRIAHNEGKNIFVFVDETRPRLQGAKLTAWELAQEGIDHAIIADNAAGFYMRRKEIDLVIVGADRIASNGDFANKIGTYEKAVLAKVNGIPFYVAAPGSTFDFSIRSGDEIPIEERDENEVLEINGIRIGPNESHSKNPAFDVTPNEYVTGFITEYGIFKPNELNKLKDLMDKDLFMNIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q979A3</accession>
    <name>Y1259_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0292 protein TV1259</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV1259</name>
        <name type="ORF">TVG1298026</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds two Mg(2+) per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0292 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979A3"/>
    <dbReference type="SMR" id="Q979A3"/>
    <dbReference type="STRING" id="273116.gene:9382064"/>
    <dbReference type="PaxDb" id="273116-14325497"/>
    <dbReference type="KEGG" id="tvo:TVG1298026"/>
    <dbReference type="eggNOG" id="arCOG01486">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140789_1_0_2"/>
    <dbReference type="OrthoDB" id="56459at2157"/>
    <dbReference type="PhylomeDB" id="Q979A3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01027">
        <property type="entry name" value="TOPRIM_RNase_M5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01095">
        <property type="entry name" value="UPF0292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034141">
        <property type="entry name" value="TOPRIM_RNase_M5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022972">
        <property type="entry name" value="UPF0292"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39964:SF2">
        <property type="entry name" value="UPF0292 PROTEIN MJ1624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39964">
        <property type="entry name" value="UPF0292 PROTEIN TK1411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110455">
        <property type="entry name" value="Toprim domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000143958" description="UPF0292 protein TV1259">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="11"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01095"/>
        </source>
    </evidence>
    <sequence length="119" mass="14184" checksum="29F1F7BEEB874E54" modified="2001-10-01" version="1">MMRRYRRKNSSIPIIVEGRNDLASLRKLSFYGEIIVFNRGISITSFSDEISNKYREVIILTDFDNKGSFLKDRFYGYLTGMGVYVDLYLWNFIRKHVPIRTVEELPAEFEREVEKEIQM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q979A8</accession>
    <name>RNZ_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease Z</fullName>
            <shortName evidence="1">RNase Z</shortName>
            <ecNumber evidence="1">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnz</name>
        <name type="ordered locus">TV1254</name>
        <name type="ORF">TVG1292892</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase Z family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60396" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60396.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917488.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979A8"/>
    <dbReference type="SMR" id="Q979A8"/>
    <dbReference type="STRING" id="273116.gene:9382059"/>
    <dbReference type="PaxDb" id="273116-14325492"/>
    <dbReference type="GeneID" id="1441370"/>
    <dbReference type="KEGG" id="tvo:TVG1292892"/>
    <dbReference type="eggNOG" id="arCOG00501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031317_2_1_2"/>
    <dbReference type="OrthoDB" id="85118at2157"/>
    <dbReference type="PhylomeDB" id="Q979A8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000801">
        <property type="entry name" value="PRK00055.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02651">
        <property type="entry name" value="RNase_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018:SF2">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000155940" description="Ribonuclease Z">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="304" mass="34316" checksum="A331167B7F2C44E5" modified="2004-01-16" version="2">MASNIKIVFFGTGGSWPTPIRAMPGVGIQIDDVFNLFDCGEGTQKQIMKSKWSFMSIDNIFITHFHGDHFLGLIGLVQSMSFNNRTKDLNIFGPRGAIGIISNAINIGYYTLRFRINVYELEPDKTYDLGKFLLKTTLNDHPVPALSYTIEEKDIIRVDPQKAKELGIPSKIIEKIRDNGTYEYRGRKYSIDEISGGIRKGRKIVYTGDTKPMQKMADFAKHADVLIHDTTTDSSFEPAVNQFGHSSAKQAARIARDAGVSRLFLYHYSPRITDVSPLVDDARAEFQESYASEDLMEYEVKVKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q979B6</accession>
    <name>Q979B6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TVG1285073 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1285073</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917479.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979B6"/>
    <dbReference type="STRING" id="273116.gene:9382050"/>
    <dbReference type="PaxDb" id="273116-14325483"/>
    <dbReference type="GeneID" id="1441361"/>
    <dbReference type="KEGG" id="tvo:TVG1285073"/>
    <dbReference type="eggNOG" id="arCOG03651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2285195_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="19"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="101" mass="10950" checksum="3537EF92ADB469B1" modified="2001-10-01" version="1">MVRKRTGLKKNAIGLMQGVFQSMGKVAPAADIAILLVATFSIADSKTILSVIFGWLIYALFMVTPYQFSKYKSNAGSYYAYASASTESGKLGPITALSFMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q979H4</accession>
    <name>Q979H4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH marR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1216673</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917420.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979H4"/>
    <dbReference type="STRING" id="273116.gene:9381988"/>
    <dbReference type="PaxDb" id="273116-14325425"/>
    <dbReference type="GeneID" id="1441302"/>
    <dbReference type="KEGG" id="tvo:TVG1216673"/>
    <dbReference type="eggNOG" id="arCOG03182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_4_2"/>
    <dbReference type="OrthoDB" id="10712at2157"/>
    <dbReference type="PhylomeDB" id="Q979H4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42756">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42756:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OF EMRAB OPERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH marR-type" evidence="4">
        <location>
            <begin position="9"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="143" mass="16570" checksum="D22B1E114D145F59" modified="2001-10-01" version="1">MIEEKCTNLMDIWHMFIRVFNLSKKKMGESLSHISAKPIEVRILYLLSEDESTVNKLAELTDVTPAWITGTLDEMESQGLIVRSRSGEDRRVVNVHITEKGIEVLNESQKVYLDFLKRSLSSLTDSELDEFRRILKKIEETLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q979I2</accession>
    <name>Q979I2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1208678 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1208678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917412.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979I2"/>
    <dbReference type="STRING" id="273116.gene:9381980"/>
    <dbReference type="PaxDb" id="273116-14325417"/>
    <dbReference type="GeneID" id="1441294"/>
    <dbReference type="KEGG" id="tvo:TVG1208678"/>
    <dbReference type="eggNOG" id="arCOG07373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2766327_0_0_2"/>
    <dbReference type="OrthoDB" id="55983at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="69" mass="8487" checksum="76F88F981029A1B7" modified="2001-10-01" version="1">METDQAVDKIKRDLEERYGKIDDIRPERLKFDETLKEYSMIVRFKLENEERVVVYYFSKDGNILRHFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="141" xmlns="https://uniprot.org/uniprot">
    <accession>Q979J0</accession>
    <name>Q979J0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transport system ATP-binding protein SP1P2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1199143</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917404.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979J0"/>
    <dbReference type="STRING" id="273116.gene:9381972"/>
    <dbReference type="PaxDb" id="273116-14325409"/>
    <dbReference type="GeneID" id="1441286"/>
    <dbReference type="KEGG" id="tvo:TVG1199143"/>
    <dbReference type="eggNOG" id="arCOG00175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="OrthoDB" id="18368at2157"/>
    <dbReference type="PhylomeDB" id="Q979J0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000042">
        <property type="entry name" value="Maltose/maltodextrin ABC transporter, ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047641">
        <property type="entry name" value="ABC_transpr_MalK/UgpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875">
        <property type="entry name" value="MALTODEXTRIN IMPORT ATP-BINDING PROTEIN MSMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875:SF15">
        <property type="entry name" value="TREHALOSE IMPORT ATP-BINDING PROTEIN SUGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="371" mass="41575" checksum="12D437021CDFDC0D" modified="2001-10-01" version="1">MGVRIENLTVKYGDKTVIERMNLDVKDGEFFVILGSSGSGKTTLLRSIAGLTEISGGHIYIDDIDVTDFYPSDRNIAMVFQNFALYPHMTVFDNIALNLKIKHLPRDQIKKRVEEVASLLHIEKQLKKYPKQLSGGEQQRVGIARALVRDPSVFLMDEPLSNLDAKLRREMLGELRSFHERVRKTTIYVCHDQDEAMALADRIMVLNSGEPAQIGTPDDLYIHPTNLFVASFIGNPPMNLIECDIIKDESSSEIVLNGVPITTVDHVPGDHAILGIRPEKVNISLKNGVPASFDYFINSGVNVEVHLDMNNILVRALIPREELKLNLRDLSHGDAVFIEIKPGPIYLFSKDSGNLITEVTYDSARNVEKKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-23" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q979K1</accession>
    <name>RS9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>30S ribosomal protein S9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rps9</name>
        <name type="ordered locus">TV1160</name>
        <name type="ORF">TVG1187879</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917394.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979K1"/>
    <dbReference type="SMR" id="Q979K1"/>
    <dbReference type="STRING" id="273116.gene:9381960"/>
    <dbReference type="PaxDb" id="273116-14325398"/>
    <dbReference type="GeneID" id="1441276"/>
    <dbReference type="KEGG" id="tvo:TVG1187879"/>
    <dbReference type="eggNOG" id="arCOG04243">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_2"/>
    <dbReference type="OrthoDB" id="52677at2157"/>
    <dbReference type="PhylomeDB" id="Q979K1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_A">
        <property type="entry name" value="Ribosomal_uS9_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019958">
        <property type="entry name" value="Ribosomal_uS9_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03627">
        <property type="entry name" value="uS9_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000111478" description="Small ribosomal subunit protein uS9">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="132" mass="14487" checksum="0559924C3E6CB0C2" modified="2001-10-01" version="1">MDYVITTGKRKTAVARAVVKKGKGIITINGTPAELYPVEVLRNKILEPVKLAEDKAKGIDVTVKVKGGGVTGQADASRTAIARGIVKFLQDNELENLFRQYDRTLIVNDVRIKLPKKPGGRGARAKKQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q979L2</accession>
    <name>Q979L2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1178481</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979L2"/>
    <dbReference type="STRING" id="273116.gene:9381948"/>
    <dbReference type="PaxDb" id="273116-14325387"/>
    <dbReference type="KEGG" id="tvo:TVG1178481"/>
    <dbReference type="eggNOG" id="arCOG00475">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057297_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q979L2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="295" mass="32995" checksum="8B60672BC627D95A" modified="2001-10-01" version="1">MQSVCAFIFILITQGNKIIRKVYCFALIHSPKMYSSSLKELRFVRSVEIVPSRNQGLEELAHSLDSLEGKANALTSPENPRGSPGIDPIITLFFLSKDRNIIPIPHITPRDKNKVHIISQINTANKFGINNFFVIGGDPINPKYESHEVREIDPIDLIKLIKANSGSTVGAALNPYRNVEEEIAWAKKNAGADFFITQAIYSSDALSMDWLKKRNFKLIAGFLPLIRKNQVEFSEKLGIFLPKVVKEKLLNSEDIRSTSVNIILNVFDEVKDFVDGIHIMPLGHNDIAADILGSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q979L5</accession>
    <name>PRIS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="1">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">priS</name>
        <name type="synonym">priA</name>
        <name type="ordered locus">TV1145</name>
        <name type="ORF">TVG1172230</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979L5"/>
    <dbReference type="SMR" id="Q979L5"/>
    <dbReference type="STRING" id="273116.gene:9381944"/>
    <dbReference type="PaxDb" id="273116-14325383"/>
    <dbReference type="KEGG" id="tvo:TVG1172230"/>
    <dbReference type="eggNOG" id="arCOG04110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056123_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q979L5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023639">
        <property type="entry name" value="DNA_primase_ssu_PriS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001641">
        <property type="entry name" value="PRK00419.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000046757" description="DNA primase small subunit PriS">
        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <sequence length="333" mass="38387" checksum="941770B4CA689AB5" modified="2001-10-01" version="1">MMRDFVSEEKLKLLFEKYYRSTDIDPPALITKREVGFMTFEGEIIRHMHLSNKFELNNMLRTTVPRHVYSSAAYYKKPDEKKMPEKIWEGADLIFDLDSDHLPGAEKMTYEEMLNSIKEQTKRLVNKFLVSDLGISEKDIRIYFSGSRGYHVHVSSEDVYPLGSDARREITDYVSGNSLSISVIDKALKTGEKRPGGWIKDVISKLYELGINAEKISQKQIERAIDQVKSHNATMVDAPVTYDIHRLIRMPQSLHGKSGMMVKEVDLDKFDDFDPLSDAIPKIFLEGDYDINVHEKTRKLRLLDYKIDLPPGRNRVPQYVAIFLVSSGRADFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q979L7</accession>
    <name>Q979L7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF72 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1170820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917377.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979L7"/>
    <dbReference type="STRING" id="273116.gene:9381942"/>
    <dbReference type="PaxDb" id="273116-14325381"/>
    <dbReference type="DNASU" id="1441259"/>
    <dbReference type="GeneID" id="1441259"/>
    <dbReference type="KEGG" id="tvo:TVG1170820"/>
    <dbReference type="eggNOG" id="arCOG04291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046519_0_1_2"/>
    <dbReference type="OrthoDB" id="35747at2157"/>
    <dbReference type="PhylomeDB" id="Q979L7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="entry name" value="Protein of unknown function UPF0759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348:SF4">
        <property type="entry name" value="DUF72 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YECE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="TM1631-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="29934" checksum="5753E258674F9503" modified="2001-10-01" version="1">MIFVGCSGWSYEEWNGVFYPKSKINKLKFYSTMFNTVEVNSTFYREINNDIINRWIETVRGKNFIFSIKVPQNITHKAILEKDSNSKSLYETFIDKTVRPIRNARMLGAVLVQLPPSFNSNLQTELLDTICCENEYRTAVEVRNKDLYSNEELRKELEKRGIAIVDVDSTEHPLSNLNSGLKWSYVRLHGRAQLGWKSQNPYDYRYSEQEISDLSSKIMAKNYDEIFVYFNNHPRGSAPINALKMASCLGISNNWFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q979L9</accession>
    <name>GATE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit E</fullName>
            <shortName evidence="1">Glu-ADT subunit E</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatE</name>
        <name type="ordered locus">TV1141</name>
        <name type="ORF">TVG1166951</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatE subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917375.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979L9"/>
    <dbReference type="SMR" id="Q979L9"/>
    <dbReference type="STRING" id="273116.gene:9381940"/>
    <dbReference type="PaxDb" id="273116-14325379"/>
    <dbReference type="GeneID" id="1441257"/>
    <dbReference type="KEGG" id="tvo:TVG1166951"/>
    <dbReference type="eggNOG" id="arCOG01719">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030702_0_0_2"/>
    <dbReference type="OrthoDB" id="7316at2157"/>
    <dbReference type="PhylomeDB" id="Q979L9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00588">
        <property type="entry name" value="GatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004414">
        <property type="entry name" value="GatE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00134">
        <property type="entry name" value="gatE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003107">
        <property type="entry name" value="PRK04028.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF2">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140085" description="Glutamyl-tRNA(Gln) amidotransferase subunit E">
        <location>
            <begin position="1"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00588"/>
        </source>
    </evidence>
    <sequence length="598" mass="67355" checksum="6777EB8F039F8CCF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q979M2</accession>
    <name>Q979M2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH dehydrogenase I chain C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1165354</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917372.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979M2"/>
    <dbReference type="STRING" id="273116.gene:9381937"/>
    <dbReference type="PaxDb" id="273116-14325376"/>
    <dbReference type="GeneID" id="1441254"/>
    <dbReference type="KEGG" id="tvo:TVG1165354"/>
    <dbReference type="eggNOG" id="arCOG01551">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042628_6_3_2"/>
    <dbReference type="OrthoDB" id="43567at2157"/>
    <dbReference type="PhylomeDB" id="Q979M2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase, 30kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010218">
        <property type="entry name" value="NADH_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01961">
        <property type="entry name" value="NuoC_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004737">
        <property type="entry name" value="PRK06074.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="Nqo5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase 30kDa subunit" evidence="3">
        <location>
            <begin position="17"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="151" mass="17503" checksum="68C26F3298D702FB" modified="2001-10-01" version="1">MVDILGEEKGKDGRRIIKVAKENLLALMRDLKAQGYNLSLTTGVDYVDHIEVVYHLYNLEKNEYIIVKTETTDNHVPSLTPLWNAANWDEREEYDLVGIVFDGHPYLKRLFLPEGWVGHPLRKNYDLSKAQYVNMDEEGNDYVTFDPEGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q979P1</accession>
    <name>Q979P1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1150731</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917353.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979P1"/>
    <dbReference type="STRING" id="273116.gene:9381918"/>
    <dbReference type="PaxDb" id="273116-14325357"/>
    <dbReference type="GeneID" id="1441235"/>
    <dbReference type="KEGG" id="tvo:TVG1150731"/>
    <dbReference type="eggNOG" id="arCOG02172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111016_3_0_2"/>
    <dbReference type="OrthoDB" id="6529at2157"/>
    <dbReference type="PhylomeDB" id="Q979P1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006113">
        <property type="entry name" value="PTP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR006113-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="159" mass="18264" checksum="5DEE81316E56A7F2" modified="2001-10-01" version="1">MKLTVNGWYTNMRFSAAHFIPSHYKCSRLHGHDYGIIINVDGEMQDGMLIDFIELKNAIRSVINEMDHKLLVPGKANIAKYNESTNEYEISYNDKRMIIPAEYVYICDVYNTTSEELSRFVAEKVGAKIGKRKNLRSIEVSVEEGPGQGVFYSVDMRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Q6</accession>
    <name>Q979Q6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Succinyl-diaminopimelate desuccinylase / N-acetylornithine deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1135544</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054019.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Q6"/>
    <dbReference type="STRING" id="273116.gene:9381902"/>
    <dbReference type="PaxDb" id="273116-14325342"/>
    <dbReference type="GeneID" id="1441219"/>
    <dbReference type="KEGG" id="tvo:TVG1135544"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_2_2_2"/>
    <dbReference type="PhylomeDB" id="Q979Q6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010589">
        <property type="entry name" value="PRK13983.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF32">
        <property type="entry name" value="ARGE_DAPE-RELATED DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0170">Cobalt</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="8">
        <location>
            <begin position="188"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="397" mass="44844" checksum="68A4978F2C70D3B4" modified="2001-10-01" version="1">MDLYQMEDRDFIIEVARRIIRIPSISPASGGEGESKRADEIGRILSELGYTDFKRYDTKDDTGTVRSNIVLKIGNNSKTLWLIAHIDTVPVGDPSLWTKPPFELTVEGDKMYGRGTEDDGQAVFTALLILRDLKKIRMNYNFGIAFVADEEVGSKYGIQYLLTKDVFGKDDLIIVPDAGSEDGLNIEISEKSILWLKFRVIGKQYHASMPPNAINSFRESAKFILKLDKTLHAKYSAIDKLYNVPYSTFEPTKHEKNVDNINTIPGTDVFYYDCRVLPQYSLDDVISTVEAEISDFQKESNAKIEYSVMQKEQSPPKTPEDSEVVKKLVSSIRKLRKKEPNVLGIGGGTCAAFFRQLGLPAVVWYTTIEENAHKPDEFCLISHILMDRDVIEDMLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q979R6</accession>
    <name>Q979R6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1123753 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1123753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979R6"/>
    <dbReference type="STRING" id="273116.gene:9381891"/>
    <dbReference type="PaxDb" id="273116-14325332"/>
    <dbReference type="KEGG" id="tvo:TVG1123753"/>
    <dbReference type="eggNOG" id="arCOG07398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_318491_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF57">
        <property type="entry name" value="CARBOXYPEPTIDASE D, B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49478">
        <property type="entry name" value="Cna protein B-type domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="913" mass="101406" checksum="59E551C4E7A00C30" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q979S3</accession>
    <name>Q979S3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1116776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917318.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979S3"/>
    <dbReference type="PaxDb" id="273116-14325325"/>
    <dbReference type="GeneID" id="1441200"/>
    <dbReference type="KEGG" id="tvo:TVG1116776"/>
    <dbReference type="eggNOG" id="arCOG06047">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_728857_0_0_2"/>
    <dbReference type="OrthoDB" id="57234at2157"/>
    <dbReference type="PhylomeDB" id="Q979S3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="121"/>
        </location>
    </feature>
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        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="10"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="391" mass="41524" checksum="4653B71CBA9EF3FE" modified="2001-10-01" version="1">MASSTKAYAPLVSYWIMLFAIGMGWFILSPLVPSIVQSLNVQTSLVIFIITLYGYAMVIFGLVAGYISAVRNVYNSLLASAILTFVGLTVRAFVNRFDLFFSFSVIAAVGYPLAMAPVGSVADSISPKSAHTVIGISVGLLFFGMAVGSFTAPGLEEHFGLKFTLIVPAILSAISLVLIASMHSSFPRYYRARSLKGSFSVGMVKNWYVGLSIASMSVMFSSIAASVLIMHNINHLQALNYAGIFGGLAFVGSAFGAVLLPLIFGKYGGLKIGLIVSGLLSFFSISILAILISLTRLLSAIAVSYFIFGFFGNAYWSMAMTSVTYYASSPDKAGLSTAMFSVASNAGVAVIPVFLGTAFLRQSTLILGVSIVLAMEFVSAVLAFTLKYKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q979U0</accession>
    <name>Q979U0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ammonium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1095610</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917300.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979U0"/>
    <dbReference type="STRING" id="273116.gene:9381865"/>
    <dbReference type="PaxDb" id="273116-14325308"/>
    <dbReference type="GeneID" id="1441182"/>
    <dbReference type="KEGG" id="tvo:TVG1095610"/>
    <dbReference type="eggNOG" id="arCOG04397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_1_2"/>
    <dbReference type="OrthoDB" id="10960at2157"/>
    <dbReference type="PhylomeDB" id="Q979U0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF10">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="8"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="435" mass="47456" checksum="8665AD9BC619CC5A" modified="2001-10-01" version="1">MIQLLNNLWVLFSAFLVFLMTIAVGFLEYGELGIALKRSLYKTILITGIAFVVMAVIGFNTAFAPTLYGIIGNPLYKPGFVLGYLNSPTAMFSTIWWATSASYFNTGLLGITYFLFETAFASVTLALVSVVILRKVKMSVFVVFSIVYFIVIYNLPAAWIWNPTGWLYLLGMRDFAGGLVVHGAAGFAGLAIIIRIWQEERKKGIVRSPGEIIKVNDGFLTLAILLLMMGWFGFNPGSVLAFNYDTLIVVMTTFMAALTSMLSTLTVSYLLYKDAGGIMNAVNGILMGLIIVTPLAGFVSPLSAAILGILGGPIFIFANRFFSKRKWFSDPVGLFSGHFTGGIFGVLMIGFFTQHSYAVLSGNSILPNGLFFGGGYLALHTLGIEIMGICAVGLAVFAISYATMYFLSLGFHGILEEPEWKKEERKSLPSSETSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q979X1</accession>
    <name>Q979X1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1063293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917268.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979X1"/>
    <dbReference type="STRING" id="273116.gene:9381833"/>
    <dbReference type="PaxDb" id="273116-14325277"/>
    <dbReference type="GeneID" id="1441150"/>
    <dbReference type="KEGG" id="tvo:TVG1063293"/>
    <dbReference type="eggNOG" id="arCOG01357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032214_0_0_2"/>
    <dbReference type="OrthoDB" id="358785at2157"/>
    <dbReference type="PhylomeDB" id="Q979X1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF4">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="1">
        <location>
            <begin position="45"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="206"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="540" mass="60602" checksum="648461D84AE7506D" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-16" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q979X5</accession>
    <name>KHSE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Homoserine kinase</fullName>
            <shortName evidence="1">HK</shortName>
            <shortName evidence="1">HSK</shortName>
            <ecNumber evidence="1">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thrB</name>
        <name type="ordered locus">TV1035</name>
        <name type="ORF">TVG1060320</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979X5"/>
    <dbReference type="SMR" id="Q979X5"/>
    <dbReference type="STRING" id="273116.gene:9381828"/>
    <dbReference type="PaxDb" id="273116-14325273"/>
    <dbReference type="KEGG" id="tvo:TVG1060320"/>
    <dbReference type="eggNOG" id="arCOG01027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041243_1_1_2"/>
    <dbReference type="PhylomeDB" id="Q979X5"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002288">
        <property type="entry name" value="PRK01212.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF1">
        <property type="entry name" value="HOMOSERINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00958">
        <property type="entry name" value="HOMSERKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0791">Threonine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156653" description="Homoserine kinase">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00384"/>
        </source>
    </evidence>
    <sequence length="309" mass="32698" checksum="E3E64A828508FD31" modified="2001-10-01" version="1">MPSMELSVISPCSSANLGPGYDVLAIAFDAFYDQVNVMLSDKLRILADEIPVQPDKNTAGLTALKMIEDFGIKDGIKISIKKGIPYGLGLGSSGSSAAAAAYAINNLFALGLERKDLIKYAAVGELASSGSPHPDNVSASILGGLVIVSPEGISAKRIEVSDQFKFLLVIADLKIRDKTKYARSLVPSGVSMGDHKRNTSRVASLIAGLMSGDRELVSTGMNDDIVEAAREPIFPYYRRVKDTAIESGAVGAVVSGAGPSILVVYDNKTETKQMIRKISRIYEGMGISVNFATPNVADGVREVANPLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Y1</accession>
    <name>Q979Y1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1053887 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1053887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        </source>
    </evidence>
    <sequence length="117" mass="13690" checksum="D664FFA8A61211DD" modified="2001-10-01" version="1">MVNQDEPELGELCEKILVHIYRYGPDSPKYMAHRLLGASGWAPTYDEKEIKEKCKKMESLRLIRVFQGPLKRSPTSSVKPWIKIKAREINYKPAGIYYELTKDGKRIAGKLFKEKYR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A64</accession>
    <name>CBIT_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)</fullName>
            <ecNumber evidence="1">2.1.1.196</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiT</name>
        <name type="ordered locus">TV0946</name>
        <name type="ORF">TVG0972073</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylation of C-15 in cobalt-precorrin-6B followed by the decarboxylation of C-12 to form cobalt-precorrin-7.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Co-precorrin-6B + S-adenosyl-L-methionine = Co-precorrin-7 + S-adenosyl-L-homocysteine + CO2</text>
            <dbReference type="Rhea" id="RHEA:36067"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:70791"/>
            <dbReference type="ChEBI" id="CHEBI:72780"/>
            <dbReference type="EC" id="2.1.1.196"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 8/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Archaeal-type CbiT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.196" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917177.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A64"/>
    <dbReference type="SMR" id="Q97A64"/>
    <dbReference type="STRING" id="273116.gene:9381738"/>
    <dbReference type="PaxDb" id="273116-14325163"/>
    <dbReference type="GeneID" id="1442025"/>
    <dbReference type="KEGG" id="tvo:TVG0972073"/>
    <dbReference type="eggNOG" id="arCOG00977">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094143_0_0_2"/>
    <dbReference type="OrthoDB" id="6027at2157"/>
    <dbReference type="PhylomeDB" id="Q97A64"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00229"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043776">
        <property type="term" value="F:cobalt-precorrin-6B C5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00786">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023475">
        <property type="entry name" value="CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050714">
        <property type="entry name" value="Cobalamin_biosynth_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182">
        <property type="entry name" value="COBALT-PRECORRIN-6B C(15)-METHYLTRANSFERASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-7 C(5)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134947" description="Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="52"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00786"/>
        </source>
    </evidence>
    <sequence length="201" mass="21884" checksum="39D5832AA843A49A" modified="2001-10-01" version="1">MESEKSKFDYYIVTPDSLFERVDGIPMTKEEIRLISLNRLGVRNGGHFLDIGTGTGSVAVDMSRLAGPNGKIIALDRDEKAIKLARINLDRLSPYKNIQLVLADAYAYSPADSFDAIFIGGGTGDLPNLVSKYVPFLKSGARVVINAIQVKTLNDAVESLELNNFRNVSVIEVQISVGMKTGSSYAMIARNPIFVVSGEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A85</accession>
    <name>Q97A85_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NDP-sugar epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0954084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917163.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A85"/>
    <dbReference type="STRING" id="273116.gene:9381717"/>
    <dbReference type="PaxDb" id="273116-14325142"/>
    <dbReference type="DNASU" id="1442011"/>
    <dbReference type="GeneID" id="1442011"/>
    <dbReference type="KEGG" id="tvo:TVG0954084"/>
    <dbReference type="eggNOG" id="arCOG04627">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_938945_0_0_2"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="PhylomeDB" id="Q97A85"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="256" mass="29116" checksum="D5ABA978BD500110" modified="2001-10-01" version="1">MMILVTGHRGFIGSHIYSYFNGNSEVYGFDIGDKLEDRRYDVIIHMAARGLIRKSIEFPYDYFQDDLSLVVRFLELARKNDSVFIFPSSGSTAEPTNPYSLAKKNAEEWIRLYGKLYGLKYYILRFFNIYGDGAKKGAVYLFTKAALSGEEAIVYGDGSHIRDFLYVGDVPITIERILNEKYRTGEYEVGSGKGTSVNDLISLIEKVTGKKIRTRHEDYIVPEASELVAKNTIVKNPTPLEVGVERVMEFILKDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A86</accession>
    <name>Q97A86_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0953267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917162.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A86"/>
    <dbReference type="STRING" id="273116.gene:9381716"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325141"/>
    <dbReference type="GeneID" id="1442010"/>
    <dbReference type="KEGG" id="tvo:TVG0953267"/>
    <dbReference type="eggNOG" id="arCOG01397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055387_2_0_2"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="PhylomeDB" id="Q97A86"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="325" mass="37426" checksum="75997BE68BA7A6D8" modified="2001-10-01" version="1">MMANVSVIISAHDRKKYVMNAIKSVLDQSVGRDKIEIILVKNFSDVALDNFCEKNKIKNVLFFGPTLGEKMARGIMESSSEIICFLDDDDMFEPNKVENVLKAFNEDDIVYYHNGVRIIDEDGRIVEKNYTGRMRVNVRLNDARSIKMARKLNGDWYMSCISVRRDFAVSMIDTLKSLPASFDKLMYYSALASKGVLIVDNAPLTRYRLHSSLTTVIGDQDHFLKRKLIFFNKSADTAKYLLNKYGDPKTSSPYRLFYLHELMMLSALGSVNKLSYRETLNALQDIFHYGNRADLIWLILSLFSKVSGKMTSTIVYRYMMNYFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A90</accession>
    <name>Q97A90_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0949121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917158.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A90"/>
    <dbReference type="STRING" id="273116.gene:9381712"/>
    <dbReference type="PaxDb" id="273116-14325137"/>
    <dbReference type="GeneID" id="1442006"/>
    <dbReference type="KEGG" id="tvo:TVG0949121"/>
    <dbReference type="eggNOG" id="arCOG01367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045518_2_1_2"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="PhylomeDB" id="Q97A90"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05254">
        <property type="entry name" value="dTDP_HR_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="DTDP-4-DEHYDRORHAMNOSE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491:SF4">
        <property type="entry name" value="METHIONINE ADENOSYLTRANSFERASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD-like substrate binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="280" mass="31405" checksum="A3F5717064410E99" modified="2001-10-01" version="1">MPNILIFGHTGQLGRELLKLVPNAISGSINGKRVDITDYSEIALLFSQASPDIVINSAAFTNVDRCEKEREKAYLVNAIGVRNIVKLCDKYNSKLIQISTDYVFNGERGNYKEDDIPDPINYYGYTKSIGDAYALSSDNTIVVRTSGVFGNANNFPLFVYNRLKAGLEVDVIKGYYSPIHANLLSKALYVLISKGTHRGIINIAGQRISRFDLATAIANRFGFDQKLIREVPDLKEMVARRPFDSSLDISYAKKLIDFDFYTLESNLNSFERSLKKDGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AA8</accession>
    <name>Q97AA8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dolichol monophosphate mannose synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0922748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917142.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AA8"/>
    <dbReference type="SMR" id="Q97AA8"/>
    <dbReference type="STRING" id="273116.gene:9381694"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325119"/>
    <dbReference type="DNASU" id="1441990"/>
    <dbReference type="GeneID" id="1441990"/>
    <dbReference type="KEGG" id="tvo:TVG0922748"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_9_0_2"/>
    <dbReference type="OrthoDB" id="351177at2157"/>
    <dbReference type="PhylomeDB" id="Q97AA8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="18"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="264" mass="30769" checksum="505A24277056E430" modified="2001-10-01" version="1">MDALVSTKANSIAYQRSTVIIPAYNEEKRLMPVLYELCEYIRSNSLEWKVMVSIDGDDGTESNVKIMMQEYSFLSYSKGKGRGGKGAAIKRAITSATGEFVILMDADGSVPLKEIVKALDLTNYYDLIIFDRYSNRGNRIPFIRRFPSRGFNKLVRIFLGLKINDTQCGYKIIKREYAQRAFNKITISNAFFDVALLYYLKKERAKYIEIPVEYNYDRNSKFHVVELVLGEGISLIAFRIRNSRFYKYVPEKLISLYYRKFRWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AG9</accession>
    <name>Q97AG9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Type-5 uracil-DNA glycosylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG0863458</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 5 (UDGb) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917085.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AG9"/>
    <dbReference type="STRING" id="273116.gene:9381633"/>
    <dbReference type="PaxDb" id="273116-14325058"/>
    <dbReference type="GeneID" id="1441933"/>
    <dbReference type="KEGG" id="tvo:TVG0863458"/>
    <dbReference type="eggNOG" id="arCOG00905">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083279_0_0_2"/>
    <dbReference type="OrthoDB" id="8612at2157"/>
    <dbReference type="PhylomeDB" id="Q97AG9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033958">
        <property type="term" value="F:DNA-deoxyinosine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10031">
        <property type="entry name" value="UDG-F5_TTUDGB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051536">
        <property type="entry name" value="UDG_Type-4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044147">
        <property type="entry name" value="UdgB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693:SF3">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="10">
        <location>
            <begin position="49"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="227" mass="25209" checksum="EC06CCB6609ED1E0" modified="2001-10-01" version="1">MEDVWKDISEMNSDLIGCEKCPRLVTFRKEVAKRDKKFRGQEYWSRPVPGYGDISGRLLIVGLAPAATGGNRTGRVFTGDKSSDFLVSCLFEAGITNQPTSVSRGDGLVYIDSYITAAVKCVPPDNKPTMDEIKNCMPYLIFEVKQMKNLKVVLALGKIAFDSVLDVLRSFGTNTKGMKFVHGNVYDTGTFKLVPSYHPSPRNVNTGKLKREDFVSLLQKVKALISE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AH7</accession>
    <name>SYA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="1">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">alaS</name>
        <name type="ordered locus">TV0833</name>
        <name type="ORF">TVG0850702</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59975" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59975.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_052268466.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AH7"/>
    <dbReference type="SMR" id="Q97AH7"/>
    <dbReference type="STRING" id="273116.gene:9381625"/>
    <dbReference type="PaxDb" id="273116-14325050"/>
    <dbReference type="GeneID" id="1441925"/>
    <dbReference type="KEGG" id="tvo:TVG0850702"/>
    <dbReference type="eggNOG" id="arCOG01255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_4_0_2"/>
    <dbReference type="OrthoDB" id="7506at2157"/>
    <dbReference type="PhylomeDB" id="Q97AH7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000056">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_A">
        <property type="entry name" value="Ala_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022429">
        <property type="entry name" value="Ala-tRNA_lgiase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03683">
        <property type="entry name" value="A-tRNA_syn_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000075280" description="Alanine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="590"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="594"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="694"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="698"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="876" mass="99775" checksum="F54597F617EEA500" modified="2002-08-02" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AI9</accession>
    <name>Q97AI9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">smc</name>
        <name evidence="8" type="ORF">TVG0831149</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMC family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917065.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AI9"/>
    <dbReference type="STRING" id="273116.gene:9381611"/>
    <dbReference type="PaxDb" id="273116-14325037"/>
    <dbReference type="GeneID" id="1441913"/>
    <dbReference type="KEGG" id="tvo:TVG0831149"/>
    <dbReference type="eggNOG" id="arCOG00371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_2_2_2"/>
    <dbReference type="OrthoDB" id="9143at2157"/>
    <dbReference type="PhylomeDB" id="Q97AI9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02169">
        <property type="entry name" value="SMC_prok_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="7">
        <location>
            <begin position="502"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="234"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="658"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="946"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="1141" mass="130109" checksum="D6B6D8C8EA34B6D3" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AM0</accession>
    <name>Q97AM0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0791355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917034.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AM0"/>
    <dbReference type="STRING" id="273116.gene:9381580"/>
    <dbReference type="PaxDb" id="273116-14325006"/>
    <dbReference type="GeneID" id="1441882"/>
    <dbReference type="KEGG" id="tvo:TVG0791355"/>
    <dbReference type="eggNOG" id="arCOG01189">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006533_0_3_2"/>
    <dbReference type="OrthoDB" id="8087at2157"/>
    <dbReference type="PhylomeDB" id="Q97AM0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050154">
        <property type="entry name" value="UbiB_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566">
        <property type="entry name" value="CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566:SF113">
        <property type="entry name" value="PROTEIN ACTIVITY OF BC1 COMPLEX KINASE 7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="112"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="510" mass="59083" checksum="C8FE282642B98698" modified="2001-10-01" version="1">MPSATLKREIHILLKLYPVFRRYLKDRKRAKENDEWDYEIQRNGELAVKAFMDLGPTFIKLGQVLSARPDLMPKEYLAAFRKLQDQVDPDPFPLVKQIIERNLGKIDQVFDEFDENAISGASLGQVYRATYKGRLVVVKVNRFNIEDRLKSDLIAIRRLLKHAKGRIENFLYLSIENVIMDFNKRIFDEIDYRKEVSNMNLIRSNLHEDDRVIIPQVIEGLSSKEVLVMEYISGTKIDNVQILKEKNIDTKELALRVDTVFMRMLLKDTIFHADPHPGNISVADDGSIILYDFGMVGFINDDLRFKLLLLYDGLLNKDSDEIIDALLSLGALSPAANRGIVKRGIDMAIANFYGRTPEDLEIRELLDVANDVIFQFPFRLPRALVLYMRMSSLLEGVCQQLDPDFKFIRVLQKLLYDEGLIQTLYKKRLTNFLDDTLISLEKAVQLVPLLKRKLEYEEEPIKRKDYKIPASIFLGFLLLSFFQMEKIHPITSSILIAIDAILFIYIIIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AM6</accession>
    <name>Q97AM6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Isochorismatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0787823</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917029.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AM6"/>
    <dbReference type="STRING" id="273116.gene:9381574"/>
    <dbReference type="PaxDb" id="273116-14325000"/>
    <dbReference type="GeneID" id="1441877"/>
    <dbReference type="KEGG" id="tvo:TVG0787823"/>
    <dbReference type="eggNOG" id="arCOG01943">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_8_4_2"/>
    <dbReference type="OrthoDB" id="9194at2157"/>
    <dbReference type="PhylomeDB" id="Q97AM6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF6">
        <property type="entry name" value="ISOCHORISMATASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="175" mass="19823" checksum="6DEFFE64061259C7" modified="2001-10-01" version="1">MTKGLVVIDMLNDFVHGTLKTQESLSTVIPARNVLEAFRKKGLPIFFTNDAHYKEDPEMKIWGSHAMKGSWGAQIIEELKPLESEFVIEKHAYSAFYGTNLDQILRARGVTELFLIGLDADICVRHTAADALYRNYAITVVEDAVAARIDKNWKDYFIRVYGANIVKSDEINKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AN6</accession>
    <name>Q97AN6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0779096 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0779096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AN6"/>
    <dbReference type="STRING" id="273116.gene:9381564"/>
    <dbReference type="PaxDb" id="273116-14324990"/>
    <dbReference type="KEGG" id="tvo:TVG0779096"/>
    <dbReference type="HOGENOM" id="CLU_2985890_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="57" mass="6709" checksum="8EACD1FAAE3A02FB" modified="2001-10-01" version="1">MKFYTIREFNVIKNCNAKYTRKIKPIIQRPSKSNIRSIKISSACLDLCQVKYSLPEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-10-11" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AR1</accession>
    <name>NTPTH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside-triphosphatase THEP1</fullName>
            <shortName evidence="1">NTPase THEP1</shortName>
            <ecNumber evidence="1">3.6.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside triphosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV0748</name>
        <name type="ORF">TVG0753265</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has nucleotide phosphatase activity towards ATP, GTP, CTP, TTP and UTP. May hydrolyze nucleoside diphosphates with lower efficiency.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the THEP1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.15" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AR1"/>
    <dbReference type="SMR" id="Q97AR1"/>
    <dbReference type="STRING" id="273116.gene:9381538"/>
    <dbReference type="PaxDb" id="273116-14324964"/>
    <dbReference type="KEGG" id="tvo:TVG0753265"/>
    <dbReference type="eggNOG" id="arCOG01034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103145_1_1_2"/>
    <dbReference type="PhylomeDB" id="Q97AR1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19482">
        <property type="entry name" value="RecA-like_Thep1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00796">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004948">
        <property type="entry name" value="Nuc-triphosphatase_THEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010248">
        <property type="entry name" value="PRK13695.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146:SF1">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03266">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146708" description="Nucleoside-triphosphatase THEP1">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="101"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00796"/>
        </source>
    </evidence>
    <sequence length="178" mass="19779" checksum="958AB3B7946C14F4" modified="2001-10-01" version="1">MAIKIGITGPVGSIKAEALQKIIGMLQNDGLNVQGVLISKVSDDGKLTGYTIEDILTKKKVQFCDEKFVSRVKIDKLGVDTRLLEEILIPSLQKARESADVIIIDEVGKLENTTKKIHSEIEETLKCGKPLIVTLHKRSRNPVLQEIKSLEGVRVFDITPINKNILPFKVMHVLKGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AU7</accession>
    <name>Q97AU7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0720869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053972.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AU7"/>
    <dbReference type="STRING" id="273116.gene:9381501"/>
    <dbReference type="PaxDb" id="273116-14324928"/>
    <dbReference type="GeneID" id="95969401"/>
    <dbReference type="KEGG" id="tvo:TVG0720869"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_4_6_2"/>
    <dbReference type="OrthoDB" id="33505at2157"/>
    <dbReference type="PhylomeDB" id="Q97AU7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021027">
        <property type="entry name" value="Transposase_put_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12323">
        <property type="entry name" value="HTH_OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase putative helix-turn-helix" evidence="1">
        <location>
            <begin position="3"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="123" mass="14683" checksum="0F96459548B3B578" modified="2001-10-01" version="1">MGIDPRNDQAVLIAKHFGSTNFVWNYFLEQRVREYKENDKFLTAFDMMKCLTALKNKYAWLSEVNAQSLQQTLMKLYSTFRSFFKHNTAFPKFKSKKDKQYFIVPQHFKFKGNRLILPNLIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AV3</accession>
    <name>Q97AV3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0710951 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0710951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916965.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AV3"/>
    <dbReference type="STRING" id="273116.gene:9381495"/>
    <dbReference type="PaxDb" id="273116-14324922"/>
    <dbReference type="GeneID" id="1441813"/>
    <dbReference type="KEGG" id="tvo:TVG0710951"/>
    <dbReference type="eggNOG" id="arCOG07393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2353375_0_0_2"/>
    <dbReference type="OrthoDB" id="382271at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="96" mass="10884" checksum="C3137294DBE09785" modified="2001-10-01" version="1">MSFKKDVLDKHNEISSKIDNMEKKLDLLSDNSVIKEYYSEMEKKMNSLSEEVVAGLSEKLDNIANELGKAVEKMESKNQDQTLQAEKKTGKGKTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
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    <name>Q97AY0_THEVO</name>
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        <submittedName>
            <fullName evidence="1">TVG0679253 protein</fullName>
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        <name evidence="1" type="ORF">TVG0679253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="24779907"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="80" mass="9645" checksum="5D609BAA1D42DE50" modified="2001-10-01" version="1">MNTDAIDIFLQQHDLEHLNEDERNNLMQLLKATYPLVILRDRIRTAEMVPNHDRYMRLGYILGHLGYRKEGIWFVYRGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AY4</accession>
    <name>Q97AY4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0674750 protein</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0674750</name>
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    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AY4"/>
    <dbReference type="STRING" id="273116.gene:9381463"/>
    <dbReference type="PaxDb" id="273116-14324891"/>
    <dbReference type="KEGG" id="tvo:TVG0674750"/>
    <dbReference type="HOGENOM" id="CLU_2313949_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="99" mass="11603" checksum="4C5CB4EB8B4C7AE8" modified="2001-10-01" version="1">MRPIQEISDRYNGFNIFRHVETEVRTTYAIRKFNGTVISDIFHRVPKDYSVREFSSNLCYIFHKKTIVKIASNDFAESMSGNYVKISNGKNMYASCYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B06</accession>
    <name>Q97B06_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">O-antigen transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0649573</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916912.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B06"/>
    <dbReference type="STRING" id="273116.gene:9381441"/>
    <dbReference type="PaxDb" id="273116-14324869"/>
    <dbReference type="GeneID" id="1441760"/>
    <dbReference type="KEGG" id="tvo:TVG0649573"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_544693_0_0_2"/>
    <dbReference type="OrthoDB" id="57524at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050833">
        <property type="entry name" value="Poly_Biosynth_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250:SF11">
        <property type="entry name" value="O-ANTIGEN TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250">
        <property type="entry name" value="PST FAMILY PREDICTED COLANIC ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="514" mass="57821" checksum="FA4FFBA35E337F5F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-07-19" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B25</accession>
    <name>GSA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="1">GSA</shortName>
            <ecNumber evidence="1">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="1">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemL</name>
        <name type="ordered locus">TV0634</name>
        <name type="ORF">TVG0626624</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916893.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B25"/>
    <dbReference type="SMR" id="Q97B25"/>
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    <dbReference type="PaxDb" id="273116-14324850"/>
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        <property type="reaction ID" value="UER00317"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
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        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
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        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
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        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PLP-dependent transferases"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000120493" description="Glutamate-1-semialdehyde 2,1-aminomutase">
        <location>
            <begin position="1"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <sequence length="420" mass="46457" checksum="7A763726EBD3E83B" modified="2001-10-01" version="1">MSSKELFELAAKLFPMGVNSPVRYFKDYPFYVKEGKGSTILDVDGNKYIDYCLGYGPLILGHSDPDVTRAVIEQAEKGLLFGEPSENEIKLAEMIKETSKNIEMMRFTNSGTEATMHAIRLARAITGRKLIVKMEGGFHGAHDYSLIKSGSGTLTFGSPSSPGIPDEVAQTVIVGKYNDENNIKEIFQKYGDKIAAVITEPIMGNAGVILPKQGFLEFLRDITQKHGSLLIFDEVITGYRFAFSPFQDIMRIDPDITTMGKIIGGGLPIGLFGGSEEIMKNISPSGNVYEAGTFSGNPMSMAAGYAAMEKLSKQDYSILKKRTQKLVSGIDDILDRKHITHTIKYYGTMFQVFFADHVNNYDDALKARKEVYFKLFKALSKNGVYLPPSQYETNFVSFAHSDSDIDATLAAFEKAVAEMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B44</accession>
    <name>Q97B44_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TVG0609019 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0609019</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916873.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B44"/>
    <dbReference type="STRING" id="273116.gene:9381403"/>
    <dbReference type="PaxDb" id="273116-14324831"/>
    <dbReference type="GeneID" id="1441721"/>
    <dbReference type="KEGG" id="tvo:TVG0609019"/>
    <dbReference type="eggNOG" id="arCOG05326">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_270604_0_0_2"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
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        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="762"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="1202" mass="137089" checksum="D7CB5B5C7651D048" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B47</accession>
    <name>Q97B47_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flagella-related protein H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0601307</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916870.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B47"/>
    <dbReference type="STRING" id="273116.gene:9381400"/>
    <dbReference type="PaxDb" id="273116-14324827"/>
    <dbReference type="GeneID" id="1441718"/>
    <dbReference type="KEGG" id="tvo:TVG0601307"/>
    <dbReference type="eggNOG" id="arCOG04148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094838_1_0_2"/>
    <dbReference type="OrthoDB" id="63735at2157"/>
    <dbReference type="PhylomeDB" id="Q97B47"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006320">
        <property type="entry name" value="PRK08533.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF3">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC-like" evidence="3">
        <location>
            <begin position="11"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="223" mass="25134" checksum="1DB2DBF41E994D20" modified="2001-10-01" version="1">MFFSFKIDGDELDKRMGGGVYVGQLFTILGDNSEGKTLLSLRLAYGFLKNGTSVAYVSSQMPVREFISFSDALGYPVLNEIISGTFMFVTPVFILRKPKKARLDDLLNNEKIREKNVLIIDSLNPAMFYDFDINTYMEKLRKFSENRVVILTASPSSLDDATTLRINQLSTTIVTLHSKELGGMKRHFIDLVKYPMVVRSIQQSIPFRVEPGRGLIVEISSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B74</accession>
    <name>Q97B74_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0571194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916842.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B74"/>
    <dbReference type="STRING" id="273116.gene:9381370"/>
    <dbReference type="PaxDb" id="273116-14324798"/>
    <dbReference type="GeneID" id="1441690"/>
    <dbReference type="KEGG" id="tvo:TVG0571194"/>
    <dbReference type="eggNOG" id="arCOG07377">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055961_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="138"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="401" mass="45681" checksum="1A997C39D308FAF8" modified="2001-10-01" version="1">MTTLKKTVAVAGTVAGGVLLVSLLQPFFLGHITQIDSYFLFSGVDIKYLHFDLLVASIVVVIWVIDLFVSIIDRKELITPKPIESTEEVSHKHAESTFTEVKKWPNQFEYSQAFQNPGYSFARELSGGTVIKNPNVKMTGNMIYSSGNYGLIFKIEKESKYYAIKCFTKGSDLHKRYYEIGKHIKSRNLSCIVNFEYLEAGVRTMKDPSIYYPVLKMDWIEGDSLYTYIKRNLDDGKEIRRIANEFAKSVILLKKNHIAHGDLSSDNIMISNGKVIFVDYDGMYVPSLKDLPSNENGHENFQHPSRRSSDYGEDMDNFSALVIYTSLYVLSLRPEAWKFNGDDPDALLFRKDDFLHYERSEVFDYIKKMGGKSAKLASLLIKSLQDGNVNAVEPTKFYSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B87</accession>
    <name>Q97B87_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aspartate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0557379</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916829.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B87"/>
    <dbReference type="STRING" id="273116.gene:9381356"/>
    <dbReference type="PaxDb" id="273116-14324785"/>
    <dbReference type="GeneID" id="1441087"/>
    <dbReference type="KEGG" id="tvo:TVG0557379"/>
    <dbReference type="eggNOG" id="arCOG00082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_5_2_2"/>
    <dbReference type="OrthoDB" id="35685at2157"/>
    <dbReference type="PhylomeDB" id="Q97B87"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF24">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="8">
        <location>
            <begin position="30"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="380" mass="41401" checksum="2F568605A4691332" modified="2001-10-01" version="1">MLRKLMHVGPSMTSYEVQLEGVRENVGFASPEFLEAMRNSLEGLRIVVGADNSYLPFILPGSGTVAMESVTSILPRKGKVLVASNGVFGNRWKPILDAHRMEYEVLEAEPGKSVDPASIKDKLSSGNFASLVFTHVETSTGVRADIEEICRKARPLVDRIVVDGVASVGGETLKVKEWGIDAIITASQKGLASPPGAALLVVAKDSLNNTAGDGYFFDLKNWVEPMNTYLKGENGYLSTEPVGVVMALSHVFNDIRAEGLENTIKRHRVFSEALNEGLEEIGLKVLADREHWSNTVTAVVAERSDDIVKFALSMGIEFATGVHPKLKGKYFRIGHMGYFNYYDVFSALSVIERSALRAGINIKLGSGLTRAQEILIKNGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-28" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B94</accession>
    <name>RS11_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps11</name>
        <name type="ordered locus">TV0563</name>
        <name type="ORF">TVG0551085</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located on the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59705" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59705.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054090.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B94"/>
    <dbReference type="SMR" id="Q97B94"/>
    <dbReference type="STRING" id="273116.gene:9381348"/>
    <dbReference type="PaxDb" id="273116-14324778"/>
    <dbReference type="KEGG" id="tvo:TVG0551085"/>
    <dbReference type="eggNOG" id="arCOG04240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_6_1_2"/>
    <dbReference type="OrthoDB" id="12054at2157"/>
    <dbReference type="PhylomeDB" id="Q97B94"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044391">
        <property type="term" value="C:ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000018">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019961">
        <property type="entry name" value="Ribosomal_uS11_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03628">
        <property type="entry name" value="arch_S11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000123286" description="Small ribosomal subunit protein uS11">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="132" mass="14122" checksum="F36CEA7873F04F82" modified="2003-02-28" version="2">MEVNRMNKTGIAHIYASQNNTIIHVTDPTGAETIAMVSGGMVVKNDRDQASPYAAMKAADMVSETLKEHEITDLIIKVRAPGGNKSKIPGPGAQAAIRALSRAGLKIVRIEEVTPIPHDGTKKKGGKRGRRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-27" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B96</accession>
    <name>RS13_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps13</name>
        <name type="ordered locus">TV0561</name>
        <name type="ORF">TVG0550096</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916820.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B96"/>
    <dbReference type="SMR" id="Q97B96"/>
    <dbReference type="STRING" id="273116.gene:9381346"/>
    <dbReference type="PaxDb" id="273116-14324776"/>
    <dbReference type="GeneID" id="1441078"/>
    <dbReference type="KEGG" id="tvo:TVG0550096"/>
    <dbReference type="eggNOG" id="arCOG01722">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_0_0_2"/>
    <dbReference type="OrthoDB" id="372127at2157"/>
    <dbReference type="PhylomeDB" id="Q97B96"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019977">
        <property type="entry name" value="Ribosomal_uS13_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003140">
        <property type="entry name" value="PRK04053.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03629">
        <property type="entry name" value="uS13_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000132194" description="Small ribosomal subunit protein uS13">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="171" mass="19515" checksum="6FFDA4C171034D21" modified="2001-10-01" version="1">MGKASANNVKSDKEAAKPAAKKDENKDFQYIVRIANKDLNGERPIPLALSDLKGIGLRLGYAIAERLNLQPTKKIGELKEDEIEKLKEYVENKEYDLPDWLLNHRREPVTGKNLNLVTTDLDVQVQEDINLMKKIRSYKGIRHEKGKKVRGQRTRSNGRRGLSIGVVRKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BD6</accession>
    <name>Q97BD6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0510164 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0510164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_156769055.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BD6"/>
    <dbReference type="STRING" id="273116.gene:9381304"/>
    <dbReference type="PaxDb" id="273116-14324735"/>
    <dbReference type="GeneID" id="1441036"/>
    <dbReference type="KEGG" id="tvo:TVG0510164"/>
    <dbReference type="eggNOG" id="arCOG05391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192650_0_0_2"/>
    <dbReference type="OrthoDB" id="56245at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="83" mass="9869" checksum="BC587A016EBD7DAE" modified="2001-10-01" version="1">MNELINLQEYIENKNVTVKLEYRLNYDAEKICGYIAVYEGDPSDKEDPFEIYKEILDCNLKENDVRKMFERLIKEIDDGSIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BG4</accession>
    <name>Q97BG4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0476992</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916750.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BG4"/>
    <dbReference type="STRING" id="273116.gene:9381273"/>
    <dbReference type="PaxDb" id="273116-14324706"/>
    <dbReference type="GeneID" id="1441008"/>
    <dbReference type="KEGG" id="tvo:TVG0476992"/>
    <dbReference type="eggNOG" id="arCOG01910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024619_1_1_2"/>
    <dbReference type="OrthoDB" id="28112at2157"/>
    <dbReference type="PhylomeDB" id="Q97BG4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07021">
        <property type="entry name" value="Clp_protease_NfeD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052165">
        <property type="entry name" value="Membrane_assoc_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056738">
        <property type="entry name" value="NfeD1b_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YBBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507:SF3">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YBBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25145">
        <property type="entry name" value="NfeD1b_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141322">
        <property type="entry name" value="NfeD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD1b N-terminal" evidence="7">
        <location>
            <begin position="35"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD-like C-terminal" evidence="6">
        <location>
            <begin position="370"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="439" mass="46864" checksum="29D1C541FAD3474B" modified="2001-10-01" version="1">MDSAKALLLIIVIFILSLLLVEGIGNSVATPQKNIVVINLDEEIDAGSANMITSTLSSVSNSTTAAVVIYMNTPGGILENMMQMVSAISSVENQGIITITYVPVDGMAASAGSYVAMACDYIFMGNGSYIGPSTPIVVAGTSLEQQHTTSAMEQYMVSLAEQHGRNTTAVFSMVSNNTAYTDVEAYKIGISNGIYNSLSEALASIHLQNYPLVEVYPSAYDNFLSFIGNAYVDGIFILLGFVAIMLDIYHGSVVLTVIGIALLVLGFLGLQLISASLVGVLLLILGSVLILLEAKMGHGFALLSGVVIGLVGTFMLASPYYSSNPGYSPSPFTTFDLLTSILIVIVAGFLAFYIRRIVRTIKFRGRWTGAESLIGRSAKAVSNINSHGWVSVDGIEWQAKSNDGKPINKGEPVKIVDRSGLVLIVERTNTEEKQDLKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BI2</accession>
    <name>Q97BI2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF72 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0461540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916733.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BI2"/>
    <dbReference type="STRING" id="273116.gene:9381255"/>
    <dbReference type="PaxDb" id="273116-14324688"/>
    <dbReference type="GeneID" id="1440991"/>
    <dbReference type="KEGG" id="tvo:TVG0461540"/>
    <dbReference type="eggNOG" id="arCOG04291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046519_0_1_2"/>
    <dbReference type="OrthoDB" id="35747at2157"/>
    <dbReference type="PhylomeDB" id="Q97BI2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="entry name" value="Protein of unknown function UPF0759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348:SF4">
        <property type="entry name" value="DUF72 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YECE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="TM1631-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="28505" checksum="5EDB5D6F7E952945" modified="2001-10-01" version="1">MVKFFIGCSGWFYLHWKGRFYPEDIHTKYWFRYYSSRFNTVELNSTFYRFPSDKTALGWYNSSPENFTYSLKINRSITHIKRLRDTDEEILKFYNVAGKLKEKLGCLLFQFPPSFKYSEENVSLIAKIPLSKYCRVLEFRHKSWFDKKILSSLVDNGFHIATVSSKDLPFLLIQDVIIYMRMHGDTNGYATDYPLEKLKDMLNRIIDLSPDYAYIYFNNDYNGYAPKNAEEIKSLLLSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BK1</accession>
    <name>Q97BK1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0442721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916714.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BK1"/>
    <dbReference type="STRING" id="273116.gene:9381235"/>
    <dbReference type="PaxDb" id="273116-14324669"/>
    <dbReference type="GeneID" id="1440972"/>
    <dbReference type="KEGG" id="tvo:TVG0442721"/>
    <dbReference type="eggNOG" id="arCOG02316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100860_0_0_2"/>
    <dbReference type="OrthoDB" id="30884at2157"/>
    <dbReference type="PhylomeDB" id="Q97BK1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014424">
        <property type="entry name" value="UCP004897_ACT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004897">
        <property type="entry name" value="UCP004897_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="101"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="171" mass="18870" checksum="6D41E2456D7169B5" modified="2001-10-01" version="1">MWSYIYDKFSRSPSQLKIVKKMISTGIKVTKSFDNEPVLMCGDIEIRPNTLAKAAETDRRSVISVINRIANDDKLYPFFSQLEPVANLWKASVKMGLGVIEIIPESANKPGIIAGISSIIAKHNISIRQVIVDDPEIVEDPKAVVVTDQKVPAELIPELRSVDGVKGITIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BL4</accession>
    <name>Q97BL4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0430785 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0430785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916699.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BL4"/>
    <dbReference type="STRING" id="273116.gene:9381220"/>
    <dbReference type="PaxDb" id="273116-14324656"/>
    <dbReference type="GeneID" id="1440957"/>
    <dbReference type="KEGG" id="tvo:TVG0430785"/>
    <dbReference type="eggNOG" id="arCOG03698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623399_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019254">
        <property type="entry name" value="DUF2250"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017139">
        <property type="entry name" value="UCP037213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10007">
        <property type="entry name" value="DUF2250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037213">
        <property type="entry name" value="UCP037213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="170" mass="19991" checksum="76E8587D96270AAF" modified="2001-10-01" version="1">MIAMEYENEIMDLINDYDMLRVLKHFTVAHVDYAKNITRYTEIPQIRVRAYLERLKEMGLLDYYTNTSIKRTEAKLKKSAEVHKHHTYYQINKKAEAVLKEITPKLYVKVLGKEKLELLCSRKVRKTSPDRCTGLQAMGLIDKCFDLTELGREVFEAALRMQAIRCQDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BL6</accession>
    <name>Q97BL6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Asparagine synthetase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0427721</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916697.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BL6"/>
    <dbReference type="STRING" id="273116.gene:9381218"/>
    <dbReference type="PaxDb" id="273116-14324654"/>
    <dbReference type="DNASU" id="1440955"/>
    <dbReference type="GeneID" id="1440955"/>
    <dbReference type="KEGG" id="tvo:TVG0427721"/>
    <dbReference type="eggNOG" id="arCOG00071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075729_0_0_2"/>
    <dbReference type="OrthoDB" id="8692at2157"/>
    <dbReference type="PhylomeDB" id="Q97BL6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparagine synthetase" evidence="3">
        <location>
            <begin position="26"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Asparagine synthetase" evidence="3">
        <location>
            <begin position="142"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="236" mass="26763" checksum="7448372C7287281F" modified="2001-10-01" version="1">MDYHIQEEKILEVLKENVYGLRGSIAFSGGLDSSLLYVISDRNLVPYTVGYSFSHDVNRAKFVLSLLNGSGKYVTLDEINIENYVKYLKSEYPDINKVEISFELVLEILLENVDSDLLVTGQGADELFFGYKRSVNEMDNSLQMERLLKRTIPREKDMAKRQGKELVTPFLDDRILKLASEISPAYHCLYGGKKALREIAKIAGLPEEVYGYRKKAAQYGSGIDKALKSVSYFGLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BN1</accession>
    <name>RL11_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl11</name>
        <name type="ordered locus">TV0424</name>
        <name type="ORF">TVG0409059</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916681.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BN1"/>
    <dbReference type="SMR" id="Q97BN1"/>
    <dbReference type="STRING" id="273116.gene:9381202"/>
    <dbReference type="PaxDb" id="273116-14324639"/>
    <dbReference type="GeneID" id="1440939"/>
    <dbReference type="KEGG" id="tvo:TVG0409059"/>
    <dbReference type="eggNOG" id="arCOG04372">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_4_0_2"/>
    <dbReference type="OrthoDB" id="8842at2157"/>
    <dbReference type="PhylomeDB" id="Q97BN1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000007">
        <property type="entry name" value="50S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002232">
        <property type="entry name" value="PRK01143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000104451" description="Large ribosomal subunit protein uL11">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="158" mass="16688" checksum="C9B736DF5F280070" modified="2001-10-01" version="1">MAQSVKTMVEGGKATTGPPIGPALGPLGLNVAQVVKEINEKTKEFQGMQVPVTVTVIDPETKKYEITVGVPPTSALLKKELGLEKGASKKKEAVAGNATLEQIKNVAIKKMPSMLAKDLKSAVLEVLGTCVAMGINVEGKDPKEVQKLIKSGQIKIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BR0</accession>
    <name>Q97BR0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0380902 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0380902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916650.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BR0"/>
    <dbReference type="STRING" id="273116.gene:9381172"/>
    <dbReference type="PaxDb" id="273116-14324610"/>
    <dbReference type="DNASU" id="1440908"/>
    <dbReference type="GeneID" id="1440908"/>
    <dbReference type="KEGG" id="tvo:TVG0380902"/>
    <dbReference type="eggNOG" id="arCOG05379">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682781_0_0_2"/>
    <dbReference type="OrthoDB" id="55804at2157"/>
    <dbReference type="PhylomeDB" id="Q97BR0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018632">
        <property type="entry name" value="AAA-associated_dom_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09821">
        <property type="entry name" value="AAA_assoc_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="156" mass="17806" checksum="AD56CED0441117B1" modified="2001-10-01" version="1">MTEVIDPNARIADLVGLLYVMNNIFDGKTDLYQLEKEMEVDIDDLMPIVYTASRLGFVSAESGDIIVTEEGKEYIRASIKRRKEIIREALRNIEPFRTALDLRNFDCETLMENLQRKGVQTFNSPSGKHDLEVILIEWGVYSGLLRKDDDGFSVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BS2</accession>
    <name>Q97BS2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0369160 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0369160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916637.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BS2"/>
    <dbReference type="STRING" id="273116.gene:9381160"/>
    <dbReference type="PaxDb" id="273116-14324598"/>
    <dbReference type="GeneID" id="1440895"/>
    <dbReference type="KEGG" id="tvo:TVG0369160"/>
    <dbReference type="eggNOG" id="arCOG04816">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_432550_0_0_2"/>
    <dbReference type="OrthoDB" id="56714at2157"/>
    <dbReference type="PhylomeDB" id="Q97BS2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01127">
        <property type="entry name" value="TrwB_TraG_TraD_VirD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019476">
        <property type="entry name" value="T4SS_TraD_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10412">
        <property type="entry name" value="TrwB_AAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Type IV secretion system coupling protein TraD DNA-binding" evidence="1">
        <location>
            <begin position="34"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="632" mass="71859" checksum="8B466A3B525C0FD6" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BT3</accession>
    <name>Q97BT3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methylisocitrate lyase</fullName>
            <ecNumber evidence="6">4.1.3.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0361650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate</text>
            <dbReference type="Rhea" id="RHEA:16809"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57429"/>
            <dbReference type="EC" id="4.1.3.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Organic acid metabolism; propanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.30" evidence="6"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BT3"/>
    <dbReference type="STRING" id="273116.gene:9381149"/>
    <dbReference type="PaxDb" id="273116-14324587"/>
    <dbReference type="KEGG" id="tvo:TVG0361650"/>
    <dbReference type="eggNOG" id="arCOG00581">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027389_3_2_2"/>
    <dbReference type="PhylomeDB" id="Q97BT3"/>
    <dbReference type="UniPathway" id="UPA00946"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046421">
        <property type="term" value="F:methylisocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019629">
        <property type="term" value="P:propionate catabolic process, 2-methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000009">
        <property type="entry name" value="2-methylisocitrate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012695">
        <property type="entry name" value="PrpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02317">
        <property type="entry name" value="prpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905:SF5">
        <property type="entry name" value="CARBOXYVINYL-CARBOXYPHOSPHONATE PHOSPHORYLMUTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13714">
        <property type="entry name" value="PEP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="272" mass="30139" checksum="798E519FEBB25485" modified="2001-10-01" version="1">MAPGVVGGIGALIAEKRGFKAAYLSGSQVAGMMGLPDLSVTTMNEVAEEVQRITAISRLPLIVDVDTGFGEVVNVIRTVRVMEASGASAIHMEDQELPKKCGHLNGKKVIDRDEMIRKISAAASARKNEDFMIIARTDARAVNGLEDAIDRANAYLEAGADAIFTEALESKEEFEKMRKQVKGYLLANMTEDGKSPLLSVDDLRSIGYNIVIFPLTAFRTMLKAIDSIYADIKNYGTQRNSLDKIMRRAEFYDLIGYYDYEKEDNVFFEIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BU1</accession>
    <name>Q97BU1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0353785 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0353785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BU1"/>
    <dbReference type="STRING" id="273116.gene:9381141"/>
    <dbReference type="PaxDb" id="273116-14324579"/>
    <dbReference type="KEGG" id="tvo:TVG0353785"/>
    <dbReference type="eggNOG" id="arCOG07408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1173411_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="238" mass="25801" checksum="FCE834B1A1BC87B4" modified="2001-10-01" version="1">MRSKMDDKVFYALVTAVVVIGGIGIGVAYSYTSSHPVTVQATGPYYLTMVVTPNNPYQINATFEHRQPSYFIVGPNNTLESAATINLPAHREIILTIIDYDSGPTPNIGPTGTSNNTTYTKVIGTVGNVEYVYNSTIPVSSYSKNTTQIQKPEAISQIPWYGNSSWGYNITHTFTILSGSTILVNIPSWGGSTTVAYLYLNNTGTYNWQCFVPCGYGNDGWGAAMVTAGWMTGTVNVQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BU9</accession>
    <name>TRUB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="1">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="1">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truB</name>
        <name type="ordered locus">TV0356</name>
        <name type="ORF">TVG0348240</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916610.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BU9"/>
    <dbReference type="SMR" id="Q97BU9"/>
    <dbReference type="STRING" id="273116.gene:9381133"/>
    <dbReference type="PaxDb" id="273116-14324571"/>
    <dbReference type="GeneID" id="1440868"/>
    <dbReference type="KEGG" id="tvo:TVG0348240"/>
    <dbReference type="eggNOG" id="arCOG00987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_3_0_2"/>
    <dbReference type="OrthoDB" id="35866at2157"/>
    <dbReference type="PhylomeDB" id="Q97BU9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07953">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01081">
        <property type="entry name" value="TruB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026326">
        <property type="entry name" value="TruB_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000121974" description="Probable tRNA pseudouridine synthase B">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="211"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="292"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="353" mass="39859" checksum="4898D5B5E290AD69" modified="2001-10-01" version="1">MIEEIQKLNGFIVIDKPQGPTSHQVDYWVRQILGTEKVGHIGTLDPNVTGVLVMAIGKAVRLIDVVHEKPKEYVGVMRFHSDISEEEVREVFRKFTTRIYQLPPVRSAVSRKVRIKTIYELDMIEKKDKIVLFHVKCESGTYIRTLCTDIGYVSGKGGQMVDLRRISTGPFKEDIAITLQDLQAYVDLAKEGKDELFRSHFLDMTYAFIDYPKIVAKKSAVENIAHGSDLYVGGVKLIDGNFQKGDRVCVLSEDNELLGTGIARCDSSNLFMKVVDFDHIFVEAKHGKGDVVRDREKDVQRPGQQVHRNIRDAAHGPDSRTGRGRKETGPQIAPNRVRKLQNKTGVHRRPGSH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BV0</accession>
    <name>KCY_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytidylate kinase</fullName>
            <shortName evidence="1">CK</shortName>
            <ecNumber evidence="1">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine monophosphate kinase</fullName>
            <shortName evidence="1">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cmk</name>
        <name type="ordered locus">TV0355</name>
        <name type="ORF">TVG0347128</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CMP + ATP = CDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dCMP + ATP = dCDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylate kinase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916609.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BV0"/>
    <dbReference type="SMR" id="Q97BV0"/>
    <dbReference type="STRING" id="273116.gene:9381132"/>
    <dbReference type="PaxDb" id="273116-14324570"/>
    <dbReference type="GeneID" id="1440867"/>
    <dbReference type="KEGG" id="tvo:TVG0347128"/>
    <dbReference type="eggNOG" id="arCOG01037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079959_1_0_2"/>
    <dbReference type="OrthoDB" id="31096at2157"/>
    <dbReference type="PhylomeDB" id="Q97BV0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00239">
        <property type="entry name" value="Cytidyl_kinase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011892">
        <property type="entry name" value="Cyt_kin_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02173">
        <property type="entry name" value="cyt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13189">
        <property type="entry name" value="Cytidylate_kin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000132027" description="Cytidylate kinase">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00239"/>
        </source>
    </evidence>
    <sequence length="190" mass="21850" checksum="E273E1A6D969D574" modified="2001-10-01" version="1">MRITIAGKIGSGKSTVSQEISKITGYSVYSSGTFFRESAKKMGMSIEDFNRYAETHPEADYATDGMQKDFMETHDNIIVEGRLAGWICKIYSISAFKVFLYATRYTRLVRFSNREGIEIDEAAKLLDEREISEKKRYLDLYGIDIDDLSIYDIVVNTEFMKPEEVAVLVLNRIDEMSRKEVYTPRILKGM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-02-11" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BW2</accession>
    <name>RS14Z_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S14 type Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps14</name>
        <name type="ordered locus">TV0343</name>
        <name type="ORF">TVG0339762</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 16S rRNA, required for the assembly of 30S particles.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916597.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BW2"/>
    <dbReference type="SMR" id="Q97BW2"/>
    <dbReference type="STRING" id="273116.gene:9381120"/>
    <dbReference type="PaxDb" id="273116-14324558"/>
    <dbReference type="GeneID" id="32154007"/>
    <dbReference type="KEGG" id="tvo:TVG0339762"/>
    <dbReference type="eggNOG" id="arCOG00782">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177289_1_1_2"/>
    <dbReference type="OrthoDB" id="5615at2157"/>
    <dbReference type="PhylomeDB" id="Q97BW2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="entry name" value="30s Ribosomal Protein S14, Chain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_A">
        <property type="entry name" value="Ribosomal_uS14_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023676">
        <property type="entry name" value="Ribosomal_uS14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_uS14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="RIbosomal_uS14_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_uS14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004424">
        <property type="entry name" value="PRK05766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010:SF2">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000131002" description="Small ribosomal subunit protein uS14">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="53" mass="6258" checksum="ABB1E26667CE7E1B" modified="2001-10-01" version="1">MSQVKLQPKKKYGHIDGCVRCGRKRGIVRKYGLHLCRQCFRETARQLGFEKYS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-07-19" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BX2</accession>
    <name>RL22_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl22</name>
        <name type="ordered locus">TV0333</name>
        <name type="ORF">TVG0335848</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054074.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BX2"/>
    <dbReference type="SMR" id="Q97BX2"/>
    <dbReference type="STRING" id="273116.gene:9381110"/>
    <dbReference type="PaxDb" id="273116-14324548"/>
    <dbReference type="GeneID" id="1440845"/>
    <dbReference type="KEGG" id="tvo:TVG0335848"/>
    <dbReference type="eggNOG" id="arCOG04098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_0_2_2"/>
    <dbReference type="OrthoDB" id="314984at2157"/>
    <dbReference type="PhylomeDB" id="Q97BX2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_uL22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057265">
        <property type="entry name" value="Ribosomal_uL22_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003260">
        <property type="entry name" value="PRK04223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125291" description="Large ribosomal subunit protein uL22">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="148" mass="16557" checksum="8F82F3D5192F4D90" modified="2001-10-01" version="1">MKGYSMSVDENNARARIVEADISLKDAVNIAHHIRGMKLDYAKQILEDVVSKKYAIPYFRYLDSVSHRPGKGPGRYPVKAAKVFIDLLSNVENNAEFKGMNTDSLIIKHVAANKGRMIKKYTPKAYGRAGANFKDLINLEVIVTEGDQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-23" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BX3</accession>
    <name>RS19_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small ribosomal subunit protein uS19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>30S ribosomal protein S19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rps19</name>
        <name type="ordered locus">TV0332</name>
        <name type="ORF">TVG0335396</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916586.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BX3"/>
    <dbReference type="SMR" id="Q97BX3"/>
    <dbReference type="STRING" id="273116.gene:9381109"/>
    <dbReference type="PaxDb" id="273116-14324547"/>
    <dbReference type="GeneID" id="1440844"/>
    <dbReference type="KEGG" id="tvo:TVG0335396"/>
    <dbReference type="eggNOG" id="arCOG04099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097347_1_0_2"/>
    <dbReference type="OrthoDB" id="30559at2157"/>
    <dbReference type="PhylomeDB" id="Q97BX3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130018" description="Small ribosomal subunit protein uS19">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="151" mass="17348" checksum="0E70DCAEED8DF548" modified="2001-10-01" version="1">MVVNKQGSVKSIKRKARKSRKVTTGRAKEFSYKGYTLEQLQEMSLEELIKILPARARRSLSREMNHDQKKLVEKLEGDYDDIKTHVRDVIILPSYVGKIVEVYNGNSYSKFEIKPEMIGHYLGEFVMTRKEVKHSGPGVGATRSSKFMPLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BX6</accession>
    <name>RL4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl4</name>
        <name type="ordered locus">TV0329</name>
        <name type="ORF">TVG0333990</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BX6"/>
    <dbReference type="SMR" id="Q97BX6"/>
    <dbReference type="STRING" id="273116.gene:9381106"/>
    <dbReference type="PaxDb" id="273116-14324544"/>
    <dbReference type="KEGG" id="tvo:TVG0333990"/>
    <dbReference type="eggNOG" id="arCOG04071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026535_0_0_2"/>
    <dbReference type="OrthoDB" id="10737at2157"/>
    <dbReference type="PhylomeDB" id="Q97BX6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_A">
        <property type="entry name" value="Ribosomal_uL4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019970">
        <property type="entry name" value="Ribosomall_uL4-arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03672">
        <property type="entry name" value="rpl4p_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000129347" description="Large ribosomal subunit protein uL4">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="255" mass="27651" checksum="3BE6315DE62135BE" modified="2001-10-01" version="1">MNTKVPIYSINGEKRGEIDLPEIFNYSVREDLIRKAFRAISLSLRQPYGSSPLAGLRRVGHTTKPGLGISRMPRIAGGSRVVGIASAVGGKSAHSPRSTKKLYVGINEKERKMAKFSAIAMTASADAVRKRGHRFSESLPLPVVVEDAVSGIKKTKDAVNLLKSIGLYEDILRSKEGKHIRAGRGKMRNRRYKVPKSLLIVGTDSVSLKVFKSLPGVDVASMDSLSIRKLAPGGVGGRLTVYTESAIEKLREANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C01</accession>
    <name>Q97C01_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0313409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916559.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C01"/>
    <dbReference type="SMR" id="Q97C01"/>
    <dbReference type="STRING" id="273116.gene:9381079"/>
    <dbReference type="PaxDb" id="273116-14324519"/>
    <dbReference type="GeneID" id="1440817"/>
    <dbReference type="KEGG" id="tvo:TVG0313409"/>
    <dbReference type="eggNOG" id="arCOG00893">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_985571_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97C01"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011589">
        <property type="entry name" value="UCP004961"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206:SF1">
        <property type="entry name" value="METAL-DEPENDENT HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206">
        <property type="entry name" value="METAL-DEPENDENT HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004961">
        <property type="entry name" value="UCP004961_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="276" mass="31592" checksum="C2D5EEA4174B6B0E" modified="2001-10-01" version="1">MYKGRVFDNHFHLNPGGNYKEAIGRFIKAGGTSINLTNLPLHDRTSGYYENLYEWNIKMSEKIRSEFGIEVVVTIGPYPFDYQFFSNAGLDPINEMRHGLELAAKLVSEEKADAIGEIGYPHIQVDKELMDVYTEMLSEAFEIAGNIDSPVILHTYDLDCKDYERLESLSSSFGKNVKVVKHHARPNDLKCNTHIFRSIGASRKNVIESTALSKKFLLETDFIDDPLYRNKYLPPDSVPKRALMIQQQIENADELLYSIFEELPAHIYGRLKKTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C08</accession>
    <name>Q97C08_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0307259 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0307259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916552.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C08"/>
    <dbReference type="STRING" id="273116.gene:9381071"/>
    <dbReference type="PaxDb" id="273116-14324512"/>
    <dbReference type="GeneID" id="1440810"/>
    <dbReference type="KEGG" id="tvo:TVG0307259"/>
    <dbReference type="eggNOG" id="arCOG07411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2327115_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="105" mass="11249" checksum="06C67D938699F89E" modified="2001-10-01" version="1">MIPWSPWNWEVFLLVILALLALSLIILGILTAYFGSGKSRVAGAAILVIGLVVAVIDIIGPKYLFHFGIGQWAAYVALGTIFYVVAAVIGVVIGLLVFLGFIMKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C22</accession>
    <name>PGP_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglycolate phosphatase</fullName>
            <shortName evidence="1">PGP</shortName>
            <shortName evidence="1">PGPase</shortName>
            <ecNumber evidence="1">3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0283</name>
        <name type="ORF">TVG0294815</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of 2-phosphoglycolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-phosphoglycolate + H2O = glycolate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="EC" id="3.1.3.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal SPP-like hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.18" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916538.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C22"/>
    <dbReference type="SMR" id="Q97C22"/>
    <dbReference type="STRING" id="273116.gene:9381057"/>
    <dbReference type="PaxDb" id="273116-14324497"/>
    <dbReference type="GeneID" id="1440796"/>
    <dbReference type="KEGG" id="tvo:TVG0294815"/>
    <dbReference type="eggNOG" id="arCOG01213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_2_0_2"/>
    <dbReference type="OrthoDB" id="120822at2157"/>
    <dbReference type="PhylomeDB" id="Q97C22"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07514">
        <property type="entry name" value="HAD_Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01419">
        <property type="entry name" value="GPH_hydrolase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006382">
        <property type="entry name" value="PGPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01487">
        <property type="entry name" value="Pglycolate_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002245">
        <property type="entry name" value="PRK01158.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01482">
        <property type="entry name" value="SPP-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000:SF8">
        <property type="entry name" value="HAD SUPERFAMILY HYDROLASE-LIKE, TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01144">
        <property type="entry name" value="C2.B.4:_PGP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00446">
        <property type="entry name" value="phosphoglycolate_phosphatase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146732" description="Phosphoglycolate phosphatase">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01419"/>
        </source>
    </evidence>
    <sequence length="224" mass="24999" checksum="AFB61165D34E9778" modified="2001-10-01" version="1">MIKLVAIDVDGTLTDKDRLISTRAIEFIRKAEKKGIIVSLLSGNVIPVVYALKVFIGINGPVFGENGGVMFDNDGSITKFFSNEKTNSFLDEMSKKTSMRSIFTNKWREASTGFDIDGKDVDYVKAEAEKRGLVVFYSGYSWHLMNKGEDKGFAVKILKEKYGLNYEEILVVGDSNNDMPMFELPVFKACPANATDNVKKASDFVSSYSYGEEIGEVFSHFNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C30</accession>
    <name>Q97C30_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Succinyl-CoA synthetase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0285047</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C30"/>
    <dbReference type="STRING" id="273116.gene:9381048"/>
    <dbReference type="PaxDb" id="273116-14324489"/>
    <dbReference type="KEGG" id="tvo:TVG0285047"/>
    <dbReference type="eggNOG" id="arCOG01337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_4_0_2"/>
    <dbReference type="PhylomeDB" id="Q97C30"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF10">
        <property type="entry name" value="SUCCINATE--COA LIGASE [GDP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="7">
        <location>
            <begin position="28"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="384" mass="42175" checksum="46D34793E81603EF" modified="2001-10-01" version="1">MRVAKNVIITSCYNWSGGVLNLYEYMGKDIFREYGIPVPNGYVVSSPQDVKKFTNPVAVKSQILLGGRGKAGGIKFAKTDEELKNAVSTLLSTKVRNMTVTKVLIEDMLNIKHEYYVSIALNRAAKSPMLIASAMGGMEIENVPDDKIFKRIIDPSLGYSDFIGREASQFMGLPPELSKQFLDILKKLYNVYRGEDCELVEINPLVETGDGKLIAADAKVVIDSDAIYRHREFSIQDPEKTPLELKAEGKGYAFVELDGDIGVIANGAGLTMATLDALLLHKGKPRNFLDLGGTDNVDIVINAFDLVLEAKPKAILVNIFGGVTKCDTVAQGIVEAKKKFDIKIPVVVRLSGVHEVEGRKILQDNGIEAFSEMMPAIERVTKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C38</accession>
    <name>Q97C38_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alcohol dehydrogenase [ADH]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0278316</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916523.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C38"/>
    <dbReference type="STRING" id="273116.gene:9381040"/>
    <dbReference type="PaxDb" id="273116-14324481"/>
    <dbReference type="DNASU" id="1440781"/>
    <dbReference type="GeneID" id="1440781"/>
    <dbReference type="KEGG" id="tvo:TVG0278316"/>
    <dbReference type="eggNOG" id="arCOG01458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_4_2"/>
    <dbReference type="OrthoDB" id="8709at2157"/>
    <dbReference type="PhylomeDB" id="Q97C38"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="6">
        <location>
            <begin position="11"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="328" mass="36174" checksum="EE98DB75846C3864" modified="2001-10-01" version="1">MKAVFVYEPLGNENVKVEEVSEPKKEQGKVIIEVKKAGLNPIDYNVINGKIVYRLNPLPHIPGSEVYGIVKEDSGSFKKGERVIVYNRIFDGTCSQCISGNEHLCENGGIWGVVSNGGYSQLVQIDEKNVFRVPEDASDELAASIGVAALTSFRAIRISGCSPGRSILIYGASGNTGMFAVQLASMMGCDVYAVSRKDWIESFGAIEVFRPDNIPPDMKFDIIINPLGSLFWGDALKHLRTRGILVSFGVLTGREGSIDIADLYTGEKKILGSTGGTREDLRGLLEFMKTHELRVKIAREFKINEIKDALKYYSETHDGRILISMRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C72</accession>
    <name>Q97C72_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-hydroxyisobutyrate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0242719</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916487.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C72"/>
    <dbReference type="STRING" id="273116.gene:9381003"/>
    <dbReference type="PaxDb" id="273116-14324446"/>
    <dbReference type="GeneID" id="1440747"/>
    <dbReference type="KEGG" id="tvo:TVG0242719"/>
    <dbReference type="eggNOG" id="arCOG00247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_0_6_2"/>
    <dbReference type="OrthoDB" id="23890at2157"/>
    <dbReference type="PhylomeDB" id="Q97C72"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981:SF80">
        <property type="entry name" value="BLR4309 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="3">
        <location>
            <begin position="5"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="4">
        <location>
            <begin position="163"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="31088" checksum="64E9E71C937AC619" modified="2001-10-01" version="1">MEKKVVLIGLGIMGYRIAANFKKAGMLNGVFDRKIEKAEAFSKQFGSEFYKDIESAVKSNDVIVTMLYDDSSVIDVYSKAMDYAAGKIFVDMSTISPETSIRVSKDIESKGGEMYDAPVIGTSVVVEKKGLTVLIGGPRDNVGLIEELMGATATSVVYVGPNGSGLYAKLVNNLFLGSYMEALAEAVRFGRSTGLDDNVISSVLIKYSSARSPTSELKVPKMMAGDHSTQFSVKNMLKDLEIIEDTARRKSVPIPSASIALQFYRYLASSGHSEEDISSIYKVLTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C74</accession>
    <name>Q97C74_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0239138</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760297.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C74"/>
    <dbReference type="STRING" id="273116.gene:9381001"/>
    <dbReference type="PaxDb" id="273116-14324444"/>
    <dbReference type="GeneID" id="1440745"/>
    <dbReference type="KEGG" id="tvo:TVG0239138"/>
    <dbReference type="eggNOG" id="arCOG00280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023842_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q97C74"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018538">
        <property type="entry name" value="HerA_barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09378">
        <property type="entry name" value="HAS-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA barrel" evidence="6">
        <location>
            <begin position="8"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase HerA central" evidence="5">
        <location>
            <begin position="129"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="511" mass="57784" checksum="9965E30AC7180100" modified="2001-10-01" version="1">MQGQEYEIGLTVGDNRSDIFTFVISPESQIRKWEYVYIKAGETNLLGRIEDLLSKSDLLNDRMDYESVKKYTTSNIKDNVDICVVKIIGAVKDGIISRSRYLIRPGQPVFRATKEMLESIFKFDDQRSLEIGYLADQPDVKIFANINGLRRHLSIIAQTGAGKSHTAGVLMEELLKKGASIIVLDPHADYVLMKNSDKAIYRDNIKVFRTPMSTGRYTAKLGKVDEFTIKFQDLNDEEVREIMGIHENFVNQSKIVKDVMEKLTGRKDVDEFIQKSEQIDTEKRIAARIKYLKKIKAIFGDKTTDVSEYLAPSRMSVIDLSGLDQSLANYFAYKVISQAYDAKVTGSFEYPVFLFIEEAHNFAPPMKVKGSGVSQMLYDTIKKIAAEGRKFGIFLVIITQRPGKIDQDVLSQCNSQIILRITNPSDQKAILESSENISESLMSDLPSLDVGEAIIVGEIVKMPVIAKIRQRETEEGGLDVDIVEMLQKARMEAEKRNDPRAIVENVKKLME</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C94</accession>
    <name>Q97C94_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alpha-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0218327</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916465.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C94"/>
    <dbReference type="STRING" id="273116.gene:9380979"/>
    <dbReference type="CAZy" id="GH38">
        <property type="family name" value="Glycoside Hydrolase Family 38"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324423"/>
    <dbReference type="GeneID" id="1440725"/>
    <dbReference type="KEGG" id="tvo:TVG0218327"/>
    <dbReference type="eggNOG" id="arCOG03661">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003442_1_0_2"/>
    <dbReference type="OrthoDB" id="35948at2157"/>
    <dbReference type="PhylomeDB" id="Q97C94"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10789">
        <property type="entry name" value="GH38N_AMII_ER_cytosolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017:SF1">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="5">
        <location>
            <begin position="512"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="1013" mass="118484" checksum="C76581440EF4D2DD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CA9</accession>
    <name>Q97CA9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0198518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916449.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CA9"/>
    <dbReference type="STRING" id="273116.gene:9380963"/>
    <dbReference type="PaxDb" id="273116-14324407"/>
    <dbReference type="GeneID" id="1441679"/>
    <dbReference type="KEGG" id="tvo:TVG0198518"/>
    <dbReference type="eggNOG" id="arCOG01773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_14_1_2"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="PhylomeDB" id="Q97CA9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912:SF80">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="44"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="192" mass="22008" checksum="1E3C978A3F3F6C48" modified="2001-10-01" version="1">MGKDVVSFFDENAARYDRWYDEHPREYQEQIKLIKDAIRKGNDVEIGVGTGRFAQKLGIKVGVDPSEGMLRIAAARGIFPILAYADRLPFISKYFDFSLFVVTLCFLEDPVSALKEAKRVSKDVFSAIIEKGSSYADFLEKKKEGFYAYAHFYSERELLDFYSEAGLEAVDSIYSDLETEGMRYRFRVIVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CB8</accession>
    <name>Q97CB8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0189621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916439.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CB8"/>
    <dbReference type="STRING" id="273116.gene:9380954"/>
    <dbReference type="PaxDb" id="273116-14324398"/>
    <dbReference type="DNASU" id="1441669"/>
    <dbReference type="GeneID" id="1441669"/>
    <dbReference type="KEGG" id="tvo:TVG0189621"/>
    <dbReference type="eggNOG" id="arCOG00318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069302_1_0_2"/>
    <dbReference type="OrthoDB" id="40991at2157"/>
    <dbReference type="PhylomeDB" id="Q97CB8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF2">
        <property type="entry name" value="PHOU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="10"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="321" mass="36604" checksum="9DA5F027E7AC294F" modified="2001-10-01" version="1">MGKTTRKVQITGGSTFIVSLPPEWVKRNGITKGSEISISDDGNDLVISSSNPDLPEVSKKIIIPPNYTGKPLQRLLTSSYISGFDTLSIVSKEKMGSELRDDIKRFSKVVMGIEIIEETSKSVVLQNVLNVKTFPLSKAIRRMSLNVGTMIEDTIKAIEDYDEELFQNVIERDDEVDRYQWYIYREVKNHCQYEDSNVFMLVLSRILERMADHTVNICIAMKDRKAVNGRMEMIDNLKFSLDVYNHAVESFYSKNFKVLDGIINQKSEINERKFKLLKILSYDVVLSSIAEEISRIGLYGTDIAELAMDLILSDKLEFSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CD4</accession>
    <name>Q97CD4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0178020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916423.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CD4"/>
    <dbReference type="STRING" id="273116.gene:9380938"/>
    <dbReference type="PaxDb" id="273116-14324382"/>
    <dbReference type="GeneID" id="1441653"/>
    <dbReference type="KEGG" id="tvo:TVG0178020"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_4_2"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="PhylomeDB" id="Q97CD4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="202" mass="21711" checksum="DD03D8EBDBD06637" modified="2001-10-01" version="1">MKINDAVERIDGVVANCYSLKIGDVTVLVDSGTRSGGKKIIDYYERSGGKPDVVVITHHHADHIGGLSEIVERFHPKVYVPDLELKIVSGEEKPPAPKSFIGRIIARSLKFNPVKAIVPASKLDVPGIEVMATPGHTIGSTSYILDDFKLIFSGDAAVESGGKLVINKTFTYEINGAERSLETIASMKGHTVLPGHGNPVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CL8</accession>
    <name>Q97CL8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional activator tenA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0084143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916340.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CL8"/>
    <dbReference type="STRING" id="273116.gene:9380849"/>
    <dbReference type="PaxDb" id="273116-14324297"/>
    <dbReference type="GeneID" id="1441570"/>
    <dbReference type="KEGG" id="tvo:TVG0084143"/>
    <dbReference type="eggNOG" id="arCOG01128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077537_3_0_2"/>
    <dbReference type="OrthoDB" id="85443at2157"/>
    <dbReference type="PhylomeDB" id="Q97CL8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050967">
        <property type="entry name" value="Thiamine_Salvage_TenA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198">
        <property type="entry name" value="BIFUNCTIONAL TH2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198:SF2">
        <property type="entry name" value="SI:CH1073-67J19.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="9"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="205" mass="24105" checksum="EE8AED013B03606F" modified="2001-10-01" version="1">MIAEDLKRRAEDLWEKYVKHEFVERMRDDTLPVDSFKFYLVQDSLYVEEMVRSVIRAAASMPFDMAYEILSKIVLNRDKGMEVHGQLERELGITGKGKMTMTTYSYTRHLIYASTLGWPQFLAAWTPCMWGYSFIGKYVVATKNMYFKRWAEFYASDDYMERVSVILKSLDRFNGDASELSDLFKASVKYEIMFWQSALDNEPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CM2</accession>
    <name>Q97CM2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF131 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0079590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916336.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CM2"/>
    <dbReference type="STRING" id="273116.gene:9380845"/>
    <dbReference type="PaxDb" id="273116-14324293"/>
    <dbReference type="GeneID" id="60596779"/>
    <dbReference type="KEGG" id="tvo:TVG0079590"/>
    <dbReference type="eggNOG" id="arCOG02717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149108_1_0_2"/>
    <dbReference type="OrthoDB" id="383429at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002849">
        <property type="entry name" value="DUF131"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00304">
        <property type="entry name" value="TIGR00304 family membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01998">
        <property type="entry name" value="DUF131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15048" checksum="C92338A5420C0288" modified="2001-10-01" version="1">MNLFRLGYILIIIGVIALVGLAALGQAHFALFVIFPVIYGTGLSVIPFLIIFLGIVIMFFAPFTMGGRYEYKEPQDYVNKMEEEQEEKRKSSFGGLIMIGPIPIIFGNNKTIVYVSIAIAILILLVFILYYFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CT8</accession>
    <name>Q97CT8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA 5'-3' helicase</fullName>
            <ecNumber evidence="15">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">TVG0012073</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="15"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760286.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CT8"/>
    <dbReference type="STRING" id="273116.gene:9380778"/>
    <dbReference type="PaxDb" id="273116-14324227"/>
    <dbReference type="GeneID" id="1441498"/>
    <dbReference type="KEGG" id="tvo:TVG0012073"/>
    <dbReference type="eggNOG" id="arCOG00770">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006515_9_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CT8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.20">
        <property type="entry name" value="Bacterial XPD DNA helicase, FeS cluster domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010643">
        <property type="entry name" value="HBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042493">
        <property type="entry name" value="XPD_DNA_FeS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06777">
        <property type="entry name" value="HBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="7"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAB59155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="627" mass="71940" checksum="215844DEAC8F3D96" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-10-18" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CU4</accession>
    <name>RS19E_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S19e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps19e</name>
        <name type="ordered locus">TV0007</name>
        <name type="ORF">TVG0008143</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in maturation of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916264.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CU4"/>
    <dbReference type="SMR" id="Q97CU4"/>
    <dbReference type="STRING" id="273116.gene:9380772"/>
    <dbReference type="PaxDb" id="273116-14324221"/>
    <dbReference type="DNASU" id="1441492"/>
    <dbReference type="GeneID" id="1441492"/>
    <dbReference type="KEGG" id="tvo:TVG0008143"/>
    <dbReference type="eggNOG" id="arCOG01344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108559_1_0_2"/>
    <dbReference type="OrthoDB" id="371836at2157"/>
    <dbReference type="PhylomeDB" id="Q97CU4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01474">
        <property type="entry name" value="Ribosomal_eS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027548">
        <property type="entry name" value="Ribosomal_eS19_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006811">
        <property type="entry name" value="PRK09333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000153844" description="Small ribosomal subunit protein eS19">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="150" mass="17063" checksum="FD5881CF684EB415" modified="2001-10-18" version="1">MVSVKYVPSDMLINYVSGKLKEEKKIKEPNWVKFVKTGVSKEKPPLQDDWIYVRAASMLRKLYINGYLGISRMSSEYGGKVDRGSKRYHAASGSRSITRFLFHELESAGLVQKTQKGRSLSPQGMSLLDNASKEIIKQLAQKDPLYEKFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-01-11" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>P57719</accession>
    <name>FLA1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Flagellin B1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>41 kDa flagellin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV0607</name>
        <name type="ORF">TVG0598631</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="J. Bacteriol." volume="178" first="902" last="905">
            <title>Isolation and characterization of flagella and flagellin proteins from the Thermoacidophilic archaea Thermoplasma volcanium and Sulfolobus shibatae.</title>
            <authorList>
                <person name="Faguy D.M."/>
                <person name="Bayley D.P."/>
                <person name="Kostyukova A.S."/>
                <person name="Thomas N.A."/>
                <person name="Jarrell K.F."/>
            </authorList>
            <dbReference type="PubMed" id="8550530"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 21-40</scope>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>GLYCOSYLATION</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Glycosylated.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Flagellar filament preparations contain one major band with molecular weight of 41 kDa and several minor higher molecular weight bands; this is the protein sequence of the major band.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916865.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P57719"/>
    <dbReference type="SMR" id="P57719"/>
    <dbReference type="STRING" id="273116.gene:9381395"/>
    <dbReference type="PaxDb" id="273116-14324822"/>
    <dbReference type="GeneID" id="1441713"/>
    <dbReference type="KEGG" id="tvo:TVG0598631"/>
    <dbReference type="eggNOG" id="arCOG01829">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051124_0_1_2"/>
    <dbReference type="OrthoDB" id="102632at2157"/>
    <dbReference type="PhylomeDB" id="P57719"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006325">
        <property type="entry name" value="PRK08541.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903:SF1">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0974">Archaeal flagellum</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="propeptide" id="PRO_0000009419" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000009420" description="Flagellin B1">
        <location>
            <begin position="21"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AA sequence." evidence="2" ref="2">
        <original>ET</original>
        <variation>GI</variation>
        <location>
            <begin position="21"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="8550530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0000305" key="3">
        <source>
            <dbReference type="PubMed" id="8550530"/>
        </source>
    </evidence>
    <sequence length="247" mass="25729" checksum="63CD4B3024C83E22" modified="2001-08-14" version="2" precursor="true">MNKLLRKVRKAFSLKADNKAETGIGTLIVFIAMVLVAAVAATVLVHTAGTLQQKATSTGSQTTQQVSTGIQVNSIYGLDSNKSVPTHGVIEWLAIQISITAGSSPINLANVTISLTYHGVSASLTYVGLENIGNATVTNDVYGFNSAVGGTNNVFNSSYFKTINGASNGSKHFAILVLSDPTNSMTAQYPVISYEDQVDLLVNVSAVFGGITEGQAVSGEVQAPVGSPGVIQFTAPESFVSDVIQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q978A8</accession>
    <name>Q978A8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1563727 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1563727</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917738.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978A8"/>
    <dbReference type="STRING" id="273116.gene:9382323"/>
    <dbReference type="PaxDb" id="273116-14325748"/>
    <dbReference type="GeneID" id="30928332"/>
    <dbReference type="KEGG" id="tvo:TVG1563727"/>
    <dbReference type="HOGENOM" id="CLU_2152756_0_0_2"/>
    <dbReference type="OrthoDB" id="377530at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="111" mass="12483" checksum="2FC2B56146E01C0B" modified="2001-10-01" version="1">MDEDAITFGFVITAVIVFVTGMVWQGLWSLLFAMTISGNLFYETIGIAGLILAFIGALVLLYCALILFVYIVILAVIIGIIALLYLIETRTVKVEHYTITLNPHRRYIIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q978C7</accession>
    <name>Q978C7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Modification methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1542167</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917720.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978C7"/>
    <dbReference type="STRING" id="273116.gene:9382304"/>
    <dbReference type="REBASE" id="6614">
        <property type="entry name" value="M.TvoII"/>
    </dbReference>
    <dbReference type="REBASE" id="685116">
        <property type="entry name" value="M.TvoORF2167P"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325729"/>
    <dbReference type="GeneID" id="1442180"/>
    <dbReference type="KEGG" id="tvo:TVG1542167"/>
    <dbReference type="eggNOG" id="arCOG00108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_10_2_2"/>
    <dbReference type="OrthoDB" id="57527at2157"/>
    <dbReference type="PhylomeDB" id="Q978C7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002295">
        <property type="entry name" value="N4/N6-MTase_EcoPI_Mod-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF24">
        <property type="entry name" value="TYPE III RESTRICTION-MODIFICATION ENZYME STYLTI MOD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00506">
        <property type="entry name" value="D21N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="5">
        <location>
            <begin position="74"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="616" mass="72352" checksum="DBD5E606151FA377" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q978D6</accession>
    <name>Q978D6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Formate hydrogenlyase subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1532638</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917712.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978D6"/>
    <dbReference type="STRING" id="273116.gene:9382295"/>
    <dbReference type="PaxDb" id="273116-14325720"/>
    <dbReference type="GeneID" id="1442172"/>
    <dbReference type="KEGG" id="tvo:TVG1532638"/>
    <dbReference type="eggNOG" id="arCOG01547">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057086_0_0_2"/>
    <dbReference type="OrthoDB" id="43567at2157"/>
    <dbReference type="PhylomeDB" id="Q978D6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052197">
        <property type="entry name" value="ComplexI_49kDa-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43485:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43485">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="3">
        <location>
            <begin position="170"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601501-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="389" mass="43893" checksum="A654E1B9BCBA3335" modified="2001-10-01" version="1">MKYYRFGKPEGRFVGNTEKYSVYEHILSDQRREIPSESYRNLPGEFTFNYGPATGGLIESVAFDFHTPGELIKHVDVYPYIKTRKIKVRGLSPEDALLLVERINGFHAASHAVAFEMAVEDALNIDVPEEVQYSRIIMLELERMRSNLEVVKRLCEPAGFGVPQNQIAYLRENIARIITSFAGHRYFFSSSYVGGCQFNGLVLESVIEDVKEEFEGIFNDLLQSKIFLNRLQNNGKISSSDMLGPAARAAGIEVDARIDEKRLPYSKLGFKPIVFDEPDAFGRFYVRSKEILSSADLISKAIRFSGNSPPAKIEKTNGEGAARVESPQGDLFYYVKINDGKISDVQISSPSLLNIEAFKKSMIGNIFTDYHFNWESFGIWVSELAVVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q978E0</accession>
    <name>Q978E0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rusticyanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1528192</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="6">Binds 1 copper ion per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917708.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978E0"/>
    <dbReference type="PaxDb" id="273116-14325716"/>
    <dbReference type="GeneID" id="1442168"/>
    <dbReference type="KEGG" id="tvo:TVG1528192"/>
    <dbReference type="eggNOG" id="arCOG03700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363709_0_0_2"/>
    <dbReference type="OrthoDB" id="4392at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04231">
        <property type="entry name" value="Rusticyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028871">
        <property type="entry name" value="BlueCu_1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001243">
        <property type="entry name" value="Rusticyanin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03095">
        <property type="entry name" value="rusti_cyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00196">
        <property type="entry name" value="COPPER_BLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Blue (type 1) copper" evidence="7">
        <location>
            <begin position="94"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR03095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="190" mass="21080" checksum="CF9925AC862B5411" modified="2001-10-01" version="1">MDKYKLFALVSLGILISVAAFYGARELASREIGSSGGYITNSELNSLNITPPGVQVSSNQSAIYINNSTTLPVLMGPMYAPSMYSFEILRLINPTIVVKEGVSVHFIVINVDTDSYHNFAISNRGPPYPYMVGMMGLGFEYKAPYLPPVHSDLYAYSEFNYTFSSIGDYWYLCTYPGHAENGMYGEIIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q978E7</accession>
    <name>Q978E7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Fructose-1,6-bisphosphate aldolase/phosphatase</fullName>
            <shortName evidence="15">FBP A/P</shortName>
            <shortName evidence="15">FBP aldolase/phosphatase</shortName>
            <ecNumber evidence="6 15">3.1.3.11</ecNumber>
            <ecNumber evidence="15">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fbp</name>
        <name evidence="16" type="ORF">TVG1520544</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">Homooctamer; dimer of tetramers.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the FBP aldolase/phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="6 15"/>
    <dbReference type="EC" id="4.1.2.13" evidence="15"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978E7"/>
    <dbReference type="STRING" id="273116.gene:9382283"/>
    <dbReference type="PaxDb" id="273116-14325709"/>
    <dbReference type="KEGG" id="tvo:TVG1520544"/>
    <dbReference type="eggNOG" id="arCOG04180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041630_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978E7"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02067">
        <property type="entry name" value="FBP_aldolase_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002803">
        <property type="entry name" value="FBPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036076">
        <property type="entry name" value="FBPase_V_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041126">
        <property type="entry name" value="FBP_aldo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE/PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341:SF1">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01950">
        <property type="entry name" value="FBPase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015647">
        <property type="entry name" value="FBPtase_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111249">
        <property type="entry name" value="Sulfolobus fructose-1,6-bisphosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 15" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton acceptor; for FBP phosphatase activity" evidence="15">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for FBP aldolase activity" evidence="15">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DHAP; for FBP aldolase activity" evidence="15">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="103"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="246"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAB60612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="376" mass="41928" checksum="AFD9D2FCDB19CE54" modified="2001-10-01" version="1">MMKVTISHIKADIGSLPGHSLVDEKVMERIREYIEDHGKNLVSDFRMAHVGDDAQITMVHTKGVDSEEVHELAWNAFKAGTEVAKKLGLYGAGQDLLKDSFSGNLKGMGPGVAEMEITERPSEPFIVYMMDKTEPGAFNLPIYKMFADPFNTPGLVIDETMHEGFRFEIWDVIEHKAIMLEAPSDLYDILAMIGSKSRYVIKRVYTKPAHKKLPDENVAVISTDKLSLIAGKYVGKDDPAAVVRLQSGLPAAGESLEAFAFPYFVSGWMRGSFNGPIMPVSMKDAKMTRFDGPPRVVALGYVLKGGKLEGPVDMFDDPAFDYARRVAGELTDHIRRMGPFEPHRLPDQEMEYTTLPAVVEKLKNRFVDIEKQEVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q978I9</accession>
    <name>Q978I9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type II methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">N-4 cytosine-specific methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1473030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978I9"/>
    <dbReference type="STRING" id="273116.gene:9382235"/>
    <dbReference type="REBASE" id="685105">
        <property type="entry name" value="M.TvoVI"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325665"/>
    <dbReference type="DNASU" id="1442116"/>
    <dbReference type="KEGG" id="tvo:TVG1473030"/>
    <dbReference type="eggNOG" id="arCOG00115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_6_0_2"/>
    <dbReference type="PhylomeDB" id="Q978I9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0680">Restriction system</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="5">
        <location>
            <begin position="28"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="265" mass="30938" checksum="22B0D68BA5642359" modified="2001-10-01" version="1">MNREEVKDSVLLGDSLEIMRRIDDCIYDLIFLDPPYYLQMTPKRLKRWNNRSVPQTVREYWDAFPSFEAYDSFISSVLKEAKRLMSDTSTIWAIGTYHNIFRIGKIMQDMGFWILNDVVWIKTNPMPNWLGVRFTNSTETLIWATKGKEQKNYTYNRNLAKEFGLGKTANNVWVMKTSRGNERVRDENRKSVHPAQKPLELMKRIILSSTKEGDLILDPFAGVGTTGVAASMLGRNFTLIEKDPVYYRAMLSRFSRFGIRYTEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="138" xmlns="https://uniprot.org/uniprot">
    <accession>Q978K1</accession>
    <name>Q978K1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glycine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG1462161</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917646.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978K1"/>
    <dbReference type="STRING" id="273116.gene:9382223"/>
    <dbReference type="PaxDb" id="273116-14325653"/>
    <dbReference type="GeneID" id="1442104"/>
    <dbReference type="KEGG" id="tvo:TVG1462161"/>
    <dbReference type="eggNOG" id="arCOG00405">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015515_2_1_2"/>
    <dbReference type="OrthoDB" id="6113at2157"/>
    <dbReference type="PhylomeDB" id="Q978K1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="5"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB60556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="476" mass="54447" checksum="99C4D8175A74C3FB" modified="2001-10-01" version="1">MSFEDVVELAKRRGFFWPSFSIYGGSSGFYDYGPLGTLLKDNIISIWKKSYAKEGAIFLDTPVIAPADVFKASGHLEKFSDIAVKCEKCKTAYKLENLLKSVGIDVPLNNVEKANAVLEEYDVKCPKCGAPLRGAYEFNLMFKVGTNELYLRPETAQGIFINFKNLYNYSRNTMPIIVCQVGKGFRNEISPRQALIRMREFTQAEVEVFYLNDSDFNPNGNSLKFKLLRNTGEEVTVDLKEALHSGIVKNPVMAYFLEKTYSILSSIGFNPNHLRAREHDPLERAHYSSETWDFEYLMDGEWIEIVGVSDRGTYDLGRHQQFSGENMEVDGNIPRVVEPSYGIDRIVLTILNDGYYIRDNGYKVLKLDPAVSPLHFAVFPLQRRDGLDAKAKELFNKIKQIDPFVYYDEGGNIGRRYARQDEIGTPFCITVDYETLEDSTVTIRERDSTKQVRENIEELLQKISDYPEYPTSLFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q978L5</accession>
    <name>Q978L5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1444617 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1444617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978L5"/>
    <dbReference type="SMR" id="Q978L5"/>
    <dbReference type="STRING" id="273116.gene:9382209"/>
    <dbReference type="PaxDb" id="273116-14325639"/>
    <dbReference type="DNASU" id="1442090"/>
    <dbReference type="KEGG" id="tvo:TVG1444617"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_699464_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978L5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="1">
        <location>
            <begin position="234"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="395" mass="45368" checksum="C1FA13C73EEE6FAA" modified="2001-10-01" version="1">MHVIVKNRYTQEIIHFFLENCRTESLRRSSIYAADFLYGKDQGFFIELRERISNYLDRNVDRISIEIASETGKPFKYIREEILQTIREIKSIRIPEDLHEFVKPGVSALNYNCNDPVLSLFVPLFATILQGSPVLVFANPYNFVSAHIIISMLSEIGLPEGVVNVIPFKPNFNYAYLYGENFSYFQDTYDGLGINRIIQSERHKPATVLWGDIDPDSAVSEFTKRLVRMPFSNEQLLVVDRRMFEYVLNRLKEELSRIVVGDPADGATDLGPVTKDEDLMEALSIVGKYRVERLLAMFRGVEGNLMKPAIILTDDVVKDPFVHAPLVYLVNSASVEDAVNLINEVEADRCALFSDDSKLLKMLYGRTACKEISFNSYMYKDYLFKPFLVKRKILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q978M3</accession>
    <name>Q978M3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1439394</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917625.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978M3"/>
    <dbReference type="STRING" id="273116.gene:9382200"/>
    <dbReference type="PaxDb" id="273116-14325631"/>
    <dbReference type="GeneID" id="1442083"/>
    <dbReference type="KEGG" id="tvo:TVG1439394"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="31298at2157"/>
    <dbReference type="PhylomeDB" id="Q978M3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF18">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="305" mass="34901" checksum="B397E26401C817D0" modified="2001-10-01" version="1">MAEDIIEVEHFKQTYDGKKFVVDDVSFHVKRGEIYGLLGKNGAGKTTTIRTLTTIMPVHYGKVKVLGLDVSTHPEKIRQRIGVVLQSESFDFTTVEKNLKIYGMLWDVPGEVLKARIEEVMEVFDLSRLRKIRALELSGGQKKRLQVAREFLHDMDLLFLDEPTVGLDPIMRRTVLNYIKEKAKAGLTVLYTTQIMEEADYLCDRIAIMNNGKIVAEGTSDFLKSKYGDLKTIKIHISGNVNREDLKMMFPEFVQIEGDEVRLISKNVEDVLPDMILYFKRSGIHIERINVEETSLDDVFLRVVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q978N8</accession>
    <name>HUTH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable histidine ammonia-lyase</fullName>
            <shortName evidence="1">Histidase</shortName>
            <ecNumber evidence="1">4.3.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hutH</name>
        <name type="ordered locus">TV1377</name>
        <name type="ORF">TVG1425265</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-histidine = trans-urocanate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21232"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="EC" id="4.3.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PAL/histidase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.3" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917611.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978N8"/>
    <dbReference type="SMR" id="Q978N8"/>
    <dbReference type="STRING" id="273116.gene:9382185"/>
    <dbReference type="PaxDb" id="273116-14325616"/>
    <dbReference type="GeneID" id="1442069"/>
    <dbReference type="KEGG" id="tvo:TVG1425265"/>
    <dbReference type="eggNOG" id="arCOG04671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014801_4_0_2"/>
    <dbReference type="OrthoDB" id="27422at2157"/>
    <dbReference type="PhylomeDB" id="Q978N8"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00549"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004397">
        <property type="term" value="F:histidine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000005">
        <property type="entry name" value="Histidine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000003">
        <property type="entry name" value="Histidine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005921">
        <property type="entry name" value="HutH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01225">
        <property type="entry name" value="hutH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006871">
        <property type="entry name" value="PRK09367.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0369">Histidine metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000161057" description="Probable histidine ammonia-lyase">
        <location>
            <begin position="1"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="modified residue" description="2,3-didehydroalanine (Ser)" evidence="1">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="cross-link" description="5-imidazolinone (Ala-Gly)" evidence="1">
        <location>
            <begin position="141"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00229"/>
        </source>
    </evidence>
    <sequence length="495" mass="53956" checksum="AB7AB91B06345EEB" modified="2001-10-01" version="1">MIEIDGDNLTLEDVYSVSVLHEPVELSTKARNKVAEIHRKFLDLISSGQTIYGVNTGFGGLLNIKISREEEIELQRNLIRSHSAGVGKYLPTDVVRSIMVIRANTLAKGYSAVSTELIDALLAMINKDVVPAVPEFGSVGASGDLAPLAHIGLAMMGEGQAFLNGELMRSDIALSKVGLKPYEFKEKEGVALINGTSMMAGIMALVTSRAYRTIENAVRSSLLSFEALRGTSKAFSDWIVSARPHLGQIAIAEKMRKYLAGSKNVEKSDKEKVQDAYTLRCIPQVYGAVLDTLEYVSSVLVTEINSATDNPLFNGKEVVSGGNFHGEPIALAADFLSIALTDMGNMIERRIARLVDTNLSGLPPFLVKNSGLNSGYMIPQYTAAALCNRNKVLSYPSSADSIPTSANQEDHVSMGSTSTLKLLEIEENLEYIVAIEFLLGAQALEFSQDPISPVTKAIYTKIREYVKPLDKDRPSYLDIEKIREIMNKNELINAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2020-02-26" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q978S7</accession>
    <name>TREH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trehalase</fullName>
            <ecNumber evidence="1">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Alpha,alpha-trehalase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">TV1338</name>
        <name evidence="4" type="ORF">TVG1381191</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2015" name="Appl. Environ. Microbiol." volume="81" first="4920" last="4931">
            <title>Identification of GH15 family thermophilic archaeal trehalases that function within a narrow acidic-pH range.</title>
            <authorList>
                <person name="Sakaguchi M."/>
                <person name="Shimodaira S."/>
                <person name="Ishida S.N."/>
                <person name="Amemiya M."/>
                <person name="Honda S."/>
                <person name="Sugahara Y."/>
                <person name="Oyama F."/>
                <person name="Kawakita M."/>
            </authorList>
            <dbReference type="PubMed" id="25979886"/>
            <dbReference type="DOI" id="10.1128/aem.00956-15"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF GLU-418 AND GLU-571</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of alpha,alpha-trehalose into two molecules of D-glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by validamycin A.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">48.7 mM for trehalose</KM>
            <text evidence="1">kcat is 63.0 sec(-1).</text>
        </kinetics>
        <phDependence>
            <text evidence="1">Optimum pH is 3.7. Active within a narrow range of acidic pH values (pH 3.3 to 4.0).</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="1">Optimum temperature is 60 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan degradation; trehalose degradation; D-glucose from alpha,alpha-trehalose: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 15 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760278.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978S7"/>
    <dbReference type="SMR" id="Q978S7"/>
    <dbReference type="STRING" id="273116.gene:9382146"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325577"/>
    <dbReference type="GeneID" id="1441455"/>
    <dbReference type="KEGG" id="tvo:TVG1381191"/>
    <dbReference type="eggNOG" id="arCOG03286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010399_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q978S7"/>
    <dbReference type="UniPathway" id="UPA00300">
        <property type="reaction ID" value="UER00535"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045582">
        <property type="entry name" value="Trehalase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF0">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19291">
        <property type="entry name" value="TREH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000448959" description="Trehalase">
        <location>
            <begin position="1"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Less than 1% of wild-type activity." evidence="1">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="418"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="4% of wild-type activity." evidence="1">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="25979886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="25979886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="623" mass="71827" checksum="D2734498519B7DF0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q978T6</accession>
    <name>Q978T6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1372228 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1372228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978T6"/>
    <dbReference type="STRING" id="273116.gene:9382137"/>
    <dbReference type="PaxDb" id="273116-14325568"/>
    <dbReference type="KEGG" id="tvo:TVG1372228"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656982_0_0_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="PhylomeDB" id="Q978T6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="159" mass="17393" checksum="D56A100D853EFB1B" modified="2001-10-01" version="1">MMEEYEILRNTVREFANKEIENISKDIEIKGIPDTLVLKLGAQGFLAATAPQEKGGSGLDELGYIIVLEELSRVSPSVAMKVFLTNFYLQLNPDDDVNYIASGKKRVSVAFPDYYARGKSGKVEVSAGKINATKENVINANVEAIALTDGKDVYAVSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Z6</accession>
    <name>Q978Z6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="2">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1306677</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Z6"/>
    <dbReference type="STRING" id="273116.gene:9382071"/>
    <dbReference type="PaxDb" id="273116-14325505"/>
    <dbReference type="KEGG" id="tvo:TVG1306677"/>
    <dbReference type="eggNOG" id="arCOG01481">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043773_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978Z6"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01571">
        <property type="entry name" value="NAPRTase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053190">
        <property type="entry name" value="NAPRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035809">
        <property type="entry name" value="NAPRTase_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006415">
        <property type="entry name" value="PRK08662.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43202:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43202">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="8">
        <location>
            <begin position="122"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="395" mass="43545" checksum="DB7B682734CAF9EF" modified="2001-10-01" version="1">MGNMKMFNIASEDDIKKGLASDVYFERSISTLTETCKDHRVAMEATVSGPLDTWINFSGLDEVLKLLEGIDVDVFAIPEGTILYPRDSNGIPVPFIRIEGNYCDFGMYETSILGMICQASGISTKSSKVRIAAGDCPFFSFGIRRMHPAVSPMIDRSAFIGGADGVSGILGAKLIGEEPIGTMPHALSIILGDDEAWRLTLKNTKNGQKSVILIDTYMDEKFAAIKVAEEFEHVDYIRLDTPSSRRGNFEALVREVKWELALRGRSDIKIMVSGGLDENTVAKLREAGAQAFGVGTSISSAKPFDFALDIVSIDDKPKTKRGKLSGKKSVLRCEKCRKVEVVPYGVESKPCSCGGVMHNIIVKYMEHGKLLSQYPEPKEIRKRTLSELEYFRSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q979A5</accession>
    <name>Q979A5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1294312</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917491.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979A5"/>
    <dbReference type="STRING" id="273116.gene:9382062"/>
    <dbReference type="PaxDb" id="273116-14325495"/>
    <dbReference type="GeneID" id="1441373"/>
    <dbReference type="KEGG" id="tvo:TVG1294312"/>
    <dbReference type="eggNOG" id="arCOG01340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007415_3_1_2"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="PhylomeDB" id="Q979A5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000020">
        <property type="entry name" value="Protein lysine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014089">
        <property type="entry name" value="AcCoA-synth-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02717">
        <property type="entry name" value="AcCoA-syn-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF1">
        <property type="entry name" value="3-HYDROXYPROPIONATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13549">
        <property type="entry name" value="ATP-grasp_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="7">
        <location>
            <begin position="488"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="693" mass="74410" checksum="FD652611B4FBC1FD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q979C6</accession>
    <name>Q979C6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Oligosaccharyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">TVG1271204</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.</text>
            <dbReference type="EC" id="2.4.99.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.21" evidence="6"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917470.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="STRING" id="273116.gene:9382040"/>
    <dbReference type="PaxDb" id="273116-14325473"/>
    <dbReference type="GeneID" id="1441352"/>
    <dbReference type="KEGG" id="tvo:TVG1271204"/>
    <dbReference type="eggNOG" id="arCOG05365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001590_0_0_2"/>
    <dbReference type="OrthoDB" id="82393at2157"/>
    <dbReference type="BRENDA" id="2.4.99.18">
        <property type="organism ID" value="6326"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="482"/>
            <end position="510"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="2048"/>
            <end position="2071"/>
        </location>
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            <begin position="93"/>
            <end position="200"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00001936"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012602"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BAB60377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="2076" mass="228298" checksum="AF26CD4FDBE638A5" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q979D6</accession>
    <name>Q979D6_THEVO</name>
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        <submittedName>
            <fullName evidence="3">TVG1262323 protein</fullName>
        </submittedName>
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        <name evidence="3" type="ORF">TVG1262323</name>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
            <begin position="39"/>
            <end position="64"/>
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    <feature type="domain" description="SHOCT" evidence="2">
        <location>
            <begin position="82"/>
            <end position="105"/>
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    <evidence type="ECO:0000256" key="1">
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    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="107" mass="12138" checksum="2071C764F77A723F" modified="2001-10-01" version="1">MWILIGLIAVVVVLSLIPIGIYGVGYRGPYFGMMGLPYYGMYFFMPIMAAISILVIILFIYLIAGAFHDGSIMSDENSRATEILKERYAKGEISEEEYRKKLDELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q979E7</accession>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
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            <taxon>Archaea</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60356.1"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ArnR1-like_HTH"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="14"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="107" mass="12691" checksum="C1FD414419B608D3" modified="2001-10-01" version="1">MQRNLNFHFVKRNRERNEIMLDILKIIYTNNGEVGITKLIFSSNTNYKFIKSLTQDLSNAGLIKTYQNKAKHTFTITDKGKDIIERYYNINFLNLRHLQILVDLLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q979G0</accession>
    <name>Q979G0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase</fullName>
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        <name evidence="9" type="ORF">TVG1231223</name>
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    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979G0"/>
    <dbReference type="STRING" id="273116.gene:9382002"/>
    <dbReference type="PaxDb" id="273116-14325439"/>
    <dbReference type="KEGG" id="tvo:TVG1231223"/>
    <dbReference type="eggNOG" id="arCOG04310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="PhylomeDB" id="Q979G0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF37">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="7">
        <location>
            <begin position="126"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="6">
        <location>
            <begin position="234"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="397" mass="43959" checksum="85C52C5CA97FFCBF" modified="2001-10-01" version="1">MSMDFDFTEDQKLLQSTAKDFAEKVIAPNIERMRKDRRIPKEVFDGLAKMGFLGMAIPEKYGGMGMDAVSTGIVAEQIARADPTASIPVLFLVDNAWSYLISKYGNPEVFSGILQKVARGSLITGIGSTESGHGSDVAGIDTVAVKKGDEYIVNGEKSFISLVRDIKEIGGGYVTVVKTDPKAGARGISLLYIPYSEEIEIGYLEEMGREGSSWGTLRFNDLHVPAKYLIGQENRGFNIVHEGFEFARGLISVISASMALKSIENGAAYMKQRKAFGNSIATFEGLQFQLADDVAKMEAALNFAYKALWIYDQEQRYGKYSRFQVSKMIAMAKLISTTWGFDAVNDAMQWQGAYGYSKDCPEEWALRGIRSFQLAEGSREIMKIIIARETLGKEFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q979G1</accession>
    <name>Q979G1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1229743 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1229743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979G1"/>
    <dbReference type="PaxDb" id="273116-14325438"/>
    <dbReference type="KEGG" id="tvo:TVG1229743"/>
    <dbReference type="HOGENOM" id="CLU_2821131_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="66" mass="7786" checksum="7237A16A61E309FB" modified="2001-10-01" version="1">MMNEDHEMVRVDSYIVYICVCYKAITQNIPPSDKLKDIMYEFTGMVNFMLNIIVDKNIISRYSLSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-23" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q979G6</accession>
    <name>PYRDB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit</fullName>
            <shortName>DHOD B</shortName>
            <shortName>DHODase B</shortName>
            <shortName>DHOdehase B</shortName>
            <ecNumber>1.3.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Dihydroorotate oxidase B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Orotate reductase (NADH)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pyrD</name>
        <name type="ordered locus">TV1195</name>
        <name type="ORF">TVG1224769</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of dihydroorotate to orotate with NAD(+) as electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(S)-dihydroorotate + NAD(+) = orotate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.3.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.14"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917428.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979G6"/>
    <dbReference type="SMR" id="Q979G6"/>
    <dbReference type="STRING" id="273116.gene:9381996"/>
    <dbReference type="PaxDb" id="273116-14325433"/>
    <dbReference type="GeneID" id="1441310"/>
    <dbReference type="KEGG" id="tvo:TVG1224769"/>
    <dbReference type="eggNOG" id="arCOG00603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042042_0_0_2"/>
    <dbReference type="OrthoDB" id="36608at2157"/>
    <dbReference type="PhylomeDB" id="Q979G6"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004589">
        <property type="term" value="F:dihydroorotate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002058">
        <property type="term" value="F:uracil binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006212">
        <property type="term" value="P:uracil catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000027">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049622">
        <property type="entry name" value="Dihydroorotate_DH_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005574">
        <property type="entry name" value="PRK07259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43073">
        <property type="entry name" value="DIHYDROPYRIMIDINE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43073:SF2">
        <property type="entry name" value="DIHYDROPYRIMIDINE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148419" description="Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="45"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>FMN</name>
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        <ligand>
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        </location>
        <ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="239"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="261"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="301" mass="32735" checksum="2E825A26FA4AF263" modified="2001-10-01" version="1">MSDISTTLAGIKLVNPFMVASGILDENGYTMKELLERGAAAVVTKSIGISERDGYPTPVIVEYGDSLINAVGLSNPGIENFGEEINIAKEAKRPIIGSVFAYNAEEFTKLSVKMEEYGVDAIELNLSCPHVKGFGLEVGSDPDLVEDIVNEIKSKVKVPVFAKLSPNVSNIIEIAKAAEKADAYVLINTVKAMAIDIYSRSPVLSNLYGGLSGPAIKPVGIRYVYEVKKETGKEIIGVGGISNYKDAIEYIMAGASAVQIGTALYKYGKGIFREMEWQLRTFMDEERFEKIEEMVGVAIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q979H7</accession>
    <name>Q979H7_THEVO</name>
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        <submittedName>
            <fullName evidence="3">TVG1213972 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1213972</name>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmata</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917417.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979H7"/>
    <dbReference type="PaxDb" id="273116-14325422"/>
    <dbReference type="GeneID" id="1441299"/>
    <dbReference type="KEGG" id="tvo:TVG1213972"/>
    <dbReference type="eggNOG" id="arCOG03853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529299_0_0_2"/>
    <dbReference type="OrthoDB" id="56883at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="180" mass="19478" checksum="B91A8E7D040D77A7" modified="2001-10-01" version="1">MAYSTADEGKAKSEIKANTNPYSQEAGVLYTLLFSVLLWWMPVIGPALAGYLGGRKSGSIRAGLISSLTVSGLFVIVSISLLPYKDSILGKVGQYISYGVLPISGSNLVSVSNLATYLYSSVGFAYTFFLLVPSSLMIVVIFSILGGFVSNLRRSETGSEEKLEPVEEDEDVEDNKYDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q979N0</accession>
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        <submittedName>
            <fullName evidence="7">NADH dehydrogenase I chain J</fullName>
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        <name evidence="7" type="ORF">TVG1160017</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979N0"/>
    <dbReference type="STRING" id="273116.gene:9381929"/>
    <dbReference type="PaxDb" id="273116-14325368"/>
    <dbReference type="KEGG" id="tvo:TVG1160017"/>
    <dbReference type="eggNOG" id="arCOG01539">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_828001_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q979N0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829:SF2">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
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        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="314"/>
            <end position="332"/>
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    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="6">
        <location>
            <begin position="3"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="335" mass="37219" checksum="B1D67257C99B6364" modified="2001-10-01" version="1">MVNDVKRILAYSTISQLGYMLAAIGLGPIIGSVAVSLGMFHLVSHAIFKALLFMSAGAFLIAMMDLRDAKQMGGLWKRMPITTTAFFIGALALVAFPGTSGYFSKDPIIYASYNYFTSAPSFAHIWPFLFLMIGSLLTTLYTFRMFFLVATGKPRSVLAERAKDPPVLATIPLMILSLFALILGIWQVPFYKFVAPTLDGVAAAIRVPTAPVYISTLPAILLAIGFFVDLYIYGFEKWKTWDISKTWYYKLVKNKFYIDVLYTRIISERVILPLSAAFSGFENKYNSAVNDLGSDTVTLGSYFRNLQNGVLENYVAVLILAAIVIFIIVEIVELM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-05" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q979S2</accession>
    <name>PCNA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="1">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pcn</name>
        <name type="ordered locus">TV1088</name>
        <name type="ORF">TVG1118724</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PCNA family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60230" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60230.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917320.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979S2"/>
    <dbReference type="SMR" id="Q979S2"/>
    <dbReference type="STRING" id="273116.gene:9381885"/>
    <dbReference type="PaxDb" id="273116-14325326"/>
    <dbReference type="DNASU" id="1441202"/>
    <dbReference type="GeneID" id="1441202"/>
    <dbReference type="KEGG" id="tvo:TVG1118724"/>
    <dbReference type="eggNOG" id="arCOG00488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043978_1_1_2"/>
    <dbReference type="OrthoDB" id="14749at2157"/>
    <dbReference type="PhylomeDB" id="Q979S2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002222">
        <property type="entry name" value="PRK01115.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149222" description="DNA polymerase sliding clamp">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="246" mass="27558" checksum="2AC0DAAB0B27A805" modified="2002-03-05" version="2">MIRMNISVRNLKEITDLLSTIVSEAKFKVDENGMSVTAVDPAHVAMIRLEVPKEVFSEFRSDGTEEIALDIDRLKSVIRLANSSENVGITKDKEKLKFDLGNISKSVSLLDPSTIVTPKIPNIASEYYAIIKRSDFERGLRAAEDISDSIRFVLSSEGFRATSHSESEESEMVLPKDMLSDLSCSDTIKSSYPLEYLLKFIKAVSSADSLKISFRDDYPLSIEFYLDQNPSAKIKGLFLLAPRMEQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q979T0</accession>
    <name>MOAA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable GTP 3',8-cyclase</fullName>
            <ecNumber evidence="1">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaA</name>
        <name type="ordered locus">TV1080</name>
        <name type="ORF">TVG1109522</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917310.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979T0"/>
    <dbReference type="SMR" id="Q979T0"/>
    <dbReference type="STRING" id="273116.gene:9381876"/>
    <dbReference type="PaxDb" id="273116-14325318"/>
    <dbReference type="GeneID" id="1441192"/>
    <dbReference type="KEGG" id="tvo:TVG1109522"/>
    <dbReference type="eggNOG" id="arCOG00930">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_2"/>
    <dbReference type="OrthoDB" id="6925at2157"/>
    <dbReference type="PhylomeDB" id="Q979T0"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_A">
        <property type="entry name" value="MoaA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013485">
        <property type="entry name" value="MoaA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02668">
        <property type="entry name" value="moaA_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001199">
        <property type="entry name" value="PRK00164.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000153031" description="Probable GTP 3',8-cyclase">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="6"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="258"/>
            <end position="260"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="326" mass="37448" checksum="2580B5F456AF49A7" modified="2001-10-01" version="1">MVLTDLYGRPVLSLRIQLNTTCNFNCFFCHMEGTEISGEALKPEEIERVVKIAHKFGVNKIKLTGGEPTLRRDLIDIVKRIRKYITGNISMTTNGVMLPILAYELKKAGLDRVNISMHAFDEDTFQAITGVNSRDRIIKAIDAANEAGLTPVKINFVVLRDLNVDQIPDMIELAAEKHAILQLIEYETTREGESSKEYLKYHMPLDSLEKEIADKALSIERNELHNRPRYIIRTQAGEVKVEFVKPQRNPDFCAHCTRLRITSEGEFKTCLMRSDTNVKFKGINDEKTISELFRQAVLRREPYWRPGDEVRQNEVLAKSIIQNKRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-07-19" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q979T3</accession>
    <name>EF2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor 2</fullName>
            <shortName evidence="1">EF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fusA</name>
        <name type="ordered locus">TV1077</name>
        <name type="ORF">TVG1104527</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917307.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979T3"/>
    <dbReference type="SMR" id="Q979T3"/>
    <dbReference type="STRING" id="273116.gene:9381873"/>
    <dbReference type="PaxDb" id="273116-14325315"/>
    <dbReference type="GeneID" id="1441189"/>
    <dbReference type="KEGG" id="tvo:TVG1104527"/>
    <dbReference type="eggNOG" id="arCOG01559">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_11_1_2"/>
    <dbReference type="OrthoDB" id="6290at2157"/>
    <dbReference type="PhylomeDB" id="Q979T3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01514">
        <property type="entry name" value="Elongation_Factor_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_A">
        <property type="entry name" value="EF_G_EF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004543">
        <property type="entry name" value="Transl_elong_EFG/EF2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00490">
        <property type="entry name" value="aEF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000091051" description="Elongation factor 2">
        <location>
            <begin position="1"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="19"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Diphthamide" evidence="1">
        <location>
            <position position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <sequence length="732" mass="81282" checksum="36071CD725BFACB4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q979T5</accession>
    <name>Q979T5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1102801 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1102801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979T5"/>
    <dbReference type="PaxDb" id="273116-14325313"/>
    <dbReference type="KEGG" id="tvo:TVG1102801"/>
    <dbReference type="eggNOG" id="arCOG07763">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987732_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="137" mass="16119" checksum="E36953816F4A3441" modified="2001-10-01" version="1">MPGNLIIMDNMDMEYPIIEDLLHQYSSFHLLLSDGIVIDEDKINDGARIQRILTVHQLVRILVDQDEDPYIILIRHDVVNGWNNEDIENLYDVMRIKSYYRNCDIYFVVLGGTNIFNFMMLGGFYGKEHTEYETEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q979T8</accession>
    <name>Q979T8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1099635</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979T8"/>
    <dbReference type="STRING" id="273116.gene:9381868"/>
    <dbReference type="PaxDb" id="273116-14325310"/>
    <dbReference type="KEGG" id="tvo:TVG1099635"/>
    <dbReference type="eggNOG" id="arCOG02682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1438185_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q979T8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF1">
        <property type="entry name" value="GH07001P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="188" mass="20340" checksum="E6AD8198020029F5" modified="2001-10-01" version="1">MFASIAWFAYDVSSYGVWNYTPSIFVSTGISVVSSIVSTLLEDLPVIAGFIVCLILIDRIGRRVLQAAGFGLAGISLLTFGLYSMHHTLPFILIFIAFAMMHLFHNIGPTNLTYVYPVEIFPTRVRGTAMGIATSASRIGAILGVFAFPLLIGTFSLSAGLLFFAIFEFLGFIVTVILAPETKATALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q979X8</accession>
    <name>Q979X8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1055578</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917261.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979X8"/>
    <dbReference type="STRING" id="273116.gene:9381825"/>
    <dbReference type="PaxDb" id="273116-14325270"/>
    <dbReference type="GeneID" id="1441143"/>
    <dbReference type="KEGG" id="tvo:TVG1055578"/>
    <dbReference type="eggNOG" id="arCOG00427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042622_0_0_2"/>
    <dbReference type="OrthoDB" id="36101at2157"/>
    <dbReference type="PhylomeDB" id="Q979X8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.30">
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    </dbReference>
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        <property type="entry name" value="DHH_CID"/>
    </dbReference>
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        <property type="entry name" value="DHH_sf"/>
    </dbReference>
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        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051673">
        <property type="entry name" value="SSDNA_exonuclease_RecJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255:SF3">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21763">
        <property type="entry name" value="DHH_CID"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH-CID" evidence="2">
        <location>
            <begin position="215"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="1">
        <location>
            <begin position="378"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="493" mass="54432" checksum="618ACF6F61572F9F" modified="2001-10-01" version="1">MKLQEYFLEKKHRDLYEMAVAAREILRDQEKVVIVSHNDADGLSSSAIAKVTLDRAGISNEVIFVKNLDRDVLKSLPSGFKWFVDLGSGSMHMIRDSGIECLITDHHVPDQFDVDEDHKRDILSFLEFSDFSKIYQVNPHLVGLNGSYDISGSGCTFLVSLAFSEKNLDLVKPAIVGSVGDVQDANSGVLEGLNRVFLRIGQDARKVSVLEDLKFFGRTSRPLKRFLELGSDGIPGISNFYSGAGRLLNFLGIPVKDGEKYRVYNDLSEEEKRKLRTEVVGRFIIAGNNDISKLIGETYIFPDEDKNSPTWDAKEFATLLNSCGRYDQGIVGLMVAAGDRAEFYKKAISLMANHRKNLVNGYNQILSKGLKSLKNVDYFYAGKDINENIVGIIANMVLAKTGETMRPLVTMADSDPGTIKISVRAGKSFLSSGIDLADLFRRAAVKTGGVGGGHDLAAGANIPADRMGDFLKFIDEEIGHSIEVQEKTSRIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A10</accession>
    <name>Q97A10_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG1021365 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1021365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917228.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A10"/>
    <dbReference type="STRING" id="273116.gene:9381793"/>
    <dbReference type="PaxDb" id="273116-14325217"/>
    <dbReference type="DNASU" id="1441110"/>
    <dbReference type="GeneID" id="1441110"/>
    <dbReference type="KEGG" id="tvo:TVG1021365"/>
    <dbReference type="eggNOG" id="arCOG03573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031277_2_0_2"/>
    <dbReference type="OrthoDB" id="146312at2157"/>
    <dbReference type="PhylomeDB" id="Q97A10"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1760.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023098">
        <property type="entry name" value="SerK/SbnI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008323">
        <property type="entry name" value="UCP033563"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36454:SF1">
        <property type="entry name" value="DUF1015 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36454">
        <property type="entry name" value="LMO2823 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06245">
        <property type="entry name" value="DUF1015"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="385" mass="44290" checksum="759E2A8E3EDC6CA4" modified="2001-10-01" version="1">MEVKPFRPLIYRKEIDGMIAPPFDAITPKEEVELKRNKYNITYLTLPKGRDGIPHAKMLLQSWLENGVLIPMKEDVFIVLRQRFYVGDECINRYGLIAKVKVFPESGDVIPHERTFDMYVKERETLMDGISSQLEPIFLVTLKNDLPGYLKAITDGLKEDNTYFGPENVENFVYYINDKRKINKIISLLAEDKSIVADGHHRLQATKNIAARKSGGEREFWSYAMAYITSIHDPGVLVGGVNRVVSNKFRLDMNVLSKYFEINQVERLDETNINLFNGRLYSLKIRIPVYDNLIDASNDFILSKSASMNYTDIENEVIYTHDIYELMSLVNSGSYSFGIILPRWDKMKLIETLLSGRKLPQKSTYFYPKIPSGLAIDTALELRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A11</accession>
    <name>Q97A11_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1020565 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1020565</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A11"/>
    <dbReference type="STRING" id="273116.gene:9381791"/>
    <dbReference type="PaxDb" id="273116-14325216"/>
    <dbReference type="KEGG" id="tvo:TVG1020565"/>
    <dbReference type="eggNOG" id="arCOG05347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1425163_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="190" mass="19996" checksum="248553CE626C8010" modified="2001-10-01" version="1">MMKIKKFLPLLAIAVLLPSVATASVIISNTYTVTTSGHAPGVYLTDGPNYVSANAYKYIYAPVSSGNPKNITSGSTIMVNYTYGSGTDYLLNVLEVNDNDVSGVMYLNGSLPSGISIYISNNSEAYLKGSANSPSLTVTNSTGPMTAYSFGSPINLVSGHTYYISFVIDASSFASGSTGSSSIAINYYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A14</accession>
    <name>Q97A14_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1019998</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917225.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A14"/>
    <dbReference type="STRING" id="273116.gene:9381788"/>
    <dbReference type="PaxDb" id="273116-14325213"/>
    <dbReference type="GeneID" id="1441107"/>
    <dbReference type="KEGG" id="tvo:TVG1019998"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_5_4_2"/>
    <dbReference type="OrthoDB" id="57516at2157"/>
    <dbReference type="PhylomeDB" id="Q97A14"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051598">
        <property type="entry name" value="TSUP/Inactive_protease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN MJ0441-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701:SF5">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="249" mass="26421" checksum="790D81BDDE337DFF" modified="2001-10-01" version="1">MDILEFILLGILVGALTGITGSSGVVAVVPALSIINRYDVATAIGTSLFIDIVTSSIVFIVYLKNKNVNTSIMLPFAIGSIIGSQAGVRIAVSIPSFILKDVFGSFLLLMGVYSVLRRKKNVTSKNSILGGKCKFLLAFSISIIISLATGLMGASGGIMYLIAMLFIFKEDVRVAVGTATALMIISASSGATGYFFYGRIDLIAGIVIGVTSLISGYFFAKLGNKIKRETVNKVLIVMFFTIGIIQIAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A46</accession>
    <name>Q97A46_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0988599</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917194.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A46"/>
    <dbReference type="STRING" id="273116.gene:9381756"/>
    <dbReference type="PaxDb" id="273116-14325181"/>
    <dbReference type="GeneID" id="1442042"/>
    <dbReference type="KEGG" id="tvo:TVG0988599"/>
    <dbReference type="eggNOG" id="arCOG01467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_2_3_2"/>
    <dbReference type="OrthoDB" id="147058at2157"/>
    <dbReference type="PhylomeDB" id="Q97A46"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051328">
        <property type="entry name" value="T7SS_ABC-Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43077:SF10">
        <property type="entry name" value="TRANSPORT PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43077">
        <property type="entry name" value="TRANSPORT PERMEASE YVFS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="20"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="267" mass="29712" checksum="7F041195964CC0E9" modified="2001-10-01" version="1">MGGLLPLTAREIKKWYRNPVYLIVGLLQPVFWIILFGSAFDISKFGGPFVSSFFNGAPDYITYMIGGILTITGLFTGMFSGINIIWDRRLGILQRFLVSPIKRSSIVFSRILASVVRIIVQVIILIAIALVIPNGLRISNSFTVIDALIMVSTVLMISFIFSSIFSIIAIRMTRMETIMGITNMVNLPLMFASYALFPPDLMVSWLSTIAKYNPVSWSAESIRLLIIYGSLNSSQMAQLGTYLLYLFALTVAMIVLVYFVAEGGIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A47</accession>
    <name>Q97A47_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0987795</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ABC transporter superfamily. Drug exporter-1 (DrugE1) (TC 3.A.1.105) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054009.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A47"/>
    <dbReference type="STRING" id="273116.gene:9381755"/>
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    <dbReference type="GeneID" id="1442041"/>
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        <location>
            <begin position="4"/>
            <end position="236"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="329" mass="36761" checksum="438E44A399BF4ADF" modified="2001-10-01" version="1">MPIIKTENLTKIYSGNIKAVSDLNIEIEEAEIYGLLGKNGAGKTTTIKMLTTAIEPTSGNAKVAGYDLLKDREKIRNIIGVVPQDLTTDADLKGIENLRMIAAFYDIPKSVAESRINDLLNIVDLTDWGNKYVSQYSGGMRKRLELICGLVNAPKILFLDEPTLGLDVNTRAKMWKYIRDIQKELGVTIILTSHYLEEVDELANRISIIDHGKILVTGTPDELKSSLKGDIITMQFSGSDEAEAARYYPNALYSSISGEDKVRIKVENSDVELPKILSYLTQKGISPLTINVKKPSLDEVFLEYTGSSIDEDIGPEQFRRMMQNVRRLR</sequence>
</entry>
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    <reference evidence="1 2" key="1">
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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    </evidence>
    <sequence length="110" mass="12422" checksum="D9F86CC09AE00B85" modified="2001-10-01" version="1">MYKKTLFLSILALVAVVLLVYGMIPPISSTPVSEMKEGHSYYIYGEIVYELQSTNRSIFVLYAYGQNITVVYSGPIPAAGSFVLVHGTYKVSLLLQYINADSVYRWYYSL</sequence>
</entry>
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                <person name="Yamamoto Y."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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            <dbReference type="EMBL" id="BAB60027.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="55" mass="6621" checksum="83370250D58964B3" modified="2001-10-01" version="1">MSYTIDAKLKANLLSRYLTKNNIYFIGNEDIKSWLFYNNEKFLRGVKSFFIKYPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AD4</accession>
    <name>Q97AD4_THEVO</name>
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        <recommendedName>
            <fullName evidence="1">Glycosyltransferase 2-like domain-containing protein</fullName>
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                <person name="Yamamoto Y."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
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    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055387_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Glyco_tranf_GTA_type"/>
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    </dbReference>
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        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
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    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="314" mass="36788" checksum="1DD48542F3162F74" modified="2001-10-01" version="1">MGDPFISVIIIAYNRKEFLLDAIKSALDQTLDREYYEIIVIKNFQDDTIDNFIKENNIKGIISENKSLGGKISEALYVSKGEIVSFLEDDDMFDRQKLETVYKIFRRNTNVVYYHNVAEYIDESGKKLGRTNRGLSFNSSSISIRKSVVMHEFLENSKVSVDNMMFYFALESSGKIINGKKVLTLYRYHTSSSHTFAPYRVRLEQTISSYSIDVIHFTELYNYFSKKGTRRAIFNHIMEVSIIANIASRLLGKNQPYRISFSQFLRWMSILYYWDSPIAYPLKYIRVFELFGPVPIVYKVQKYLMRDSIKEDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AF4</accession>
    <name>Q97AF4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0877087</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917100.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AF4"/>
    <dbReference type="STRING" id="273116.gene:9381648"/>
    <dbReference type="PaxDb" id="273116-14325073"/>
    <dbReference type="DNASU" id="1441948"/>
    <dbReference type="GeneID" id="1441948"/>
    <dbReference type="KEGG" id="tvo:TVG0877087"/>
    <dbReference type="eggNOG" id="arCOG00683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040997_0_0_2"/>
    <dbReference type="OrthoDB" id="295419at2157"/>
    <dbReference type="PhylomeDB" id="Q97AF4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="171"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="301"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="50405" checksum="2541B1147156FB08" modified="2001-10-01" version="1">MKRANAVKLIVGKETHEKLKELAIVAAKCWNEVNWLRMQQFKEGERVDFSKTEKEVYEKYKQILKVNTQQVARKNAESWRSFFSLIEEKKGKLPKWFKPRPPGYWKDKSGKYKMLIIIRNDRYEIDEEKRIIYLKDFKLSLSFNGKLKWRGKQGRLEIIYNEARRSWYAYIPVEVQNDVKAEDKLKASIDLGIINLATVYVEDGSWYIFKGGSVLSQYEYYSKRISVAQKTLARHKQGRSREMKLLHEKRKRFLKHALNSMVRKIMEEFKNKGVGEIAIGYPKEISKDHGNKLTVNFWNYGYIIRRFEGVGEELGVKVVKVDEAWTSKTCSLCGEAHDDGRIKRGLYRCLRIGKVINADLNGAINILHIPESLGAGSRGQLTVRDRGNGLKTQPAVYRWTNGAGWVSSPTSYEVMKMKAVNCKPMNRHKGTFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AI0</accession>
    <name>Q97AI0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NADH peroxidase/oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0846070</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917074.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AI0"/>
    <dbReference type="STRING" id="273116.gene:9381622"/>
    <dbReference type="PaxDb" id="273116-14325047"/>
    <dbReference type="DNASU" id="1441922"/>
    <dbReference type="GeneID" id="1441922"/>
    <dbReference type="KEGG" id="tvo:TVG0846070"/>
    <dbReference type="eggNOG" id="arCOG01069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_1_3_2"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="PhylomeDB" id="Q97AI0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF1">
        <property type="entry name" value="NAD(P)H SULFUR OXIDOREDUCTASE (COA-DEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="7">
        <location>
            <begin position="319"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="435" mass="46941" checksum="4F2448612CC194AB" modified="2001-10-01" version="1">MKIIVIGGGAAGMSAASKAKRVNPDSEVIVVESGSYVSYAECGIPYYLQGVVGHIEDLLHYPLSEFTEKRGIKVLLHTTVTRIDTLHSSVLLNNGDVLKYDRLIIATGSRPKPIDVISDRTISIRSLESAMRARNLIEKSKDVTIVGAGVLGVELASVLAESGKNVKVISKYDRVLPQLDEDIGKIFHEYFSSRIDVQKQSSLKSAEEDRGRVKITTSVDDHECDAVIYATGIIPNSEIAVEAGIKTGDRNAILTNGHMETSEPGVYAAGDVATVRNIVTGLDEMMPLAQIANKAGRVAGSNASNSEMVFPGALGTTLVKVFDYEVGFTGINESRAKALGIPYEKTLIKAKSRANYYPGKQDIYVKIIYGKNDKRLLGAQVIGTDGAAWRLNTIATAIFGGFKVSDLFYDDLGYTPPFGPVWDSIVIAGSVSMRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AI2</accession>
    <name>HELS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase Hel308</fullName>
            <ecNumber evidence="1">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA 3'-5' helicase Hel308</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hel308</name>
        <name type="ordered locus">TV0828</name>
        <name type="ORF">TVG0843689</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. Hel308 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917072.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AI2"/>
    <dbReference type="SMR" id="Q97AI2"/>
    <dbReference type="STRING" id="273116.gene:9381620"/>
    <dbReference type="PaxDb" id="273116-14325044"/>
    <dbReference type="GeneID" id="1441920"/>
    <dbReference type="KEGG" id="tvo:TVG0843689"/>
    <dbReference type="eggNOG" id="arCOG00553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006553_3_0_2"/>
    <dbReference type="OrthoDB" id="371946at2157"/>
    <dbReference type="PhylomeDB" id="Q97AI2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00442">
        <property type="entry name" value="Helicase_Hel308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048772">
        <property type="entry name" value="Hel308-like_dom4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022965">
        <property type="entry name" value="Helicase_Hel308"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046931">
        <property type="entry name" value="HTH_61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002251">
        <property type="entry name" value="PRK01172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF10">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HEL308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21280">
        <property type="entry name" value="Helicase_dom4_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20470">
        <property type="entry name" value="HTH_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000102115" description="ATP-dependent DNA helicase Hel308">
        <location>
            <begin position="1"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="31"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="1">
        <location>
            <begin position="224"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box" evidence="1">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="44"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00442"/>
        </source>
    </evidence>
    <sequence length="674" mass="76594" checksum="B008EDD1F63FEC84" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AI7</accession>
    <name>Q97AI7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF998 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0831893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917067.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AI7"/>
    <dbReference type="PaxDb" id="273116-14325039"/>
    <dbReference type="GeneID" id="1441915"/>
    <dbReference type="KEGG" id="tvo:TVG0831893"/>
    <dbReference type="eggNOG" id="arCOG02008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1381488_0_0_2"/>
    <dbReference type="OrthoDB" id="46160at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42241">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF998 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42241:SF2">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF998 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="21962" checksum="170A446FC8500F8C" modified="2001-10-01" version="1">MASNKYISYFSLAGIVGPIFAISLVFIDVAVSPWFTWQHNALSDLGVHPYGYLFNLGIIVEGFLNVLFVMALFEIGLKKRIGALLIISGIALALVGVFNEDHPPYHLIFALIYFLLFPIGIIVASIPRLFPFYVKLYSMLASILSLIDIFIGIGFVFKFISIKNVGLSIPEIIEALLLGSWSVLVSVYAFRKLRINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AL6</accession>
    <name>Q97AL6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0794961 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0794961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AL6"/>
    <dbReference type="STRING" id="273116.gene:9381584"/>
    <dbReference type="PaxDb" id="273116-14325010"/>
    <dbReference type="KEGG" id="tvo:TVG0794961"/>
    <dbReference type="HOGENOM" id="CLU_1976653_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="126" mass="13506" checksum="7D4ED44CFD2C2084" modified="2001-10-01" version="1">MASFLVNFPDAVAASSILSIRGLSFDAENRSFSLTVKSSGTTIPDVLKFGANLSPFLTRRFSLTASSTNLLTVSGASNFLLKDITFMKSWRPNDSTFLVSAATMAISFLAASLSIFLYVGVTKKDC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AP1</accession>
    <name>CDC6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cdc6</name>
        <name type="ordered locus">TV0769</name>
        <name type="ORF">TVG0773033</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59911" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59911.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917016.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AP1"/>
    <dbReference type="SMR" id="Q97AP1"/>
    <dbReference type="STRING" id="273116.gene:9381559"/>
    <dbReference type="PaxDb" id="273116-14324985"/>
    <dbReference type="DNASU" id="1441864"/>
    <dbReference type="GeneID" id="1441864"/>
    <dbReference type="KEGG" id="tvo:TVG0773033"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_3_1_2"/>
    <dbReference type="OrthoDB" id="195574at2157"/>
    <dbReference type="PhylomeDB" id="Q97AP1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000073">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001625">
        <property type="entry name" value="PRK00411.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF22">
        <property type="entry name" value="ORC1-TYPE DNA REPLICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151025" description="ORC1-type DNA replication protein">
        <location>
            <begin position="1"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="64"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="404" mass="45905" checksum="872F160DF97822AE" modified="2002-09-19" version="2">MDNPFSKFTNSRTIQADLSLLEENYIPDSFPHREKQINDMVTILSSIMRNSRPSNIIVYGKTGTGKTSTTKYVTRMLQEATSNVEVIYVNCEIYDSPYSILVYVANFAGGEKIPELGWPVDRIYREAVDRINGTGKFFVIVLDEMDRLIKKSGGDSLYVLLKLMTDVENVKVSMIGITNDTTLLENLDSRIKSRLNQESIVFPPYNATEIRDIIASRLEKVFGPGVVDDTAINLCAAIGAQEHGDARKAIDLMRIAIEIAIRENRLKVTEKEVYEARDKYEMNVMHEAINTLPIHSKIVLLSAIVTQEIEPNSVITGEIYENYRRICEDLGFTPLTPRRISDLLTELSDYGLIVVDERNMGKYGRTRGFSVVKEIETIKKYLMEDENLSMFKTSKMTKQARFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AP4</accession>
    <name>Q97AP4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0769512 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0769512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917013.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AP4"/>
    <dbReference type="STRING" id="273116.gene:9381556"/>
    <dbReference type="PaxDb" id="273116-14324982"/>
    <dbReference type="GeneID" id="1441861"/>
    <dbReference type="KEGG" id="tvo:TVG0769512"/>
    <dbReference type="eggNOG" id="arCOG05278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200908_0_0_2"/>
    <dbReference type="OrthoDB" id="9023at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009409">
        <property type="entry name" value="DUF1059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06348">
        <property type="entry name" value="DUF1059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="59" mass="7182" checksum="9BBD6B10CB6C9301" modified="2001-10-01" version="1">MSYVFRCRDYGFDCSYTLEEERKEDIPPMVKMHLRYAHGIYDFTDEVRDKVLSVIKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AP8</accession>
    <name>Q97AP8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0766549 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0766549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="journal article" date="2010" name="Protein Sci." volume="19" first="808" last="814">
            <title>A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Walton T.A."/>
                <person name="Rees D.C."/>
            </authorList>
            <dbReference type="PubMed" id="20162616"/>
            <dbReference type="DOI" id="10.1002/pro.360"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="3LFK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A/B/C/D=1-126"/>
    </dbReference>
    <dbReference type="PDBsum" id="3LFK"/>
    <dbReference type="AlphaFoldDB" id="Q97AP8"/>
    <dbReference type="SMR" id="Q97AP8"/>
    <dbReference type="PaxDb" id="273116-14324978"/>
    <dbReference type="KEGG" id="tvo:TVG0766549"/>
    <dbReference type="HOGENOM" id="CLU_2165381_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055224">
        <property type="entry name" value="MLPTv-like_wHTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22393">
        <property type="entry name" value="MLPTv-like_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MLPTv-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="21"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3LFK"/>
        </source>
    </evidence>
    <sequence length="126" mass="14280" checksum="258CB7604E26714A" modified="2001-10-01" version="1">MSAMAESKVLVKGTPFNKPVIKGKLENNYDMSQDEVSLLLFLKTHGGKIPLYRIKNETGLKDPESVLKNLMDYGFALEDKERLGEKIVLTSEGEFVAQAIRVRDEELRLKEMKQKKNVNRSSAPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AQ3</accession>
    <name>Q97AQ3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Succinate dehydrogenase cytochrome b subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0760633</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917003.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AQ3"/>
    <dbReference type="STRING" id="273116.gene:9381546"/>
    <dbReference type="PaxDb" id="273116-14324972"/>
    <dbReference type="GeneID" id="1441851"/>
    <dbReference type="KEGG" id="tvo:TVG0760633"/>
    <dbReference type="eggNOG" id="arCOG02244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127125_2_1_2"/>
    <dbReference type="PhylomeDB" id="Q97AQ3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="140" mass="16128" checksum="B727874DBAE8553A" modified="2001-10-01" version="1">MVEANKEIREGIWSWAKFYRKGWDYFAFTMHRISGIVILFYLYLHYIVLSNLLHPGPNGIDYNKIVTSITVGPYDSFLVLDFLLALVIFYHGANGVRLALNEFGIGLNKNKALFIIFEVISMILLGLFDYYALQFAGVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AQ9</accession>
    <name>Q97AQ9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="13">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">TVG0754205</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="13"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916997.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AQ9"/>
    <dbReference type="STRING" id="273116.gene:9381540"/>
    <dbReference type="PaxDb" id="273116-14324966"/>
    <dbReference type="GeneID" id="1441845"/>
    <dbReference type="KEGG" id="tvo:TVG0754205"/>
    <dbReference type="eggNOG" id="arCOG00798">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_334840_0_0_2"/>
    <dbReference type="OrthoDB" id="56847at2157"/>
    <dbReference type="PhylomeDB" id="Q97AQ9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.486.10">
        <property type="entry name" value="PCRA, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF67">
        <property type="entry name" value="DNA 3'-5' HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 15 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="16">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="17">
        <location>
            <begin position="279"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAB59892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="885" mass="101878" checksum="CE3DB79E22B20D2E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AR3</accession>
    <name>Q97AR3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4-hyroxybenzoate octaprenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0751384</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916993.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AR3"/>
    <dbReference type="STRING" id="273116.gene:9381536"/>
    <dbReference type="PaxDb" id="273116-14324962"/>
    <dbReference type="GeneID" id="1441841"/>
    <dbReference type="KEGG" id="tvo:TVG0751384"/>
    <dbReference type="eggNOG" id="arCOG00476">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073311_1_1_2"/>
    <dbReference type="OrthoDB" id="11851at2157"/>
    <dbReference type="PhylomeDB" id="Q97AR3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13961">
        <property type="entry name" value="PT_UbiA_DGGGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050475">
        <property type="entry name" value="Prenyltransferase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723:SF1">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="269" mass="29257" checksum="B544E38EFBEAA8F2" modified="2001-10-01" version="1">MSRFSIVRPVNGFMGFISTYISAFIAIGYAVTSHLLPVTLAAFSVFLVTSGGNIINDIVDIDVDRVNHPNRPLVTGEIRVSSAKFIASAMFAVAIVLSLLISFIATGIVVLAIMLLIFYELYFKKTGLPGNTVISLLIGLIFVFGGISVSSYGKMIFLFVLAFTSNMSREIIKDVEDVNGDKDRITFPKKYGVKKALLVSDIIIGVLILVSFIPYLLKILSIYYLYVVIVADAIFILSGIFSSRNPSIGQKVSKYAMIVGMASFLAGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AS2</accession>
    <name>Q97AS2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carboxylesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0742213</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AS2"/>
    <dbReference type="SMR" id="Q97AS2"/>
    <dbReference type="STRING" id="273116.gene:9381527"/>
    <dbReference type="ESTHER" id="thevo-TVG0742213">
        <property type="family name" value="Hormone-sensitive_lipase_like"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324953"/>
    <dbReference type="KEGG" id="tvo:TVG0742213"/>
    <dbReference type="eggNOG" id="arCOG02638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_4_2"/>
    <dbReference type="PhylomeDB" id="Q97AS2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="121"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="351" mass="38725" checksum="F5029A112544F1C3" modified="2001-10-01" version="1">MNPGCRLRGPMGYPDYLTGPLSLSCINIKTKRNIQKAYFRMFDVNPMPIDPQVRKYLDAMSKVSVPELNKTPIEDLRKIFDTPASGAPKVKISVVEDMILDTDAAKLPARLYDDSNSDSLVLYFHGGGFVFGSIETHDSLCRRIAKYSGSKVISVGYRLGPENKFPVAQIDAFNSYLYVLKRSEELGVDSDKIALMGDSAGGNLAAVASLMARDKGIKLPRLQVLVYPSTGPDMTSKSYLEYSSIYLTKELLNWFSSQYLNSIEDALNPYFSPILADLSGLPEAIVVTDEYDPLLEQGETYSATLRDSGVPVTSIRVNGMIHGFLSNFHLFRAADVSLMMISGAIHTILES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AT7</accession>
    <name>Q97AT7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0730458 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0730458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AT7"/>
    <dbReference type="STRING" id="273116.gene:9381511"/>
    <dbReference type="PaxDb" id="273116-14324938"/>
    <dbReference type="KEGG" id="tvo:TVG0730458"/>
    <dbReference type="HOGENOM" id="CLU_1912452_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="132" mass="15257" checksum="981F493AD2FB09D0" modified="2001-10-01" version="1">MLQKLYKIGSITLDIMYPTSIDKRGKLDYFFYRFLLSKGLTSITTMAFIIYFMWIIVVRSHSVFLYGLIITIYLAVQIVFSIPIGHMIDRINNTALNFLSSFIIVISYSLLLLNDSITAIYRNCGINFWANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AU3</accession>
    <name>Q97AU3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0723097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916974.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AU3"/>
    <dbReference type="STRING" id="273116.gene:9381505"/>
    <dbReference type="PaxDb" id="273116-14324932"/>
    <dbReference type="DNASU" id="1441822"/>
    <dbReference type="GeneID" id="1441822"/>
    <dbReference type="KEGG" id="tvo:TVG0723097"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_16_2_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="PhylomeDB" id="Q97AU3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964:SF113">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="142" mass="15449" checksum="4ED3949CA12539BC" modified="2001-10-01" version="1">MRVIVAYDGSPGADKAVGFALGLKDSIDKYFILYVSRDITDLSGQLVYISDETVEQQERLADEIVNKAKSLMTRSGVDYEVMKVDSNGEDISKVIVKIAQEKGIDVIFTGTRKLRGLDKFFLGSISSGILALSSCPVIVVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AU8</accession>
    <name>Q97AU8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase [UCPA]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0720035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AU8"/>
    <dbReference type="STRING" id="273116.gene:9381500"/>
    <dbReference type="PaxDb" id="273116-14324927"/>
    <dbReference type="KEGG" id="tvo:TVG0720035"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="PhylomeDB" id="Q97AU8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05344">
        <property type="entry name" value="BKR_like_SDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF6">
        <property type="entry name" value="NADPH-DEPENDENT REDUCTASE BACG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="256" mass="28402" checksum="7A33E5DC145625B2" modified="2001-10-01" version="1">MLFMTSSVIVSAASAGIGKGIVAVLLGYGYNITAFSRDESKLRALKEEMKLKFGREINTVVADLSVRSDLENVVSNHREKYGSIDNLVVNYGDPKVSSFIEISDEDWDYAISMMLKSTIYLTREALRDMIRTKHGSIVYVTSMTVREPIEGFSISASLRSAVISLAKTLTHEVSQNGIRVNTIAQGYFNTDRFRDIMKREIDSLSGMKRLAGEIPLRRVGEPEEIGELVEFLLSSRSSYINGANIPIDGGITKFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AW4</accession>
    <name>Q97AW4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0702334 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0702334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916955.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AW4"/>
    <dbReference type="STRING" id="273116.gene:9381483"/>
    <dbReference type="PaxDb" id="273116-14324911"/>
    <dbReference type="GeneID" id="1441803"/>
    <dbReference type="KEGG" id="tvo:TVG0702334"/>
    <dbReference type="HOGENOM" id="CLU_1438184_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="188" mass="21768" checksum="6CCE94A06F95ED31" modified="2001-10-01" version="1">MNLKDLGNFRIERTEDYSETEDKSYTEMIRVKGSKPELPYFKIPSHLYKYYETELALYLKDHKNYWRPLGKLLGEEIDITDEEIVLVFPVKMFSKVAEIVPFVRKRGQGNLKDDQKIKRLSRLKNTDNYPSKIEQKEPKYDLTELSRSIAPISTTHAHSALHDRLNEGDQNSGRDTAVPSACDGTDDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AX0</accession>
    <name>Q97AX0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0696965 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0696965</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AX0"/>
    <dbReference type="STRING" id="273116.gene:9381477"/>
    <dbReference type="PaxDb" id="273116-14324905"/>
    <dbReference type="KEGG" id="tvo:TVG0696965"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2784331_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="68" mass="8210" checksum="26C64CBD85D32893" modified="2001-10-01" version="1">MKKNASYKIIKEETSNSKIIEFNNGLILRLVTLDIDNKKRELVRTIYYLDSYQLEYIEIEKYKLEYNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AY3</accession>
    <name>Q97AY3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0675523</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
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        <property type="entry name" value="TauE"/>
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        <location>
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            <end position="57"/>
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        <location>
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        <location>
            <begin position="136"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
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        <location>
            <begin position="203"/>
            <end position="222"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="250" mass="26723" checksum="51B1BE293F172348" modified="2001-10-01" version="1">MSLFFLIIFIITWILSALFATAGVGAANTLIPIYYSMGIPFSIAAAAGLLLNVFSLSSATVNNGKRDRVLWKLGIIFLAPAVIMAPMGALVGIHTPRKVLLWMFVMFLGYTLYNLIRGKAKGNSDKFSVNAKGYALGVLVGAIAGFLGGLLGVGGGMIILPVLALIEKDYKKVSATAGFIALFSSASGFTSYLFLLKGISYNLWLIILIGGILGGFTGSYLMNKMKTIYVKYTIILIIAFVLIKILVGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AZ9</accession>
    <name>Q97AZ9_THEVO</name>
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        <submittedName>
            <fullName evidence="9">Multidrug-efflux transporter</fullName>
        </submittedName>
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        <name evidence="9" type="ORF">TVG0653656</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF46">
        <property type="entry name" value="BLR6921 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
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            <end position="29"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAB59802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="473" mass="51216" checksum="38A0AEA02CAF6AF7" modified="2001-10-01" version="1">MDGAYKLLAISVTSIGSFLAVINSTSLLISLPTIMISLHTTFLFIIWVLVIYPLVMTIGSPIFGRYSDFIGRKQLYGLGYIFFTIGSLLASSSINIDMLIAARAIQGLGGSLLFSNSLAIIADAFRQDELKWAIGTNSLIIGFATAIGPLVGGILTVLNWRYVFVFNVPFGIAGYFMSLKIHDIEKRQVKAAKLPSVFFSIFIILLIIYMTFAPPNNWISFSGLAYIISIAVSFILFIYFTGRSENSLIDISLFKIREFAVQAISTFLNSITRYSILLIAIIMLQGPLSLSPLTSSLMVMPYAVALSLASYFVGRVHRKGMEDHLIYAGLFLSFFGSFMLFFVRDASLPFYIGITLVGFGIGIFYTPSNAILMSTAPPNKRGSAAGLRTVILNLGSVIGMSMVFSVVAALVPESALSSAFLGVKIAQVNAVKPDFLDGIGLSFLISGLSSLLAAVIVSISHMKRAKSSAVSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B10</accession>
    <name>Q97B10_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">TVG0645074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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    <dbReference type="AlphaFoldDB" id="Q97B10"/>
    <dbReference type="STRING" id="273116.gene:9381437"/>
    <dbReference type="PaxDb" id="273116-14324865"/>
    <dbReference type="GeneID" id="1441756"/>
    <dbReference type="KEGG" id="tvo:TVG0645074"/>
    <dbReference type="eggNOG" id="arCOG00755">
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    <dbReference type="HOGENOM" id="CLU_007884_4_1_2"/>
    <dbReference type="OrthoDB" id="168391at2157"/>
    <dbReference type="PhylomeDB" id="Q97B10"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF287">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="417" mass="46619" checksum="69C385A326DF9085" modified="2001-10-01" version="1">MRYDVAIIGAGIVGLSAAFHLSEENPDLKILVLDKFHTYAQGNTGKSAAGFRDVFSSDISYKLSSSSIKFYDHVQNALGIDLGMHYVGYLFLLDDEKNAGVLEKIGKKTSISEVDLDSLNDMGISIKVDEETKKVMGISDIKSAYIGKNCGIMEPDKIAAFYYKECLKRGVEFHFDTNVQKLNLEPKEPLNFPGEPFIWQDKYIKSVTTDKGDIEADTFVLATDVWTNFLTDRIGVDSHIRPKKRQLFKIKNEFIASVVSKSFIGTESFPFTVFPKGVYIRPAPSEKTFWAGVADDIGRDFSFSEDPEAEEQFYTYNVYQVLQAYMKQLSSSSISGMWAGYYSYNTIDGNPYIFRSLNMIIATGTSGSGIMKGDAIGRVVSSLYRGKDFTYLYGGHGIKTEDLGVHARHVDMEELVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B14</accession>
    <name>Q97B14_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0641800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B14"/>
    <dbReference type="STRING" id="273116.gene:9381433"/>
    <dbReference type="PaxDb" id="273116-14324861"/>
    <dbReference type="KEGG" id="tvo:TVG0641800"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_16_2_2"/>
    <dbReference type="PhylomeDB" id="Q97B14"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="150" mass="16556" checksum="E8C3BD1F88E84CB9" modified="2001-10-01" version="1">MMKALIAYDGSETADCALEYALNFRKSIDKYFVIYIIPQIIGTTPTYDTYVPESMFEDQRKKGEEIIERAKKLVSNESVDLEFEIVDSGTKTVAHKAVEVAEEKGVDIIITGSRNLKGLSKIILGSVSSEIIKLSSVPVLVCSTNACKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B17</accession>
    <name>Q97B17_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0635229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916901.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B17"/>
    <dbReference type="SMR" id="Q97B17"/>
    <dbReference type="STRING" id="273116.gene:9381430"/>
    <dbReference type="PaxDb" id="273116-14324858"/>
    <dbReference type="GeneID" id="1441749"/>
    <dbReference type="KEGG" id="tvo:TVG0635229"/>
    <dbReference type="eggNOG" id="arCOG01669">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169424_0_0_2"/>
    <dbReference type="OrthoDB" id="216309at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="115" mass="12613" checksum="D5CB1DC6685E84F5" modified="2001-10-01" version="1">MAKMLVMVITGKENINTEMVAFNFAINSVQNAKATVEMLFLGRGVEAILKNQNNSDTFLAQINKARSLGIRLTVCSVSMKSLGIEDSMIFENIEKVMGGVETNAKIEEGYSVITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B23</accession>
    <name>Q97B23_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyl coenzyme A thioester hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0629193</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916895.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B23"/>
    <dbReference type="STRING" id="273116.gene:9381424"/>
    <dbReference type="PaxDb" id="273116-14324852"/>
    <dbReference type="GeneID" id="1441743"/>
    <dbReference type="KEGG" id="tvo:TVG0629193"/>
    <dbReference type="eggNOG" id="arCOG00773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050164_3_1_2"/>
    <dbReference type="OrthoDB" id="15030at2157"/>
    <dbReference type="PhylomeDB" id="Q97B23"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052816">
        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049:SF24">
        <property type="entry name" value="CYTOSOLIC ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="3">
        <location>
            <begin position="7"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="165" mass="19195" checksum="5392888A47B2768D" modified="2001-10-01" version="1">METKKPSESETFVERMVLPEDMDIYEYLYGGRLMEWIDNCASIVATKHCRKRTVTGSIDSLFFLLPIHLGDMVLLHGYINYTTKSTMEIEIDVIKQEGLTGIRRYATKAYLTYVAIDADGRPTEIPQIEPETDDEKRRYAEAEKRAEERRKRLETIKQQILNSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B27</accession>
    <name>Q97B27_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uroporphyrinogen-III synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0625033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916891.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B27"/>
    <dbReference type="STRING" id="273116.gene:9381420"/>
    <dbReference type="PaxDb" id="273116-14324848"/>
    <dbReference type="GeneID" id="1441739"/>
    <dbReference type="KEGG" id="tvo:TVG0625033"/>
    <dbReference type="eggNOG" id="arCOG02048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243061_0_0_2"/>
    <dbReference type="OrthoDB" id="57038at2157"/>
    <dbReference type="PhylomeDB" id="Q97B27"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004591">
        <property type="entry name" value="PRK05928.3-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40082">
        <property type="entry name" value="BLR5956 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40082:SF1">
        <property type="entry name" value="BLR5956 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="232" mass="26388" checksum="89625AE40A97DEA8" modified="2001-10-01" version="1">MTLRKFIISLRPAEKFVKFDSPCFDLINIPVTRLMPLDINIEKILKESAADCIVFTSTFGVDLFFSRVNMKGFTILGIGKATCERVQAYGHKCLYPENMDSEGLANLIISKCKDKKILILRSKKANEVLTEKLKEEGIDFLEFRIYDAVPDTKDFEKHYKDQNCLGVIITSSMEAEIVIPFLKEDKKMVFSIGVTTTNTLMSHGIMPDITGNSDFLELLKKIDYFICNAENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B31</accession>
    <name>Q97B31_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0621501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916887.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B31"/>
    <dbReference type="STRING" id="273116.gene:9381416"/>
    <dbReference type="PaxDb" id="273116-14324844"/>
    <dbReference type="GeneID" id="1441735"/>
    <dbReference type="KEGG" id="tvo:TVG0621501"/>
    <dbReference type="eggNOG" id="arCOG04734">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071501_1_0_2"/>
    <dbReference type="OrthoDB" id="56895at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="65"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="233" mass="26591" checksum="BCA1343591D00E4E" modified="2001-10-01" version="1">MQTEDNEDAFGHALLDFSSGHCSYEMIETSDGKIDVTSISYYFKNYDQWPLMEQKAIEYAKGIIVDAGAGAGRHSLYLRKKGYQVFPFDISPGAVSVMKKRGLDNASIMSLEEFDMSFDTLLLLGNNFGLLRSYKKAPIYLRKLYEKSSDHAMIIGETTDPVSTRFASNDSGLEAGDLTIRVVYKEYKTPWFEYMLASKEKLEMLVKDTGWRVKKFIEMDGVDVYCFLLEKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B35</accession>
    <name>Q97B35_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="4">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">TVG0617128</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916883.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B35"/>
    <dbReference type="STRING" id="273116.gene:9381412"/>
    <dbReference type="PaxDb" id="273116-14324840"/>
    <dbReference type="GeneID" id="1441731"/>
    <dbReference type="KEGG" id="tvo:TVG0617128"/>
    <dbReference type="eggNOG" id="arCOG04415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027420_3_0_2"/>
    <dbReference type="OrthoDB" id="146558at2157"/>
    <dbReference type="PhylomeDB" id="Q97B35"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="110"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB59766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="435" mass="48101" checksum="D69E6DF71F7AA241" modified="2001-10-01" version="1">MVSRVLLIGSGGREDAIARAIKRSGAKLFSVIGHENPSIKRLSEKYLIGDEKDYKSIEDFALSSSVDIVFVGPDPVLETPLVNNLLKRGIAVASPTMEAAQIETSKMFMRDLLTRHSIPGNINYKKCFSEQEVKDFFDKNKKDVAVKPIGLTGGKGVKVMGEQLKSQGEAINYAIEVLQRDGVVLIEDRMIGEEFSLQAFTDGKNTSFMPIVQDYKRAFEGDTGPNTGGMGSISDVNFSLPFLSKKAPEDARHIINDVVRAMYDENIPFKGVMYGQFMDTPDGVKVIEINARFADPEGINVLSLLKSDFVETLYQIYSGELNGERLFEDRATVLKYIVPPGYGQAPLPGDLHIDPEIEKLDTELYYAAVSGTLYDVKMSSSRSLAILGKADNIPEASEMVDSALKYVHGSYYVRRDIGKKEFLEKKIMAASRAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B70</accession>
    <name>Q97B70_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0575781 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0575781</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B70"/>
    <dbReference type="PaxDb" id="273116-14324802"/>
    <dbReference type="KEGG" id="tvo:TVG0575781"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393644_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97B70"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="195" mass="22985" checksum="4B7068800B8B0DB5" modified="2001-10-01" version="1">MIPQVSRSQSEVTIWETNGTERERNGISYMRLYPSVMLFNITDDHVHDVMVGRKIPESIKDRIAKIFRERRYDSKAIFNDFRSRKVIHPGRNASSRSGCSQARASIVRLIKKTYEEEWKESVQYGKRWNVVIYFLGLKRTMGEVIKAVRSEYIVQEIALKVQYYKIMRDNPCVLNCATYLFVTYADTRNNIERVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B72</accession>
    <name>PYRH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uridylate kinase</fullName>
            <shortName evidence="1">UK</shortName>
            <ecNumber evidence="1">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Uridine monophosphate kinase</fullName>
            <shortName evidence="1">UMP kinase</shortName>
            <shortName evidence="1">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrH</name>
        <name type="ordered locus">TV0585</name>
        <name type="ORF">TVG0573246</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UMP kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916844.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B72"/>
    <dbReference type="SMR" id="Q97B72"/>
    <dbReference type="STRING" id="273116.gene:9381372"/>
    <dbReference type="PaxDb" id="273116-14324800"/>
    <dbReference type="GeneID" id="1441692"/>
    <dbReference type="KEGG" id="tvo:TVG0573246"/>
    <dbReference type="eggNOG" id="arCOG00858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079546_0_0_2"/>
    <dbReference type="OrthoDB" id="372251at2157"/>
    <dbReference type="PhylomeDB" id="Q97B72"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006225">
        <property type="term" value="P:UDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04253">
        <property type="entry name" value="AAK_UMPK-PyrH-Pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_A">
        <property type="entry name" value="PyrH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011818">
        <property type="entry name" value="Uridylate_kinase_arch/spir"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02076">
        <property type="entry name" value="pyrH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833">
        <property type="entry name" value="URIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833:SF4">
        <property type="entry name" value="URIDYLATE KINASE PUMPKIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000143930" description="Uridylate kinase">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="114"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <sequence length="227" mass="24333" checksum="6F4FE4895CEE6E81" modified="2001-10-01" version="1">MNESIVISLGGSVISGDPIDADYLQSFAKILASSKFKRIGIVTGGGKTARSYISLLRSLGINENMLDEIGIYATRMNALSLASLLKGANPIIPSTVEEAVNLMSEYRFVVMGGTEPGHTTDTVAALLCERSDTDTLINITSVDGVYDLDPNKYKDARRFDTLGYREAITLSTGSSVGAGPNVFMDITALSIAMRSKIKVIVASRDLNNLKNILEGKPSVFTSIEEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BE4</accession>
    <name>Q97BE4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transport system ATP-binding protein A1A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0501242</name>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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            <taxon>Methanobacteriati</taxon>
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            <taxon>Thermoplasmata</taxon>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BE4"/>
    <dbReference type="STRING" id="273116.gene:9381295"/>
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    <dbReference type="KEGG" id="tvo:TVG0501242"/>
    <dbReference type="eggNOG" id="arCOG07874">
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    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="OrthoDB" id="57213at2157"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
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    </dbReference>
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        <property type="entry name" value="AAA"/>
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    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="257" mass="29248" checksum="C014206011B50495" modified="2001-10-01" version="1">MRLYIENISKVYKRRTSAFKWVDIPALKNISYEFDEGTIYGVIGPSGSGKTTLANISVLYDRPDSGSVYYGNKEVTRMKPADLKSYAGIVRYVFQDPYTSLEPLRTVGWHIDTAAQSIHIVQEEAWKIATYLGMPESRYRDSIVEDLSGGQRQRLAFIIAMIADPAIIILDEPFSMLDSYNSMFLLNYLRMNKKGRVIIYIDQNIGKVLYVADRILVMDNGSIVEDQELSGILSTPSHETTKKLLKSYRKIYDGLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BI0</accession>
    <name>Q97BI0_THEVO</name>
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            <taxon>Archaea</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59617.1"/>
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    </dbReference>
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    <dbReference type="PaxDb" id="273116-14324690"/>
    <dbReference type="GeneID" id="24779896"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="95" mass="10845" checksum="4FA38B9E5979EABB" modified="2001-10-01" version="1">MSSEINEEKLNTTMEERRKKEPERKNEGGIKAFILSMVAIVVITAALTSGTYKGYDPNPYSVAITGFFAIVTIVMTAVWYRSKYNNYFYNSDQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BI6</accession>
    <name>Q97BI6_THEVO</name>
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    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="DNASU" id="1440987"/>
    <dbReference type="GeneID" id="1440987"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="208" mass="24027" checksum="174FF032BEBDD9CD" modified="2001-10-01" version="1">MLENYGIAILEREGEYIIKPFFSGIPKISTLSIIYDRNLEYEQARKEILDFLKLSGIKTEFVGIGDVNNFFQVFLILQKICKTKGIPRWINVSCGSGLGVSALTIHAFKQNIPMVIYDKYIDKTVMIEAKRLKKINIYNNKYITLIREISKDPKTIKDLSRSLKIDTSTISRRLKSLLSLDMIMRLGKGTRNYPYLYKLNDFGKILLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BM7</accession>
    <name>Q97BM7_THEVO</name>
    <protein>
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        <name evidence="2" type="ORF">TVG0415361</name>
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    <organism evidence="2 3">
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        <lineage>
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            <taxon>Thermoplasmata</taxon>
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            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BM7"/>
    <dbReference type="STRING" id="273116.gene:9381207"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324643"/>
    <dbReference type="KEGG" id="tvo:TVG0415361"/>
    <dbReference type="eggNOG" id="arCOG03285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028187_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BM7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF13">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="1">
        <location>
            <begin position="307"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="659" mass="76598" checksum="36101F5405F13B33" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BP1</accession>
    <name>RS27_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps27e</name>
        <name type="ordered locus">TV0414</name>
        <name type="ORF">TVG0402231</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916671.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BP1"/>
    <dbReference type="SMR" id="Q97BP1"/>
    <dbReference type="STRING" id="273116.gene:9381192"/>
    <dbReference type="PaxDb" id="273116-14324629"/>
    <dbReference type="GeneID" id="1440929"/>
    <dbReference type="KEGG" id="tvo:TVG0402231"/>
    <dbReference type="eggNOG" id="arCOG04108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199465_0_0_2"/>
    <dbReference type="OrthoDB" id="5718at2157"/>
    <dbReference type="PhylomeDB" id="Q97BP1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001629">
        <property type="entry name" value="PRK00415.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000149085" description="Small ribosomal subunit protein eS27">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00371"/>
        </source>
    </evidence>
    <sequence length="65" mass="6853" checksum="C69FB56E3E76991E" modified="2001-10-01" version="1">MSEVKFVKPKNVSGHFLKIKCKDCGNVQVVFARPSSVVTCNICGATIAKPTGGVLETSGEVIESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BP5</accession>
    <name>Q97BP5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Anhydromevalonate phosphate decarboxylase</fullName>
            <ecNumber evidence="10">4.1.1.126</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">TVG0397730</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of trans-anhydromevalonate 5-phosphate (tAHMP) into isopentenyl phosphate. Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2E)-3-methyl-5-phosphooxypent-2-enoate + H(+) = isopentenyl phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:78971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:229665"/>
            <dbReference type="EC" id="4.1.1.126"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:78972"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>prenylated FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.126" evidence="10"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916666.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BP5"/>
    <dbReference type="STRING" id="273116.gene:9381188"/>
    <dbReference type="PaxDb" id="273116-14324625"/>
    <dbReference type="DNASU" id="1440924"/>
    <dbReference type="GeneID" id="1440924"/>
    <dbReference type="KEGG" id="tvo:TVG0397730"/>
    <dbReference type="eggNOG" id="arCOG01671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023348_4_1_2"/>
    <dbReference type="OrthoDB" id="8480at2157"/>
    <dbReference type="PhylomeDB" id="Q97BP5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1670.10:FF:000003">
        <property type="entry name" value="Phenolic acid decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1670.10">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022390">
        <property type="entry name" value="HBDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049381">
        <property type="entry name" value="UbiD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049383">
        <property type="entry name" value="UbiD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048304">
        <property type="entry name" value="UbiD_Rift_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03701">
        <property type="entry name" value="mena_SCO4490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00148">
        <property type="entry name" value="UbiD family decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="3-OCTAPRENYL-4-HYDROXYBENZOATE CARBOXY-LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108:SF17">
        <property type="entry name" value="FERULIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20696">
        <property type="entry name" value="UbiD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20695">
        <property type="entry name" value="UbiD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143968">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like N-terminal" evidence="14">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like Rift-related" evidence="13">
        <location>
            <begin position="128"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like C-terminal" evidence="15">
        <location>
            <begin position="320"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAB59552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="481" mass="54717" checksum="755CE873A9FDE140" modified="2001-10-01" version="1">MTFEDLHEYLDYLAKKNDLVTITEEVDPNLDLTYILSEEERMGRGRTILFKNVKGSQVPAVGNLFSTNEKLKAVLGDDPYSIGVKIADIVKPPRDSESFIGKGLEMMRELSGLRPKVSNSIPSGYSELEKVDLYRYPICKTWPDDGGPFITLPLVITKDPSTGIRNMGMYRMQVYDSETTGMHWHIHKGGSENFLKEKEKGKAMDVAVVIGSDPLTIFSAVAPLPNGIDEFMFRGLISRKRSELVKGKTVDLEYPRNFEIVLEGYIDPSETRVEGPFGDHTGYYSLEEEFPVFHVKNIIERNDRIYPTTIVGKLWHEDVVLGKAVERMFLPLIQMVLPEVVDINTMEEAVFHNMVIVSIKKRYPGHAKKVMFALWGLGQLMFSKIIVVVDDDINIHNRKELIWAMTTRIDPDRDVIIIPGTVTDSLDHAAPLFNYGSKMGIDATKKDKSEGYTRRWPDVLEMPKDVVDKVRQIMKNNRLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BQ2</accession>
    <name>Q97BQ2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RAS-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0391697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916659.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BQ2"/>
    <dbReference type="STRING" id="273116.gene:9381181"/>
    <dbReference type="PaxDb" id="273116-14324618"/>
    <dbReference type="GeneID" id="1440917"/>
    <dbReference type="KEGG" id="tvo:TVG0391697"/>
    <dbReference type="eggNOG" id="arCOG05343">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559572_0_0_2"/>
    <dbReference type="OrthoDB" id="56314at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="171" mass="19132" checksum="EFB162C82FD674BC" modified="2001-10-01" version="1">MPIQRLAWKFVIAGSKGAGKSSFISYIVYGEPAPILPRSLVKKSLNREINGHKLAVDILFQEVDGGIENFIPTATAFIIVVDVTSADSLSEARNIIMEIKNAKKNNIFVIGNKIDLKYEAQIWIDDMEELKNKFSTKYYMVSCKDGIGFETLLEDIINSIATKTQARRANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BQ8</accession>
    <name>Q97BQ8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0385916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BQ8"/>
    <dbReference type="STRING" id="273116.gene:9381175"/>
    <dbReference type="PaxDb" id="273116-14324612"/>
    <dbReference type="KEGG" id="tvo:TVG0385916"/>
    <dbReference type="eggNOG" id="arCOG01842">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128031_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BQ8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="20"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="139" mass="16219" checksum="50FE667073B4A553" modified="2001-10-01" version="1">MVCYIMAKFPSDEWVSEYCNRLNNNPDYADAGKTWEGDILFVVQPDENYSKTEYIYIDLYHGKCRSAKYVHEGEEVPKAEFRYVGKYGNWIRLLNKEIDPIQGILTGKFKLEGSMMKIMRYTKAAKEMVNTAAQVKTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BR9</accession>
    <name>Q97BR9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">26S proteasome regulatory subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0373521</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916640.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BR9"/>
    <dbReference type="STRING" id="273116.gene:9381163"/>
    <dbReference type="PaxDb" id="273116-14324601"/>
    <dbReference type="GeneID" id="1440898"/>
    <dbReference type="KEGG" id="tvo:TVG0373521"/>
    <dbReference type="eggNOG" id="arCOG01307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_21_15_2"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="PhylomeDB" id="Q97BR9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001054">
        <property type="entry name" value="ATPase, AAA family, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="144"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="375" mass="42005" checksum="2315B804383B4180" modified="2001-10-01" version="1">MSDDVQNKIEKATKLLHAGIKEEENGHKDKAKEYYLVAYRVMLEAANDSPSDLKKKRLDQCALILNAYKRVSGERTDFTVKKQNDDEIVEGEALLEEIGIEKPEIPKVTFEDVAGLDDVKNEILGKIIYPMKFKELSQEYNIQFGGGMLLYGPPGTGKTFIVKAIANEVKARFINVNPSTLYSEWFGVFEKNISKLFRAASLLSPAIIFFDEIDALVPKRDTSNSDAAKRGVAQLLNEVGGINSQKNKNIFIIAATNNPWEVDEAMLRPGRFDIKIYVPPPDIVARKKIFQLNLAKIKQVGNVDYDLLAEETEGYSGADIEFICKKAAQNVFMEAVKTGKSRPVETRDVIDVIGSIKPSIDYELLEKYSKYGSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BT5</accession>
    <name>Q97BT5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Rieske iron sulfur protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0360023</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_156769046.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BT5"/>
    <dbReference type="STRING" id="273116.gene:9381147"/>
    <dbReference type="PaxDb" id="273116-14324585"/>
    <dbReference type="GeneID" id="1440882"/>
    <dbReference type="KEGG" id="tvo:TVG0360023"/>
    <dbReference type="eggNOG" id="arCOG01720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074282_0_0_2"/>
    <dbReference type="OrthoDB" id="5623at2157"/>
    <dbReference type="PhylomeDB" id="Q97BT5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03475">
        <property type="entry name" value="Rieske_SoxF_SoxL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="7">
        <location>
            <begin position="146"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="265" mass="27971" checksum="2676DAEEC523C9CF" modified="2001-10-01" version="1">MRRCHDTTEEKKNAKCKNQSENDVVNDPSRRNFIKAMGIISIAAVAGLSLRGLIQNIIPPQEVVSTGFPTLTLVSQKTGAPVKTSDLQVNSPEIVLFDYPLQNEPNFLLRLGNSSGQDIQIKPVSVKIPATGKTYTSPGGVGPYKSVVASSAICQHLGCVPPEIHYYPPGTPIPSTSISGSSNHGLIHCNCHGSTYDPLNGFSVVTGPTTHPLPSVVLSYDSSTDTYKAVSMVGPTIYGHTNDLSGGNPVSGTSTDVVNEGVPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BX8</accession>
    <name>Q97BX8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cas12f1-like TNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0331810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916581.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BX8"/>
    <dbReference type="PaxDb" id="273116-14324542"/>
    <dbReference type="GeneID" id="1440839"/>
    <dbReference type="KEGG" id="tvo:TVG0331810"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881184_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BX8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="30"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="135" mass="15134" checksum="1C5F681C1C33B7FA" modified="2001-10-01" version="1">MRIGGINKITKKGDGKGNNYRFILKNAFPYGMLASQVMYKASWEGLPAIELTRKETRNTSRMCSVCDTLARIEHGRILKCDSCGIEIDRDVNACINIAFRYRTCLKRSHKGLPGEAVKQSKDVEQMAGSQISMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BY9</accession>
    <name>Q97BY9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0323462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916572.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BY9"/>
    <dbReference type="STRING" id="273116.gene:9381091"/>
    <dbReference type="PaxDb" id="273116-14324531"/>
    <dbReference type="GeneID" id="1440830"/>
    <dbReference type="KEGG" id="tvo:TVG0323462"/>
    <dbReference type="eggNOG" id="arCOG04130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076814_1_0_2"/>
    <dbReference type="OrthoDB" id="7902at2157"/>
    <dbReference type="PhylomeDB" id="Q97BY9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007003">
        <property type="entry name" value="DUF655"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04919">
        <property type="entry name" value="DUF655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160975">
        <property type="entry name" value="AF1531-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="182" mass="21516" checksum="C34CDFCCA570432F" modified="2001-10-01" version="1">MEEYAYVLDYLPQGRPDDRSYRMNPLVLAIGESEFKLLELIPKPNEIITVGEKVYIGKNPEMRKKILSVRRRITYKDLTNSAQNELPYVLEDIVNKQQERFIKFFNEAEPINARLHSLELLPGLGNKTMWAILEERKKKPFDSFEDLVNRVKSIHNPQKMIAGRIIEELKDRYEKYKLFVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BZ0</accession>
    <name>Q97BZ0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo4</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo4</name>
        <name evidence="1" type="synonym">rpoF</name>
        <name evidence="2" type="ORF">TVG0322900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit is less well bound than the others.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex. Forms a stalk with Rpo7 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916571.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BZ0"/>
    <dbReference type="STRING" id="273116.gene:9381090"/>
    <dbReference type="PaxDb" id="273116-14324530"/>
    <dbReference type="GeneID" id="1440829"/>
    <dbReference type="KEGG" id="tvo:TVG0322900"/>
    <dbReference type="eggNOG" id="arCOG01016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178229_0_0_2"/>
    <dbReference type="OrthoDB" id="56017at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00864">
        <property type="entry name" value="RNApol_arch_Rpo4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010924">
        <property type="entry name" value="Rpo4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011557">
        <property type="entry name" value="PRK14981.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT RPO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646">
        <property type="entry name" value="RNA POLYMERASE RPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005053">
        <property type="entry name" value="RNA_pol_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="103" mass="11636" checksum="CD7ABDCD29F47DA1" modified="2001-10-01" version="1">MEKKYISIPEVYNILSKKEQLTDMEKENLEYAGKFSRVDPSVIDRVKGDISGIAKLPEKVIVKILDLKPGTPADITSILSTYNVMISDEDLNALADYLKKLKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-10" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BZ8</accession>
    <name>PSA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">20S proteasome alpha subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome core protein PsmA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psmA</name>
        <name type="ordered locus">TV0307</name>
        <name type="ORF">TVG0317752</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916562.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BZ8"/>
    <dbReference type="SMR" id="Q97BZ8"/>
    <dbReference type="STRING" id="273116.gene:9381082"/>
    <dbReference type="MEROPS" id="T01.970"/>
    <dbReference type="PaxDb" id="273116-14324522"/>
    <dbReference type="GeneID" id="1440820"/>
    <dbReference type="KEGG" id="tvo:TVG0317752"/>
    <dbReference type="eggNOG" id="arCOG00971">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_2"/>
    <dbReference type="OrthoDB" id="9421at2157"/>
    <dbReference type="PhylomeDB" id="Q97BZ8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03756">
        <property type="entry name" value="proteasome_alpha_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00289_A">
        <property type="entry name" value="Proteasome_A_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019982">
        <property type="entry name" value="Proteasome_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03633">
        <property type="entry name" value="arc_protsome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000124191" description="Proteasome subunit alpha">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00289"/>
        </source>
    </evidence>
    <sequence length="233" mass="25544" checksum="7CC3948B8BDEAA01" modified="2002-05-10" version="2">MQQGQMAYDRAITVFSPDGRLFQVEYAREAVKKGSTALGLKFSNGVLLISDKKIRSKLIEQNSLEKIQLIDDYVAAVTSGLVADARVLIDFARISAQQEKVTYGSLVNIENLVKRVADQMQQYTQYGGVRPYGVSLIFAGIDQIGPRLFDCDPAGTINEYKATAIGSGKDAVVSFLEKEYKEGLSEKEAVTLGIKALKASLEEGEELKAPEIASITVGSKYRIYGAEEVKKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C02</accession>
    <name>Q97C02_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0313402 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0313402</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C02"/>
    <dbReference type="PaxDb" id="273116-14324518"/>
    <dbReference type="KEGG" id="tvo:TVG0313402"/>
    <dbReference type="eggNOG" id="arCOG07410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623537_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="191" mass="22586" checksum="56FFC0EC3CA9639B" modified="2001-10-01" version="1">MLIIQRYIKHKVRLIGKFINDNIMILAIMTQENKTISKRSEDEVLEGLLRRIAQLKRNRELNYRKMANSNVPDNVRSMMLRVADMEEQTRRRIEDAIRKKEFDVSINNTNSYAMIEHIVKNSDVSDISDVKSVLLKSMKEEEELHRILEVMSKEYNGTNIEKTLRSIIECEENNKNALADLYEDYVNEGYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C06</accession>
    <name>Q97C06_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="8">CDG synthase</shortName>
            <ecNumber evidence="8">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Archaeosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">queE</name>
        <name evidence="10" type="ORF">TVG0308581</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-carbaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="8">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="8"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916554.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C06"/>
    <dbReference type="STRING" id="273116.gene:9381073"/>
    <dbReference type="PaxDb" id="273116-14324514"/>
    <dbReference type="DNASU" id="1440812"/>
    <dbReference type="GeneID" id="1440812"/>
    <dbReference type="KEGG" id="tvo:TVG0308581"/>
    <dbReference type="eggNOG" id="arCOG02173">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066739_2_3_2"/>
    <dbReference type="OrthoDB" id="7980at2157"/>
    <dbReference type="PhylomeDB" id="Q97C06"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836:SF1">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000370">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="16"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="209" mass="23893" checksum="B38BC97CAF93AB0E" modified="2001-10-01" version="1">MLITEIFHSIQGEGTLIGIPMLFVRTNVCNIRCEWCDTKYSFYGGREIALSDILNIVKEAKEQWVCFTGGEPLVQRDALSFVEGSLKLGKKILIETNGTVPIRNFTISDNIVLDVDVKSPSAKVRKPFLEENLKYIRDTDYLKIVIKDREDLDFAINFIRGHRKNLNYVLQPAWGSDIRMIADAIVETDLNVRVLPQLHKIMYGEMRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C54</accession>
    <name>Q97C54_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mercuric reductase</fullName>
            <ecNumber evidence="4">1.16.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Hg(II) reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">TVG0264489</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="20 21" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Hg + NADP(+) + H(+) = Hg(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23856"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16170"/>
            <dbReference type="ChEBI" id="CHEBI:16793"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.1" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916509.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C54"/>
    <dbReference type="STRING" id="273116.gene:9381023"/>
    <dbReference type="PaxDb" id="273116-14324465"/>
    <dbReference type="GeneID" id="1440767"/>
    <dbReference type="KEGG" id="tvo:TVG0264489"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_1_1_2"/>
    <dbReference type="PhylomeDB" id="Q97C54"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016152">
        <property type="term" value="F:mercury (II) reductase (NADP+) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045340">
        <property type="term" value="F:mercury ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050787">
        <property type="term" value="P:detoxification of mercury ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
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        <property type="entry name" value="Mercury_reductase_MerA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02053">
        <property type="entry name" value="MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43014">
        <property type="entry name" value="MERCURIC REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43014:SF4">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE RCLA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="7 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="11" id="KW-0476">Mercury</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="12 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14 17" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="19">
        <location>
            <begin position="6"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="18">
        <location>
            <begin position="347"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BAB59393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="471" mass="51011" checksum="514B7DA73730D674" modified="2001-10-01" version="1">MLSEKYDLVIVGRGAAAFSAAIRASEITSGQARIAMVGNGPLGGTCVNTGCVPSKLLISISNSFQNSLKPRYPGLPSVERPPETEVIMDFIRESVHGERKKKYEDVVESYENIDLYTGTAVFTGEKEILVDSSHSLFGYNVLIATGSRPYVPGIKGLNETSYITTDSVWELKHVPASIAILGGGAVGVEIGQALSRLGSEVHIIEVSNQILPGIPADMASIIERSLREDGMVINTSTSVDEVSGGEGKKYLKLNGTNINDTLEVEEILVATGRAPNIDLALEKTGVKYSKRGIAVDEYLRTSNRKIYAAGDVVDQKYKLETLAAREGFISAENMFNHAMRTIDIFNVPFAVFCSPGYASVGYTQDEATKAGIKTEARRISTKDVASERIHANREGIINIVAEADTKEIVGVQIVAENAPELINEASAILSKRFKTDDLINTVHVFPTENEGLKLATQSFSRDITMMSCCME</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C59</accession>
    <name>Q97C59_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0260158</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C59"/>
    <dbReference type="STRING" id="273116.gene:9381017"/>
    <dbReference type="PaxDb" id="273116-14324460"/>
    <dbReference type="KEGG" id="tvo:TVG0260158"/>
    <dbReference type="eggNOG" id="arCOG02686">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="PhylomeDB" id="Q97C59"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="5"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="416" mass="46438" checksum="48D1B98ABBBEF80E" modified="2001-10-01" version="1">MGYSIAIIVSASIFLDVWDLASLSFVITFFRSYFYLSGLTLSVAVSSANIGAVMGSISGAYLVERFGRKRMLLSNMLIFFASSIAIVFSPSIYLVIPFRILMGFGIGSDVVTGFTYIYEYANERQRQTFYPLWGYSFSAIAILAIVVVFVSHLFVTWDQLWKIVFMIAAIFSVSMLFLRYRLMETPLWLYLNRKYSELENVMEKAYGKTILSLSDPPSRERIFGDSLKFLRSKRMAFVLSLNGIVGFIGWGFSFYVTYMLSALHLYTFNSILGIDMIIYGSALIAAYISGYISKRFGVYKSTVVPSALSVASLLALVMVFLGYLPQLFVVPLIIAVIFFDYLGPMAYNAVLNIYVNPETRSAANGMNYMVNKVIESASGLSAGMILIEFGLGINTLMLTIIAGVLFVFAAYFGRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C71</accession>
    <name>Q97C71_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NADH oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0244368</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916488.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C71"/>
    <dbReference type="STRING" id="273116.gene:9381004"/>
    <dbReference type="PaxDb" id="273116-14324447"/>
    <dbReference type="GeneID" id="1440748"/>
    <dbReference type="KEGG" id="tvo:TVG0244368"/>
    <dbReference type="eggNOG" id="arCOG00615">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_1_2_2"/>
    <dbReference type="OrthoDB" id="24876at2157"/>
    <dbReference type="PhylomeDB" id="Q97C71"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OxRdtase_FMN_N"/>
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        <property type="entry name" value="2,4-DIENOYL-COA REDUCTASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42917:SF2">
        <property type="entry name" value="2,4-DIENOYL-COA REDUCTASE [(2E)-ENOYL-COA-PRODUCING]"/>
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    </dbReference>
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        <property type="entry name" value="FAD_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="314"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="547" mass="61141" checksum="AD8D498F86FE7261" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C95</accession>
    <name>Q97C95_THEVO</name>
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        <submittedName>
            <fullName evidence="2">TVG0213580 protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">TVG0213580</name>
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    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C95"/>
    <dbReference type="PaxDb" id="273116-14324422"/>
    <dbReference type="KEGG" id="tvo:TVG0213580"/>
    <dbReference type="HOGENOM" id="CLU_1691632_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="155" mass="17737" checksum="620C91A6C9FBB3CE" modified="2001-10-01" version="1">MLNYKKLVALFVIVTFSVIIVMSGYVYYHWGKSTDQPEEDILSNVYIGQVNINQSYTKIIFPHHINSGSDFNITILFNSNISINYVYTQTSGFCVLGWIFYFNTYIKYGTSQGKFIGSLEENIGILNITIKSPNYYYNGYISLHLVGSVPETVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C98</accession>
    <name>Q97C98_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transport system permease protein P1P2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0211687</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C98"/>
    <dbReference type="STRING" id="273116.gene:9380975"/>
    <dbReference type="PaxDb" id="273116-14324419"/>
    <dbReference type="KEGG" id="tvo:TVG0211687"/>
    <dbReference type="eggNOG" id="arCOG00157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_3_2"/>
    <dbReference type="OrthoDB" id="45815at2157"/>
    <dbReference type="PhylomeDB" id="Q97C98"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43005">
        <property type="entry name" value="BLR7065 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43005:SF1">
        <property type="entry name" value="SPERMIDINE_PUTRESCINE TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="243"/>
        </location>
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            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="76"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="296" mass="33309" checksum="792A50D9D6810096" modified="2001-10-01" version="1">MQRISGRNSILKKNAIFLILIPSFIYLIIFFIYPIFLSFKYSFTNLSLYDINKYSFIGFTNYVDLFKSDLFLKSIYITIIFLVVSAIIGQMFLGSILAYLLTLTHKYFKTVVTTIILIAWATPQVTAGIMWYSTLSYIPEGTINVILKNMGFHPIDFFSIHNAIYSIIIANIWIGLGFSVLIFLGGIQSIDPSIIKASYIDGAKPFRRFFSIIFPLLKTNIITDLLLITLFTLGTFTLIYVLTAGGPAGSTELLTIYQYETGFSLFSLGLANAIGVIIIIIALVLSLIYLRFIKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C99</accession>
    <name>Q97C99_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0210789 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0210789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916461.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C99"/>
    <dbReference type="STRING" id="273116.gene:9380974"/>
    <dbReference type="PaxDb" id="273116-14324418"/>
    <dbReference type="GeneID" id="1440721"/>
    <dbReference type="KEGG" id="tvo:TVG0210789"/>
    <dbReference type="eggNOG" id="arCOG00151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_11_0_2"/>
    <dbReference type="OrthoDB" id="56983at2157"/>
    <dbReference type="PhylomeDB" id="Q97C99"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050490">
        <property type="entry name" value="Bact_solute-bd_prot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649">
        <property type="entry name" value="ARABINOSE-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649:SF14">
        <property type="entry name" value="BLR3389 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="519" mass="56729" checksum="1A5271D3BB2E8EEF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-15" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CC2</accession>
    <name>IDI2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isopentenyl-diphosphate delta-isomerase</fullName>
            <shortName evidence="1">IPP isomerase</shortName>
            <ecNumber evidence="1">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl diphosphate:dimethylallyl diphosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Type 2 isopentenyl diphosphate isomerase</fullName>
            <shortName evidence="1">IDI-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fni</name>
        <name type="ordered locus">TV0180</name>
        <name type="ORF">TVG0186390</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer. Dimer of tetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP isomerase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916435.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CC2"/>
    <dbReference type="SMR" id="Q97CC2"/>
    <dbReference type="STRING" id="273116.gene:9380950"/>
    <dbReference type="PaxDb" id="273116-14324394"/>
    <dbReference type="GeneID" id="1441665"/>
    <dbReference type="KEGG" id="tvo:TVG0186390"/>
    <dbReference type="eggNOG" id="arCOG00613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065515_1_0_2"/>
    <dbReference type="OrthoDB" id="371955at2157"/>
    <dbReference type="PhylomeDB" id="Q97CC2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02811">
        <property type="entry name" value="IDI-2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00354">
        <property type="entry name" value="Idi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011179">
        <property type="entry name" value="IPdP_isomerase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02151">
        <property type="entry name" value="IPP_isom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43665">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43665:SF1">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003314">
        <property type="entry name" value="IPP_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000134458" description="Isopentenyl-diphosphate delta-isomerase">
        <location>
            <begin position="1"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="5"/>
            <end position="6"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="62"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="269"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="290"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00354"/>
        </source>
    </evidence>
    <sequence length="347" mass="38304" checksum="5806FEC1A3B779A0" modified="2001-10-01" version="1">MIEKRKEEHIRIAENENVSAFHNYWDDVYLMHEADPEVNYDDIDTGVDFLGKHLGFPMVISSMTGGAEIAKKINYNLATVAEKYQLAMGVGSMRAAIVNRSLSDTYSVINERNVPIKIANIGAPQLVPQGKEAIDEKDIAYIYDLIKADFLAVHFNFLQEMVQPEGDRNAEGVIKRIKELSGSFNIIAKETGSGFSKATAQRLADAGVKAIEVSGLSGTTFAAVEYYRAKNEGNAEKMRIGETFWNWGIPSPASVYYCSDVLPVIGSGGLRNGLDLAKAISLGASLGGFARTLLKDADQSVEAVSRNVEMIEREFKVAMFLTGNKNVYELRKTKKVIGEPLKEWMEV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CC6</accession>
    <name>DEOC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxyribose-phosphate aldolase</fullName>
            <shortName evidence="1">DERA</shortName>
            <ecNumber evidence="1">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphodeoxyriboaldolase</fullName>
            <shortName evidence="1">Deoxyriboaldolase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">deoC</name>
        <name type="ordered locus">TV0176</name>
        <name type="ORF">TVG0183359</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; 2-deoxy-D-ribose 1-phosphate degradation; D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-alpha-D-ribose 1-phosphate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeoC/FbaB aldolase family. DeoC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916431.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CC6"/>
    <dbReference type="SMR" id="Q97CC6"/>
    <dbReference type="STRING" id="273116.gene:9380946"/>
    <dbReference type="PaxDb" id="273116-14324390"/>
    <dbReference type="GeneID" id="1441661"/>
    <dbReference type="KEGG" id="tvo:TVG0183359"/>
    <dbReference type="eggNOG" id="arCOG04320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053595_0_2_2"/>
    <dbReference type="OrthoDB" id="31145at2157"/>
    <dbReference type="PhylomeDB" id="Q97CC6"/>
    <dbReference type="UniPathway" id="UPA00002">
        <property type="reaction ID" value="UER00468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006018">
        <property type="term" value="P:2-deoxyribose 1-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00959">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00114">
        <property type="entry name" value="DeoC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028581">
        <property type="entry name" value="DeoC_typeI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00126">
        <property type="entry name" value="deoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889:SF1">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001357">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000057293" description="Deoxyribose-phosphate aldolase">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with acetaldehyde" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00114"/>
        </source>
    </evidence>
    <sequence length="249" mass="27597" checksum="DD1A209F230431CD" modified="2001-10-01" version="1">MKYSLKTVMSLVDHSGLKPYLREDEIARLIGEASEMGNYSVCIEPIYGNFAVEYIKEKGYSLKVDVTLDFPFGSLPTSARKKIIEDSVYADEIDMVIPIGYVKSHRWDKVEQDINDVVSTARDLGLVSKIITEDGYLTLDEKLKTYDIVIRSAPDFIKTSTGFADKEFCKSLGNETGATPENVKLMSEIAKKIGSNIGIKAAGGIHTYDQVEKIIDSAGMIPEPSNIRLGMSGTKKLYEEMKKASVQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CC7</accession>
    <name>Q97CC7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cysteine-S-conjugate beta-lyase</fullName>
            <ecNumber evidence="2">4.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0183353</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.4.1.13" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CC7"/>
    <dbReference type="STRING" id="273116.gene:9380945"/>
    <dbReference type="PaxDb" id="273116-14324389"/>
    <dbReference type="KEGG" id="tvo:TVG0183353"/>
    <dbReference type="eggNOG" id="arCOG00060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CC7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000046">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005026">
        <property type="entry name" value="PRK06434.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF50">
        <property type="entry name" value="CYSTATHIONINE BETA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="394" mass="44164" checksum="688A57972A070AE3" modified="2001-10-01" version="1">MNKVRMAEKRLFNGFNTRAVQSGELRDPRFGNVTTPIFETSTFVFPNSEKDAYIDNTRNLPYIYTRWGNPTVQAFEEKYASLEKAEHALSFSSGMGAITSVILALAERGDKILSVSDLYGQTFYFLNKVLPEYGISVEYISTDRLNSGEFKPSDYDIIYLESITNPTLKVPDIKAVSERSTGKKIIVDATFASPYNQNPLDLGADVVIHSATKYISGHSDVVMGVFSTNDKSIFDKVVVRRKTFGSNPDPIQSYLALRGIKTLGLRMEKHNKNGMELAKYLRTEPRISHVYYPDTEIGKKVLRGFGGMISFELRPELDVHVFMRNLKIPMVAASLGGVESLITLPVETSHSSMTKEERERIGITDNLVRFSIGIEDHVDLIDDIKQALDKASES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CE5</accession>
    <name>DNAG_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase DnaG</fullName>
            <ecNumber evidence="1">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaG</name>
        <name type="ordered locus">TV0157</name>
        <name type="ORF">TVG0167197</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Also part of the exosome, which is a complex involved in RNA degradation. Acts as a poly(A)-binding protein that enhances the interaction between heteromeric, adenine-rich transcripts and the exosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.101"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds two Mg(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a ternary complex with MCM helicase and DNA. Component of the archaeal exosome complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CE5"/>
    <dbReference type="SMR" id="Q97CE5"/>
    <dbReference type="STRING" id="273116.gene:9380927"/>
    <dbReference type="PaxDb" id="273116-14324371"/>
    <dbReference type="KEGG" id="tvo:TVG0167197"/>
    <dbReference type="eggNOG" id="arCOG04281">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034626_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CE5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000010">
        <property type="entry name" value="DNA primase DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00007">
        <property type="entry name" value="DNA_primase_DnaG_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050219">
        <property type="entry name" value="DnaG_primase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020607">
        <property type="entry name" value="Primase_DnaG_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003108">
        <property type="entry name" value="PRK04031.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313:SF2">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56731">
        <property type="entry name" value="DNA primase core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0271">Exosome</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000240469" description="DNA primase DnaG">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="186"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00007"/>
        </source>
    </evidence>
    <sequence length="444" mass="49457" checksum="AE478FCE62F5F306" modified="2001-10-01" version="1">MVMLISLSLKKVIWMNVDPNLTKYMIKAKIYTDGVVEKPDVVGAIFGQTEGLLGDDLDLRDLQKSGKIGRIEVEIDSKKGRTEGYALIPSGLDQVETAILAAALETIDRIGPCKAKVEIANVEDVRVQKRQKIIERAKKIYQDMNSKGDDLSESLVKSVREEVEKSEIISYGEEKLPAGPAINDSDSIIVVEGRNDVLNLLKYGIKNTIAVQGTNIPETVKKLSKERTVTVFLDGDRGGDLILKEMLQVAEVDFVARAPPGTEVEELTYKQIVKALRYKTPIDQYLSMHGMNDELKEYSQRNNLVFTNHNSEQKKEVLEEPEAEKNEVLEQGEVTQQVSVPKEGYLDLLNPHDVSRRIEALSQLKTTEIYDSNGQRIASFPVAEAVDRIIQDPQGNTIVTGGIISQRLIDVAYNAGIKNIYGLKLGHITKKPVDINVIAWDRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CG2</accession>
    <name>Q97CG2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug-efflux transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0145846</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CG2"/>
    <dbReference type="PaxDb" id="273116-14324353"/>
    <dbReference type="KEGG" id="tvo:TVG0145846"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_10_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CG2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17472">
        <property type="entry name" value="MFS_YajR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517:SF2">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN MDTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="12"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="396" mass="43334" checksum="1B15EF2FE6AC47C5" modified="2001-10-01" version="1">MMRSFDRNQKRMIGAVAVIEVLRMFGIFLVVPVFTLYGKLFSNSPILIGIAFGAYGLTMALFQAPFGIISDRFGRKNVIILGMIPYIVGNLIAWHPVNIFGLIVGRLVAGSGAVTSSGMAMVQESVPPERRNLAMALLGIPIGFAFMIGIVLGPLISARLGADTLFLISALLGIISIIPMTAIKYHKPEYRAEERKKAGRIEPKTVLVGVVGFLVSLYMIVFFYYLPLYGTRAYGSHGYELLLFWSVVIGGIIAVASSSFADRGKTTLFSIIALILMLVSTPFVFLEPVATGNRDWFFIGTIIFFAGFSIYEIVFTPLVSKLSKKESYGANIGIYNTMQFLGQFAGGITGGVLVTLTLDYNSMLRTSMILAVLVLISLGFLYIPTKFREIRKKATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CJ1</accession>
    <name>Q97CJ1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system Cms protein Csm2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CRISPR type III A-associated protein Csm2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0115343</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This subunit may be involved in monitoring complementarity of crRNA and target RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm2 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053903.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CJ1"/>
    <dbReference type="STRING" id="273116.gene:9380878"/>
    <dbReference type="PaxDb" id="273116-14324324"/>
    <dbReference type="GeneID" id="1441597"/>
    <dbReference type="KEGG" id="tvo:TVG0115343"/>
    <dbReference type="eggNOG" id="arCOG06487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1648394_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09647">
        <property type="entry name" value="Csm2_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010149">
        <property type="entry name" value="CRISPR-assoc_prot_Csm2_III-A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01870">
        <property type="entry name" value="cas_TM1810_Csm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03750">
        <property type="entry name" value="Csm2_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="160" mass="19125" checksum="5AB6E571BFBB1B04" modified="2001-10-01" version="1">MMNSDRNKYRKENDMGKQVRDKEVQEIMDYIMNKGEDSEERVYKLFKMNGVIYEIACNISRNQNSETTQNQIRKFYDYTIKINSKDEKKAKIKLAMMMPQIYYAIQRKVISSDSPFVKFLEDSIDKLNKTEDFENAFNRFLNVFQAIVAYSKKSRSDRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CL0</accession>
    <name>Q97CL0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Vitamin B12-dependent ribonucleotide reductase</fullName>
            <ecNumber evidence="12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">TVG0096052</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ribonucleoside diphosphate reductase class-2 family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="12"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CL0"/>
    <dbReference type="STRING" id="273116.gene:9380859"/>
    <dbReference type="PaxDb" id="273116-14324305"/>
    <dbReference type="KEGG" id="tvo:TVG0096052"/>
    <dbReference type="eggNOG" id="arCOG04276">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_2_3_2"/>
    <dbReference type="OrthoDB" id="6188at2157"/>
    <dbReference type="PhylomeDB" id="Q97CL0"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02888">
        <property type="entry name" value="RNR_II_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050862">
        <property type="entry name" value="RdRp_reductase_class-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013344">
        <property type="entry name" value="RNR_NrdJ/NrdZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02504">
        <property type="entry name" value="NrdJ_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005010">
        <property type="entry name" value="PRK06406.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371:SF1">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371">
        <property type="entry name" value="VITAMIN B12-DEPENDENT RIBONUCLEOTIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="9 12" id="KW-0170">Cobalt</keyword>
    <keyword evidence="7" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="13">
        <location>
            <begin position="5"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB59233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="769" mass="86888" checksum="65D89AF2429BD391" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CL3</accession>
    <name>FTHS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formate--tetrahydrofolate ligase</fullName>
            <ecNumber evidence="1">6.3.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formyltetrahydrofolate synthetase</fullName>
            <shortName evidence="1">FHS</shortName>
            <shortName evidence="1">FTHFS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fhs</name>
        <name type="ordered locus">TV0088</name>
        <name type="ORF">TVG0090326</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.3" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916345.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CL3"/>
    <dbReference type="SMR" id="Q97CL3"/>
    <dbReference type="STRING" id="273116.gene:9380856"/>
    <dbReference type="PaxDb" id="273116-14324302"/>
    <dbReference type="GeneID" id="1441575"/>
    <dbReference type="KEGG" id="tvo:TVG0090326"/>
    <dbReference type="eggNOG" id="arCOG04541">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003601_3_3_2"/>
    <dbReference type="OrthoDB" id="53075at2157"/>
    <dbReference type="PhylomeDB" id="Q97CL3"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1510.10">
        <property type="entry name" value="Domain 2, N(10)-formyltetrahydrofolate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010030">
        <property type="entry name" value="PRK13505.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000199416" description="Formate--tetrahydrofolate ligase">
        <location>
            <begin position="1"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01543"/>
        </source>
    </evidence>
    <sequence length="536" mass="57913" checksum="5C560811AF6957D8" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CN2</accession>
    <name>Q97CN2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0070322</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="435" mass="48124" checksum="3E4E9F0B86631137" modified="2001-10-01" version="1">MQTSTYSKVRVNPWSVYPGAIAGWVMDAFNLSMMFLLVPVIADLFFPAHYGLAIIGTWSIYTTTFIFRPVGGLIFGRLGDRIGRKNTMTITLLGLGLITFATGFLPVYSVIGIAAPLLLFLFRIITGIFAGGEYGNGSSILMETVGAPKRGIWGAAIQSGYPLGYTLAAVIFLYLHYVFPGTEFAITGWRWMFWIGIIPAIIGLVIRLKMPESAMWIETKDRAKKLTLSDVFKNRRLTYEIITSVLAMTGIAWVYGLTLGFFPTVLSFDGFLKFPYFLYVVITAILVSLLGYLSSGYLSDHIGRRTVMVIYSILAIIFSIPLTFLIIKHYLGFSGSLIFASIIAFLTTGIYGVIPSFLSEKYPTEVRSTGTGIGFNGGFILGNWSTVFLLLIVSFTSPFFFGWWGIFVIIGELFIFSSALLSKETRGLDLSDVKL</sequence>
</entry>
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        <lineage>
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            <taxon>Thermoplasmata</taxon>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="Ccc1_fam"/>
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        <property type="entry name" value="Ferritin-like_SF"/>
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        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="326" mass="37440" checksum="EA25655F52990EB8" modified="2001-10-01" version="1">MQPDPDKWREFYRDELTDMNFYSKLSRTIKDDYLRENLSKLSEIEREHSEFWKENMKRFGIKYSNRYNRFKVTFLLLARYFLGNFLTARLLEHGEISTVSVYSDYLKKKDLDEDFRARLSKILDEEINHEEIFEKAMEKSQDTVERNKDVIYGISDGLVEVLAALAGLTSIIENNIDIALGGLVVGISGTASMTIGAYLSKKSETEYKLVEEEKRILFRRKKKDPEYVDKIKAESKTSALYVGLSYILGSAVPILPFVFLHKYLALILSIILVFIVQGIANAMVALSVNVRVMKMAIRASSLALLAAAITFFVGFAFHYFLHISII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CT2</accession>
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            <fullName evidence="12">DNA topoisomerase 1</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="12"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916276.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CT2"/>
    <dbReference type="STRING" id="273116.gene:9380784"/>
    <dbReference type="PaxDb" id="273116-14324233"/>
    <dbReference type="GeneID" id="1441504"/>
    <dbReference type="KEGG" id="tvo:TVG0017184"/>
    <dbReference type="eggNOG" id="arCOG01527">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002929_1_4_2"/>
    <dbReference type="OrthoDB" id="30963at2157"/>
    <dbReference type="PhylomeDB" id="Q97CT2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000003">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.65.10">
        <property type="entry name" value="Bacterial Topoisomerase I, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005555">
        <property type="entry name" value="PRK07220.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF26">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="Zn_ribbon_Top1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 12" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="13">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="14">
        <location>
            <begin position="157"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="12">
        <location>
            <begin position="195"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="12">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="12">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="12">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="12">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="12">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="12">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAB59161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="770" mass="87278" checksum="F2BD9842784F8FCC" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CU3</accession>
    <name>Y008_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-binding protein TV0008</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0008</name>
        <name type="ORF">TVG0008501</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054050.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CU3"/>
    <dbReference type="SMR" id="Q97CU3"/>
    <dbReference type="STRING" id="273116.gene:9380773"/>
    <dbReference type="PaxDb" id="273116-14324222"/>
    <dbReference type="GeneID" id="1441493"/>
    <dbReference type="KEGG" id="tvo:TVG0008501"/>
    <dbReference type="eggNOG" id="arCOG04179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122978_3_0_2"/>
    <dbReference type="OrthoDB" id="7912at2157"/>
    <dbReference type="PhylomeDB" id="Q97CU3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00026">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022889">
        <property type="entry name" value="DNA_bind_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003268">
        <property type="entry name" value="PRK04239.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000121568" description="DNA-binding protein TV0008">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="115" mass="13904" checksum="79E4A044ED6EB48B" modified="2001-10-01" version="1">MSDDEELEKIRRRQMEELQRQAMQRQMAEEEEKQREIEKARRQQILRQILDPSARERLNNVRLVRPDLADNVENQLIQLASMGRINRIIKESDIIDILSKLTENKREPKIERRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CV0</accession>
    <name>Q97CV0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTP1/OBG family GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0001192</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916258.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CV0"/>
    <dbReference type="STRING" id="273116.gene:9380766"/>
    <dbReference type="PaxDb" id="273116-14324215"/>
    <dbReference type="DNASU" id="1441505"/>
    <dbReference type="GeneID" id="1441505"/>
    <dbReference type="KEGG" id="tvo:TVG0001192"/>
    <dbReference type="eggNOG" id="arCOG00358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044997_0_0_2"/>
    <dbReference type="OrthoDB" id="372125at2157"/>
    <dbReference type="PhylomeDB" id="Q97CV0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="61"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="4">
        <location>
            <begin position="283"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="360" mass="40212" checksum="9FE381AB536E33E6" modified="2001-10-01" version="1">MSTIEERIKEIEDEIKRTQYNKATEHHIGLLKAKIARLQMEARAHKGGGHYGFSIPKSGDATVALVGFPNVGKSSLLNVLTNSESEVGSYAFTTLKAIPGVMEYKGAEIQILDLPGIIENASYGAGRGREILSTVRSADLIVIVTDVETGGLDKIVKELYNSGIVVNRRRKNIEIKKTTSGGIRVHKPKNVDIDSEYIRQLMKEFKLVNADVYIREQVNEDDIIEAVQKNYVYIPSIVAVNKIDMNGAFDTSPLTRFGEVFKVSAKNRIGIDELREGIYRSLKLLRVYLRNKAGVVDFDKPLILTDGSTVRDVCRKINRSLIDSFRYAIISGKNRPIGEQRVGIDYPLLDEDIVTIISRN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-14" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>P58232</accession>
    <name>RPO6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo6</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo6</name>
        <name evidence="1" type="synonym">rpoK</name>
        <name type="ordered locus">TV1249</name>
        <name type="ORF">TVG1288113</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917483.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58232"/>
    <dbReference type="SMR" id="P58232"/>
    <dbReference type="STRING" id="273116.gene:9382054"/>
    <dbReference type="PaxDb" id="273116-14325487"/>
    <dbReference type="GeneID" id="1441365"/>
    <dbReference type="KEGG" id="tvo:TVG1288113"/>
    <dbReference type="eggNOG" id="arCOG01268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112527_5_0_2"/>
    <dbReference type="OrthoDB" id="10567at2157"/>
    <dbReference type="PhylomeDB" id="P58232"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00192">
        <property type="entry name" value="RNApol_arch_Rpo6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000133826" description="DNA-directed RNA polymerase subunit Rpo6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00192"/>
        </source>
    </evidence>
    <sequence length="61" mass="6791" checksum="7FF38282DA063374" modified="2001-08-14" version="1">MKYTKFEKARIIGARALQIAMGAPVIIDIPKNVIDPVDIATIEFENNVIPITIKRVSKVLN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q977Y1</accession>
    <name>MOAC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="1">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaC</name>
        <name type="ordered locus">TV0760</name>
        <name type="ORF">TVG0764460</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaC family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917007.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q977Y1"/>
    <dbReference type="SMR" id="Q977Y1"/>
    <dbReference type="STRING" id="273116.gene:9381550"/>
    <dbReference type="PaxDb" id="273116-14324976"/>
    <dbReference type="GeneID" id="1441855"/>
    <dbReference type="KEGG" id="tvo:TVG0764460"/>
    <dbReference type="eggNOG" id="arCOG01530">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074693_1_2_2"/>
    <dbReference type="OrthoDB" id="10067at2157"/>
    <dbReference type="PhylomeDB" id="Q977Y1"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        </location>
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        <ligand>
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        <location>
            <begin position="104"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
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    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01224"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q977Y9</accession>
    <name>Q977Y9_THEVO</name>
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            <fullName evidence="4">Peroxiredoxin</fullName>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Suzuki M."/>
            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
            <strain evidence="2">GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="KEGG" id="tvo:TVG1313513"/>
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    <dbReference type="eggNOG" id="arCOG02064">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094970_2_0_2"/>
    <dbReference type="OrthoDB" id="69354at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13686">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60603.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="14794" checksum="C97E524B20961880" modified="2001-10-01" version="1">MSKTLSIVLASGTIDKIAAAGVITSGAVANGIDVNIFVTFWALMKFRKNDNTVNKLSYDGSEISDLVLSKMNEKKIQSGIEMIRNAKEVGNVHVYGCALMADVMGISKDDLDDMVEDIIGVGEFVSMTESSYTTLFI</sequence>
</entry>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Diverse_Substrate_Transporter"/>
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    </dbReference>
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        <property type="entry name" value="OS03G0707200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        <location>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="279"/>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="280" mass="30891" checksum="4C58A5F75E6588D8" modified="2001-10-01" version="1">MLSISTLVFVTFIWGVTFPIIKDVFLYLSPVNFLALRFAVSTLLFLPVVIKKIKHVKSEEWKAGLIAGTLLFIAYYLQTIGLLYTQPALSGTITGIYVVFVPIISYLYLRRRIAHVEVYSSAFAFIGLVLMSYSGLSNVSIELGDFMTLAAAVFYAMQLVYVSKHASHIDTFVFSFIQIAVAAFFSAVFIPTFPEPFKITIYSAFVVLFTAVFATFLATYVYVSALSKMNVTKVGVILIGEPIFADLTSVILYGEKIGPIALIGIIIMIASIGTISYFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q978B3</accession>
    <name>Q978B3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA invertase/resolvase family protein</fullName>
        </submittedName>
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        <name evidence="4" type="ORF">TVG1559049</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917734.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978B3"/>
    <dbReference type="STRING" id="273116.gene:9382318"/>
    <dbReference type="PaxDb" id="273116-14325743"/>
    <dbReference type="DNASU" id="1442194"/>
    <dbReference type="GeneID" id="1442194"/>
    <dbReference type="KEGG" id="tvo:TVG1559049"/>
    <dbReference type="eggNOG" id="arCOG03162">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_8_0_2"/>
    <dbReference type="OrthoDB" id="24728at2157"/>
    <dbReference type="PhylomeDB" id="Q978B3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03768">
        <property type="entry name" value="SR_ResInv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050639">
        <property type="entry name" value="SSR_resolvase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30461">
        <property type="entry name" value="DNA-INVERTASE FROM LAMBDOID PROPHAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30461:SF2">
        <property type="entry name" value="SERINE RECOMBINASE PINE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="3">
        <location>
            <begin position="3"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="201" mass="23000" checksum="38F5CF2E89D3B3D3" modified="2001-10-01" version="1">MTLAFAYVRASTIEEVRQGSNERQKEAIRQYTYQKGYELEIFEDKAKSGRNTLRPEFERMLKSLDRRPQIVIVSKIDRFARSLSDLLKTLEYLDQNGVGFVSVNDPGIDTTTPNGRLLLQILGAFAEFERNMINSRTKAGREQAQSKGVKFGRPPLKTRNGSFIDRRKVMELKQKGLSARAIAKSLECSTTPILRILRENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q978B8</accession>
    <name>Q978B8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1550441 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1550441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917728.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978B8"/>
    <dbReference type="PaxDb" id="273116-14325738"/>
    <dbReference type="GeneID" id="1442188"/>
    <dbReference type="KEGG" id="tvo:TVG1550441"/>
    <dbReference type="HOGENOM" id="CLU_2766328_0_0_2"/>
    <dbReference type="OrthoDB" id="55682at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="69" mass="7981" checksum="A31C22645B3A4F7A" modified="2001-10-01" version="1">MVSKHVHRYIIGGMAIFFIVYVLVANAYLSFRDFALYAALPSLVIMIALAFVYRYLENSIKFERRTGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q978D5</accession>
    <name>Q978D5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Formate hydrogenlyase subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1533303</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917713.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978D5"/>
    <dbReference type="STRING" id="273116.gene:9382296"/>
    <dbReference type="PaxDb" id="273116-14325721"/>
    <dbReference type="GeneID" id="1442173"/>
    <dbReference type="KEGG" id="tvo:TVG1533303"/>
    <dbReference type="eggNOG" id="arCOG01553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_5_1_2"/>
    <dbReference type="OrthoDB" id="5740at2157"/>
    <dbReference type="PhylomeDB" id="Q978D5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052375">
        <property type="entry name" value="Complex_I_20kDa-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="7">
        <location>
            <begin position="99"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="222" mass="24749" checksum="F611FBFDDAF7C272" modified="2001-10-01" version="1">MSSIWFLNGVKKGIKTERYPYAEPVSAPQWPSRLSGHDGGNCPVDAITPDGWVMEKCIFCRRCLPDYKPTGDQRIFTIKKTEDTFRRSFHVFPIDSGACGACNMEFLSIFSPQYDANRLGIFMVNTPRYADALIVMGVMTPGMKEALDRAYEAMPEPKLVIAMGACAVTGGIMGENPLDREKYNVEIAGCPPSPYTLIAALNAAMKDKRKADYFESKRQEVH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q978F3</accession>
    <name>PGMI_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional phosphoglucose/phosphomannose isomerase</fullName>
            <shortName evidence="2">Bifunctional PGI/PMI</shortName>
            <ecNumber evidence="2">5.3.1.8</ecNumber>
            <ecNumber evidence="2">5.3.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Glucose-6-phosphate isomerase</fullName>
            <shortName>GPI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Mannose-6-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV1464</name>
        <name type="ORF">TVG1514630</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Dual specificity isomerase that catalyzes the isomerization of both glucose-6-phosphate and mannose-6-phosphate to fructose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PGI/PMI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="2"/>
    <dbReference type="EC" id="5.3.1.9" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917694.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978F3"/>
    <dbReference type="SMR" id="Q978F3"/>
    <dbReference type="STRING" id="273116.gene:9382276"/>
    <dbReference type="PaxDb" id="273116-14325703"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_059687_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:carbohydrate derivative binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05017">
        <property type="entry name" value="SIS_PGI_PMI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019490">
        <property type="entry name" value="Glu6P/Mann6P_isomerase_C"/>
    </dbReference>
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        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035484">
        <property type="entry name" value="SIS_PGI/PMI_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02128">
        <property type="entry name" value="G6PI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006423">
        <property type="entry name" value="PRK08674.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10432">
        <property type="entry name" value="bact-PGI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000227798" description="Bifunctional phosphoglucose/phosphomannose isomerase">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="3">
        <location>
            <begin position="16"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>D-fructose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>D-fructose 6-phosphate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>D-fructose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>D-fructose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>D-fructose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>D-fructose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>D-fructose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>D-fructose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8ZWV0"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9HIC2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="304" mass="34558" checksum="09BD551B9A286EA6" modified="2001-10-01" version="1">MQFSEELLTLKDQVRFDKSFKVGKYDKIVIAGMGGSGIAGRIFSEIYDEKPVFLVDDYDIPSFVDDKTEFIAISYSGNTEETISASEEAAKKHANVHAITSGGSLSKMGFDTIIIPSGLQPRSSIGYLLMPLVNTFIKPKIGDVDEAYRLLSETDKDNEEEKSIASEIYNGEHIPVIYGSRPFRAIAYRWKTQFNENAKVLAYSNYFSELNHNDTMPLKDTYRKDEFYFLVFRSDDSRFSKRITVTEKITGNSFRMVDVRGSSLLAKLFYLIHFGDYLTYHLAELRGVDPQDVSLIEKLKKEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q978F4</accession>
    <name>Q978F4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NFACT RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1513619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="PaxDb" id="273116-14325702"/>
    <dbReference type="GeneID" id="1442153"/>
    <dbReference type="KEGG" id="tvo:TVG1513619"/>
    <dbReference type="eggNOG" id="arCOG01695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003612_2_1_2"/>
    <dbReference type="OrthoDB" id="10943at2157"/>
    <dbReference type="PhylomeDB" id="Q978F4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
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        <property type="entry name" value="RqcH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="406"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="364"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="604" mass="68699" checksum="C6350D084C6B231E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q978G8</accession>
    <name>Q978G8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1496158 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1496158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917678.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978G8"/>
    <dbReference type="STRING" id="273116.gene:9382259"/>
    <dbReference type="ESTHER" id="thevo-TV1447">
        <property type="family name" value="A85-Feruloyl-Esterase"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325686"/>
    <dbReference type="DNASU" id="1442136"/>
    <dbReference type="GeneID" id="1442136"/>
    <dbReference type="KEGG" id="tvo:TVG1496158"/>
    <dbReference type="eggNOG" id="arCOG05356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037618_0_1_2"/>
    <dbReference type="OrthoDB" id="39489at2157"/>
    <dbReference type="PhylomeDB" id="Q978G8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050583">
        <property type="entry name" value="Mycobacterial_A85_antigen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48098">
        <property type="entry name" value="ENTEROCHELIN ESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48098:SF3">
        <property type="entry name" value="IRON(III) ENTEROBACTIN ESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="313" mass="35905" checksum="1F1FB3599F82A119" modified="2001-10-01" version="1">MKTEIVEIESECLKDNFLKDDWKRQILVYRPDDAEGGLPLFIELAGTNWRPRTHNKFSLIIEKLFSSGLKAVVLNPNFSTRYNMNQYLNSPAVGSYEDFIVKELIPYFKDKYGTDHTALFGKSSGGFGSYTLAARHPDIIDGFADHFGDSCFFYLYADDMAAAFRYIQRHGKSESVKMLTSHMPSDDEMKVLNVIGSSAFYSPDMNDVNGFDLPFDEDTGELIEDVWQRWVQFDPAKNVLKYSGALKKMKAIYLDVGTNDEYKLYIGTRALHKKLGLANIDHYYEEFNGGHFGNSERYLYSLPFLYRKLNDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q978G9</accession>
    <name>Q978G9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1494892 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1494892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978G9"/>
    <dbReference type="STRING" id="273116.gene:9382258"/>
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    <dbReference type="KEGG" id="tvo:TVG1494892"/>
    <dbReference type="eggNOG" id="arCOG00132">
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        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0022857">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
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        <property type="entry name" value="MFS general substrate transporter"/>
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        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
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        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
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        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
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        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
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            <end position="183"/>
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            <end position="228"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="381" mass="42317" checksum="EFF4E857CCCB9387" modified="2001-10-01" version="1">MKPASLLISISKAARSFIFSMMAIITPYYLQSMHISALYVGIIIFISLVSGSVFILLYSRVPFSAKNRVLLLSVLMVAALTVLVFVRNVVIFVVAVFIGGISLAGRDFSAYPPIEKFAISSFETIQKDKNEAFSVYNFGSYGSAAIASAFLYLDLRYSFQEIFEILLVLSILQLILYIFVKLPDYKRESVNLRLPKNVKAHVNTLSYLFAIDAFGGGFVTVSMLTLWFKAVYHISLSEAGFIFIIVNVLTALSILVSSKISGKIGLIKTMVFTHIVSNFFLILMPLVPNLYAAELFLYLRQTTSQMDVPARDSFTNTIIPPGDRIKTNSVFTTVRTLGHGPGPAIGGYLIEAGPPSLLFVAGGVKILYDLLLYYKYHWFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>Q978I0</accession>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60577.1"/>
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    <dbReference type="GeneID" id="42990964"/>
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    <dbReference type="OrthoDB" id="56402at2157"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="55" mass="6374" checksum="DCCF7F9A8EBC0C12" modified="2001-10-01" version="1">MDEKEKLLEIVKVNSQKNTVRITIPRRIAKILDIGDGDFIGYYEAGRTIIIRKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q978I7</accession>
    <name>Q978I7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1473494</name>
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    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily. ABHD14 family.</text>
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        <property type="protein sequence ID" value="BAB60570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978I7"/>
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    </dbReference>
    <dbReference type="PaxDb" id="273116-14325667"/>
    <dbReference type="KEGG" id="tvo:TVG1473494"/>
    <dbReference type="eggNOG" id="arCOG01648">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_28_1_2"/>
    <dbReference type="PhylomeDB" id="Q978I7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46197:SF3">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="19"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="134"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="196" mass="21956" checksum="7854F4E17A18489F" modified="2001-10-01" version="1">MHGKRVFHRYLKSGAKKDIVMLHGWSFTSRDWETPGLFNEYANLSFNVYAPDYPGFGMSEPSDFYSVKRGNIEASASFIKDYMQSLGVEHAVILGASMGGGMAILMGMAHPDMVDAVIAVAPAWVENYTEKMKNIEKPVLLVWGSEDTVVDPHFGNKYRSAIKNSQLEIVEGSKHPVYLEKPKRFIEITTGFLKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q978J4</accession>
    <name>Q978J4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Hypohtetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">TVG1467238</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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        </source>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
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    </evidence>
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        <source>
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            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="659" mass="74153" checksum="A8B301AE36FBA4E9" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
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    <organism evidence="2 3">
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            <taxon>Thermoplasmata</taxon>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="44228" checksum="7E4CB5CB11ABF147" modified="2001-10-01" version="1">MRNITENDRIFLIEFSIVLLTFFSLSVYAGSFTFVLIEGSIPLYYLGVYFSVAIIFYLAFNPVSYEYVKRYGDLFSVPISILVLSVAFIIIYISQGAVSITASLIIFTISFTVFYNSTVKNGYRRDINESSFIISSGIGILLGLLVSAFFGGVVSDLYSYVSILLLMIGLITIILFTKKTNSKPEKMDILYIFMRPFYSMDKVRKADKKLLLLADSLDEIFLWLAIGASFPFLIAAGIGSGLSHSAVMISIATASFVAAVAVYLYLSMQLPIMSFYYLSKDVLLIVGLLLMSFMSPPSYFASLAFLSFFPAAFIGSEKFLKSQFPADLNYREITAFFRNPIMVISPLMGSILWIVGRHMLFLISVGFAVMALVTGILVITNPRLFKLSGAEREKDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q978K6</accession>
    <name>Q978K6_THEVO</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <lineage>
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            <taxon>Thermoplasmatales</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="DNASU" id="1442099"/>
    <dbReference type="KEGG" id="tvo:TVG1455840"/>
    <dbReference type="eggNOG" id="arCOG00446">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_2_2_2"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF20">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN, SUBUNIT BETA (ETFB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="1">
        <location>
            <begin position="37"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="260" mass="28196" checksum="5230276BBC5A7635" modified="2001-10-01" version="1">MRLRNVYTLSTNTGIMVNVIVLIKQIIDIDQMKTDSEGKPILSGIPYRVENLSKNAIEAAVQLKEKNGGKVTGIIFGTEASTAAMKEAYAMGVDEGIIITGYKGSNPIVTAKAITSILKNLQYDIVVLGDQSADSYTGMLPGLLSAMTGFPLLGNANSITINGRSVRITQVGEAENAEIEADMPAIVSVTQEINQPRLPPVLQIMAAGRKPIRMEKYDGSFSNVNVISNLAPKSERKKKIFEKVDEGIPEIVKVIKEEMR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q978K9</accession>
    <name>TRUD_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable tRNA pseudouridine synthase D</fullName>
            <ecNumber evidence="1">5.4.99.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(13) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truD</name>
        <name type="ordered locus">TV1406</name>
        <name type="ORF">TVG1453468</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(13) in tRNA = pseudouridine(13) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42540"/>
            <dbReference type="Rhea" id="RHEA-COMP:10105"/>
            <dbReference type="Rhea" id="RHEA-COMP:10106"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.27" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917638.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978K9"/>
    <dbReference type="SMR" id="Q978K9"/>
    <dbReference type="STRING" id="273116.gene:9382215"/>
    <dbReference type="PaxDb" id="273116-14325645"/>
    <dbReference type="GeneID" id="1442096"/>
    <dbReference type="KEGG" id="tvo:TVG1453468"/>
    <dbReference type="eggNOG" id="arCOG04252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005281_4_1_2"/>
    <dbReference type="OrthoDB" id="1798at2157"/>
    <dbReference type="PhylomeDB" id="Q978K9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160150">
        <property type="term" value="F:tRNA pseudouridine(13) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01082">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000152557" description="Probable tRNA pseudouridine synthase D">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="TRUD" evidence="1">
        <location>
            <begin position="146"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01082"/>
        </source>
    </evidence>
    <sequence length="409" mass="47352" checksum="D88E3EEEE5609452" modified="2001-10-01" version="1">MNKPENFEEAIEIKRDPEDFSVEEIADIEPDPNGKYTIIKARVRDWDTNRIAAEIARRLHMSRKRVTFAGTKDKRAVKLQYFCINSADVDVASLSGIKDFEVIESFKSSHYLTLGDLIANHFKIRFYGIDPEMFRERYVHIISKGGFPNFFGDQRFGSRRRNTHEIGKLIIKGEYEEAVKKYIYDEKYDKESYRKHFIDTLDYKTALERFPHSLSFERSLIGYYARNGTFKGAFDSLPKNLTIMFVHAYQSYLFNRILDERLKIYGLNAVLPGDIAFPVDAYFNPDKSKPIEVNSYNREKISKLVSSDKIRISLPIFGYKTWIDNSDFGDVEYGILKEEGISQDDFKNKDFAYLSSSGDRRIISAKPINFSLENNVVEFTLGKGIYATVFLSSIGRLKENVYSDSEAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q978U4</accession>
    <name>Q978U4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="13">TC-AMP synthase</shortName>
            <ecNumber evidence="3 13">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">TVG1360240</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3 13"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917556.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978U4"/>
    <dbReference type="STRING" id="273116.gene:9382129"/>
    <dbReference type="PaxDb" id="273116-14325560"/>
    <dbReference type="GeneID" id="1441438"/>
    <dbReference type="KEGG" id="tvo:TVG1360240"/>
    <dbReference type="eggNOG" id="arCOG01952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_0_1_2"/>
    <dbReference type="OrthoDB" id="39992at2157"/>
    <dbReference type="PhylomeDB" id="Q978U4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010923">
        <property type="entry name" value="T(6)A37_SUA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="Sua5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004930">
        <property type="entry name" value="Tln_factor_SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="15">
        <location>
            <begin position="14"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR004930-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAB60463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="361" mass="39805" checksum="78665735A75E9958" modified="2001-10-01" version="1">MTRVIHIDRNSYSKDDLYYAVEALKHGGTVVFPTETVYGLGANAMNDEACKKIFIAKGRPSDNPLIVHISDMDQLENFSKNVKEEYKAILKELWPGPLTVVVEKANGIPDTVTAKLPTVAIRMPDDSIARNLIDMAGPIAAPSANISTKPSITDSKDAIRFLDGRVDVIIDAGKVKFGIESTIIDLTQDPVRLLRPGAFSVEDLEPLFGKITVDKVALGKAEAKVAITPGMKYRHYSPNKKIFLCKDREVVESLKEELPNNGVLMCTEEVSQGISTNKIIVGRDSDLYSVAHNLFSSFIELDDSSYNYGVIIPFEEKGIGLAIMNRIRKASTAVISSSEELKEYLDEYGISDNTKEIKDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q978X0</accession>
    <name>Q978X0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH hxlR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1336486</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2008-06" db="PDB data bank">
            <title>The crystal structure of a possible HxlR family transcriptional factor from Thermoplasma volcanium GSS1.</title>
            <authorList>
                <person name="Tan K."/>
                <person name="Tesar C."/>
                <person name="Moy S."/>
                <person name="Joachimiak A."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 10-117</scope>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_156769120.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="3DF8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.65 A"/>
        <property type="chains" value="A=10-117"/>
    </dbReference>
    <dbReference type="PDBsum" id="3DF8"/>
    <dbReference type="AlphaFoldDB" id="Q978X0"/>
    <dbReference type="SMR" id="Q978X0"/>
    <dbReference type="STRING" id="273116.gene:9382102"/>
    <dbReference type="PaxDb" id="273116-14325534"/>
    <dbReference type="GeneID" id="1441412"/>
    <dbReference type="KEGG" id="tvo:TVG1336486"/>
    <dbReference type="eggNOG" id="arCOG01057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_5_2_2"/>
    <dbReference type="OrthoDB" id="56199at2157"/>
    <dbReference type="PhylomeDB" id="Q978X0"/>
    <dbReference type="EvolutionaryTrace" id="Q978X0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204:SF18">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="7" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="4">
        <location>
            <begin position="19"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="3DF8"/>
        </source>
    </evidence>
    <sequence length="117" mass="13067" checksum="EE309C7E43529DA4" modified="2001-10-01" version="1">MDQTRSKACMLRYGDTEICIDPSESVLHLLGKKYTMLIISVLGNGSTRQNFNDIRSSIPGISSTILSRRIKDLIDSGLVERRSGQITTYALTEKGMNVRNSLMPLLQYISVLDRNGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q978X9</accession>
    <name>Q978X9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1326649</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760275.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978X9"/>
    <dbReference type="STRING" id="273116.gene:9382092"/>
    <dbReference type="PaxDb" id="273116-14325524"/>
    <dbReference type="GeneID" id="1441402"/>
    <dbReference type="KEGG" id="tvo:TVG1326649"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_16_1_2"/>
    <dbReference type="PhylomeDB" id="Q978X9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="456"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="518" mass="55632" checksum="AB64C6C5C994617B" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Y2</accession>
    <name>Q978Y2_THEVO</name>
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        <recommendedName>
            <fullName evidence="5">DUF35 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">TVG1322512</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Y2"/>
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    <dbReference type="PaxDb" id="273116-14325521"/>
    <dbReference type="DNASU" id="1441399"/>
    <dbReference type="GeneID" id="1441399"/>
    <dbReference type="KEGG" id="tvo:TVG1322512"/>
    <dbReference type="eggNOG" id="arCOG01287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1615398_0_0_2"/>
    <dbReference type="OrthoDB" id="9573at2157"/>
    <dbReference type="PhylomeDB" id="Q978Y2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
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        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF4">
        <property type="entry name" value="DUF35 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="47"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="81"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18904" checksum="42645C9E858108BE" modified="2001-10-01" version="1">MTINPNAKLPEDESGTIVFSTDPLVIKYHYEIDYIHSYAQDSEFFRALGKHKLMGSKCKKCGYVYATPRAHCMMCGSETEWYELPLKGKVHTFTTCYFSGEEFLNETPFNLIMVEFEGVNSLFMSRLIGADTEDIYIGMPVKAKFRRNQKMNVTDVYFVPDVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q979A0</accession>
    <name>Q979A0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1300339</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760272.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979A0"/>
    <dbReference type="STRING" id="273116.gene:9382067"/>
    <dbReference type="PaxDb" id="273116-14325500"/>
    <dbReference type="GeneID" id="1441378"/>
    <dbReference type="KEGG" id="tvo:TVG1300339"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_16_0_2"/>
    <dbReference type="PhylomeDB" id="Q979A0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="511"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="561" mass="60809" checksum="77DE3845B0A89004" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-24" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q979D0</accession>
    <name>RS15_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps15</name>
        <name type="ordered locus">TV1231</name>
        <name type="ORF">TVG1268344</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917466.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979D0"/>
    <dbReference type="SMR" id="Q979D0"/>
    <dbReference type="STRING" id="273116.gene:9382036"/>
    <dbReference type="PaxDb" id="273116-14325469"/>
    <dbReference type="GeneID" id="1441348"/>
    <dbReference type="KEGG" id="tvo:TVG1268344"/>
    <dbReference type="eggNOG" id="arCOG04185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090139_2_0_2"/>
    <dbReference type="OrthoDB" id="6533at2157"/>
    <dbReference type="PhylomeDB" id="Q979D0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_uS15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000115624" description="Small ribosomal subunit protein uS15">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="145" mass="16750" checksum="62DF29C90A6D4044" modified="2001-10-01" version="1">MARMHTRKRGKSGSKKVYGVEPNWVQYSKDEILNIAVDLKKSGMTSSVVGIRLRDQYGVPTVKAIFGKKLNKLLAEKGIRDEIPEDLNSLIKRYNNVAKHVELNPKDHSNKRGRDLIMAKMLRLVRYYKRTGVLDQKWNLSKVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q979D2</accession>
    <name>Q979D2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1267449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917464.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979D2"/>
    <dbReference type="STRING" id="273116.gene:9382034"/>
    <dbReference type="PaxDb" id="273116-14325467"/>
    <dbReference type="DNASU" id="1441346"/>
    <dbReference type="GeneID" id="1441346"/>
    <dbReference type="KEGG" id="tvo:TVG1267449"/>
    <dbReference type="eggNOG" id="arCOG01065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_1_1_2"/>
    <dbReference type="PhylomeDB" id="Q979D2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="411" mass="45258" checksum="FB9C8581EE484C1B" modified="2001-10-01" version="1">MMTNPKVIILGGRFGGLQTAYELKRYLKGKIDIKLIERNRYIYFRPALPHVAIGLENPDDLRIDISEALSAKGIEYIQGTVTRIDPAKNTVEYNKEGAGPSKEKYDYLVIALGSHLATELITGFEKFGSSVCEADLALSMWNKLNAFKGGNITLGSAKFIQDNKNRPTNNPDAYAPLLESACEGPVYEMSIMLYDYFKAKGMLDKVKMTVYSPGKYLSDLSETNRATVAAMYKGMDIDIVDNFVLKEVTEKEVISEDGKRLPSDLSFILPPYMGQQMVKDAGLGDEAGFVLTDTNMKSVKYDNVYAVGDINALIVPKMAALAVKSARIAAANIANNLGNKVPIEVYDPKIVCIADDPYGKYAVAIVDSTAYGGNISEAVPSAANHLKKSLFTRYFLWSKGDLAMDKYLTSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q979D3</accession>
    <name>Q979D3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1266004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917463.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979D3"/>
    <dbReference type="SMR" id="Q979D3"/>
    <dbReference type="STRING" id="273116.gene:9382033"/>
    <dbReference type="PaxDb" id="273116-14325466"/>
    <dbReference type="DNASU" id="1441345"/>
    <dbReference type="GeneID" id="1441345"/>
    <dbReference type="KEGG" id="tvo:TVG1266004"/>
    <dbReference type="eggNOG" id="arCOG04215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061573_1_0_2"/>
    <dbReference type="OrthoDB" id="31228at2157"/>
    <dbReference type="PhylomeDB" id="Q979D3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038764">
        <property type="entry name" value="GNAT_N_AcTrfase_prd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41700">
        <property type="entry name" value="GCN5-RELATED N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41700:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="263" mass="30497" checksum="7EA47EF459A7EF93" modified="2001-10-01" version="1">MKIVRIYDTADLDDVLGVIKSAWNSQNVNGAFKDTIASMRYHGGIILGAYSNDRLVGMSFSYPGYRHGSVYLYSHMTGVIEEEKHRNIGYELKMKQREVARSMGYELIAWTFDPANPLNAYFNLSKLKCISRTYVKNFYGRMEDGINRGMRTDRLVAEWWLNIDLDVDYDNVVFINSGKEGNYKPIEYSNSDVLGFKMPNSINYFSENIERGNNLKDIIEKVFNTLFEKGYSIVDYKRGEDNYFVFKKIENIPSRIFDSNIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q979E8</accession>
    <name>Q979E8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cas12f1-like TNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1244656</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979E8"/>
    <dbReference type="SMR" id="Q979E8"/>
    <dbReference type="STRING" id="273116.gene:9382016"/>
    <dbReference type="PaxDb" id="273116-14325451"/>
    <dbReference type="DNASU" id="1441327"/>
    <dbReference type="KEGG" id="tvo:TVG1244656"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_791354_0_0_2"/>
    <dbReference type="OrthoDB" id="57553at2157"/>
    <dbReference type="PhylomeDB" id="Q979E8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="288"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="393" mass="45363" checksum="C51E79B3992F6C45" modified="2001-10-01" version="1">MIVVYVFVYAFKAVKQYFYPSDNLKVLMHKFTDMVNFTINIMIEKNLTSRNSVSNEVYHKLKEYEIPSYYYIEAINKAVALVKTYRKRLKKKQKATVPHVEKPMLSTYYGFKIVGGNIMIPIANREYESIPLNAYVRDATSMVKVHSFALSAYTLSLTIGRDVDAIECTTTVGVDRNLRNITVGNEFHHEIYDLSNIVEIKQRYRKKESHFHRNDRRIREKILSKYGKRERNRVKQIMHETSKAIVSNASTKKEMIVLENIIGINQITKKGDGKGKDYRFLLKNAFPYGMLASQVMYKASWEGLPVIELTRKETRNTSRMCSVCGSLTRIEHGRILKCDSCGLEIDRDVNACINIAFRYRTCLKRSHKGLPGEAVKQSKDVEQMAGSQISMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q979M6</accession>
    <name>Q979M6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH dehydrogenase I chain G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1162541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917368.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979M6"/>
    <dbReference type="STRING" id="273116.gene:9381933"/>
    <dbReference type="PaxDb" id="273116-14325372"/>
    <dbReference type="GeneID" id="1441250"/>
    <dbReference type="KEGG" id="tvo:TVG1162541"/>
    <dbReference type="eggNOG" id="arCOG04654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193440_0_0_2"/>
    <dbReference type="OrthoDB" id="57027at2157"/>
    <dbReference type="PhylomeDB" id="Q979M6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="entry name" value="NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001457">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005023">
        <property type="entry name" value="PRK06433.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33269">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33269:SF17">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00499">
        <property type="entry name" value="Oxidored_q3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="82" mass="9039" checksum="FA97DE7507A29966" modified="2001-10-01" version="1">MIYTLIFSIIAVFLIAFSVLSVKEKNITHSAIYLFLFLFILAAEFLFLGASFVAAVEILVYIGAVVTLIVFTVMLTGGKEIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q979P7</accession>
    <name>TF2B2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription initiation factor IIB 2</fullName>
            <shortName evidence="1">TFIIB 2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tfbB</name>
        <name type="ordered locus">TV1113</name>
        <name type="ORF">TVG1144753</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917347.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979P7"/>
    <dbReference type="SMR" id="Q979P7"/>
    <dbReference type="STRING" id="273116.gene:9381912"/>
    <dbReference type="PaxDb" id="273116-14325351"/>
    <dbReference type="GeneID" id="1441229"/>
    <dbReference type="KEGG" id="tvo:TVG1144753"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="PhylomeDB" id="Q979P7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000119341" description="Transcription initiation factor IIB 2">
        <location>
            <begin position="1"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="130"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="224"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TFIIB-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <sequence length="313" mass="35203" checksum="09CAB21F626CDA89" modified="2001-10-01" version="1">MPYSDKMAIESEAPKRCPECHSEHLIRDYEHGELICADCGAVIEDSFIDQGPEWRAFDSDQDERRARTGSPMTYLSHDKGLATEISWSNKDYYGKRIPHKNRAQIYRVRKWHQRIRVSNAAERNLSLALQLLNDIGAKLGIPKDIKETSALIYRKAVEKNLIRGRSIESIVCASIYAACRKVNIPRTLDEIAKASEVNKKKIGKAYRHLAKELDLNLRPTTPFSYIAQFCNKLDLDKQAIVISEDIVRQAMSMGISSGKGPTGIAAAAIYIASVKVGKPRTQKEIARISGVTEVTIRNRYKEISKALNISISE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q979R4</accession>
    <name>TRUA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="1">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truA</name>
        <name type="ordered locus">TV1096</name>
        <name type="ORF">TVG1128771</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979R4"/>
    <dbReference type="SMR" id="Q979R4"/>
    <dbReference type="STRING" id="273116.gene:9381893"/>
    <dbReference type="PaxDb" id="273116-14325334"/>
    <dbReference type="KEGG" id="tvo:TVG1128771"/>
    <dbReference type="eggNOG" id="arCOG04449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_4_2_2"/>
    <dbReference type="PhylomeDB" id="Q979R4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160147">
        <property type="term" value="F:tRNA pseudouridine(38-40) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00497">
        <property type="entry name" value="PseudoU_synth_TruA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000057516" description="tRNA pseudouridine synthase A">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <sequence length="246" mass="28353" checksum="124AECE9BBED1218" modified="2001-10-01" version="1">MTHAYFFKFGYIGYMFTGYQRGNGVKSVEDTIIKALNLNSPSLIKTAARTDRGVSALSNVFYIETERRPQDIAGIINSKNEYVFVHSFAEVEHGRNPRYCDTKTYMYMLPGETECDSLMVTASGFLGFHDFSKFSKKDTRNPWRSIDEVKCHRESFGVVLEFSAKSFLWNQVRRMLAFILEFRGNKIDPFSVSNIYSHIAPPENLILKDIVYKDLSFKPLNNGLKRQRLMMEKSLISYIVLKNVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q979R7</accession>
    <name>Q979R7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1123172 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1123172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917325.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979R7"/>
    <dbReference type="STRING" id="273116.gene:9381890"/>
    <dbReference type="PaxDb" id="273116-14325331"/>
    <dbReference type="GeneID" id="1441207"/>
    <dbReference type="KEGG" id="tvo:TVG1123172"/>
    <dbReference type="eggNOG" id="arCOG07397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121285_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="193" mass="22146" checksum="4216CADF0B1FB646" modified="2001-10-01" version="1">MAEEEKSDTKKDQINFDSVPKKKRSVFSFIKKKPKEEVKTVIKVKTVGDVPVIVKARKKITEGDIKSAIIDSYNSVKDDYVKYFGLSVNYKGNRDFIINVLSSLGIKLSEEAYVDGKLIRQRLDSLDLGNSEPRLACFAKLSEFYLDFYEVAKYSDKDIENTEKILEDLMGIYNYMDVAKLYFEKEGSENVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q979U2</accession>
    <name>Q979U2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1094530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917297.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979U2"/>
    <dbReference type="STRING" id="273116.gene:9381863"/>
    <dbReference type="PaxDb" id="273116-14325306"/>
    <dbReference type="GeneID" id="1441179"/>
    <dbReference type="KEGG" id="tvo:TVG1094530"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="PhylomeDB" id="Q979U2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="306" mass="34288" checksum="0F271E20D40722DC" modified="2001-10-01" version="1">MCAYYIECRDISKKYVKGPEALKSISFKVPASGILSLIGRNGAGKTTLIRILSTELLPSSGYAEIDGIDIIGEPDEIRKRIAIIPQEARAISWLTPIQTITTFLLYRGINYSEAKRRSMEVLKKLELEQIAGKLNRTLSGGQKRKVLVAALIASDADILFMDEPTTGLDPISRAELWDLLAELKREKFIFLTTHYLEEAERLSDRIAILDSGELKGIGTISELRSRLPKKYSINLIQTSNLDGEIEINGTVLKTGRRLFIDEEEAYSIVRDLINKNAKFSINPISLEDIYYSIAKRDIGGDAYVQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q979V9</accession>
    <name>TRPC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Indole-3-glycerol phosphate synthase</fullName>
            <shortName evidence="1">IGPS</shortName>
            <ecNumber evidence="1">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpC</name>
        <name type="ordered locus">TV1051</name>
        <name type="ORF">TVG1077942</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917280.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979V9"/>
    <dbReference type="SMR" id="Q979V9"/>
    <dbReference type="STRING" id="273116.gene:9381845"/>
    <dbReference type="PaxDb" id="273116-14325289"/>
    <dbReference type="GeneID" id="1441162"/>
    <dbReference type="KEGG" id="tvo:TVG1077942"/>
    <dbReference type="eggNOG" id="arCOG01088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034247_0_1_2"/>
    <dbReference type="OrthoDB" id="15223at2157"/>
    <dbReference type="PhylomeDB" id="Q979V9"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_A">
        <property type="entry name" value="IGPS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001374">
        <property type="entry name" value="PRK00278.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854:SF2">
        <property type="entry name" value="INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154305" description="Indole-3-glycerol phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00134"/>
        </source>
    </evidence>
    <sequence length="237" mass="27288" checksum="241693CF69129F1E" modified="2001-10-01" version="1">MNYDSVYKSNKLRSFPYSRRRGIISLKERIIELNRSGKRGVIAEYKRRSPSGLKVDYSIEDYLSYVMEHRIAGLSILTEPSFFNGSFQDVVVAHRYNIPILVKDFVPDSEFVDYGFNAGGDAVLVILDFLDYESIKRIVERAADLGMDVLEEFHNQDALKRQYIRDNVMIGYNRRDLTNLLMDEKIPDFSGEVRILESGISIDNVKNLDLKFQGYLIGTSILKKDGTLEYLEMEGII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-15" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q979W4</accession>
    <name>NADE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="1">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadE</name>
        <name type="ordered locus">TV1046</name>
        <name type="ORF">TVG1071522</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917275.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979W4"/>
    <dbReference type="SMR" id="Q979W4"/>
    <dbReference type="STRING" id="273116.gene:9381840"/>
    <dbReference type="PaxDb" id="273116-14325284"/>
    <dbReference type="GeneID" id="1441157"/>
    <dbReference type="KEGG" id="tvo:TVG1071522"/>
    <dbReference type="eggNOG" id="arCOG00069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059327_1_1_2"/>
    <dbReference type="OrthoDB" id="39312at2157"/>
    <dbReference type="PhylomeDB" id="Q979W4"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010587">
        <property type="entry name" value="PRK13980.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152238" description="NH(3)-dependent NAD(+) synthetase">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="231"/>
            <end position="232"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <sequence length="242" mass="27227" checksum="5AB522917BA85C88" modified="2001-10-01" version="1">MPDYQKEIERIGEFLRKIIADRKAVIGISGGIDSSVTLGILSKFFLHENIKAVFMPDATTPKSDYDDVDVLASTFGVKYSTVNIQNIVDTFSKTLSAVDKGAIGNIKSRIRMIVLYYFANIYNGIVVGTTNRTELLLGYYTKYGDGGCDVEPIEHLYKRDIYEIARLLGVPESIIKKKPTAGLWQGQTDEDEIGMPYSKIDDILSSIFDKGELREDPDFKKILDLHSKSDHKRRLPYSLSPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q979W6</accession>
    <name>Q979W6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flagella-related protein I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1070228</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917273.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979W6"/>
    <dbReference type="STRING" id="273116.gene:9381838"/>
    <dbReference type="PaxDb" id="273116-14325282"/>
    <dbReference type="GeneID" id="1441155"/>
    <dbReference type="KEGG" id="tvo:TVG1070228"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_2_2_2"/>
    <dbReference type="OrthoDB" id="33500at2157"/>
    <dbReference type="PhylomeDB" id="Q979W6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF6">
        <property type="entry name" value="TYPE IV PILUS RETRACTATION ATPASE PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="143"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="410" mass="46237" checksum="B57AD7D9F4549AE7" modified="2001-10-01" version="1">MRVYTVDNKFNEETVKLGLLYLSQAHEVSMENFVKQYALDQRMASSVSEFAEAYRIGMGKVEPIINDPMVEDITITSENYVYIYHSKLGPMRTDVFFTEDETRKFAQHIAQRSGKTVSVYNPVVDVSYKNYRINIVYEDVGKPSISIRKLRAAAFTPFDLIQSGAATPKVMAYLWLAIEHRSNIIITGSTSSGKTTLLNAILSFVPRASRIVSIEDARELNIMNENVVSLQSKISLTQSGGISVNDLIEFSKYDGADYLVLGEARGDVIRSYFRYITSGRNAITTIHGGSSSALIRRLKSLPLYLPISNILAIDAIVEVKNSGRRYINKISEPFYEGSLSLNNVFDYSSGHYEESGVSYLLENIASKTGMDREYIIKDLDRKAKILENMQKPADYMDFVRKVRDEANKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q979X6</accession>
    <name>Q979X6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1059341</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917263.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979X6"/>
    <dbReference type="STRING" id="273116.gene:9381827"/>
    <dbReference type="PaxDb" id="273116-14325272"/>
    <dbReference type="GeneID" id="1441145"/>
    <dbReference type="KEGG" id="tvo:TVG1059341"/>
    <dbReference type="eggNOG" id="arCOG01130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="PhylomeDB" id="Q979X6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000033">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="30"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="381" mass="42976" checksum="F7DB4E4F88CB7393" modified="2001-10-01" version="1">MLSQRLQYVNESATVSASNYVEKLKKQGIKVYNFGIGEPDFTTPQHIIEYAFEMAKEGKTHYTPSNGIHELREKVSEKLKNRNNINASPDEVLITPTKFGINLAMMVILNPGDEVLIPEPYYVSYPDIVRLAGGKPVTVSTLEDYSLDFDLMRKYVTPKTKAIIFNNPTNPTGKVYDEKEIKSLVDFALEYGLYIVSDEIYEDLIYNGKLISPASYSEMWGKSITLNGFSKGYAMTGWRIGYMAAPREIVEAANVIQQQTITCVSSISQYAALRALDDTESPKKMKDEFKRRRDLILSILDSSDKLNVTEPDGAFYVFPEYNSDISSNKVSEDLLEKYQVVVTPGSAFGRQGEHHFRISFATSEDIIKEGAERIIKFFASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A02</accession>
    <name>Q97A02_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Iron-sulfur protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1030250</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917236.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A02"/>
    <dbReference type="STRING" id="273116.gene:9381801"/>
    <dbReference type="PaxDb" id="273116-14325246"/>
    <dbReference type="GeneID" id="1441118"/>
    <dbReference type="KEGG" id="tvo:TVG1030250"/>
    <dbReference type="eggNOG" id="arCOG02854">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_4_2"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="PhylomeDB" id="Q97A02"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="4"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="114" mass="12624" checksum="6620818A7EA45018" modified="2001-10-01" version="1">MVFRKVSISTKIFEKTNSVVTWIAHKPVLLTKYNGDYFAMEAVCGHMGCAILNKVEGKEAVCPAHGARYDVTNGNKVSEPQIKPEAPCEYDSIGEPLKTFKVLENNGFLEVDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A27</accession>
    <name>Q97A27_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">N-acetylglucosaminyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1005578</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760260.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A27"/>
    <dbReference type="STRING" id="273116.gene:9381775"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325200"/>
    <dbReference type="GeneID" id="1442060"/>
    <dbReference type="KEGG" id="tvo:TVG1005578"/>
    <dbReference type="eggNOG" id="arCOG01389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_659913_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97A27"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050501">
        <property type="term" value="F:hyaluronan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085029">
        <property type="term" value="P:extracellular matrix assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030213">
        <property type="term" value="P:hyaluronan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22913">
        <property type="entry name" value="HYALURONAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22913:SF12">
        <property type="entry name" value="MANNURONAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13641">
        <property type="entry name" value="Glyco_tranf_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="376" mass="43248" checksum="9BA96A293FFEC7BC" modified="2001-10-01" version="1">MTIRYSTKDVTIVVPVYNEEVGIFAETMKSLRNQGAVVKVFGDSCDGVYKQIAESMGFDFIKIEEHVGKRGVLSRSMQYVDTPLVMFVDSDTVLPQGAVLDMLKYFDDHVGGVGTNITINKSGHPVSYSSEFVERVREVIFKSISKNSSVMVLDGRCAMYRADLVKPLLTSKEFTENIVMGRKSKLGDDRQITSYLIRQGYRTVKDYNVYVRTYQYSSFKKFFRQQVRWGRVGWTYFFKDIWNGTARKAGALYTFDLLYMYLLPLFTTIIAILSLIYILPHIHFFFGGSGKIVYADAYLRLRLHDPSFIPIFWIIRTIVGFLNTAAIVIFGVTISVTIKKERFRVFAYGALGLLLMYISTIYALFTFFKQDKWLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A40</accession>
    <name>Q97A40_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoglycolate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0993131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917200.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A40"/>
    <dbReference type="STRING" id="273116.gene:9381762"/>
    <dbReference type="PaxDb" id="273116-14325187"/>
    <dbReference type="GeneID" id="1442048"/>
    <dbReference type="KEGG" id="tvo:TVG0993131"/>
    <dbReference type="eggNOG" id="arCOG02293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_11_3_2"/>
    <dbReference type="OrthoDB" id="31229at2157"/>
    <dbReference type="PhylomeDB" id="Q97A40"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050155">
        <property type="entry name" value="HAD-like_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434:SF1">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="207" mass="23392" checksum="F4A4861B9643A470" modified="2001-10-01" version="1">MKIKGVIFDFDGTLLDSVDVRITSWQEAFKDFGVNVPRDVIKPMIGYPGVDLARKFVNNPLDVEMLQEDYFLKKIDSVKLFQDVIPTIHRLKDRGIKVSVVTSTRRSVMNRFDLPVDTVVTIDDVSKGKPDPEPYIIALKKMAVEPKECVVVGDIENDLVPGKKIMCTTVLVKHGRDVHSPFADYEIENISDLIKIIEEIESKSQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A42</accession>
    <name>Q97A42_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0992082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917198.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A42"/>
    <dbReference type="STRING" id="273116.gene:9381760"/>
    <dbReference type="PaxDb" id="273116-14325185"/>
    <dbReference type="GeneID" id="1442046"/>
    <dbReference type="KEGG" id="tvo:TVG0992082"/>
    <dbReference type="eggNOG" id="arCOG04373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137479_3_2_2"/>
    <dbReference type="OrthoDB" id="10763at2157"/>
    <dbReference type="PhylomeDB" id="Q97A42"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051614">
        <property type="entry name" value="UPF0045_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00106">
        <property type="entry name" value="MTH1187 family thiamine-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777:SF1">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="MTH1187/YkoF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-binding protein" evidence="2">
        <location>
            <begin position="4"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="96" mass="10770" checksum="E45226152706E149" modified="2001-10-01" version="1">MILAEITYIPIGEGTSASKYVNAALAEFKKMGIAFYPNSMGTVIEAKDLETIFEAVKRGEEAIIQKGIKRVETYLKIDHRIDAENSVQKKISELKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A51</accession>
    <name>TRPB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Tryptophan synthase beta chain</fullName>
            <ecNumber>4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trpB</name>
        <name type="ordered locus">TV0959</name>
        <name type="ORF">TVG0983765</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A51"/>
    <dbReference type="SMR" id="Q97A51"/>
    <dbReference type="STRING" id="273116.gene:9381751"/>
    <dbReference type="PaxDb" id="273116-14325176"/>
    <dbReference type="KEGG" id="tvo:TVG0983765"/>
    <dbReference type="eggNOG" id="arCOG01432">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042858_1_0_2"/>
    <dbReference type="OrthoDB" id="371827at2157"/>
    <dbReference type="PhylomeDB" id="Q97A51"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052684">
        <property type="term" value="F:L-serine hydro-lyase (adding indole, L-tryptophan-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006316">
        <property type="entry name" value="Trp_synth_b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009057">
        <property type="entry name" value="PRK12391.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01415">
        <property type="entry name" value="trpB_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF6">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500824">
        <property type="entry name" value="TrpB_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000099060" description="Tryptophan synthase beta chain">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="426" mass="47544" checksum="ABD99604E9BE6D26" modified="2001-10-01" version="1">MIRIDLKQDDMPDHWYNILPDLPEELPTPRDETGEAFETLKKAVPTKVLEYEFSGERYPKIPGEIYEKYMQVGRPTPIIRAKNLEEFLGGNIKIYLKMESYTYSGSHKINSALAHVFFAKQDNAKFVSTETGAGQWGSAVALASALFGVDSHIFMVRTSFYAKPYRKYMMYMYGAHPHPSPSEFTEYGKEVLKKNPDTPGSLGLAISEAIHYALDNGGKYIAGSVINSDILFKTIAGMEAKKQMEMAGEDPDYVVGVVGGGSNYAALAFPFLADELQSGKVKRTYIASGSKEVPKMTEGEYRYDYPDTGKVLPLLKMYTIGYDFIPPAVYAGGLRYHAVAPTLSLLMNKGIVQARDYDQEEAFKWARIFSEKEGYIPAPETSHALPILKEIADSNRGEREKKTVLVSFSGHGLLDLGNYAEAMHFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A73</accession>
    <name>Q97A73_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acylaminoacyl-peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0965471</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917170.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A73"/>
    <dbReference type="STRING" id="273116.gene:9381729"/>
    <dbReference type="ESTHER" id="thevo-TVG0965471">
        <property type="family name" value="ACPH_Peptidase_S9"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325154"/>
    <dbReference type="GeneID" id="1442018"/>
    <dbReference type="KEGG" id="tvo:TVG0965471"/>
    <dbReference type="eggNOG" id="arCOG01646">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460526_0_0_2"/>
    <dbReference type="OrthoDB" id="25019at2157"/>
    <dbReference type="PhylomeDB" id="Q97A73"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF27">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE FAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="393"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="592" mass="67733" checksum="B83C1F4BA90F0FCE" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-31" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A95</accession>
    <name>TRI_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Tricorn protease</fullName>
            <ecNumber>3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">tri</name>
        <name type="ordered locus">TV0915</name>
        <name type="ORF">TVG0940548</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Tricorn degrades oligopeptides in a sequential manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the tricorn proteolytic complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S41B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917153.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A95"/>
    <dbReference type="SMR" id="Q97A95"/>
    <dbReference type="STRING" id="273116.gene:9381707"/>
    <dbReference type="MEROPS" id="S41.005"/>
    <dbReference type="PaxDb" id="273116-14325132"/>
    <dbReference type="GeneID" id="1442001"/>
    <dbReference type="KEGG" id="tvo:TVG0940548"/>
    <dbReference type="eggNOG" id="arCOG03384">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005503_1_0_2"/>
    <dbReference type="OrthoDB" id="25019at2157"/>
    <dbReference type="PhylomeDB" id="Q97A95"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd10828">
        <property type="entry name" value="cpPDZ_Tricorn-protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07562">
        <property type="entry name" value="Peptidase_S41_TRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001823">
        <property type="entry name" value="Tricorn protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000221">
        <property type="entry name" value="Tricorn protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000053">
        <property type="entry name" value="Tricorn protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.44">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.60">
        <property type="entry name" value="Tricorn protease N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028204">
        <property type="entry name" value="Tricorn_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029414">
        <property type="entry name" value="Tricorn_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012393">
        <property type="entry name" value="Tricorn_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43253">
        <property type="entry name" value="TRICORN PROTEASE HOMOLOG 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43253:SF1">
        <property type="entry name" value="TRICORN PROTEASE HOMOLOG 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14685">
        <property type="entry name" value="PDZ_Tricorn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26550">
        <property type="entry name" value="Tricorn_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14684">
        <property type="entry name" value="Tricorn_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26549">
        <property type="entry name" value="Tricorn_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036421">
        <property type="entry name" value="Tricorn_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69304">
        <property type="entry name" value="Tricorn protease N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <feature type="chain" id="PRO_0000207198" description="Tricorn protease">
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            <begin position="1"/>
            <end position="1030"/>
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        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Binds the substrate's C-terminus" evidence="1">
        <location>
            <begin position="93"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Seven-bladed beta propeller" evidence="1">
        <location>
            <begin position="286"/>
            <end position="635"/>
        </location>
    </feature>
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        <location>
            <begin position="641"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="PDZ-like">
        <location>
            <begin position="721"/>
            <end position="816"/>
        </location>
    </feature>
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        <location>
            <begin position="817"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="706"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="926"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="984"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="877"/>
            <end position="879"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="2">
        <location>
            <begin position="954"/>
            <end position="956"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer; via amide nitrogen" evidence="2">
        <location>
            <position position="927"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P96086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1030" mass="116998" checksum="410BE79E1117DB9D" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AC1</accession>
    <name>Q97AC1_THEVO</name>
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            <fullName evidence="1">TVG0907198 protein</fullName>
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    <gene>
        <name evidence="1" type="ORF">TVG0907198</name>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmata</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AC1"/>
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    <dbReference type="KEGG" id="tvo:TVG0907198"/>
    <dbReference type="HOGENOM" id="CLU_2766326_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="69" mass="7676" checksum="0ABC4FB5262302B1" modified="2001-10-01" version="1">MHGTICGNVFPLHVSIQENAYNSGSVKKCYHAHVTSLELMVTLGNMDEVTKEASMETIDTISMARIQEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AC9</accession>
    <name>Q97AC9_THEVO</name>
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        <submittedName>
            <fullName evidence="1">TVG0898622 protein</fullName>
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    </protein>
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        <name evidence="1" type="ORF">TVG0898622</name>
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        <lineage>
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            <taxon>Thermoplasmata</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AC9"/>
    <dbReference type="PaxDb" id="273116-14325098"/>
    <dbReference type="GeneID" id="1441974"/>
    <dbReference type="KEGG" id="tvo:TVG0898622"/>
    <dbReference type="eggNOG" id="arCOG03473">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1335117_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34614">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34614:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="205" mass="24103" checksum="12A7C9F2EB047DA8" modified="2001-10-01" version="1">MHTISKVLHYFIRPAEAINLFDVVNTYVPRRQGLSLGLEFFLTTSHKLLDYKPTSANSTNWVKKTHLPLSMNFDPERITDNTQQYLMNKLYYEEKNTDHLYRISIQLYGNALKLFGKEDDTYFYDITPIYFEGKCCPLAFFGHNKDGKLNKLQINIAMIMNGKYGIPVVNKVFEGNINDVNTVYKMVYYPEVYCFSHFWIDQENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AD7</accession>
    <name>Q97AD7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0891084 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0891084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917117.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AD7"/>
    <dbReference type="STRING" id="273116.gene:9381665"/>
    <dbReference type="PaxDb" id="273116-14325090"/>
    <dbReference type="GeneID" id="1441965"/>
    <dbReference type="KEGG" id="tvo:TVG0891084"/>
    <dbReference type="eggNOG" id="arCOG05408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2613829_0_0_2"/>
    <dbReference type="OrthoDB" id="57000at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="78" mass="8824" checksum="B4B7F260FE1A0558" modified="2001-10-01" version="1">MERKCSNCGSEMESVGDMKFRVGGFTGIGGMFLGGWNDMMESTQTFSLYRCPQCGKVDFYEPRSDEGSEQNEKKHHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AG6</accession>
    <name>Q97AG6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0868615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2009-03" db="PDB data bank">
            <title>Crystal structure of rhodanese-related protein (TVG0868615) from Thermoplasma volcanium, Northeast Structural Genomics Consortium Target TvR109A.</title>
            <authorList>
                <person name="Forouhar F."/>
                <person name="Su M."/>
                <person name="Seetharaman J."/>
                <person name="Mao L."/>
                <person name="Xiao R."/>
                <person name="Foote E.L."/>
                <person name="Ciccosanti C."/>
                <person name="Belote R.L."/>
                <person name="Everett J.K."/>
                <person name="Nair R."/>
                <person name="Acton T.B."/>
                <person name="Rost B."/>
                <person name="Montelione G.T."/>
                <person name="Tong L."/>
                <person name="Hunt J.F."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 101-200</scope>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_052268467.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="3GK5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A=101-200"/>
    </dbReference>
    <dbReference type="PDBsum" id="3GK5"/>
    <dbReference type="AlphaFoldDB" id="Q97AG6"/>
    <dbReference type="SMR" id="Q97AG6"/>
    <dbReference type="STRING" id="273116.gene:9381636"/>
    <dbReference type="PaxDb" id="273116-14325061"/>
    <dbReference type="GeneID" id="32154025"/>
    <dbReference type="KEGG" id="tvo:TVG0868615"/>
    <dbReference type="eggNOG" id="arCOG02021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1406035_0_0_2"/>
    <dbReference type="OrthoDB" id="135517at2157"/>
    <dbReference type="EvolutionaryTrace" id="Q97AG6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050229">
        <property type="entry name" value="GlpE_sulfurtransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43031">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43031:SF17">
        <property type="entry name" value="SULFURTRANSFERASE YTWF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="114"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3GK5"/>
        </source>
    </evidence>
    <sequence length="208" mass="23854" checksum="3EDAC67F58ED9879" modified="2001-10-01" version="1">MKVLREDEESEIVKQFLDDNNSVIVDFDPIDLYEAVHPPFSIRLSIDRISAMPLSILRRRAAAFVSDPQTLSKLLNNDIAGKFDFIYDNSNRSWQKYYPFSYYRSINAADLYENIKAYTVLDVREPFELIFGSIANSINIPISELREKWKILERDKKYAVICAHGNRSAAAVEFLSQLGLNIVDVEGGIQSWIEEGYPVVYGRESAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AH1</accession>
    <name>Q97AH1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SIS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0861800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917083.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AH1"/>
    <dbReference type="SMR" id="Q97AH1"/>
    <dbReference type="STRING" id="273116.gene:9381631"/>
    <dbReference type="PaxDb" id="273116-14325056"/>
    <dbReference type="DNASU" id="1441931"/>
    <dbReference type="GeneID" id="1441931"/>
    <dbReference type="KEGG" id="tvo:TVG0861800"/>
    <dbReference type="eggNOG" id="arCOG00057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_2_0_2"/>
    <dbReference type="OrthoDB" id="372195at2157"/>
    <dbReference type="PhylomeDB" id="Q97AH1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="33"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="191"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="343" mass="38877" checksum="FF7BEA9D61870C5F" modified="2001-10-01" version="1">MTDILPEYRKKHPYVMYEMMKQIPEGIDKTLKAAQSVDYSFLGDDITFIGNGTAYHAGYIGSGIIEKYGLRRRVVQAYEYLNYLKYSSSFIGISHTGKTKSTVDAMLKARITGKTAGITHYRDSPLYNSVDVPILIEDEDKSLCNTKAFFNSAFAALYVSSIYLGKDIDFKSLRNEIAKHVNDDDKEMESASAELDNVKDIFVLGSGPNFPVAREAAQKIREATHLHAEGIELEEFNHGCTAVMDENTLVILVETERDKERANQIVRASSYVGSPTMAINGDAEISYYSDYQDEYLSPFVNMVPLYYLAYYLAVRRGINPDYLRFEDERYRNYDNTVFPPGAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AH5</accession>
    <name>Q97AH5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0853975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917079.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AH5"/>
    <dbReference type="SASBDB" id="Q97AH5"/>
    <dbReference type="STRING" id="273116.gene:9381627"/>
    <dbReference type="PaxDb" id="273116-14325052"/>
    <dbReference type="ABCD" id="Q97AH5">
        <property type="antibodies" value="23 sequenced antibodies"/>
    </dbReference>
    <dbReference type="GeneID" id="1441927"/>
    <dbReference type="KEGG" id="tvo:TVG0853975"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074283_0_0_2"/>
    <dbReference type="OrthoDB" id="56673at2157"/>
    <dbReference type="PhylomeDB" id="Q97AH5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF1">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="2">
        <location>
            <begin position="125"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="297" mass="32887" checksum="1EBCD37FB0255973" modified="2001-10-01" version="1">MDRAKGKSIAVYVGSMIVLIIVLLALLYVLSLFHIVPIKFSRILYAVVIGLVIYAIVRIVTKYVERFISLHSEFKHLKYIVFIISLLGYFIISLAVLAALGIDVSSVILGSAFLSAIIGLAAQSVLANVFGGLFLSIVRPFKLGDHIVVNTWQFPVSFPSYPPKYFSRDFIEGSIYRGIVSDISINYTSIALESGDVVKIPNNIMIQAAITLRHGSVTVQARYEVPKYINFNDIYNKIVDEVGTLPGARNIGVMIDETTVNTYIILVRGDFQGEDADLVRSQILQKVIGIVEPLKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AK7</accession>
    <name>Q97AK7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0804319 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0804319</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
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            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
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    </evidence>
    <sequence length="356" mass="40168" checksum="B2BD48FDDAE1685D" modified="2001-10-01" version="1">MGINQETISNLLDSLIFMEAYRKEKSDYDNFSSIIEARKKAADNFALEIKNTVTNFDSSPMGIDIADTKSKIIDFVNLAVSQLKEKIEERAKADQQLIKQKMEGDFNKAIGSLNLFMAQDPLTILDYTLYLSNEGGSYQARVLFKCDDGISYEFILNCGIIPELKESLEVSSIVKGVRLPVRKGRSLMSSEISVDYEKLDRYYLKYAEYSPKYLNVTFTDEDTMSEVSIFYPIDNPDMIKIDYKDDSGKISVDGDPVLSKNTDYKAIKDITKGIISVLQNVLSKKKSVSSILMDNQNIIEKGDITAFVKYIFQKYSYLIKDLISKGSMSIDQLKARLDPVNPSIMQDLVAIVGVSK</sequence>
</entry>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    <accession>Q97AY9</accession>
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            <fullName evidence="3">Medium-chain acyl-CoA ligase</fullName>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
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            <end position="374"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="518" mass="57780" checksum="1061517BD4E71D6F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B04</accession>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
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            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
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    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="AlphaFoldDB" id="Q97B04"/>
    <dbReference type="STRING" id="273116.gene:9381443"/>
    <dbReference type="PaxDb" id="273116-14324871"/>
    <dbReference type="GeneID" id="1441762"/>
    <dbReference type="KEGG" id="tvo:TVG0650773"/>
    <dbReference type="eggNOG" id="arCOG06971">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850744_0_0_2"/>
    <dbReference type="OrthoDB" id="55856at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="138" mass="15837" checksum="3C21DF12FE864FE3" modified="2001-10-01" version="1">MLNKDVKLTLRISEEEINEIDEFLNLNPEFGSRSEFIRHAVMQYISSKRISVVKDDLQKTHEGLILDENSKLIIKTAIENGFFKDENDLISAVLRESLEPFVLKFVKKKSKNMSDLMKALASFENFKQAGATARKDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B11</accession>
    <name>Q97B11_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0643747</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916907.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B11"/>
    <dbReference type="STRING" id="273116.gene:9381436"/>
    <dbReference type="PaxDb" id="273116-14324864"/>
    <dbReference type="GeneID" id="1441755"/>
    <dbReference type="KEGG" id="tvo:TVG0643747"/>
    <dbReference type="eggNOG" id="arCOG01282">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_2"/>
    <dbReference type="OrthoDB" id="25212at2157"/>
    <dbReference type="PhylomeDB" id="Q97B11"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004983">
        <property type="entry name" value="PRK06366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF107">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="6">
        <location>
            <begin position="264"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="388" mass="41574" checksum="6BB0339D34AC4E3D" modified="2001-10-01" version="1">MNDVYIVSAKRTAIGKFGRSFSKIPAPQLGGAAIKAVIEDSGIDKSAIQEVIMGNVIQAGVGQNPAGQAARAAGLADEITKYTVNVVCASGMLAVESAAREIKLGEREIVIAGGMENMSNAPFLLPSDLRWGPKHLLHKNYKIDDAMLVDGLTDAFYFEHMGVSAERTAKKYGITREMADEYSVQSYERAIRATNTGEFSKEITVFSNLSQDEGIRKTTMEDLAKLPPAFDKNGILTAGNSAQLSDGGSALLLASEKAINEYGLKPIAKITGYESASLAPLDFVEAPIPATRKLLERQGKSIDYYDIVEHNEAFSIASIIVRDQLHIDNERFNVNGGAVAIGHPIGNSGSRIIVTLINALKERHMKTGLATLCHGGGGAHTLTLELVD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-19" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B13</accession>
    <name>RS17E_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S17e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps17e</name>
        <name type="ordered locus">TV0646</name>
        <name type="ORF">TVG0642098</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59788" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59788.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916905.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B13"/>
    <dbReference type="SMR" id="Q97B13"/>
    <dbReference type="STRING" id="273116.gene:9381434"/>
    <dbReference type="PaxDb" id="273116-14324862"/>
    <dbReference type="GeneID" id="1441753"/>
    <dbReference type="KEGG" id="tvo:TVG0642098"/>
    <dbReference type="eggNOG" id="arCOG01885">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2115608_0_0_2"/>
    <dbReference type="OrthoDB" id="52479at2157"/>
    <dbReference type="PhylomeDB" id="Q97B13"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002242">
        <property type="entry name" value="PRK01151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000141569" description="Small ribosomal subunit protein eS17">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="75" mass="8826" checksum="7265DA77AAB3BF6F" modified="2001-12-19" version="2">MGSIRPFNVKRTAEEIAEKYPSLFTEKFEENKRKLEKMMPDVSKRTINIISGYLTRYVVKKKEKAEREIEENAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B21</accession>
    <name>Q97B21_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0630443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916896.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B21"/>
    <dbReference type="STRING" id="273116.gene:9381426"/>
    <dbReference type="PaxDb" id="273116-14324854"/>
    <dbReference type="GeneID" id="1441744"/>
    <dbReference type="KEGG" id="tvo:TVG0630443"/>
    <dbReference type="eggNOG" id="arCOG05369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893173_0_0_2"/>
    <dbReference type="OrthoDB" id="380233at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="304" mass="34622" checksum="D20FB8E440771E6F" modified="2001-10-01" version="1">MNPFLSAILSIVNAIVILYTATLSPLWYYIVYYGLNPKFSYSIILKPPGSFYTFYNFISINFYTETLTIASLIFIIAISAMMIIYGVNLLRRALLALLSISLYFVAVRAMLALVLLSYSIYLPFWHFGGINWFSFLNVNGFHMDQGSGNASGVLNFIFLSTYFWALLALLSMLVFREAAMLLLVIVIPLFVGLSFLDTFRKVAIRLISLFVELSFLPVYVLIDLYLAHVFSFDFILELGIIAISPAIPSILFAESVLLGFPLKILDPMKIYGLASLPLEKMAKNVDRLDWSEHMRSEFDYRRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B36</accession>
    <name>Q97B36_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Segregation and condensation protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0615712</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B36"/>
    <dbReference type="STRING" id="273116.gene:9381411"/>
    <dbReference type="PaxDb" id="273116-14324839"/>
    <dbReference type="KEGG" id="tvo:TVG0615712"/>
    <dbReference type="eggNOG" id="arCOG02613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045647_5_4_2"/>
    <dbReference type="PhylomeDB" id="Q97B36"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051304">
        <property type="term" value="P:chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005234">
        <property type="entry name" value="ScpB_csome_segregation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298:SF2">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04079">
        <property type="entry name" value="SMC_ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019345">
        <property type="entry name" value="ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19772" checksum="0499C0077451A1B1" modified="2001-10-01" version="1">MHIKYDQAMDDETKVEAILYATRNPLSVRSISLILGIEAGAISRIIKKLRLEYKKRNTSLEIAKIGNKYRIQLKKEYYDFAYRVMEPELSKYETGFLATVALNEGASLSFFRKRYGSRTDDMISKLKTMSLIRTSKKGNGTAIYLGENFEKVFGITKKELLERATEYDRQN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B53</accession>
    <name>SYFB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheT</name>
        <name type="ordered locus">TV0605</name>
        <name type="ORF">TVG0596849</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916863.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B53"/>
    <dbReference type="SMR" id="Q97B53"/>
    <dbReference type="STRING" id="273116.gene:9381393"/>
    <dbReference type="PaxDb" id="273116-14324820"/>
    <dbReference type="GeneID" id="1441711"/>
    <dbReference type="KEGG" id="tvo:TVG0596849"/>
    <dbReference type="eggNOG" id="arCOG00412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020279_3_0_2"/>
    <dbReference type="OrthoDB" id="10073at2157"/>
    <dbReference type="PhylomeDB" id="Q97B53"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00284">
        <property type="entry name" value="Phe_tRNA_synth_beta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022918">
        <property type="entry name" value="Phe_tRNA_ligase_beta2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000127015" description="Phenylalanine--tRNA ligase beta subunit">
        <location>
            <begin position="1"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="1">
        <location>
            <begin position="268"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="537" mass="61141" checksum="D60CC93E6011A9EC" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B54</accession>
    <name>Q97B54_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribbon-helix-helix protein CopG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0595213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916862.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B54"/>
    <dbReference type="STRING" id="273116.gene:9381392"/>
    <dbReference type="PaxDb" id="273116-14324819"/>
    <dbReference type="GeneID" id="1441710"/>
    <dbReference type="KEGG" id="tvo:TVG0595213"/>
    <dbReference type="eggNOG" id="arCOG06966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165893_0_0_2"/>
    <dbReference type="OrthoDB" id="25654at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="5"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="107" mass="12090" checksum="D03087F0BBEC8A61" modified="2001-10-01" version="1">MVEEKRITVRLPLSLVDEIQNLVDTGEFESMSDVVRRALVYLTESRTAKKEYVKVDLLIPKKIVSDSDGKIGTSITLDDLASLVLDRMNSRVARDAIKKIVNEENIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B81</accession>
    <name>Q97B81_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Indolepyruvate oxidoreductase subunit IorA</fullName>
            <shortName evidence="14">IOR</shortName>
            <ecNumber evidence="3 14">1.2.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Indolepyruvate ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">TVG0563624</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>indole-3-pyruvate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = (indol-3-yl)acetyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12645"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57271"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="1.2.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14 15">Binds 2 [4Fe-4S] clusters. In this family the first cluster has a non-standard and varying [4Fe-4S] binding motif CX(2)CX(2)CX(4-5)CP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 14">Heterodimer of the IorA and IorB subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="3 14"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916835.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B81"/>
    <dbReference type="STRING" id="273116.gene:9381362"/>
    <dbReference type="PaxDb" id="273116-14324791"/>
    <dbReference type="GeneID" id="1441093"/>
    <dbReference type="KEGG" id="tvo:TVG0563624"/>
    <dbReference type="eggNOG" id="arCOG01609">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017727_0_0_2"/>
    <dbReference type="OrthoDB" id="19071at2157"/>
    <dbReference type="PhylomeDB" id="Q97B81"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043805">
        <property type="term" value="F:indolepyruvate ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02008">
        <property type="entry name" value="TPP_IOR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000039">
        <property type="entry name" value="Indolepyruvate oxidoreductase subunit IorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045025">
        <property type="entry name" value="HACL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017721">
        <property type="entry name" value="IorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710:SF7">
        <property type="entry name" value="INDOLEPYRUVATE OXIDOREDUCTASE SUBUNIT IORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006439">
        <property type="entry name" value="Indolepyruvate_ferr_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 14 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="16">
        <location>
            <begin position="535"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="16">
        <location>
            <begin position="565"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="544"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="547"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="550"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="573"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="576"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="579"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="583"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR006439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR006439-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAB59718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="604" mass="65909" checksum="1A5E933D3B7180EB" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BA4</accession>
    <name>Q97BA4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0542920 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0542920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BA4"/>
    <dbReference type="STRING" id="273116.gene:9381338"/>
    <dbReference type="PaxDb" id="273116-14324768"/>
    <dbReference type="KEGG" id="tvo:TVG0542920"/>
    <dbReference type="eggNOG" id="arCOG00551">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1187827_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11714">
        <property type="entry name" value="GINS_A_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="210" mass="24674" checksum="0D16CDFA358AF0FF" modified="2001-10-01" version="1">MYAEQSHRKIMAIDQALYRRVREVIKELQVEADQQLKKQDIQEYMKTTRLIETIKSDFRSFFQRRYEKIVSKAIYDINSEIISVLTEEERGFIEKIHNLIMSEYDSFLPTLKDIAVEENQDQTTTTEVEKPMVISEPSNPTSQEKPIKDQAQTEKKEIEKKDVLVMITQDLPPIAQADRNYILHANDLVYLSEDIARILEKRNSAIILDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BA9</accession>
    <name>Q97BA9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0538221 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0538221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916806.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BA9"/>
    <dbReference type="STRING" id="273116.gene:9381333"/>
    <dbReference type="PaxDb" id="273116-14324763"/>
    <dbReference type="GeneID" id="1441064"/>
    <dbReference type="KEGG" id="tvo:TVG0538221"/>
    <dbReference type="eggNOG" id="arCOG05330">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194224_0_0_2"/>
    <dbReference type="OrthoDB" id="17596at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="62" mass="7433" checksum="0DA23BD0C16330E0" modified="2001-10-01" version="1">MDKICDVVECSNERFKTVPAELAKKVFSLKTDKTKVHLCKEHYKEYKKKTKEERDVDRADWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BB1</accession>
    <name>Q97BB1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="6">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0537100</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="6"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BB1"/>
    <dbReference type="STRING" id="273116.gene:9381331"/>
    <dbReference type="PaxDb" id="273116-14324761"/>
    <dbReference type="KEGG" id="tvo:TVG0537100"/>
    <dbReference type="eggNOG" id="arCOG00175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="PhylomeDB" id="Q97BB1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050093">
        <property type="entry name" value="ABC_SmlMolc_Importer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781">
        <property type="entry name" value="SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781:SF4">
        <property type="entry name" value="SPERMIDINE_PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="294" mass="33507" checksum="2F2F5EFAFE5B3D18" modified="2001-10-01" version="1">MKVQIGKFQLSIKELSLSGRHNFIMGENGSGKSTFLKTIAGLIDPISGRIDIDGNDITNMPPWKRHIAYIPQNLLLFPQYKVMDNIAISIKYGQGDPEIFKEVVKVMHLEDLLERNIYQLSGGQQQRVAVARAIVSMPRILLMDEPFSMQDERSRMSLISNLLDLIDRYEIDYIYVTHNSRDLELGFDTLSIMYNGTIIESVRSIDDLQTYEGNSLIDYRDIIKIDDKYYKVGISSVIPAQDGYSSRCVKEGDYYLCVVDIDGDQYFVRSSFDAHSVRFDLSKARELSIASSLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BB9</accession>
    <name>IF5A_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Translation initiation factor 5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Hypusine-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>eIF-5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">eif5a</name>
        <name type="ordered locus">TV0537</name>
        <name type="ORF">TVG0527241</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions by promoting the formation of the first peptide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916795.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BB9"/>
    <dbReference type="SMR" id="Q97BB9"/>
    <dbReference type="STRING" id="273116.gene:9381322"/>
    <dbReference type="PaxDb" id="273116-14324752"/>
    <dbReference type="GeneID" id="1441053"/>
    <dbReference type="KEGG" id="tvo:TVG0527241"/>
    <dbReference type="eggNOG" id="arCOG04277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102600_3_0_2"/>
    <dbReference type="OrthoDB" id="23689at2157"/>
    <dbReference type="PhylomeDB" id="Q97BB9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04467">
        <property type="entry name" value="S1_aIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000038">
        <property type="entry name" value="Translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00085">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022847">
        <property type="entry name" value="Transl_elong_IF5A_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003076">
        <property type="entry name" value="PRK03999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0385">Hypusine</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142507" description="Translation initiation factor 5A">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="modified residue" description="Hypusine" evidence="1">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="129" mass="14679" checksum="689D95CFC2C58941" modified="2001-10-01" version="1">MSWQEAEVRELKVGRYILIDDVPCKIVEITMSKPGKHGEAKGRIVAIGVFDNQKRSVVYPVKHKVKIPIIEKKNAQVLSVMNNEAQLMDSETFETFVLPLDPEIADKVKPGMEVPYWEAMGVRKIMLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BC4</accession>
    <name>Q97BC4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="8">SRP receptor</shortName>
            <ecNumber evidence="8">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ftsY</name>
        <name evidence="12" type="ORF">TVG0523258</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="8"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916790.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BC4"/>
    <dbReference type="STRING" id="273116.gene:9381316"/>
    <dbReference type="PaxDb" id="273116-14324747"/>
    <dbReference type="GeneID" id="1441048"/>
    <dbReference type="KEGG" id="tvo:TVG0523258"/>
    <dbReference type="eggNOG" id="arCOG01227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_1_2"/>
    <dbReference type="OrthoDB" id="372188at2157"/>
    <dbReference type="PhylomeDB" id="Q97BC4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17874">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000053">
        <property type="entry name" value="Signal recognition particle receptor FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Signal recognition particle SRP54 helical bundle" evidence="11">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="86"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="10">
        <location>
            <begin position="87"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="94"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="176"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="234"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB59674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="287" mass="31791" checksum="47E6A84CF6422BBC" modified="2001-10-01" version="1">MFEKFKKKLVEIFSRKKIDPDEAAEEIPLKLIEADVSVETAEYLGNLVRNKLKDRDFSGELNDVLYASIKEILAIPKPDILNVNKKPFVIMFLGINGTGKTTTIGKVAKYLKSKGKSVVLSASDTFRAGAIEQLSMIGKEIGVEVIKHEKGSDPSSVAFDAIQHAKARNIDYVLVDTAGRMNTNKNLMDEMKKIKRVSNPDITLLVLDAVAGQDSINQARTFHENVGFDAVIVTKLDTDARGGSILSIYHDLRKPVLFACVGQGLDDIMQFDADWYIEKLMPENSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BD8</accession>
    <name>Q97BD8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0507890 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0507890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053935.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BD8"/>
    <dbReference type="STRING" id="273116.gene:9381301"/>
    <dbReference type="PaxDb" id="273116-14324733"/>
    <dbReference type="GeneID" id="1441034"/>
    <dbReference type="KEGG" id="tvo:TVG0507890"/>
    <dbReference type="eggNOG" id="arCOG02089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_527495_0_0_2"/>
    <dbReference type="OrthoDB" id="56770at2157"/>
    <dbReference type="PhylomeDB" id="Q97BD8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902:SF3">
        <property type="entry name" value="NPCBM-ASSOCIATED, NEW3 DOMAIN OF ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902">
        <property type="entry name" value="S-LAYER DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="502"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="533" mass="58449" checksum="590731E2188E3096" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BH6</accession>
    <name>Q97BH6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF763 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0468151</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916738.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BH6"/>
    <dbReference type="STRING" id="273116.gene:9381261"/>
    <dbReference type="PaxDb" id="273116-14324694"/>
    <dbReference type="GeneID" id="1440996"/>
    <dbReference type="KEGG" id="tvo:TVG0468151"/>
    <dbReference type="eggNOG" id="arCOG04253">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061722_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BH6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008482">
        <property type="entry name" value="DUF763"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38597">
        <property type="entry name" value="BLL3834 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38597:SF1">
        <property type="entry name" value="BLL3834 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05559">
        <property type="entry name" value="DUF763"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="40464" checksum="2341CFC27983EDCE" modified="2001-10-01" version="1">MERKGTSILPLHYGHPPEYLYCRAVKLSGLLSELVIENYGVEELIRRIADPFWFHSMSLAIGFDWNSSGTTVFALSALKDYFSKRPGEIVICGGKGKKMGSMQDELESARALGFITASESNKLSVDAKRVAKIDNNLLQDGFDIYLQFLVVSSKGTHAVVQQGMNTQLRSARRYHWTLGNTFAGIDGRNGISSELVLDRTLDLSAKESEGNRKGIVDVIREKSFLNVPGEKQATLDGFVMPELRMDYRINWNKVRNLYEYNVDDFEQVYMAPGIGKSTIRALSFLAEIVYGEKPSFKDPVKYSFALGGKDGVPKPVNVYDYDKAIEFYQDVLKHIEIGDQSRKNLVKLLARMSAEASRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BJ5</accession>
    <name>Q97BJ5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin prenyltransferase UbiX</fullName>
            <ecNumber evidence="6">2.5.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ubiX</name>
        <name evidence="8" type="ORF">TVG0450298</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dimethylallyl phosphate + FMNH2 = prenylated FMNH2 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.129" evidence="6"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916719.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BJ5"/>
    <dbReference type="STRING" id="273116.gene:9381241"/>
    <dbReference type="PaxDb" id="273116-14324675"/>
    <dbReference type="GeneID" id="1440977"/>
    <dbReference type="KEGG" id="tvo:TVG0450298"/>
    <dbReference type="eggNOG" id="arCOG01703">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074522_2_1_2"/>
    <dbReference type="OrthoDB" id="9540at2157"/>
    <dbReference type="PhylomeDB" id="Q97BJ5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106141">
        <property type="term" value="F:flavin prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1950:FF:000001">
        <property type="entry name" value="Flavin prenyltransferase UbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01984">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004685">
        <property type="entry name" value="PRK06029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374">
        <property type="entry name" value="FLAVIN PRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374:SF1">
        <property type="entry name" value="FLAVIN PRENYLTRANSFERASE PAD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6" id="KW-0288">FMN</keyword>
    <keyword evidence="1 6" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavoprotein" evidence="7">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="84"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="182" mass="19910" checksum="BD3244E7952DCE2F" modified="2001-10-01" version="1">MRIVVGVSGASGIPYAVKLLENLGQNEVHLIISENAKKVMKYEMNQDPSYLSSLASYTYSDDDFTAPVSSGSFLFDAMVIVPCSISTLSKIAVGISDTLITRAAAVSIKERRRLIIVPREMPLSSIDLKNMLSLSENGVIIAPASPGFYNKPKSIDDLVSFVVSRILDLIGVKNELIKRWQD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BK7</accession>
    <name>RS28_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS28</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S28e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps28e</name>
        <name type="ordered locus">TV0448</name>
        <name type="ORF">TVG0437776</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916708.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BK7"/>
    <dbReference type="SMR" id="Q97BK7"/>
    <dbReference type="STRING" id="273116.gene:9381229"/>
    <dbReference type="PaxDb" id="273116-14324663"/>
    <dbReference type="GeneID" id="1440966"/>
    <dbReference type="KEGG" id="tvo:TVG0437776"/>
    <dbReference type="eggNOG" id="arCOG04314">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178987_2_1_2"/>
    <dbReference type="OrthoDB" id="7620at2157"/>
    <dbReference type="PhylomeDB" id="Q97BK7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000145">
        <property type="entry name" value="30S ribosomal protein S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_eS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003080">
        <property type="entry name" value="PRK04007.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000136864" description="Small ribosomal subunit protein eS28">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="69" mass="7488" checksum="118B83C560BA5DB9" modified="2001-10-01" version="1">MPNEATPAEVVEIIGRTGMTGEATTVKVRILSGRDQGRIIARNVLGPVRVGDILMLMETAREAKKLSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BL2</accession>
    <name>Q97BL2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0432809 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0432809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BL2"/>
    <dbReference type="STRING" id="273116.gene:9381222"/>
    <dbReference type="PaxDb" id="273116-14324658"/>
    <dbReference type="KEGG" id="tvo:TVG0432809"/>
    <dbReference type="eggNOG" id="arCOG00312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3003371_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="56" mass="6206" checksum="57872CCFC55346C7" modified="2001-10-01" version="1">MIKALQMVDKYKVETPASRPDNELIKDKSIIPPPVTIDDIPESMKIIQGLCVVANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BQ1</accession>
    <name>Q97BQ1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aspartate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0393535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916660.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BQ1"/>
    <dbReference type="STRING" id="273116.gene:9381182"/>
    <dbReference type="PaxDb" id="273116-14324619"/>
    <dbReference type="GeneID" id="1440918"/>
    <dbReference type="KEGG" id="tvo:TVG0393535"/>
    <dbReference type="eggNOG" id="arCOG00492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_6_2"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="PhylomeDB" id="Q97BQ1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000053">
        <property type="entry name" value="Aminotransferase, class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF19">
        <property type="entry name" value="KYNURENINE_ALPHA-AMINOADIPATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="45"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="402" mass="45684" checksum="1CE50D5AB95AB5DA" modified="2001-10-01" version="1">MNYKFSEIFRYIKPSEIRSLLKYTSDPELISFGGGMPNPESFPVEEMKEILADIMDNYGKKALQYGTTEGLDVLRDELAKYVLKTEGINAKREEIILTTGSQQALYAIAKIFVNPNDPVITEGPTYVGAISAFNANTANMMAIDIDENGMNVDELEAKIKELSQNGLKPKFIYVIPTFQNPAGTTMSLDRRKRLLEISERYEIPIVEDNPYGQLRYDGEPVQSIKSMDKNGNVIYLGTFSKVMAPGLRLGYVIAPREVIDKINLLKQGLDLASDSLSEYIAYEYLKRGAIYRQIPKTVDLYRKKRDLMIKSLEEYFPEAATWTKPHGGMFLWVSLDERIDTTKMLDRALKAKVAYVSGSAFYPHGEKHNSMRLNFTYSDDDKIVEGIKRLAYVINEEMEVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BR3</accession>
    <name>Q97BR3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF211 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0378908</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BR3"/>
    <dbReference type="STRING" id="273116.gene:9381169"/>
    <dbReference type="PaxDb" id="273116-14324607"/>
    <dbReference type="KEGG" id="tvo:TVG0378908"/>
    <dbReference type="eggNOG" id="arCOG04140">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179754_0_0_2"/>
    <dbReference type="OrthoDB" id="201945at2157"/>
    <dbReference type="PhylomeDB" id="Q97BR3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1340">
        <property type="entry name" value="MTH889-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003831">
        <property type="entry name" value="DUF211"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023129">
        <property type="entry name" value="MTH889-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240">
        <property type="entry name" value="DUF211 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240:SF1">
        <property type="entry name" value="DUF211 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02680">
        <property type="entry name" value="DUF211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160363">
        <property type="entry name" value="MTH889-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10535" checksum="3AA79575BF79BEF3" modified="2001-10-01" version="1">MNIRRIVLDVDKGLNRPTLVELASAIERVPGVEAVNIIVTEMDMETMGTNITVEGTNINYEELIESIENTGSAIHSVDEIAVGKRLIENVRRTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BR4</accession>
    <name>Q97BR4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transport system ATP-binding protein PA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0378375</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916645.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BR4"/>
    <dbReference type="STRING" id="273116.gene:9381168"/>
    <dbReference type="PaxDb" id="273116-14324606"/>
    <dbReference type="GeneID" id="1440903"/>
    <dbReference type="KEGG" id="tvo:TVG0378375"/>
    <dbReference type="eggNOG" id="arCOG00174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036171_2_0_2"/>
    <dbReference type="OrthoDB" id="50379at2157"/>
    <dbReference type="PhylomeDB" id="Q97BR4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42744">
        <property type="entry name" value="BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEMS INNER MEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42744:SF1">
        <property type="entry name" value="BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEMS INNER MEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="440"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="11"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="541" mass="60852" checksum="278FB5E836F9F4C3" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BT1</accession>
    <name>Q97BT1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec-independent protein translocase protein TatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tatC</name>
        <name evidence="6" type="ORF">TVG0363970</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with TatA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916628.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BT1"/>
    <dbReference type="STRING" id="273116.gene:9381151"/>
    <dbReference type="PaxDb" id="273116-14324589"/>
    <dbReference type="GeneID" id="1440886"/>
    <dbReference type="KEGG" id="tvo:TVG0363970"/>
    <dbReference type="eggNOG" id="arCOG01919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031942_3_2_2"/>
    <dbReference type="OrthoDB" id="15305at2157"/>
    <dbReference type="PhylomeDB" id="Q97BT1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009977">
        <property type="term" value="F:proton motive force dependent protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011532">
        <property type="entry name" value="TatC_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01912">
        <property type="entry name" value="TatC-Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF0">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="272" mass="30791" checksum="C8A4F8868734695C" modified="2001-10-01" version="1">MPNEILDLLQKNIDELRLRVTRALIGFFVFFLIFFTFRFEYINMLGRKIPMLYPDPYSNVGAQFLVLAESHVLPKKVELIAVKPTDAMVADIYSVMFLAILFSMPIIVTEIGRFVGPGLRKQEISAIRSLGIPAAVLFAIGSIFGFWFIFPDLFKIFFYFDSSVGAYPTMSISSFTSFLFVYIISFGLSFEMPVIMVALTRLKLVQANFWLRNWRYAVVGSLIFGLVFSPGVLGVSMMIMALPMMLLYVLGAFIAKRVEEKSEAMTATAPYN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-16" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BU5</accession>
    <name>RUXX_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Putative snRNP Sm-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0360</name>
        <name type="ORF">TVG0350334</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59502" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59502.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916614.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BU5"/>
    <dbReference type="SMR" id="Q97BU5"/>
    <dbReference type="STRING" id="273116.gene:9381137"/>
    <dbReference type="PaxDb" id="273116-14324575"/>
    <dbReference type="GeneID" id="1440872"/>
    <dbReference type="KEGG" id="tvo:TVG0350334"/>
    <dbReference type="eggNOG" id="arCOG00998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_11_1_2"/>
    <dbReference type="OrthoDB" id="371816at2157"/>
    <dbReference type="PhylomeDB" id="Q97BU5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01731">
        <property type="entry name" value="archaeal_Sm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00257">
        <property type="entry name" value="Lsm_RuxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022901">
        <property type="entry name" value="snRNP_Sm-like_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001963">
        <property type="entry name" value="PRK00737.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="chain" id="PRO_0000125601" description="Putative snRNP Sm-like protein">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="9"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="83" mass="9184" checksum="BF2ACB23C83CEDD5" modified="2002-04-16" version="2">MPKTVANTKPMDVLKNALSRNVLIDVKGNREYSGILEGYDVYMNVVLQNASEIINGENKGVFDRILVRGDNVIFVSPSKGDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BX9</accession>
    <name>Q97BX9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase M50 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0331095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BX9"/>
    <dbReference type="STRING" id="273116.gene:9381102"/>
    <dbReference type="TCDB" id="9.B.149.1.10">
        <property type="family name" value="the m50 peptidase (m50-p) family"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324541"/>
    <dbReference type="KEGG" id="tvo:TVG0331095"/>
    <dbReference type="eggNOG" id="arCOG04064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042134_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BX9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031293">
        <property type="term" value="P:membrane protein intracellular domain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06159">
        <property type="entry name" value="S2P-M50_PDZ_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001193">
        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325:SF3">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PROTEASE M50 MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE 2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01000">
        <property type="entry name" value="SREBPS2PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="503"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M50" evidence="6">
        <location>
            <begin position="132"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="525" mass="57779" checksum="A856E84037FB85B4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BY1</accession>
    <name>Q97BY1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alkaline phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0329157</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916579.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BY1"/>
    <dbReference type="STRING" id="273116.gene:9381100"/>
    <dbReference type="PaxDb" id="273116-14324539"/>
    <dbReference type="GeneID" id="1440837"/>
    <dbReference type="KEGG" id="tvo:TVG0329157"/>
    <dbReference type="eggNOG" id="arCOG03117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1021653_0_0_2"/>
    <dbReference type="OrthoDB" id="56381at2157"/>
    <dbReference type="PhylomeDB" id="Q97BY1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051311">
        <property type="entry name" value="DedA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709">
        <property type="entry name" value="ALKALINE PHOSPHATASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709:SF6">
        <property type="entry name" value="UNDECAPRENYL PHOSPHATE TRANSPORTER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="7">
        <location>
            <begin position="107"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="272" mass="30911" checksum="B4777A41945B5431" modified="2001-10-01" version="1">MPQKFGKNGFVQVRDKMRINFRSLFLKVNPAGSASMSKYYYYAFIFSILILFASAVSITNGYFHYVNVYSYYSYQHKILAPFSLTGYEGMFLVVAFAPLPDYLILPFYGYLSSVGEFNIFLTFLVSVAAMLFEMGIEYAGGRLAGRPLLLKVLSYFKITEKDLEVADHWIKDHGSFSIFIATFIPYFKNVTSLAAGTLKMNAGLFFLSNLVGFSIRFGLLLYIGYTGIFVLTPSWDYSHRLATALVGVLALMYIIAYTWRKISTILAYRVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BZ7</accession>
    <name>Q97BZ7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosome maturation protein SDO1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0318482</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916563.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BZ7"/>
    <dbReference type="STRING" id="273116.gene:9381083"/>
    <dbReference type="PaxDb" id="273116-14324523"/>
    <dbReference type="GeneID" id="1440821"/>
    <dbReference type="KEGG" id="tvo:TVG0318482"/>
    <dbReference type="eggNOG" id="arCOG04187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043216_2_0_2"/>
    <dbReference type="OrthoDB" id="84504at2157"/>
    <dbReference type="PhylomeDB" id="Q97BZ7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046928">
        <property type="entry name" value="SDO1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF4">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SDO1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20268">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS central" evidence="3">
        <location>
            <begin position="100"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS C-terminal" evidence="4">
        <location>
            <begin position="164"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25978" checksum="228D6D878AABE725" modified="2001-10-01" version="1">MVRVEDAIVARLESHGYKFEILVDPDAIERIRKGNIDIENDLAMPEVYKDARKGEKASEESLKEAFKTTDIAQIVVDIVKKGQIQLTTEQRREMYDERRKQIVNIIAREGINPQTNTPHTPYRISQAMEEAKVKIDPFKSAEEQVQGVLKAIMPIIPIRFEKAKLAVKLVGDAYGKLYGELAKSGYITKEEWGKDGSWMGILEVPAGIQGDIIESLSRRGGDKVQIRVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C19</accession>
    <name>Q97C19_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron III dicitrate transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0296872</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916541.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C19"/>
    <dbReference type="STRING" id="273116.gene:9381060"/>
    <dbReference type="PaxDb" id="273116-14324500"/>
    <dbReference type="GeneID" id="1440799"/>
    <dbReference type="KEGG" id="tvo:TVG0296872"/>
    <dbReference type="eggNOG" id="arCOG01007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_2_2"/>
    <dbReference type="PhylomeDB" id="Q97C19"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06550">
        <property type="entry name" value="TM_ABC_iron-siderophores_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3470.10:FF:000001">
        <property type="entry name" value="Vitamin B12 ABC transporter permease BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472:SF25">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MJ0876-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FERRIC ENTEROBACTIN TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="354" mass="38220" checksum="5347A99FFDB3D588" modified="2001-10-01" version="1">MVFSDARVMGSRIGKSLTGYLSIILLAFVSLISLGIGSVNVPLKNTAEIIWDQIPFLNALHYFSVPYTQYAIITQLREPQIVGGIIVGSTLAVGGTVVQSIFRNPITEPYIIGISSGAALGAVMTIALSLNFLGYYTLETNAFVFSIITVVIVYLLSFRHGRAPPTFLLLIGISLSFFASSIVSFLIFTNVNLAGSEYFWLLGSLQTLNWQEISMLIPISLLTSIAIFFMRNELDALQMGDEHAHSIGVNVERSKAMLIALVTLSTSAVVSVSGLIGFVGLIMPHISRIIFGGSNKYVIPSSALFGAIFLILANDLTKVVVHGEVVPIGIITGIIGVPFFIYLLSRMAKGSYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C27</accession>
    <name>Q97C27_THEVO</name>
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        <submittedName>
            <fullName evidence="1">TVG0291516 protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">TVG0291516</name>
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    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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    <dbReference type="GeneID" id="42990933"/>
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    <dbReference type="OrthoDB" id="386111at2157"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="61" mass="6767" checksum="6D187E411FCFEF52" modified="2001-10-01" version="1">MLKSYLILAYATMPVHSGMGRAPTSYEAIRIKVVNRKPMNHTEGTLVLHGGEEVRSYCSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C33</accession>
    <name>Q97C33_THEVO</name>
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        <submittedName>
            <fullName evidence="1">TVG0282820 protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">TVG0282820</name>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C33"/>
    <dbReference type="STRING" id="273116.gene:9381045"/>
    <dbReference type="PaxDb" id="273116-14324486"/>
    <dbReference type="KEGG" id="tvo:TVG0282820"/>
    <dbReference type="eggNOG" id="arCOG05358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2299440_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="103" mass="12043" checksum="791426D02B2E9080" modified="2001-10-01" version="1">MLMVLSRKKDINSIVAERRRELRDLPTVLVPVWQIKIRERFGISVDREIAKYIVLAAHENGTWKRQRAMKKIEKLLQERGESKENSARIAKEIIDLVIGSAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C40</accession>
    <name>Q97C40_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyrosine recombinase XerA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">xerA</name>
        <name evidence="9" type="ORF">TVG0276761</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 'phage' integrase family. XerA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916521.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C40"/>
    <dbReference type="STRING" id="273116.gene:9381038"/>
    <dbReference type="PaxDb" id="273116-14324479"/>
    <dbReference type="GeneID" id="1440779"/>
    <dbReference type="KEGG" id="tvo:TVG0276761"/>
    <dbReference type="eggNOG" id="arCOG01241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_5_2"/>
    <dbReference type="OrthoDB" id="142231at2157"/>
    <dbReference type="PhylomeDB" id="Q97C40"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009037">
        <property type="term" value="F:tyrosine-based site-specific recombinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02055">
        <property type="entry name" value="Recomb_XerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033686">
        <property type="entry name" value="XerA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040815">
        <property type="entry name" value="recomb_XerA_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF41">
        <property type="entry name" value="INTEGRASE_RECOMBINASE PROTEIN MJ0367-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13495">
        <property type="entry name" value="Phage_int_SAM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0229">DNA integration</keyword>
    <keyword evidence="4 5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="8">
        <location>
            <begin position="3"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="7">
        <location>
            <begin position="100"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="5">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="283" mass="32474" checksum="0B3C5844958BB40E" modified="2001-10-01" version="1">MPAGSESYVENFVSYMVGERKSKYTIKEYKFLVSKFLAFVGKTPAEVTPMDIERYKTFLASKAHYSKASQYLAIKAIKLFYKSLDLRYPVNLTPPRRSMHMPVYLNEEEASRLLSASKKSVKYSAVISVLAYTGIRVGELCNLRIGDVDMQEGIIYVKSGKGDKDRIVVMPNECVSALKEYLNWRISVETPHDFLFISNKKSRYDPSTVERMVKRLSQEAGINKRVTPHVLRHTFATSVLRNGGDIRFIQQILGHSSVATTQIYTHLDDNALKDMYFKHRPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C42</accession>
    <name>Q97C42_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0275213 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0275213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C42"/>
    <dbReference type="PaxDb" id="273116-14324477"/>
    <dbReference type="KEGG" id="tvo:TVG0275213"/>
    <dbReference type="HOGENOM" id="CLU_3039268_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="54" mass="6273" checksum="C145474BB1DE694B" modified="2001-10-01" version="1">MLSYYYSKISNMAVSLVKAYRKEYYGLNIDRDVNAYTNVARSVRTCSKRAVKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C44</accession>
    <name>Q97C44_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hypoxanthine-guanine phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0273888</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C44"/>
    <dbReference type="STRING" id="273116.gene:9381034"/>
    <dbReference type="PaxDb" id="273116-14324475"/>
    <dbReference type="KEGG" id="tvo:TVG0273888"/>
    <dbReference type="eggNOG" id="arCOG00040">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080904_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97C44"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="21"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="224" mass="26025" checksum="1924C05CABEB13ED" modified="2001-10-01" version="1">MIPDYDYMANKFKATLVTWEDIDRWCGKISRDVADTFKVDTIIGIARGGVVPARILSDRLWVKDMLSVKTEHWGLTATKDGEAVLKTKIDTDLRGRKVLVVDDITDTGESMSVAYNYIKSLHPDELKSATMLHINRSKYVPDYYAELITEKNWAWFIFPWNVYEDLNNLIRKSISDYADIDKVRESLKQDYDLDTSNLDIETILEDLVHFGKMKRQNNRYVTVQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-23" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C45</accession>
    <name>ARCC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Carbamate kinase</fullName>
            <ecNumber>2.7.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">arcC</name>
        <name type="ordered locus">TV0260</name>
        <name type="ORF">TVG0273901</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>hydrogencarbonate + NH4(+) + ATP = carbamoyl phosphate + ADP + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Metabolic intermediate metabolism; carbamoyl phosphate degradation; CO(2) and NH(3) from carbamoyl phosphate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbamate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916517.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C45"/>
    <dbReference type="SMR" id="Q97C45"/>
    <dbReference type="STRING" id="273116.gene:9381033"/>
    <dbReference type="PaxDb" id="273116-14324474"/>
    <dbReference type="GeneID" id="1440775"/>
    <dbReference type="KEGG" id="tvo:TVG0273901"/>
    <dbReference type="eggNOG" id="arCOG00863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076278_0_0_2"/>
    <dbReference type="OrthoDB" id="31128at2157"/>
    <dbReference type="PhylomeDB" id="Q97C45"/>
    <dbReference type="UniPathway" id="UPA00996">
        <property type="reaction ID" value="UER00366"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008804">
        <property type="term" value="F:carbamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019546">
        <property type="term" value="P:L-arginine deiminase pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04235">
        <property type="entry name" value="AAK_CK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000007">
        <property type="entry name" value="Carbamate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003964">
        <property type="entry name" value="Carb_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009007">
        <property type="entry name" value="PRK12352.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30409">
        <property type="entry name" value="CARBAMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30409:SF1">
        <property type="entry name" value="CARBAMATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000723">
        <property type="entry name" value="Carbamate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01469">
        <property type="entry name" value="CARBMTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0056">Arginine metabolism</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000185144" description="Carbamate kinase">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="305" mass="33464" checksum="CC9E8F342E8E0683" modified="2001-10-01" version="1">MVRIVIALGGNALLRNGEPRNYEVQYSHAIETFKIIRDITEKNETVITHGNGPQVGDIQQSHEISGIMANLHQSVAMSQGYIGEILANAYDEVRSKYSLSKSIFTIITRSVVNRNDPAFASPEKPIGRYYSDQEVDEAKRNGWVMKKFKDGWRRVVPSPEPIEILEEKAIEELVREGHIPLAVGGGGIPVVKENGRIVGIDAVIDKDIASSLLAAQLKADYFMILTDVESVYVNFGKPDQKPLGRIHLNEIEKYYAEGQFADGSMKPKVRAAINFVKNGGKAAFITNLENGSAALSGKAGTVIVP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-21" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C75</accession>
    <name>MRE11_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA double-strand break repair protein Mre11</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mre11</name>
        <name type="ordered locus">TV0229</name>
        <name type="ORF">TVG0238008</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Nuclease activity is regulated by Rad50.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760296.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C75"/>
    <dbReference type="SMR" id="Q97C75"/>
    <dbReference type="STRING" id="273116.gene:9381000"/>
    <dbReference type="PaxDb" id="273116-14324443"/>
    <dbReference type="DNASU" id="1440744"/>
    <dbReference type="GeneID" id="1440744"/>
    <dbReference type="KEGG" id="tvo:TVG0238008"/>
    <dbReference type="eggNOG" id="arCOG00397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026621_5_2_2"/>
    <dbReference type="PhylomeDB" id="Q97C75"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02044">
        <property type="entry name" value="Mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050535">
        <property type="entry name" value="DNA_Repair-Maintenance_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032885">
        <property type="entry name" value="Mre11_archaea-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337">
        <property type="entry name" value="COMPONENT OF ATP-DEPENDENT DSDNA EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF0">
        <property type="entry name" value="NUCLEASE SBCCD SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138702" description="DNA double-strand break repair protein Mre11">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02044"/>
        </source>
    </evidence>
    <sequence length="374" mass="42598" checksum="5AFE7C2084D9143E" modified="2001-10-01" version="1">MVRFLHMSDTHIGAKSLTIEEREQDYYDTFQEAVEIAIDEKVDFIIHSGDLFDTWIPGNRSMKVFRDAMMKLNDRQIPVFYVFGDHDRPRRNSESAAGIFDFLGLHILGRDEFAGIEREFSGMKVFIGGISNMKGYLRNQLKEEYKKAESASTGYKNSILISHQALDPIFIPEQCEAKVNDLPMNYSYIAMGHLHDFVEREIGPLLSYPGSTELKSDREINGLLKMGKGINVIDLDNGVASLHRVKLKKVRYQFKVESDPENYLEEIKSILDKYANAIGEKKPIVNLIINGQISMEEVYEDLKKINGAIFNRPQILKIVDMPNVPSGSSELREYFQAFLKDQKLTDLAMKVFQHTADGDKEAIIREAAGEAIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C79</accession>
    <name>Q97C79_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0232268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916479.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C79"/>
    <dbReference type="STRING" id="273116.gene:9380995"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324438"/>
    <dbReference type="DNASU" id="1440739"/>
    <dbReference type="GeneID" id="1440739"/>
    <dbReference type="KEGG" id="tvo:TVG0232268"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_7_3_2"/>
    <dbReference type="OrthoDB" id="11098at2157"/>
    <dbReference type="PhylomeDB" id="Q97C79"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04179">
        <property type="entry name" value="DPM_DPG-synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050256">
        <property type="entry name" value="Glycosyltransferase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090:SF7">
        <property type="entry name" value="RFBJ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE 4-DEOXY-4-FORMAMIDO-L-ARABINOSE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="226" mass="25668" checksum="13F140A5175A348A" modified="2001-10-01" version="1">MSIIEDNKISFVIPTINEEKTIGSVIDGIHENFPNAEIILVDTNSTDKTKEIAKSKGATVIEEPRKGYGRAYQTGIPAAKGDIIVCMDGDGTYPTDIVKPLIEILILDKVDFISCDRITFRTSTNYTTLHYVGNMVLNLTIRILFKVRMVDSQSGMWIFRSELYKKMKNLGYGMSFSQNIKIEAVRHGVFIEIPIRYGVRITKPKLKTWHDGFHNLFALFIKRVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CA0</accession>
    <name>Q97CA0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0209075 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0209075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CA0"/>
    <dbReference type="STRING" id="273116.gene:9380973"/>
    <dbReference type="PaxDb" id="273116-14324417"/>
    <dbReference type="KEGG" id="tvo:TVG0209075"/>
    <dbReference type="eggNOG" id="arCOG07856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140342_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="112" mass="13167" checksum="EE27A23D85A3F17A" modified="2001-10-01" version="1">MFVDSLYDASEIYDYVLENTHVIPIIDASERRGIVTEKLPDNSRIDINLRRKYPSLYSLKFGIERMFTILEISYKLSIWDVRNRSYNTAVGLKIIVYNLIIMSNMKLGENIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-30" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CA6</accession>
    <name>KPTA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable RNA 2'-phosphotransferase</fullName>
            <ecNumber evidence="1">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kptA</name>
        <name type="ordered locus">TV0196</name>
        <name type="ORF">TVG0201215</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes the 2'-phosphate from RNA via an intermediate in which the phosphate is ADP-ribosylated by NAD followed by a presumed transesterification to release the RNA and generate ADP-ribose 1''-2''-cyclic phosphate (APPR&gt;P). May function as an ADP-ribosylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916452.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CA6"/>
    <dbReference type="SMR" id="Q97CA6"/>
    <dbReference type="STRING" id="273116.gene:9380966"/>
    <dbReference type="PaxDb" id="273116-14324410"/>
    <dbReference type="GeneID" id="1441682"/>
    <dbReference type="KEGG" id="tvo:TVG0201215"/>
    <dbReference type="eggNOG" id="arCOG04063">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052998_4_1_2"/>
    <dbReference type="OrthoDB" id="24376at2157"/>
    <dbReference type="PhylomeDB" id="Q97CA6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000215">
        <property type="term" value="F:tRNA 2'-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.970">
        <property type="entry name" value="RNA 2'-phosphotransferase, Tpt1/KptA family, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00299">
        <property type="entry name" value="KptA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002745">
        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042081">
        <property type="entry name" value="RNA_2'-PTrans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022928">
        <property type="entry name" value="RNA_2'-PTrans_KptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042080">
        <property type="entry name" value="RNA_2'-PTrans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684">
        <property type="entry name" value="PUTATIVE PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684:SF2">
        <property type="entry name" value="TRNA 2'-PHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01885">
        <property type="entry name" value="PTS_2-RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000157496" description="Probable RNA 2'-phosphotransferase">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00299"/>
        </source>
    </evidence>
    <sequence length="235" mass="26797" checksum="842077175CE75244" modified="2001-10-01" version="1">MPELRYCHGPYRGKECPVCGKPGKIMMNEAEINEVSRTLAAVLRHDPGRYGIRLDSHGYARISSLVSMFRKRKGMRWMTDDHLVYLAETDPRKRYQISGVLIRAVYGHTIDVDLTDLPTDGIPDTLYYQSSTAEAPLVKEAGIYPSDKSWIHLSGTYRKSFVSGLYHIDDPLVLAVNARSMIENGIDIFRSNDDIYLTKQVPPEYITIAEKEEVVLTDEEKDDIKRVREKNSGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CC5</accession>
    <name>Q97CC5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0184113</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916432.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CC5"/>
    <dbReference type="STRING" id="273116.gene:9380947"/>
    <dbReference type="PaxDb" id="273116-14324391"/>
    <dbReference type="GeneID" id="1441662"/>
    <dbReference type="KEGG" id="tvo:TVG0184113"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_1_2"/>
    <dbReference type="OrthoDB" id="55972at2157"/>
    <dbReference type="PhylomeDB" id="Q97CC5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17319">
        <property type="entry name" value="MFS_ExuT_GudP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF399">
        <property type="entry name" value="FI19708P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="358"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="23"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="470" mass="52078" checksum="EF98170C87057FBA" modified="2001-10-01" version="1">MQETKNIVIHERSERRTRVRWAIVSLLFIAILFNYADREIWIVSEPAFAYSFGWSYSPTHLDALAIHNISLILFFWSLSYAIFNFPGGWIVDKLGLRKSMTTMFAIWSIFTALTAATFNFITMAIVRIVMGAGEGPVWPINSKVVKGWASRFDESKSFTLAGAGQAVGPVIGLIAGGILVTLFGWPSAFIFFGVLGLIFVAIWFFYVRDRPSEDSKVNENELAYIIEGKTNEEKEEKILPSKVNWKISARMIFGTQAGLGTLLVFLSFGYVLFTFLYWLPPLMFSTFAHTVEKSGLYSAAIDVALIAGFLGSGPFNDGLLKRFPDNKPLARRLGAIIPMVLMIVMVGLSYFTGKAHNLVGTAIFLGVGSGLMNLTVGSWAVNAVDLAPAGTSATVYGIYNGSLNFIGAFNAIIEGILFIRYGPVLAFTSSILFMLMFLGGYLGLIRKNTWERAMKYKEELSREAYSYLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CE4</accession>
    <name>Q97CE4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xylose isomerase-like TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0169833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916413.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CE4"/>
    <dbReference type="STRING" id="273116.gene:9380928"/>
    <dbReference type="PaxDb" id="273116-14324372"/>
    <dbReference type="GeneID" id="1441643"/>
    <dbReference type="KEGG" id="tvo:TVG0169833"/>
    <dbReference type="eggNOG" id="arCOG01894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068832_0_0_2"/>
    <dbReference type="OrthoDB" id="33250at2157"/>
    <dbReference type="PhylomeDB" id="Q97CE4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="103"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="341" mass="38858" checksum="D180D7AA274D6C69" modified="2001-10-01" version="1">MIRFGIAGIPLTSKGRTFIDSVEETHNLGLNALEVQLLRVNVQENSAEEFAGLRPRDVEDSIIIDVMRQDEEGNYVSVGINTEIEEDDIVRELFWNMARNYDDLETGGQLARELDITLSMHAPYYMDMIAGNDVADKSINHLRWALILGKYMGAKRVITHSGFYHGQKKDAVKKFAEVYADLVNEFPPDAGYPYIGVETSGKQEVFGTVKEVTDLAKKVNIEPILNFAHVHAITGGSLMEIKDFESIINEFKKYAKYDLYTEFSGVEFEGNEEKRLTAIKHGTLKFETLSEYLIDYPDDMTIISSSPLLEHDAQYMQIIYIRSYFKKMQRRHLAEKPVSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CE7</accession>
    <name>Q97CE7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dihydropteroate synthase</fullName>
            <ecNumber evidence="4">2.5.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0162982</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916409.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CE7"/>
    <dbReference type="SMR" id="Q97CE7"/>
    <dbReference type="STRING" id="273116.gene:9380924"/>
    <dbReference type="PaxDb" id="273116-14324368"/>
    <dbReference type="GeneID" id="1441639"/>
    <dbReference type="KEGG" id="tvo:TVG0162982"/>
    <dbReference type="eggNOG" id="arCOG01978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008023_0_2_2"/>
    <dbReference type="OrthoDB" id="371861at2157"/>
    <dbReference type="PhylomeDB" id="Q97CE7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="9">
        <location>
            <begin position="85"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="340" mass="38174" checksum="67D753EAF1EBEE66" modified="2001-10-01" version="1">MDGLIIPRRGAYKQEDGKYLFEVISEREPPVEHQTILENGIKKYIVKLDQSALKDLMRYYSVFPSYEDQAAERIFAGLDEKPIKPKIMGIINATPDSFYSGSRVLGNEDLIFKIIDEKPDIVDVGAESTRPGSKPVDPTKEYERLLPVIKIIRDYSDIPVSIDSRHPSVIKNLVKYEINYINDISGFTNKEMIDLAKDSSLYCVVMHMRGSPQDMQNFTNYDDVIVDVIGFLLDRANNLLANDVAFGRIISDPGIGFAKDLKGNIEILRNIKSFHIGMPLLVGTSRKTFIGTITGQAVEGRLPGTIGTSIYLANNRVSIIRVHDVRENKDAINTYLSLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CF2</accession>
    <name>Q97CF2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0154235</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916404.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="STRING" id="273116.gene:9380919"/>
    <dbReference type="PaxDb" id="273116-14324363"/>
    <dbReference type="GeneID" id="1441634"/>
    <dbReference type="KEGG" id="tvo:TVG0154235"/>
    <dbReference type="eggNOG" id="arCOG03669">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_258125_0_0_2"/>
    <dbReference type="OrthoDB" id="36243at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007981">
        <property type="entry name" value="Peptidase_A5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05317">
        <property type="entry name" value="Thermopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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</entry>
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            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="197" mass="22617" checksum="43E44FCE005E55CE" modified="2001-10-01" version="1">MILSEYFKEHPIKQRIIEGLYRSGISVKEGRFFANDVEISISEIAKTFGVNRKTVYDTVKLVEGNSHLKRVMESIMPMADVSNVALLTGNQIITVYTTLGHYPTVWKDVFMAISKYGCYIREIFSRNMSQDESFIRIIFYRPIPQKIIDQIKAIDGARNVEVKVSSDVDEILCRTCDIKICPTKYISDVEESKEDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CR5</accession>
    <name>Q97CR5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hypothetical membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0042148</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916293.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CR5"/>
    <dbReference type="STRING" id="273116.gene:9380801"/>
    <dbReference type="PaxDb" id="273116-14324250"/>
    <dbReference type="GeneID" id="1441522"/>
    <dbReference type="KEGG" id="tvo:TVG0042148"/>
    <dbReference type="eggNOG" id="arCOG01225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037460_3_0_2"/>
    <dbReference type="OrthoDB" id="31092at2157"/>
    <dbReference type="PhylomeDB" id="Q97CR5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010341">
        <property type="entry name" value="PRK13768.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="257" mass="29220" checksum="47B75D6FE3AF3CE1" modified="2001-10-01" version="1">MIGTLYVTGPAGTGKSTFSGALKEWLQRMEFDAAIINLDPGADYLPYEPDFDIREYISLEGIMSDYNLGPNGSQIVAADMIINFTDKIKEFTDELQDYYLVVDTPGQIELFTFRTSSTEIVDRISGEKSMIAYIADAPLATYPSGFIAQKMLYASVFSRFFKPMMFVLNKIDLVSDEDVETVKKWERDPDLLNEAFIDEKGNIEKDYFLNLLEAFKESNIMTKVYPVSSRDSFGFEDIYSNMSMFFTGGEDNDTYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CS7</accession>
    <name>Q97CS7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">4-aminobutyrate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0025258</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916281.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CS7"/>
    <dbReference type="STRING" id="273116.gene:9380789"/>
    <dbReference type="PaxDb" id="273116-14324238"/>
    <dbReference type="GeneID" id="1441510"/>
    <dbReference type="KEGG" id="tvo:TVG0025258"/>
    <dbReference type="eggNOG" id="arCOG00915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_0_2"/>
    <dbReference type="OrthoDB" id="6534at2157"/>
    <dbReference type="PhylomeDB" id="Q97CS7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004426">
        <property type="entry name" value="PRK05769.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF58">
        <property type="entry name" value="LEUCINE_METHIONINE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="449" mass="49740" checksum="523197F7F354A038" modified="2001-10-01" version="1">MMQEELNGIKIKVTPPGPEAKKIIELNDKYLARSTQSLPVVGKLGKGVYVEDVDGNVYLDFASGISVTNLGHLDPYVTQKVEEQLHKLWHFPGTDFYTEMQVLAAKSLIEVTPGKFNKKVFFTNSGTESVEAAIKIAKSYTHRERFIGFIGAFHGRTQGSLSFTASKPIHHKGFFPSMPGVEHVPFPNPYRNPFNIDGYEEPDELVNRVIDYIETYLLKTYVPPEDVAGILAEPIQGEGGYIVPPMNFFKELRKLADKYHIPLLIDEVQSGFGRTGKFFASEHFGVEPDVITLAKSIASGIPMGAAVMKEEMNFSENGLHSNTFGGNLIASAACVATIDEMKKLNAVENAAKQGAYMKKRLVELQNKYNEIGDVRGLGLMLAIDFVKDRRTKEPNSKLRNDVINNAFKNGLILLSTGSSAIRIIPPLIITQEQVDEGIEVLDKAIKMSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CT5</accession>
    <name>Q97CT5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Phosphoribosylaminoimidazole carboxamide formyl transferase/IMP cyclo-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0015470</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PurH family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916273.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CT5"/>
    <dbReference type="STRING" id="273116.gene:9380781"/>
    <dbReference type="PaxDb" id="273116-14324230"/>
    <dbReference type="GeneID" id="1441501"/>
    <dbReference type="KEGG" id="tvo:TVG0015470"/>
    <dbReference type="eggNOG" id="arCOG02824">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016316_5_2_2"/>
    <dbReference type="OrthoDB" id="52603at2157"/>
    <dbReference type="PhylomeDB" id="Q97CT5"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01421">
        <property type="entry name" value="IMPCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.20:FF:000001">
        <property type="entry name" value="Bifunctional purine biosynthesis protein PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002049">
        <property type="entry name" value="PRK00881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="489" mass="54378" checksum="53632F6CF2570ACE" modified="2001-10-01" version="1">MNFLISVYNKEGLTDFLARVKANIDYIYATGGTAKFLEKNGYTVRDTSEITGFSDLLGGRVKTLHPKIYAALLYREKNEASDLHFDVLVSNLYPFTGNTGNENEMIENIDIGGVSLTRAAAKNYKNVLVLSSPDDYTEAAEIISSGKNDAEYRKRMALKAFARMAEYDAIIYNGLSDLFKIEKENFFVVGKKGERLRYGENPDQEGFLYSDGSAYGVANAEKLNGKELSYNNILDADSAFDTALEFDEPTVVVMKHNTPSGVSSHNDIVNAFKRAWDADSESAYGSVIAVNRKVPLDLAKEMRHLFIEVLIAPEYDEYALSELRKKKNLRILRIPWIRDQSMRYRSISNGLLVQTPMASSFKELKLVTEKGADSKTIDDLLFAWKVVAHSRSNAIVFAKDKTTTGIGAGQTSRVESVRIAMERSGSRADGSVMASDAFFPFPDSIDVAAKAGIKAIIQPGGSIRDEEVIKKCNEYGIPMYFTGKRVFLH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-03" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CU0</accession>
    <name>IF6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 6</fullName>
            <shortName evidence="1">aIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif6</name>
        <name type="ordered locus">TV0011</name>
        <name type="ORF">TVG0009629</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 50S ribosomal subunit and prevents its association with the 30S ribosomal subunit to form the 70S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CU0"/>
    <dbReference type="SMR" id="Q97CU0"/>
    <dbReference type="STRING" id="273116.gene:9380776"/>
    <dbReference type="PaxDb" id="273116-14324225"/>
    <dbReference type="KEGG" id="tvo:TVG0009629"/>
    <dbReference type="eggNOG" id="arCOG04176">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071894_1_0_2"/>
    <dbReference type="OrthoDB" id="33582at2157"/>
    <dbReference type="PhylomeDB" id="Q97CU0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003131">
        <property type="entry name" value="PRK04046.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000153762" description="Translation initiation factor 6">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00032"/>
        </source>
    </evidence>
    <sequence length="218" mass="23572" checksum="E763F4A84BC18B2C" modified="2001-10-01" version="1">MVVIRKTSVMRSSFIGIYAKAWDDVAFITKMADEKTVNEFREVLQVDIKRISIDGSTLIGTMIAVNSNGIVLPYGSNIDGLDGIDGRNVLYLKDKINAVGNDIIANDHGAIIHKAFSNKARKEIEDTLGVETIRTSIGNILTVGSASVLTSKGMLVNPETDDDELEFLKDFFKVSVKSGTANFGSIYVGASIVANSKGILVGRDTTPIEIDRIDDVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q977Y0</accession>
    <name>Q977Y0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Isovaleryl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1333406</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917527.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q977Y0"/>
    <dbReference type="STRING" id="273116.gene:9382099"/>
    <dbReference type="PaxDb" id="273116-14325531"/>
    <dbReference type="GeneID" id="1441409"/>
    <dbReference type="KEGG" id="tvo:TVG1333406"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_1_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="PhylomeDB" id="Q977Y0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:3-methylbutanoyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="9"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="122"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="223"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="382" mass="42587" checksum="E71645D9E64158F2" modified="2001-10-01" version="1">MHSYVDADDLLRNSIKEFVKKEVEPIRMKIDREDYFPVDVFKEMGKLGYLGVTIPQEYGGSDAGYITQAVIEEELGYSSPSLALSYGAHSNLCLDNVYRNGSQAIREKFVPKLASGEWIGSLCLTEPGSGSDALAMHTTLHDHGGRIFLTGSKTFITNGPYADLFLVHAKDGDSYTSVIVLSSDKGFERGKKFEKMGMRGSPTGELFFNDIDIGNERIVGKRGDGKRIIMSGLNSERVILAFIFVGLARRALDEAVKYSSERKQFSLPISEFELIQEKLAYMYTKYETSRILAYRALEKLESSRDDPLYAAAAIMHASESAEYIAREAIQIFGGYGYIKDTGIEMFLRDAILGQIGAGTTEIRKRIIAKSLIRKYKLGEDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q977Z5</accession>
    <name>Q977Z5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1579590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917751.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q977Z5"/>
    <dbReference type="SMR" id="Q977Z5"/>
    <dbReference type="STRING" id="273116.gene:9382337"/>
    <dbReference type="PaxDb" id="273116-14325761"/>
    <dbReference type="DNASU" id="1442211"/>
    <dbReference type="GeneID" id="1442211"/>
    <dbReference type="KEGG" id="tvo:TVG1579590"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_0_1_2"/>
    <dbReference type="OrthoDB" id="46008at2157"/>
    <dbReference type="PhylomeDB" id="Q977Z5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF22">
        <property type="entry name" value="CONDITIONED MEDIUM FACTOR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="374" mass="41064" checksum="4F2296318A251C20" modified="2001-10-01" version="1">MKDVIVIGAGPSGSYAAYRLSKMGLDVLLLEEHKEVGKPVECTGLVSKRVLDFVKTRSIAGKVYGANIYFPNGKSIHVEKNDETIVLYRDSFDKDVAAMAIGAGADIRINARAISVKENNDSVTVKYRDSGQLKEDNAKVVIGADGINSVVRRDLYRIRQEKVVSTLQYDMAGKIEDERSVNVYLGSKYTHGFFGWAVPGGEIIRVGTGSYRASAYPYMKALSKRFGTNRVLGITGAGIPIKIAKSLFTKRSVLIGDAGGIVKPLSGGGIYTGIFSANLASERVANAIENEDYSQLRHYDKDIRGSIGVELKKDGFIQSIYSKIGDRSFDRIYGLISSEKIIDTINRSGDIDYPSKVIFKVLIRNPALLYSMWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q977Z8</accession>
    <name>Q977Z8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamine synthetase</fullName>
            <ecNumber evidence="3">6.3.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamate--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">TVG1571797</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="20 21" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16 17">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q977Z8"/>
    <dbReference type="STRING" id="273116.gene:9382334"/>
    <dbReference type="PaxDb" id="273116-14325758"/>
    <dbReference type="KEGG" id="tvo:TVG1571797"/>
    <dbReference type="eggNOG" id="arCOG01909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_3_2"/>
    <dbReference type="PhylomeDB" id="Q977Z8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF12">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="18">
        <location>
            <begin position="33"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="19">
        <location>
            <begin position="125"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
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</entry>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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</entry>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawamoto T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    <dbReference type="GeneID" id="1442179"/>
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    <dbReference type="eggNOG" id="arCOG00885">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008785_0_0_2"/>
    <dbReference type="OrthoDB" id="75806at2157"/>
    <dbReference type="PhylomeDB" id="Q978C8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
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    </dbReference>
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        <property type="entry name" value="Helicase_C-like"/>
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    </dbReference>
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        <property type="entry name" value="ATP-DEPENDENT HELICASE IRC3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME ECOKI R PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="1">
        <location>
            <begin position="357"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="843" mass="98887" checksum="2D3F74B86ABF8940" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q978D9</accession>
    <name>Q978D9_THEVO</name>
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        <submittedName>
            <fullName evidence="6">Formate hydrogenlyase subunit 5</fullName>
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        <name evidence="6" type="ORF">TVG1529492</name>
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    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978D9"/>
    <dbReference type="STRING" id="273116.gene:9382292"/>
    <dbReference type="PaxDb" id="273116-14325717"/>
    <dbReference type="GeneID" id="1442169"/>
    <dbReference type="KEGG" id="tvo:TVG1529492"/>
    <dbReference type="eggNOG" id="arCOG01545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_2_0_2"/>
    <dbReference type="OrthoDB" id="15253at2157"/>
    <dbReference type="PhylomeDB" id="Q978D9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ComplexI_Subunit1"/>
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        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="6"/>
            <end position="27"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="253"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="314" mass="35062" checksum="2DA07090F36892ED" modified="2001-10-01" version="1">MGAITLIETLMQYFFVVLTAPLYTGILEKLKAMVESRKGPSIFQPYYDLWKLSRKITVIPAGSGIFFRYIPYVLFGIYSLIALIIPVVIPVPIYFTASADFLGGAILFSLAAFMKAAAAMNSGSNYVALGVSRSMSFNFLSEGTLITVFIAVSLLTATNNPYITNAFLASHPLANLSLAHIFSTLAFFMLFFYETGKIPLESSGVQELGMIDEGLNYEYSGKLLAINKWSSYIKQYLLGSVLLNVFLFPWGLFSTYPYFFIDVPVMMAKWLLLILIVLVVETTLAKLRLFRVMDYLATAFTFSILFLIFAEVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q978G1</accession>
    <name>Q978G1_THEVO</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978G1"/>
    <dbReference type="STRING" id="273116.gene:9382266"/>
    <dbReference type="PaxDb" id="273116-14325693"/>
    <dbReference type="GeneID" id="1442143"/>
    <dbReference type="KEGG" id="tvo:TVG1506196"/>
    <dbReference type="eggNOG" id="arCOG02125">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079271_0_0_2"/>
    <dbReference type="OrthoDB" id="56498at2157"/>
    <dbReference type="PhylomeDB" id="Q978G1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="278" mass="33022" checksum="BADDCD7163042CD3" modified="2001-10-01" version="1">MIVMLNSEDRKFIIDSVKRGYKVSELAKMFNVTPRRIQQILHESASPDSEKDSELTEDEKRFIDELWDKYKIGSRTIYYLLRSKGMNVSYYRIYNYMKFKRMVHMKADALIINGKEADPPLTTVLMDYHQKNLNDPYAIFCVDMTTKKILSYAESLKITSDVVSKVIDNLYTGNVKIKHLMIRSGVLSLIYNTSYVSYRIRRKGIQDVVTDKNGSKVHLSLSKLWQNYDRYRWTFQSIDDFVHWYNERPVTRFEDRIASPGQIFEEYIRKFEMQRGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q978H0</accession>
    <name>Q978H0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1493094 protein</fullName>
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        <name evidence="2" type="ORF">TVG1493094</name>
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    <organism evidence="2 3">
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            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917675.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978H0"/>
    <dbReference type="PaxDb" id="273116-14325684"/>
    <dbReference type="GeneID" id="1442133"/>
    <dbReference type="KEGG" id="tvo:TVG1493094"/>
    <dbReference type="eggNOG" id="arCOG06915">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313950_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="99" mass="11350" checksum="05A693383BF80658" modified="2001-10-01" version="1">MVRGIDETNSQDEPKNTAIQDEKTEKTENQLTFRKIITDDKRHNPVPSKYKPGMYVAMTISEARQIKAYNFSSEVSEREFKAMADEPQKKIAIGKGWSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q978J6</accession>
    <name>Q978J6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1466613</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917651.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978J6"/>
    <dbReference type="STRING" id="273116.gene:9382228"/>
    <dbReference type="PaxDb" id="273116-14325658"/>
    <dbReference type="GeneID" id="1442109"/>
    <dbReference type="KEGG" id="tvo:TVG1466613"/>
    <dbReference type="eggNOG" id="arCOG01845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_2_2_2"/>
    <dbReference type="OrthoDB" id="371709at2157"/>
    <dbReference type="PhylomeDB" id="Q978J6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052339">
        <property type="entry name" value="Fe-S_Maturation_MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831:SF1">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="100" mass="11222" checksum="FF24C43ACA05D73F" modified="2001-10-01" version="1">MVTKEEILEALKTVSDPEIGYDVVNLGLVYDVEINGNRVYIRMTMTAPTCPVTPWILSQAQQVVEELPGVEACDIELVWDPPWSPKMMSDEAKQALNIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q978J7</accession>
    <name>Q978J7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1466300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917650.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978J7"/>
    <dbReference type="STRING" id="273116.gene:9382227"/>
    <dbReference type="PaxDb" id="273116-14325657"/>
    <dbReference type="GeneID" id="1442108"/>
    <dbReference type="KEGG" id="tvo:TVG1466300"/>
    <dbReference type="eggNOG" id="arCOG01691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078469_3_1_2"/>
    <dbReference type="OrthoDB" id="9623at2157"/>
    <dbReference type="PhylomeDB" id="Q978J7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="215" mass="24540" checksum="F4C357C65A1C6182" modified="2001-10-01" version="1">MLYDDILGDTKAKILEKLNISDLSLDQLSEILKINKTAVKEHMEFLEMRGYVSSYFKPSGTGRPKKFYKLTDKGISLFPKKYIEFTKLLVEEVEKDVGSYKLNGMLERIAAKIISDTGWSSGDLRDMPRDEKIRKLEEYVNMLNVLGYNATIDVYSDRVVISRHNCIFYDLAKTNNKIICNSLGKDLVLQSLNADFEITERISSGSSKCVIEVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q978K3</accession>
    <name>Q978K3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pyruvate dehydrogenase E3 / dihydrolipoamide dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1458424</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917644.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978K3"/>
    <dbReference type="STRING" id="273116.gene:9382221"/>
    <dbReference type="PaxDb" id="273116-14325651"/>
    <dbReference type="GeneID" id="1442102"/>
    <dbReference type="KEGG" id="tvo:TVG1458424"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_2_2"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="PhylomeDB" id="Q978K3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004943">
        <property type="entry name" value="PRK06292.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="5">
        <location>
            <begin position="338"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="450" mass="49587" checksum="2A6BBEEECAF9CFE9" modified="2001-10-01" version="1">METYDVITLGAGGAAYPAAFRLKKSGYSVLMIDKKGIMSGNCLAEGCVPSKAIIETVHNYSKLKKFGKYSIAYEDIVKHKDDVQNIRYKQHDEELHEAGLKILKGTAKLIDENTVEVETDTGKERYRAESIIIGTGSDTYVPKIPGAELAWTSRELYALNPKVRKLPKSIAIIGGGYIGLETASFMSLLGTDVTVIELLDRVLSTMDSGMVSKLVPLLPKMNLILSSPVKEIRRGGDMFEVSYDHEGKTEKIRVEAVLMAVGRMPMFPEGLDDLGIKYDKHGIKVNMAMQTNIKHIYATGDVNGLTPLFHAAKRQSLVAANNIMANHVPVDYFDPLSVPFTVYTVPQVAYVGILPDQAKKLGISYIETDYQMEKDAMAQVNSEMEGEIRLFFDSRMKIIGGYVIGNDAGNLINEIALGISKGLSARDFAEMAHQHPMSFEGLDSAARKLY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q978N3</accession>
    <name>HUTI_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazolonepropionase</fullName>
            <ecNumber evidence="1">3.5.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Imidazolone-5-propionate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hutI</name>
        <name type="ordered locus">TV1382</name>
        <name type="ORF">TVG1430123</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolytic cleavage of the carbon-nitrogen bond in imidazolone-5-propanoate to yield N-formimidoyl-L-glutamate. It is the third step in the universal histidine degradation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-imidazolone-5-propanoate + H2O = N-formimidoyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23660"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
            <dbReference type="EC" id="3.5.2.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc or iron ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. HutI family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.7" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917615.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978N3"/>
    <dbReference type="SMR" id="Q978N3"/>
    <dbReference type="STRING" id="273116.gene:9382190"/>
    <dbReference type="PaxDb" id="273116-14325621"/>
    <dbReference type="GeneID" id="1442073"/>
    <dbReference type="KEGG" id="tvo:TVG1430123"/>
    <dbReference type="eggNOG" id="arCOG00696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041647_1_0_2"/>
    <dbReference type="OrthoDB" id="24954at2157"/>
    <dbReference type="PhylomeDB" id="Q978N3"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00551"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050480">
        <property type="term" value="F:imidazolonepropionase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01296">
        <property type="entry name" value="Imidazolone-5PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00372">
        <property type="entry name" value="HutI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005920">
        <property type="entry name" value="HutI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01224">
        <property type="entry name" value="hutI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752:SF1">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0369">Histidine metabolism</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000160980" description="Imidazolonepropionase">
        <location>
            <begin position="1"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>N-formimidoyl-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00372"/>
        </source>
    </evidence>
    <sequence length="404" mass="44193" checksum="E76581A3B3715479" modified="2001-10-01" version="1">MKITNIGAIVTPVGNSYHSGDKQSELDFIENATIYIENGKIAKITREHSSDAGDLDAEGSIVIPGLVDSHTHIIFGGNRSQEFYQRASGYTYSEILRSGNGIYKTVRDTANSSADEIFTQSMKRIADAVSHGTTTIELKTGYGLYEKEERKLLDVARKISNSGLVSTVLTYLMHVLPEGESEEAYEKYSEKILSSFRNYISFADVFCDEGAFSPNAAKAFLKFADNLGLGLKIHANEINNIGCVKACSGLNVKSFDHMIHANAEDVETIRSIGSAITLLPLTVFALDESYPDARVFIDSGVPVIIASDISPLNYNANLIFAMHLAVKYSSMKPEEALTATTINAASSLGLGEKKGTVEEGKDADIVIIDVDDYTEIPYEYGINTVKKVFHNGSLVFDRTKQFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q978P3</accession>
    <name>Q978P3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carboxymuconolactone decarboxylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1420367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917606.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978P3"/>
    <dbReference type="STRING" id="273116.gene:9382180"/>
    <dbReference type="PaxDb" id="273116-14325611"/>
    <dbReference type="GeneID" id="1442064"/>
    <dbReference type="KEGG" id="tvo:TVG1420367"/>
    <dbReference type="eggNOG" id="arCOG02148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137228_2_2_2"/>
    <dbReference type="OrthoDB" id="111898at2157"/>
    <dbReference type="PhylomeDB" id="Q978P3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33930">
        <property type="entry name" value="ALKYL HYDROPEROXIDE REDUCTASE AHPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33930:SF2">
        <property type="entry name" value="BLR3452 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxymuconolactone decarboxylase-like" evidence="1">
        <location>
            <begin position="22"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="120" mass="13278" checksum="4CA156ABF04EFE43" modified="2001-10-01" version="1">MDPMERMTEIQKVFSASSKIKPDLTESFLSFMEKTLGKGELDVKTKSLVALALSLGLQCEWCITYHTKKCLEAGCTESEMMETAYIAAIMAGTPALMNVNIMEEAIRGFNQTNSDSDISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q978P6</accession>
    <name>Q978P6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gfo/Idh/MocA family oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1418453</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917603.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978P6"/>
    <dbReference type="STRING" id="273116.gene:9382177"/>
    <dbReference type="PaxDb" id="273116-14325608"/>
    <dbReference type="GeneID" id="1441485"/>
    <dbReference type="KEGG" id="tvo:TVG1418453"/>
    <dbReference type="eggNOG" id="arCOG01622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_4_2"/>
    <dbReference type="OrthoDB" id="25239at2157"/>
    <dbReference type="PhylomeDB" id="Q978P6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="2">
        <location>
            <begin position="132"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36706" checksum="59F7D71278417259" modified="2001-10-01" version="1">MDVVVVGCKGFGKEHLRVYSKNGIDVAIVERDSKTAQECVEKFNIKKVYSNFNDSLSSNANVFDLVVPDGLHHDMAFTLMKLKKHVLLEKPITPDIDSGIDLIKTSNEQSVKFMVAEQFYFDPTVKFIINEIKKGTIGKAHTVIVRHQSHRKLEGWRKESRMIGRGILINEGTHYIDTLLNIGGRYSSLRSYVHRTWESAGEDTAEAVFNFENGMHGVFFYSWNYPHAPELPEFEIIGDSGSIYEDVKTKPTGEWSDRKRRTAYGDPVVNGNHVNIEIYDVIEKEIMDFLNSVEKNEPVPMDPELALRDLITIDRIYKASSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q978P7</accession>
    <name>Q978P7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-acyl carrier protein reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1417421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917602.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978P7"/>
    <dbReference type="STRING" id="273116.gene:9382176"/>
    <dbReference type="PaxDb" id="273116-14325607"/>
    <dbReference type="GeneID" id="1441484"/>
    <dbReference type="KEGG" id="tvo:TVG1417421"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_2"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="PhylomeDB" id="Q978P7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005056">
        <property type="entry name" value="PRK06463.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="255" mass="28147" checksum="365DEC1B7A61FBC9" modified="2001-10-01" version="1">MSGKLEGKTALITGASKGLGRAIAEEFAKEGARVAINYAHDEKSALEVKKITGGEIFKADVSNRDQVKEMARQIHEKLGKIDILVNNSGIWYLMPFENYDEEKVRRMLDVNLMGAIYTTLEFLPDLKEKKGTIINIGSNAGIGTAAKNTTFYSLTKAAIIMLTKRLAFDLSEYSIRVNAIAPGWIETDLTIGGKTDEEIRKLEEDFKSRTTLKMFGKPEYIGKAALFLASSDSVYMNGQVIVIDGGRIDNLTHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Q0</accession>
    <name>Q978Q0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1413232 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1413232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Q0"/>
    <dbReference type="STRING" id="273116.gene:9382173"/>
    <dbReference type="PaxDb" id="273116-14325604"/>
    <dbReference type="KEGG" id="tvo:TVG1413232"/>
    <dbReference type="eggNOG" id="arCOG07198">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567245_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="178" mass="19462" checksum="6861F45C718697CC" modified="2001-10-01" version="1">MQFYHFFHMRKKIVLIGVVVLVIGIVIFAGGDAAILHYTVTDTAVKELATGQYGREITLTSSSLFTVSSTFPNWIVVTSSNYSKVTSGNYATYAISPIENTHVSSLYVKGYDLGTGSYYLLAFNSSQPPVTYSYLPLSVSYLSLLSVFGIVLLIIGIIIAIIGVILKRKEKTGDEIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q978R4</accession>
    <name>Q978R4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1395987 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1395987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917585.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978R4"/>
    <dbReference type="STRING" id="273116.gene:9382159"/>
    <dbReference type="PaxDb" id="273116-14325590"/>
    <dbReference type="GeneID" id="1441467"/>
    <dbReference type="KEGG" id="tvo:TVG1395987"/>
    <dbReference type="HOGENOM" id="CLU_2152755_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="111" mass="13267" checksum="F4E266C43B234218" modified="2001-10-01" version="1">MRQIQVIEKEEEEEVTEGKQLTTRQKLEYIFGDFLRGFYLIGCIFFDVLIVGFSLTFIPNVSIYEDVVYQSIGTNLILIYGLLLIIFTEAILIYYEIVWFKKFFLRKDHYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q978R9</accession>
    <name>Q978R9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1393408 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1393408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054029.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978R9"/>
    <dbReference type="STRING" id="273116.gene:9382154"/>
    <dbReference type="PaxDb" id="273116-14325585"/>
    <dbReference type="GeneID" id="24779969"/>
    <dbReference type="KEGG" id="tvo:TVG1393408"/>
    <dbReference type="HOGENOM" id="CLU_2519967_0_0_2"/>
    <dbReference type="OrthoDB" id="57273at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="84" mass="9773" checksum="005570D8F807D612" modified="2001-10-01" version="1">MDDKCIEIDNFPEEKKFKIKLEAALILDAQRIKALYNERKKFDDYIDERLNIIKNMLGIEGDFAVKDGDKEIIVVSKGEIKINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q978S0</accession>
    <name>Q978S0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 15">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="15">6.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">rtcB</name>
        <name evidence="16" type="ORF">TVG1391663</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for tRNA splicing and maturation. Acts by directly joining spliced tRNA halves to mature-sized tRNAs. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="14 15">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 15">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.-" evidence="15"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978S0"/>
    <dbReference type="STRING" id="273116.gene:9382153"/>
    <dbReference type="PaxDb" id="273116-14325584"/>
    <dbReference type="KEGG" id="tvo:TVG1391663"/>
    <dbReference type="eggNOG" id="arCOG04246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022279_0_1_2"/>
    <dbReference type="PhylomeDB" id="Q978S0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1860.10:FF:000001">
        <property type="entry name" value="tRNA-splicing ligase RtcB homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01288">
        <property type="entry name" value="UPF0027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="12">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="201"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="320"/>
            <end position="321"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="367"/>
            <end position="370"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="393"/>
            <end position="396"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU371113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAB60487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="469" mass="51538" checksum="A90870A88C99D4D6" modified="2001-10-01" version="1">MLIMNIRKIDDYTYEIPREGDMIVHGIVYSTDKLFKNTVEDGSLNQVVNVAKLPGIVEASYAMPDIHLGYGFPIGGVAAFDYDEGIISPGGVGYDINCGVALLRTGMNYAQIKPRIKDITDYIFQEVPSGLTSRKGFPVNQNDLQEILTSGLKWAVSRGLATELDMINTEDNGSIESHGTHVSKQAMQRGLSQIGTLGAGNHFLEIQRVSDIFDEDVARKFGLYKDEVTVMIHTGSRGLGHQVATDYLRELRESRESIKTSDPELISIHVKSKIGENYLDAMKSAANFAFVNRQMAIYGVRKVFKKFFDVEPELVYSLAHNIAKVEDHIVEGKTKSLIVHRKGATRAFGPGKSSPPFEDTGHPVLIPGSMGTASYVLVGVKENLIKSFGTACHGSGRVLSRNQAIKKFSSIVDRELEEKDVYARPATKRVLYEEAPGSYKNVDEVVAAVEGAHLARSIVRMVPLAVVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q978U0</accession>
    <name>Q978U0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alpha-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1365594</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978U0"/>
    <dbReference type="STRING" id="273116.gene:9382133"/>
    <dbReference type="CAZy" id="GH31">
        <property type="family name" value="Glycoside Hydrolase Family 31"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325564"/>
    <dbReference type="KEGG" id="tvo:TVG1365594"/>
    <dbReference type="eggNOG" id="arCOG03663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000631_7_2_2"/>
    <dbReference type="PhylomeDB" id="Q978U0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06604">
        <property type="entry name" value="GH31_glucosidase_II_MalA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF120">
        <property type="entry name" value="HETEROGLYCAN GLUCOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Galactose mutarotase-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="6">
        <location>
            <begin position="120"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="5">
        <location>
            <begin position="286"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="7">
        <location>
            <begin position="614"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="791" mass="91031" checksum="FC3DB237620DD8DD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Y3</accession>
    <name>Q978Y3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mutator protein [MutT]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1322048</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917516.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Y3"/>
    <dbReference type="STRING" id="273116.gene:9382088"/>
    <dbReference type="PaxDb" id="273116-14325520"/>
    <dbReference type="GeneID" id="1441398"/>
    <dbReference type="KEGG" id="tvo:TVG1322048"/>
    <dbReference type="eggNOG" id="arCOG01072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040940_5_3_2"/>
    <dbReference type="OrthoDB" id="40462at2157"/>
    <dbReference type="PhylomeDB" id="Q978Y3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
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    </dbReference>
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        <property type="entry name" value="CoAse"/>
    </dbReference>
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        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF11">
        <property type="entry name" value="MITOCHONDRIAL COENZYME A DIPHOSPHATASE NUDT8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="154" mass="17888" checksum="E24136415E51FA95" modified="2001-10-01" version="1">MRQPEAAVGLIVQRSEVLFIKRKARPDDPWSGDIAFPGGFLKEMESPAQAVIREIKEEVSLYFTEYDILAEMPLHYPISKQLPVHPFIIKSYSFDDALPGDEVDEIKIADIGDLVKDYDEKKGNIFRFGDWIIWGLTYRILNTYITRYYGNFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q979B4</accession>
    <name>Q979B4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TVG1286418 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1286418</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979B4"/>
    <dbReference type="STRING" id="273116.gene:9382052"/>
    <dbReference type="PaxDb" id="273116-14325485"/>
    <dbReference type="KEGG" id="tvo:TVG1286418"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_4_0_2"/>
    <dbReference type="PhylomeDB" id="Q979B4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050639">
        <property type="entry name" value="SSR_resolvase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30461">
        <property type="entry name" value="DNA-INVERTASE FROM LAMBDOID PROPHAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30461:SF2">
        <property type="entry name" value="SERINE RECOMBINASE PINE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="3">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="113" mass="13225" checksum="F4D1652821DA5A90" modified="2001-10-01" version="1">MNGYTISAIYAHIASGISLEKRKGFFEMLDEIINNKVEKVIITYKDRLSRVGFDLFSYLFRKYRTEIAVISEVGNTKLDSEEVFEEIVSMLHSYSMKMYSKRKNHYIEVGYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q979H1</accession>
    <name>Q979H1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1219926 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1219926</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917423.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979H1"/>
    <dbReference type="STRING" id="273116.gene:9381991"/>
    <dbReference type="PaxDb" id="273116-14325428"/>
    <dbReference type="GeneID" id="1441305"/>
    <dbReference type="KEGG" id="tvo:TVG1219926"/>
    <dbReference type="eggNOG" id="arCOG07371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922920_0_0_2"/>
    <dbReference type="OrthoDB" id="55880at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="131" mass="15427" checksum="AC690927DDE3CCC7" modified="2001-10-01" version="1">MPERAVEMVRREGDRYFVRLSVETAVYTSLEGKPDEFLLLTIVGSYDPKLKMLKQKINEKAICRTSTFKDSRGFDHPSVIIPAEIVREYKIEEGEYLELLIEHIVKEDKIAAIYPNLEVLQSYPYWWRKIT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q979K0</accession>
    <name>RPO10_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo10</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo10</name>
        <name evidence="1" type="synonym">rpoN</name>
        <name type="ordered locus">TV1161</name>
        <name type="ORF">TVG1188103</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917395.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979K0"/>
    <dbReference type="SMR" id="Q979K0"/>
    <dbReference type="STRING" id="273116.gene:9381961"/>
    <dbReference type="PaxDb" id="273116-14325399"/>
    <dbReference type="GeneID" id="1441277"/>
    <dbReference type="KEGG" id="tvo:TVG1188103"/>
    <dbReference type="eggNOG" id="arCOG04244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143122_2_1_2"/>
    <dbReference type="OrthoDB" id="371754at2157"/>
    <dbReference type="PhylomeDB" id="Q979K0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000121365" description="DNA-directed RNA polymerase subunit Rpo10">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00250"/>
        </source>
    </evidence>
    <sequence length="72" mass="8483" checksum="06AEC0AA7AC75CA6" modified="2001-10-01" version="1">MIIPVRCFSCGRVIASDYGRYLRRINEIRSEGREPTAEEIEKIFDDLGVERYCCRRMIISHVDLINEIMPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q979K7</accession>
    <name>Q979K7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TVG1184036 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1184036</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979K7"/>
    <dbReference type="STRING" id="273116.gene:9381954"/>
    <dbReference type="PaxDb" id="273116-14325392"/>
    <dbReference type="KEGG" id="tvo:TVG1184036"/>
    <dbReference type="eggNOG" id="arCOG01431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010224_0_0_2"/>
    <dbReference type="OrthoDB" id="371827at2157"/>
    <dbReference type="PhylomeDB" id="Q979K7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF19">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="4">
        <location>
            <begin position="2"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="123" mass="13246" checksum="2680EE990F549443" modified="2001-10-01" version="1">MMTDNSNPDNVDATRKLGGEVIFHGPKFNDARNYAEELAVKNGYRYFHSANEPLLVAGVGTHTPELIESDPEIDAIIVPVGDESGSRGAAIVAETMSKDIKVIGAQSEMFPAAYKARKSGVHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q979L0</accession>
    <name>Q979L0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-responsive regulatory protein [Lrp]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1179749</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="interaction">
        <interactant intactId="EBI-15665458">
            <id>Q979L0</id>
        </interactant>
        <interactant intactId="EBI-15665458">
            <id>Q979L0</id>
            <label>TVG1179749</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979L0"/>
    <dbReference type="DIP" id="DIP-29562N"/>
    <dbReference type="STRING" id="273116.gene:9381950"/>
    <dbReference type="PaxDb" id="273116-14325389"/>
    <dbReference type="KEGG" id="tvo:TVG1179749"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_3_2"/>
    <dbReference type="OrthoDB" id="14434at2157"/>
    <dbReference type="PhylomeDB" id="Q979L0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="IntAct"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.920:FF:000043">
        <property type="entry name" value="Leucine-responsive regulatory protein [Lrp]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000646">
        <property type="entry name" value="Transcriptional regulator, AsnC family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF4">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="130" mass="15198" checksum="8AF01E9937414AD9" modified="2001-10-01" version="1">MDEKDRDILNLLIDNSRISNTEIAKVLNVSEGTVRKRIKKMIDEGIIKRFTVETSDNTIDALILVKIDNKKYKEVLQRLRARYYEIYEFSGRIDVAIRIKTDSTDNLNRIVDQIREIEGVVDTDTLIRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q979P3</accession>
    <name>Q979P3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1149034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917351.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979P3"/>
    <dbReference type="STRING" id="273116.gene:9381916"/>
    <dbReference type="PaxDb" id="273116-14325355"/>
    <dbReference type="GeneID" id="1441233"/>
    <dbReference type="KEGG" id="tvo:TVG1149034"/>
    <dbReference type="eggNOG" id="arCOG01863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130237_0_0_2"/>
    <dbReference type="OrthoDB" id="11138at2157"/>
    <dbReference type="PhylomeDB" id="Q979P3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004451">
        <property type="entry name" value="MJ0586"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058562">
        <property type="entry name" value="MJ0586_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00270">
        <property type="entry name" value="multiprotein bridging factor aMBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26602">
        <property type="entry name" value="HVO_2718_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="88"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="143" mass="15970" checksum="A1F87B3DF1572A96" modified="2001-10-01" version="1">MECEMCGKKTQHTTKVMIDGAILNVCDDCAKFGTPVIEHNNFKRVESVKVTLPEPATRVPDTHPAKNAVKKKISEEDLDIVEDYPEIVKNAREKLGMSQADLAARIFERKNVIASIERGDLLPDIKTARKLEKILGITLLEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q979U1</accession>
    <name>Q979U1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1095254</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979U1"/>
    <dbReference type="STRING" id="273116.gene:9381864"/>
    <dbReference type="PaxDb" id="273116-14325307"/>
    <dbReference type="DNASU" id="1441180"/>
    <dbReference type="GeneID" id="1441180"/>
    <dbReference type="KEGG" id="tvo:TVG1095254"/>
    <dbReference type="eggNOG" id="arCOG01469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096331_0_0_2"/>
    <dbReference type="OrthoDB" id="97972at2157"/>
    <dbReference type="PhylomeDB" id="Q979U1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="ABC2_TM"/>
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    <dbReference type="PANTHER" id="PTHR43229:SF3">
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        <property type="entry name" value="NODULATION PROTEIN J"/>
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    </dbReference>
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        <location>
            <begin position="24"/>
            <end position="45"/>
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        <location>
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            <end position="74"/>
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        </location>
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        <location>
            <begin position="129"/>
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        </location>
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        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="6">
        <location>
            <begin position="21"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="247" mass="26745" checksum="D111E9EDB44ACBFE" modified="2001-10-01" version="1">MHTYRSSQLAASILVNAIYAMKNFPITLINTLLSPLSFLLVITLASHGRLISVSVGGALIMSMVSSGLGLQGDLSHLKNDFKLQDIVVSSPTTASVYLVGMAISEIVYSIPAISVLGILAYFFIHTSAIGVLTIIGVLVVMFSFSILLGFTLATITYDIIQSWAFSGIVSTLISTIPPVYYPITYIPMPFRYAAYASPTTYAAEIIQNSIGMISLSSTQIAEDWIIIVIVTILLLIIAVKKAKWREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q979U8</accession>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmata</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="PaxDb" id="273116-14325300"/>
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    <dbReference type="KEGG" id="tvo:TVG1086877"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="56009at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="198" mass="21728" checksum="1CEF8EBFC7C74722" modified="2001-10-01" version="1">MTKASEAVKTIVSNNNVYSLLINTGLVNYTKLAREIKGQVDFLTGKKIKINTIVKALTNIEVKGKNSNALEILKQSTLTLEYNYEEQHFENPEEIPKSAILIIKDQNGYSTLAKSAKSNSLVIAKIHLPAGSCTTAGITLLITEFLEINGISVVNIYRLSREVWIIIQSESAGKAADALNRLLFQNEKNVGGPTIEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q979V8</accession>
    <name>Q979V8_THEVO</name>
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            <fullName evidence="1">anthranilate synthase</fullName>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
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        <property type="protein sequence ID" value="BAB60194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917281.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979V8"/>
    <dbReference type="STRING" id="273116.gene:9381846"/>
    <dbReference type="MEROPS" id="C26.955"/>
    <dbReference type="PaxDb" id="273116-14325290"/>
    <dbReference type="GeneID" id="1441163"/>
    <dbReference type="KEGG" id="tvo:TVG1078652"/>
    <dbReference type="eggNOG" id="arCOG00086">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_2_2"/>
    <dbReference type="OrthoDB" id="3321at2157"/>
    <dbReference type="PhylomeDB" id="Q979V8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="183" mass="20595" checksum="520B25636B3FED9C" modified="2001-10-01" version="1">MKMLVVDNHDSFVYNLYYYLREMNIEVDVVENDKLVDASGYDKIIISPGPGSPLNARDRGRTLDVVDKFDGPVLGICFGHQLLAYHLGSQISVARRPYHGEIDRIKHNGSSLYLGIPDSFDAIRYHSLVISPNKNIEVDAVSETDGNIMGFHSVDGRIYGIQFHPESYFTSVGKQILKNFVML</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q979W1</accession>
    <name>Q979W1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12 13">Pyruvate kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">TVG1076108</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="3 12 13"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979W1"/>
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    <dbReference type="PaxDb" id="273116-14325287"/>
    <dbReference type="GeneID" id="1441160"/>
    <dbReference type="KEGG" id="tvo:TVG1076108"/>
    <dbReference type="eggNOG" id="arCOG04120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_2_2"/>
    <dbReference type="OrthoDB" id="56298at2157"/>
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    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
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        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="14">
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            <begin position="2"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="15">
        <location>
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            <end position="448"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR01064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAB60191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="541" mass="58810" checksum="A2F5CB05CC39D2FF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q979W7</accession>
    <name>Q979W7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TVG1068999 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1068999</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="KEGG" id="tvo:TVG1068999"/>
    <dbReference type="eggNOG" id="arCOG01811">
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="ArlJ-like"/>
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    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
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        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF1">
        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="5"/>
            <end position="20"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="186"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="212"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="59"/>
            <end position="182"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="245" mass="27551" checksum="E9373B63B61CAF2E" modified="2001-10-01" version="1">MKPIYGAVPALLAVVLYLIYRNPAVILALIASGIAIAFGSEYIDLRRNEEKRNMMVPSFLRDLSSFSSYGMSMYNILHLILSEDLGPLNEDVRNARAILDNGNTIEEVSAYLRSRNSRDLDIVAEVMKNADRAGRPGTTLQFLSTYMNERMSQRKNATQTAENYVGLIIASFMIFLLVMIVVDYYFIQRSGFLLIDYISAILIIQSIFTGFYTGLVRYSSIRSGLFYSGMLVTITVTLLTVARLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q979X7</accession>
    <name>Q979X7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Elp3/MiaA/NifB-like radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1057056</name>
    </gene>
    <organism evidence="8 9">
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        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917262.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979X7"/>
    <dbReference type="STRING" id="273116.gene:9381826"/>
    <dbReference type="PaxDb" id="273116-14325271"/>
    <dbReference type="GeneID" id="1441144"/>
    <dbReference type="KEGG" id="tvo:TVG1057056"/>
    <dbReference type="eggNOG" id="arCOG01360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060488_0_0_2"/>
    <dbReference type="OrthoDB" id="105445at2157"/>
    <dbReference type="PhylomeDB" id="Q979X7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005909">
        <property type="entry name" value="RaSEA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01210">
        <property type="entry name" value="archaeosine biosynthesis radical SAM protein RaSEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004954">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Elp3/MiaA/NifB-like radical SAM core" evidence="7">
        <location>
            <begin position="43"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="350" mass="40392" checksum="BEC9BEEF82A241D4" modified="2001-10-01" version="1">MVNRELALFIKSLMPSYSSKVEKDKPVSIWNEMDRLRGYPEKSTVAILRTRGCSWYRFSACSMCGYFNDTADVSNENLFKQIDHLSSVLNTEVLKVFTSGSFLDPIEVPDEVRAYFYDSISNKVEKLLIESRTEYIRDERLEEIKKYGFETRIAIGLESANDYIVENSINKGSSFTKYVDAAKRIRNNGLELRTYLLLKPPFISEKSAIKDVLDSIRAVKDLTNDVSINPTNVQKNTLVEYLWKRGLYRPPRLWSLARVLIDGMKIGPEVISYPTGGNKERGIHNDEFDYDLLDLIVKSSLNQDTTELERYMKSQDLSKYMEELEIEDRSLFQCDFGKLIDQSLNSVAYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A01</accession>
    <name>Q97A01_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">2-dehydro-3-deoxyglucokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1031550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917237.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A01"/>
    <dbReference type="STRING" id="273116.gene:9381802"/>
    <dbReference type="PaxDb" id="273116-14325247"/>
    <dbReference type="GeneID" id="1441119"/>
    <dbReference type="KEGG" id="tvo:TVG1031550"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_5_2_2"/>
    <dbReference type="OrthoDB" id="26949at2157"/>
    <dbReference type="PhylomeDB" id="Q97A01"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01942">
        <property type="entry name" value="ribokinase_group_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.150.10">
        <property type="entry name" value="putative 5-dehydro-2- deoxygluconokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023314">
        <property type="entry name" value="Myo_inos_IolC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="6">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="289" mass="32256" checksum="F5D8DDA7FC5AE7BE" modified="2001-10-01" version="1">MAFLAYFGHLNIDVRILVENLPKEGSVNAKNVEEKYGGTAGNFAIVSKKLGLDFDIYSAVGSKTHSGYLSFLKDSGIGIDHIKVTDEYGPICYIASDTKKQVAYMYQGPMLNWSPRLESHYEYIHLSTGPKYTGLIENKESKLVFDPSQEIWKFSGEELKRFYEASYISFFNGNEMKVFREATGLKSFDRIVIETVGPRGSILYKDSTKKVFPALPSSGDTIGAGDAFRAGFYYALFRKKSIETAMVYGTIAAHHMIEDGIDGFSLDANSIEQESQVYKKTFGIQSTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A28</accession>
    <name>Q97A28_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1003975 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1003975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917211.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A28"/>
    <dbReference type="STRING" id="273116.gene:9381774"/>
    <dbReference type="PaxDb" id="273116-14325199"/>
    <dbReference type="GeneID" id="1442059"/>
    <dbReference type="KEGG" id="tvo:TVG1003975"/>
    <dbReference type="eggNOG" id="arCOG07395">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1014183_0_0_2"/>
    <dbReference type="OrthoDB" id="379158at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="290" mass="32199" checksum="269C97FB685A951C" modified="2001-10-01" version="1">MKYQDSAVSEIIGTVILFAIVVTLVTAYMSWYVPLQEERSQGEYYSETLSAITQFVSKTYQNSTFVQEFPSGIYSGIGSTADTAVSYSNSLTSSLNVTMQIELNASGKLKYVNETEAYTTAGILKSYPYTDFLKVETFNVYGNFISIDKTYYGPLNFNLSSNELRFNGVSLSSKGSVVSSPSSISVSGVPVESKIFQAVKGQILNLSGTKGIVEKITILSLNYTLNGSFSLSLFRYLSFALNGTIEANEDSFRYNNMTIKYAQNFLNVSSISSFTVNDFYINYRDFYIAF</sequence>
</entry>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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        <property type="project" value="UniProtKB-KW"/>
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    </evidence>
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    </evidence>
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        <source>
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    <sequence length="245" mass="27901" checksum="F44602DD38ED05E8" modified="2001-10-01" version="1">MSDNPFFKKFAEDYAKSKSHEKGQDLTELLGLINTHRDTCLDVATGTGFTAASISQICAHVTALDETRAMIEQAQSLIESRATKNVKFVLSTFEDFDDGKFDLITCRRALHHFKDKEAFFKKARTMLNPGGILAIADMVSPANDRKDNFNVLERIRDPTHIGALKVEEMIKLFEENNFQDIKYKIITEELTFEEWLYPITKESEVGKKCLEFLNSLSTEELNQIRLDANRLTLSKQRIVIAASVQ</sequence>
</entry>
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    <accession>Q97AB4</accession>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
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                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Nunoshiba T."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
            <end position="43"/>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1030"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <sequence length="1038" mass="117840" checksum="D464EB506CC0DCB2" modified="2001-10-01" version="1"> </sequence>
</entry>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AD8</accession>
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        <lineage>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
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                <person name="Yamamoto Y."/>
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                <person name="Amano N."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917116.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AD8"/>
    <dbReference type="STRING" id="273116.gene:9381664"/>
    <dbReference type="PaxDb" id="273116-14325089"/>
    <dbReference type="GeneID" id="1441964"/>
    <dbReference type="KEGG" id="tvo:TVG0890402"/>
    <dbReference type="eggNOG" id="arCOG02462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030671_3_2_2"/>
    <dbReference type="OrthoDB" id="9014at2157"/>
    <dbReference type="PhylomeDB" id="Q97AD8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118:SF12">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24363" checksum="111CA032046E023B" modified="2001-10-01" version="1">MFKVDRYFDLTAPIYPYMPVWPSNPPVELKPIVTVPKDGYLAEIIEASTHTGTHIDAPAHMIEGGTTVDDLDLASLIGTGYCLKVLPDGHTIRSTHLAEKWQGIYNNSILLIETGWYKKRGYTREFQYDFPGLSEDAADFLIQKHVKAVGIDTLGIEPYENTDFRVHKKLLRSGIPIIEDLYGLDSLEEGRPYLIIALPLRLKGTSGSMARVVAAETE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AG7</accession>
    <name>SYI_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="1">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ileS</name>
        <name type="ordered locus">TV0843</name>
        <name type="ORF">TVG0867938</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917087.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AG7"/>
    <dbReference type="SMR" id="Q97AG7"/>
    <dbReference type="STRING" id="273116.gene:9381635"/>
    <dbReference type="PaxDb" id="273116-14325060"/>
    <dbReference type="GeneID" id="1441935"/>
    <dbReference type="KEGG" id="tvo:TVG0867938"/>
    <dbReference type="eggNOG" id="arCOG00807">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_1_1_2"/>
    <dbReference type="OrthoDB" id="30823at2157"/>
    <dbReference type="PhylomeDB" id="Q97AG7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00818">
        <property type="entry name" value="IleRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000098596" description="Isoleucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="591"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="594"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02003"/>
        </source>
    </evidence>
    <sequence length="1028" mass="119494" checksum="ABBBCD972B46641F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AH6</accession>
    <name>Q97AH6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0853481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917078.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AH6"/>
    <dbReference type="STRING" id="273116.gene:9381626"/>
    <dbReference type="PaxDb" id="273116-14325051"/>
    <dbReference type="GeneID" id="1441926"/>
    <dbReference type="KEGG" id="tvo:TVG0853481"/>
    <dbReference type="eggNOG" id="arCOG05374">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077738_2_2_2"/>
    <dbReference type="OrthoDB" id="56134at2157"/>
    <dbReference type="PhylomeDB" id="Q97AH6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="10"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="140" mass="15617" checksum="0FEDE52B473C3C91" modified="2001-10-01" version="1">MDLNDFSIKVLQEVCRILKESNKKYDWVGVYVMENRKLKLEAFVGEKTEHEVINLGDGLCSLAVLKNEVVNEYDVKSNPKYLACFPSTQSEIVVPVRYGGKPIGEIDIDSDTKGAFDKADEEFLSKVADLIAPLVSSLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AJ0</accession>
    <name>Q97AJ0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6 8">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="5">DAP decarboxylase</shortName>
            <shortName evidence="5">DAPDC</shortName>
            <ecNumber evidence="5 6 8">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">lysA</name>
        <name evidence="11" type="ORF">TVG0827631</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>meso-2,6-diaminopimelate + H(+) = L-lysine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="5 6 8"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917064.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AJ0"/>
    <dbReference type="SMR" id="Q97AJ0"/>
    <dbReference type="STRING" id="273116.gene:9381610"/>
    <dbReference type="PaxDb" id="273116-14325036"/>
    <dbReference type="GeneID" id="1441912"/>
    <dbReference type="KEGG" id="tvo:TVG0827631"/>
    <dbReference type="eggNOG" id="arCOG02268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_0_2_2"/>
    <dbReference type="OrthoDB" id="18565at2157"/>
    <dbReference type="PhylomeDB" id="Q97AJ0"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000003">
        <property type="entry name" value="Diaminopimelate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43727">
        <property type="entry name" value="DIAMINOPIMELATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43727:SF2">
        <property type="entry name" value="GROUP IV DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 5 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="3 5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="10">
        <location>
            <begin position="41"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="270"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5 7">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="409" mass="45227" checksum="3DBCE5131E5E3E33" modified="2001-10-01" version="1">MENDNPFVWNGDDMLVEGYSVKAIVETFGTPVIIYSAPRIVKNVSSLFGAMPKVTKCFYSVKANSNPNLVRLIGRQGCGSEVTSLGEIQIAMISSIDPDDILFTPNNVSKEEMDFALDRGIKITFNSPGQFELVSNRVEEATFRINPGIGHGEHPGTTTGGHGSKFGIDPETALKLYTRAKELGIRKFGIHMMVGSNNTDADLMLTFYKKFFDMASEIEKTAHLQFSYIDIGGGFGVPYNDEQRSLDIKKLGSGLKELVDQYGIERLFIEPGRYIVADAGILVGKVYDVYNGFVGTDIGMNINIRHALYGAEHRFYALTGRSDEAEYIVTGQICENTDVTGRLTGKVQIGDLVAMYDAGAYIYSMSSNYNGRFRPTEVIVDHDGVYRIRRRETVNDILATVTGMEKVNV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-11" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AJ4</accession>
    <name>CARA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbamoyl phosphate synthase small chain</fullName>
            <ecNumber evidence="1">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Carbamoyl phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carA</name>
        <name type="ordered locus">TV0816</name>
        <name type="ORF">TVG0820736</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <molecule>Carbamoyl phosphate synthase small chain</molecule>
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917060.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AJ4"/>
    <dbReference type="SMR" id="Q97AJ4"/>
    <dbReference type="STRING" id="273116.gene:9381606"/>
    <dbReference type="PaxDb" id="273116-14325032"/>
    <dbReference type="GeneID" id="1441908"/>
    <dbReference type="KEGG" id="tvo:TVG0820736"/>
    <dbReference type="eggNOG" id="arCOG00064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035901_2_1_2"/>
    <dbReference type="OrthoDB" id="7675at2157"/>
    <dbReference type="PhylomeDB" id="Q97AJ4"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF7">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000112368" description="Carbamoyl phosphate synthase small chain">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="168"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <sequence length="345" mass="37923" checksum="932607F0284B24F7" modified="2001-10-01" version="1">MKKYLVMEDGTVLSGQGYGSYKEAYGELVFTTSMTGYLETLTDPSYVGQMLVFASPTIANYALEMGKMESNGVKVSALITKDAHIELKKGGYGREFDEFLKEQGIPGIDGIDTRMLVRKIRANGVLRSYVVNDPSHSLDFPDPMNDDLVSKAVPHHDPVFVKGEEGGTVLFIDLGSKNTLKELMLQNFSLIVVDKQTNLDEIEGYDAIFVSNGPGDPSHPSLSNVIKFLSHNIGVKPLFGICFGLQAISLAYGAKTYKMKFGHRGSNHAVTDGTHSYVTTHNHGYAVDGSTVKDFKVIGWDANDGTVEIIEGDDMFAVQFHPEASPGPHDTRWFFGEMKRRIGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AK3</accession>
    <name>Q97AK3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adenosylcobinamide amidohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0808276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AK3"/>
    <dbReference type="STRING" id="273116.gene:9381597"/>
    <dbReference type="PaxDb" id="273116-14325023"/>
    <dbReference type="KEGG" id="tvo:TVG0808276"/>
    <dbReference type="eggNOG" id="arCOG01870">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1259167_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AK3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002808">
        <property type="entry name" value="AdoCbi_amidolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052209">
        <property type="entry name" value="CbiZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336:SF5">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01955">
        <property type="entry name" value="CbiZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24206" checksum="FFF73510A746FF1D" modified="2001-10-01" version="1">MLMLISFDFLEENDYYMLKFHSEARKFSSAPFNGGVGRAKAYINRTVNLDYNEEVISETKEFLARNKIEENGTVCTLTAVDVNTHVVAKKYLDTNSLIVYITAGFDNTVSIGNVKGMHGTINIALLTDFPLSDAGVLNLFQTIVESKAQFMNDAHVTDRATGKVGPGTSTDTVSVFVFNDEKTINYAGRITAIGHEASLMVYNSLLKINENNKKAHEMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AN4</accession>
    <name>Q97AN4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0780822</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AN4"/>
    <dbReference type="STRING" id="273116.gene:9381566"/>
    <dbReference type="PaxDb" id="273116-14324992"/>
    <dbReference type="KEGG" id="tvo:TVG0780822"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521955_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AN4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="7"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="176" mass="19617" checksum="0C82E2BEA61E8483" modified="2001-10-01" version="1">MVSPGGAGLSYAAYPARIFQSMAEFGYAPKWFKELSKKKVPARALILGFFVGLVFLYEFPGWDLLLGILTSTLVIGYVMGPASINILRKHAPNVERMFMLRGSSFIAPIAFIFSSLIIYWSGWPLSGEVVVAVIAGLFMFSYFTHVNDFDWVEIRSGMWLVALLFVIAVLSGYDFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AP0</accession>
    <name>Q97AP0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0773051</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917017.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AP0"/>
    <dbReference type="STRING" id="273116.gene:9381560"/>
    <dbReference type="PaxDb" id="273116-14324986"/>
    <dbReference type="GeneID" id="1441865"/>
    <dbReference type="KEGG" id="tvo:TVG0773051"/>
    <dbReference type="eggNOG" id="arCOG01352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_2"/>
    <dbReference type="PhylomeDB" id="Q97AP0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000003">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="202"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="435" mass="47737" checksum="8F5C570B3740D597" modified="2001-10-01" version="1">MSGKADPLEIAKEQVAPVKEEIEEDPFEIALKQLNKAAKVLNLDEQALEILSSPQKILQVSIPVKMDDGKVRVFTGFRVRHNIARGPAKGGIRYHPQETLSTVKALSMWMTWKCAIADIPYGGAKGGIICDPSTLSQGELERLSRGYIRAIADFIGPEVDVPAPDVNTNPQIMAWMMDEYENIVRHSAANVITGKPLEVGGSLGRFDSTGKGGMFVLREGAKKIGLDLSKARVAVQGFGNVGQFAVKFVQEMFGAKVVAVSDIKGGIYSENGFKFDDLLAWSKKIGSVVGFPGSKPITNEELLESDVDVLIPAAIEEQITEKNADKIKAKIILELANGPTTPEADEILYKKGKLVLPDVLSNSGGVIVSYFEWVQNNYGEYWTTEEVYDKLDQKITKAARQVLDTMDKYHVDPRTAAYVIAVKKVADAMKVRGWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AQ4</accession>
    <name>Q97AQ4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Succinate dehydrogenase hydrophobic membrane anchor subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0760262</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917002.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AQ4"/>
    <dbReference type="STRING" id="273116.gene:9381545"/>
    <dbReference type="PaxDb" id="273116-14324971"/>
    <dbReference type="GeneID" id="1441850"/>
    <dbReference type="KEGG" id="tvo:TVG0760262"/>
    <dbReference type="eggNOG" id="arCOG04162">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010222_0_0_2"/>
    <dbReference type="OrthoDB" id="56467at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03500">
        <property type="entry name" value="SQR_TypeA_SdhD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13815" checksum="B0A7588A5A2A2189" modified="2001-10-01" version="1">MEKSSKVRYGALNRFVQAATGLFLVFFLGVHLYVAHINFGNPVALFGSVVNQLHNPWWLAFFLIFVYIVTYHGINGLSHVIDDTSISDKSKKYIGIALMVIYVITIIYGTILAVLVSRMTFPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AS3</accession>
    <name>Q97AS3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Integrase / recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0741039</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00589">
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
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    <sequence length="123" mass="14493" checksum="899C26639C152E66" modified="2001-10-01" version="1">MFNCVLRLKEACDLKISNIYDDTIKVLGKGQKERLVYLPPETKEALMDYLKVRTPEKNLEDRDYVFTTASWKKLSYNYFTKICHEISIMAGVKWHPHMVRHTYATELFKSGVNIYYVARLLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
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    <name>Q97AT0_THEVO</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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            <dbReference type="EMBL" id="BAB59871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="69" mass="8075" checksum="5018925AAB1C560E" modified="2001-10-01" version="1">MFDGELHVGIRRKKSDIINIKKMITVIVKKRHNFVNKVPIYDDLLLNASLFTSKYNINGKLFNISRQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AT4</accession>
    <name>Q97AT4_THEVO</name>
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        <submittedName>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="GeneID" id="24779923"/>
    <dbReference type="KEGG" id="tvo:TVG0732301"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="103" mass="11819" checksum="4287B19639E50978" modified="2001-10-01" version="1">MNFSRYVNSVSYVYGALPVMVYKAENFGYFVPFALFLIFKVRQKTDFPFFSTLLYGSIQVGPVNYINSHEIVFAFHTYGSLLNLTVQVSLFIAVWASIIFQFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AT6</accession>
    <name>Q97AT6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0731305</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AT6"/>
    <dbReference type="STRING" id="273116.gene:9381512"/>
    <dbReference type="PaxDb" id="273116-14324939"/>
    <dbReference type="GeneID" id="1441827"/>
    <dbReference type="KEGG" id="tvo:TVG0731305"/>
    <dbReference type="eggNOG" id="arCOG00135">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923241_0_0_2"/>
    <dbReference type="OrthoDB" id="385715at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43266">
        <property type="entry name" value="MACROLIDE-EFFLUX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43266:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="33121" checksum="A84F4CD2066B2490" modified="2001-10-01" version="1">MIVLRQYIVTAVSIFGQTLKTDSFSAIIKKFLKEESYKKAVSLNFLTGSVTSLTGAIIGGVFLIYFSDYFVDVMISAVLISLLSAIPVIQKTQRVNENRNTFSGEMKSVGLFLRRISGFLLLAFFLNGLFISIDTYSSGLFNLVLKANPAYYTAFNMAVPIGMMVGTPLVNVRYFKMEEPAFISGMIFIFAPLLIILSLSKFPTLDIIDAFTIGLLLPLINIPLNSRLIKIIPGKIYGKISAVMRIFVQGAQPAMGALFNVLAIGISVKTVFLYVGLLVIPLALYGMSIVPRFFVSYFSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AU6</accession>
    <name>Q97AU6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Succinate dehydrogenase cytochrome b552 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0721034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AU6"/>
    <dbReference type="STRING" id="273116.gene:9381502"/>
    <dbReference type="PaxDb" id="273116-14324929"/>
    <dbReference type="KEGG" id="tvo:TVG0721034"/>
    <dbReference type="HOGENOM" id="CLU_2103651_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="115" mass="13230" checksum="FB74EB0974B80217" modified="2001-10-01" version="1">MNSIFPHSALYFNFCLKSYQETPVMPFATCSFRIIPSMFKSSTTTVYANLVIAVDTLCVKSFLVSLSFWYSSSILAICFLLFFTVFFLFSEFLLCPFNSLLLSLYAYRLFDAYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AV8</accession>
    <name>Q97AV8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0708014 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0708014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AV8"/>
    <dbReference type="PaxDb" id="273116-14324917"/>
    <dbReference type="KEGG" id="tvo:TVG0708014"/>
    <dbReference type="HOGENOM" id="CLU_867729_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="320" mass="37543" checksum="981418352E9C9A11" modified="2001-10-01" version="1">MDKPGKVKGRILIELAQALDDNPEPMSREELRERTGLSREDLKYHLKDSRTGLIPNGIVKEAQGKFSISFKNAKSVEKAVNYLCTRNKIRESIEQFFAESFMSVFGEVYHCDMHDLEQLSPDEFEDYNRFMEKSRAIMDEWKRPDIDSKALHLAKKLLRIRRGDKTPGFMFIYWRLAFHFYALAYYGNTEKMNAESLSLAGMPDFMMYDFANKMLKEDKSFFDIELRQALRRVFLADSYWISPGVKVIHSFGAFRDISFTEQVMFRYLQVDLQLRSLPITELDKTQTDIFKALKGKVPLGYEVIHEDNREIITRWTGKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AY2</accession>
    <name>Q97AY2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0678044 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0678044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760316.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AY2"/>
    <dbReference type="PaxDb" id="273116-14324893"/>
    <dbReference type="GeneID" id="1441784"/>
    <dbReference type="KEGG" id="tvo:TVG0678044"/>
    <dbReference type="eggNOG" id="arCOG01241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051789_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00397">
        <property type="entry name" value="DNA_BRE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="433" mass="49873" checksum="960FEB5306DA9E0A" modified="2001-10-01" version="1">MGTKKYEDFEKNERVNRWYLNVRARSPISADIWRRSLLRYCTINNTTPEGLLDMAQKGTLKDNFQDFTIRMIAEGRRGAYLAKVKEILHSWIRFNDIDYKIRINIPNERLNETTMDERVPTKEELSKILRMGTVRSRVSVSLMAFSGLRPEVLGNYEGTDGLTLGDIEDLDIDTLTFKNVPAKINVRNGLSKTRLKYFTFLGQEGCGYLKDYLDHRKASGEILKKESALLAPDPGNVQTAHGFLRTALIAREIRKAIRNSNLSMRPYVLRVYFATALDIAESKGSISHPWRQYIMGHKGDIEATYSTNKRLLPETIEGMRSAYLKCTKFFETEEKGIKEEDYQKMLRDSAIDTLTGAFGITLTDDQKEELRNLDTAEYQKRLGEIFKDRKADLLNNGNSQKVIPFKDVEKYIEQGWEYVRDFPGNKAIVRLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AZ2</accession>
    <name>Q97AZ2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transketolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0662032</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916926.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AZ2"/>
    <dbReference type="STRING" id="273116.gene:9381455"/>
    <dbReference type="PaxDb" id="273116-14324883"/>
    <dbReference type="GeneID" id="1441774"/>
    <dbReference type="KEGG" id="tvo:TVG0662032"/>
    <dbReference type="eggNOG" id="arCOG01053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_4_1_2"/>
    <dbReference type="OrthoDB" id="25494at2157"/>
    <dbReference type="PhylomeDB" id="Q97AZ2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47514">
        <property type="entry name" value="TRANSKETOLASE N-TERMINAL SECTION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47514:SF1">
        <property type="entry name" value="TRANSKETOLASE N-TERMINAL SECTION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase N-terminal" evidence="4">
        <location>
            <begin position="14"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="273" mass="30255" checksum="8490EE32E28ACDD4" modified="2001-10-01" version="1">MEATQIDLSDIALKIRREIIRMVYNAQSGHPGGSLSVTDILTVLYFKEMNIDPNKPKDPKRDRLILSKGHASPALYATLAIRGFFPVSDLSGFRKLDSHLEGHVTREVPGVEVSTGSLGQGLGIGVGMALAGRLDGMQYRVYVILGDGEMEEGNVWESLMAGYKYNLNNITAILDKNGIQLDGYTKDIMPMHDIKSMVESFGWNVIEIDGHNYDQIHEAVQASKNSDRPTFIIANTVKGKGVDFMENNPKYHGSPPESKELFERAMKQLGEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B37</accession>
    <name>Q97B37_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytosine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0613788</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916881.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B37"/>
    <dbReference type="STRING" id="273116.gene:9381410"/>
    <dbReference type="PaxDb" id="273116-14324838"/>
    <dbReference type="GeneID" id="1441729"/>
    <dbReference type="KEGG" id="tvo:TVG0613788"/>
    <dbReference type="eggNOG" id="arCOG03447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026016_3_0_2"/>
    <dbReference type="OrthoDB" id="27121at2157"/>
    <dbReference type="PhylomeDB" id="Q97B37"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF1">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="445" mass="48946" checksum="2F3DA2261FCA6804" modified="2001-10-01" version="1">MVTEHKFGDIETKNRIIKGALSDRIRDNPSSMFYIWFASNLTVGDFALGFIPVYLGLSESYAILSMVLGTLAGGILLAYMSMLGSINHMPQMEMGKGPFGRIGGSFLALLQWGNTAGWLTVNVILSSFALYEVLKIPYYFIILLIAVIVALTAALGYRAIRALERYMSYVLGILFIVIIIFMVLRHYTLIYSGSSSVSFIPAFGITFASAFSYIMSWGPYASDYSRHQYSPKSSRKVFLFTLGGSALATVFAEIVGMFVAIGSGNPSGNPATDLELVMGSFALAGMISLFLGGISANAINLYSNSLSFIAMGFKTRRITGVIIATLFSVILGIFGFTRFYGFYETFLFVLDYWITPWIGVMIAHFFLVHPNTSTMNNTKLNMAGVLSYLISILISIPFMSPAGLFNMPIASILGGVDISYFVSFFTSMILYVYLYGKLENRKLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B43</accession>
    <name>Q97B43_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Metalloprotease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0609642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916874.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B43"/>
    <dbReference type="STRING" id="273116.gene:9381404"/>
    <dbReference type="PaxDb" id="273116-14324832"/>
    <dbReference type="GeneID" id="1441722"/>
    <dbReference type="KEGG" id="tvo:TVG0609642"/>
    <dbReference type="eggNOG" id="arCOG00614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099718_0_0_2"/>
    <dbReference type="OrthoDB" id="86131at2157"/>
    <dbReference type="PhylomeDB" id="Q97B43"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052348">
        <property type="entry name" value="Metallopeptidase_M50B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864">
        <property type="entry name" value="ZINC METALLOPROTEASE MJ0611-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864:SF1">
        <property type="entry name" value="ZINC METALLOPROTEASE YWHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21215" checksum="3357BBED2C9559C3" modified="2001-10-01" version="1">MYGHNLNNEEKDILVSVLALTAAFGIAFRFRSPYGPIFVLAMAFLVSVTAFLMHELAHRYVARSYGGIAYFKMWPLGLLLALLTSVFGFIFAAPGAVNIGGLYRKDQIGKTALAGPAMNIFLGILFYGLSHITVLPVVALIFGYVAAMNFWFGFFNLLPIPPLDGYKVMNWDIYVFLVSIIIALIFVALVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B63</accession>
    <name>Q97B63_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycolate oxidase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0584082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B63"/>
    <dbReference type="STRING" id="273116.gene:9381383"/>
    <dbReference type="PaxDb" id="273116-14324810"/>
    <dbReference type="KEGG" id="tvo:TVG0584082"/>
    <dbReference type="eggNOG" id="arCOG00333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_2_1_2"/>
    <dbReference type="PhylomeDB" id="Q97B63"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF2">
        <property type="entry name" value="HETERODISULFIDE REDUCTASE RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="51"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="381" mass="43083" checksum="E10592A5B6818F58" modified="2001-10-01" version="1">MNEMELLIQEAGKCVNCGFCESVCPTLPASSFIASIGARGRVDIAKFVYKNGTVIDPSTSFYSCVDCYACVAVCPAGVNAGKVSEIGRQIVAKSGNAPEVAKMIVQITMLYKNPLAVREMCAKWATGIDFDKSSTILYTGNMYQLMAYLKSFSRVRKMIGSYDAKFARLIRERPSLIRALSRMYDRKMMDQMSIYLRNIVNALKKAGIKFGYLGEEEPYPGTFIYDLGFMDDFVSYAQYVSNLFREHGVERIITVDPHTYELLKYIYPRYVKFDFDVVYYLELIEPKKLEGRYAVHTPCHFSRYFDFTSFISKFIDVSPDTGQTKCCGGPDELLFPQISENISQRRFSELKAEGLPIITACPICYANLAKDDTVYDISALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B91</accession>
    <name>Q97B91_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum cofactor biosynthesis protein moeB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0555211</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B91"/>
    <dbReference type="STRING" id="273116.gene:9381352"/>
    <dbReference type="PaxDb" id="273116-14324781"/>
    <dbReference type="DNASU" id="1441083"/>
    <dbReference type="KEGG" id="tvo:TVG0555211"/>
    <dbReference type="eggNOG" id="arCOG00217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_3_0_2"/>
    <dbReference type="PhylomeDB" id="Q97B91"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045515">
        <property type="entry name" value="Glp_gephyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011068">
        <property type="entry name" value="PRK14498.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF5">
        <property type="entry name" value="GEPHYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12727">
        <property type="entry name" value="PBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="3">
        <location>
            <begin position="189"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="629" mass="68859" checksum="B7B84483BA548044" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-12-06" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B95</accession>
    <name>RS4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps4</name>
        <name type="ordered locus">TV0562</name>
        <name type="ORF">TVG0550709</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916821.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B95"/>
    <dbReference type="SMR" id="Q97B95"/>
    <dbReference type="STRING" id="273116.gene:9381347"/>
    <dbReference type="PaxDb" id="273116-14324777"/>
    <dbReference type="GeneID" id="1441079"/>
    <dbReference type="KEGG" id="tvo:TVG0550709"/>
    <dbReference type="eggNOG" id="arCOG04239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089738_1_1_2"/>
    <dbReference type="OrthoDB" id="10429at2157"/>
    <dbReference type="PhylomeDB" id="Q97B95"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1050.10">
        <property type="entry name" value="Ribosomal Protein S4 Delta 41, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_A">
        <property type="entry name" value="Ribosomal_uS4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022802">
        <property type="entry name" value="Ribosomal_uS4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000132524" description="Small ribosomal subunit protein uS4">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="106"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="200" mass="23161" checksum="71D8DE99D5F122F7" modified="2001-10-01" version="1">MGDPKFHHKKYSTPRHPWEKDRIDEENKILVKYGLKNKREIWRSEAMLSSIRSQARYLRARLRASDANAQKQLERMIKRLSRYKILSDKATLDDVLSLTVENILDRRLQTIVFKKNLALSEKQARQLITHGHITVNGRRVTVPGMLVEAQYEDTIAYYENSPIANELHPIRQALLSPAERVKEEAEKEAAASEDGGEQDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-12" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B98</accession>
    <name>FEN_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flap endonuclease 1</fullName>
            <shortName evidence="2">FEN-1</shortName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fen</name>
        <name type="ordered locus">TV0559</name>
        <name type="ORF">TVG0548166</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916818.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B98"/>
    <dbReference type="SMR" id="Q97B98"/>
    <dbReference type="STRING" id="273116.gene:9381344"/>
    <dbReference type="PaxDb" id="273116-14324774"/>
    <dbReference type="GeneID" id="1441076"/>
    <dbReference type="KEGG" id="tvo:TVG0548166"/>
    <dbReference type="eggNOG" id="arCOG04050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032444_0_0_2"/>
    <dbReference type="OrthoDB" id="9593at2157"/>
    <dbReference type="PhylomeDB" id="Q97B98"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09903">
        <property type="entry name" value="H3TH_FEN1-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000016">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019973">
        <property type="entry name" value="Flap_endonuc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03674">
        <property type="entry name" value="fen_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000154068" description="Flap endonuclease 1">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-domain">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="I-domain">
        <location>
            <begin position="115"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00614"/>
        </source>
    </evidence>
    <sequence length="335" mass="38264" checksum="8544CDF9E61EAF38" modified="2001-10-01" version="1">MGTDISDIVVSHQTSLKDQANQVFAIDTYNILYQLLSNVRQYDGTPLMDSSGNVTSHLYGIFYRTVNLVENGIKPIFVFDGKPSPLKNRTLEIRQLAKEKAKAELEEAISRGEENLKQYYSRINYITPQIVNDTKELLTYMGIPYVDAPSEGEAQASYMTRKDADGVISQDYDCLLFGAKKILRNFAIYGRRKVPRKNVYRTVYPEYVMLDEVLKKNGINQDQLIEIGILVGTDFNEGIKGIGAKKALALIKKEGNIKAVLNKIGKNIENLDEIIDFFKNPPVVDVKYVFGKPDPKKIEEFLCVVHDFSRDRILEHINTYVKYYNKSVQFRLDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BD2</accession>
    <name>Q97BD2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0516404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916782.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BD2"/>
    <dbReference type="STRING" id="273116.gene:9381308"/>
    <dbReference type="PaxDb" id="273116-14324739"/>
    <dbReference type="GeneID" id="1441040"/>
    <dbReference type="KEGG" id="tvo:TVG0516404"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025894_3_0_2"/>
    <dbReference type="OrthoDB" id="56622at2157"/>
    <dbReference type="PhylomeDB" id="Q97BD2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="399" mass="43021" checksum="8B7FD23053032725" modified="2001-10-01" version="1">MDTGNTSDDAGYARSFRLILTSRSARSIGIIFVALSLPLYLLILHVSIVNIGIIYVGTALVNVSFSLGIGMLGDRIGYKYSLMLGELPALVATTFLAFSKSVDLIVIATIVSGSVGASGGMRGSFSVGITPYIARIWKDQSERISKMAYITFVASISSIGGSLMLYSHAYVSKYFGNAGAFELLYKLSFLLILVSFLSLSLIKYDGKQNKTTKFLRRETLKFSARVITTNIINGSAIGFSMALITAWFEIRYGASPSRIGLIFTISYITTALGSYVAGHIKIERKSAVYLGSATRIAQGLLLIAMALSPLFYLASAIYIVRTIVAGFGTPIRNVVNIQGVREEDLGTASSIQGAPSRIAQSTSGIAGFLMDYYPPLPVEIGGIIQAVGGYVYYRLLKKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BE8</accession>
    <name>THIE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-phosphate synthase</fullName>
            <shortName evidence="1">TP synthase</shortName>
            <shortName evidence="1">TPS</shortName>
            <ecNumber evidence="1">2.5.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Thiamine-phosphate pyrophosphorylase</fullName>
            <shortName evidence="1">TMP pyrophosphorylase</shortName>
            <shortName evidence="1">TMP-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiE</name>
        <name type="ordered locus">TV0508</name>
        <name type="ORF">TVG0496174</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiamine-phosphate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.3" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916766.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BE8"/>
    <dbReference type="SMR" id="Q97BE8"/>
    <dbReference type="STRING" id="273116.gene:9381291"/>
    <dbReference type="PaxDb" id="273116-14324723"/>
    <dbReference type="GeneID" id="1441024"/>
    <dbReference type="KEGG" id="tvo:TVG0496174"/>
    <dbReference type="eggNOG" id="arCOG01089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018272_3_2_2"/>
    <dbReference type="OrthoDB" id="85572at2157"/>
    <dbReference type="PhylomeDB" id="Q97BE8"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00141"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000096">
        <property type="entry name" value="Thiamine-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00097">
        <property type="entry name" value="TMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034291">
        <property type="entry name" value="TMP_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00693">
        <property type="entry name" value="thiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20857:SF23">
        <property type="entry name" value="THIAMINE BIOSYNTHETIC BIFUNCTIONAL ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20857">
        <property type="entry name" value="THIAMINE-PHOSPHATE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="Thiamin phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000157077" description="Thiamine-phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="37"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="183"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00097"/>
        </source>
    </evidence>
    <sequence length="211" mass="23300" checksum="0A05D787127C5D72" modified="2001-10-01" version="1">MNEKKLRGLYLVTYDYYGAEFYEKIIDAIEGGVDILQYRDKTNAYSVKLQVARKLTSIAADYGIPFFVDDDPKLAKEAGADGVHIGRDDPPIEQVRDVFNGLIGVSTYGNLELATEAEKKGASYVAFGSFFHTDTKKDAGIYDIKILEKARHFVKIPIFVIGGINLETITKFCGYSIDGIAVVSAILAAENVKKAAKELKEKVEDIINGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BH8</accession>
    <name>Q97BH8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nmd3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0465345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916736.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BH8"/>
    <dbReference type="STRING" id="273116.gene:9381259"/>
    <dbReference type="PaxDb" id="273116-14324692"/>
    <dbReference type="DNASU" id="1440994"/>
    <dbReference type="GeneID" id="1440994"/>
    <dbReference type="KEGG" id="tvo:TVG0465345"/>
    <dbReference type="eggNOG" id="arCOG04149">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065087_0_0_2"/>
    <dbReference type="OrthoDB" id="15051at2157"/>
    <dbReference type="PhylomeDB" id="Q97BH8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="335" mass="38154" checksum="0A896F0A760069B8" modified="2001-10-01" version="1">MKCVVCGRNEAVEHGMCSSCIYDNTNVENIGPVSLVFCPKCGSIRIGKKWYRDSYGNKLLPMLLDEVKCSDVNARISGDEKGIFFDEKNKQIILPVTIKRSNMKDKIQNVAIPYSVTYISCPTCNKITGSYFEATIQLRTVSTKYDNILESVLESIEAIVEKRKKEDAESFISKVEKKPEGIDIYLGKRTDGDVVSSFVLDHFFSSIIVTKTLAGVKDGKKFFRFTYSLRILDAKEGSLLSVNRKKYILTAIRNRHIDVIDVETEDKTRINRNTFFVNDGKIVTKEPQMRKFIVVSRQGNESLLMDAQTFKMITVKGTPESDEVYLFTYKGKYFM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-02-10" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BJ3</accession>
    <name>PELO_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein pelota homolog</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pelA</name>
        <name type="ordered locus">TV0462</name>
        <name type="ORF">TVG0451753</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain has the RNA-binding Sm fold. It harbors the endoribonuclease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916721.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BJ3"/>
    <dbReference type="SMR" id="Q97BJ3"/>
    <dbReference type="STRING" id="273116.gene:9381243"/>
    <dbReference type="PaxDb" id="273116-14324677"/>
    <dbReference type="GeneID" id="1440979"/>
    <dbReference type="KEGG" id="tvo:TVG0451753"/>
    <dbReference type="eggNOG" id="arCOG01741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023334_0_0_2"/>
    <dbReference type="OrthoDB" id="31300at2157"/>
    <dbReference type="PhylomeDB" id="Q97BJ3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01853">
        <property type="entry name" value="PelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023521">
        <property type="entry name" value="Pelota_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000361826" description="Protein pelota homolog">
        <location>
            <begin position="1"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01853"/>
        </source>
    </evidence>
    <sequence length="340" mass="38573" checksum="6215A52DD90D9E99" modified="2001-10-01" version="1">MRLLDKDEKLGRYTLRIENLDDLWYLKNIIAPGDKISAVTFRRIEESSDMQRSREKERIPIRVTIEVEKIEFQDFENRLRILGKVTNGPEDTIGKYQSITVSEETELDVIKVWDDDSINMLKEATEQEHAAVYLAIALDDDEANVFIIHPYGIQQMGTIYSGRSGKYSESSYSETSYFSAIENAIKQFKYPIIVIGPGFAKNGLSAFLKERNYKVVAVSSSNRTDQGAIYEFISSEDGKKLISKEQISRDNEIVSAFLSSLRRGSGIYGSEEIRKYLEMGALSDLIITDKKFRSETGRELLSLARSVGTDIHIVSTSNDMGEIIEKFGGYAGILRYKVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BJ6</accession>
    <name>Q97BJ6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0449738 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0449738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916718.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BJ6"/>
    <dbReference type="STRING" id="273116.gene:9381240"/>
    <dbReference type="PaxDb" id="273116-14324674"/>
    <dbReference type="GeneID" id="1440976"/>
    <dbReference type="KEGG" id="tvo:TVG0449738"/>
    <dbReference type="eggNOG" id="arCOG05327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1478855_0_0_2"/>
    <dbReference type="OrthoDB" id="56638at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="181" mass="19765" checksum="1DBC1E89473B36D4" modified="2001-10-01" version="1">MKALAILSVFIALIFVALPAVSSASTPTLPPPPSSLPSITYYYDSTHYTITSTEWGSFFGEIIDNKSYVSYDWINVSTQDLIVFDLSYTGLNPFGLELVVALSSIGFPSVSNMTKAFLSIENKPSQIPGYSNIQALDAGAYPGFSFSKPVTNYTGEYIGIGIIVALFASIFVLYYVFNRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BK3</accession>
    <name>Q97BK3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NDP-sugar epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0440460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="3">
        <citation type="journal article" date="2012" name="J. Biol. Chem." volume="287" first="12966" last="12974">
            <title>Crystal structure of binary and ternary complexes of archaeal UDP-galactose 4-epimerase-like L-threonine dehydrogenase from Thermoplasma volcanium.</title>
            <authorList>
                <person name="Yoneda K."/>
                <person name="Sakuraba H."/>
                <person name="Araki T."/>
                <person name="Ohshima T."/>
            </authorList>
            <dbReference type="PubMed" id="22374996"/>
            <dbReference type="DOI" id="10.1074/jbc.m111.336958"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS) IN COMPLEX WITH NAD(+) AND PYRUVATE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916712.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="3A1N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.07 A"/>
        <property type="chains" value="A/B=1-317"/>
    </dbReference>
    <dbReference type="PDB" id="3A4V">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.78 A"/>
        <property type="chains" value="A/B=1-317"/>
    </dbReference>
    <dbReference type="PDB" id="3A9W">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A/B=1-317"/>
    </dbReference>
    <dbReference type="PDB" id="3AJR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.77 A"/>
        <property type="chains" value="A/B=1-317"/>
    </dbReference>
    <dbReference type="PDBsum" id="3A1N"/>
    <dbReference type="PDBsum" id="3A4V"/>
    <dbReference type="PDBsum" id="3A9W"/>
    <dbReference type="PDBsum" id="3AJR"/>
    <dbReference type="AlphaFoldDB" id="Q97BK3"/>
    <dbReference type="SMR" id="Q97BK3"/>
    <dbReference type="STRING" id="273116.gene:9381233"/>
    <dbReference type="PaxDb" id="273116-14324667"/>
    <dbReference type="GeneID" id="1440970"/>
    <dbReference type="KEGG" id="tvo:TVG0440460"/>
    <dbReference type="eggNOG" id="arCOG04468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_19_1_2"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="PhylomeDB" id="Q97BK3"/>
    <dbReference type="EvolutionaryTrace" id="Q97BK3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008743">
        <property type="term" value="F:L-threonine 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05272">
        <property type="entry name" value="TDH_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051225">
        <property type="entry name" value="NAD(P)_epim/dehydratase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42687">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42687:SF1">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5 6 7" id="KW-0002">3D-structure</keyword>
    <keyword evidence="5 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 6 7">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6 7">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6 7">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6 7">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6 7">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6 7">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6 7">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6 7">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7 8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3A1N"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3A4V"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="3A9W"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="3AJR"/>
        </source>
    </evidence>
    <sequence length="317" mass="35843" checksum="0F0A302A440523AE" modified="2001-10-01" version="1">MILVTGSSGQIGTELVPYLAEKYGKKNVIASDIVQRDTGGIKFITLDVSNRDEIDRAVEKYSIDAIFHLAGILSAKGEKDPALAYKVNMNGTYNILEAAKQHRVEKVVIPSTIGVFGPETPKNKVPSITITRPRTMYGVTKIAAELLGQYYYEKFGLDVRSLRYPGIISYKAEPTAGTTDYAVEIFYYAVKREKYKCYLAPNRALPMMYMPDALKALVDLYEADRDKLVLRNGYNVTAYTFTPSELYSKIKERIPEFEIEYKEDFRDKIAATWPESLDSSEASNEWGFSIEYDLDRTIDDMIDHISEKLGIEGKHAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BK6</accession>
    <name>RL24E_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L24e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl24e</name>
        <name type="ordered locus">TV0449</name>
        <name type="ORF">TVG0437979</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BK6"/>
    <dbReference type="SMR" id="Q97BK6"/>
    <dbReference type="STRING" id="273116.gene:9381230"/>
    <dbReference type="PaxDb" id="273116-14324664"/>
    <dbReference type="KEGG" id="tvo:TVG0437979"/>
    <dbReference type="eggNOG" id="arCOG01950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190191_0_0_2"/>
    <dbReference type="OrthoDB" id="55506at2157"/>
    <dbReference type="PhylomeDB" id="Q97BK6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_eL24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055345">
        <property type="entry name" value="Ribosomal_eL24-rel_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF034186">
        <property type="entry name" value="PRK14891.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000136931" description="Large ribosomal subunit protein eL24">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="66" mass="7766" checksum="964CD18135AA9C26" modified="2001-10-01" version="1">MLARTCSFCGKTIEPGTGIMYVRKDGAILYFCSNKCKKNMIGLNRVPRYVRWTNEYHELKKMRTRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-15" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BK8</accession>
    <name>RL7A_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L7Ae</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein L8e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl7ae</name>
        <name type="ordered locus">TV0447</name>
        <name type="ORF">TVG0437556</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Probably part of the RNase P complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916707.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BK8"/>
    <dbReference type="SMR" id="Q97BK8"/>
    <dbReference type="STRING" id="273116.gene:9381228"/>
    <dbReference type="PaxDb" id="273116-14324662"/>
    <dbReference type="GeneID" id="1440965"/>
    <dbReference type="KEGG" id="tvo:TVG0437556"/>
    <dbReference type="eggNOG" id="arCOG01751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084513_4_0_2"/>
    <dbReference type="OrthoDB" id="25810at2157"/>
    <dbReference type="PhylomeDB" id="Q97BK8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000020">
        <property type="entry name" value="50S ribosomal protein L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00326">
        <property type="entry name" value="Ribosomal_eL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022481">
        <property type="entry name" value="Ribosomal_eL8_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03677">
        <property type="entry name" value="eL8_ribo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000136809" description="Large ribosomal subunit protein eL8">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="121" mass="13389" checksum="19FE3A349D3F9D36" modified="2001-10-01" version="1">MEKSYVKFETPEDVSQKALDLVESAFRSGKIKKGTNEVIKSIERGESKLVVIAEDVNPPEVVYYLPSLCEDKKVPYVYVKKKADLGSKVGIASAASVSIVDYGKNEELYKSIVSAVEQLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BM2</accession>
    <name>Q97BM2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0422635 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0422635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916691.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BM2"/>
    <dbReference type="STRING" id="273116.gene:9381212"/>
    <dbReference type="PaxDb" id="273116-14324648"/>
    <dbReference type="GeneID" id="1440949"/>
    <dbReference type="KEGG" id="tvo:TVG0422635"/>
    <dbReference type="eggNOG" id="arCOG03681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010200_0_0_2"/>
    <dbReference type="OrthoDB" id="12193at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="121" mass="13834" checksum="114D1D278BF3C9DF" modified="2001-10-01" version="1">MGYEEGEFWHGIALKVLYEGSKTVYKKDPVYGNVPAIERSAVFRRSIGEPKGQIADWRCTFPGDPRSVHAVEYIDRYEIHVDKFDPSKHPLKHVVYDAPSVGLFILPLTIIAALFLARKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BN5</accession>
    <name>Q97BN5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">TVG0406934</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BN5"/>
    <dbReference type="STRING" id="273116.gene:9381198"/>
    <dbReference type="PeroxiBase" id="4360">
        <property type="entry name" value="TvoBCP"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324635"/>
    <dbReference type="KEGG" id="tvo:TVG0406934"/>
    <dbReference type="eggNOG" id="arCOG00310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_2_2"/>
    <dbReference type="PhylomeDB" id="Q97BN5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000007">
        <property type="entry name" value="Thioredoxin-dependent thiol peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006960">
        <property type="entry name" value="PRK09437.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF4">
        <property type="entry name" value="AHPC_TSA FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="6"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="11">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAB59562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="154" mass="17353" checksum="25D6EA2AECB882D9" modified="2001-10-01" version="1">MYMELLKVGDQAPDFETVDQSGSKVRLSDFKGQPVVLYFYPKDNTPGCTTEACNFRDNFNSFKEAGIKVIGVSVDSPESHKRFAEKYQLNFTLASDKEKEICKKYGVLGLATAKRVTYIIDRDGKIVYVYPKVTPKDHAVEVMNKIKELGLIPS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-11" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BN7</accession>
    <name>SPEE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="1">PAPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Spermidine synthase</fullName>
            <shortName evidence="1">SPDS</shortName>
            <shortName evidence="1">SPDSY</shortName>
            <ecNumber evidence="1">2.5.1.16</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">speE</name>
        <name type="ordered locus">TV0418</name>
        <name type="ORF">TVG0404401</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916675.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BN7"/>
    <dbReference type="SMR" id="Q97BN7"/>
    <dbReference type="STRING" id="273116.gene:9381196"/>
    <dbReference type="PaxDb" id="273116-14324633"/>
    <dbReference type="GeneID" id="1440933"/>
    <dbReference type="KEGG" id="tvo:TVG0404401"/>
    <dbReference type="eggNOG" id="arCOG00050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048199_0_0_2"/>
    <dbReference type="OrthoDB" id="10538at2157"/>
    <dbReference type="PhylomeDB" id="Q97BN7"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156537" description="Polyamine aminopropyltransferase">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="PABS" evidence="1">
        <location>
            <begin position="5"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="140"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="158"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
        </source>
    </evidence>
    <sequence length="273" mass="30970" checksum="11413231117C25C9" modified="2001-10-01" version="1">MKLIENWFSERYSDNLQLSFRVSDQLLSIKTDYQRIDLFDTYDFGKLLAIDGTVQLTERDEYIYHELITMIPYHMTKNPPQSALVIGGGDGGAARRLLDLGLNKIVNVEIDGQVVEVSKRFFPDLSSAFTDKRVNLIIDDGIKYVRKCNEKFDLVIIDSTDPEGPAEGLFSMEFYSDITKILSDGGVIVSQSGSPFYQPKALKLAYTGMRRLFKDVKVYTGFIPTYPSGFWSFTIASEDAMNPRKPAIKGKYFNEDVLDGVFKLPQFVKDLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BP0</accession>
    <name>Q97BP0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl44e</name>
        <name evidence="5" type="ORF">TVG0402431</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916672.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BP0"/>
    <dbReference type="STRING" id="273116.gene:9381193"/>
    <dbReference type="PaxDb" id="273116-14324630"/>
    <dbReference type="GeneID" id="1440930"/>
    <dbReference type="KEGG" id="tvo:TVG0402431"/>
    <dbReference type="eggNOG" id="arCOG04109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114645_3_0_2"/>
    <dbReference type="OrthoDB" id="52456at2157"/>
    <dbReference type="PhylomeDB" id="Q97BP0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004425">
        <property type="entry name" value="PRK05767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="93" mass="10843" checksum="D6C9DD4F6FA7EA05" modified="2001-10-01" version="1">MKMPKKIVTYCPYCKKHTTHSVERVRKRKASELKAGQRRFRRVTSGYGGFPRPRFEGREKPTKRIALRLVCDECHKAITPPGIRAKKFEIVEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-10-24" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BP8</accession>
    <name>SPEH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <shortName evidence="1">AdoMetDC</shortName>
            <shortName evidence="1">SAMDC</shortName>
            <ecNumber evidence="1">4.1.1.50</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="1">S-adenosylmethionine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">S-adenosylmethionine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">speH</name>
        <name type="ordered locus">TV0407</name>
        <name type="ORF">TVG0395474</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-adenosyl-L-methionine + H(+) = S-adenosyl 3-(methylsulfanyl)propylamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="1">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916663.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BP8"/>
    <dbReference type="SMR" id="Q97BP8"/>
    <dbReference type="STRING" id="273116.gene:9381185"/>
    <dbReference type="PaxDb" id="273116-14324622"/>
    <dbReference type="GeneID" id="1440921"/>
    <dbReference type="KEGG" id="tvo:TVG0395474"/>
    <dbReference type="eggNOG" id="arCOG00279">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125470_2_3_2"/>
    <dbReference type="OrthoDB" id="114016at2157"/>
    <dbReference type="PhylomeDB" id="Q97BP8"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.90.10">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00464">
        <property type="entry name" value="AdoMetDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017716">
        <property type="entry name" value="S-AdoMet_deCOase_pro-enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03330">
        <property type="entry name" value="SAM_DCase_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866:SF2">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_0000030159" description="S-adenosylmethionine decarboxylase beta chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030160" description="S-adenosylmethionine decarboxylase alpha chain" evidence="1">
        <location>
            <begin position="64"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="1">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="1">
        <location>
            <begin position="63"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00464"/>
        </source>
    </evidence>
    <sequence length="130" mass="14561" checksum="92E6060AF363D3C3" modified="2001-10-01" version="1" precursor="true">MKVGVGIHIIADFYGVDSKLISTTERMYPIIEGAVKYGRLTKISSDYYQFRPQGASGVVLLAESHLSFHTWPEYGLVTLDIYTCGDPKTADDAFEYLTRELKPTSVTTRKIVRGDLVGEEGLNEMQVEIH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BX7</accession>
    <name>RL3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl3</name>
        <name type="ordered locus">TV0328</name>
        <name type="ORF">TVG0333226</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L24e.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916582.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BX7"/>
    <dbReference type="SMR" id="Q97BX7"/>
    <dbReference type="STRING" id="273116.gene:9381105"/>
    <dbReference type="PaxDb" id="273116-14324543"/>
    <dbReference type="GeneID" id="1440840"/>
    <dbReference type="KEGG" id="tvo:TVG0333226"/>
    <dbReference type="eggNOG" id="arCOG04070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033361_2_0_2"/>
    <dbReference type="OrthoDB" id="6121at2157"/>
    <dbReference type="PhylomeDB" id="Q97BX7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_A">
        <property type="entry name" value="Ribosomal_uL3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019928">
        <property type="entry name" value="Ribosomal_uL3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03626">
        <property type="entry name" value="L3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003261">
        <property type="entry name" value="PRK04231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000077225" description="Large ribosomal subunit protein uL3">
        <location>
            <begin position="1"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="331" mass="37560" checksum="9D349B8AF5D4DBFA" modified="2001-10-01" version="1">MATPHHSRRGSMAYYPRVRAKSIEPRIRSWPEISGPVKVQGFAGFKVGMTHVEMVDYRKTSVTAGQPIFVPVTVIEVPPLDVIGIRLYDEDEEGNMVVVYEKWTQNLDKELFKKITTFKEVKEKPVPETYADVRLIVATRNKDVPGIPSKKPEIFELRIGGGNSVKERFEYATAHLGKTIRFEDFSKPGKFVDVLSVTKGKGFTGHVQRFGVKLLPRKNRKHRRMIGTLGPWHPDWVRNTVPQAGQMGYQQRTISNVRVLKYSKGEDADTINVRGGFLHYGLVKNDYVLLFGSVPGPAKRLIKMRDPARQKVPDIDNVKLDYISLESKQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BY6</accession>
    <name>Q97BY6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0325598 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0325598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BY6"/>
    <dbReference type="PaxDb" id="273116-14324534"/>
    <dbReference type="KEGG" id="tvo:TVG0325598"/>
    <dbReference type="HOGENOM" id="CLU_2839577_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="65" mass="7642" checksum="F1FB2598832B7252" modified="2001-10-01" version="1">MRMHSDHFLKIILKRSTTNTHGDLIIILNASLFQDLDCDKELKIILNFLTNNSKNMRYNDCSFLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BZ4</accession>
    <name>RRP42_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exosome complex component Rrp42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rrp42</name>
        <name type="ordered locus">TV0311</name>
        <name type="ORF">TVG0320734</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Contributes to the structuring of the Rrp41 active site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase PH family. Rrp42 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916566.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BZ4"/>
    <dbReference type="SMR" id="Q97BZ4"/>
    <dbReference type="STRING" id="273116.gene:9381086"/>
    <dbReference type="PaxDb" id="273116-14324526"/>
    <dbReference type="GeneID" id="1440824"/>
    <dbReference type="KEGG" id="tvo:TVG0320734"/>
    <dbReference type="eggNOG" id="arCOG01574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038194_0_0_2"/>
    <dbReference type="OrthoDB" id="30932at2157"/>
    <dbReference type="PhylomeDB" id="Q97BZ4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11365">
        <property type="entry name" value="RNase_PH_archRRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000017">
        <property type="entry name" value="Exosome complex component Rrp42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00622">
        <property type="entry name" value="Exosome_Rrp42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020869">
        <property type="entry name" value="Rrp42_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003282">
        <property type="entry name" value="PRK04282.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0271">Exosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140009" description="Exosome complex component Rrp42">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00622"/>
        </source>
    </evidence>
    <sequence length="260" mass="28502" checksum="D4FE28C302AD1D12" modified="2001-10-01" version="1">MVKESAEILSEIRKNYILTTMKGGKRIDGRLPDEFREITIIENYVPRANGSAYVALGKTRVVAGVKIEAGEPFPDTPDQGVLTTNVELLPIAFPSFEAGPPNDLAIEVSRVVDRGIRESKMISPDKLVIEQGKKVWIVFLDINVLDYDGNLIDACTIAAVSALRNAIVPASREGGEDFKLPVVNTPISVTMVKIGDTLVCDPSLEEDQICGGRITVTTTEDGHIRAMQKGEIGVFTLDDVKKAIKMSLKVGKNIREKYFR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BZ5</accession>
    <name>RRP41_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exosome complex component Rrp41</fullName>
            <ecNumber evidence="1">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rrp41</name>
        <name type="ordered locus">TV0310</name>
        <name type="ORF">TVG0319949</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic component of the exosome, which is a complex involved in RNA degradation. Has 3'-&gt;5' exoribonuclease activity. Can also synthesize heteromeric RNA-tails.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase PH family. Rrp41 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BZ5"/>
    <dbReference type="SMR" id="Q97BZ5"/>
    <dbReference type="STRING" id="273116.gene:9381085"/>
    <dbReference type="PaxDb" id="273116-14324525"/>
    <dbReference type="KEGG" id="tvo:TVG0319949"/>
    <dbReference type="eggNOG" id="arCOG01575">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063514_0_0_2"/>
    <dbReference type="OrthoDB" id="24266at2157"/>
    <dbReference type="PhylomeDB" id="Q97BZ5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11366">
        <property type="entry name" value="RNase_PH_archRRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000004">
        <property type="entry name" value="Exosome complex component Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00591">
        <property type="entry name" value="Exosome_Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011807">
        <property type="entry name" value="Rrp41"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02065">
        <property type="entry name" value="ECX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0271">Exosome</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000139994" description="Exosome complex component Rrp41">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00591"/>
        </source>
    </evidence>
    <sequence length="248" mass="27259" checksum="9785DE9D7BCCC9AC" modified="2001-10-01" version="1">MIKTETSKIKLINEDNLRLDGRSFNELRPIKIEAGVLNRADGSAYIEWGGNKIIVGVYGPKEAYPKHSQDIDHAVVKARYNMAAFSVDERKRPGPDRRTMEISKVISEALSSSIMIEQFPRAEIDVYIEVLQADAGTRIAGLTAATVALADAGIPMRDMVVGCTAGKVDGHIVLDLSKEEDNFGEADIPMAIMPKTGEIVLLQMDGDVTEDEFYEATSMIIEATKKISQIQRNALLNKYKIEGIEGGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BZ6</accession>
    <name>RRP4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exosome complex component Rrp4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rrp4</name>
        <name type="ordered locus">TV0309</name>
        <name type="ORF">TVG0319243</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Confers strong poly(A) specificity to the exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916564.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BZ6"/>
    <dbReference type="SMR" id="Q97BZ6"/>
    <dbReference type="STRING" id="273116.gene:9381084"/>
    <dbReference type="PaxDb" id="273116-14324524"/>
    <dbReference type="GeneID" id="1440822"/>
    <dbReference type="KEGG" id="tvo:TVG0319243"/>
    <dbReference type="eggNOG" id="arCOG00678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00623"/>
        </source>
    </evidence>
    <sequence length="236" mass="26388" checksum="CBD3944E840FB268" modified="2001-10-01" version="1">MYQLGDVKKIVLPGDPVEVQGKIRNGIYRGPDNKYYSEYFGTLQVSDQYVDVVPFSGQYIPRKGDKVIGKIIEVGPSTWTVDINSPYFAMLHMNDTPWRISSGDLKRYLNSGDYVYAKIMSVNEIKESWLTLKEPGLKKLEGGHMVLIHASRVPRVIGKGGGMVNMVKELTSTRIIIGQNGLIWIDGPIEGVTMAIAAIEMIEREAHTEGLTVRVESFLKELRGDKDGNQQDKANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C07</accession>
    <name>Q97C07_THEVO</name>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
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                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="interaction">
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    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C68</accession>
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        <lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <proteinExistence type="predicted"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="260" mass="29034" checksum="79251AE11F173D9B" modified="2001-10-01" version="1">MKVEQCPQCGTIIPAGSDVCPICGYTLKKKTTDNSWRGIFASIFARRRILTMVLVFIFEIALMFVITAIPMSFSQAKAIAGQTEPSLQAVRSLPILSRSVSIFSHNYEIASIEIVPVIGQIFFVISTYSTAIVLDALAIGQGVPGPIALFTLLLLPYSWLELPSYAIAATEGGFLIASIFRKQFRVEIKRAVIVWLLVGIELLTAGIFESWTIYLETSSNPFAIFITWIPYVIILAIFITVVRSFLKRYESSKSRERQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C69</accession>
    <name>Q97C69_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0245034 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0245034</name>
    </gene>
    <organism evidence="2 3">
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916489.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C69"/>
    <dbReference type="PaxDb" id="273116-14324449"/>
    <dbReference type="GeneID" id="1440749"/>
    <dbReference type="KEGG" id="tvo:TVG0245034"/>
    <dbReference type="eggNOG" id="arCOG03769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147147_0_0_2"/>
    <dbReference type="OrthoDB" id="57468at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="133" mass="14587" checksum="E5E38585DD87CEF8" modified="2001-10-01" version="1">MFIIIGDLIAFVISVLVTLIIASVPVWVAAKIVAKRSTFGQAVAATLLSLIIFFILLFVFSIFTPFIGGIFGFIGALWVFRKVYNIGWVDSLALTIIAIFIAIMIYIILGAFLFPVIHTSMIHEHAVVTSIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C70</accession>
    <name>Q97C70_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0244352 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0244352</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C70"/>
    <dbReference type="PaxDb" id="273116-14324448"/>
    <dbReference type="KEGG" id="tvo:TVG0244352"/>
    <dbReference type="HOGENOM" id="CLU_3021184_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="55" mass="6398" checksum="DF1CFECC52D6860D" modified="2001-10-01" version="1">MGSGLNAERPCLSKCKARHLYRIEYRYPAARLPADDQIAMTGNQRLIENFFIEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CD1</accession>
    <name>Q97CD1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Iron-binding zinc finger CDGSH type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0180289</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916426.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CD1"/>
    <dbReference type="STRING" id="273116.gene:9380941"/>
    <dbReference type="PaxDb" id="273116-14324385"/>
    <dbReference type="GeneID" id="1441656"/>
    <dbReference type="KEGG" id="tvo:TVG0180289"/>
    <dbReference type="eggNOG" id="arCOG01667">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145019_3_0_2"/>
    <dbReference type="OrthoDB" id="5781at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.90">
        <property type="entry name" value="CDGSH iron-sulfur domain, mitoNEET-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018967">
        <property type="entry name" value="FeS-contain_CDGSH-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042216">
        <property type="entry name" value="MitoNEET_CISD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09360">
        <property type="entry name" value="zf-CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00704">
        <property type="entry name" value="ZnF_CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron-binding zinc finger CDGSH type" evidence="5">
        <location>
            <begin position="10"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="69" mass="8041" checksum="C775E1EE9FAF4913" modified="2001-10-01" version="1">MARLVLHTRDHPYVVKVGDREVHICGCGLSSHKPYCDGTHKITTDEGEKIYIYDADKKREEFHSFYDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CE0</accession>
    <name>Q97CE0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0172799 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0172799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916417.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CE0"/>
    <dbReference type="STRING" id="273116.gene:9380932"/>
    <dbReference type="PaxDb" id="273116-14324376"/>
    <dbReference type="GeneID" id="1441647"/>
    <dbReference type="KEGG" id="tvo:TVG0172799"/>
    <dbReference type="eggNOG" id="arCOG03473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040014_0_0_2"/>
    <dbReference type="OrthoDB" id="136106at2157"/>
    <dbReference type="PhylomeDB" id="Q97CE0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34614">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34614:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="186"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="459" mass="54129" checksum="CA98845A4E466114" modified="2001-10-01" version="1">MDNIPDWVLRQKRKGIEIRRFGDRYYAYEVTSKYDKELKRARKVTGKYLGVVTKDGIVKKGEITGIKGDYEYGNIALLYGIAERTVIPVLREIYPTMWKRIISYVILRNIQPLPMKSVHYLYEKTYLPRIMDESMSPDSLSRMLSSLPEDQSIRVMKKLTEKGEYVLMDSTAIFSRSENMSFLELGHNSKGMHLPQINVMMLFSSTRTMPTFVRILPGSIRDASAMSKTIDMAGVDRCVIVADKGFFSAINVKKLKRRHLSYIIPLRRNSSLIPEPEHFMGVFSYDGKPVKYWKRENDVYIFEDPVLRSEEEKDFLLRIEENKRSRKQYDENEINFGKLYLLSDLNEEPERIYRLYKQREYVEYAFNVYKNDLEADRSYLRDDHMLFTYMFLNLLSLYLHFQILNMIDGKYSVKDILLILSRIKMYRTEKGEIMSEIPKKANDLVSTLNIDLDLLRKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CE1</accession>
    <name>Q97CE1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-binding protein HU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0171266</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916416.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CE1"/>
    <dbReference type="SMR" id="Q97CE1"/>
    <dbReference type="STRING" id="273116.gene:9380931"/>
    <dbReference type="PaxDb" id="273116-14324375"/>
    <dbReference type="GeneID" id="1441646"/>
    <dbReference type="KEGG" id="tvo:TVG0171266"/>
    <dbReference type="eggNOG" id="arCOG05336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_3_3_2"/>
    <dbReference type="OrthoDB" id="55723at2157"/>
    <dbReference type="PhylomeDB" id="Q97CE1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="entry name" value="IHF-like DNA-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="DNA-BINDING PROTEIN HU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175:SF3">
        <property type="entry name" value="DNA-BINDING PROTEIN HU-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="IHF-like DNA-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="90" mass="10035" checksum="2230EE748944CAC9" modified="2001-10-01" version="1">MVGISELSKDVAKKANTTQKVARTVIKSFLDEIVAQANGGQKINLAGFGIFERRTQGPRKARNPQTKKVIEVPSKKKFVFRASSKIKYQQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CG6</accession>
    <name>RNH2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease HII</fullName>
            <shortName evidence="1">RNase HII</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhB</name>
        <name type="ordered locus">TV0135</name>
        <name type="ORF">TVG0142583</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase HII family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59277" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59277.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054063.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CG6"/>
    <dbReference type="SMR" id="Q97CG6"/>
    <dbReference type="STRING" id="273116.gene:9380904"/>
    <dbReference type="PaxDb" id="273116-14324349"/>
    <dbReference type="GeneID" id="1441619"/>
    <dbReference type="KEGG" id="tvo:TVG0142583"/>
    <dbReference type="eggNOG" id="arCOG04121">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_0_4_2"/>
    <dbReference type="OrthoDB" id="33866at2157"/>
    <dbReference type="PhylomeDB" id="Q97CG6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07180">
        <property type="entry name" value="RNase_HII_archaea_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_A">
        <property type="entry name" value="RNase_HII_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020787">
        <property type="entry name" value="RNase_HII_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF23">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000111673" description="Ribonuclease HII">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-2" evidence="2">
        <location>
            <begin position="4"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="212" mass="24293" checksum="D050351673E74464" modified="2003-08-29" version="2">MHEEVQCGIDEAGRGPVIGPMVISIVCCKEEYLREIGVKDSKMLSRKRREYLFDKITKNCAYSYVVVSPETLNKDMASESLNKIEENYILELLSKAEGIVYIDCFDVIEERAEKYIREKSGKEVVCKHKADSIYPAVSAASIVSKVIRDREIDKIAEKYGFFGSGYPSDPRTIDFLRKAMQQGLDLRSVARIHWETYKKIKEDVESGQKKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CH4</accession>
    <name>Q97CH4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type II methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">N-4 cytosine-specific methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0134727</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916381.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CH4"/>
    <dbReference type="STRING" id="273116.gene:9380895"/>
    <dbReference type="REBASE" id="685114">
        <property type="entry name" value="M.TvoORF3675P"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324341"/>
    <dbReference type="GeneID" id="1441611"/>
    <dbReference type="KEGG" id="tvo:TVG0134727"/>
    <dbReference type="eggNOG" id="arCOG00108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_796013_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CH4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0680">Restriction system</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="4">
        <location>
            <begin position="5"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="348" mass="39943" checksum="B230DA496B84D7B8" modified="2001-10-01" version="1">MQGQSSENLGYVTQKPEALLKRIIEASSLPDSIVADFFAGSGTLGAVAEKLGRKWIMCDVGKPACMIMRKRLSDLNARPFLYQSIGDYQKEQFKRSQFRRIGDLAHVVIKLYGAIPFPMQDGIPNNLGYIKQSKTLVYVDSPARITGYSTLKGAQKLRRSVMGRWEKIVVLGWNFDNDIGRIIESLNDDKLEVLVIPPDLLEKLRNKEDCDDLIKNGKVKFSSLRYLSVKPIRRKTVDEAQEELEIELENYVLLSPDALPLDKDDKEKLEKVIEEDPLSLIEYWSIDPDYDGEVFRSKWQDYRENHRNSFKVNRKVKLLVPRTGKKRRVCVRAVDVLGFESMAIQDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CJ8</accession>
    <name>Q97CJ8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0108190 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0108190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2010-04" db="PDB data bank">
            <title>Crystal structure of a Putative nitrate transport protein (TVN0104) from THERMOPLASMA VOLCANIUM at 1.35 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916360.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="3MST">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.35 A"/>
        <property type="chains" value="A=1-225"/>
    </dbReference>
    <dbReference type="PDBsum" id="3MST"/>
    <dbReference type="AlphaFoldDB" id="Q97CJ8"/>
    <dbReference type="SMR" id="Q97CJ8"/>
    <dbReference type="STRING" id="273116.gene:9380871"/>
    <dbReference type="PaxDb" id="273116-14324317"/>
    <dbReference type="DNASU" id="1441590"/>
    <dbReference type="GeneID" id="1441590"/>
    <dbReference type="KEGG" id="tvo:TVG0108190"/>
    <dbReference type="eggNOG" id="arCOG03693">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1307872_0_0_2"/>
    <dbReference type="OrthoDB" id="56574at2157"/>
    <dbReference type="EvolutionaryTrace" id="Q97CJ8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024533">
        <property type="entry name" value="DUF3834"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12916">
        <property type="entry name" value="DUF3834"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="3" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="3MST"/>
        </source>
    </evidence>
    <sequence length="225" mass="25148" checksum="F622A547569376E3" modified="2001-10-01" version="1">MDVGMPFAGPVSFPLLVIEEELPFRIHNICSETGKFDVVLDSITNMPKYGLKIFAGVRIDMYSILGDESSGRIYTLRKGTLADFNARILAYYDKAQVINADGDTCIKMANEGYSALVGNEISIGKSFRNRMKELGLDLPSCAMASTRRIDEVIEAYEQGIDFIKNNHERAAEIISKKSGYYSEEVMKKIIGIYGHEVTKKRAELVGSRELYSRVVPELNDIEIIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CK6</accession>
    <name>COMB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Probable 2-phosphosulfolactate phosphatase</fullName>
            <ecNumber>3.1.3.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">comB</name>
        <name type="ordered locus">TV0095</name>
        <name type="ORF">TVG0100132</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15597"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58738"/>
            <dbReference type="EC" id="3.1.3.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ComB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.71"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916352.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CK6"/>
    <dbReference type="SMR" id="Q97CK6"/>
    <dbReference type="STRING" id="273116.gene:9380863"/>
    <dbReference type="PaxDb" id="273116-14324309"/>
    <dbReference type="GeneID" id="1441582"/>
    <dbReference type="KEGG" id="tvo:TVG0100132"/>
    <dbReference type="eggNOG" id="arCOG04871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070028_0_0_2"/>
    <dbReference type="OrthoDB" id="146693at2157"/>
    <dbReference type="PhylomeDB" id="Q97CK6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050532">
        <property type="term" value="F:2-phosphosulfolactate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1560.10">
        <property type="entry name" value="ComB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00490">
        <property type="entry name" value="ComB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005238">
        <property type="entry name" value="ComB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036702">
        <property type="entry name" value="ComB-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002057">
        <property type="entry name" value="PRK00886.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37311">
        <property type="entry name" value="2-PHOSPHOSULFOLACTATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37311:SF1">
        <property type="entry name" value="2-PHOSPHOSULFOLACTATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04029">
        <property type="entry name" value="2-ph_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142823">
        <property type="entry name" value="ComB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000081462" description="Probable 2-phosphosulfolactate phosphatase">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="212" mass="23936" checksum="D67465756C8C4A29" modified="2001-10-01" version="1">MIRIADGRKEENWSSINIIIDIFRSTTTIPVILSRGARYIVPFKDVTSALNFKRKNKNVVLIGEKYGIKPPFFDYDNSPAQIINADLEGKIIAFTSTNGMYVLSRIKRGRILFSSLVNMSATIKKVKGKDDILVVPSNRPIGKAVEDNIFAEMLKLALEGKNYDREILVNRIRETKENTVVSISTQDLEICLKLDLLDCVPEYIEGKIVNDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CL2</accession>
    <name>Q97CL2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Formiminotransferase cyclodeaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0091933</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916346.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CL2"/>
    <dbReference type="STRING" id="273116.gene:9380857"/>
    <dbReference type="PaxDb" id="273116-14324303"/>
    <dbReference type="GeneID" id="1441576"/>
    <dbReference type="KEGG" id="tvo:TVG0091933"/>
    <dbReference type="eggNOG" id="arCOG05395">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088419_0_1_2"/>
    <dbReference type="OrthoDB" id="56828at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.680">
        <property type="entry name" value="Formiminotetrahydrofolate cyclodeaminase monomer, up-and-down helical bundle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007044">
        <property type="entry name" value="Cyclodeamin/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036178">
        <property type="entry name" value="Formintransfe-cycloase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04961">
        <property type="entry name" value="FTCD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101262">
        <property type="entry name" value="Methenyltetrahydrofolate cyclohydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclodeaminase/cyclohydrolase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="204" mass="22913" checksum="4C1A37F352B8DEFD" modified="2001-10-01" version="1">MESYNNFIERLASPSPAPGGGAASAFVSIVASALISMVSQITMGKKGYEQYEDEMKEIISENSKIKEELEKLIEEDEEAFNSIMAAIKMPKITEEEKKEREKKLQSALRHGIEVPWRIAARSFDLLKLSEKLVEHGNKNAVTDAGSAASILHSAIEAALYNVKINLKSVKDQEYVSDQKMKIKLFLENSDEVYERIKKKLEAML</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-02-11" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CL5</accession>
    <name>MOAE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Molybdopterin synthase catalytic subunit</fullName>
            <ecNumber>2.8.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>MPT synthase subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Molybdenum cofactor biosynthesis protein E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Molybdopterin-converting factor large subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Molybdopterin-converting factor subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">moaE</name>
        <name type="ordered locus">TV0086</name>
        <name type="ORF">TVG0086076</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by MoaD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + cyclic pyranopterin phosphate + H2O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26333"/>
            <dbReference type="Rhea" id="RHEA-COMP:12202"/>
            <dbReference type="Rhea" id="RHEA-COMP:19907"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58698"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:90778"/>
            <dbReference type="ChEBI" id="CHEBI:232372"/>
            <dbReference type="EC" id="2.8.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of 2 MoaD subunits and 2 MoaE subunits. Also stable as homodimer. The enzyme changes between these two forms during catalysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.12"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916343.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CL5"/>
    <dbReference type="SMR" id="Q97CL5"/>
    <dbReference type="STRING" id="273116.gene:9380853"/>
    <dbReference type="PaxDb" id="273116-14324300"/>
    <dbReference type="GeneID" id="1441573"/>
    <dbReference type="KEGG" id="tvo:TVG0086076"/>
    <dbReference type="eggNOG" id="arCOG00534">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089568_1_2_2"/>
    <dbReference type="OrthoDB" id="45235at2157"/>
    <dbReference type="PhylomeDB" id="Q97CL5"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030366">
        <property type="term" value="F:molybdopterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00756">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="entry name" value="Molybdopterin biosynthesis MoaE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23404">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="Molybdopterin synthase subunit MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000163110" description="Molybdopterin synthase catalytic subunit">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="36"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="137" mass="15488" checksum="DB2745386C5F9466" modified="2001-10-01" version="1">MINTYVEITEKDINPLDLINRVRRPDAGAIVTFEGTVRNDSDGVRVTALYYEAYKEMAEMQIADLIDEAKKKYNILDAAVCHRIGLVGLTEDSVVISVSSAHRSSAFEACRYIIDTIKERVPIWKRDILENGNGSWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CM4</accession>
    <name>Q97CM4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribonucleotide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0077159</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916334.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CM4"/>
    <dbReference type="STRING" id="273116.gene:9380843"/>
    <dbReference type="PaxDb" id="273116-14324291"/>
    <dbReference type="DNASU" id="1441564"/>
    <dbReference type="GeneID" id="1441564"/>
    <dbReference type="KEGG" id="tvo:TVG0077159"/>
    <dbReference type="eggNOG" id="arCOG04273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_2_2"/>
    <dbReference type="OrthoDB" id="39225at2157"/>
    <dbReference type="PhylomeDB" id="Q97CM4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005021">
        <property type="entry name" value="PRK06425.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF1">
        <property type="entry name" value="THREONINE-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="3">
        <location>
            <begin position="28"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="335" mass="38388" checksum="8C45762990BBA27A" modified="2001-10-01" version="1">MRSHGGDVYKVSPNYDRITDMSSNVNPFLDLGKYSLYPEISYTDIEEKLRVYTKSTNMGIVIGPGLTHLIYKYFEFYQVKKPIIAMPNFSEYKGIAEDRHLRFSAIPSYIIEKDPLILKNFDHDALFITSPSNPFGELFPESTLETVFEIEEKRGRRVFLDGAFMDFVPNYAESIVQSSRSYFNVTIGRSLTKILAIPSLRAGYLIMERHEADLFRKAMEPWSICQPAIDFIRSIDINEVAKNSVALVNGEREYLISELKARGIKVIGNPSANYIVISMPRTDEFYNFMLSKGILVRLLDDYDYVGRCLVRLAIKTRSQNNAFLNALDNFMSSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CN7</accession>
    <name>Q97CN7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0065973</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916321.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CN7"/>
    <dbReference type="STRING" id="273116.gene:9380830"/>
    <dbReference type="PaxDb" id="273116-14324278"/>
    <dbReference type="GeneID" id="1441551"/>
    <dbReference type="KEGG" id="tvo:TVG0065973"/>
    <dbReference type="eggNOG" id="arCOG01997">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079909_0_0_2"/>
    <dbReference type="OrthoDB" id="10856at2157"/>
    <dbReference type="PhylomeDB" id="Q97CN7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00427">
        <property type="entry name" value="NAAT family transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508:SF1">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="212" mass="22861" checksum="E18E58E9ACD1BEAB" modified="2001-10-01" version="1">METLLFLVTVFIALFALVNPIGALPVLIAVTEGYSSEDFDRVVKNATVTEALMLFGFMFIGIYIFQVLGINIDDFKVAGGVLLFKVAFDMLQGRTSTTKLTPVEKEETAERSSIGIAPIGTPLLAGPGSITTAIIYFNSYSTNLTDKVLVIVAVFLVIVVSYLMLHYSSILFKFLGNTGSLLISRIMGLLLAAIAVDFMVTGLAYVVARILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CP0</accession>
    <name>Q97CP0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sodium-dependent phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0063671</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CP0"/>
    <dbReference type="STRING" id="273116.gene:9380827"/>
    <dbReference type="PaxDb" id="273116-14324275"/>
    <dbReference type="KEGG" id="tvo:TVG0063671"/>
    <dbReference type="eggNOG" id="arCOG02267">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_932616_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CP0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="31550" checksum="170E9B694EA142BC" modified="2001-10-01" version="1">MLLSFLLVILVAGNNTSAVSGTLIGSRMVGRYYGLLMTAVGYVAGYLIEGQFLKKAVSVLLPFPPEVVIYAILASFIIFVVAFFVKVPLSLTMALVGSSIGLSLKYGVNLPKSFLLSIFVAWILAPIISVVLSYYLNVVMSGKNYGNIWKYASAVKLLLIFTALFTAFTLGANTIGFIGNVIGFNTENNIVIVAAVFLGTFMFSSGVMKSVSQNMYMLKYTNAFTSQIVSALAVEVSTLFSIPMSNTQTLTSSVLGAGLSYKAKAIYLRPFLYIVVMWIISPILGLVLTYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CP4</accession>
    <name>Q97CP4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">aminomethyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0060439</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054057.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CP4"/>
    <dbReference type="STRING" id="273116.gene:9380822"/>
    <dbReference type="PaxDb" id="273116-14324271"/>
    <dbReference type="GeneID" id="1441544"/>
    <dbReference type="KEGG" id="tvo:TVG0060439"/>
    <dbReference type="eggNOG" id="arCOG00756">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_10_2_2"/>
    <dbReference type="OrthoDB" id="2001at2157"/>
    <dbReference type="PhylomeDB" id="Q97CP4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1400:FF:000001">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.110">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1250.10">
        <property type="entry name" value="Aminomethyltransferase fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF2">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="8">
        <location>
            <begin position="5"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="9">
        <location>
            <begin position="285"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006487-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="359" mass="40243" checksum="D96600AFB1B7C17A" modified="2001-10-01" version="1">MKTVLYDEHVKLNAHFTDFNGWDMPLYYKSIIEEHMSVRKHAGIFDVSHMGDITVRGREAGKFLDHMFPTKVSSLNDGECIYTAFLNEKGQMIDDTIVYRMSSDSYFFVPNAGTTDKIYEWVTKNSKGFDVKIENVSSKISSIALQGPESESVISELGFSYPGYFKFTYVNGKYKNAITGKNDIIISGTGYTGEKGVEFIIPNEYAVELWRKLLSLVEKRSGLPCGLGSRDTLRMEKGMLLSGHDFNENRDPYEASISFIVNNDGDFIGKKSLEDRRKEDKQIFRGFILSDGIPRSGNSVKVNGKVVGTLTSGSISPVLNKGIALGYIDKEYSKEGTKVFVEIRGKDHEATVSRPKIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CQ6</accession>
    <name>Q97CQ6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Exodeoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0048222</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916303.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CQ6"/>
    <dbReference type="STRING" id="273116.gene:9380810"/>
    <dbReference type="PaxDb" id="273116-14324259"/>
    <dbReference type="GeneID" id="1441532"/>
    <dbReference type="KEGG" id="tvo:TVG0048222"/>
    <dbReference type="eggNOG" id="arCOG02207">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027539_1_3_2"/>
    <dbReference type="OrthoDB" id="146626at2157"/>
    <dbReference type="PhylomeDB" id="Q97CQ6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09073">
        <property type="entry name" value="ExoIII_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00195">
        <property type="entry name" value="exoDNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="9">
        <location>
            <begin position="8"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="8">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="8">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="252" mass="29243" checksum="61A41AFB95AFC79B" modified="2001-10-01" version="1">MSETKKFLSWNVNGLRAVIKSGGLDLIRNGDYFAIAMQETKVDVKSVPEEMYHLGYHVYNNPAKRKGYSGTMTLSREKPIDVSYGFESEEGRILNLEFDNFYFINAYFPNSQHGLTRLDLKLDFDKKFLGYANDLRKKKPLIICGDFNVAHEEIDIARPKDNEHNAGFTIEERTWMSQFLSSGYVDTFRLFTTDGGHYSWWSYRFNAREKNIGWRIDYFVVSDDIKDKVKASRILENVKGSDHAPVELEIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CS0</accession>
    <name>Q97CS0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Probable cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cobQ</name>
        <name evidence="10" type="ORF">TVG0030868</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CS0"/>
    <dbReference type="STRING" id="273116.gene:9380796"/>
    <dbReference type="PaxDb" id="273116-14324245"/>
    <dbReference type="KEGG" id="tvo:TVG0030868"/>
    <dbReference type="eggNOG" id="arCOG00105">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019250_2_2_2"/>
    <dbReference type="PhylomeDB" id="Q97CS0"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05389">
        <property type="entry name" value="CobQ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047045">
        <property type="entry name" value="CobQ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001989">
        <property type="entry name" value="PRK00784.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="COBYRIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4 7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="8">
        <location>
            <begin position="19"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="CobB/CobQ-like glutamine amidotransferase" evidence="9">
        <location>
            <begin position="259"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="481" mass="53379" checksum="FADEF67A7CB97BA7" modified="2001-10-01" version="1">MLQLFNLKLYDQLMKMIQVLGTSSGAGKTTVSMALCRILSRQGYSVAPFKAMNMSLNSVIVEGEYEIARAQWLQAIAAKTDPSRYMNPLLLKPEGDGTSQLIVLGKSIGKKGIKEYYDYLRSKGKGIVKESIERLPEKYDVIIAEGAGSPAEINLLDSDIANSFVSSIYGTPAILVGDIDRGGVFASIYGTLMLMPNSELVKWIIINKMRGDVSMLNPGIERLENLISRKVIGILPYQSGFLPGEDSFDYEKPPVENDEICVIRYPFMENYSDVDPLVLSGKGFTFVTEKNINALDNAKLIILPGSKNVPADLEYVKNTGIAEKLSERRGKALILGICGGYQILGSRIDYYGKSMSCLSFLKCHTEYREEKTTRSIRFKFAEGKYWSDGYEIHYGEVINDGEKPMNLTELGYEGCISEDGTVFGTNIHNILGNSDFFLRAVGEQSTGLTARLESSIDAFADTFEKNVNLSEIMEYLEEKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CT4</accession>
    <name>Q97CT4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl-CoA hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0015475</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916274.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CT4"/>
    <dbReference type="STRING" id="273116.gene:9380782"/>
    <dbReference type="PaxDb" id="273116-14324231"/>
    <dbReference type="GeneID" id="1441502"/>
    <dbReference type="KEGG" id="tvo:TVG0015475"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_2"/>
    <dbReference type="OrthoDB" id="27846at2157"/>
    <dbReference type="PhylomeDB" id="Q97CT4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="251" mass="28260" checksum="0FA1A0142CC297CA" modified="2001-10-01" version="1">MSSPNYRNISLEDHEGIRIVTIRRENSLNPLNLDTLEEIEDAVRESGKVVVLKGSEKAFSAGADINNFLDMSDRDAFHFSDRGQQVMDSISDYERPVIAAVHGYALGGGFELALACDFRISDVKTKYGFPEVNLGIMPGFGGTQRIIDIAGKSYGMYLVMTGKTIDEQEALKHGIVDSVSEKYLDLAIELAKELSEKPATSIRYIKEVMNRRDKEGYMMERERFALTFKTEDHLEGIRAFKEKRKAVFKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q978B0</accession>
    <name>Q978B0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell division inhibitor [MinD]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1560863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917736.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978B0"/>
    <dbReference type="STRING" id="273116.gene:9382321"/>
    <dbReference type="PaxDb" id="273116-14325746"/>
    <dbReference type="GeneID" id="1442196"/>
    <dbReference type="KEGG" id="tvo:TVG1560863"/>
    <dbReference type="eggNOG" id="arCOG00586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_1_4_2"/>
    <dbReference type="OrthoDB" id="36110at2157"/>
    <dbReference type="PhylomeDB" id="Q978B0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02042">
        <property type="entry name" value="ParAB_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000285">
        <property type="entry name" value="Sporulation initiation inhibitor Soj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050678">
        <property type="entry name" value="DNA_Partitioning_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696:SF52">
        <property type="entry name" value="PARA FAMILY PROTEIN CT_582"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009320">
        <property type="entry name" value="Nuc_binding_HP_1000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="251" mass="27771" checksum="9D1D6F5C58D03BFE" modified="2001-10-01" version="1">MIISIANQKGGCGKTTTAVNLGSVLARKHKVLLIDIDPQGNLTTSFGVNKGELNRTMYDVMLDGGLEKAILRKDSIDIVPSIIDLAGAEVQLSGRMGREYILANELSKLSRRYDFIIIDTPPSLGVFTINALVASDYVLIPVQAEFFALEGLTQLLSVVDLVNTRLGRTLKILGMVVTMFNSRTKSSNEVLEDVRKHYSKHLFRTIIPRNVTVTDSTMTGEPVVIYRKDASASKSYVELAKEVENRLRVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q978B2</accession>
    <name>Q978B2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1559742 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1559742</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978B2"/>
    <dbReference type="PaxDb" id="273116-14325744"/>
    <dbReference type="KEGG" id="tvo:TVG1559742"/>
    <dbReference type="HOGENOM" id="CLU_3003374_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="56" mass="6560" checksum="99C0E4568EC49098" modified="2001-10-01" version="1">MAKMDENTYQCEICKLHYKDESDAKKCQDWCSQHNSCNLEITSRSLEASKSRHTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q978D4</accession>
    <name>Q978D4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formate hydrogenlyase subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1535075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917714.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978D4"/>
    <dbReference type="STRING" id="273116.gene:9382297"/>
    <dbReference type="PaxDb" id="273116-14325722"/>
    <dbReference type="GeneID" id="1442174"/>
    <dbReference type="KEGG" id="tvo:TVG1535075"/>
    <dbReference type="eggNOG" id="arCOG01537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_8_1_2"/>
    <dbReference type="OrthoDB" id="371891at2157"/>
    <dbReference type="PhylomeDB" id="Q978D4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050616">
        <property type="entry name" value="CPA3_Na-H_Antiporter_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005046">
        <property type="entry name" value="PRK06459.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43373">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43373:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="469"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="2">
        <location>
            <begin position="95"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="589" mass="64508" checksum="A38D9BF0A1C008D0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q978F5</accession>
    <name>RPO11_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo11</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo11</name>
        <name evidence="1" type="synonym">rpoL</name>
        <name type="ordered locus">TV1462</name>
        <name type="ORF">TVG1510824</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60604" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60604.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917691.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978F5"/>
    <dbReference type="SMR" id="Q978F5"/>
    <dbReference type="STRING" id="273116.gene:9382274"/>
    <dbReference type="PaxDb" id="273116-14325701"/>
    <dbReference type="GeneID" id="1442151"/>
    <dbReference type="KEGG" id="tvo:TVG1510824"/>
    <dbReference type="eggNOG" id="arCOG04111">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090381_5_2_2"/>
    <dbReference type="OrthoDB" id="24205at2157"/>
    <dbReference type="PhylomeDB" id="Q978F5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002241">
        <property type="entry name" value="PRK01146.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000149342" description="DNA-directed RNA polymerase subunit Rpo11">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="111" mass="13089" checksum="2D91AD609CE6D285" modified="2002-05-27" version="2">MQRERTNETSLRVVSKEKDSIIVEMINYDNTLLRTLVEEILKDDQVIEARYYIKHPIIDNPQIYVKVKSGKPQAAIKRSIRKLSKLYEDLGAQVEKEIEKYRSNHIIKTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q978K0</accession>
    <name>Q978K0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SUF system FeS cluster assembly SufBD core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1462163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917647.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978K0"/>
    <dbReference type="STRING" id="273116.gene:9382224"/>
    <dbReference type="PaxDb" id="273116-14325654"/>
    <dbReference type="GeneID" id="1442105"/>
    <dbReference type="KEGG" id="tvo:TVG1462163"/>
    <dbReference type="eggNOG" id="arCOG01715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_680787_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978K0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43575">
        <property type="entry name" value="PROTEIN ABCI7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43575:SF1">
        <property type="entry name" value="PROTEIN ABCI7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="1">
        <location>
            <begin position="153"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="404" mass="45826" checksum="6C48FA455D3B9C36" modified="2001-10-01" version="1">MIEQIRDLINDRRNDGLHEYRQDNFIQFMKSPVQPYKESPTRLRYVDFTDDLVEKMVLGEPEDDAKGYGQGDEDFAFVNGKLMVYNGKGCIVEPIREAERKYPNLIFDYTDKENVDDRYEYLINAGFYDGFLVYLPPNKSSEIKAVDFAKSTSSFAAKNLIIVGEGSSLNFTRYIVGGGEGNGAQGDNTYIFLSRNSSIVYSQVQDRPKTVDGIQFLKTFMEDNTQATIHHVENGYNKSIIMTESYQKGNFTNYQLRGVSFTRGLQQMDLRDNTIQIGTHSIANIYVRGIVREKSLTMHRGNIDIKESGRKAEGYYDTRILLLSKEAYANTKPALLINNNDVKSKHASAVTNIDEESLFYLRSRGIDEETAKNLIIEGFTEHVVEGAPDAVRNAVRKFAGISED</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="144" xmlns="https://uniprot.org/uniprot">
    <accession>Q978L4</accession>
    <name>Q978L4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 20">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="13">IMP dehydrogenase</shortName>
            <shortName evidence="13">IMPD</shortName>
            <shortName evidence="13">IMPDH</shortName>
            <ecNumber evidence="13 20">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">guaB</name>
        <name evidence="22" type="ORF">TVG1445839</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="22 23" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13 20">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13 20">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 13">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 19">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="13 20"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917633.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978L4"/>
    <dbReference type="STRING" id="273116.gene:9382210"/>
    <dbReference type="PaxDb" id="273116-14325640"/>
    <dbReference type="GeneID" id="1442091"/>
    <dbReference type="KEGG" id="tvo:TVG1445839"/>
    <dbReference type="eggNOG" id="arCOG00612">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_2"/>
    <dbReference type="OrthoDB" id="21361at2157"/>
    <dbReference type="PhylomeDB" id="Q978L4"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000003">
        <property type="entry name" value="GMP reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF111">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 13 20" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="4 13 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13 16" id="KW-0520">NAD</keyword>
    <keyword evidence="9 13 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 13 17" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 13 20" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="21">
        <location>
            <begin position="97"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="21">
        <location>
            <begin position="155"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="13 14">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13 14">
        <location>
            <position position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="246"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 16">
        <location>
            <begin position="295"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="335"/>
            <end position="337"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="358"/>
            <end position="359"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="382"/>
            <end position="386"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BAB60543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="485" mass="52727" checksum="7919D260618917AF" modified="2001-10-01" version="1">MYRDKFSNITEGYTFDDVLILPMKTGVEPKNVDVSSRLSRRINVKVPIVSSPMDTVTEEAMAIAMARYGAFGIIHRNQPREKEVEMVRRVKREETIIIRDVYTVSPETPIEVARTIMKTKNIAGLPVLKEEKLVGILTKRDLEFAKQGSTVSDVMVKNVITAPENVDLEDAINILHKNRIEKLPLVDKDNHLVGLITAKDIITRQRFPDATRDDEGQLMVGAAVGPFDLDRAIELERAGADLIVVDTAHADNENVLSSIKRMRKEISVDIVAGNIATAGAAEDLISCEVDGLRVGIGPGSICTTRIVAGVGVPQLTAISEVAEVAKEYGIPVIADGGIRYSGDIVKAIAAGADSVMLGSMLAGTEESPGQEMIINGRKYKAYRGMGSIGALSTGISDRYSKLGNGFIAEGVEGAVPYRGRVDEVLFQLVGGLRTGMGYVGAENIGKLKERARFVRISSNGLRESHPHDIRLISEPPNYQISNYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q978L7</accession>
    <name>Q978L7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase II small subunit</fullName>
            <shortName evidence="15">Pol II</shortName>
            <ecNumber evidence="15">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Exodeoxyribonuclease small subunit</fullName>
            <ecNumber evidence="15">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">polB</name>
        <name evidence="17" type="ORF">TVG1442259</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 19">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="15"/>
    <dbReference type="EC" id="3.1.11.1" evidence="15"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978L7"/>
    <dbReference type="STRING" id="273116.gene:9382207"/>
    <dbReference type="PaxDb" id="273116-14325637"/>
    <dbReference type="KEGG" id="tvo:TVG1442259"/>
    <dbReference type="eggNOG" id="arCOG04455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027850_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q978L7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042575">
        <property type="term" value="C:DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07386">
        <property type="entry name" value="MPP_DNA_pol_II_small_archeal_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04490">
        <property type="entry name" value="PolII_SU_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00325">
        <property type="entry name" value="DNApol_II_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011149">
        <property type="entry name" value="Pol2_small_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003118">
        <property type="entry name" value="PRK04036.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000803">
        <property type="entry name" value="Arc_Pol2_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="16">
        <location>
            <begin position="213"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAB60540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="466" mass="52626" checksum="851BF47FF78BF175" modified="2001-10-01" version="1">MIMADLDKASLLEYFTKNGVLVSPSALEKIIKYSSSSLIEDLVKNSDGYIDDRIVEAALKPKREMNDFEVYLPEVRFNSSVEDFRKMFVSKYEKLSRIISASSSMRGSISIKTAKHSQGQVKVIGMVSEVSMTKNGHKRVLIEDLDDSIQVFILKDRGIINEVILEDEVIGIIGNVSSSSKDPVIFADEIVRPDIPYRILDETKHEPVFVASLSDIHIGSKTFRKNEFQDMIKWFESSDDTSLSTKYLILSGDVVDGIGVYPGQENDLELLNPLDQYEALASFVSQIPEDITVFIMPGNHDTVRLSEPQPVFPEKIRKMFPSNAVFLPNPYNLKLEGKNVLVYHGMSLNDMIELIPGANYDSIGKAIEAILVRRHLSPRYGGNTPLIPSSVDYHVIEEVPDIFITGHIHSHYIGNYKGVRYVNSSTWQSQTEYQKMMNFNPKPSKLTLFDLYSRTVIVKDFDKSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q978M1</accession>
    <name>Q978M1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1440802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917627.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978M1"/>
    <dbReference type="STRING" id="273116.gene:9382202"/>
    <dbReference type="PaxDb" id="273116-14325633"/>
    <dbReference type="GeneID" id="1442085"/>
    <dbReference type="KEGG" id="tvo:TVG1440802"/>
    <dbReference type="eggNOG" id="arCOG00041">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083583_0_0_2"/>
    <dbReference type="OrthoDB" id="56536at2157"/>
    <dbReference type="PhylomeDB" id="Q978M1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.20">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="214" mass="23220" checksum="5E14DEBD1A5A444D" modified="2001-10-01" version="1">MVTFIDRYEAGKQLAKKITNLSGKCTVTGIARGGIAVAYQVSASIGLPLTTIIVKKIGHPADEEFAIGAVAEGPEPYVYKGEYALSVEPAIFEAFAAEKKKEVNSVRRILGQENSVFYKNWDCVILVDDGVATGSSMIGAIRSLKLNVSKNIIAAAPVFSEEAYVAVRSEGVSIAYVIIPFDFEAVSEFYRDFSEITYADVLEIIRKAYANNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q978M6</accession>
    <name>Q978M6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1434889</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917622.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978M6"/>
    <dbReference type="STRING" id="273116.gene:9382197"/>
    <dbReference type="PaxDb" id="273116-14325628"/>
    <dbReference type="GeneID" id="71201332"/>
    <dbReference type="KEGG" id="tvo:TVG1434889"/>
    <dbReference type="eggNOG" id="arCOG02689">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163857_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978M6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF34">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="26"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="238" mass="26187" checksum="B799BE8DCB99F6CC" modified="2001-10-01" version="1">MVSEEQIKNIIARENRIPVWSLPYTFLAIIGIGYFFTFYDISDIGLAMPAIASQFHIGTSLSLFIALSVGLIGYSIGSYLIGSLADVYGRYRSMILTMGLTALGSFGDAISINVPELTIFRFITGLGLGADLNLVSSYISEFAPPAVRGRITVYTFLIGILGQAITPFIGLHLVPVYYNGWRYLFGIGAVIALIALFLRFELPESPRWLASKAHNIEKAEKIIQIMEHTALKKVKSLP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q978N1</accession>
    <name>Y1384_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein TV1384</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV1384</name>
        <name type="ORF">TVG1431058</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917617.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978N1"/>
    <dbReference type="SMR" id="Q978N1"/>
    <dbReference type="STRING" id="273116.gene:9382192"/>
    <dbReference type="PaxDb" id="273116-14325623"/>
    <dbReference type="GeneID" id="1442075"/>
    <dbReference type="KEGG" id="tvo:TVG1431058"/>
    <dbReference type="eggNOG" id="arCOG01336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095686_1_1_2"/>
    <dbReference type="OrthoDB" id="55941at2157"/>
    <dbReference type="PhylomeDB" id="Q978N1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.150">
        <property type="entry name" value="Hypothetical protein ph0010, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00645">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027623">
        <property type="entry name" value="AmmeMemoSam_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023472">
        <property type="entry name" value="Uncharacterised_MJ0810"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04335">
        <property type="entry name" value="AmmeMemoSam_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142391" description="Protein TV1384">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="AMMECR1" evidence="1">
        <location>
            <begin position="11"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00645"/>
        </source>
    </evidence>
    <sequence length="201" mass="22432" checksum="AD7FFB0A0D6F2C48" modified="2001-10-01" version="1">MDSEQINVNLDIGAKAVMLARRAAAAKLNNEKLPEVPDDPIFHEKHGVFTTINTYPDNQLRGCIGFPEPYYELGEGIIKSSIYAATDDPRFDPMEPDELNRVTFELSILTVPQEVTVNPEERPKAITVGKDGIIAVYNGASGLLLPQVATEYRMSAEEFLEALCEKAGLWQGCWKYKKVKISKFQAIVFGEIEPNGKVEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Q7</accession>
    <name>Q978Q7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Ferredoxin oxidoreductase [KOR] beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG1405258</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Q7"/>
    <dbReference type="STRING" id="273116.gene:9382166"/>
    <dbReference type="PaxDb" id="273116-14325597"/>
    <dbReference type="KEGG" id="tvo:TVG1405258"/>
    <dbReference type="eggNOG" id="arCOG01599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978Q7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
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        <source>
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</entry>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
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            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
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                <person name="Kanehori K."/>
                <person name="Amano N."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <source>
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        </source>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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        </source>
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        </source>
    </evidence>
    <sequence length="114" mass="13221" checksum="7641838B2388D964" modified="2001-10-01" version="1">MCLVTELGEAKKCISIDESDIELIDSDEDLRYHFSVLFDTCQKYQDLLRQKAELESRYAAIKFSLYSALEDMKRVAIQISAMVEDAKMKDIDVPEGVIKQAVYLIDRYMVIRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
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                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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            <dbReference type="PubMed" id="11121031"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="GO" id="GO:0004143">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303:SF1">
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
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    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="34574" checksum="AD0E8E4DBE8C91FB" modified="2001-10-01" version="1">MIGAVIEAGVGITAALISLIEDKQNFLKKYEVSLIILPILVIFFHSMLIVPIYVFSIISGTYLYSKKFYYPFLLLFVSVFLIYIVYGINYPWKSTDIAISAGMIVVMVFDSREKEYVKQNEMNRGTDRKKEIRRDYVQILAGAVIILLIYYYGIDVSRILVTFGSLVLYVTGNFLAGRPDLLLGKLLNSLERSSTKLGIGSLWFASGILIAYSLHDSTAVVMMIVFSIAVGDSLATIIGTSVSSPKLPINRKKSAAGSIAFFAASAAFAIFVLGYAGLFYAAVATITEAVSGFPLDDNLTVPFILSILAYVSKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q978V6</accession>
    <name>Q978V6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1351159 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1351159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917544.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978V6"/>
    <dbReference type="STRING" id="273116.gene:9382117"/>
    <dbReference type="PaxDb" id="273116-14325548"/>
    <dbReference type="GeneID" id="1441426"/>
    <dbReference type="KEGG" id="tvo:TVG1351159"/>
    <dbReference type="eggNOG" id="arCOG02206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1297527_0_0_2"/>
    <dbReference type="OrthoDB" id="57101at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="beta-trefoil_Ricin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="231" mass="26022" checksum="0C9BB9A4BE0BC597" modified="2001-10-01" version="1">MPKDTEEPLVKVPEFDEKQFLFDEKERAKSIVMIFIIGAVIGILSGYLQLIGYWYFSVLLLLIVLVFSEYILKGLGVKVSKKTTHRILFIGELFLTWIVFWILVLNPPFSHVSGPEISNIQMFSNGGWTSVPFSNGVYNIAVPNDSFRVYVFAYSGVNFTKVVEYEAGGPPSIISSHLSGNYLYFNLTNLNYDSTYYVSVYASTYVNGANLTSEMTYHMYASITQNAPTIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q978W2</accession>
    <name>Q978W2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Electron transfer flavoprotein beta-subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1344033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760277.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978W2"/>
    <dbReference type="STRING" id="273116.gene:9382110"/>
    <dbReference type="PaxDb" id="273116-14325542"/>
    <dbReference type="GeneID" id="1441420"/>
    <dbReference type="KEGG" id="tvo:TVG1344033"/>
    <dbReference type="eggNOG" id="arCOG00446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_2_1_2"/>
    <dbReference type="PhylomeDB" id="Q978W2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF17">
        <property type="entry name" value="PROTEIN FIXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="265" mass="28650" checksum="CCA54EED42FD5446" modified="2001-10-01" version="1">MLNIVVMIKQVPDSSEVEIDPVKMTLNRTKARNVVNASDLNALEYALQIKDKVGATITVISMGPPMADSAIIECMARGADRGILITDRAFAGADTYPTGLTLAATISKIGNVDIIFGGDETTDSSTGHVGPGVAEFLGIDQITYAKTVDYEDGYVVATRELEDGDEIVKVKPPVLVTVLLNSNLPRHQSLRKKIDALRKGVESWGVNELGLKPEWVGLRGSPTIVRSMKTIKEIEHMKKKIDIDHLGDMVKELVDKGLIKAGGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q978X2</accession>
    <name>Q978X2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Molybdopterin-guanine dinucleotide biosynthesis protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1334636</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760276.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978X2"/>
    <dbReference type="STRING" id="273116.gene:9382100"/>
    <dbReference type="PaxDb" id="273116-14325532"/>
    <dbReference type="GeneID" id="1441410"/>
    <dbReference type="KEGG" id="tvo:TVG1334636"/>
    <dbReference type="eggNOG" id="arCOG01872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1493045_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978X2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF81">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="186" mass="21045" checksum="27018422EA3837B1" modified="2001-10-01" version="1">MEVKMPFVVFVKRSVRFPGKHSYMIGGKSLLEIIVSKLSNIGKVYVFTKDKDIICNGCSIEFDTTNGIITDSVLAAIDKFGTFFAVAGDMPLIKPELVSKMLSQYKGSPLFPRHMDGMLEPLFGIYTEDLYNALKEYLDKGGESLHAFLLTQPLQYYDIGSDDEVNFININYKSDIEKYRDLLVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q978X4</accession>
    <name>Q978X4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1330971 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1330971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978X4"/>
    <dbReference type="STRING" id="273116.gene:9382097"/>
    <dbReference type="PaxDb" id="273116-14325529"/>
    <dbReference type="KEGG" id="tvo:TVG1330971"/>
    <dbReference type="eggNOG" id="arCOG06955">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010201_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="140" mass="15707" checksum="5B487167E85074CC" modified="2001-10-01" version="1">MAIAIVGAVTIVVDIYLGLVDIIIGIILIIYGLNLNSLLFTKSDSKVEGKVKYEWVVKEGISRIESGRLSCDRSKFISTVEKAMEAIYASDRLPEFGVEALYLYFTSRDKAQKIYEQMRTEGLDVDIQEEKIGSTIKISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Y1</accession>
    <name>Q978Y1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">propanoyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.176</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Propanoyl-CoA C-acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1323003</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.176" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917518.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Y1"/>
    <dbReference type="STRING" id="273116.gene:9382090"/>
    <dbReference type="PaxDb" id="273116-14325522"/>
    <dbReference type="DNASU" id="1441400"/>
    <dbReference type="GeneID" id="1441400"/>
    <dbReference type="KEGG" id="tvo:TVG1323003"/>
    <dbReference type="eggNOG" id="arCOG01280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="OrthoDB" id="167534at2157"/>
    <dbReference type="PhylomeDB" id="Q978Y1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004982">
        <property type="entry name" value="PRK06365.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF1">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="8">
        <location>
            <begin position="30"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="9">
        <location>
            <begin position="282"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="420" mass="45880" checksum="E929077FED4F68A3" modified="2001-10-01" version="1">MKSKGRDVYMVAGGVTKFSKASPDMDFRLRVKKAFDYAMNDAGLTLADIDGSVASYFSDHFQRQLMAGIMVQDYLGLVPKPSKRIEGGGATGGLAFQAGYEEIASGRMDAVVVYGFETMSHVNTWKGNEFIALASDTNFDYPIGGFYTGYYAMMAVRHMHEFGTTVEQMAKVSVKNHGNAIHNPYAQSPMKITVEDVRKAPMVSYPLTRLDVCAMSDGAAVAILASEDKAFEITDHPVLVKAIGTGTDTMRLSDRPFGEVPLLPNESPSDYKGLKYPGVHSFRAGRMAAKEAYEKAEITDPINDLDLIELHDAYTSSEIQTYEDLGLCKYGEGGQFIDEGKPELKGKIPVNPSGGLLAAGHPVGATGIMQAVFMFWQLQHTIKKHFHDDYLQVPNAKRGLIHSHAGTGTYVTVTIMEAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Z0</accession>
    <name>Q978Z0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1314741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Z0"/>
    <dbReference type="STRING" id="273116.gene:9382080"/>
    <dbReference type="PaxDb" id="273116-14325513"/>
    <dbReference type="DNASU" id="1441391"/>
    <dbReference type="KEGG" id="tvo:TVG1314741"/>
    <dbReference type="eggNOG" id="arCOG00731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153620_2_1_2"/>
    <dbReference type="PhylomeDB" id="Q978Z0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="124" mass="14951" checksum="275F5D67988581EE" modified="2001-10-01" version="1">MCKELYQRISKIYNCFLYIMPLVDSDYSGEYKRLIWWLFYATRGGDMRRKIIDLIRNNPSNQHQISRVLGVNYRTVEHHLHILEQNNIVYGMGDKYGRTFFISQSFLDKLPIYDEFKNKVEKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q979G3</accession>
    <name>Q979G3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1228061 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1228061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979G3"/>
    <dbReference type="SMR" id="Q979G3"/>
    <dbReference type="STRING" id="273116.gene:9381999"/>
    <dbReference type="PaxDb" id="273116-14325436"/>
    <dbReference type="DNASU" id="1441313"/>
    <dbReference type="KEGG" id="tvo:TVG1228061"/>
    <dbReference type="eggNOG" id="arCOG07370">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_961790_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="289" mass="33501" checksum="4CF7882A2133E1C5" modified="2001-10-01" version="1">MMNDYYDLLDRVKILIKLGRYKEALRIIDEVIEDSPDVSFLYYKISILFTCRRFEEAKSTLNTAISIFPGSTVFRIWKMVLALETKDVLSMAQINFNDNSVASSQLNAPFQSMDFNDNYAYTFSKSVRDLIMKLGKSAIYCDLQQLKEKILTERISTETINGLYTDIMDYRNRDIVELSSYGRAFYFYSIGRIGDSFIEISSLPDHEVFKAFILGKYSDFLKQTYEIADPYTRAVRFHILRLRELSLKEIETASYIGMDELVMSKRIIEFNGSSLSTQVVVNKVPESHL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-11" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q979G5</accession>
    <name>PYRK_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dihydroorotate oxidase B, electron transfer subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrK</name>
        <name type="ordered locus">TV1196</name>
        <name type="ORF">TVG1225518</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD(+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PyrK family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917429.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979G5"/>
    <dbReference type="SMR" id="Q979G5"/>
    <dbReference type="STRING" id="273116.gene:9381997"/>
    <dbReference type="PaxDb" id="273116-14325434"/>
    <dbReference type="DNASU" id="1441311"/>
    <dbReference type="GeneID" id="1441311"/>
    <dbReference type="KEGG" id="tvo:TVG1225518"/>
    <dbReference type="eggNOG" id="arCOG02199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_1_1_2"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="PhylomeDB" id="Q979G5"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06220">
        <property type="entry name" value="DHOD_e_trans_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.240.10">
        <property type="entry name" value="Dihydroorotate dehydrogenase, electron transfer subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01211">
        <property type="entry name" value="DHODB_Fe_S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037117">
        <property type="entry name" value="Dihydroorotate_DH_ele_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023455">
        <property type="entry name" value="Dihydroorotate_DHASE_ETsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050353">
        <property type="entry name" value="PyrK_electron_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000796">
        <property type="entry name" value="PRK00054.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513:SF3">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000148384" description="Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit">
        <location>
            <begin position="1"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01211"/>
        </source>
    </evidence>
    <sequence length="250" mass="28121" checksum="1D918B9CE93CC451" modified="2001-10-01" version="1">MINLKIEENVKESQNVNTLYFNWDQDIRPGQFAMIWIPGYGEIPMSFSGLGRRKSITVKAYGSASRKLTELKAGDDLFLRGPYGNGFDVREGRKLLVGGGSGIVSLYPIADQETDALISAKTRDELIFKDRFPESRRFIATDDGSDGFHGFAHELLQRINIDSYEAIYVCGPEQMMYKVLQVLKGRKVFVQFSLERTMKCGIGICDSCSLGGKQVCKEGPVFNIEELIGNPEFGVYRTTYSGRRIRLNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q979I5</accession>
    <name>Q979I5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1206700</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917409.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979I5"/>
    <dbReference type="STRING" id="273116.gene:9381977"/>
    <dbReference type="PaxDb" id="273116-14325414"/>
    <dbReference type="GeneID" id="1441291"/>
    <dbReference type="KEGG" id="tvo:TVG1206700"/>
    <dbReference type="eggNOG" id="arCOG03176">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_3_0_2"/>
    <dbReference type="OrthoDB" id="53209at2157"/>
    <dbReference type="PhylomeDB" id="Q979I5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="203" mass="21676" checksum="ADB95E3966CBDB80" modified="2001-10-01" version="1">MKLESNAFEKAFSSDPAPLGLAGFAFTTFLLSFINAGLIPASGVNVVVPLAIAYGGLAQLITGSWEMRRGNNFGFTAFTSYGSFWIFYALMVILADLHIINALPAVAVGWALILWGIFTLYMWGGAMMASMSLNLTFLFLWLTFVVLGLGAIYSSVALTHAGGYLGILSAFFAAYTSFAIIINSMKPGTIPVGPGMFHKKAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q979K4</accession>
    <name>Q979K4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1186360 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1186360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917391.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979K4"/>
    <dbReference type="STRING" id="273116.gene:9381957"/>
    <dbReference type="PaxDb" id="273116-14325395"/>
    <dbReference type="GeneID" id="1441273"/>
    <dbReference type="KEGG" id="tvo:TVG1186360"/>
    <dbReference type="HOGENOM" id="CLU_1507467_0_0_2"/>
    <dbReference type="OrthoDB" id="382915at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="178" mass="20471" checksum="387570D0BE190C44" modified="2001-10-01" version="1">MKLEEFLKTIVKKRMYPEIEGGIANGFPVMTTIALFAYSEMIGGLWRIVNGETEEVVFGPGQSNKNFASFLSAAGKDYTKLNSKNTYRIIRGGLIHRYFIRIRSTIEMDTSDPKAIKDYGLNCAVKFHGDEYVYFNVNQYFKDMKKTVDKILKTAKKRNRLEVEIATHIDETQYLILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q979L3</accession>
    <name>Q979L3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1177590 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1177590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60290.1"/>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979L3"/>
    <dbReference type="SMR" id="Q979L3"/>
    <dbReference type="STRING" id="273116.gene:9381947"/>
    <dbReference type="PaxDb" id="273116-14325386"/>
    <dbReference type="GeneID" id="1441264"/>
    <dbReference type="KEGG" id="tvo:TVG1177590"/>
    <dbReference type="eggNOG" id="arCOG01877">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_971877_0_0_2"/>
    <dbReference type="OrthoDB" id="33991at2157"/>
    <dbReference type="PhylomeDB" id="Q979L3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005029">
        <property type="entry name" value="PRK06438.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="293" mass="33891" checksum="57807A439E4C5C52" modified="2001-10-01" version="1">MVVKSLVYGIYPRSERLRKAYNRWERGLVGNEEIRRTISEEKDRFYEYVKSVGISEFTDPLFNWHDIFRPIALSVSGVTLGPLKRYLETNTFYREPEISGHITLQRKLDTFDLLEENPPLPLFALAKGSSIFLPTPSSFYKMSSVKANIGAEELYKDLVNVYSDIIDIFKPRSLVLYEVFPYNGDNLSYLESLNQKISVHLLTKGKISEKNFNGIKHKFESIISDDHPEFLKNYAKYYGFKIIDVNKTKVEDPNADYGIENYEDAIVTSNDYFDFLPREIADIKVEVLGKVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q979L6</accession>
    <name>Q979L6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1170798</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917378.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979L6"/>
    <dbReference type="STRING" id="273116.gene:9381943"/>
    <dbReference type="PaxDb" id="273116-14325382"/>
    <dbReference type="GeneID" id="1441260"/>
    <dbReference type="KEGG" id="tvo:TVG1170798"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="PhylomeDB" id="Q979L6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01158">
        <property type="entry name" value="SCAD_SBCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="123"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="229"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="384" mass="42303" checksum="2061FACE619212E9" modified="2001-10-01" version="1">MANFGVSPEEEMVLTYVKKFAQEELKPRAKEIDAKMEVPREIIDRMKQLGFFATYIPKEYGGLGMSFPFLIRAIEEISKACPSTALVLDGALTLFAEPIIMFGSEDLKKRYLTRVASGSVGGLAITEPGAGSDAAGISTTAEKRGDKYIINGDKIFISNGRISDFFVFDAVTDPGKRHKGITAFVVDANTPGLKISRDIHKMGIRGSSTVELVFEDMEVPAENIVGELNGGFKVIMETLDAGRIGIAAQALGIAEEAFAEAMDYIKQRKQFGTTISSFEGIQFMIADMATEIEAARYLTYVAAEKWQHKEDTIEISAMAKLKASDVAVRVTTDALQLFGGYGYTVDLDAERHMRDSKITQIYEGTNQIQRLVIAKELLKKTNYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q979M0</accession>
    <name>Q979M0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase I chain A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1166270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760266.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979M0"/>
    <dbReference type="STRING" id="273116.gene:9381939"/>
    <dbReference type="PaxDb" id="273116-14325378"/>
    <dbReference type="GeneID" id="1441256"/>
    <dbReference type="KEGG" id="tvo:TVG1166270"/>
    <dbReference type="eggNOG" id="arCOG01557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1792235_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030964">
        <property type="term" value="C:NADH dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1610">
        <property type="entry name" value="NADH:ubiquinone/plastoquinone oxidoreductase, chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000440">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038430">
        <property type="entry name" value="NDAH_ubi_oxred_su3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005022">
        <property type="entry name" value="PRK06432.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058:SF9">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00507">
        <property type="entry name" value="Oxidored_q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="144" mass="16681" checksum="6E5EBADDE6DAA1BC" modified="2001-10-01" version="1">MLDGYIPLLIIIVVLPLALIFGFKILPALGANRYGDNKISLRTEDILKKAAYEDTPPGEDQRYLEPYESGEVAREIWGRPSINQYYIVVLLFVVFDVDMLLLFPWAYDFKSLGLIPFIETLIFLAMPLFAVYYAFKLGYMRWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q979M7</accession>
    <name>Q979M7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1162330 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1162330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917367.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979M7"/>
    <dbReference type="STRING" id="273116.gene:9381932"/>
    <dbReference type="PaxDb" id="273116-14325371"/>
    <dbReference type="GeneID" id="1441249"/>
    <dbReference type="KEGG" id="tvo:TVG1162330"/>
    <dbReference type="eggNOG" id="arCOG05181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2713070_0_0_2"/>
    <dbReference type="OrthoDB" id="124473at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="entry name" value="NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005030">
        <property type="entry name" value="PRK06439.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="72" mass="7770" checksum="3C6455D5C35D80C6" modified="2001-10-01" version="1">MNKYLALVSAALFFIATAIPVIVMPGTFVPVSQDISLIGFSFFNVYIVPFELLSVIIVGAVIGVMYVARGEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-08-16" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q979N9</accession>
    <name>MDH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Malate dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">mdh</name>
        <name type="ordered locus">TV1121</name>
        <name type="ORF">TVG1151701</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of malate to oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LDH/MDH superfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917355.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979N9"/>
    <dbReference type="SMR" id="Q979N9"/>
    <dbReference type="STRING" id="273116.gene:9381920"/>
    <dbReference type="PaxDb" id="273116-14325359"/>
    <dbReference type="GeneID" id="1441237"/>
    <dbReference type="KEGG" id="tvo:TVG1151701"/>
    <dbReference type="eggNOG" id="arCOG00246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_2_1_2"/>
    <dbReference type="OrthoDB" id="2596at2157"/>
    <dbReference type="PhylomeDB" id="Q979N9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01339">
        <property type="entry name" value="LDH-like_MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000018">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000004">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00487">
        <property type="entry name" value="Malate_dehydrog_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011275">
        <property type="entry name" value="Malate_DH_type3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01763">
        <property type="entry name" value="MalateDH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004863">
        <property type="entry name" value="PRK06223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF16">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_0000113497" description="Malate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="124"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O08349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P61889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="325" mass="35390" checksum="0B6012E92B6F9433" modified="2001-10-01" version="1">MARKKISVIGAGNVGATVAQFLATKELGDVYLFDVVDGIPEGKALDIQEGAPHWGYDLDVVGFSTSDSSNYKNMEGSDVIVVTAGMARKPGMSREDLFDKNVEIIADVSKNIKKYSPDSIIVVVSNPADIMAYALQKISGVDPQRIMGLGGSLDSSRFRTFLAKELDVSVEDVNAFVIGGHGDDMVPFIRYSSVAGIPIEKLLPKEKIDAIVKRTRFGGGEIVNYLKAGSAYYAPGISITAMVESVIKDKKRVIPCAAYITGKHAEHYGINNKFIGVPIKIGERGVEEIYDIDFLPEELELWKKSVASVEASSKNVDEWLKKHPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q979P5</accession>
    <name>Q979P5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">3-hydroxybutyryl-coa dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1148597</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917349.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979P5"/>
    <dbReference type="STRING" id="273116.gene:9381914"/>
    <dbReference type="PaxDb" id="273116-14325353"/>
    <dbReference type="GeneID" id="1441231"/>
    <dbReference type="KEGG" id="tvo:TVG1148597"/>
    <dbReference type="eggNOG" id="arCOG00250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_2"/>
    <dbReference type="OrthoDB" id="51300at2157"/>
    <dbReference type="PhylomeDB" id="Q979P5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022694">
        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000105">
        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="6">
        <location>
            <begin position="5"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="5">
        <location>
            <begin position="219"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="3">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="314" mass="35200" checksum="283A5174B141C21A" modified="2001-10-01" version="1">MSIKKVGIVGAGTMGSAIAEVFAYNGYNVILKDQTIELAERGRENAKRIIEGYEKISNEKVKKEITKIEGYGIKLTEEQKSSITKSLSVNVPVKEVEERIIVTSKNDEMSDCDLIIEAAFEDQAVKNQIFRELSDFSNNAIIASNTSSLSITEMSKNLKKPENGIIMHFFNPPYLLPLVEVVPSLFTSNETLTAVMDLLHGMKNHRENMVPVLAKEREGFIVNRLLIPFINEASKMLDYGIATEKDIDIAMKKGAGFPMGPFELADMIGIDVVKDVMNVFEKTYGDQYKTSQLIRRMSEAGKLGRKTKEGFYKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q979R3</accession>
    <name>TRM56_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>tRNA (cytidine(56)-2'-O)-methyltransferase</fullName>
            <ecNumber>2.1.1.206</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>tRNA ribose 2'-O-methyltransferase aTrm56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV1097</name>
        <name type="ORF">TVG1129508</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(56) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42968"/>
            <dbReference type="Rhea" id="RHEA-COMP:10308"/>
            <dbReference type="Rhea" id="RHEA-COMP:10309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.206"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aTrm56 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.206"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917329.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979R3"/>
    <dbReference type="SMR" id="Q979R3"/>
    <dbReference type="STRING" id="273116.gene:9381894"/>
    <dbReference type="PaxDb" id="273116-14325335"/>
    <dbReference type="DNASU" id="1441211"/>
    <dbReference type="GeneID" id="1441211"/>
    <dbReference type="KEGG" id="tvo:TVG1129508"/>
    <dbReference type="eggNOG" id="arCOG01857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_817897_0_0_2"/>
    <dbReference type="OrthoDB" id="14397at2157"/>
    <dbReference type="PhylomeDB" id="Q979R3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
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        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
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        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
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        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK12703.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197">
        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
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    </dbReference>
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        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="alpha/beta knot"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000365327" description="tRNA (cytidine(56)-2'-O)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="188"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="105"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="338" mass="38017" checksum="14E4AE364DD97353" modified="2001-10-01" version="1">MIAVLRINHRPFRDKRITTHVALTARAFGASSILVDEKDETLEQTINKVVENFGGNFFIKSGVDWKREFRNFKGIRVHLTMYGRPLEDVVKEIRESKKDVMILVGSEKVPFEAYEIADYNVSVTNQPISEVSALAIFLDRFYDGEELNWRFTGKINVYPSERGKKVKIIPDEQGCLDLLYKYGASDYLINHVKSVKELAVAIAKKTNADINLVTAGALLHDIGRTQVQGITHAVVGADILRREGIDDRVVSIVEKHIGAGIQSEEAVKLGLPPDNYVPETIEEMIVAHADNLFAGDKRLKLQQVVDNYRKKGLEDAAERIAKLHKFLSTVIGQDMDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q979R5</accession>
    <name>Q979R5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1126727</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeosine synthase type 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979R5"/>
    <dbReference type="STRING" id="273116.gene:9381892"/>
    <dbReference type="PaxDb" id="273116-14325333"/>
    <dbReference type="KEGG" id="tvo:TVG1126727"/>
    <dbReference type="eggNOG" id="arCOG00990">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029831_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q979R5"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
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    </dbReference>
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        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF5591"/>
    </dbReference>
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        <property type="entry name" value="PUA"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="491"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="555" mass="64368" checksum="3F9B75D122B21C85" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q979R8</accession>
    <name>Q979R8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1122712 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1122712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979R8"/>
    <dbReference type="STRING" id="273116.gene:9381889"/>
    <dbReference type="PaxDb" id="273116-14325330"/>
    <dbReference type="KEGG" id="tvo:TVG1122712"/>
    <dbReference type="eggNOG" id="arCOG07743">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631734_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="170" mass="20252" checksum="425E95EB71245566" modified="2001-10-01" version="1">MLRSYTSRRRGLRTSILNLFFSNLIAYPLLIFRFLIPVVLLFIMVFLVKRYSKKAVPDDYPSEQQKRESFQTPNDYISTEYPHVKNNDIDYYLRLIERELDYADNILSSYKHLLKMYRKYDAMSHSSITRRRKRAAVKKEELFEEIVRIYARLGKLKYDIVVAGGVGGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q979S0</accession>
    <name>Q979S0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG1120468 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1120468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979S0"/>
    <dbReference type="STRING" id="273116.gene:9381887"/>
    <dbReference type="PaxDb" id="273116-14325328"/>
    <dbReference type="KEGG" id="tvo:TVG1120468"/>
    <dbReference type="eggNOG" id="arCOG02742">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_643440_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q979S0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33608">
        <property type="entry name" value="BLL2464 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33608:SF6">
        <property type="entry name" value="BLL2464 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="DUF58" evidence="2">
        <location>
            <begin position="194"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="426" mass="49093" checksum="63967CF711116F4A" modified="2001-10-01" version="1">MIISATIYNIFESIILGYKYYIIFSFILFFVFTSDILIFNLSSRRYLPNVEVDLRVGSEKVRKYSKHNVFVSFLNKNNGVVSFHYYVYTSDTFKLTGDYENFLTLGPYERVEKSFVIEPTTIGNYVLGPVSIYTEDGMKLAIERLEVSKSVDLKVAPSLSETMAARSERLSNMLYYTGVHYNKKAGEGYDFLSLREYYPGDEIRYVAWSRFGRTRGDDLYVKQMEEERIIDVLFVVDYGIGMNHGHDGIRMFDTVISDVLKTSYKIRKNQDGVGFMISSSAFEYYIKPSRTARPVEDLEKVVSGIRPAGKFDFVNIVQDTKKKVKKSTLIMIISSFSSTDNIMRGLEDLKGYKAMFFVLNPYEFIVDGTENMTLRYTLFTRQYAISKAIASVIKSRGFRSDVCGRKDLYRKLVSAYIYAKMTNMGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q979S6</accession>
    <name>Q979S6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Farnesyl pyrophosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1113858</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917315.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979S6"/>
    <dbReference type="STRING" id="273116.gene:9381880"/>
    <dbReference type="PaxDb" id="273116-14325322"/>
    <dbReference type="GeneID" id="1441197"/>
    <dbReference type="KEGG" id="tvo:TVG1113858"/>
    <dbReference type="eggNOG" id="arCOG01726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_3_2"/>
    <dbReference type="OrthoDB" id="26738at2157"/>
    <dbReference type="PhylomeDB" id="Q979S6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="326" mass="36984" checksum="634F034EB6FBED1A" modified="2001-10-01" version="1">MKSFYEWELALHSKIEEVNERLFEVVKSEEPDLDRMAKYTIEAGGKRFRPLLTILAFESSTDDPYFKILDLAAGYELIHTASLIHDDIIDDSPMRRGRPTLSYKEGINNAIVVADYLFAKAYELGSRYGPLVSKVMADASSRLAEGQILEAVNLGNLDITEDTYYKIIENKTAHFFEACALGATIVAEADRPTREILSNFAFNLGMAFQVTDDILDIVGDDRYTGKPTFVDIKHDALTLPIIYALRNSNDNDRRAIIDMINGRREINKSLLKDIFLKSGSIDYSFSVAKQYITKSMDYLKKAKKSPDIDLLMELAFIVIDRIEIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q979V3</accession>
    <name>Q979V3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1082508 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1082508</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917286.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979V3"/>
    <dbReference type="STRING" id="273116.gene:9381851"/>
    <dbReference type="PaxDb" id="273116-14325295"/>
    <dbReference type="GeneID" id="1441168"/>
    <dbReference type="KEGG" id="tvo:TVG1082508"/>
    <dbReference type="HOGENOM" id="CLU_1032983_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="269" mass="30415" checksum="AF70A9B3A9329F59" modified="2001-10-01" version="1">MEVNGHINYEFIVNEERPIFLHKSTSDDFVNMLGRVLPRRVLLDEFLFQDRSIPPELAYAIEDHKEIIRIVERGKGISIELLGNPYIFSALFQIYFSGKDVQTFPYGKLWEDAVKEAADVFDRVIVVDDISEDPKESIRTAFSKGYHFITPELPNSNSFYNSVIVSRSVRKTHNRYSIKANGNFDPLVLDGFISAFGDPEEIRIVIDGDPSVEADNIDILSGMMNVSGIKIGRKPFPSPYLTKFYKSGKLKVSSGRIPGNTVLSLDVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q979W0</accession>
    <name>Q979W0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">D-glyceraldehyde dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="8">1.2.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">TVG1076124</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">NADP-dependent dehydrogenase of the nED (non-phosphorylated Entner-Doudoroff) pathway with highest activity towards glyceraldehydes (e.g. D,L-glyceraldehyde and D-glyceraldehyde), to a lesser extent towards D,L-glyceraldehyde-3-phosphate and glycolaldehyde, but no activity towards aliphatic or aromatic aldehydes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>D-glyceraldehyde + NADP(+) + H2O = (R)-glycerate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:40163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16659"/>
            <dbReference type="ChEBI" id="CHEBI:17378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.89"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; glycolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer. Dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the aldehyde dehydrogenase family. Glyceraldehyde dehydrogenase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.89" evidence="8"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917279.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979W0"/>
    <dbReference type="STRING" id="273116.gene:9381844"/>
    <dbReference type="PaxDb" id="273116-14325288"/>
    <dbReference type="GeneID" id="1441161"/>
    <dbReference type="KEGG" id="tvo:TVG1076124"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_5_1_2"/>
    <dbReference type="OrthoDB" id="6342at2157"/>
    <dbReference type="PhylomeDB" id="Q979W0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043796">
        <property type="term" value="F:glyceraldehyde dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009255">
        <property type="term" value="P:Entner-Doudoroff pathway through 6-phosphogluconate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07088">
        <property type="entry name" value="ALDH_LactADH-AldA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000009">
        <property type="entry name" value="Aldehyde dehydrogenase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000022">
        <property type="entry name" value="Aldehyde dehydrogenase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053507">
        <property type="entry name" value="NADP-Glyceraldehyde_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040792">
        <property type="entry name" value="glyc_ald_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="11"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00050427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00055147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAB60192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="493" mass="54801" checksum="EBEC6E8BB292CD54" modified="2001-10-01" version="1">MDGKLYIDGQWVDSSNGDTLTKYNPVTGEVIGKFAAASRDDVDRAFDAAEDAFQKWMDLGSVERSKIIYKAKELIEENRKELENIIIQENGKPVKEAAEEVDGVLDQMQYYAEWARKLNGEVVEGTTNHRKIFQYKVPYGTVVALTPWNFPAGMVARKLAPALLTGNTVILKPSSDTPGSAEWIVKKFIQAGVPKGALNYITGRGSVIGDYIVEHKKVALITMTGSTSTGQRIMQKASANMAKLMLELGGKAPFMVWKDADINNALKTLLWAKYWNAGQSCIAAERLYVHEDIYDTFINKFVELSKKIVVGDPSRADMGPLINTTALKGTEEVVEEAKESGARILYGGSKPDLSGPYKNGYFFLPTIIDNVDQKSKIFQEEIFAPVIGARKVSDVEEMYSLANDSKYGLASYLFTKDPNLIFEAAERIRFGELYINMPGPEASQGYHTGFRLTGQAGEGSRYGILEYLKIKNIYVDYSGKPLQINTVRDDLFQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q979W3</accession>
    <name>FOLD_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein FolD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methylenetetrahydrofolate dehydrogenase</fullName>
                <ecNumber evidence="1">1.5.1.5</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methenyltetrahydrofolate cyclohydrolase</fullName>
                <ecNumber evidence="1">3.5.4.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">folD</name>
        <name type="ordered locus">TV1047</name>
        <name type="ORF">TVG1072456</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="1"/>
    <dbReference type="EC" id="3.5.4.9" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917276.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979W3"/>
    <dbReference type="SMR" id="Q979W3"/>
    <dbReference type="STRING" id="273116.gene:9381841"/>
    <dbReference type="PaxDb" id="273116-14325285"/>
    <dbReference type="GeneID" id="1441158"/>
    <dbReference type="KEGG" id="tvo:TVG1072456"/>
    <dbReference type="eggNOG" id="arCOG04538">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034045_2_1_2"/>
    <dbReference type="OrthoDB" id="9455at2157"/>
    <dbReference type="PhylomeDB" id="Q979W3"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054993">
        <property type="entry name" value="FolD_Thplmales"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041156">
        <property type="entry name" value="FolD_Thplmales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF5">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000268590" description="Bifunctional protein FolD">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="159"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01576"/>
        </source>
    </evidence>
    <sequence length="281" mass="31006" checksum="A0DCBF88CB6ABE3A" modified="2001-10-01" version="1">MAVKLLKGEEIAEKKAEELKERIEKMGVSPRLVLLQVGNYSAATIYARAKIKRGKKIGADVVLEKYEDLTKQELVRRIEELSADKDVNGIMVENPLPKTIDYYDIVKSIPYYKDVDALSPYNQGSIAINREFLVPATAMAVVDILKYYGYEKTTATIINRSPVVGRPLSMMMLNRDYTVSICHSKTPDIRSIAKASKIIVVAVGRPGFLDDTYVTESSVVIDVGINYVQDKVIGDIDFDKVSEKVEAVTPVPGGVGPITATNILSNLVKAAEYQSNTNIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Y2</accession>
    <name>Q979Y2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Adenylosuccinate lyase</fullName>
            <ecNumber evidence="5 12">4.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">TVG1053296</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes two reactions in de novo purine nucleotide biosynthesis. Catalyzes the breakdown of 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR or 2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate) to 5-aminoimidazole-4-carboxamide ribotide (AICAR or 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) and fumarate, and of adenylosuccinate (ADS or N(6)-(1,2-dicarboxyethyl)-AMP) to adenosine monophosphate (AMP) and fumarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="5 12"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917257.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Y2"/>
    <dbReference type="STRING" id="273116.gene:9381821"/>
    <dbReference type="PaxDb" id="273116-14325266"/>
    <dbReference type="GeneID" id="1441139"/>
    <dbReference type="KEGG" id="tvo:TVG1053296"/>
    <dbReference type="eggNOG" id="arCOG01747">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_0_1_2"/>
    <dbReference type="OrthoDB" id="7033at2157"/>
    <dbReference type="PhylomeDB" id="Q979Y2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01360">
        <property type="entry name" value="Adenylsuccinate_lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylosuccinate lyase C-terminal" evidence="13">
        <location>
            <begin position="360"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB60170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="448" mass="50841" checksum="F6485B18DDD70237" modified="2001-10-01" version="1">MVVSPLEYRYGREQIKRIFDDENRLYYMLKVEAAISKAEAEYGIIPPEAAEDIGKVVADRIVQLQRVREIEAEIKHDVMAMVKALSEKCKVGASYVHYGVTSNDVNDTATALQMHDFFSFLKQDIEDIMETLIELTLRYKSSPMMGRTHGQHASPITIGLKFAVYLSEFSRHYDRMIEMSKRAFAGKVLGPVGTGAALGPKALDIQNRVMEILGIYAEEGPTQIVNRDRYIEYLSVINGISVTLEKIGTEIRNLQRPEIDEMSEYFDVERQVGSSSMPSKVNPINSENVVSLSRLIRSMIIPEYEAGVTWHERDLTNSALERFTIPYSSILVDYILVKMNSILSSLIIKEDKIRKNLEMDDSIFSESLVAALTQAGMPRQDAHEFIRRCSMKARSEGKTLKHVVVEEGITNYIRRDELDRLMDPANFIGSAPEICDIVVNNARRRLIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-10-14" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Y7</accession>
    <name>GGR_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Digeranylgeranylglycerophospholipid reductase</fullName>
            <shortName evidence="1">DGGGPL reductase</shortName>
            <ecNumber evidence="1">1.3.1.101</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2,3-bis-O-geranylgeranylglyceryl phosphate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Geranylgeranyl reductase</fullName>
            <shortName evidence="1">GGR</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV1023</name>
        <name type="ORF">TVG1046096</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains. Is also probably able to reduce double bonds of geranyl groups in CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol and archaetidylserine, thus acting at various stages in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 NADP(+) = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 NADPH + 8 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36035"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:73125"/>
            <dbReference type="EC" id="1.3.1.101"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:36037"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 NAD(+) = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 NADH + 8 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36039"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:73125"/>
            <dbReference type="EC" id="1.3.1.101"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:36041"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid + 8 A = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64376"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:138139"/>
            <dbReference type="ChEBI" id="CHEBI:138140"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:64378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + 8 AH2 = CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 8 A</text>
            <dbReference type="Rhea" id="RHEA:84207"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="ChEBI" id="CHEBI:74004"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:84208"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>archaetidylserine + 8 AH2 = 2,3-bis-O-phytanyl-sn-glycero-3-phospho-L-serine + 8 A</text>
            <dbReference type="Rhea" id="RHEA:84215"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:71517"/>
            <dbReference type="ChEBI" id="CHEBI:74853"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:84216"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reduction reaction proceeds via syn addition of hydrogen for double bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the geranylgeranyl reductase family. DGGGPL reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.101" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917251.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Y7"/>
    <dbReference type="SMR" id="Q979Y7"/>
    <dbReference type="STRING" id="273116.gene:9381816"/>
    <dbReference type="PaxDb" id="273116-14325261"/>
    <dbReference type="DNASU" id="1441133"/>
    <dbReference type="GeneID" id="1441133"/>
    <dbReference type="KEGG" id="tvo:TVG1046096"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_0_0_2"/>
    <dbReference type="OrthoDB" id="6062at2157"/>
    <dbReference type="PhylomeDB" id="Q979Y7"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045550">
        <property type="term" value="F:geranylgeranyl reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
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</entry>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
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        </source>
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            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
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    <sequence length="234" mass="26519" checksum="08F859756D9BEE2F" modified="2001-10-01" version="1">MERKFIKTTFGNLSYLERPGEYPLIFLHGLGGSSNNWLRLDRFLAPRFRLIFFDLLGHGQSDKPKIEYTVEVQAKAIHEAINSLGISRFSIAGNSYGGWVSLYLSIHIAKPDKLILIDSAGINKTVGESGEEMIESFVQKVMGVEDGNDEEVIRNIIRNNAKHEWKIKEEDLKNLSVPTIIIWGTADNILSIDYGRKLHDLIQNSKLYEIPAAKHTPQRTHPQILAKILNENIV</sequence>
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                <person name="Yamamoto Y."/>
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                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    </evidence>
    <sequence length="637" mass="73052" checksum="2D78ED07C5FB27E6" modified="2001-10-01" version="1"> </sequence>
</entry>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
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                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
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            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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    </evidence>
    <sequence length="295" mass="33135" checksum="FBEA52BB0BF10243" modified="2001-10-01" version="1">MNKVSVEKISKSYGKFRALDNVSFEVEGNGCLVLLGPNGAGKTTLMKTMTNIIRPTSGNVKINDINVRENPKKALMNVGSLIEQPEFYSYITGYEALMFTCMMRGLDRKTCESEVENLARLTNSKGYLTRKTREYSRGMKQRVGLASAMIGDPNIIILDEPTFGLDPSGMKEVRDLIIKLKKEKLIILSTHLLYEAQQLADWVAIINSGKLQYYGEQEKENYIEITGNVDVKKLPNSIDSVIYAGQNRIIVKKKTGARNSEIIENLIELGNTVDTFNYYSILEDLYVNQVGMTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A43</accession>
    <name>Q97A43_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
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        <name evidence="7" type="ORF">TVG0990514</name>
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            <taxon>Thermoplasmatota</taxon>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_20_3_2"/>
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    <dbReference type="Proteomes" id="UP000001017">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
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    </dbReference>
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            <begin position="21"/>
            <end position="46"/>
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            <end position="482"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAB60109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="497" mass="54025" checksum="B8073007827527FC" modified="2001-10-01" version="1">METGSDKFKHLIVNQLGLRHAISQAVALNAPAGTIVLYVAGTASIITSTFITHPFGSYAIPLILLLSLVVYALMSYSMYEFSRKLSSAGGYYTFVSRGLGNSAGFVTALSYITYEILSFTGFGILGFLGFAYAIFPSLGIAIPDPEFLWMPVAFVFIGLVSLLIYLGIKPSLRFVSYTVFIEVAFLIFTSVALIVMHHTSVNLEPFTPTPMGNDPYAIFAMMIYAIGAFVGIGGSIPIAEETKKPKRNVPLAILVTIAVLGITIILASYAQVVSWGLNRIDLFGSSSDPYPLLTIYHSAFGPLSIILFWAIIIIVLNSFFTATVSLGTNATRVIFSMAREGALPSFLSEMNRNNGTPTMAMIVLTAISIAIVIGVGAGFEFFGHFSPVNALLTSSVFLLILESPITYIVHILTNTSLYSYMKKRTKMKAKDLFKYLVIPSVSTVTLIAAIVAGIYFNLFLVDAIYASFALIAIIIISTLVMRAKFKEKLEYLGDFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A60"/>
    <dbReference type="STRING" id="273116.gene:9381742"/>
    <dbReference type="PaxDb" id="273116-14325167"/>
    <dbReference type="GeneID" id="1442029"/>
    <dbReference type="KEGG" id="tvo:TVG0975318"/>
    <dbReference type="eggNOG" id="arCOG00644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_0_2"/>
    <dbReference type="OrthoDB" id="6633at2157"/>
    <dbReference type="PhylomeDB" id="Q97A60"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF3">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT UROPORPHYRINOGEN III METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="252" mass="27261" checksum="F47188ECC5DD01D4" modified="2001-10-01" version="1">MKKFYIVGAGPGEPDLITVMGLEAIRESQVVFKDALVSDDLIEKYCTNKVQVNVGHRQGTSSDVGLKIILNYIKENANNFDVAVHLKSGDPGIFSRLSEEIRAVSTLGFDVIVVPGITSAIGVPTFYGIPLSVKDVSRHIAIVTASISEGKFNASSLGDALKYADVVVVLMGSRFLKQIARLSQSILGHSDMLIVENGTLPSAKISLLKDAENLDEHYCSKPSIIILGRKLNEILSLGGGFDEYRVYAGYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A65</accession>
    <name>Q97A65_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Precorrin-2 c20-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0971375</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054008.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A65"/>
    <dbReference type="STRING" id="273116.gene:9381737"/>
    <dbReference type="PaxDb" id="273116-14325162"/>
    <dbReference type="GeneID" id="1442024"/>
    <dbReference type="KEGG" id="tvo:TVG0971375"/>
    <dbReference type="eggNOG" id="arCOG00648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076014_2_0_2"/>
    <dbReference type="OrthoDB" id="23546at2157"/>
    <dbReference type="PhylomeDB" id="Q97A65"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006364">
        <property type="entry name" value="CobI/CbiL/CobIJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01467">
        <property type="entry name" value="cobI_cbiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43467">
        <property type="entry name" value="COBALT-PRECORRIN-2 C(20)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43467:SF2">
        <property type="entry name" value="COBALT-PRECORRIN-2 C(20)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036427">
        <property type="entry name" value="Precrrn-2_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="233" mass="25883" checksum="931AFA340729EA49" modified="2001-10-01" version="1">MNADMKLYVVGLGPGDPSLVTLKALDTVLKSDIIYVPCSGGEHRIARSILEKLFAGKNEGSLPQVKDLEFPMTRDHNYNKSFLEKNVAEIISDLAKHKILSYAVIGSPTFYSTFGKIKQMLEEKGVSIEYVPGVNSVDACSARSSINIASGDDSVLVTTYRKLLEIGSFEKYDTVVIMKVPTGSFSLERIRNLMGDGMKYIYARRCTMDSEFITEKAGNEDGDYFSLVIARRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A71</accession>
    <name>Q97A71_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidohydrolase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0965932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A71"/>
    <dbReference type="STRING" id="273116.gene:9381731"/>
    <dbReference type="MEROPS" id="M38.001"/>
    <dbReference type="PaxDb" id="273116-14325156"/>
    <dbReference type="DNASU" id="1442020"/>
    <dbReference type="KEGG" id="tvo:TVG0965932"/>
    <dbReference type="eggNOG" id="arCOG00691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009942_3_1_2"/>
    <dbReference type="PhylomeDB" id="Q97A71"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01300">
        <property type="entry name" value="YtcJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033932">
        <property type="entry name" value="YtcJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642:SF2">
        <property type="entry name" value="PROTEIN LONG AFTER FAR-RED 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase 3" evidence="1">
        <location>
            <begin position="38"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="457" mass="51446" checksum="F7A0E3494B8B9CF8" modified="2001-10-01" version="1">MISNCTVFGREGSDAIGINEGRIAFIGIDRDLRAKKRIDLSGKIVFPGFIDSHAHLLSTGLEDIRLNLYHTKSKEEVVERIAEYSKGKEKVIAYRWDESMWKPQSSYLTSSDINGIPVPVVAFRRDGHMAVANQKAMDVIGTTRKEGIFKEDDIELLEPLVKPNEAEIRDALNTAANRAISLGIVAVRDMVDISTYNEYKNIKTPLKIYKVLYSDSIFDKFGTSSQNDWGIKAFLDGSLGSRTAAHKGWDASNLKMDESKFENFAKQIWMHNLPLAVHAIGEVAVETAAKVFYRNAGRNRNSIEHFELVDDDVLDYITKTTIISSQPNFLEWAGKGGMYEDRVGGEWLYKNNPFRWMIDRGIHLAFGSDSMPIGPMYGIYYAVNSEYQRQKITLEEAVRCYSEGGSYLLGTEGYMGMLRVGYSADMAVFDEKLLKDLRGIKAAKPEITIINGKIYTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A72</accession>
    <name>Q97A72_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0965928 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0965928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917171.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A72"/>
    <dbReference type="PaxDb" id="273116-14325155"/>
    <dbReference type="GeneID" id="1442019"/>
    <dbReference type="KEGG" id="tvo:TVG0965928"/>
    <dbReference type="eggNOG" id="arCOG06055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204197_0_0_2"/>
    <dbReference type="OrthoDB" id="55755at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="115" mass="13245" checksum="C4F348C026CDD749" modified="2001-10-01" version="1">MKFEFEYLKTPEDLMISLSGSFAMAMRNVAEIYSYKGYLAYYSPSLSDGDDSMIIVSLTRGTLNPGLVDFDSETKKIEYVKRSTNPERYHFLIIEPELSTILDKAIYEFEKLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AA1</accession>
    <name>Q97AA1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0934671</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917149.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AA1"/>
    <dbReference type="STRING" id="273116.gene:9381701"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325126"/>
    <dbReference type="DNASU" id="1441997"/>
    <dbReference type="GeneID" id="1441997"/>
    <dbReference type="KEGG" id="tvo:TVG0934671"/>
    <dbReference type="eggNOG" id="arCOG01411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_812868_0_0_2"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="PhylomeDB" id="Q97AA1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="170"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="341" mass="39823" checksum="FD0454C33140A4FF" modified="2001-10-01" version="1">MINFININPVYSGIYKITRSYYESLEDKNIEAKWINIRFDARHNFSEFINPPLYHFTQLVGNGYFLNQLVNRFLKLNTGNIFSILTSPDIYVSNVKNVLYVIHDFFPVDDELSEERTEKVRIIDKIGTKNVFNYILRNDIPVITPSIFVANIARNKGYNVITPLYYKKRMSEIKRVRETVKTILSIGSNDQRKRPDLVIDFLNSLPAEYTFIRVGGDLSTLHGKEIKPKCKFEYYHNLSEDQLDALYNRADILFFPSEKEGLGLPIIEALIHRIPVLINSKLETAKELDYGMGTFIYFEDVNDIDLETVYDKRPDFDGWYKKYSEISEGQLNNLVDFIKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AA2</accession>
    <name>Q97AA2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S9 prolyl oligopeptidase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0932389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917148.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AA2"/>
    <dbReference type="STRING" id="273116.gene:9381700"/>
    <dbReference type="ESTHER" id="thevo-TV0908">
        <property type="family name" value="ACPH_Peptidase_S9"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.071"/>
    <dbReference type="PaxDb" id="273116-14325125"/>
    <dbReference type="GeneID" id="1441996"/>
    <dbReference type="KEGG" id="tvo:TVG0932389"/>
    <dbReference type="eggNOG" id="arCOG01646">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008615_2_2_2"/>
    <dbReference type="OrthoDB" id="25019at2157"/>
    <dbReference type="PhylomeDB" id="Q97AA2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF4">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69304">
        <property type="entry name" value="Tricorn protease N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="372"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="581" mass="65899" checksum="76BF1B5D26F1E5B9" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AC2</accession>
    <name>Q97AC2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0904721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AC2"/>
    <dbReference type="STRING" id="273116.gene:9381680"/>
    <dbReference type="PaxDb" id="273116-14325105"/>
    <dbReference type="KEGG" id="tvo:TVG0904721"/>
    <dbReference type="eggNOG" id="arCOG01400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080078_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AC2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase FkbM" evidence="1">
        <location>
            <begin position="116"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="293" mass="33933" checksum="3EB630F29E04728C" modified="2001-10-01" version="1">MKMGMKDMMRTIKRYKEAYRNYMGVMWNVWRGRDRIKVILQNGNSTYLNHDLAYAYSVLFTTRNDHIYNVNLEGNSIKFTYNGHNIMFEYPIGDAAGVFGFEIYAFLRVENETVIDIGANIGDSSIYFALNNAKKVIALEPYPYSYNIALKNIKNYDLQDKIILLNAGYGQDGTIKVDPDFKNPGTINLRSFTDGMDIKILSLKTLLSDYQIDKAVLKMDCEGCEYNLLKEDNDTLKKFERIEIEYHYGYEKLKDKLEEAGFTVTYTKPVKLFNKDATEHNMRMGYIYAKSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AF5</accession>
    <name>Q97AF5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0876539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053994.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AF5"/>
    <dbReference type="STRING" id="273116.gene:9381647"/>
    <dbReference type="PaxDb" id="273116-14325072"/>
    <dbReference type="GeneID" id="1441947"/>
    <dbReference type="KEGG" id="tvo:TVG0876539"/>
    <dbReference type="eggNOG" id="arCOG00701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1640066_0_0_2"/>
    <dbReference type="OrthoDB" id="57134at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08556">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211:SF1">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="161" mass="18201" checksum="7DF0457927E7F689" modified="2001-10-01" version="1">MKYSDISEVKLISGGPIPSLSEVLERYSSMNYFIEIKVEHFDNKAARLVDLVANNIYASRLIDRSIVISFSGEAIDYLTEHYPDIRSGLDFDDPGLLEDAQSKYGVLLPHYFLISQDFCRNLRKPVIPWTVDDVELANKLKKLGCKGIITNVGDRMLSAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AH3</accession>
    <name>Q97AH3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA polymerase</fullName>
            <ecNumber evidence="7">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG0859451</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="7"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AH3"/>
    <dbReference type="STRING" id="273116.gene:9381629"/>
    <dbReference type="PaxDb" id="273116-14325054"/>
    <dbReference type="KEGG" id="tvo:TVG0859451"/>
    <dbReference type="eggNOG" id="arCOG00328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_6_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AH3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00145">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="10">
        <location>
            <begin position="105"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="9">
        <location>
            <begin position="353"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="773"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="775"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="800" mass="91834" checksum="EA9640C12B134929" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AN1</accession>
    <name>Q97AN1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0782903 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0782903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917024.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AN1"/>
    <dbReference type="PaxDb" id="273116-14324995"/>
    <dbReference type="DNASU" id="1441872"/>
    <dbReference type="GeneID" id="1441872"/>
    <dbReference type="KEGG" id="tvo:TVG0782903"/>
    <dbReference type="HOGENOM" id="CLU_2802483_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="67" mass="7310" checksum="889DA37B494D478D" modified="2001-10-01" version="1">MEMKTKKAVLMSSLALSIIAFLLALILMQPNSAGYPEVPAVPTYVLFGLSAASFAVFIYEYFTATRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AQ5</accession>
    <name>Q97AQ5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CoA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0759889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AQ5"/>
    <dbReference type="STRING" id="273116.gene:9381544"/>
    <dbReference type="PaxDb" id="273116-14324970"/>
    <dbReference type="KEGG" id="tvo:TVG0759889"/>
    <dbReference type="eggNOG" id="arCOG04227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112567_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AQ5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="15"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="141" mass="16078" checksum="3373426ABB5C06CC" modified="2001-10-01" version="1">MIFEYAFMDQIVDILTHYKNVAVVGISSNPEKDSYRVAKYLMEHGFRVIPVNPNLSSWEGLKAYPSVSAIEDKVEIVDVFRKPEAVLEVVDDALKVSPKVIWMQEGVVNEEARQLAEKHGINVVMDKCMMKEHIKLANSQR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AR4</accession>
    <name>GGGPS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Geranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="1">GGGP synthase</shortName>
            <shortName evidence="1">GGGPS</shortName>
            <ecNumber evidence="1">2.5.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(S)-3-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoglycerol geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV0745</name>
        <name type="ORF">TVG0750626</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>sn-glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = sn-3-O-(geranylgeranyl)glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23404"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GGGP/HepGP synthase family. Group II subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59887" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.5.1.41" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59887.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916992.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="6NKE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.72 A"/>
        <property type="chains" value="A=1-252"/>
    </dbReference>
    <dbReference type="PDBsum" id="6NKE"/>
    <dbReference type="AlphaFoldDB" id="Q97AR4"/>
    <dbReference type="SMR" id="Q97AR4"/>
    <dbReference type="STRING" id="273116.gene:9381535"/>
    <dbReference type="PaxDb" id="273116-14324961"/>
    <dbReference type="GeneID" id="1441840"/>
    <dbReference type="KEGG" id="tvo:TVG0750626"/>
    <dbReference type="eggNOG" id="arCOG01085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068610_0_0_2"/>
    <dbReference type="OrthoDB" id="7409at2157"/>
    <dbReference type="PhylomeDB" id="Q97AR4"/>
    <dbReference type="BRENDA" id="2.5.1.41">
        <property type="organism ID" value="6326"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120536">
        <property type="term" value="F:heptaprenylglyceryl phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047294">
        <property type="term" value="F:phosphoglycerol geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02812">
        <property type="entry name" value="PcrB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00112">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039074">
        <property type="entry name" value="GGGP/HepGP_synthase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010946">
        <property type="entry name" value="GGGP_synth"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01769">
        <property type="entry name" value="GGGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003198">
        <property type="entry name" value="PRK04169.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40029">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40029:SF2">
        <property type="entry name" value="HEPTAPRENYLGLYCERYL PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000138747" description="Geranylgeranylglyceryl phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="170"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="201"/>
            <end position="202"/>
        </location>
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        <source>
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        <source>
            <dbReference type="PDB" id="6NKE"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Makino S."/>
                <person name="Higuchi S."/>
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                <person name="Watanabe K."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto Y."/>
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                <person name="Nunoshiba T."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
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                <person name="Yamazaki M."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="142" mass="15787" checksum="A9BC1BCFF704303F" modified="2001-10-01" version="1">MLKSLEGRPKVVIVSKVDRFARSLSDLLRILEFLGHNGTGFISINDPGIDTTTPNGRLLLQILGAFEEFERNMINSFTKAGREKAMNKGVKFGRPALKTRNGSFIDKRKVMELKGKGLSARAIAKSMECSITPILKILKEDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AZ3</accession>
    <name>Q97AZ3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transketolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0661085</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916925.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AZ3"/>
    <dbReference type="STRING" id="273116.gene:9381454"/>
    <dbReference type="PaxDb" id="273116-14324882"/>
    <dbReference type="GeneID" id="1441773"/>
    <dbReference type="KEGG" id="tvo:TVG0661085"/>
    <dbReference type="eggNOG" id="arCOG01051">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_1_2"/>
    <dbReference type="OrthoDB" id="6779at2157"/>
    <dbReference type="PhylomeDB" id="Q97AZ3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000129">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051157">
        <property type="entry name" value="PDH/Transketolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825:SF1">
        <property type="entry name" value="TRANSKETOLASE-LIKE PYRIMIDINE-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="4">
        <location>
            <begin position="4"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="316" mass="35158" checksum="0466EA75DB9C9A7C" modified="2001-10-01" version="1">MKTESLRDTYGKELVELGRKDPDIVVLDADLSSSTKTGYFAKEFPERFFNMGISEQSMVTTAAGLAISGKKPFVSTFAIFLMRTYEQIRQSICYNDVPVRFVVTHGGITVGEDGATHQIVEDVGIMSGLPNMSVIVPSDSVETKSVIDYLENIKHPHYVRLSREKFPVINDLSYEFKIGRGYVVKDGSDATVIANGIMVSKALEAANALKDKGIDLRIINMPSVKPIDKDIIIKAARETGRIITAEEHSIYNGLGSRVSEVVSENYPVIVKRIGMRDTFGKSGKAMELFSYFHMDVKDIIDYVIQSLEEKSYENIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-08-10" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AZ7</accession>
    <accession>C5J9S1</accession>
    <name>PSB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome subunit beta</fullName>
            <ecNumber evidence="1">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psmB</name>
        <name type="ordered locus">TV0662</name>
        <name type="ORF">TVG0656650</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="FN397805">
        <property type="protein sequence ID" value="CAZ48528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916921.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AZ7"/>
    <dbReference type="SMR" id="Q97AZ7"/>
    <dbReference type="STRING" id="273116.gene:9381450"/>
    <dbReference type="MEROPS" id="T01.002"/>
    <dbReference type="PaxDb" id="273116-14324878"/>
    <dbReference type="GeneID" id="1441769"/>
    <dbReference type="KEGG" id="tvo:TVG0656650"/>
    <dbReference type="eggNOG" id="arCOG00970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_7_2_2"/>
    <dbReference type="OrthoDB" id="6330at2157"/>
    <dbReference type="PhylomeDB" id="Q97AZ7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03764">
        <property type="entry name" value="proteasome_beta_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000049">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0888">Threonine protease</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_0000397480" description="Removed in mature form; by autocatalysis" evidence="1">
        <location>
            <begin position="1"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000397481" description="Proteasome subunit beta">
        <location>
            <begin position="9"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <sequence length="211" mass="23087" checksum="CA9487BE7B5776B4" modified="2001-10-01" version="1" precursor="true">MNQTLETGTTTVGITLTDAVIMATERRVTMENFIMHKNGKKLFQIDTYTGMTIAGLVGDAQVLVRYMKAELELYRLQRRVNMPIEAVATLLSNMLNQVKYMPYMVQLLVGGIDTSPHVFSIDAAGGSVEDIYASTGSGSPFVYGVLESQYNEKLSIDEGVDLVIRAISAAKQRDSASGGMIDVAVITKKDGYVQLPTEQIEARIKKLGLIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B38</accession>
    <name>KAD6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative adenylate kinase</fullName>
            <shortName evidence="1">AK</shortName>
            <ecNumber evidence="1">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV0621</name>
        <name type="ORF">TVG0613288</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Also has ATPase activity. Involved in the late maturation steps of the 30S ribosomal particles, specifically 16S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with uS11. Not a structural component of 40S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916880.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B38"/>
    <dbReference type="SMR" id="Q97B38"/>
    <dbReference type="STRING" id="273116.gene:9381409"/>
    <dbReference type="PaxDb" id="273116-14324837"/>
    <dbReference type="GeneID" id="1441728"/>
    <dbReference type="KEGG" id="tvo:TVG0613288"/>
    <dbReference type="eggNOG" id="arCOG01038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079096_0_1_2"/>
    <dbReference type="OrthoDB" id="8730at2157"/>
    <dbReference type="PhylomeDB" id="Q97B38"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00039"/>
        </source>
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</entry>
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        <name evidence="4" type="ORF">TVG0612669</name>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAB59762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="199" mass="22080" checksum="0A91F519EC20E26D" modified="2001-10-01" version="1">MLDSYRAKVDSILTPIAQALGFLSPNTISIISLISAGLTGIFFYLNYLYLSLLFLIISALFDAVDGKLARIQGKSSKRGDFIDHSFDRFSDVFILLGFTFSRYANVDLGIFALIGVILTSYMGTQAQALGLNRLYAGIMGRADRLVLMVIFIALQIPIGIIVRFPFLTLTPTNLMLLIFGILGSATVVQRFYIIFKELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B42</accession>
    <name>Q97B42_THEVO</name>
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        <submittedName>
            <fullName evidence="10">TVG0609673 protein</fullName>
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    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="tvo:TVG0609673"/>
    <dbReference type="eggNOG" id="arCOG01389">
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
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        <property type="entry name" value="Glyco_transf_21"/>
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            <end position="23"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAB59759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="352" mass="39746" checksum="425E1C3B26F67B15" modified="2001-10-01" version="1">MVFYVSIASLIAVGFSYIGIYKSNELKQRKNCRPKTLVIIPCKGLDLGLEDNLKAITKQNYDRADYLGVVDSLQDESVSVLEKVGINYIISDYTCEKCSGKVKAIATAIKKYTDYDAYVIADSDIRPNSNWLEDLVSPLCNDRVGLSTTFPYFVPIGGLASKVKAVWGTVGQSLMESKLTRFGWGGSLAFEKSLISDKMDYFTSNISDDSALTAICKSKKLDIAYVKEARPAIYTAENFPQFWEWANRQTALSINASPRVFTYGIIFYSLYIFEFFSAVLLTSISLAFVYLFIPVAIGVYRVYERSVIKSPIFAIIYLFIPFIYLANLLKAKRMGEIIWRGRQYKLQKILDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B45</accession>
    <name>Q97B45_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type II secretion system protein GspF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0604568</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916872.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B45"/>
    <dbReference type="STRING" id="273116.gene:9381402"/>
    <dbReference type="PaxDb" id="273116-14324829"/>
    <dbReference type="DNASU" id="1441720"/>
    <dbReference type="GeneID" id="1441720"/>
    <dbReference type="KEGG" id="tvo:TVG0604568"/>
    <dbReference type="eggNOG" id="arCOG01809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_479528_0_0_2"/>
    <dbReference type="OrthoDB" id="141855at2157"/>
    <dbReference type="PhylomeDB" id="Q97B45"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004704">
        <property type="entry name" value="PRK06041.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF2">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="528"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="81"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="552" mass="61787" checksum="5C5133517C185C9F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B56</accession>
    <name>Q97B56_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0593813 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0593813</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916860.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B56"/>
    <dbReference type="STRING" id="273116.gene:9381390"/>
    <dbReference type="PaxDb" id="273116-14324817"/>
    <dbReference type="GeneID" id="1441708"/>
    <dbReference type="KEGG" id="tvo:TVG0593813"/>
    <dbReference type="eggNOG" id="arCOG05362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137177_0_0_2"/>
    <dbReference type="OrthoDB" id="55635at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="131" mass="14975" checksum="E220EFB6339E8248" modified="2001-10-01" version="1">MKYAIREYKGKNVDLSKLSSIIENYFREENFVTQSSQHPRGYVIQAKKGGIFRTILGMDRAFTIVLVGDSSDFKVQIGVGKWLQDLGVAAIEAFLITPVVAFVEVPESLWSFELEHEFWQFLENQINLGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BC7</accession>
    <name>Q97BC7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucose-1-phosphate thymidylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0519949</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>dTTP + alpha-D-glucose 1-phosphate + H(+) = dTDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15225"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucose-1-phosphate thymidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.24" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916787.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BC7"/>
    <dbReference type="STRING" id="273116.gene:9381313"/>
    <dbReference type="PaxDb" id="273116-14324744"/>
    <dbReference type="GeneID" id="1441045"/>
    <dbReference type="KEGG" id="tvo:TVG0519949"/>
    <dbReference type="eggNOG" id="arCOG00664">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_1_2_2"/>
    <dbReference type="PhylomeDB" id="Q97BC7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008879">
        <property type="term" value="F:glucose-1-phosphate thymidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005907">
        <property type="entry name" value="G1P_thy_trans_s"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43532">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43532:SF1">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="246" mass="27451" checksum="450105CAF6A4577D" modified="2001-10-01" version="1">MELQGLITAAGLGTRSGASQFYRKEMFSIYDARDGKVVIRPIIDCVIYRMKISGIDDIIVVLDPKDEVTKKYIELSYPDVQIAYQTEKRGYGDAVHSALQFIHGPFLLNAGDGVLFDDNTEKRIVDSYKSGNRLIAFKVDDPRRYGVVEMDENGNIAGLEEKPNLPKSNLALAAIYILDKSVLNEIDTGKANVELTDAIDRTIKKGVKSDVIEIDRSEWISVGLADRYADVVSETRIKALRKIGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BC8</accession>
    <name>Q97BC8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfolipid biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0519952</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916786.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BC8"/>
    <dbReference type="STRING" id="273116.gene:9381312"/>
    <dbReference type="PaxDb" id="273116-14324743"/>
    <dbReference type="GeneID" id="1441044"/>
    <dbReference type="KEGG" id="tvo:TVG0519952"/>
    <dbReference type="eggNOG" id="arCOG04762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040971_1_0_2"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="PhylomeDB" id="Q97BC8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053578">
        <property type="entry name" value="UDP-sulfoquinovose_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041015">
        <property type="entry name" value="UDPsulfquin_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="390" mass="44892" checksum="2A254412FCBDC1FF" modified="2001-10-01" version="1">MKILIMGVDGYIGFPLALRLLTRGHDVYGIDNFITRKRVQKVGSDSALPISSFAFRNSVIKKKFGKGIGFFYGDATNPKFIYRVINEVRPDAIVHLAEQRSAPYSMIGLKEANETMVKNIVSTLNLIYAVKDITPEAHILKLGTMGEYGTPNIDIPEGFFEIEYHGRKDVLPFPRNASSWYHWTKVHDSNNLMFANKVWGTKVTDVMQGVVYGTRTEEIEKTGLYTRFDIDEVYGTALNRFTAEAIINEPITPYGKGGQTRGFLSLNDSIECLTLALENPPEFGEYRVFNQFDEKYSVNQLAEMVRDLYKEKFGKEPEIVHVDNPRVEKEEHYYNPEHEKLKALGYKRTKDIKSEIYDAIDDLNKFKERLMNLRNVIMPRTYWKKSAYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BE0</accession>
    <name>Q97BE0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator PadR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0504867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916774.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BE0"/>
    <dbReference type="STRING" id="273116.gene:9381299"/>
    <dbReference type="PaxDb" id="273116-14324731"/>
    <dbReference type="GeneID" id="1441032"/>
    <dbReference type="KEGG" id="tvo:TVG0504867"/>
    <dbReference type="eggNOG" id="arCOG00007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691634_0_0_2"/>
    <dbReference type="OrthoDB" id="57078at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="155" mass="17598" checksum="1E857C8BB7A86D0F" modified="2001-10-01" version="1">MDTYATRILILLSGREMTLYEISKRVDLYSTVSHGTVFPVIKKLLDGGFIKYRMEGNKKLYYATEKGQNYVTNIQNIGRELKEKIMEKSMGNALFYLNLLSSDEDAEALRTAIGLLMPTFIRMVEVVFRLYKDGKKDEIGSLVAKFGEMAQDGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BE7</accession>
    <name>Q97BE7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase expression formation protein HypE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0496806</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BE7"/>
    <dbReference type="STRING" id="273116.gene:9381292"/>
    <dbReference type="PaxDb" id="273116-14324724"/>
    <dbReference type="DNASU" id="1441025"/>
    <dbReference type="KEGG" id="tvo:TVG0496806"/>
    <dbReference type="eggNOG" id="arCOG00636">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041631_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BE7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06061">
        <property type="entry name" value="PurM-like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303:SF4">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="62"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="185"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="374" mass="41823" checksum="187D86511DBBD471" modified="2001-10-01" version="1">MLIITPRIKTLIMLVSIHPISTRMVMYLENPGKLDRKFFTESILRYTGYRRPEDKVPPKNGVDTGIISIGNGKVMAVTTDPFYFDPVFGYEDSAWFAYHILASDITTAGVYPEYMTVDLNLPISISNEEIARMWKTVHEEAYKYKTSIITGHTARYANTGFPMVGGATMFGFTDDDKYVTTAMARPGDIMIMTKTAGIEAASLLTRIFPNHVKKNLGEDKYRYGFELFRKLSTVDEAIQAIEFGTRKAITSMHDATEGGLLGAVYEVAEASGNGVSIDKEAIHVDDYVKEIHSLFGIDPLRSISEGTLLMTIDPEYADEFMKRLTKSGIDSYVIGKMTTKDEGIKFKDGSEPIGEPEKDPFWYAVEKSMKMGLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BG1</accession>
    <name>COBS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="1">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobS</name>
        <name type="ordered locus">TV0495</name>
        <name type="ORF">TVG0479924</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-ribazole + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-ribazole 5'-phosphate + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CobS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BG1"/>
    <dbReference type="STRING" id="273116.gene:9381277"/>
    <dbReference type="PaxDb" id="273116-14324710"/>
    <dbReference type="KEGG" id="tvo:TVG0479924"/>
    <dbReference type="eggNOG" id="arCOG04338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057426_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BG1"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00317">
        <property type="entry name" value="cobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148:SF1">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000146924" description="Adenosylcobinamide-GDP ribazoletransferase">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <sequence length="259" mass="28310" checksum="CF69B4F9D41EBE65" modified="2001-10-01" version="1">MMISGLRSSFSFFTLVPSRQKDIGNPITFLPLVVTVGALIGDSILYITWQFSHLIASFLSISSIIIYNGLNHFDATADLGDALMVRDKSRIPEVIKDHHVGAGGIFAVIFVYGIAVLSLARSTLYIGLVGILIGQVVSGSSMMISLIGSQPFVPGLADYFISLFRKHSVGYTIEFLAIPIIVSFIFSPLYVVIVALNLLIVQLTKTMISRRFGGINGDVIGFLGEFSRSLFIFMLIIIAHYNVASTYDIFSKMLSSFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BH9</accession>
    <name>Q97BH9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Arginine deiminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0464026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916735.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BH9"/>
    <dbReference type="STRING" id="273116.gene:9381258"/>
    <dbReference type="PaxDb" id="273116-14324691"/>
    <dbReference type="GeneID" id="1440993"/>
    <dbReference type="KEGG" id="tvo:TVG0464026"/>
    <dbReference type="eggNOG" id="arCOG03108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049514_0_0_2"/>
    <dbReference type="OrthoDB" id="371705at2157"/>
    <dbReference type="PhylomeDB" id="Q97BH9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016990">
        <property type="term" value="F:arginine deiminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019546">
        <property type="term" value="P:L-arginine deiminase pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003876">
        <property type="entry name" value="Arg_deiminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47271">
        <property type="entry name" value="ARGININE DEIMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47271:SF2">
        <property type="entry name" value="ARGININE DEIMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02274">
        <property type="entry name" value="ADI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01466">
        <property type="entry name" value="ARGDEIMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="418" mass="48095" checksum="89422AA66589718C" modified="2001-10-01" version="1">MRARSEWSPLREVMIHRPSIEIEYAMLAPRPFLFERVFSVKKAIEEHIRLEEILRENGIKVYRLEEEIIKKADYDANFRELLLNYVRSIVHFYGRIEDIRRAENEFAENIRQIDSKTLLDYLILEPSIDLKNDYENSGNYPTVYSNIPLANLYFMRDQQAVADSGVIIGNMKREQRKSESDITSFVLLNVFGETDAFKMPQNAHFEGGDFMPAGDFAFIGTGSRTDYNGAFQALSSGKIDCDEVLVVENPKYEFSQEDNMVNMHLDTYFNLAGDGIAVTSTYLAKRAKARVYGKKSRSRYEKLSETNLYEYLKEKGFNFIDLTVPEQLAYSSNFLTISDRKIISVNVESVIKRLISQRAFDKSTEEVIKTELSKIGEKIFPNRKEIKDFGIDFIDAELIELTGGYGGAHCMTATLNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BL9</accession>
    <name>Q97BL9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0425555</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760309.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BL9"/>
    <dbReference type="STRING" id="273116.gene:9381215"/>
    <dbReference type="PaxDb" id="273116-14324651"/>
    <dbReference type="GeneID" id="1440952"/>
    <dbReference type="KEGG" id="tvo:TVG0425555"/>
    <dbReference type="eggNOG" id="arCOG02682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="PhylomeDB" id="Q97BL9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF34">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="43"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="469" mass="51824" checksum="487DBA3A5BBF34D2" modified="2001-10-01" version="1">MYIRLVQYPSTGSGTGCTNIGGIWMGFFDDMDRMGIRPFHRKIEILASMGMFLDGYDLSVITMAILLMPTQLHFGKLDYILVNVSAFIGMIIGAPLLGRLSDRIGRKKIFGIDLLLFVVFAITAGLATNFAMLFISRLMLGIGIGGDYPISSTMVSEFSPVSKRGTLLYGMVGMYWLGSFFSAVMNYTFAFTPYFWRYTFIVGGVIAIPIIFLRMRIPESPRWLIGHGMTKSAESVVEDLTGRKPDVEEGKAEAEQEESVKFSSLFSSKFLASTIFVLSVWFLFDVASYGIGFYYPTLIDKLGLASHVLNTAGLRAIAESSMIVAVGAILGYLVAMPLADRIGRRILTISGLFVMFLLLLLGSVVKISTLLPVVAFFFSFVLFEQWVGAATLFYPTELFPTNYRSTIQGIATSVSRVGAIMGIVVFPLYPVFHSIFIFAILMALALIISVIFAPETKKTPLEKIWKSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BM1</accession>
    <name>Q97BM1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0422661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916692.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BM1"/>
    <dbReference type="STRING" id="273116.gene:9381213"/>
    <dbReference type="PaxDb" id="273116-14324649"/>
    <dbReference type="GeneID" id="1440950"/>
    <dbReference type="KEGG" id="tvo:TVG0422661"/>
    <dbReference type="eggNOG" id="arCOG03677">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891297_0_0_2"/>
    <dbReference type="OrthoDB" id="55603at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046057">
        <property type="entry name" value="DUF6015"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19479">
        <property type="entry name" value="DUF6015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="117" mass="13931" checksum="03DA555CDBE8E668" modified="2001-10-01" version="1">MGIVTREMLLQALNNIYGKKGMSRPDVEDLSDFVLSFFGYDDYVLDNVLSTAERDVFYNLEEYGILETYREEVSVMHGKIWRVNQWRYRKDKILELASEKDTVKKEVNIYDEIFKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BN0</accession>
    <name>Q97BN0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Intracellular proteinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0410258</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C56 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BN0"/>
    <dbReference type="STRING" id="273116.gene:9381203"/>
    <dbReference type="PaxDb" id="273116-14324640"/>
    <dbReference type="KEGG" id="tvo:TVG0410258"/>
    <dbReference type="eggNOG" id="arCOG00769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_44_4_2"/>
    <dbReference type="PhylomeDB" id="Q97BN0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd03169">
        <property type="entry name" value="GATase1_PfpI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006286">
        <property type="entry name" value="C56_PfpI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01382">
        <property type="entry name" value="PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42733">
        <property type="entry name" value="DJ-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42733:SF2">
        <property type="entry name" value="DJ-1_THIJ_PFPI FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51276">
        <property type="entry name" value="PEPTIDASE_C56_PFPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="2">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="187" mass="20797" checksum="048491D0DFE14F6C" modified="2001-10-01" version="1">MMTKVLIITGDAGESLEVMYPYQRLLEEGYEVDIAAPSAKYVQTVVHDFVPGFDTYTEKPGYLVKANKAFKDVNPEEYAAIVIPGGRAPEYIRNDPDVQRIVKYFFNKDAPVAELCHAPLILGSAGLLKGRKTAAYPALKSDVEIAGGTFIDEGAVVDRNIVSARAWPDHPSWMREFMKILKTKYPP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BP4</accession>
    <name>CBIA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="1">6.3.5.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobyrinic acid a,c-diamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiA</name>
        <name type="ordered locus">TV0411</name>
        <name type="ORF">TVG0399197</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cob(II)yrinate + 2 L-glutamine + 2 ATP + 2 H2O = cob(II)yrinate a,c diamide + 2 L-glutamate + 2 ADP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP and cobyrinate and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The a and c carboxylates of cobyrinate are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CobB/CbiA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.11" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916667.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BP4"/>
    <dbReference type="SMR" id="Q97BP4"/>
    <dbReference type="STRING" id="273116.gene:9381189"/>
    <dbReference type="PaxDb" id="273116-14324626"/>
    <dbReference type="GeneID" id="1440925"/>
    <dbReference type="KEGG" id="tvo:TVG0399197"/>
    <dbReference type="eggNOG" id="arCOG00106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022752_2_0_2"/>
    <dbReference type="OrthoDB" id="8896at2157"/>
    <dbReference type="PhylomeDB" id="Q97BP4"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05388">
        <property type="entry name" value="CobB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03130">
        <property type="entry name" value="GATase1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA/CobB_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002204">
        <property type="entry name" value="PRK01077.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873:SF1">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141283" description="Cobyrinate a,c-diamide synthase">
        <location>
            <begin position="1"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="GATase cobBQ-type" evidence="1">
        <location>
            <begin position="244"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="1">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <sequence length="454" mass="50355" checksum="8C2B1C0D821FF086" modified="2001-10-01" version="1">MKVPRVIIAGTNSGVGKTTTTLAVISALKNKGLKVKPYKVGPDYIDPQFHSLLAGATSDNLDLWMIPRERIFQLLADASTSFNISVIEGVMGLLDGFGSTDEGSTLDLARITGTPIILVIDGYGLSGSAAAIVKGFKDFSGDLLAGVIVTRVSGERHYDLIKKAIEDNTNVRVLGYIEKNDEVRLESRHLGLVQASELNSFSDYIERLSKVTHINVDGIIEIARASRDLDPKFSPLLSRVGYAKIAVAYDSAFDFYYEENFRVLRNLGAELVFFSPLNNEIPPEDTDGLYIGGGYPEVFAKKLAYAVDARENIAKLIKKGVPTLAECGGYMYLTRTIVGQDGIEYPGVGIVPAKTFLTDKLILGYREIVSKTSNMLLRHGETARGHEFHRSTIQFQDKVDHPFVLKYKDRFEEDGYYSNNVVASYVHIHFLSNIAIPKRFVEECIRYSKKREIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BQ5</accession>
    <name>Q97BQ5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">8-amino-7-ketopelargonate synthase</fullName>
            <ecNumber evidence="10">2.3.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG0388568</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>6-carboxyhexanoyl-[ACP] + L-alanine + H(+) = (8S)-8-amino-7-oxononanoate + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:42288"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9955"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78846"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="EC" id="2.3.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.47" evidence="10"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BQ5"/>
    <dbReference type="STRING" id="273116.gene:9381178"/>
    <dbReference type="PaxDb" id="273116-14324615"/>
    <dbReference type="KEGG" id="tvo:TVG0388568"/>
    <dbReference type="eggNOG" id="arCOG00113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BQ5"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008710">
        <property type="term" value="F:8-amino-7-oxononanoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000006">
        <property type="entry name" value="5-aminolevulinate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010962">
        <property type="entry name" value="AONS_Archaea/Firmicutes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004723">
        <property type="entry name" value="AONS_Archaea/Proteobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00858">
        <property type="entry name" value="bioF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01825">
        <property type="entry name" value="gly_Cac_T_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005394">
        <property type="entry name" value="PRK06939.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF3">
        <property type="entry name" value="LD36009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="6 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="43"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604723-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB59542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="396" mass="43143" checksum="0D521E544EC2F619" modified="2001-10-01" version="1">MINMQKYNWVDEEISALKAEGRYVPIRTIESAQGAWVKINGKQVLNMCSNNYLGFANHPETKKAAIEAIEEYGVGAGAVRSIAGTDEIHIKLEEKIASFKHSEAALVYQGGLLANLGTVPALVGKDDIIFSEELNHASIIDGTRLSPAKRIVYKHLSVDDLEKQAKENRSSGKKALVITDGVFSMDGDIAPLPEIVDIAEKYDIMTYVDDAHGEGVLGDHGRGIVNYFHLEDKVDIEMGTFSKALGSMGGFVAGSAEMIDLLKQKARPFLFSSALNPGDAAAVLKAIEILEKDDSLVKKLWENADLLKKSLADLGYNTGHSKTPITPVIIGDEKKTVELSTKLYEEKNVFASPIVYPTVPKGTARIRLMPSAVHTHEDIMTAVNAFGELGKKLHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BS3</accession>
    <name>Q97BS3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0368934 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0368934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916636.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BS3"/>
    <dbReference type="STRING" id="273116.gene:9381159"/>
    <dbReference type="PaxDb" id="273116-14324597"/>
    <dbReference type="GeneID" id="1440894"/>
    <dbReference type="KEGG" id="tvo:TVG0368934"/>
    <dbReference type="eggNOG" id="arCOG07405">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3003336_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="17"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="56" mass="6569" checksum="28F004362B262632" modified="2001-10-01" version="1">MTDNRRIMDIARMTKRGSSVRVTLPKKVLKKLNLKDEDLIAFYEGEDGRVYIELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BT6</accession>
    <name>Q97BT6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipid/polyisoprenoid-binding YceI-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0358547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916623.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BT6"/>
    <dbReference type="STRING" id="273116.gene:9381146"/>
    <dbReference type="PaxDb" id="273116-14324584"/>
    <dbReference type="GeneID" id="1440881"/>
    <dbReference type="KEGG" id="tvo:TVG0358547"/>
    <dbReference type="eggNOG" id="arCOG07357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115708_0_0_2"/>
    <dbReference type="OrthoDB" id="57018at2157"/>
    <dbReference type="PhylomeDB" id="Q97BT6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.110">
        <property type="entry name" value="Lipid/polyisoprenoid-binding, YceI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007372">
        <property type="entry name" value="Lipid/polyisoprenoid-bd_YceI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036761">
        <property type="entry name" value="TTHA0802/YceI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34406">
        <property type="entry name" value="PROTEIN YCEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34406:SF1">
        <property type="entry name" value="PROTEIN YCEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04264">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00867">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101874">
        <property type="entry name" value="YceI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipid/polyisoprenoid-binding YceI-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="172" mass="19558" checksum="F171A76EAE9A6C01" modified="2001-10-01" version="1">MERSLSKLTLNKAKSQISFTIDHLVISKVVGKFNTFGGYIETCGDLCVDYAELDIDSESLETGNSVRDRNLRGKNYFNCKKYPEILCTVRNVKLKNYFTNTVEVNVKIKDIEKTLKFDLFLLSYSLKNISVRVIGKINTGDFNLKWQSPFIPSAVIGSEARVEAYLTFEKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BT8</accession>
    <name>Q97BT8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-hydroxbutyryl-CoA dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0357199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BT8"/>
    <dbReference type="STRING" id="273116.gene:9381144"/>
    <dbReference type="PaxDb" id="273116-14324582"/>
    <dbReference type="KEGG" id="tvo:TVG0357199"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1212655_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BT8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="228" mass="25545" checksum="AE5FDE6C6F9F0245" modified="2001-10-01" version="1">MVIRMDNIRVRGYRDISFWQEDGIGLIVIKSDVNGIISRNCLDEMVSALGLASMDPNVKCVAFTGINKVFAKGMKPDGDDGRSLYELMQSSSSFLSMVYSLPKPIFSILNGDATNEGYEIALSSDLIISAEDANVGFNQEYQTILGGSFTAARFPLLAISKAQEAKNVDIVFKADSLLEDAKNYILKNFGNRMVQARRQKMYDISNKILLEKVWAYETYLMRSTERSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-12-06" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BV6</accession>
    <name>RS5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps5</name>
        <name type="ordered locus">TV0349</name>
        <name type="ORF">TVG0342708</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S4.</text>
    </comment>
    <comment type="domain">
        <text>The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916603.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BV6"/>
    <dbReference type="SMR" id="Q97BV6"/>
    <dbReference type="STRING" id="273116.gene:9381126"/>
    <dbReference type="PaxDb" id="273116-14324564"/>
    <dbReference type="GeneID" id="1440861"/>
    <dbReference type="KEGG" id="tvo:TVG0342708"/>
    <dbReference type="eggNOG" id="arCOG04087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_0_1_2"/>
    <dbReference type="OrthoDB" id="38155at2157"/>
    <dbReference type="PhylomeDB" id="Q97BV6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_A">
        <property type="entry name" value="Ribosomal_uS5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047866">
        <property type="entry name" value="Ribosomal_uS5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003125">
        <property type="entry name" value="PRK04044.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131664" description="Small ribosomal subunit protein uS5">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="S5 DRBM" evidence="1">
        <location>
            <begin position="46"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="221" mass="23864" checksum="009F46F13182EC92" modified="2001-10-01" version="1">MSDEWTPRTQLGKLVASGQIKTISEALKTKLPLKEYEIVDYLLPNIKDEVIDIKRAQRMTDSGRRMTYSITVVVGNEDGYIGLGIGRSKEAAPAIRKALINAKLNIMEIRRGCGSWECGCGRAHTLPFLVEGKSGSVRITLKPAPRGVGLAVGNVAKIILRMAGIEDAWGFAAGHTKTTVNYALAVYNALKETAKVRINPGIVLSTPIYSGSVINVSGHTD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BZ3</accession>
    <name>RL37A_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL43</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L37Ae</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein L43e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl37ae</name>
        <name type="ordered locus">TV0312</name>
        <name type="ORF">TVG0320982</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family. Putative zinc-binding subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916567.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BZ3"/>
    <dbReference type="SMR" id="Q97BZ3"/>
    <dbReference type="STRING" id="273116.gene:9381087"/>
    <dbReference type="PaxDb" id="273116-14324527"/>
    <dbReference type="GeneID" id="1440825"/>
    <dbReference type="KEGG" id="tvo:TVG0320982"/>
    <dbReference type="eggNOG" id="arCOG04208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141199_2_0_2"/>
    <dbReference type="OrthoDB" id="372011at2157"/>
    <dbReference type="PhylomeDB" id="Q97BZ3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000139857" description="Large ribosomal subunit protein eL43">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="80" mass="9058" checksum="C516FB7B4EB66BD7" modified="2001-10-01" version="1">MSKRTLKVGAAGRFGPRYGVTIRKEWNEIYKQKIALYTCPSCKKKRVKRLAAGIWVCRHCGYKFAGGAYNPAYEVKVVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C00</accession>
    <name>Q97C00_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0314249</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA damage repair.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nre family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916560.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C00"/>
    <dbReference type="STRING" id="273116.gene:9381080"/>
    <dbReference type="PaxDb" id="273116-14324520"/>
    <dbReference type="GeneID" id="1440818"/>
    <dbReference type="KEGG" id="tvo:TVG0314249"/>
    <dbReference type="eggNOG" id="arCOG04269">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039703_0_0_2"/>
    <dbReference type="OrthoDB" id="6609at2157"/>
    <dbReference type="PhylomeDB" id="Q97C00"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02096">
        <property type="entry name" value="Nre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033167">
        <property type="entry name" value="Nre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006979">
        <property type="entry name" value="Nre_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006978">
        <property type="entry name" value="Nre_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04895">
        <property type="entry name" value="Nre_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04894">
        <property type="entry name" value="Nre_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal Nre N-terminal" evidence="2">
        <location>
            <begin position="28"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Nre C-terminal" evidence="3">
        <location>
            <begin position="302"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="404" mass="46418" checksum="326784A8462C338A" modified="2001-10-01" version="1">MINGSDPRYNFNVAPYPYCRLCKGHKHMCGLRVCPILERFQEVFPRLDLHGDISTSTPPSIFIGEYNYPSVYGGIMSVVDPERPYARRDMFDLGMPEIIERNANLFRNVKTVSVHNLSEKTVEQIQEAAMALNELRVESRIVGKEKHFDFFDSPVGPMYKSENIKVYGNAKTPRAAQDVIYDIDLNSTEALKILYMNSIDINYITGLLSGGLLGIEKKRKLVPTRWSITAVDDTISRYLKSDALNYESIDKPMVFSYSRFGNHFNIILLPRGTAFEMQEYWEIGSLWGSDGRVSIDFEFESPRNSYASEITGAYYAARLAVFEYLSRIRRSASAIVLRRISPDYFAPLGVWVIRESVRNALKGKPAIFESLEDLFKVRLEIAEWKRYSPVIKRFMTQKTLTDNY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C09</accession>
    <name>GUAAB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>GMP synthase [glutamine-hydrolyzing] subunit B</fullName>
            <ecNumber>6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>GMP synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">guaAB</name>
        <name type="ordered locus">TV0296</name>
        <name type="ORF">TVG0306280</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916551.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C09"/>
    <dbReference type="SMR" id="Q97C09"/>
    <dbReference type="STRING" id="273116.gene:9381070"/>
    <dbReference type="PaxDb" id="273116-14324511"/>
    <dbReference type="GeneID" id="1440809"/>
    <dbReference type="KEGG" id="tvo:TVG0306280"/>
    <dbReference type="eggNOG" id="arCOG00085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_0_2"/>
    <dbReference type="OrthoDB" id="33844at2157"/>
    <dbReference type="PhylomeDB" id="Q97C09"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000002">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00345">
        <property type="entry name" value="GMP_synthase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026598">
        <property type="entry name" value="GMP_synthase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54810">
        <property type="entry name" value="GMP synthetase C-terminal dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140254" description="GMP synthase [glutamine-hydrolyzing] subunit B">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="GMPS ATP-PPase">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="27"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="310" mass="34643" checksum="A451A9E750C73C09" modified="2001-10-01" version="1">MSFSDYISRIKDNIRNSIKGKAIIAVSGGQDSSLLSLLASEVLGNNLLCVFIDTGLLRKNETGRVKDFFEKHSMNYVIVDAADTFINNLKGVTDPEEKRKIIGKTFIDVLSEQAQNFGAEYLLQGTIAPDWIESGGQKRDTIKSHHNVGGLPKDMKLKLVEPLRDYYKDEIRGMSKELGLPTDIQPFPGPGLAVRIIGEVTKEKLDLLRAVTDIVERKISEAMPSESRPWQYFAVLLPVRTTGVHGDRRAYGLTVGIRMIETTDAMTGTFSKPSWDLLEDISNTITDEIPEINRVVYDITNKPPATIEWE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-07-05" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C23</accession>
    <name>RNP4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 4</fullName>
            <shortName evidence="1">RNase P component 4</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Rpp21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp4</name>
        <name type="ordered locus">TV0282</name>
        <name type="ORF">TVG0293828</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59424" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59424.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C23"/>
    <dbReference type="SMR" id="Q97C23"/>
    <dbReference type="STRING" id="273116.gene:9381056"/>
    <dbReference type="PaxDb" id="273116-14324496"/>
    <dbReference type="KEGG" id="tvo:TVG0293828"/>
    <dbReference type="eggNOG" id="arCOG04345">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1493057_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97C23"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00757">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016432">
        <property type="entry name" value="RNP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004878">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000153866" description="Ribonuclease P protein component 4">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="98" mass="11963" checksum="D4F5F038CD090DA5" modified="2004-07-05" version="2">MSNNNYERLITKKDVYRVANERLYYLFSLAFQTGDERYIDLMERIGRRMDITLPQDIKRMYCKKCKKPYKNVRVRLKKNVITVTCLECGDIRRFQINR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C35</accession>
    <name>PYRC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroorotase</fullName>
            <shortName evidence="1">DHOase</shortName>
            <ecNumber evidence="1">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrC</name>
        <name type="ordered locus">TV0270</name>
        <name type="ORF">TVG0282239</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916527.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C35"/>
    <dbReference type="SMR" id="Q97C35"/>
    <dbReference type="STRING" id="273116.gene:9381043"/>
    <dbReference type="PaxDb" id="273116-14324484"/>
    <dbReference type="GeneID" id="1440785"/>
    <dbReference type="KEGG" id="tvo:TVG0282239"/>
    <dbReference type="eggNOG" id="arCOG00689">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_1_2"/>
    <dbReference type="OrthoDB" id="50279at2157"/>
    <dbReference type="PhylomeDB" id="Q97C35"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
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        <location>
            <position position="136"/>
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    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="1 2" key="1">
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
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        </source>
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                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    <evidence type="ECO:0000313" key="1">
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        </source>
    </evidence>
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</entry>
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    <accession>Q97C73</accession>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
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                <person name="Watanabe K."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
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            <dbReference type="PubMed" id="11121031"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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            <end position="301"/>
        </location>
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    <evidence type="ECO:0000259" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="335" mass="39462" checksum="12D408F40B3023A2" modified="2001-10-01" version="1">MNFYEEDFKVFSSYLDANKKKIRDSLLMDENSPLRRIYDRPFSDLYVEYSGSRSRPENLKISATDSSEFSRELYSGNFFILVRGYTRLGDSIYNKLEVSFLSIGPEDVKKETMIMMENVEHLSMLECLKKENPDFMLVDGSILSRILRCEKYQSSLDRENDYCLMLRELLEQRKKSNFAIAFVSKSSRSTLIRNSILDQIEDKALANLERNSNHFDHFLVKSLAKKPGYTKPLETSINIGNFSDHVLVTDILPRSEDLPIRVEMIGNRDRFEDLVDLMFWSYGGYKVYNIWLSEVDNLVKFRKREVETVYLKEFEKEIGLPFYETRGERRARTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C88</accession>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="GeneID" id="1440731"/>
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    <dbReference type="HOGENOM" id="CLU_094953_0_0_2"/>
    <dbReference type="OrthoDB" id="24039at2157"/>
    <dbReference type="PhylomeDB" id="Q97C88"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02109">
        <property type="entry name" value="arch_bact_SO_family_Moco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43032:SF4">
        <property type="entry name" value="OXIDOREDUCTASE MOLYBDOPTERIN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43032">
        <property type="entry name" value="PROTEIN-METHIONINE-SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase molybdopterin-binding" evidence="1">
        <location>
            <begin position="26"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="201" mass="23613" checksum="E4DAE4F9274AF5BD" modified="2001-10-01" version="1">MDRISVKNPGQRYIKNFIIYDALGEPDIDIRKWKLCVNGLVDKVKCYSFSDLQSMRQLEYISDFNCVTRWSIKDIVWKGPSLKEIIENSGPKREARWVMFWSADGYSTPVPIEYAMDEKAIIAISMNGAPLKKENGYPARPFLPQLYGWKSAKWITEIELIPEYKDGYWEAYGYAEVGRIEEEERFKGDEWKRIRRNSKMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C89</accession>
    <name>Q97C89_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0221806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916470.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C89"/>
    <dbReference type="STRING" id="273116.gene:9380984"/>
    <dbReference type="PaxDb" id="273116-14324428"/>
    <dbReference type="GeneID" id="1440730"/>
    <dbReference type="KEGG" id="tvo:TVG0221806"/>
    <dbReference type="eggNOG" id="arCOG02994">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116722_4_1_2"/>
    <dbReference type="OrthoDB" id="23670at2157"/>
    <dbReference type="PhylomeDB" id="Q97C89"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02222">
        <property type="entry name" value="cupin_TM1459-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37694">
        <property type="entry name" value="SLR8022 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37694:SF1">
        <property type="entry name" value="SLR8022 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="44"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="121" mass="13521" checksum="F3AE86EBA8EE245D" modified="2001-10-01" version="1">MNAEVSNGYVKSSEEVKGDVMDGGAVIKWLITHKNGAKNYSMRLITVQKGKSTPHHHHDYEHEIFIISGKVKVKLGEQEYMAGQDDFIFIPPNVEHGMNAEEDTRMICIVPIKAAKVLLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CE3</accession>
    <name>Q97CE3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5611 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0170125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916414.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CE3"/>
    <dbReference type="STRING" id="273116.gene:9380929"/>
    <dbReference type="PaxDb" id="273116-14324373"/>
    <dbReference type="GeneID" id="1441644"/>
    <dbReference type="KEGG" id="tvo:TVG0170125"/>
    <dbReference type="eggNOG" id="arCOG04412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152346_1_0_2"/>
    <dbReference type="OrthoDB" id="56086at2157"/>
    <dbReference type="PhylomeDB" id="Q97CE3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040713">
        <property type="entry name" value="DUF5611"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016800">
        <property type="entry name" value="UCP022080"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18446">
        <property type="entry name" value="DUF5611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022080">
        <property type="entry name" value="UCP022080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5611" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="96" mass="10843" checksum="3BC3DFCCFFC5842A" modified="2001-10-01" version="1">MRDYPVKKGIANTLESIADLLSGMGYQFSIEGDIIVTSLPGISRIEIKPEKRKLLVNTSDYSDGVDKLQIIKNYNDLIEKLTGYSAKERKKMMTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CF7</accession>
    <name>Q97CF7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0149603 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0149603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916399.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CF7"/>
    <dbReference type="PaxDb" id="273116-14324358"/>
    <dbReference type="GeneID" id="1441629"/>
    <dbReference type="KEGG" id="tvo:TVG0149603"/>
    <dbReference type="eggNOG" id="arCOG06951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158388_0_0_2"/>
    <dbReference type="OrthoDB" id="57340at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="131" mass="14626" checksum="746AA968033D5061" modified="2001-10-01" version="1">MRYQNIYKSILFYVVGLALLYLSIFLSNILKYNGHFISALPIVLPLVFSAASIGVAVILIMEKDSPWFFRTGIMSLVIGITLFLFGILTFYLGVESLVWAGSVVIGILFIIAAMVRLIIQGGLSTYRKIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CI2</accession>
    <name>Q97CI2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0126304 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0126304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CI2"/>
    <dbReference type="STRING" id="273116.gene:9380887"/>
    <dbReference type="PaxDb" id="273116-14324333"/>
    <dbReference type="KEGG" id="tvo:TVG0126304"/>
    <dbReference type="HOGENOM" id="CLU_2930410_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="60" mass="6844" checksum="B2254D52C86A2F7B" modified="2001-10-01" version="1">MFERLICKSKVGYELHLNLRLHNQLYLLHKDQLGNDTWKDELKGDAPLNGADGDKIHGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CK4</accession>
    <name>Q97CK4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cobalamin adenosyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0102626</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CK4"/>
    <dbReference type="STRING" id="273116.gene:9380865"/>
    <dbReference type="PaxDb" id="273116-14324311"/>
    <dbReference type="KEGG" id="tvo:TVG0102626"/>
    <dbReference type="eggNOG" id="arCOG00489">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083486_0_2_2"/>
    <dbReference type="PhylomeDB" id="Q97CK4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016030">
        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213:SF0">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE MMAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01923">
        <property type="entry name" value="Cob_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cobalamin adenosyltransferase-like" evidence="4">
        <location>
            <begin position="15"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="193" mass="21962" checksum="C8A5E503E1BBBCA5" modified="2001-10-01" version="1">MIKVKCTLFKVFGMFTRRGDTGETDLASRVRVGKDSPIVEVQGVIDELNAFIGQALVLTKWEDIRKDLFKIQNDLFVLGEDVSTGGKGRVVTREMIDYLEDRVNVLRKEIGKIELFVIPGGTLEATSVHIARTVSRRLERRIVFANHYTDINKNVLIYANRLSSLLFMIAMVSNKRQNVEEKIWPIHLPKDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CK9</accession>
    <name>Q97CK9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0096070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CK9"/>
    <dbReference type="STRING" id="273116.gene:9380860"/>
    <dbReference type="PaxDb" id="273116-14324306"/>
    <dbReference type="DNASU" id="1441579"/>
    <dbReference type="KEGG" id="tvo:TVG0096070"/>
    <dbReference type="eggNOG" id="arCOG00502">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073253_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CK9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052533">
        <property type="entry name" value="WalJ/YycJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47619:SF1">
        <property type="entry name" value="EXODEOXYRIBONUCLEASE WALJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47619">
        <property type="entry name" value="METALLO-HYDROLASE YYCJ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="260" mass="28791" checksum="4E794474FA9F06EE" modified="2001-10-01" version="1">MQTMTAYNNNAIYFKNIASGSKGNSTFIWDAHDLIVIDFGITMKKFKEAISELTLGGLNKSIFISHEHADHVSGIRSVKKVDGADVYTRPATAEAANIKAYEIDNEVVIGNFEILAFDVPHDAADPVAFTVKWKDLKISVVSDLGTVTENVISNIRDSDILAFESNHDVSMLKTGRYPASLKRRILSQHGHLSNEQSAEAISKAINESTEIVLTHLSQENNKPEIALNEVKSYLKNRDIKYKDVECADQYVGSSLKSLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CL9</accession>
    <name>Q97CL9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytochrome d oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG0081827</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916339.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CL9"/>
    <dbReference type="STRING" id="273116.gene:9380848"/>
    <dbReference type="PaxDb" id="273116-14324296"/>
    <dbReference type="GeneID" id="1441569"/>
    <dbReference type="KEGG" id="tvo:TVG0081827"/>
    <dbReference type="eggNOG" id="arCOG02721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030555_3_2_2"/>
    <dbReference type="OrthoDB" id="41618at2157"/>
    <dbReference type="PhylomeDB" id="Q97CL9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070069">
        <property type="term" value="C:cytochrome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016682">
        <property type="term" value="F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002585">
        <property type="entry name" value="Cyt-d_ubiquinol_oxidase_su_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30365:SF14">
        <property type="entry name" value="CYTOCHROME BD MENAQUINOL OXIDASE SUBUNIT I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30365">
        <property type="entry name" value="CYTOCHROME D UBIQUINOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01654">
        <property type="entry name" value="Cyt_bd_oxida_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006446">
        <property type="entry name" value="Cyt_quinol_oxidase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="323"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="361"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB59224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="455" mass="50529" checksum="5B49383837C7BB20" modified="2001-10-01" version="1">MAFITTFDRLLFAYSIGVHIILVTMSISTIVVLSVAEYLGIKYKDKYYDLLAKRLAKVFTIFFAIGTASGIVMAAELVILFPKFFSVVAAVAMVPFYLEIFAFMTETIFLIIYIYYWDFFKNRITHWILTFFIGAGTLGSAAFIVMVNAWMNTPNGFNIGVYEKTGAITDVNPWASLNSPSFMSEIAHVVPVTLLAGTMLIGGYFAYRYLKAREGEDRTFYKKGMKLTSALSMAYVILAGVSGSLAASDLIVNQPLKYSAIELNYLGGRAFEPEKLFGTIVNGHVVGAIKIPGLQSFLAGTGNPAYVIPKGLAYYPQSMWPPLIVHSLFDLMVGFGILIGLFLLAYLIMWILRSEPYSNRIMAGIYIILGFLAMFTMEDGWVVDEVGRQPWIIYNVMYVYQAINTSTSMIGPGIAMMAFYTLLIPVSFYFSARVFNRDSVSKEISASGSTTGVNF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CM6</accession>
    <name>TSR3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">16S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="2">2.5.1.157</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0075</name>
        <name type="ORF">TVG0075867</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
            <dbReference type="EC" id="2.5.1.157"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.157" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916332.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CM6"/>
    <dbReference type="SMR" id="Q97CM6"/>
    <dbReference type="STRING" id="273116.gene:9380841"/>
    <dbReference type="PaxDb" id="273116-14324289"/>
    <dbReference type="GeneID" id="1441562"/>
    <dbReference type="KEGG" id="tvo:TVG0075867"/>
    <dbReference type="eggNOG" id="arCOG04733">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035060_4_1_2"/>
    <dbReference type="OrthoDB" id="7441at2157"/>
    <dbReference type="PhylomeDB" id="Q97CM6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002621">
        <property type="entry name" value="PRK02287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000094425" description="16S rRNA aminocarboxypropyltransferase">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="E1QU22"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="164" mass="18707" checksum="FE44A50AA06305F8" modified="2001-10-01" version="1">MTGIYYLYLRQDDPKKATMRKLERFGLAKRVDIKGVGLKLVLTPYADIFLSREDAVLYEKYGLCVIEGSWNKIDSIKSLKFRIERRLPALLAANPVNYGKIGILSSVEATAAALYIIGYWDTAYALLSKFSWGLNFIKLNENPLNEYSTADRSEIKKIEESYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CN5</accession>
    <name>Q97CN5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0067031 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0067031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916323.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CN5"/>
    <dbReference type="STRING" id="273116.gene:9380832"/>
    <dbReference type="PaxDb" id="273116-14324280"/>
    <dbReference type="GeneID" id="1441553"/>
    <dbReference type="KEGG" id="tvo:TVG0067031"/>
    <dbReference type="eggNOG" id="arCOG07354">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1575038_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="169" mass="18480" checksum="22BB7C2A45AFC1A6" modified="2001-10-01" version="1">MDIKTVGRIGVVIVLVSLILFGSSIFYISRDTSSYSHTITPGSSIEITKEAAVSEYLSYKILFVSSSSSISSYLSSPDGTHYTFSHTMNTSGSMMAVQSGPWSLIVTNTGNTTENVEVVFSIVPQPVFFMLYISLALLAFGIAAIVLKPVRNAAIRWNKKREEKKALRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CN9</accession>
    <name>Q97CN9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0064561 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0064561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CN9"/>
    <dbReference type="STRING" id="273116.gene:9380828"/>
    <dbReference type="PaxDb" id="273116-14324276"/>
    <dbReference type="KEGG" id="tvo:TVG0064561"/>
    <dbReference type="eggNOG" id="arCOG02640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830696_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="140" mass="16352" checksum="4CEB31D6F3B0B807" modified="2001-10-01" version="1">MVEKSDDILDTSYFISREIKRMFLDYGRGMDRCSSIIQKCYSTFISMLEYNKGALKHLNAMLTAENDDKIREERIAIEKLEEKVDELKDDTFDYIYRNADDIPYLVFSHLVDLTHKVDDMLDDCEDAADLIITITRSITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CS5</accession>
    <name>Q97CS5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ArsA/GET3 Anion-transporting ATPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0025913</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916283.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CS5"/>
    <dbReference type="STRING" id="273116.gene:9380791"/>
    <dbReference type="PaxDb" id="273116-14324240"/>
    <dbReference type="GeneID" id="1441512"/>
    <dbReference type="KEGG" id="tvo:TVG0025913"/>
    <dbReference type="eggNOG" id="arCOG02849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_3_0_2"/>
    <dbReference type="OrthoDB" id="46198at2157"/>
    <dbReference type="PhylomeDB" id="Q97CS5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="258" mass="29603" checksum="D0BDEAC96A579DB4" modified="2001-10-01" version="1">MIEAFVGKGGVGKTTIAAAYSLICAEHGSTAIVSTDTMPSLHYIFPENTVNLDVFEISESEVSKLWKKRYGDQVYDIVSRFFDTGFEIIDHIANAPGVAEEFMISQIVGMGETKKFDYLVWDTPASSSTMHLLYLEKDFYEHLGRDIKFYFSMKDAFKTNKTLEILNEWRELSKKVWNEIENSKFYLVSTHDELSLLQSYEIEEDLKRMGISVSARIYNRTSQHEGYITIPELQGTAREIVEKAKPFLRKLFNSTHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CU8</accession>
    <name>Q97CU8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytochrome c3 hydrogenase [sulfhydrogenase] beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0004359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916260.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CU8"/>
    <dbReference type="STRING" id="273116.gene:9380768"/>
    <dbReference type="PaxDb" id="273116-14324217"/>
    <dbReference type="DNASU" id="1441488"/>
    <dbReference type="GeneID" id="1441488"/>
    <dbReference type="KEGG" id="tvo:TVG0004359"/>
    <dbReference type="eggNOG" id="arCOG05128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046702_0_0_2"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="PhylomeDB" id="Q97CU8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40447">
        <property type="entry name" value="ANAEROBIC SULFITE REDUCTASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40447:SF1">
        <property type="entry name" value="ANAEROBIC SULFITE REDUCTASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17179">
        <property type="entry name" value="Fer4_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="195"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="270"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="306" mass="35822" checksum="FC1755ED87CE7BD1" modified="2001-10-01" version="1">MIYKMTDKDMEILFERISKDFQSFGSKEENGDIVFGDIQRFSDISEEKSSYPPKDFLIERSENILSIPRAEKRAVFGVKSCDLRGFYIMDKQILGKDPFYTVKRENTMFINFVCEKMCSGGFCTSFGGPVLDEYDVQVLHKNGYFYVEASDKYEKYLFGFSESDDQNVFEEHKKEFESLYGRLNVDGIENRMKWSSNLWNKFANYCISCGACNYSCPTCYCVDVYDDDDGRKKEWDSCILEGFTRTAAGNVRPELSERLRQRFYHKFKYYKLSRGAYLCTGCNRCVDDCPVDIDIKEVITHDYDRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>P58275</accession>
    <name>LONH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Putative lon protease homolog</fullName>
            <ecNumber>3.4.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>ATP-dependent protease La homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV0156</name>
        <name type="ORF">TVG0165728</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks the conserved Ser-Lys catalytic dyad essential for proteolytic activity. Its enzyme activity is therefore unsure.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916411.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58275"/>
    <dbReference type="SMR" id="P58275"/>
    <dbReference type="STRING" id="273116.gene:9380926"/>
    <dbReference type="PaxDb" id="273116-14324370"/>
    <dbReference type="GeneID" id="1441641"/>
    <dbReference type="KEGG" id="tvo:TVG0165728"/>
    <dbReference type="eggNOG" id="arCOG02162">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_550564_0_0_2"/>
    <dbReference type="OrthoDB" id="64652at2157"/>
    <dbReference type="PhylomeDB" id="P58275"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00350">
        <property type="entry name" value="rubredoxin_like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42759">
        <property type="entry name" value="MOXR FAMILY PROTEIN"/>
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    </dbReference>
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    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
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        <location>
            <begin position="1"/>
            <end position="495"/>
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        <location>
            <begin position="471"/>
            <end position="495"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="472"/>
            <end position="486"/>
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    <feature type="binding site" evidence="1">
        <location>
            <begin position="52"/>
            <end position="59"/>
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        <ligand>
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    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="495" mass="55620" checksum="86F298A307DD930B" modified="2001-09-26" version="1">MGSVYVNFFEEYPDTSYIKIPNNPLDRVIGQDEAVRIASVAARQRRHLLLVGPPGVGKSMIAQALSFYIGRPNEEIRVVHNPQYPERPFVEIKTREEVMLEREEESSTSGTLIDPKDAPTSVAERLGYRCSKCGFYSSPTEMVCPNCNSPKAQIGTQGPFGDVFNVIGAAFGVQNNVDKVTLTRRNGDHDEIVVYERYNDKIRVLDEKTLERRRRLEKKSPSKTIVPIDRNPFVLATGASETELLGDVRHDPYGGHPQLGTLPYERVIAGAVHEAHEGVLFIDEITHLGNLQRYILTAMQEKVFPITGRNPQSAGASVRVDKVPADFILVAACNINDLPYILSPLRSRIVGNGYEILMKTTMKDTEENRMKYLQFIAQEINMDGKIPHMTMDAAQLIIEEGRKRAKLIDRKNNELTLRLRELGGLIRAAGDIAVFKGNKLIDKEDVEEAIKLYVPVEEKIMKEYGSMAAAYSSETTGSQRDSTYNYANMDDRSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q978A5</accession>
    <name>Q978A5_THEVO</name>
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    <reference evidence="2 3" key="1">
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60654.1"/>
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    </dbReference>
    <dbReference type="RefSeq" id="WP_010917742.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978A5"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="70" mass="7928" checksum="B539FDC195A5F8F1" modified="2001-10-01" version="1">MLNRYVYHIILVIIISIVTLIINVLSFLFKSVSNYLYLFDLAYVIGNSTHFVALSISSSGINIEDEKKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q978A6</accession>
    <name>Q978A6_THEVO</name>
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        <submittedName>
            <fullName evidence="1">TVG1564647 protein</fullName>
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    <organism evidence="1 2">
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978A6"/>
    <dbReference type="STRING" id="273116.gene:9382325"/>
    <dbReference type="PaxDb" id="273116-14325750"/>
    <dbReference type="GeneID" id="30928329"/>
    <dbReference type="KEGG" id="tvo:TVG1564647"/>
    <dbReference type="HOGENOM" id="CLU_1259168_0_0_2"/>
    <dbReference type="OrthoDB" id="372478at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="219" mass="26469" checksum="1586A7F273FC5CB1" modified="2001-10-01" version="1">MNDEIKIPKELPRNEIWANYFVEGFQSIFYYTLPDIWDARVIGILNIFNLLGITPIQAKLIDKLQEEFGKDRKFYWNHINGLAKKWKMLNVEYDIHKNKKVYFVNREFILHDDPLTKEKISDLFSMWVKVANLYYGTDESRRLKTVFFCGRCRFKNILKDMNKAEFLFFARNFMETSEYYCLNCGTYYGFFRKKLDASEYKDWKAFPFTYGKRRKDAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q978C4</accession>
    <name>Q978C4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1547121 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1547121</name>
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    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978C4"/>
    <dbReference type="STRING" id="273116.gene:9382307"/>
    <dbReference type="PaxDb" id="273116-14325732"/>
    <dbReference type="GeneID" id="24779985"/>
    <dbReference type="KEGG" id="tvo:TVG1547121"/>
    <dbReference type="HOGENOM" id="CLU_2519968_0_0_2"/>
    <dbReference type="OrthoDB" id="55932at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="82" mass="8834" checksum="B8D652DEEA7216D9" modified="2001-10-01" version="1">MVKFHPINRTMTVGTFTFFASLVVLAIGAAFHTDGTVLADSFLFGGFAFLISIVLLTVGAILGARSGKLQRRGDEIFRKNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q978C6</accession>
    <name>Q978C6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG1544340 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1544340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978C6"/>
    <dbReference type="STRING" id="273116.gene:9382305"/>
    <dbReference type="PaxDb" id="273116-14325730"/>
    <dbReference type="KEGG" id="tvo:TVG1544340"/>
    <dbReference type="eggNOG" id="arCOG04496">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204262_0_0_2"/>
    <dbReference type="OrthoDB" id="210639at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33258">
        <property type="entry name" value="TRANSPOSASE INSL FOR INSERTION SEQUENCE ELEMENT IS186A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33258:SF1">
        <property type="entry name" value="TRANSPOSASE INSL FOR INSERTION SEQUENCE ELEMENT IS186A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="107" mass="12701" checksum="B6075EDB460BC419" modified="2001-10-01" version="1">MYPYIMAHNYRIYRRFLTCIFTIYSQRWNIDIFFRTMKTYLKIDHLISRKINSIMVQIFTAMIAYIVLMIQDMLSCRMSIQEIISLAKHGISLPLRINEAGKASVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q978D2</accession>
    <name>Q978D2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1536123</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917715.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978D2"/>
    <dbReference type="STRING" id="273116.gene:9382299"/>
    <dbReference type="PaxDb" id="273116-14325724"/>
    <dbReference type="GeneID" id="1442175"/>
    <dbReference type="KEGG" id="tvo:TVG1536123"/>
    <dbReference type="eggNOG" id="arCOG02267">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_0_1_2"/>
    <dbReference type="OrthoDB" id="101311at2157"/>
    <dbReference type="PhylomeDB" id="Q978D2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="33201" checksum="62603AD6A24DE244" modified="2001-10-01" version="1">MLVLSYILLFASALFAWNMGSHYSGAVMGMAYGSKSLTIRQATVIAATFAFLGTITASSGVVHTYAFSLVGTTTIRQLTAALLGASVAILISTILKLPASTIQVYTFSLLFVGLASNIKLMYGGIEIVLSMWILMPLAAMLLSYLLAPKLVSKLADDSRKLKIIIIGSSVFSSFTLGSNDVSSAISPLVKVGILTGIVPYVFGGFFIGLGLITWGQRLIRRVGTEVVKTNAPLAAAAQLTEAIVIISGNLVGYNASINQTIIMSIYGAKRSMKSHVVTPMIFRSILLNWTVSSLISAVFGLFFLFILNLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q978E6</accession>
    <name>Q978E6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1521948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917702.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978E6"/>
    <dbReference type="SMR" id="Q978E6"/>
    <dbReference type="STRING" id="273116.gene:9382285"/>
    <dbReference type="PaxDb" id="273116-14325710"/>
    <dbReference type="DNASU" id="1442162"/>
    <dbReference type="GeneID" id="1442162"/>
    <dbReference type="KEGG" id="tvo:TVG1521948"/>
    <dbReference type="eggNOG" id="arCOG01884">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024745_0_0_2"/>
    <dbReference type="OrthoDB" id="8310at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050797">
        <property type="term" value="F:thymidylate synthase (FAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20175">
        <property type="entry name" value="ThyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003669">
        <property type="entry name" value="Thymidylate_synthase_ThyX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036098">
        <property type="entry name" value="Thymidylate_synthase_ThyX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934">
        <property type="entry name" value="FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934:SF1">
        <property type="entry name" value="FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02511">
        <property type="entry name" value="Thy1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69796">
        <property type="entry name" value="Thymidylate synthase-complementing protein Thy1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51331">
        <property type="entry name" value="THYX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="515" mass="59559" checksum="D18EAAE73B4FFAFC" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q978F1</accession>
    <name>Q978F1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PUA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1515782</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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                <person name="Makino K."/>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="71"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="153" mass="16908" checksum="B4E894C044D67DE1" modified="2001-10-01" version="1">MTQKHFLSKRELKVLVSSAAEIGIDLSGEEVEVSSSHKVSCYYIKGKPMAFQTDRLIPSVYLLNYKNPEKRAVVVDAGAEPHIINGSNVFAQGILSIDSSIKKGDIVFIKSEKGYFIGVGIAERNAEEIMNEKKGLAVRLIHHPDDDLMKAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="6">Amino acid permease/ SLC12A domain-containing protein</fullName>
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        <name evidence="7" type="ORF">TVG1498611</name>
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    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="273116.gene:9382262"/>
    <dbReference type="PaxDb" id="273116-14325689"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA_transporter"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="43"/>
        </location>
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        </location>
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        <location>
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        </location>
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        <location>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="474" mass="52351" checksum="4EE3F63188BD8998" modified="2001-10-01" version="1">MIEMRNNSHFKESKIGTLKSNVHGFVDLFALSTSSVAPAFSIAASYGVIAMYLGFHSISAIILVFPVWLASSFIFRKFNRLYPNAGASYHWGNIAVSKVYGSGQSWIITLAYLLSIPPIVIPAGEYTGDLLYSIGAIPQTALSNPVEIFSLGTFWVFVTLVASLLGARPTAKLTELFLAVEVSVIAMFVVIAVMSLHDSVVNRPSISWFIPPIASRNEYVNLLSSLPIIATVLDGWEIDSYASEESVNRERWPGTTGVIGLVLVFTIYVVTMTLMQSEVPIKVLSSSVDPLYAWGMFVAPKYAIVMDLAVIASTASSLWLTTYILSRAWYAMSREGLLPSYLGYLSKTRLVPYVGLILISALAELVNFLMIFSRSIEGFFSVLLSFSGVFLMAEFSMDSLTGIVLFLRNKDLEFRKLYLIVSVLTFVSFTAMICSWVYSELRYSAIFISLVMPALVIAMLERMKDRGKSSVQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q978G7</accession>
    <name>Q978G7_THEVO</name>
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        <submittedName>
            <fullName evidence="1">TVG1497634 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1497634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="PaxDb" id="273116-14325687"/>
    <dbReference type="GeneID" id="1442137"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DUF309"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="160" mass="18379" checksum="77A1FE9B923D0105" modified="2001-10-01" version="1">MRILIFTDRPLDLECGNRKVIIRKHTNMLEMDADLSSLDVLEGLEYKEARIIGDREPSFAFAPYSSKNVEILSREERYWEAHEVVEDMWRSLNHPSGLQKLILLLASQIHCQMGDCAHAEDLFIRYKDFLEQVGVEPVASTFTYPITILSSHVDLLSLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q978H3</accession>
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        <recommendedName>
            <fullName evidence="2">site-specific DNA-methyltransferase (cytosine-N(4)-specific)</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1491781</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978H3"/>
    <dbReference type="SMR" id="Q978H3"/>
    <dbReference type="STRING" id="273116.gene:9382253"/>
    <dbReference type="REBASE" id="685107">
        <property type="entry name" value="M.TvoIV"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325681"/>
    <dbReference type="KEGG" id="tvo:TVG1491781"/>
    <dbReference type="eggNOG" id="arCOG00890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027633_1_0_2"/>
    <dbReference type="OrthoDB" id="38200at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0680">Restriction system</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="382" mass="43468" checksum="233102B4F9CD1718" modified="2001-10-01" version="1">MIPQVARKLLYLYGSNAKTVFDPYCGTGTSLVEAMLLGINGIGTDLNPLAQLIAKAKTNAYIDYKSVISEIEKFNENADKLDPIVPNIKNIDFWFSKNVSIKLGKIKAYIESIEDENIKQFFMVAFSETVRESSNARKDEFKLVRYKEYKLKNWNPDPFEIINIKLNRNLKGLIDFKKKIASLNEIPYVKIYNYNSVNEIPNNDIPEDFADIVVTSPPYGDSHTTVAYGQYSRLSSEWLSLIGNENIDNKLMGGSKSPIIEDFPSLPLNEAIQAIEKKNKKRAIEVSSFYRDLQMSIKNVGRVLKSNGYACYVVANRTVNSVILPTSYAIKDIFEYYGFEYIKTYIRDIPNKRMPSVNSPTNVAGETSKTMLKEHIVVMKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q978N5</accession>
    <name>Q978N5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1426746</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978N5"/>
    <dbReference type="STRING" id="273116.gene:9382188"/>
    <dbReference type="PaxDb" id="273116-14325619"/>
    <dbReference type="KEGG" id="tvo:TVG1426746"/>
    <dbReference type="eggNOG" id="arCOG03651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943975_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="17"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="129" mass="14044" checksum="B038F229B550766B" modified="2001-10-01" version="1">MYESNLKPNQIGLAQAIFQGVSYVAPAADVAILLIITAGFAYGSTPLAVLFAWAIYFLFLNANYQFSKFTGSASGYYGYVSKSLGPTAGFITSLWYVMFQFFSLAAFGLLGFATFLYYVSPSLEKVPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q978P1</accession>
    <name>Q978P1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1421572 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1421572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917608.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978P1"/>
    <dbReference type="SMR" id="Q978P1"/>
    <dbReference type="STRING" id="273116.gene:9382182"/>
    <dbReference type="PaxDb" id="273116-14325613"/>
    <dbReference type="GeneID" id="1442066"/>
    <dbReference type="KEGG" id="tvo:TVG1421572"/>
    <dbReference type="eggNOG" id="arCOG00256">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1381489_0_0_2"/>
    <dbReference type="OrthoDB" id="358920at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050812">
        <property type="entry name" value="Preph/Arog_dehydrog"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005032">
        <property type="entry name" value="PRK06444.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="197" mass="22236" checksum="1F8D9BE0B657E92E" modified="2001-10-01" version="1">MKVGIIGKQGRLGRVLQAILDENGFDVVENNWDIAFLSVPIGAAISYIEKYDGPFIEVSSVKTPFKKYAGRIVSIHPLFGPASYKDGVHRTVLFIKDISMPEYRDLIAEMFRGYEIVDTTADVHDKEMAKSLALPYAVAIISKNIDTAFRTKSFDTFKGIVRILENENEEVMLDTINKNPYAQYMYDIVKNLGMVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q978P2</accession>
    <name>Q978P2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chorismate mutase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1420982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978P2"/>
    <dbReference type="STRING" id="273116.gene:9382181"/>
    <dbReference type="PaxDb" id="273116-14325612"/>
    <dbReference type="KEGG" id="tvo:TVG1420982"/>
    <dbReference type="eggNOG" id="arCOG07365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582970_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010950">
        <property type="entry name" value="Chorismate_mutase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01791">
        <property type="entry name" value="CM_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005031">
        <property type="entry name" value="PRK06443.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="178" mass="19717" checksum="1D9563A3E5CFFA3A" modified="2001-10-01" version="1">MVHFIEIEDLRQDLLENTKSILALLEKRREITRKIGKIKHNNGLPIRDAERELRVKSELCSNNPLLNVLFEASIFDQQSLNSAMDLRLSGDKDSLLFALGLILSKPGIIICVNRAVPRSLEAGCQLNGGHIVVGGNVTPDAVIDLKTGELAKLEGESLFISAAFIESRHRLNSVRLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q978P4</accession>
    <name>Q978P4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1419878 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1419878</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917605.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978P4"/>
    <dbReference type="STRING" id="273116.gene:9382179"/>
    <dbReference type="PaxDb" id="273116-14325610"/>
    <dbReference type="GeneID" id="1442063"/>
    <dbReference type="KEGG" id="tvo:TVG1419878"/>
    <dbReference type="eggNOG" id="arCOG00394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2730561_0_0_2"/>
    <dbReference type="OrthoDB" id="350804at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13730">
        <property type="entry name" value="HTH_36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="76" mass="8700" checksum="75307139D849F5A6" modified="2001-10-01" version="1">MYGTFDLKSLPPSARLVLAYLRSAKISDVARIMENTGLSRRSVMNSVKMLKELGLIDIQVCLSDTRRRYYCVKIFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q978R8</accession>
    <name>Q978R8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1393808</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978R8"/>
    <dbReference type="STRING" id="273116.gene:9382155"/>
    <dbReference type="PaxDb" id="273116-14325586"/>
    <dbReference type="KEGG" id="tvo:TVG1393808"/>
    <dbReference type="eggNOG" id="arCOG01345">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152992_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978R8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="109" mass="12774" checksum="54841507D0240243" modified="2001-10-01" version="1">MYMKEYSIFREIINDMDTLKRHISVINVLLREQPIGIIKLSQETGIPEHKIRYSLRILEQENIISPSREGAILTPDFIEHKEQIIADAEKVLEEATKIAEDVRKMLEKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q978T4</accession>
    <name>Y1331_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Putative esterase TV1331</fullName>
            <ecNumber>3.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV1331</name>
        <name type="ORF">TVG1373162</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.-"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917566.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978T4"/>
    <dbReference type="SMR" id="Q978T4"/>
    <dbReference type="STRING" id="273116.gene:9382139"/>
    <dbReference type="PaxDb" id="273116-14325570"/>
    <dbReference type="GeneID" id="1441448"/>
    <dbReference type="KEGG" id="tvo:TVG1373162"/>
    <dbReference type="eggNOG" id="arCOG00777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_3_3_2"/>
    <dbReference type="OrthoDB" id="24516at2157"/>
    <dbReference type="PhylomeDB" id="Q978T4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156696" description="Putative esterase TV1331">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="133" mass="14736" checksum="A4AB08EF94588A3E" modified="2001-10-01" version="1">MFDGSDLKNLFSLDGFLKTIQFEPEYIREGEISIIVPLRNNLLRIGEIMNGGAVMAISDAIGGLSAMTYPGIVNQVTVSFNTEFMRPIAKGPVRFISRVDRIGKSIAYVEVLVYDGENLLSSKSTGVYFLYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q978V0</accession>
    <name>Q978V0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7 8">Uroporphyrinogen decarboxylase</fullName>
            <ecNumber evidence="3 7 8">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG1357015</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>uroporphyrinogen III + 4 H(+) = coproporphyrinogen III + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="3 7 8"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917550.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978V0"/>
    <dbReference type="STRING" id="273116.gene:9382123"/>
    <dbReference type="PaxDb" id="273116-14325554"/>
    <dbReference type="GeneID" id="1441432"/>
    <dbReference type="KEGG" id="tvo:TVG1357015"/>
    <dbReference type="eggNOG" id="arCOG03323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040933_0_1_2"/>
    <dbReference type="OrthoDB" id="124836at2157"/>
    <dbReference type="PhylomeDB" id="Q978V0"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091">
        <property type="entry name" value="METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091:SF169">
        <property type="entry name" value="UROPORPHYRINOGEN DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="10">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="11">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB60457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="332" mass="37430" checksum="FFB1EA39B161020C" modified="2001-10-01" version="1">MNAFLKALHGLDHDYIPVWFMRQAGRYLNVYKDYRKKLGLEGMMTDADVIVKITHSPVDMIGVDAAIIFADITTPLPGLGFKVRFEDNVGPIVLNNLSDNGFSGINEFDEASFNHPVLKAISMYREMYREPLIGFCGSPVTLLSYLIAGSFDKDLAKTKRLMLTDPSKYKEISTLLTDASVKYAKLQIKKGVDAFQLFDSWAGYLSPRQYKEFAVPYINDILSEIAGKVPTIYFSTMTSSFVFQSGIISDFISVDWRVEMDKVVKESGDYGLQGNLDPFIVNYDYAFAESEHIINAVKGNNRYIFNTGHGILPDTDPKRLIEIVKYVHSVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q978W4</accession>
    <name>Q978W4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Electron transfer flavoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1342946</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917536.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978W4"/>
    <dbReference type="STRING" id="273116.gene:9382108"/>
    <dbReference type="PaxDb" id="273116-14325540"/>
    <dbReference type="DNASU" id="1441418"/>
    <dbReference type="GeneID" id="1441418"/>
    <dbReference type="KEGG" id="tvo:TVG1342946"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050977_0_0_2"/>
    <dbReference type="OrthoDB" id="7950at2157"/>
    <dbReference type="PhylomeDB" id="Q978W4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039651">
        <property type="entry name" value="FixC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43624">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-QUINONE OXIDOREDUCTASE YDIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43624:SF2">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-QUINONE OXIDOREDUCTASE YDIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="5">
        <location>
            <begin position="4"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="428" mass="46573" checksum="CCE8E68C534FE32E" modified="2001-10-01" version="1">MSEFDVAVIGAGPAGSSAAIRLAQNGMNVLLVERGDPPGTKNVSGAVLWGNELAKVVPDWERSAPVERYVTRKEVAFLTEKSKIAISFSSEELQNSKSGKIIVRGKFDQWLAKKAKDAGAMLVTGVTVDRLAIDNGKVIGIQQDNDVITADAVVVAEGANPRVLINSGLRPKLSDRDVALGIKETIKLPENVINERFNVDSKSGFASEFVLGFLDDGLLSGGFLYTNKDTVSLGIVASLDRLRANGVTHSYDIMGKFEEHPFIKNLIKDGIPDEYAAHLVPEGGISSMPKLYGNGYLVAGDAAAFTFSNGMVLQGINYAITSGILAADTIIENRNNINENTLAKYVDKLRESYVLSDLYNFNGIQNVTWSDFVQKSLPTLAESVMMSLFDEKGMPKKHLRQIVTDAMKSGHMKYSDLITNGYRVIKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q978W9</accession>
    <name>Q978W9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Endonuclease III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1336820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978W9"/>
    <dbReference type="STRING" id="273116.gene:9382103"/>
    <dbReference type="PaxDb" id="273116-14325535"/>
    <dbReference type="KEGG" id="tvo:TVG1336820"/>
    <dbReference type="eggNOG" id="arCOG00461">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_6_0_2"/>
    <dbReference type="PhylomeDB" id="Q978W9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359">
        <property type="entry name" value="A/G-SPECIFIC ADENINE GLYCOSYLASE/ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359:SF19">
        <property type="entry name" value="DNA REPAIR GLYCOSYLASE MJ1434-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001435">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="5">
        <location>
            <begin position="52"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="244" mass="27947" checksum="B2F3B70627EF43F5" modified="2001-10-01" version="1">METYRVRCNRILGMTSNPPNFANIYEELFRYYGDLGWWPAETKDEIVIGAILTQNTSWKNVEKAIANLKSHGITKLEDVCKIEKNEIAKLIRSSGFYNQKAERLKAVSCLIVGEFNGIDRIKDIDAFAERLKSIKGIGQETLNSILLYALDAPVFVIDKYTVRFLERYSSFDEVDSIKKSVEEQLADVKLMQNFHAMIVQLSKDFCRKEPICMKCPLKACSTGQRVRKELYSNNSMDPYGSNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q979B0</accession>
    <name>Q979B0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dihydrodipicolinate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1289977</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917486.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979B0"/>
    <dbReference type="STRING" id="273116.gene:9382057"/>
    <dbReference type="PaxDb" id="273116-14325490"/>
    <dbReference type="GeneID" id="1441368"/>
    <dbReference type="KEGG" id="tvo:TVG1289977"/>
    <dbReference type="eggNOG" id="arCOG04172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_5_1_2"/>
    <dbReference type="OrthoDB" id="33636at2157"/>
    <dbReference type="PhylomeDB" id="Q979B0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00408">
        <property type="entry name" value="DHDPS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0704">Schiff base</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="3">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="289" mass="32352" checksum="343B9A2846FB58E9" modified="2001-10-01" version="1">MYNGIVTPMLTPFNRAGEIDYDATRLLIQDLKGYGVSGLFPMGSTGLFPMFSLEERKKFLQFVSENRNNLTVYAGVGSSSTQESIELSKFTEDVGIEVRVLMPTYYIKPDEEWMYKHFSEVISASSNDLFIYNIPQLAGSWVPENVIKKLKSEFSQVKGIKDSSGDMRFFSRIVTLVSKDFSVFQGQDDLLLTSMALGANGGVCGLSNISGSIVDLYKAFQSGDLKKSREIQLNKVNPLMYAVNAPTFPSGYYYAFYKKHNINGGYRSPMVQPSKEHSRVIDEATSQQI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-08" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q979B7</accession>
    <name>SYM_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="1">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metG</name>
        <name type="ordered locus">TV1244</name>
        <name type="ORF">TVG1284612</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of L-methionine to tRNA(Met). Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:13482"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917478.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979B7"/>
    <dbReference type="SMR" id="Q979B7"/>
    <dbReference type="STRING" id="273116.gene:9382049"/>
    <dbReference type="PaxDb" id="273116-14325482"/>
    <dbReference type="GeneID" id="1441360"/>
    <dbReference type="KEGG" id="tvo:TVG1284612"/>
    <dbReference type="eggNOG" id="arCOG00810">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_1_2_2"/>
    <dbReference type="OrthoDB" id="371856at2157"/>
    <dbReference type="PhylomeDB" id="Q979B7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001100">
        <property type="entry name" value="PRK00133.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000139203" description="Methionine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="1">
        <location>
            <begin position="334"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <sequence length="543" mass="62931" checksum="60E820FB6E461BA7" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-04-05" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q979C2</accession>
    <name>RIBL_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD synthase</fullName>
            <ecNumber evidence="1">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FMN adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Flavin adenine dinucleotide synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ribL</name>
        <name type="ordered locus">TV1239</name>
        <name type="ORF">TVG1279046</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="2009-05" db="PDB data bank">
            <title>The crystal structure of the lipopolysaccharide core biosynthesis protein from Thermoplasma volcanium GSS1.</title>
            <authorList>
                <consortium name="Midwest center for structural genomics (MCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) IN COMPLEX WITH AMP</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the AMP portion of ATP to flavin mononucleotide (FMN) to produce flavin adenine dinucleotide (FAD) coenzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal FAD synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917473.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="3GLV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.99 A"/>
        <property type="chains" value="A/B=1-142"/>
    </dbReference>
    <dbReference type="PDBsum" id="3GLV"/>
    <dbReference type="AlphaFoldDB" id="Q979C2"/>
    <dbReference type="SMR" id="Q979C2"/>
    <dbReference type="STRING" id="273116.gene:9382044"/>
    <dbReference type="PaxDb" id="273116-14325477"/>
    <dbReference type="DNASU" id="1441355"/>
    <dbReference type="GeneID" id="1441355"/>
    <dbReference type="KEGG" id="tvo:TVG1279046"/>
    <dbReference type="eggNOG" id="arCOG01222">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034585_2_1_2"/>
    <dbReference type="OrthoDB" id="1912at2157"/>
    <dbReference type="PhylomeDB" id="Q979C2"/>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q979C2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046444">
        <property type="term" value="P:FMN metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02170">
        <property type="entry name" value="cytidylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02115">
        <property type="entry name" value="FAD_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050385">
        <property type="entry name" value="Archaeal_FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024902">
        <property type="entry name" value="FAD_synth_RibL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793:SF1">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000406287" description="FAD synthase">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="14"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="3"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="38"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="52"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="66"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="76"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="113"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2">
        <source ref="2"/>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="3GLV"/>
        </source>
    </evidence>
    <sequence length="142" mass="16009" checksum="B9A106F982D0022E" modified="2001-10-01" version="1">MIRVMATGVFDILHLGHIHYLKESKKLGDELVVVVARDSTARNNGKIPIFDENSRLALISELKVVDRAILGHEGDMMKTVIEVKPDIITLGYDQKFDEAELQSKINKLGITVKIVRISKYDGQLNSSSSVRKKIMELIGERY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q979C3</accession>
    <name>MPTA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP cyclohydrolase MptA</fullName>
            <ecNumber evidence="1">3.5.4.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP cyclohydrolase IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mptA</name>
        <name type="ordered locus">TV1238</name>
        <name type="ORF">TVG1279013</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts GTP to 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate, the first intermediate in the biosynthesis of coenzyme methanopterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 2',3'-cyclic phosphate + formate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25860"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58854"/>
            <dbReference type="EC" id="3.5.4.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase IV family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.39" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917472.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979C3"/>
    <dbReference type="SMR" id="Q979C3"/>
    <dbReference type="STRING" id="273116.gene:9382043"/>
    <dbReference type="PaxDb" id="273116-14325476"/>
    <dbReference type="GeneID" id="1441354"/>
    <dbReference type="KEGG" id="tvo:TVG1279013"/>
    <dbReference type="eggNOG" id="arCOG04301">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062816_1_0_2"/>
    <dbReference type="OrthoDB" id="53087at2157"/>
    <dbReference type="PhylomeDB" id="Q979C3"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044682">
        <property type="term" value="F:GTP cyclohydrolase IV activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.270.10">
        <property type="entry name" value="Urate Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01527_A">
        <property type="entry name" value="GTP_cyclohydrol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003801">
        <property type="entry name" value="GTP_cyclohydrolase_FolE2/MptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022840">
        <property type="entry name" value="GTP_cyclohydrolase_MptA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00294">
        <property type="entry name" value="GTP cyclohydrolase MptA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445">
        <property type="entry name" value="GTP CYCLOHYDROLASE MPTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445:SF1">
        <property type="entry name" value="GTP CYCLOHYDROLASE MPTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02649">
        <property type="entry name" value="GCHY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147754" description="GTP cyclohydrolase MptA">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="site" description="May be catalytically important" evidence="1">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01527"/>
        </source>
    </evidence>
    <sequence length="284" mass="31808" checksum="ED1F698243BD96BC" modified="2001-10-01" version="1">MIDFLDVQASTPDIRISVDKVGIRRMKFPIKIGDEVAILSADLYIDIPQTRKGADMSRAVESIQSVLSRPSINLESLGIEICKEALGRFNYASRVEVKINGEYYKKSNGGYDEISLYIRTKCGIDGNIENLTGLSYEAITACPCAMETTRALISKDIPDSENVLYYIPTVTHNQRNRTKLIVSNNAGKISFWDIYKVLESVQGKPLESLLKRIDEGKLVYEAHKKPKFVEDVVREVAFAAVTLLPLSDDDMVIVSSDSEESIHPHNAYASMKKRALDLKKELNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q979E1</accession>
    <name>Q979E1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-responsive regulatory protein [Lrp]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1254131</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979E1"/>
    <dbReference type="STRING" id="273116.gene:9382025"/>
    <dbReference type="PaxDb" id="273116-14325458"/>
    <dbReference type="KEGG" id="tvo:TVG1254131"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="PhylomeDB" id="Q979E1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="129" mass="14896" checksum="9A39D0ED1C74B3B6" modified="2001-10-01" version="1">MLETDCSLTYEEIAKMTGKSLWTIRDRILALKKRGVIKSCRAEIDYDKLGLPCRAIIGFNVPPDKIDDFVEQAKKDKRIKKFIITTGSRRFHIKIIGKQCGEIREYARTILPKFGVFDVDFEVVLDEII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-10" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q979E4</accession>
    <name>NEP1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase Nep1</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA (pseudouridine-N1-)-methyltransferase Nep1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">nep1</name>
        <name type="ordered locus">TV1217</name>
        <name type="ORF">TVG1248551</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a pseudouridine in rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46696"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:13933"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917449.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979E4"/>
    <dbReference type="SMR" id="Q979E4"/>
    <dbReference type="STRING" id="273116.gene:9382021"/>
    <dbReference type="PaxDb" id="273116-14325455"/>
    <dbReference type="GeneID" id="1441331"/>
    <dbReference type="KEGG" id="tvo:TVG1248551"/>
    <dbReference type="eggNOG" id="arCOG04122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055846_1_3_2"/>
    <dbReference type="OrthoDB" id="7612at2157"/>
    <dbReference type="PhylomeDB" id="Q979E4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00554">
        <property type="entry name" value="NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023503">
        <property type="entry name" value="Ribosome_NEP1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003204">
        <property type="entry name" value="PRK04171.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000158625" description="Ribosomal RNA small subunit methyltransferase Nep1">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="197"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes Arg-62" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="1">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="1">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="222" mass="25904" checksum="19207A69EEB0D2D3" modified="2001-10-01" version="1">MLHLIVADSELETIPEEMLDDPGVRRFAKKRNKRVDRMILDSNYMHAFIDKYYPGESKRRGRPDIIYILLEMAQESILNRKNLLRTYIHTRNDFVIKISPITRMPKSYNRFIGLFEDLFEKEIITNNGKTLLSLERMKLDELLESLKKDRTILLHPKGEFKKPSEFISTEDIAAIIGGFSEGDFRSDVSNIPEKYSIFRDELTIWSVGLEIVASYERAIGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q979F6</accession>
    <name>Q979F6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable transcription termination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nusA</name>
        <name evidence="9" type="ORF">TVG1233261</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates in transcription termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917437.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979F6"/>
    <dbReference type="STRING" id="273116.gene:9382007"/>
    <dbReference type="PaxDb" id="273116-14325443"/>
    <dbReference type="GeneID" id="1441319"/>
    <dbReference type="KEGG" id="tvo:TVG1233261"/>
    <dbReference type="eggNOG" id="arCOG01760">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131906_1_0_2"/>
    <dbReference type="OrthoDB" id="4116at2157"/>
    <dbReference type="PhylomeDB" id="Q979F6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_A">
        <property type="entry name" value="NusA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058582">
        <property type="entry name" value="KH_NusA_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010212">
        <property type="entry name" value="NusA_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006258">
        <property type="entry name" value="PRK08406.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26594">
        <property type="entry name" value="KH_NusA_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="NusA-like second KH" evidence="8">
        <location>
            <begin position="76"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="142" mass="16503" checksum="49798BF3FA8EF066" modified="2001-10-01" version="1">MKEITVDNEIMAHIAIFEKIAKVELMECVENEDMALFIVGEHKMAEMFKKNKDVISDLKDKINKHILVAEISRDLLTFVRNIFFRYGVNEIQISWKNNRTDIVVGVKQEEIGKVIGKEGKNIKLFRDAVSRYFNINTISVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q979H3</accession>
    <name>Q979H3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Multidrug-efflux transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1217101</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917421.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979H3"/>
    <dbReference type="STRING" id="273116.gene:9381989"/>
    <dbReference type="PaxDb" id="273116-14325426"/>
    <dbReference type="GeneID" id="1441303"/>
    <dbReference type="KEGG" id="tvo:TVG1217101"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="PhylomeDB" id="Q979H3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17321">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="526"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="8"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="587" mass="62931" checksum="067078EDF6E399E5" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q979H6</accession>
    <name>Q979H6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Shikimate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1213986</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="273116.gene:9381986"/>
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    <dbReference type="KEGG" id="tvo:TVG1213986"/>
    <dbReference type="eggNOG" id="arCOG02691">
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Sugar_transporter_CS"/>
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        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
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    </dbReference>
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            <end position="60"/>
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            <end position="448"/>
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            <begin position="30"/>
            <end position="450"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="461" mass="50274" checksum="F4EA80954FD218F4" modified="2001-10-01" version="1">MLRVDVAMADTTNIEGFKALERFGRDSAKATGSQFLGFLLDSFDLTMILSIAPVVAAVLLPPSSPLIAVFSVILAYSLTIIFRPVGSAIFGHIGDRLGRRTDLIITVLGLGIVSALTSALPTYASVGILSFVLFIIIRIFVGIFAGGEYSAGHPFVMEWTPFRWRGLISGLVQGGFSFGALAAAATVAVFTHIYGETGLVSYAWRYLFLTTLIPAAIALAIRLSMKDTPIFTELKEKNKIRRSPFIDLFKKPVRKDFLQVMIWMTGMFFYAYALFAYVPTILEHAPSAWSSNLGLATTVYTIGTAGAFFGAFGFGIASQKVGRRKLTLIWAIMTIIISVPMYYGLMRAAELGNVPLAVLMSIIIGIVTQGPWGIVPVYLSERFKTAVRASGVGFGYSSGIFIGGWFSIYVPLMHNYLFKSIDTSTNIWFSTAVLLMIGAIIIFIGQWIGPETLGTRLRSVE</sequence>
</entry>
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            <fullName evidence="7">Probable membrane transporter protein</fullName>
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            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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            <topology evidence="1 7">Multi-pass membrane protein</topology>
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        <property type="entry name" value="TSUP"/>
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        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TauE"/>
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    </dbReference>
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    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="327" mass="35276" checksum="C51CFAD928BD36FB" modified="2001-10-01" version="1">MLTIIEIVIRFLSILIGAFIAGLIGSLTGLGGGTVLVPVLTLFYGVPIIYATGASLISTIATSAGSASAYAKEKIANIRLGVGLEVATTTGAIVGSLTAVYIYDHHLSWLVYVIFGMVLLGSLIPTIQRGKYEIPKILKPDWTTRIFQLSGSYYDAKLHKNVEYSGVRWWLGEIIMFFAGLISGLLGIGSGALKVLGMDWAINLPMKVTTTSSNFMIGITAATGSSIYWYAGYIQPFIAAATAIGVLIGAFLGAKILVRITNRSIRWIFFAILMFLGIQMVLKGLYIINVITISPYIQFAISTIISIISIIILFQVMKKIEARGDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q979K6</accession>
    <name>Q979K6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG1184459 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1184459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979K6"/>
    <dbReference type="STRING" id="273116.gene:9381955"/>
    <dbReference type="PaxDb" id="273116-14325393"/>
    <dbReference type="DNASU" id="1441271"/>
    <dbReference type="KEGG" id="tvo:TVG1184459"/>
    <dbReference type="eggNOG" id="arCOG00683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_13_1_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="105" mass="11870" checksum="C137FE501E1274F7" modified="2001-10-01" version="1">MLIYQIKYKAEEIGITVIMQNEDYTSKCSFLDNESIEHHDTYMGKRIHRGIFRSATGKLIHADLQSSYNIIKKAIPEAFANGIEGIGLYPRSLSIRQMITSKGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q979K9</accession>
    <name>Q979K9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Archaeal chaperonin [group II]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1181974</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979K9"/>
    <dbReference type="STRING" id="273116.gene:9381951"/>
    <dbReference type="PaxDb" id="273116-14325390"/>
    <dbReference type="KEGG" id="tvo:TVG1181974"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="PhylomeDB" id="Q979K9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="526"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="549" mass="58788" checksum="1F45DFC1BB0493C8" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q979M1</accession>
    <name>Q979M1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH dehydrogenase I chain B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1165809</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979M1"/>
    <dbReference type="STRING" id="273116.gene:9381938"/>
    <dbReference type="PaxDb" id="273116-14325377"/>
    <dbReference type="KEGG" id="tvo:TVG1165809"/>
    <dbReference type="eggNOG" id="arCOG01554">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_2"/>
    <dbReference type="PhylomeDB" id="Q979M1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01150">
        <property type="entry name" value="COMPLEX1_20K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="3">
        <location>
            <begin position="13"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="116" mass="12804" checksum="A1A5D24DAB7B887B" modified="2001-10-01" version="1">MMAIQASDMDISRFGSEIFRNSPRQSDLMIVSGTVTKKMAPRVRRIYDEMPYPKYVIAQGVCVIQGGPYNQGYSVVLGVDKVVPVDVYVPGCPPTPEALTNGIILLQKKIRGEADR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-02" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q979P2</accession>
    <name>FLPA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">flpA</name>
        <name type="ordered locus">TV1118</name>
        <name type="ORF">TVG1150205</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917352.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979P2"/>
    <dbReference type="SMR" id="Q979P2"/>
    <dbReference type="STRING" id="273116.gene:9381917"/>
    <dbReference type="PaxDb" id="273116-14325356"/>
    <dbReference type="GeneID" id="1441234"/>
    <dbReference type="KEGG" id="tvo:TVG1150205"/>
    <dbReference type="eggNOG" id="arCOG00078">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059055_2_0_2"/>
    <dbReference type="OrthoDB" id="6244at2157"/>
    <dbReference type="PhylomeDB" id="Q979P2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000148552" description="Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="86"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="104"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="129"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00351"/>
        </source>
    </evidence>
    <sequence length="222" mass="25693" checksum="A54A0D3955C8912E" modified="2001-10-01" version="1">MSKGDRPGLLLPFENKRIIRIRNSVYTETETPQRVYGEEITKINNRTYREWDPRRSKPAAAILNGLRRFPIRENDSVLYLGASTGTTISHISDICPAGTIYGVEVSYEPFSKLLDLAKKRNNLYPILEDANSPEKYSFFIEHVDVMYQDISQRNQIQIFKNNVNTFNPKRGFLVLKIRSVKSTEDSRSILNTAMDQLSVYHLKEVINLKPYDTDHYLITLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Q2</accession>
    <name>Q979Q2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1140833 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1140833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917342.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Q2"/>
    <dbReference type="STRING" id="273116.gene:9381907"/>
    <dbReference type="PaxDb" id="273116-14325346"/>
    <dbReference type="GeneID" id="42990961"/>
    <dbReference type="KEGG" id="tvo:TVG1140833"/>
    <dbReference type="eggNOG" id="arCOG02466">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2784333_0_0_2"/>
    <dbReference type="OrthoDB" id="60264at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009633">
        <property type="entry name" value="PRK13149.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="68" mass="7656" checksum="A43DC912A2A4FB1E" modified="2001-10-01" version="1">MHSCKVVSVKGREMVVKLDGLMNIHTKVYDSKGQKIGYLIKVMGPVDESYGLVSLDKPDLQEDEIYIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-05" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Q5</accession>
    <name>SYP_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proline--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="1">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proS</name>
        <name type="ordered locus">TV1105</name>
        <name type="ORF">TVG1138861</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917339.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Q5"/>
    <dbReference type="SMR" id="Q979Q5"/>
    <dbReference type="STRING" id="273116.gene:9381903"/>
    <dbReference type="PaxDb" id="273116-14325343"/>
    <dbReference type="GeneID" id="1441221"/>
    <dbReference type="KEGG" id="tvo:TVG1138861"/>
    <dbReference type="eggNOG" id="arCOG00402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_4_2_2"/>
    <dbReference type="OrthoDB" id="7375at2157"/>
    <dbReference type="PhylomeDB" id="Q979Q5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000037">
        <property type="entry name" value="Proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000249177" description="Proline--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <sequence length="462" mass="53465" checksum="D1BD2B758CD3FD43" modified="2001-10-01" version="1">MENKKENFSEWYNEIVTISDLSDKRYPIKGMNVWRPYGWKIMKLIDNIIRNAVDKHSFDEVNFPVLISRGMLEVEFEHIRGFENEIYWVTKGGKEKLEEELALRPTSESAMYPMFSLWVRSHADLPLKIYQIVSVYRYETKHTRSFIRIREIHFFEAHTAHESYEDAEKQMDEYRIIWTEIADALCLPFLYDQRPEWDKFPGAMYTIAFDTVMPSGRSLQIGTIHQYGTNFSKNYDIKYLKEDGTFEYVHQTTFGMSERLLAAIIGIHGDDKGLILPPAIAPIQVVIVPIPGEGVERYAKDIETTLNGIGIRCHVDNRDNYTPGYKYNDWEMRGVPLRIEVGERELKEKTVTLAARNIRGKKTVQREKLVYEVPDMLDLVKEKITEDAKKTFNSLVVSASSLDDFKKEGLIKAFWCGSKECSDKIENETEKSALGFNLNNDETGKCIVCGKAGKLAIFSRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q979U3</accession>
    <name>Q979U3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1093558 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1093558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054016.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979U3"/>
    <dbReference type="STRING" id="273116.gene:9381862"/>
    <dbReference type="PaxDb" id="273116-14325305"/>
    <dbReference type="GeneID" id="1441178"/>
    <dbReference type="KEGG" id="tvo:TVG1093558"/>
    <dbReference type="HOGENOM" id="CLU_1048160_0_0_2"/>
    <dbReference type="OrthoDB" id="380737at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="265" mass="30337" checksum="20E4CA23D639ADDF" modified="2001-10-01" version="1">MIKISLIYIPGYIISSILASIVLEYMITHNPYRIKFQIIKSKKLVGSSLFSTRITNRDAIYLPNLQLYKISSPGFSSIISKELLDPTLVMFGTTRSDVDWKYLENAYFYDVFAFFYGVSLWFMVFLIMVPYNNLSDVLPKWDPVINYALLIVLSVGLLEASISYIFALYRNSYSLIYSLTLSVIAINATTFGPAMRWIDNIEIFIRITYQVVINILAVLIVIIVHSVNSVSYRYRLFAYTSSASYAAFATILLYSLVEKAMLLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Y0</accession>
    <name>Q979Y0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diphthamide synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1053893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Y0"/>
    <dbReference type="STRING" id="273116.gene:9381823"/>
    <dbReference type="PaxDb" id="273116-14325268"/>
    <dbReference type="KEGG" id="tvo:TVG1053893"/>
    <dbReference type="eggNOG" id="arCOG00035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010289_0_2_2"/>
    <dbReference type="OrthoDB" id="372052at2157"/>
    <dbReference type="PhylomeDB" id="Q979Y0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022427">
        <property type="entry name" value="MJ0570_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03679">
        <property type="entry name" value="arCOG00187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="205" mass="22880" checksum="A9B8E1426CEBFD5E" modified="2001-10-01" version="1">MSGGKDSFLSAVIAIEQGLELEKALIVKPSEYSYMFHFPNVLYATETASLLDLEYEFVDEAHFERKISELAGEGLNAIVSGAIASEYQKTRIERLCTANKIVSFTPLWKIDQERIIREIINAGIRAMIVSVSAEGLGEQDLGKEINLPYLDHLKSLEKRYGINISGEGGEYESFVFGYGSKVKRVQGHRVWSGSGGYFIIDRIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Y6</accession>
    <name>Q979Y6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1047273</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917252.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Y6"/>
    <dbReference type="STRING" id="273116.gene:9381817"/>
    <dbReference type="PaxDb" id="273116-14325262"/>
    <dbReference type="GeneID" id="1441134"/>
    <dbReference type="KEGG" id="tvo:TVG1047273"/>
    <dbReference type="eggNOG" id="arCOG00958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_6_2"/>
    <dbReference type="OrthoDB" id="15347at2157"/>
    <dbReference type="PhylomeDB" id="Q979Y6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF5">
        <property type="entry name" value="4FE-4S FERREDOXIN-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="70" mass="7782" checksum="D20CC2AF3CEBF987" modified="2001-10-01" version="1">MVELKVKTEMDVDRNLCNYCGACVGMCPTDAIWLDETVIKIHEEKCIECGFCIVGCPTGAINAEWFHGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Y9</accession>
    <name>Q979Y9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoate-protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1044624</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917249.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Y9"/>
    <dbReference type="STRING" id="273116.gene:9381814"/>
    <dbReference type="PaxDb" id="273116-14325259"/>
    <dbReference type="GeneID" id="1441131"/>
    <dbReference type="KEGG" id="tvo:TVG1044624"/>
    <dbReference type="eggNOG" id="arCOG01939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_0_0_2"/>
    <dbReference type="OrthoDB" id="43646at2157"/>
    <dbReference type="PhylomeDB" id="Q979Y9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16443">
        <property type="entry name" value="LplA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050664">
        <property type="entry name" value="Octanoyltrans_LipM/LipL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679:SF2">
        <property type="entry name" value="OCTANOYL-[GCVH]:PROTEIN N-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIPM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="30"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="262" mass="29938" checksum="6AF1484DB6278D32" modified="2001-10-01" version="1">MDGRLLFLETPGNTRMSLAYDESLYRSFKEGDKPILRFYRHDRSVIIGYFQIAEEEVDLDYMKRNNIMLARRYTGGGAVYHDLGDLNFSVITESHDMNITAMFRTMNEAVVRSLSYLGLQARPGELNDVSIPINKKTDIMAGEKKIMGAAGAMRKGSKLWHAAMLVKTDLDMLSAVLKVPDEKFRDKLAKSTRERVANVTDFVDVSIEDVEKSLIKGISEVLKIDFKEDTLRDDEENLAKDLYDKKYSTEEWNMGLLRKQVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-23" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Z3</accession>
    <name>SYS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA synthetase</fullName>
            <shortName evidence="1">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">serS</name>
        <name type="ordered locus">TV1017</name>
        <name type="ORF">TVG1039759</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Ser) + L-serine + ATP = L-seryl-tRNA(Ser) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Sec) + L-serine + ATP = L-seryl-tRNA(Sec) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917245.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Z3"/>
    <dbReference type="SMR" id="Q979Z3"/>
    <dbReference type="STRING" id="273116.gene:9381810"/>
    <dbReference type="PaxDb" id="273116-14325255"/>
    <dbReference type="GeneID" id="1441127"/>
    <dbReference type="KEGG" id="tvo:TVG1039759"/>
    <dbReference type="eggNOG" id="arCOG00403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_0_1_2"/>
    <dbReference type="OrthoDB" id="35932at2157"/>
    <dbReference type="PhylomeDB" id="Q979Z3"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000122188" description="Serine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="250"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="281"/>
            <end position="283"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="368"/>
            <end position="371"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <sequence length="441" mass="50948" checksum="F71ECBD37CAB2829" modified="2001-10-01" version="1">MIDVKLLRSNRELFEKNCEYRGVDKAIIDQFFKLDEEWRSVNRELNGQRAEKNRMTRAIAESLKRGKIVSNEKDRVELINEKIVSLEKKIREIEEERDRVLWTIPNLIHESVPICFGDENNKIVRYVGHAKVYRDDEEEFLKGSGGNGDYEVIDERPKSHVDLGQELGIIDLESAAKISGARFYFIKNRLLKLEMALENYAVDFLSQRGFTVVEPPYMLNLESMRGATDLETFKDTLYKIEDEDLYLIATSEHSIASMLSNEFLEEKELPIRVAGVSACFRREAGAHGKDTKGIFRVHQFNKIEQFIFCKPEDSWDYLEEILSNAEEIYRSLGIPYRVVNVCSGELGRLAAKKYDIEAWFPAQGKFREIVSASNDTDYQARSLNIKYRKSGGNEFVHTLNSTAIATTRILVAIMENFQEDGRIRIPDVLVPYTGFQYIDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A19</accession>
    <name>Q97A19_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GtrA/DPMS transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1012727</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917220.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A19"/>
    <dbReference type="STRING" id="273116.gene:9381783"/>
    <dbReference type="PaxDb" id="273116-14325208"/>
    <dbReference type="GeneID" id="1441102"/>
    <dbReference type="KEGG" id="tvo:TVG1012727"/>
    <dbReference type="eggNOG" id="arCOG02228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_881725_0_0_2"/>
    <dbReference type="OrthoDB" id="56015at2157"/>
    <dbReference type="PhylomeDB" id="Q97A19"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051401">
        <property type="entry name" value="GtrA_CellWall_Glycosyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA_DPMS_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38459">
        <property type="entry name" value="PROPHAGE BACTOPRENOL-LINKED GLUCOSE TRANSLOCASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38459:SF1">
        <property type="entry name" value="PROPHAGE BACTOPRENOL-LINKED GLUCOSE TRANSLOCASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA_DPMS_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA/DPMS transmembrane" evidence="7">
        <location>
            <begin position="187"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="315" mass="36086" checksum="45FF20BAE8527682" modified="2001-10-01" version="1">MLNNTQKAISEYYYDPLSILSDKTLIVITNDEDIPKNVPPFGEIKVFNRSAIEKDKQKFKSTLSESTYYMIVDGGYGSNAIENDVSLLDRYDIIVERRKLRWLLKIFKKVLDEDERLAETSLDVILTNSKVMEYIINRGYKISRETILKVISSNRFNVKYVDTNILQNVSSFFYVTLFEISHSSLLRYLAVGTSGIVVNEMLLKLLSYHIPLTFADALAIETSISTNFLLNEYWTFRGRKVSKSVKNIFKRMGLHNFTSLLGLGINLGIFTLLVYGGTEILIANMIGITAAFLSRYLMSSRIIWKNKNEKDIISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A39</accession>
    <name>Q97A39_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Molybdenum cofactor biosynthesis protein moeB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0993767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesA/MoeB/ThiF family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A39"/>
    <dbReference type="STRING" id="273116.gene:9381763"/>
    <dbReference type="PaxDb" id="273116-14325188"/>
    <dbReference type="KEGG" id="tvo:TVG0993767"/>
    <dbReference type="eggNOG" id="arCOG01676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_10_1_2"/>
    <dbReference type="PhylomeDB" id="Q97A39"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000080">
        <property type="entry name" value="Thiazole biosynthesis adenylyltransferase ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="2">
        <location>
            <begin position="52"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="305" mass="34031" checksum="4F92D889D70121EE" modified="2001-10-01" version="1">MLTGSFIFLRIAAFTTCYFQYSIKACPYKCQVAIYLSNGPILKAMLQDDYKYARQIALRVFNADDLKKIRNSVVSVIGVGGVGSLIADLFVRSGIKKLIIVDRDYVTSSNLYRQVLYDENDIGDSKAEAAKRRLSKVNSDVEIEARNETFDAGNAERIVSSSDLIMDGTDNLTSRLILNDAAVKNMKPWIMASAIETYGQVKAIIPEKTSCYRCYFSGEPFAQPTCAEVGVLSSLVTMVASIAWTLGIKVLTGKEVDGSLFNIDAWTQEIDRIAIDRMDDCECCVLKHFEYLSDRYKNIGLEFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A52</accession>
    <name>Q97A52_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0982439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917188.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A52"/>
    <dbReference type="STRING" id="273116.gene:9381750"/>
    <dbReference type="PaxDb" id="273116-14325175"/>
    <dbReference type="GeneID" id="1442036"/>
    <dbReference type="KEGG" id="tvo:TVG0982439"/>
    <dbReference type="eggNOG" id="arCOG00498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048478_0_0_2"/>
    <dbReference type="OrthoDB" id="205181at2157"/>
    <dbReference type="PhylomeDB" id="Q97A52"/>
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    <dbReference type="Gene3D" id="3.60.15.10">
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        <property type="entry name" value="Metallo-B-lactamas"/>
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    </dbReference>
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        <property type="entry name" value="Sec-metab_biosynth-thioest"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131:SF4">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY POTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
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    </dbReference>
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        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="312" mass="35405" checksum="03732C1B9CCD4DB0" modified="2001-10-01" version="1">MQTHVETFTVPIAIRALKTANIYRVPSDKGYILIDTGMSGDSVDQIVKESNDIQAVFLTHLHIDHVGGALRIHEELGVPVYMNSKDINLIRQVANDKEAYIKKYVDIFRGNGVPLSMQEDLIHMHPVINFYEYYSKLGFLQDISNLKLKELKFVPVPGHSPGSTAVITEDGYMMFSGDHILERITPNISVYGEEDDLGNYLKSLELVKKLSPKIIFPGHGSRIDDPLKRISEIEEHHAERIRAIAQALNESKTAFEIAESIPWSKGRKMDTMNFMEKNFAILETISHLRHMEAIGMVDSKDENGIIKYRIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A57</accession>
    <name>Q97A57_THEVO</name>
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            <fullName evidence="3">Aspartate kinase</fullName>
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        <name evidence="1" type="ORF">TVG0977291</name>
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    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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            <taxon>Thermoplasma</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A57"/>
    <dbReference type="STRING" id="273116.gene:9381745"/>
    <dbReference type="PaxDb" id="273116-14325170"/>
    <dbReference type="DNASU" id="1442032"/>
    <dbReference type="KEGG" id="tvo:TVG0977291"/>
    <dbReference type="eggNOG" id="arCOG01806">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399799_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97A57"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="22843" checksum="C26342931955AC3E" modified="2001-10-01" version="1">MISEIMKRSGKGDVERKVLEYVSLRPEVYLTFRDGLLNLNALAKMISEKYTNLNPLSVRASLQKLLQQAGFKDYRKYTDDLLRKSKIVLQDKVTVITSRHKVDVDYLSATFLTDSVVYIIDESRTKTVPKDVQVQRDVSLIHIFSPPEIVSTPGFALNVVERVYSMGINIIQLISCSNETMIVLKREDSIPAYKSLTDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A67</accession>
    <name>Q97A67_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Precorrin-4 c11-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0970612</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="273116.gene:9381735"/>
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    <dbReference type="eggNOG" id="arCOG00645">
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    <dbReference type="HOGENOM" id="CLU_011276_7_1_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF4">
        <property type="entry name" value="COBALT-PRECORRIN-4 C(11)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="168" mass="18631" checksum="7AF1755248AC535E" modified="2001-10-01" version="1">MYGALMEQLSLLREKGIDFEVIPGVSVLTSAAASLGIELTMDNISQSVIIARGSLRIPVPEKQSILALSSHQSTMVIFTAIHIIEKVVEDLLKGGYPKDTPVGVVYHSTWKDEKVIRGTLEDIVQKVKDNRIYRTAIIVVGKVVNPERVYSTKLYDPSYTHSFRRGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A74</accession>
    <name>Q97A74_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0963330 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0963330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A74"/>
    <dbReference type="STRING" id="273116.gene:9381728"/>
    <dbReference type="PaxDb" id="273116-14325153"/>
    <dbReference type="KEGG" id="tvo:TVG0963330"/>
    <dbReference type="HOGENOM" id="CLU_3113288_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="50" mass="5838" checksum="A29956445963915C" modified="2001-10-01" version="1">MPCQHLAHNKKLIYLNILLTYISGSNSFSYTSRRENGVKNILWNDEIIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AD3</accession>
    <name>Q97AD3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0893971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AD3"/>
    <dbReference type="STRING" id="273116.gene:9381669"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
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    <dbReference type="DNASU" id="1441969"/>
    <dbReference type="GeneID" id="1441969"/>
    <dbReference type="KEGG" id="tvo:TVG0893971"/>
    <dbReference type="eggNOG" id="arCOG07390">
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="186"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="377" mass="43032" checksum="F9366511627D193E" modified="2001-10-01" version="1">MKIALFTNTNVSLGAGIEKTLLNYVKYNTDHQLVIVQSQFYKVQRLPNDVLDRIGIKNVVTIKDYEHCISFLRMHRITKPIYALILPLLIRITKVLNIKKLRSIGELDVVYLFKNEYWPLFKGKLIIGSNHAQFAFDNTYNRILALLVRSGLVYRGIDAFHLFPKSKGIGKLMGKRYFTGIIGVETSQFKPKARDNTVNVLFVGRLEYIKGIDLFIEVCKKFEGNTGVKFNVSGGGEYSQILNDNKLPNLTYHGVLSEGELADLYGKCDIFLYPTRWDAQPSVIAEALSAGEYIITSQRIRGTYDDLSEKGFLEYSSLSLDDLSNRLLKAIDRVAELRAISDAEHSYAVEKYDNSVVVRDLYKNIENLYISMQRNQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AD5</accession>
    <name>Q97AD5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Prephenate dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0891777</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917119.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AD5"/>
    <dbReference type="STRING" id="273116.gene:9381667"/>
    <dbReference type="PaxDb" id="273116-14325092"/>
    <dbReference type="GeneID" id="1441967"/>
    <dbReference type="KEGG" id="tvo:TVG0891777"/>
    <dbReference type="eggNOG" id="arCOG00255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035008_0_2_2"/>
    <dbReference type="OrthoDB" id="8755at2157"/>
    <dbReference type="PhylomeDB" id="Q97AD5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13532">
        <property type="entry name" value="PBP2_PDT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008865">
        <property type="entry name" value="PRK11898.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022">
        <property type="entry name" value="PREPHENATE DEHYDRATASE P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF19">
        <property type="entry name" value="PREPHENATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="185"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="268" mass="29718" checksum="3B3A9BE006968520" modified="2001-10-01" version="1">MSTGYLGPKMSYSFLATSKMGYNDGVPFKTIFDLFDAVEKGGIDRAVVPVENSLEGQVGQTLDLLFSKDVSIIEEYYLRVVHCIAATDNADVKIVHSHPQALMQCSNYIEKHGYAVIEETSTTAGIEKLFNEQNKGHAVICSEEAASACKLTILDRDIGNEHDNFTRFFVITKKGLAKPQGDKISIAFTLLNKPGSLASIVDVLAKYGIDMTKIESRPIKFNPFSYIFYIDFIDNGFSKQAMDEISQKTVSLKILGKYRKAIGPVMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AF2</accession>
    <name>Q97AF2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0878825 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0878825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AF2"/>
    <dbReference type="STRING" id="273116.gene:9381650"/>
    <dbReference type="PaxDb" id="273116-14325075"/>
    <dbReference type="GeneID" id="1441950"/>
    <dbReference type="KEGG" id="tvo:TVG0878825"/>
    <dbReference type="eggNOG" id="arCOG02682">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2821130_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="66" mass="7685" checksum="DD5248B78115D69B" modified="2001-10-01" version="1">MKQNALLKTLDNIDVRRYHYFITILSDMGYFLDGYDLLVIGPALLFIGPLFNIHALTKTRDKPRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AF6</accession>
    <name>Q97AF6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Anaerobic glycerol-3-phosphate dehydrogenase subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0875243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917098.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AF6"/>
    <dbReference type="SMR" id="Q97AF6"/>
    <dbReference type="STRING" id="273116.gene:9381646"/>
    <dbReference type="PaxDb" id="273116-14325071"/>
    <dbReference type="DNASU" id="1441946"/>
    <dbReference type="GeneID" id="1441946"/>
    <dbReference type="KEGG" id="tvo:TVG0875243"/>
    <dbReference type="eggNOG" id="arCOG00753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015740_0_1_2"/>
    <dbReference type="OrthoDB" id="36306at2157"/>
    <dbReference type="PhylomeDB" id="Q97AF6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985:SF15">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="3"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="414" mass="45301" checksum="987EE5C23929E629" modified="2001-10-01" version="1">MEKIAVVGAGITGLFTAYYLSASGFDVEVFDKGDIASGTSGKFHGMLHSGARYAVTDKVSASECISENNTISSFARWFINDTGGYFVSSNREESDYGDTLTKACNDAGIPNYDIDVDEFKKIEPNVKNVDRVIHVPDKVIRAYEFSAAVAAEAFMNGTKFRLYSPVASVSEKGGKYVLGIERNGSLHYEHFDLIVNATGPFSGEFLKLAGYGGSPVMPSAGIMVVFSKPIVRSIINRMREPSDADIMVPYDGLSILGTSAVIVDDPENFSIDSEDVEAMIEDMSSLIPLIRNVNYSRTYYSVRPLLEDEGDDARKASRSFRIIKNGEGVFTVVGGKFTTGRLIGEHVAKEISKETGATIRIENPDLNSTYERFIEKYGRDDKYIAKAMSRIGTIDEENAMRSVAYAISTIIEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AI1</accession>
    <name>Q97AI1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SAM-dependent methyltransferase TRM5/TYW2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0844590</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917073.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AI1"/>
    <dbReference type="STRING" id="273116.gene:9381621"/>
    <dbReference type="PaxDb" id="273116-14325045"/>
    <dbReference type="GeneID" id="1441921"/>
    <dbReference type="KEGG" id="tvo:TVG0844590"/>
    <dbReference type="eggNOG" id="arCOG00033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_0_1_2"/>
    <dbReference type="OrthoDB" id="8079at2157"/>
    <dbReference type="PhylomeDB" id="Q97AI1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040601">
        <property type="entry name" value="Trm5a/b_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18093">
        <property type="entry name" value="Trm5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="6">
        <location>
            <begin position="70"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="307" mass="34761" checksum="C3002B5E3E7181DE" modified="2001-10-01" version="1">MSLKGFVKVERRMADRTIKMLQSRGYFDSDYEIRHEGDYVLIPVKDGFTGPVVVKDAEKKPKVIPASGSFDIIGTIAITKKDDVEFAKEILKTHKNIKSVYLDKGVKGKERIRDLVLLAGIDSTVALYRENGCIFKIDVSKSYFSPRLATERRRLVDSILDGEFIVDMFAGSGPISIEVAKKRRVKIISIDINCAAIESLQESMRLNSLLGTITPICGDARQLIKDIHDADRIIMNHPTDAFSFLPCALEALKYGGHINYYEILNKDDVTKRLADFNFMRLKVFSYHRVHSYSKLSDLISFDLIREG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-03" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AK6</accession>
    <name>THII_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA sulfurtransferase</fullName>
            <ecNumber evidence="1">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiI</name>
        <name type="ordered locus">TV0804</name>
        <name type="ORF">TVG0805746</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + ATP + H(+) = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + 2 oxidized [2Fe-2S]-[ferredoxin] + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[ThiS sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [ThiS sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="Rhea" id="RHEA-COMP:19908"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:232372"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiI family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917048.1">
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="174"/>
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        </location>
        <ligand>
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            <begin position="334"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <sequence length="476" mass="54073" checksum="D714D9C7461DDE60" modified="2001-10-01" version="1">MKTYLVRYSEIGLKGDRERARMERILADNIINYYKKIGYEARCQLLAGRLLVEAENDIPLSKVFGIKSYSECIRIKFENQEDIVKKVHALYEEKVKGKTFGVRCRRTGTHSFTSIDMEKAIGDALYSISNGVDLKSPEVWIHVDIVGKDALIYDKIYKGPGGLPLGSEGKLISMVSGGIDSPVATWLMMKRGSPCDIFFCSLADPIDTQAFLEIAKKLVERWSPYRDGNVFIADCRDLIRDMVIEKKTNFNNVTFKKVLYRLAERLAEKYRYLGIVTGESLGQVSSQTAENLLSIEHGINFPIYRPLIGLDKDEITAIARDIGTFPEKNVGEFCSLFSAHPVTRSKWEDIEEDVKKIDIEKFIERVTAIKFSEIGKIVINSDLMLNKNLEDAVFIDLRKKDLYEKSHYQGARHLDLEQALAINDTSKKYVFYCSMGLQSAYVASVLRERGIEAYFTTFSKLSKQKGSVDETIGKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AK9</accession>
    <name>Q97AK9_THEVO</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0800944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="tvo:TVG0800944"/>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
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        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
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        <location>
            <begin position="119"/>
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        </location>
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        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
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        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
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        <location>
            <begin position="331"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="374"/>
        </location>
    </feature>
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        <location>
            <begin position="380"/>
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        </location>
    </feature>
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        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="476" mass="53272" checksum="ABE8C8FC062B4CBA" modified="2001-10-01" version="1">MCLAIVMSYLLAYLYDKMGYYSIKNNLETFGGPSFIRSAHGRSSLRYFISRFSMWIANTSLASFSVIYFVDFTFTVIDPLVQSIGFSQLDIDLLSAAIIIFMVVWFIINAFYGKRFIRGIGYTQIGFLIIMVSIIFTDTLDLGFKFSWNLSGLSSFHGNLASAIIMNTGYLFILFFGYQEIQVMERDSKEEGSIPILSQIKHKKYPKTKYLPASMYATIAAAGIIQILYGLAVFSLHANTSSVEQAVIPAIYLASEFQNKMWALMMAFSFLLATVTTFVPAFMAASRHLRSLSEDGIFPRPLASLSWVFTFILILFMSFGGTAFLVNITDFMVLVSLSFVALSAMVLKENSKFDRLSVISLITFLMFMISAISIYMIDRIVVVFGVIALIASFLLYDILRLKLFGLEVLGAFFGTLSLLSTLVMKSSASPSVLIIGNIAVRSPYNLLFLQISVLVLVVILLANAITRYHIERKAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AP2</accession>
    <name>Q97AP2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Folylpolyglutamate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0771612</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AP2"/>
    <dbReference type="STRING" id="273116.gene:9381558"/>
    <dbReference type="PaxDb" id="273116-14324984"/>
    <dbReference type="KEGG" id="tvo:TVG0771612"/>
    <dbReference type="eggNOG" id="arCOG02817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015869_1_2_2"/>
    <dbReference type="PhylomeDB" id="Q97AP2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase central" evidence="8">
        <location>
            <begin position="21"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Mur ligase C-terminal" evidence="7">
        <location>
            <begin position="267"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="407" mass="45883" checksum="29A4955157F11760" modified="2001-10-01" version="1">MMREFADMLGHPERDFRSIHITGSNGKGSTSAFIYSILRQKFSVGVYTSPHLERFNERIIVDNEEVPDEYIDGFIEKYRPKFDELSKVNRNPTFFEVTTMMAFQYFKDRKVDYASVEVGLGGRLDATNIIMPEVSVIAQVGYEHADKLGCSLTSIAYEKAGIIKEGRPIVLQDDKPEVVNEVRRIAQVKHSPLITLDRNKIKNLHVDENGTDFDFDGTNDTYHIHTSLIGDYQALNAATAILAVENSKVQVYKKDIETGISRTIWPGRLEIIDRSPLVIIDAAHNPPAANKMVRTVKKVFNREPTIVAGILSDKDAYSFFSVLRQLSGKIIFTTPEEKERAVDPNILMAQYGNLFRSARVIPDPISAFEHAKKVDDFVFVTGSIYLIGVIRGLYDKANEKKGTKIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AR0</accession>
    <name>Q97AR0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroneopterin aldolase</fullName>
            <shortName evidence="1">DHNA</shortName>
            <ecNumber evidence="1">4.1.2.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7,8-dihydroneopterin aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mptD</name>
        <name evidence="3" type="ORF">TVG0753807</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 7,8-dihydroneopterin (H2Neo) to 6-hydroxymethyl-7,8-dihydropterin (6-HMD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal dihydroneopterin aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916996.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AR0"/>
    <dbReference type="STRING" id="273116.gene:9381539"/>
    <dbReference type="PaxDb" id="273116-14324965"/>
    <dbReference type="GeneID" id="1441844"/>
    <dbReference type="KEGG" id="tvo:TVG0753807"/>
    <dbReference type="eggNOG" id="arCOG04705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149105_1_0_2"/>
    <dbReference type="OrthoDB" id="132689at2157"/>
    <dbReference type="PhylomeDB" id="Q97AR0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.20">
        <property type="entry name" value="7,8-dihydroneopterin aldolase (MptD)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02130">
        <property type="entry name" value="DHNA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027508">
        <property type="entry name" value="DHN_aldolase_MptD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036839">
        <property type="entry name" value="MptD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007181">
        <property type="entry name" value="MtpD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04038">
        <property type="entry name" value="DHNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143560">
        <property type="entry name" value="MK0786-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroneopterin aldolase MtpD C-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="120" mass="13723" checksum="B35E6D1EF51D954D" modified="2001-10-01" version="1">MKDPAAHYFKCTSRERAIFEAGIKLGTVYHQYVGIPLNQSNISSLEKAIRDSIMVQPFVVEAEVKIDPNVNNKKPGVYKYLTLSGEMLDVMVKVDYEGSVVKARLRYVPEMDYPLMYIEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AU2</accession>
    <name>RPIA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="1">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoriboisomerase A</fullName>
            <shortName evidence="1">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpiA</name>
        <name type="ordered locus">TV0717</name>
        <name type="ORF">TVG0723257</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916975.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AU2"/>
    <dbReference type="SMR" id="Q97AU2"/>
    <dbReference type="STRING" id="273116.gene:9381506"/>
    <dbReference type="PaxDb" id="273116-14324933"/>
    <dbReference type="GeneID" id="1441823"/>
    <dbReference type="KEGG" id="tvo:TVG0723257"/>
    <dbReference type="eggNOG" id="arCOG01122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056590_1_0_2"/>
    <dbReference type="OrthoDB" id="19013at2157"/>
    <dbReference type="PhylomeDB" id="Q97AU2"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000001">
        <property type="entry name" value="Ribose-5-phosphate isomerase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001924">
        <property type="entry name" value="PRK00702.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000158520" description="Ribose-5-phosphate isomerase A">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="84"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="97"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <sequence length="241" mass="26734" checksum="667F05C6BBE644D2" modified="2001-10-01" version="1">MPDYEKQKRNAAVEAASYVKTGMVIGIGTGTTARYLIEELGRRVSEENLKIKGVCTSKKSEELARAAGIEISENPEQIIDLTIDGADQVNLHGTLIKGGGGALLREKIVAYNSKEMYVIVDSRKIDDENFGSFPLPVEVVPFLHRMTLENLRKICPSTDLRKNSDGTLFITDNGNYIADMKFGRIRETNELEKKIKSIPGVVDVGLFNNIADKIFEGNDDGCNIYVVSEKGRIEKEKGQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AV9</accession>
    <name>Q97AV9_THEVO</name>
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        <submittedName>
            <fullName evidence="1">TVG0706547 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0706547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_156769072.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AV9"/>
    <dbReference type="PaxDb" id="273116-14324916"/>
    <dbReference type="GeneID" id="42990947"/>
    <dbReference type="KEGG" id="tvo:TVG0706547"/>
    <dbReference type="HOGENOM" id="CLU_3038886_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="60" mass="6798" checksum="75FBD0F07185421F" modified="2001-10-01" version="1">MTEISLARIKAAAMMMPEPVKSLILSEPDTLDASELITKLGTWDRLLKIEKAENTKNRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AX4</accession>
    <name>Q97AX4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Beta-glycosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0691226</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916943.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AX4"/>
    <dbReference type="SMR" id="Q97AX4"/>
    <dbReference type="STRING" id="273116.gene:9381473"/>
    <dbReference type="CAZy" id="GH1">
        <property type="family name" value="Glycoside Hydrolase Family 1"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324901"/>
    <dbReference type="GeneID" id="1441791"/>
    <dbReference type="KEGG" id="tvo:TVG0691226"/>
    <dbReference type="eggNOG" id="arCOG05412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001859_1_3_2"/>
    <dbReference type="OrthoDB" id="84443at2157"/>
    <dbReference type="PhylomeDB" id="Q97AX4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053427">
        <property type="entry name" value="Beta-galactosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041004">
        <property type="entry name" value="Beta_gal_BgaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF209">
        <property type="entry name" value="GALACTOLIPID GALACTOSYLTRANSFERASE SFR2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="481" mass="55192" checksum="89800E0AABF174A8" modified="2001-10-01" version="1">MVENNFPEDFKFGWSQSGFQSEMGYDNAMDDKSDWYVWVHDKENIQSGLVSGDMPENGPGYWNNYKSFHEAAQNMGLKMARIGVEWSRLFPEPFPEKIMADAKNNSLEINNNILSELDKYVNKDALNHYIEIFNDIKNRNIDLIINMYHWPLPVWLSDPVSVRKGIKTERSGWLNDRIVQLFALFSSYIVYKMEDLAVAFSTMNEPNVVYGNGFINIKSGFPPSYLSSEFASKVKNNILKAHSLAYDSMKKITDKPVGIIYANTYFTPLDPEKDNDAIAKADSDAKWSFFDPLIKGDKSLGINGNKLDWIGINYYTRTMLRKDGDGYISLKGYGHSGSPNTVTNDKRPTSDIGWEFYPEGLEYVIMNYWNRYKLPMYVTENGIADNGDYQRPYYLVSHIASVLRAINKGANVKGYLHWSLVDNYEWALGFSPKFGLIGYDENKKLYWRPSALVYKEIATKNCISPELKHLDSIPPINGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B22</accession>
    <name>Q97B22_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0629505 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0629505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B22"/>
    <dbReference type="STRING" id="273116.gene:9381425"/>
    <dbReference type="PaxDb" id="273116-14324853"/>
    <dbReference type="KEGG" id="tvo:TVG0629505"/>
    <dbReference type="eggNOG" id="arCOG15086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2504811_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="93" mass="10129" checksum="648B57D00890CDCB" modified="2001-10-01" version="1">MVSIPRIPITYIASYGTSISSILSRLDTIVLSVSAVIISILWIPVALEFFSSDESKRISARTRLKNAAIGTFIYIMAVSGILYALVKYIITGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B48</accession>
    <name>Q97B48_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Flagella-related protein G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0600631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916869.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B48"/>
    <dbReference type="STRING" id="273116.gene:9381399"/>
    <dbReference type="PaxDb" id="273116-14324826"/>
    <dbReference type="GeneID" id="1441717"/>
    <dbReference type="KEGG" id="tvo:TVG0600631"/>
    <dbReference type="eggNOG" id="arCOG01822">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134827_1_0_2"/>
    <dbReference type="OrthoDB" id="183655at2157"/>
    <dbReference type="PhylomeDB" id="Q97B48"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200">
        <property type="entry name" value="ARCHAEAL FLAGELLA-RELATED PROTEIN F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200:SF1">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0966">Cell projection</keyword>
    <keyword evidence="1" id="KW-0969">Cilium</keyword>
    <keyword evidence="1" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="147" mass="15775" checksum="BFE1B2C81CDC61A5" modified="2001-10-01" version="1">MASTAVSELVFFIVTLLITASAVAVLSDQTIHIVYGMQSSSQRTTDMIQENFAIINNPDQIPYDNGYVFYIKNTGSVGFAFDNTSISVLVDGSAVTGKYVKFISLENSSILVPGQVGEIVVNETLSGYNSITISLGTGVSHTFNFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B50</accession>
    <name>Q97B50_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Archaeal flagella protein FlaD/E domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0599784</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916867.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B50"/>
    <dbReference type="STRING" id="273116.gene:9381397"/>
    <dbReference type="PaxDb" id="273116-14324824"/>
    <dbReference type="GeneID" id="1441715"/>
    <dbReference type="KEGG" id="tvo:TVG0599784"/>
    <dbReference type="eggNOG" id="arCOG02964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047519_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97B50"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006752">
        <property type="entry name" value="Arch_fla_DE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016682">
        <property type="entry name" value="FlaD_prd_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052494">
        <property type="entry name" value="Flagella_assembly_related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40698:SF1">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40698">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN E-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04659">
        <property type="entry name" value="Arch_fla_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017066">
        <property type="entry name" value="FlaD_arch_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal flagella protein FlaD/E" evidence="3">
        <location>
            <begin position="98"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="217" mass="24853" checksum="7E027F04A3FFAA47" modified="2001-10-01" version="1">MINDALKPYLEEKIESAKKTVPSFALEELKQKIISSPIQFGKSDIDDLVANLLKRYEQTKENSLIGKIDSIFKEISDMEKVMSGKAAEPAQPAEKPKKKAKLEAIGDDVVSIMVSLRWLEFLLDNYGPENTLDVLDYYESLGWISSTVKEQMMKYAKMTGVMNPAQEYKIKPSIQDHIINMLFIEKLRGEEINRDLIEALEREFRTIRKGVDELYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B69</accession>
    <name>Q97B69_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cysteine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0578372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916847.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B69"/>
    <dbReference type="STRING" id="273116.gene:9381377"/>
    <dbReference type="PaxDb" id="273116-14324804"/>
    <dbReference type="GeneID" id="1441695"/>
    <dbReference type="KEGG" id="tvo:TVG0578372"/>
    <dbReference type="eggNOG" id="arCOG01430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q97B69"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="1">
        <location>
            <begin position="22"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="309" mass="33766" checksum="D16D753BC957269E" modified="2001-10-01" version="1">MMHAELGPDMVSKILDHMKAAHIGNTPVEEIDANGNIYAKIEWENRFGSIKDRPAFFIMDYLKSKGVLEGKAIVEASSGNTGLAISTISSMLGLQSCIVLPENASQATKKIIVENGSQLILTPPDLGTEGSIEKARSLASSGSFYWINQHSNPLNSDVHYYSTAPELLDQEGMPEAIVAGVGTGGTITGIARYFKERDPSVKVIGVQPSLYSHIPGLRDIKKTSNKGLIDLYGSYIDDFLYVKESDAIAEVFDHYVNTGELIGISSGANLYASKQLSSKFKKIFTVFPDSGRKYLDILKPYDKNNVLNY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B83</accession>
    <name>RIBA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP cyclohydrolase-2</fullName>
            <ecNumber evidence="1">3.5.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP cyclohydrolase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ribA</name>
        <name type="ordered locus">TV0574</name>
        <name type="ORF">TVG0563044</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 2 phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916833.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B83"/>
    <dbReference type="SMR" id="Q97B83"/>
    <dbReference type="STRING" id="273116.gene:9381360"/>
    <dbReference type="PaxDb" id="273116-14324789"/>
    <dbReference type="GeneID" id="1441091"/>
    <dbReference type="KEGG" id="tvo:TVG0563044"/>
    <dbReference type="eggNOG" id="arCOG01321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_2_1_2"/>
    <dbReference type="OrthoDB" id="25735at2157"/>
    <dbReference type="PhylomeDB" id="Q97B83"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10990:FF:000002">
        <property type="entry name" value="GTP cyclohydrolase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="entry name" value="GTP cyclohydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00179">
        <property type="entry name" value="RibA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001591">
        <property type="entry name" value="PRK00393.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00505">
        <property type="entry name" value="ribA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF18">
        <property type="entry name" value="3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="RibA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000071649" description="GTP cyclohydrolase-2">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00179"/>
        </source>
    </evidence>
    <sequence length="195" mass="22328" checksum="A72FBBE6C700824B" modified="2001-10-01" version="1">MIELYSNAKLPTEFGLFRIYTFVNDENKDHAVIVRGDPRGKENVPLRIHSECLTGDVLGSLRCDCRDQLIQSLRYLGRQEYGMLIYLRQEGRGIGLLNKIKAYSLQDMGADTVEANLKLGLPVDSRNYSFAAEVLRYFEIKSIYIMTNNPEKIKSLIESGINVKGRIPIFSDPTPYDKFYLETKKTKLGHEIENI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BA1</accession>
    <name>PRIL_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase large subunit PriL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">priL</name>
        <name type="synonym">priB</name>
        <name type="ordered locus">TV0556</name>
        <name type="ORF">TVG0544022</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916815.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BA1"/>
    <dbReference type="STRING" id="273116.gene:9381341"/>
    <dbReference type="PaxDb" id="273116-14324771"/>
    <dbReference type="DNASU" id="1441073"/>
    <dbReference type="GeneID" id="1441073"/>
    <dbReference type="KEGG" id="tvo:TVG0544022"/>
    <dbReference type="eggNOG" id="arCOG03013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052778_0_0_2"/>
    <dbReference type="OrthoDB" id="46081at2157"/>
    <dbReference type="PhylomeDB" id="Q97BA1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06560">
        <property type="entry name" value="PriL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00701">
        <property type="entry name" value="DNA_primase_lrg_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023642">
        <property type="entry name" value="DNA_primase_lsu_PriL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002589">
        <property type="entry name" value="PRK02249.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537:SF3">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26466">
        <property type="entry name" value="DNA_primase_lrg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140914">
        <property type="entry name" value="PriB N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000046790" description="DNA primase large subunit PriL">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00701"/>
        </source>
    </evidence>
    <sequence length="327" mass="37915" checksum="55A1A467BAAC1645" modified="2001-10-01" version="1">MRFPPLLFFQDSEAVKRIANSVRGTKDDDAEIKDHSMRFIRNAISGDQQKEILNVDMLRYIAWVLVALNENIVTARTVIRERDAVEDALKNQQPEDIENFADDLKINMKYNRDLERFEIGVFDFVKYASRVTGSQYRLSNQSVIKGIVYCQKDVAIKILRESFVSNMFKVIDSIDQTTASPVLSDQTDSINELREFYKKSVYAKLTIGRGNTKAFPPCMKEIIRNLHEGINVPHMGRLAIASFLHKVGYTEDEIVEYFRNAPDFDESITRYQIKHLSGEISGVEYSPPKCETMRSNHLCFMDDDKLCHQDWMKHPLTYYEVKSRRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BB2</accession>
    <name>Q97BB2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC transport system permease protein PA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG0536211</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BB2"/>
    <dbReference type="STRING" id="273116.gene:9381330"/>
    <dbReference type="PaxDb" id="273116-14324760"/>
    <dbReference type="KEGG" id="tvo:TVG0536211"/>
    <dbReference type="eggNOG" id="arCOG00164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_1_2"/>
    <dbReference type="PhylomeDB" id="Q97BB2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30183">
        <property type="entry name" value="MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30183:SF3">
        <property type="entry name" value="MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="35"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="251" mass="27527" checksum="D5FE5EFB41BE4F89" modified="2001-10-01" version="1">MLYILIILVPIIRALTYSSIGEITADLSSGILPSIEYTYAICGVVAFLAVLFSLPYSYVISRHTGPAYKAADSLVEIPIMIPHSVVGIIMLITFEPTMPIGHLLAKYIPGYSFDGTLFAVIITLFFLSSAYSIREIGIAYQKDVMEYENVAKTLGLGETLSFVIISMRLLLRPMMKGFLLSWARSVSEVGAILIVAYYIFPSFVKLAGVFIYSQWLGYGLGPAAASSAILIITGIAVTGIFKMVESYHVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BB4</accession>
    <name>Q97BB4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Isochorismatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0534221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BB4"/>
    <dbReference type="STRING" id="273116.gene:9381328"/>
    <dbReference type="PaxDb" id="273116-14324758"/>
    <dbReference type="GeneID" id="1441059"/>
    <dbReference type="KEGG" id="tvo:TVG0534221"/>
    <dbReference type="eggNOG" id="arCOG01943">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_8_3_2"/>
    <dbReference type="OrthoDB" id="9194at2157"/>
    <dbReference type="PhylomeDB" id="Q97BB4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Isochorismatase-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF6">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="2">
        <location>
            <begin position="18"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="208" mass="23005" checksum="E6BFFB441F65F55B" modified="2001-10-01" version="1">MVARLYRELKEIVEPSHTVLVAWDVQNGLVNSIFNKNEFLGVVKNVIQSARKKGVKVIYTKITPMPPGFESGYRLYSMMKRYGVSDPDKVRFMEPGSPEAEIYSELAPEQGDIVINKNTADLFIGTNVELMLRNAGIDTILFTGIATEFGIESSARSAGNRGFYPVVIQDGVSSPNKDMHEAALKILATQAITIKSEDLMSAWDTAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BE2</accession>
    <name>Q97BE2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0502242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BE2"/>
    <dbReference type="STRING" id="273116.gene:9381297"/>
    <dbReference type="PaxDb" id="273116-14324729"/>
    <dbReference type="DNASU" id="1441030"/>
    <dbReference type="GeneID" id="1441030"/>
    <dbReference type="KEGG" id="tvo:TVG0502242"/>
    <dbReference type="eggNOG" id="arCOG04060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077869_0_0_2"/>
    <dbReference type="OrthoDB" id="371772at2157"/>
    <dbReference type="PhylomeDB" id="Q97BE2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017271">
        <property type="entry name" value="Tscrpt_reg_HTH_MJ1545_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037724">
        <property type="entry name" value="TF_HTH_MJ1545_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="226" mass="25610" checksum="F216493E0F6BCA74" modified="2001-10-01" version="1">MDIELREKIAGEITLSDSPAETIRKWREEFKVSQLELSNFLNQSPSVISDYETGRRKFPSIASVRKIVDALITIDERRGGYVIKKYKSGVPSDALIDIKDYDRDVPLEKAIRAMNGKNVSHVSVERNIRGYTIVDGVKAILAFSYYEYSKLYGWSSERAIFFTDVKFGRSPMIAIRVHPLKPAAVVYIQPDNVDDLAIKLADIENIPLLITEMSPEAVSHVMSQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BF3</accession>
    <name>Q97BF3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0491515 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0491515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916761.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BF3"/>
    <dbReference type="PaxDb" id="273116-14324718"/>
    <dbReference type="GeneID" id="1441019"/>
    <dbReference type="KEGG" id="tvo:TVG0491515"/>
    <dbReference type="eggNOG" id="arCOG05325">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1640047_0_0_2"/>
    <dbReference type="OrthoDB" id="56540at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="164" mass="18635" checksum="D162B9B22A1DC719" modified="2001-10-01" version="1">MRIKTISIYDGTVDRDAVKSLLGESTIMVLDMDAIQHHTLNVSEYTYLSHFFSIVVVNLARKLNDLIDSIMAGSSMVVISPWVKERVLRDFLDVSENVVMPYVNVALLHVYQDMGGQYYLVEYPIDKPFKTAFYLGVPPDDTYTQVDGFPDSVKNIIFRDSANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BF8</accession>
    <name>Q97BF8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">tRNA carboxymethyluridine synthase</fullName>
            <ecNumber evidence="13">2.3.1.311</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">TVG0485350</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.311" evidence="13"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BF8"/>
    <dbReference type="STRING" id="273116.gene:9381281"/>
    <dbReference type="PaxDb" id="273116-14324713"/>
    <dbReference type="DNASU" id="1441014"/>
    <dbReference type="KEGG" id="tvo:TVG0485350"/>
    <dbReference type="eggNOG" id="arCOG01361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025983_2_1_2"/>
    <dbReference type="PhylomeDB" id="Q97BF8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF7">
        <property type="entry name" value="TRNA URIDINE(34) ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 15" id="KW-0408">Iron</keyword>
    <keyword evidence="11 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="16">
        <location>
            <begin position="82"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAB59640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="531" mass="61170" checksum="300097F959AC3884" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BF9</accession>
    <name>Q97BF9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adenosine monophosphate-protein transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0483190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916755.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BF9"/>
    <dbReference type="PaxDb" id="273116-14324712"/>
    <dbReference type="GeneID" id="1441013"/>
    <dbReference type="KEGG" id="tvo:TVG0483190"/>
    <dbReference type="eggNOG" id="arCOG04298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138382_0_0_2"/>
    <dbReference type="OrthoDB" id="371841at2157"/>
    <dbReference type="PhylomeDB" id="Q97BF9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.30">
        <property type="entry name" value="Ta1353-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1520.10">
        <property type="entry name" value="Ta1353-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007153">
        <property type="entry name" value="Adenosine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036902">
        <property type="entry name" value="Ta1353-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36155">
        <property type="entry name" value="BLL5354 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36155:SF1">
        <property type="entry name" value="BLL5354 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04008">
        <property type="entry name" value="Adenosine_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103165">
        <property type="entry name" value="Ta1353-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="17628" checksum="5EF617CA93B58B83" modified="2001-10-01" version="1">MDIEAVDMIVPNDSNIIVGYSHFIKTVEDLNEIVHTSMPKCRYAIAFSEASGDRLIRYEGNDDELVNAAIENIKRISAGHTFVILLRDAYPINILNAVKSCQEVGGIFAATANSLKIIIYKGENGNGVLGVIDGMSPVGVESDDDKKKRRDLLRKIGYKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BG8</accession>
    <name>DNAK_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaK</name>
        <name type="ordered locus">TV0487</name>
        <name type="ORF">TVG0471466</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a chaperone.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054084.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BG8"/>
    <dbReference type="SMR" id="Q97BG8"/>
    <dbReference type="STRING" id="273116.gene:9381269"/>
    <dbReference type="PaxDb" id="273116-14324702"/>
    <dbReference type="GeneID" id="1441004"/>
    <dbReference type="KEGG" id="tvo:TVG0471466"/>
    <dbReference type="eggNOG" id="arCOG03060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_2_4_2"/>
    <dbReference type="OrthoDB" id="9944at2157"/>
    <dbReference type="PhylomeDB" id="Q97BG8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10234">
        <property type="entry name" value="ASKHA_NBD_HSP70_DnaK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000001">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000071">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000078603" description="Chaperone protein DnaK">
        <location>
            <begin position="1"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="579"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="581"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="599"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="613" mass="66473" checksum="9A3F6F0586F78936" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-04-26" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BG9</accession>
    <name>DNAJ_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaJ</name>
        <name type="ordered locus">TV0486</name>
        <name type="ORF">TVG0470348</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaJ family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916745.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BG9"/>
    <dbReference type="SMR" id="Q97BG9"/>
    <dbReference type="STRING" id="273116.gene:9381268"/>
    <dbReference type="PaxDb" id="273116-14324701"/>
    <dbReference type="GeneID" id="1441003"/>
    <dbReference type="KEGG" id="tvo:TVG0470348"/>
    <dbReference type="eggNOG" id="arCOG02846">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_7_2"/>
    <dbReference type="OrthoDB" id="8967at2157"/>
    <dbReference type="PhylomeDB" id="Q97BG9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000034">
        <property type="entry name" value="Chaperone protein DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008035">
        <property type="entry name" value="PRK10767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010883">
        <property type="entry name" value="PRK14290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF48">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000070954" description="Chaperone protein DnaJ">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="152"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="168"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="194"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="208"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="1">
        <location>
            <begin position="139"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <sequence length="365" mass="41149" checksum="C401663ECFC51884" modified="2001-10-01" version="1">MAKDYYKILGVDRNASEEDIKKAFRELAKKWHPDLHPDNKAEAEEKFKEISEAYEVLSDPEKRRIYDQTGSVDFGGGGSNFNWDNFTHFSDINDIFNEIFGGNFGSDFFSGFGNRQSTRNIDLDMYTNLDISLEEAYYGTEKRIKFRRNAICPDCKGTGAKNGKLITCPTCHGTGQQRVVRGQGFFRMVTVTTCNTCGGKGRIPEEKCPRCNGTGTIVVDEDITVKIPRGATDNLRLRVSGKGQSYDGRTGDLYVILRIKQDKNLQRINDDLLLDQKINFGQAALGADIPIQIFNEKYNLKIPEGTQPGDVIKIKGAGMPHLNGHGSGDLLVRINVEVPKRLTAKQRELIRELFDIKENHKSWFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BL7</accession>
    <name>Q97BL7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S-methyl-5'-thioadenosine phosphorylase</fullName>
            <ecNumber evidence="5">2.4.2.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">5'-methylthioadenosine phosphorylase</fullName>
            <shortName evidence="5">MTA phosphorylase</shortName>
            <shortName evidence="5">MTAP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mtnP</name>
        <name evidence="7" type="ORF">TVG0426892</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homohexamer. Dimer of a homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.28" evidence="5"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916696.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BL7"/>
    <dbReference type="STRING" id="273116.gene:9381217"/>
    <dbReference type="PaxDb" id="273116-14324653"/>
    <dbReference type="GeneID" id="1440954"/>
    <dbReference type="KEGG" id="tvo:TVG0426892"/>
    <dbReference type="eggNOG" id="arCOG01327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_0_2_2"/>
    <dbReference type="OrthoDB" id="7681at2157"/>
    <dbReference type="PhylomeDB" id="Q97BL7"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00873"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000012">
        <property type="entry name" value="Probable 6-oxopurine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006334">
        <property type="entry name" value="PRK08564.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF3">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 5" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="50"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="204"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="5">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="5">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00063054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="257" mass="28911" checksum="3D2480F0682FE17B" modified="2001-10-01" version="1">MAYIGIIGGSGLYDLMAESKKVDVDTPYGKPSDLIEIGEVNGVEVAFLPRHGKKHTIPPHKVNYRANIWALHDLGVERIIGLNAVGSLREDYKPGDIVIPDQYIDFTKRRELTFYDGPQVYHISEADPFCPEMNELFYQTAKNLKIPVHNTGTYITIEGPRFSTRAESKMFRQFADIIGMTLVPEVGLAGELALCYSVIASITDYDVWSEKPVDAREVMTIMKQNDKKVRDILFNALPRIGKDRKCACSLRLDNAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BT4</accession>
    <name>Q97BT4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">TVG0361647</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916625.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BT4"/>
    <dbReference type="STRING" id="273116.gene:9381148"/>
    <dbReference type="PaxDb" id="273116-14324586"/>
    <dbReference type="GeneID" id="1440883"/>
    <dbReference type="KEGG" id="tvo:TVG0361647"/>
    <dbReference type="eggNOG" id="arCOG01721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038033_0_0_2"/>
    <dbReference type="OrthoDB" id="55795at2157"/>
    <dbReference type="PhylomeDB" id="Q97BT4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="entry name" value="Cytochrome Bc1 Complex, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005798">
        <property type="entry name" value="Cyt_b/b6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036150">
        <property type="entry name" value="Cyt_b/b6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005797">
        <property type="entry name" value="Cyt_b/b6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271:SF16">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00032">
        <property type="entry name" value="Cytochrom_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13631">
        <property type="entry name" value="Cytochrom_B_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81648">
        <property type="entry name" value="a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="Transmembrane di-heme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51003">
        <property type="entry name" value="CYTB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="409"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="480"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 N-terminal region profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
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        <location>
            <begin position="228"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAB59513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="537" mass="59960" checksum="85786B3237FF7186" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BT7</accession>
    <name>Q97BT7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0358554 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0358554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916622.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BT7"/>
    <dbReference type="STRING" id="273116.gene:9381145"/>
    <dbReference type="PaxDb" id="273116-14324583"/>
    <dbReference type="DNASU" id="1440880"/>
    <dbReference type="GeneID" id="1440880"/>
    <dbReference type="KEGG" id="tvo:TVG0358554"/>
    <dbReference type="eggNOG" id="arCOG02271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101251_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BT7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="149"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="209" mass="24019" checksum="77A8AD907D34A459" modified="2001-10-01" version="1">MRQNKNPVEALIEVERSDCWVTNLVLDRDKDAKIERLRIGELTTIHMVKPTMDRRALFNDLKKYPGIILKANLGGKDSIWVEGKSCASCRLLESFNIIVIGERSIGFDKVRYKIIAPSRNELNKAIRKLKEEDLNVIVLSISEYNKVELTPREKEIINAAFKLGYFDIDRQISMKDLAKNFGIKTASISDVMRRALKKIVMEYLEDSIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-29" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BW8</accession>
    <name>RNP1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 1</fullName>
            <shortName evidence="1">RNase P component 1</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Rpp29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp1</name>
        <name type="ordered locus">TV0337</name>
        <name type="ORF">TVG0337218</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916591.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BW8"/>
    <dbReference type="SMR" id="Q97BW8"/>
    <dbReference type="STRING" id="273116.gene:9381114"/>
    <dbReference type="PaxDb" id="273116-14324552"/>
    <dbReference type="GeneID" id="1440849"/>
    <dbReference type="KEGG" id="tvo:TVG0337218"/>
    <dbReference type="eggNOG" id="arCOG00784">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107020_2_1_2"/>
    <dbReference type="OrthoDB" id="39019at2157"/>
    <dbReference type="PhylomeDB" id="Q97BW8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF046110">
        <property type="entry name" value="RNaseP1Mthb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000128442" description="Ribonuclease P protein component 1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00754"/>
        </source>
    </evidence>
    <sequence length="89" mass="10217" checksum="6FB8590DDC1DCD5B" modified="2001-10-01" version="1">MIYISEFIGRHVEVIQSSNRYDVGISGQVSFETKNTFEITKGTGKVIVPKEGRIFTFDGKFKVDGSLINYRPEDRLREYRKILKKLGGN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BX4</accession>
    <name>RL2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl2</name>
        <name type="ordered locus">TV0331</name>
        <name type="ORF">TVG0334954</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916585.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BX4"/>
    <dbReference type="SMR" id="Q97BX4"/>
    <dbReference type="STRING" id="273116.gene:9381108"/>
    <dbReference type="PaxDb" id="273116-14324546"/>
    <dbReference type="GeneID" id="1440843"/>
    <dbReference type="KEGG" id="tvo:TVG0334954"/>
    <dbReference type="eggNOG" id="arCOG04067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_0_1_2"/>
    <dbReference type="OrthoDB" id="5987at2157"/>
    <dbReference type="PhylomeDB" id="Q97BX4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_uL2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_uL2_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007180">
        <property type="entry name" value="PRK09612.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000129730" description="Large ribosomal subunit protein uL2">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="234" mass="25007" checksum="CF44BC8BDF9DC1D1" modified="2001-10-01" version="1">MGKHIIPQRRGHGSLVYRSPSHRHVVDVKHLKPGEYVVKDIIHAPGRNSPLLELVDSTGKKVYQIAFTGAYVGQKIVAGNVDTINPGTTTVLANIPDGSYVYNIESSPGDGGEFCRSAGTFALVVSHGSRVTLKLPSGSMRDFDPRCRATVGVVAASGIIDAPILKAGTHVHYLRSKAKRPYTVRGVAMNAVNHPHGGGNHQHVGRPSTVGRNAPPGRKVGRLSPKRRKVNGSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BY3</accession>
    <name>SECG_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Preprotein translocase subunit SecG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein transport protein Sec61 subunit beta homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">secG</name>
        <name type="ordered locus">TV0322</name>
        <name type="ORF">TVG0327161</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in protein export. The function of the beta subunit is unknown, but it may be involved in stabilization of the trimeric complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. Can form oligomers of the heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59464" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59464.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916577.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BY3"/>
    <dbReference type="SMR" id="Q97BY3"/>
    <dbReference type="STRING" id="273116.gene:9381098"/>
    <dbReference type="PaxDb" id="273116-14324537"/>
    <dbReference type="GeneID" id="1440835"/>
    <dbReference type="KEGG" id="tvo:TVG0327161"/>
    <dbReference type="eggNOG" id="arCOG02957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208205_0_0_2"/>
    <dbReference type="OrthoDB" id="43651at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00751">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023531">
        <property type="entry name" value="Preprot_translocase_SecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002318">
        <property type="entry name" value="PRK01253.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000157282" description="Preprotein translocase subunit SecG">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="52"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="55" mass="6054" checksum="56EED5A0A0DE1C2A" modified="2004-02-16" version="2">MASDKKSEGFQSGAGLIRYFEEEEIKGPALDPKLVVYIGIAVAIMVELAKIFWPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BY8</accession>
    <name>Q97BY8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dimethyladenosine transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0324172</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916573.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BY8"/>
    <dbReference type="STRING" id="273116.gene:9381092"/>
    <dbReference type="PaxDb" id="273116-14324532"/>
    <dbReference type="GeneID" id="1440831"/>
    <dbReference type="KEGG" id="tvo:TVG0324172"/>
    <dbReference type="eggNOG" id="arCOG04131">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_0_1_2"/>
    <dbReference type="OrthoDB" id="9883at2157"/>
    <dbReference type="PhylomeDB" id="Q97BY8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="6">
        <location>
            <begin position="17"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="237" mass="27536" checksum="FD94BAAA709CC95E" modified="2001-10-01" version="1">MSYSKRLGQVFLRSRRIAEYEVDLLNGHPGDSVLEIGPGHGILTSILLDRGYYVTAVEKDRFVYNELLSIKNKNLNLFNMDFLDMPPQKYDFIIGNIPYYISSDVIFKLYDFEFSASVIMVQKEFADKLTNVKDSSRLYVNAYVRYEIDLKRYVGRKNFNPQPKVDSAILLLKKKKINLDIPLDYLDSKLKVMFSKKRKMISNIFEIYPESMGNRRPSDLTASEILDLVRLLHAHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BZ9</accession>
    <name>Q97BZ9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0315520</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916561.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BZ9"/>
    <dbReference type="STRING" id="273116.gene:9381081"/>
    <dbReference type="PaxDb" id="273116-14324521"/>
    <dbReference type="GeneID" id="1440819"/>
    <dbReference type="KEGG" id="tvo:TVG0315520"/>
    <dbReference type="eggNOG" id="arCOG02683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_14_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="PhylomeDB" id="Q97BZ9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="16"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="465" mass="50077" checksum="09324F50EFAFBC8E" modified="2001-10-01" version="1">MFSDFDSKLTRFQIKTVIVSGIGIFSDSYNLYAIALVYYIAAFYLNLTPPESALMTSGAFIGAAIGAILFGLIADKIGRKPVYGLDLALITLGSFLQFFAVNFTLLLIFRIILGLGIGGDYVLSPIITAENANSRDRGKLMIMTFPIMAAFGAMLAAFVDQVSTILLPSSLVWKIVLAFGGVPALFVIYFRRKITETARYSAIVKGSSVDIMSIEKETGTKIEITRDTKTYSYRLKTSLVLVLISSVLWILYDMYSSTFAIYGPITIAKNLGLTPISFTYYAEIFAGIPGAIVSAMLIDKIGRKRLITIGYLGVFFWLLLYGLLLMKSPIFGSKILSGILPTGLVGEAAFAGFSFYIMNYLFSAMGPASIIGGAMVTPEITPTKARGTSQAITVGLDRTADALGLTAFPLLLARLGLPFLILGFSAIAILSIIIMNVGIPEARGKSLEEITGETADTGIRGSIGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C13</accession>
    <name>RLME_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase E</fullName>
            <ecNumber evidence="1">2.1.1.166</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA Um2552 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmE</name>
        <name evidence="1" type="synonym">rrmJ</name>
        <name type="ordered locus">TV0292</name>
        <name type="ORF">TVG0303954</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(2552) in 23S rRNA + S-adenosyl-L-methionine = 2'-O-methyluridine(2552) in 23S rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42720"/>
            <dbReference type="Rhea" id="RHEA-COMP:10202"/>
            <dbReference type="Rhea" id="RHEA-COMP:10203"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.166"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.166" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916547.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="3DOU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.45 A"/>
        <property type="chains" value="A=17-196"/>
    </dbReference>
    <dbReference type="PDBsum" id="3DOU"/>
    <dbReference type="AlphaFoldDB" id="Q97C13"/>
    <dbReference type="SMR" id="Q97C13"/>
    <dbReference type="STRING" id="273116.gene:9381066"/>
    <dbReference type="PaxDb" id="273116-14324507"/>
    <dbReference type="DNASU" id="1440805"/>
    <dbReference type="GeneID" id="1440805"/>
    <dbReference type="KEGG" id="tvo:TVG0303954"/>
    <dbReference type="eggNOG" id="arCOG00079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_4_4_2"/>
    <dbReference type="OrthoDB" id="26307at2157"/>
    <dbReference type="PhylomeDB" id="Q97C13"/>
    <dbReference type="EvolutionaryTrace" id="Q97C13"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF13">
        <property type="entry name" value="PRE-RRNA 2'-O-RIBOSE RNA METHYLTRANSFERASE FTSJ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155574" description="Ribosomal RNA large subunit methyltransferase E">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
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    </feature>
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        <location>
            <position position="111"/>
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    <feature type="strand" evidence="2">
        <location>
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    <feature type="strand" evidence="2">
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    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01547"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3DOU"/>
        </source>
    </evidence>
    <sequence length="197" mass="22604" checksum="DC1682A2E42D05E5" modified="2001-10-01" version="1">MTGDRRDEYYWKAKKEQLRSRAAFKLEFLLDRYRVVRKGDAVIEIGSSPGGWTQVLNSLARKIISIDLQEMEEIAGVRFIRCDIFKETIFDDIDRALREEGIEKVDDVVSDAMAKVSGIPSRDHAVSYQIGQRVMEIAVRYLRNGGNVLLKQFQGDMTNDFIAIWRKNFSSYKISKPPASRGSSSEIYIMFFGFKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C51</accession>
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            <fullName evidence="1">TVG0266297 protein</fullName>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
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            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Koike H."/>
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                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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                <person name="Aramaki H."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="44218" checksum="9E67045D6A7F3797" modified="2001-10-01" version="1">MPVVHPTVHRKPRILSNFSDLSLYYISYNLFAFSNGLFGTFINIFFFSKFNLISVVEYQAAIQGTQTLMFVFSGYLIRILNAKYVYSIGNYARSLIIFALILAKGPLFNPIIFGLIYGIPSGIFWSGNNTLSQAVTRSKDRFGFLSTNSTIAGAAGLVAPLLAGFIISISYGRGLERYSTDFLISALLLIIAGTVAIAIREKGEGRWKYRARNTIIGRDEKYSKFKTYFFLSNILTITVSTIVPLYIFSLTKSYVLIGFFGTFLSLVNLISNATVVSLRRIVPNISQISIVVVMLSSLLYFIHVWNNVASAYVASALIIYFMVPLNNSAMSNFMGFLDGVQRTAVYWINREYYLVSGRIFSFAVLAVLSSLGFPIGLLMFSLPVFSLSVLGFIKVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
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    <name>Q97C82_THEVO</name>
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            <taxon>Thermoplasmatota</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916476.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C82"/>
    <dbReference type="STRING" id="273116.gene:9380992"/>
    <dbReference type="PaxDb" id="273116-14324435"/>
    <dbReference type="GeneID" id="1440736"/>
    <dbReference type="KEGG" id="tvo:TVG0229397"/>
    <dbReference type="eggNOG" id="arCOG05402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582969_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="188" mass="21944" checksum="D2CC2921CEAB2704" modified="2001-10-01" version="1">MYFVATIGKLHGKIAGYDYRTLYRYVMSLNPTKIYVTYLTHSDYDSGNYGEEIKLIMSDESLSERVSFCGADESKYKKFYEDYVNINKSSPEFVVKLNVLDIINTTVNSYLESYWKSAETVNSEVTDSLFRAKHRLMTAMFPEMELETWEKKHKEILENIDMTGVTPNTMILCPAESRYWFIDHFGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CA3</accession>
    <name>Q97CA3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase BadF/BadG/BcrA/BcrD type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0204668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760294.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CA3"/>
    <dbReference type="STRING" id="273116.gene:9380969"/>
    <dbReference type="PaxDb" id="273116-14324413"/>
    <dbReference type="GeneID" id="1441685"/>
    <dbReference type="KEGG" id="tvo:TVG0204668"/>
    <dbReference type="eggNOG" id="arCOG03659">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016274_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CA3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052519">
        <property type="entry name" value="Euk-type_GlcNAc_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43190">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43190:SF3">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase BadF/BadG/BcrA/BcrD type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="333" mass="36370" checksum="83D0D42FCD75FC7F" modified="2001-10-01" version="1">MILGVDGGSTKTVAVVYDDESGRIAGVGISGPANYTNSPREEAMKNIKNSVYEAVDDADVNIEDIGFKVFGLAGIGDSKEATAEGEEIVRSIAGEAIVVNDGYAAYKFANLNENGLVFAPGTGSVGFTMIDGKLSRFGGWGWFIGDEASASWMAKEALIYAEREKDGIIEAGIADKAETYFNLDLYEVVYKIMKGTIPKRTVAAFSPIISELAVEGNKYAISIFEEASNYIADLINAKSGIFSGKAIFSVLGGTMLAGRFYWDMIKKKTMVPVNIHFGYQVVIGDIIIGLEKKRTVDFNERDKLIRMLNDKISEKRDKMKEFLFMDKIPDSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CB2</accession>
    <name>Q97CB2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">threonine ammonia-lyase</fullName>
            <ecNumber evidence="4">4.3.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0197397</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916446.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CB2"/>
    <dbReference type="STRING" id="273116.gene:9380960"/>
    <dbReference type="PaxDb" id="273116-14324404"/>
    <dbReference type="GeneID" id="1441676"/>
    <dbReference type="KEGG" id="tvo:TVG0197397"/>
    <dbReference type="eggNOG" id="arCOG01431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_1_2"/>
    <dbReference type="OrthoDB" id="9915at2157"/>
    <dbReference type="PhylomeDB" id="Q97CB2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00054"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000007">
        <property type="entry name" value="L-threonine dehydratase catabolic TdcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000005">
        <property type="entry name" value="Threonine dehydratase catabolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005789">
        <property type="entry name" value="Thr_deHydtase_catblc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01127">
        <property type="entry name" value="ilvA_1Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="8">
        <location>
            <begin position="332"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="406" mass="44484" checksum="D4B3F75B1B98D379" modified="2001-10-01" version="1">MENLEIPSFDEIIEAQRYLEGKVNRTPLIRSTTIGKEYGADIYFKLENFQKTGSFKSRGAIFRFSKLSEDEKRHGVITASAGNHAQGVAYAAMINGIDAKIVMPEYTIPQKVNAVISYGAHVILKGSDYDEAHRYADEIAKQEGRIFIEAFNDRWVISGQGTIGLEIMEDLPDVDIILVPVGGGGLISGIALAAKHASNKVKVIGIESELSDSMKASLREGKIVAHTSGVSICDGISVKYPGVLTFDIARKYVDDIVTVTEEYVSKAIYKLFERNKIVAEPSGAVGLAAIMEGKVDVKGKKVAIVVSGGNINPLLMSKIIYKELENLGQLVRIECTIPDRPGNLYRIAMAIAENGGNIYHAEVDNLRKETPPGFQSVTFTVNVRGQDHLDRIIGSLREMGYLFRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CE9</accession>
    <name>Q97CE9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TVG0161955 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0161955</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916407.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CE9"/>
    <dbReference type="STRING" id="273116.gene:9380922"/>
    <dbReference type="PaxDb" id="273116-14324366"/>
    <dbReference type="GeneID" id="1441637"/>
    <dbReference type="KEGG" id="tvo:TVG0161955"/>
    <dbReference type="eggNOG" id="arCOG03654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_634047_0_0_2"/>
    <dbReference type="OrthoDB" id="57314at2157"/>
    <dbReference type="PhylomeDB" id="Q97CE9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="432" mass="46444" checksum="40D0C6FB1C84D286" modified="2001-10-01" version="1">MGSRDLGVGSSIMQSIGGSGPMLNIVGVFAIIASFSSINLVYIVIGAFIISLVNIYMPYRASKIIRSNGGYYTLNGIFMGKRTGLFVSILYLIYGFAAFPSITIFTVSILYYLSGSLTMSVVAADAIAASIAFVTMGGTYVSAKVLKILGALEIMFIVLLDVSMISHGSGVISPYALSSATTPNFWYGIMFGLLMFSGLGSSLFISENTKNSKVSVSRGIAIAYAVSGSLMVVTALAIERFLGNNISYYSSNPFYIIGSVKSVLGSYFYIFFLFFSVSSAFNLAVAYLNAFSNSAVKMGEDGIIAPLSRRKVLAISLALDIVASSVFPILFGPFVAFVTITGLVSASYLTVHLISNVSLYKHISASREVIGKIMLIASSFTVLVTLAYSILADIYTSMLVDIFFAIALLVAIYSVFYILRSSRYTSIIFHYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CF4</accession>
    <name>Q97CF4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Proline permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0152244</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916402.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CF4"/>
    <dbReference type="STRING" id="273116.gene:9380917"/>
    <dbReference type="PaxDb" id="273116-14324361"/>
    <dbReference type="GeneID" id="1441632"/>
    <dbReference type="KEGG" id="tvo:TVG0152244"/>
    <dbReference type="eggNOG" id="arCOG01316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_15_0_2"/>
    <dbReference type="OrthoDB" id="19182at2157"/>
    <dbReference type="PhylomeDB" id="Q97CF4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10322">
        <property type="entry name" value="SLC5sbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050277">
        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF8">
        <property type="entry name" value="MONOCARBOXYLIC ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086">
        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="482" mass="52610" checksum="C836F79F3907D964" modified="2001-10-01" version="1">MSVGVEVLTISFVVIVIAAVIGFLARSKNVSLEQWAVGGRNIGALLIWFLLAGEIYTAFTFLGVPGYAYAFGAPAYYALSYGVLAYLTSYFILPKIWRISKKKNIMTQPDFFKERYQSTLLATIVAVVGVVFMVPYLELQVSAITDILEVMGYNVIMPAYVMIITFVLTAGYVIMNGLRSATFTAFFKDGLALIVIFSALIVIPAVQFGDLNNMMHRMVTNYSEYLYFPGHSELGLSWFVSVVLMSALGFFMWPHSFQSYISAKNDNVLKKNAVFLPVYNVLLVITVFMGFAALLYNPHLSNADDAFLIVARASFPSWWNGVVAGAITLGSMVPASVLLIASSTIISKNLYKDLINKKASDSSVKFIAQVFVAIITGVALLFAIEFPALFVNLLLLGYSGITLFFPGIVMGITWRKVTAVGVISGIITGLIVNAVFFLPLSSYSHIIYPGLVGLFADTVVTILVSLVTKAPSGEVIEEFVTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CG9</accession>
    <name>HMGCS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="1">HMG-CoA synthase</shortName>
            <shortName evidence="1">HMGCS</shortName>
            <ecNumber evidence="1">2.3.3.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0132</name>
        <name type="ORF">TVG0141026</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds that are building blocks of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:10189"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with acetoacetyl-CoA thiolase that catalyzes the precedent step in the pathway and with a DUF35 protein. The acetoacetyl-CoA thiolase/HMG-CoA synthase complex channels the intermediate via a fused CoA-binding site, which allows for efficient coupling of the endergonic thiolase reaction with the exergonic HMGCS reaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Archaeal HMG-CoA synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916386.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CG9"/>
    <dbReference type="SMR" id="Q97CG9"/>
    <dbReference type="STRING" id="273116.gene:9380901"/>
    <dbReference type="PaxDb" id="273116-14324346"/>
    <dbReference type="GeneID" id="1441616"/>
    <dbReference type="KEGG" id="tvo:TVG0141026"/>
    <dbReference type="eggNOG" id="arCOG01767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_7_0_2"/>
    <dbReference type="OrthoDB" id="5812at2157"/>
    <dbReference type="PhylomeDB" id="Q97CG9"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00102"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01409">
        <property type="entry name" value="HMG_CoA_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004656">
        <property type="entry name" value="HMG_CoA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00748">
        <property type="entry name" value="HMG_CoA_syn_Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003274">
        <property type="entry name" value="PRK04262.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000057628" description="Hydroxymethylglutaryl-CoA synthase">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="1">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <note>ligand shared with acetoacetyl-CoA thiolase</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <note>ligand shared with acetoacetyl-CoA thiolase</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01409"/>
        </source>
    </evidence>
    <sequence length="351" mass="38248" checksum="C68527D7655A44D6" modified="2001-10-01" version="1">MSGIVTYGSYIPRYRIKPDEIARVWGENPERIKNGIFILSKSVPAPDEDVATISVEAARNALKRKKINPKEIGAIYVGSESHPYAVKPTATIVGSAIGVDFSLFAADYEFACKAGTAGMQNVKAMVDAGMIKYGLAIGADTSQGAPGDALEYSASAGGSAFIIGKDDVIAEINSTLSVASDTPDFWRREGQPYPSHGERFTGEPAYFRHVVNAAKMMMERMETQPKDYDYVVFHQPNGKFPTRAAKLLGFEEKQYKDGLITPYIGNTYSGSMMTGLSSILDVSKPGDHILAVSFGSGAGSDAFDITVTDRIEEMDRNKAPTIKKMLEKIKWVDYAIYAKYKKKIVLGDGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CI0</accession>
    <name>Q97CI0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endoribonuclease Nob1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0128172</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Toxic component of a type II toxin-antitoxin (TA) system. Processes pre-16S-rRNA at its 3' end (the D-site) to yield the mature 3' end.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916376.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CI0"/>
    <dbReference type="SMR" id="Q97CI0"/>
    <dbReference type="STRING" id="273116.gene:9380889"/>
    <dbReference type="PaxDb" id="273116-14324335"/>
    <dbReference type="DNASU" id="1441606"/>
    <dbReference type="GeneID" id="1441606"/>
    <dbReference type="KEGG" id="tvo:TVG0128172"/>
    <dbReference type="eggNOG" id="arCOG00721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109674_1_0_2"/>
    <dbReference type="OrthoDB" id="27944at2157"/>
    <dbReference type="PhylomeDB" id="Q97CI0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000020">
        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009148">
        <property type="entry name" value="PRK12496.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PIN" evidence="8">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="163" mass="18333" checksum="5FC4839FD1583FD7" modified="2001-10-01" version="1">MKYVLDTSAIISRNINLLDGDLLFPSSVIGEIRKGKLKYMLDALFPMIRIVDPDQYYVRSVEDAAHKTGDLMNLSGTDIEVIAVAMQYGATIVTDDYSIQNVASALKIAFMNANIKSISKQISWVYRCTGCKKIFHEPVKVCDICGHNVKRHYDKKRSAVKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CI7</accession>
    <name>Q97CI7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0121023 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0121023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CI7"/>
    <dbReference type="PaxDb" id="273116-14324328"/>
    <dbReference type="KEGG" id="tvo:TVG0121023"/>
    <dbReference type="HOGENOM" id="CLU_2713068_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="72" mass="8199" checksum="39A25F9C16C8A3DB" modified="2001-10-01" version="1">MLQPLQHQLQHRKMPKIEGLLTNAGFFMVTCCNMMQHQLQHKKLRIIEGFLTSGHLHGDHVATVATDHVATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CJ0</accession>
    <name>Q97CJ0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cyclic oligoadenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">TVG0115828</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Cas10/Csm1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916368.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CJ0"/>
    <dbReference type="STRING" id="273116.gene:9380879"/>
    <dbReference type="PaxDb" id="273116-14324325"/>
    <dbReference type="GeneID" id="1441598"/>
    <dbReference type="KEGG" id="tvo:TVG0115828"/>
    <dbReference type="eggNOG" id="arCOG02666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017487_0_0_2"/>
    <dbReference type="OrthoDB" id="57429at2157"/>
    <dbReference type="PhylomeDB" id="Q97CJ0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09680">
        <property type="entry name" value="Cas10_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054767">
        <property type="entry name" value="Cas10-Cmr2_palm2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013408">
        <property type="entry name" value="Cas10/Csm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052117">
        <property type="entry name" value="Cas10/Csm1_subtype-III-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041062">
        <property type="entry name" value="Csm1_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02578">
        <property type="entry name" value="cas_TM1811_Csm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36528">
        <property type="entry name" value="CRISPR SYSTEM SINGLE-STRAND-SPECIFIC DEOXYRIBONUCLEASE CAS10/CSM1 (SUBTYPE III-A)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36528:SF1">
        <property type="entry name" value="CRISPR SYSTEM SINGLE-STRAND-SPECIFIC DEOXYRIBONUCLEASE CAS10_CSM1 (SUBTYPE III-A)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22335">
        <property type="entry name" value="Cas10-Cmr2_palm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18211">
        <property type="entry name" value="Csm1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HD" evidence="14">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="13">
        <location>
            <begin position="518"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAB59253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="775" mass="90617" checksum="7294D46EF2AAE216" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CM1</accession>
    <name>Q97CM1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mannose-1-phosphate guanyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0079558</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916337.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CM1"/>
    <dbReference type="STRING" id="273116.gene:9380846"/>
    <dbReference type="PaxDb" id="273116-14324294"/>
    <dbReference type="GeneID" id="1441567"/>
    <dbReference type="KEGG" id="tvo:TVG0079558"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_2_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="PhylomeDB" id="Q97CM1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056818">
        <property type="entry name" value="GlmU/GlgC-like_hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24894">
        <property type="entry name" value="Hexapep_GlmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-1-phosphate adenylyltransferase/Bifunctional protein GlmU-like C-terminal hexapeptide" evidence="2">
        <location>
            <begin position="293"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="359" mass="39024" checksum="DC26013DC34A1D66" modified="2001-10-01" version="1">MTVKGVLMAGGKGTRLRPITYSIPKPLVPVAGKPVISYILDSFYNAGVKDIIITTGYKFEALIKGVLENKFSDQNILFSVEKDPAGTAGGVKLAENFIDDTFVVGSGDILIDFDVSKMIEEHKKRGANITIALTRVDDPSQFGIAEVDDEGYVKRFLEKPKKSETFSNTINAGVYVIEPSVLEYIPKGVQFDFAKDLFPKAMANGIKIYTYEINGVWLDAGRPGDLIKANQIMVDKYGDRNINGSRMILKARIPDGVNVSGPTYIGEGVAIGKGSSIDSSTIYEGVQIGNDVEIKNSVIMSSCRILDGSKISDSVIMQNTVIGEACEIRNSVLSQKLNLQKGSRVFDVALSSEIIQDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CN3</accession>
    <name>Q97CN3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">propanoyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.176</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Propanoyl-CoA C-acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0067506</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.176" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916325.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CN3"/>
    <dbReference type="STRING" id="273116.gene:9380834"/>
    <dbReference type="PaxDb" id="273116-14324282"/>
    <dbReference type="GeneID" id="1441555"/>
    <dbReference type="KEGG" id="tvo:TVG0067506"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="OrthoDB" id="56281at2157"/>
    <dbReference type="PhylomeDB" id="Q97CN3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004720">
        <property type="entry name" value="PRK06064.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="8">
        <location>
            <begin position="9"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="9">
        <location>
            <begin position="242"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="390" mass="41820" checksum="62C832A60A38BAD2" modified="2001-10-01" version="1">MVRVGIIGYGISKFGKRNDASMYDLIWEAGSEALKTSGVDKKDVEYLVVSNMGMWSSEELPAVVAGEVLGTERAGTNRVEAACASGSSAISSAYDAIASGHVRMAMALGVEKMSEVDTGTAIELIGRAGNFLWEYEFFGLTFPGYYALHATAYLDKYGGKEEDLALVSVKNHHYAHFNPKAQFTNDISVEDVMSSRYIASPLKLYDSSPITDGAAAVVLASEDVAKQYTDTPVWIDAIGSNLGTSNLSKRPDFTGIEAAREAARKAYKVAGIDSEKPYNFIDGADVHDCFSIAELMAYEDLGFAGKGEALELIREGQTYIGGRIPVNVDGGLISKGHPIGATGVAMAVEATKQLLRKAENGRQIDVNKGRFLTHNVGGTGHYAFVTVYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CQ4</accession>
    <name>Q97CQ4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">H+-transporting ATP synthase subunit K</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0049983</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010900427.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CQ4"/>
    <dbReference type="STRING" id="273116.gene:9380812"/>
    <dbReference type="PaxDb" id="273116-14324261"/>
    <dbReference type="GeneID" id="1441534"/>
    <dbReference type="KEGG" id="tvo:TVG0049983"/>
    <dbReference type="eggNOG" id="arCOG02455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148047_3_3_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18181">
        <property type="entry name" value="ATP-synt_Vo_Ao_c_TtATPase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="7">
        <location>
            <begin position="11"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="75" mass="7676" checksum="0499A9FAB9EDEA2F" modified="2001-10-01" version="1">MVTTVDALLAIAASISIAGGLIGTGMAQQGIGAAGMGIIAEKPEKFGQVLFFFVIPETLWVIGLALGIILLLHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CQ8</accession>
    <name>Q97CQ8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0046971</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CQ8"/>
    <dbReference type="STRING" id="273116.gene:9380808"/>
    <dbReference type="PaxDb" id="273116-14324257"/>
    <dbReference type="KEGG" id="tvo:TVG0046971"/>
    <dbReference type="eggNOG" id="arCOG03032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003728_11_3_2"/>
    <dbReference type="PhylomeDB" id="Q97CQ8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="54"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="88"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="122"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="240" mass="27990" checksum="99A2591E5C741430" modified="2001-10-01" version="1">MKKGIFMVDEFKLNNKEQLADDYNERGISYFNIGKYDKAVEEFTKAISIINDDADLYHNRGMAYYSMKAYDQAIEDFERSISLDPNSSDYHNALGSVYEDMGNYEKALEEFNSAIRLEDDLPDYYYNRGNVYWKLGEIEKAIQDYSKAADLDYTDQIYVYKKYEALTSLGRYDEALETVDKAIKVVPANYNYLAMKAELLIKMKRYEEARPVIEEVEKLNPGNPDAARLMAQINMKRQNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CR8</accession>
    <name>PYRG_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTP synthase</fullName>
            <ecNumber evidence="1">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="1">CTP synthetase</shortName>
            <shortName evidence="1">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrG</name>
        <name type="ordered locus">TV0033</name>
        <name type="ORF">TVG0036820</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UTP + NH4(+) + ATP = CTP + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CR8"/>
    <dbReference type="SMR" id="Q97CR8"/>
    <dbReference type="STRING" id="273116.gene:9380798"/>
    <dbReference type="PaxDb" id="273116-14324247"/>
    <dbReference type="KEGG" id="tvo:TVG0036820"/>
    <dbReference type="eggNOG" id="arCOG00063">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_2"/>
    <dbReference type="OrthoDB" id="52769at2157"/>
    <dbReference type="PhylomeDB" id="Q97CR8"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000009">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000002">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138273" description="CTP synthase">
        <location>
            <begin position="1"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="294"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="1">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="502"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="147"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="185"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="185"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="375"/>
            <end position="378"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <sequence length="540" mass="60572" checksum="4E0C7B2382FF0E42" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CS9</accession>
    <name>Q97CS9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0021463</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916279.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CS9"/>
    <dbReference type="STRING" id="273116.gene:9380787"/>
    <dbReference type="PaxDb" id="273116-14324236"/>
    <dbReference type="GeneID" id="1441508"/>
    <dbReference type="KEGG" id="tvo:TVG0021463"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="PhylomeDB" id="Q97CS9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051782">
        <property type="entry name" value="ABC_Transporter_VariousFunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939:SF1">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="259" mass="28395" checksum="5E1537A7BE8F5AF7" modified="2001-10-01" version="1">MNCAIEIEDLYMYYGEKPALKGVNLTIPSGSIYGILGPNGSGKTTLMKILVSLLSKKSGRVSVCGLDVDKDQMKIKEIAGYIPETPVLYESMTPLELFDFVGSVRHIPVDSYARRLNALIDAFSIKDYLNEFIGSLSFGNKQKVAIISALVHDPKVLIVDEGMNGLDPRSARIFKDILMDMKNRGKIVVFSTHILEIAENLCDSVAILYEGSIVANGTIRDLESSAPENSQNLEDIFLKLTNSEDLGDVVKSLRGEVGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-11-16" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CU2</accession>
    <name>RL39_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL39</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L39e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl39e</name>
        <name type="ordered locus">TV0009</name>
        <name type="ORF">TVG0008653</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916266.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CU2"/>
    <dbReference type="SMR" id="Q97CU2"/>
    <dbReference type="STRING" id="273116.gene:9380774"/>
    <dbReference type="PaxDb" id="273116-14324223"/>
    <dbReference type="GeneID" id="1441494"/>
    <dbReference type="KEGG" id="tvo:TVG0008653"/>
    <dbReference type="eggNOG" id="arCOG04177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181948_4_0_2"/>
    <dbReference type="OrthoDB" id="65887at2157"/>
    <dbReference type="PhylomeDB" id="Q97CU2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein-like L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_eL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020083">
        <property type="entry name" value="Ribosomal_eL39_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002316">
        <property type="entry name" value="PRK01242.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00051">
        <property type="entry name" value="RIBOSOMAL_L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000127065" description="Large ribosomal subunit protein eL39">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="51" mass="6288" checksum="3FD1AE4659F905F5" modified="2001-10-01" version="1">MSRNKELGRKIRLMKKVKQNRRVPGWVMMRTARKVTQNPLRRNWRRGNLKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-14" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>P58227</accession>
    <name>RF1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Peptide chain release factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Translation termination factor aRF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">prf1</name>
        <name type="ordered locus">TV0588</name>
        <name type="ORF">TVG0577437</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58227"/>
    <dbReference type="SMR" id="P58227"/>
    <dbReference type="STRING" id="273116.gene:9381376"/>
    <dbReference type="PaxDb" id="273116-14324803"/>
    <dbReference type="KEGG" id="tvo:TVG0577437"/>
    <dbReference type="eggNOG" id="arCOG01742">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035759_3_0_2"/>
    <dbReference type="PhylomeDB" id="P58227"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00424">
        <property type="entry name" value="Rel_fact_arch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020918">
        <property type="entry name" value="Peptide_chain-rel_aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000143187" description="Peptide chain release factor subunit 1">
        <location>
            <begin position="1"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="418" mass="47951" checksum="3B72B219563638FA" modified="2001-08-14" version="1">MYFMADDEQIRRYEFKRALEELSKLHGRGTELISLYIPPEKQISDVVAYLRDEYSTSSNIKSKSTRKNVLAAIESIMSRLKYYRFPPPNGLVFFVGHIATRGDQTEMYTKIIEPPEPITTFMYKCDSEFHTEMLKAMLDEKEIYGLIVIDRKEATVGFLNGTRIEVVENVQSQVPSKHHQGGQSSRRFERLIEIAANEFFKKVGEIANNAFIPRIKEIRAIFLGGPGATKEYFFEKDYLRNEVKDKIKDLFDVGYTDESGLRELVEKASESIKDMKISREKDLMDRFLREVKKPDGGLAVYGEKAIRDALEQKMVDLLLISEGIRRVKYTYKCPTCGEEKTFSEEPSEWPVCDKDGTPLELIGEDDMIEDLYKLAKESGAQVEIISDQSDEGKLLKQAFGGLAAVLRFIRKDNVQNVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q978A3</accession>
    <name>Q978A3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1566346 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1566346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917743.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978A3"/>
    <dbReference type="STRING" id="273116.gene:9382328"/>
    <dbReference type="PaxDb" id="273116-14325753"/>
    <dbReference type="GeneID" id="1442203"/>
    <dbReference type="KEGG" id="tvo:TVG1566346"/>
    <dbReference type="eggNOG" id="arCOG12034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529310_0_0_2"/>
    <dbReference type="OrthoDB" id="55589at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="175" mass="19866" checksum="62945E141DA17232" modified="2001-10-01" version="1">MRYRLAYVSIGIAIMIVDLVVSLKYPKILIYSYLGSFLGFFVPFGIGLSLLLYGAFRARDYGVNPVIGILGSGGFTRVVRQNRIQGTRMWKTDHDILSDLPGAVKSPDGKTVEYSNLFIRKPYGTTKHTRKLNEAANKYIAGELDEEGFYNYIGKHGSKEEQMLDFIKKFKNDYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q978C9</accession>
    <name>Q978C9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1538342 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1538342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917717.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978C9"/>
    <dbReference type="STRING" id="273116.gene:9382302"/>
    <dbReference type="PaxDb" id="273116-14325727"/>
    <dbReference type="GeneID" id="1442177"/>
    <dbReference type="KEGG" id="tvo:TVG1538342"/>
    <dbReference type="HOGENOM" id="CLU_2103652_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="115" mass="13145" checksum="156F8C11AE678126" modified="2001-10-01" version="1">MAKVSLIGYSIRVRKTRSKEYADLYSFDGTNDLFSVFSQYFSKYSNSSSNAIYPVEDVPETDNKTMSIDPVQINGRLIQGLIRIGDYGYITPIVNRKSRKLSYTRNIEDTELVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q978E2</accession>
    <name>Q978E2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fuculose-1-phosphate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1526682</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978E2"/>
    <dbReference type="STRING" id="273116.gene:9382289"/>
    <dbReference type="PaxDb" id="273116-14325714"/>
    <dbReference type="KEGG" id="tvo:TVG1526682"/>
    <dbReference type="eggNOG" id="arCOG04226">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_3_0_2"/>
    <dbReference type="PhylomeDB" id="Q978E2"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050197">
        <property type="entry name" value="Aldolase_class_II_sugar_metab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22789:SF0">
        <property type="entry name" value="3-OXO-TETRONATE 4-PHOSPHATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22789">
        <property type="entry name" value="FUCULOSE PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="221" mass="24373" checksum="9AEF29A53430A783" modified="2001-10-01" version="1">MICMYEKEKEQVVEACRRIAQDGLTVGSWGNISVRADDGKIAITPSGKNYNKTEKEDIVITSIDGDILEGHLTPSSERLMHYEIYRKRSDVKAIVHTHSVYSSILSVVDDDLPAITEDVIMILGRSVRVAKYAMTGTVDLAKNVVEALGDSNATIMKNHGAVAVGKDIDRAMAAAYVLEKSAKIYVMAKLLGKVSAVPDDDVDKLIGISEAYLKQWDRWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q978K4</accession>
    <name>Q978K4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1458422 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1458422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917643.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978K4"/>
    <dbReference type="STRING" id="273116.gene:9382220"/>
    <dbReference type="PaxDb" id="273116-14325650"/>
    <dbReference type="GeneID" id="1442101"/>
    <dbReference type="KEGG" id="tvo:TVG1458422"/>
    <dbReference type="eggNOG" id="arCOG07362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811538_0_0_2"/>
    <dbReference type="OrthoDB" id="376022at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="143" mass="14812" checksum="00C2209387EF50A7" modified="2001-10-01" version="1">MPFTVKNGSIAAGSVAGILVMIAIATATPLSWLASGFVAGIAAKGMGRGASSSLVSSLVTLFIMVTLAIFILPSGLSQLHSTIGNSFLYNFIAYSLNTMSIPTNKLIKEIILDDVLLSIIGGLIGGSIFKSVLPREDVPQAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q978L3</accession>
    <name>Q978L3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1447366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917634.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978L3"/>
    <dbReference type="STRING" id="273116.gene:9382211"/>
    <dbReference type="PaxDb" id="273116-14325641"/>
    <dbReference type="GeneID" id="1442092"/>
    <dbReference type="KEGG" id="tvo:TVG1447366"/>
    <dbReference type="eggNOG" id="arCOG00655">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_1113925_0_0_2"/>
    <dbReference type="OrthoDB" id="57195at2157"/>
    <dbReference type="PhylomeDB" id="Q978L3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Menaquinone_biosynth"/>
    </dbReference>
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        <property type="entry name" value="MqnA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37690">
        <property type="entry name" value="CHORISMATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37690:SF1">
        <property type="entry name" value="CHORISMATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02621">
        <property type="entry name" value="VitK2_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="249" mass="28220" checksum="B2203A07A41FF1B8" modified="2001-10-01" version="1">MISIINFSHSDPLYLGFSNKEHVLRNNPFENLKLVLDGKVDASMVSLLSYLENSNDLILLKTATIHSTRKTISTILISRKPGMDRKIRVAITKNTRTTEFYLSLVLERLGIDYTFVRTDKTEAEDLLAIEDYALLNGDEALRVYSKKYPLIADVGNMFSKLYGMEPVYAVTVSREPFDYSAIDEAILSSYSYKDECSMALSSRLGIPLSVASDYYSVIRYEYGIEVERTINFVRSLITASNGKARIRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q978L6</accession>
    <name>Q978L6_THEVO</name>
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        <recommendedName>
            <fullName evidence="6">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1444614</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="273116.gene:9382208"/>
    <dbReference type="PaxDb" id="273116-14325638"/>
    <dbReference type="KEGG" id="tvo:TVG1444614"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1017890_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978L6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="AA-Vitamin_Transporters"/>
    </dbReference>
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        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322:SF2">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="20"/>
            <end position="41"/>
        </location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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    </feature>
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        <location>
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            <end position="219"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="2"/>
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        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="137"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="273" mass="30244" checksum="28A8A8D58F03E1CF" modified="2001-10-01" version="1">MAFFWALNYVMVKFAYAYDLSSSILLMRVLYASIFSSLIFIRQIKLPKSPLEHLKVFILANLNITGFFSLWFFGETGVSAAVTSIVIYSYPILATAFSYLFLGDRFGAMKTLGLILGFAGLVIIFIRSINVENLVDLIMLLVAAISWAIGTVFYRKYLLGYDSAGLNTLQLLYALPVVLLISLFTGGINIGLLQLKFNLIMIYMGSFGTAIAYFIYLYLYRKYKVSSISAYFFLVPAISVVLAAVLLKEPISALTIAGFAVMSAGIFLSGSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q978M5</accession>
    <name>Q978M5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1435680</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978M5"/>
    <dbReference type="STRING" id="273116.gene:9382198"/>
    <dbReference type="PaxDb" id="273116-14325629"/>
    <dbReference type="KEGG" id="tvo:TVG1435680"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_732846_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978M5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050189">
        <property type="entry name" value="MFS_Efflux_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124:SF3">
        <property type="entry name" value="CHLORAMPHENICOL EFFLUX PUMP RV0191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124">
        <property type="entry name" value="PURINE EFFLUX PUMP PBUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="364" mass="40052" checksum="D1C5764EA5A01207" modified="2001-10-01" version="1">MRFFIPAILPFIISALKISVYQASLLITAYWIGYTAFQIPAGIISDRFGTAKTSKASFLGLTIAFFLMPIAMHEYLLIFLLQLLLGSLSSIVYVSGISLVQRSADQSNRSLFIGIFQIGFFMGSSVGEYIVLELLDISFYYSYIAVSLLLLISLTLDFLFQNEPGLSKKNNEIIPRGILYVSLIRFSAGFMYLGFLSLFTTFLVYDGIAPLSKAYAYAWIPAIGGIAGSPLGGMISRKMWHAKALMAIVPIIVFSVVLMLIGIVNRNYVPPLSFATGFLYGLYAGPSMGLASEVGREENLGTSSGILNFSSQVGGIISPLAIGYLFTYFGNFKIPFAVISVLSFVIILFPLAIFLRRLFRHTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q978N7</accession>
    <name>Q978N7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1425262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917612.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978N7"/>
    <dbReference type="STRING" id="273116.gene:9382186"/>
    <dbReference type="PaxDb" id="273116-14325617"/>
    <dbReference type="GeneID" id="1442070"/>
    <dbReference type="KEGG" id="tvo:TVG1425262"/>
    <dbReference type="eggNOG" id="arCOG01668">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138323_0_0_2"/>
    <dbReference type="OrthoDB" id="41780at2157"/>
    <dbReference type="PhylomeDB" id="Q978N7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="114" mass="12868" checksum="9C86A2C1716D878D" modified="2001-10-01" version="1">MRNLAVIISSGKNEKEKVLTALTFANVAKKNKLFDDVKLILFGPSERLVAENDKDVMKLLDDFNSLGEKPLACQVVANNFNIIDDLQKAQNIKIERVGPIIKELSEKNYEIMTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q978R1</accession>
    <name>Q978R1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transport system permease protein P1P2A1A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1399304</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917588.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978R1"/>
    <dbReference type="STRING" id="273116.gene:9382162"/>
    <dbReference type="PaxDb" id="273116-14325593"/>
    <dbReference type="GeneID" id="1441470"/>
    <dbReference type="KEGG" id="tvo:TVG1399304"/>
    <dbReference type="eggNOG" id="arCOG00748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_5_3_2"/>
    <dbReference type="OrthoDB" id="312811at2157"/>
    <dbReference type="PhylomeDB" id="Q978R1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050366">
        <property type="entry name" value="BP-dependent_transpt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386:SF1">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DDPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="115"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="319" mass="35517" checksum="00B9DDAD90C7AC42" modified="2001-10-01" version="1">MAEVEVFKARRKEQKKRSARLEDFLFTLRLIVSNKIAFVGLLITLVYFAIAVIDYIDPRWLGVSNLSSALNFLHGKSYYPSATLVLPPTLSHGWYYWLGTTEYGIPILPDMLAALKVDLTLSVEIVLTGALIGLILGTVSAYYGGLIDEAMMRITDIFFSIPALILALAIAFIIGLTFTNIALAFIIVWWPIYARLGRSLTLSIKNMNFIEAANAAGSSSVRNIFYHVIPGVLSPLFVQFSLDIGTVVQLFAALEFLGVVPVDPFLPELGRMMVWGESYLIFGKWWAVLIPAIFLMIFTVSVSLLGDGLRDVLDPRQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q978R2</accession>
    <name>Q978R2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nickel import system ATP-binding protein NikD</fullName>
            <ecNumber evidence="10">7.2.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">TVG1397733</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Ni(2+)(out) + ATP + H2O = Ni(2+)(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15557"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15558"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">The complex is composed of two ATP-binding proteins (NikD and NikE), two transmembrane proteins (NikB and NikC) and a solute-binding protein (NikA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.2.2.11" evidence="10"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917587.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978R2"/>
    <dbReference type="STRING" id="273116.gene:9382161"/>
    <dbReference type="PaxDb" id="273116-14325592"/>
    <dbReference type="GeneID" id="25392648"/>
    <dbReference type="KEGG" id="tvo:TVG1397733"/>
    <dbReference type="eggNOG" id="arCOG00181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="PhylomeDB" id="Q978R2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015413">
        <property type="term" value="F:ABC-type nickel transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050388">
        <property type="entry name" value="ABC_Ni/Peptide_Import"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297:SF13">
        <property type="entry name" value="NICKEL ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT ATP-BINDING PROTEIN APPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="16"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB60495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="522" mass="59466" checksum="C72D12DC74553D58" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q978S1</accession>
    <name>Q978S1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TVG1388786 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1388786</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978S1"/>
    <dbReference type="STRING" id="273116.gene:9382152"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="891"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="CARDB" evidence="3">
        <location>
            <begin position="783"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="918"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="930"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="939" mass="102606" checksum="51E9714379FF9633" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q978S4</accession>
    <name>Q978S4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14 15">Shikimate kinase</fullName>
            <shortName evidence="14">SK</shortName>
            <ecNumber evidence="4 14 15">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">aroK</name>
        <name evidence="18" type="ORF">TVG1385893</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the GHMP kinase family. Archaeal shikimate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="4 14 15"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917576.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978S4"/>
    <dbReference type="STRING" id="273116.gene:9382149"/>
    <dbReference type="PaxDb" id="273116-14325580"/>
    <dbReference type="GeneID" id="1441458"/>
    <dbReference type="KEGG" id="tvo:TVG1385893"/>
    <dbReference type="eggNOG" id="arCOG01025">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073768_0_0_2"/>
    <dbReference type="OrthoDB" id="9602at2157"/>
    <dbReference type="PhylomeDB" id="Q978S4"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00370">
        <property type="entry name" value="Shik_kinase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010189">
        <property type="entry name" value="SK_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01920">
        <property type="entry name" value="Shik_kin_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF3">
        <property type="entry name" value="SHIKIMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005758">
        <property type="entry name" value="Shikimt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="16">
        <location>
            <begin position="47"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="17">
        <location>
            <begin position="210"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="NCBIfam" id="TIGR01920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAB60483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="268" mass="28969" checksum="6AC5E580086B98E5" modified="2001-10-01" version="1">MKFAEATTNGGISILSAFIDGLGGAFSIDLPLTATVYAGTRRREDRLMEAIRSEFGIKDQLTYKVKSSIPAGYGLKSSSALVLALAKASLLYKSVDIEDADLLVRLANISKAVGLSATGALDDLCQALYGGYCITDNKRMKIIKRGKLPELAVLVCARGETRPSRSINIRGNYFKVARSAENLAQRGFIFQAAVLNGFIYGTIFGIDLETVRKIEENGANYASQSGKGPAVFGLFTDPSDAKRAKMDIGFGIVTKINNHGIRYRIYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q978T3</accession>
    <name>Q978T3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Medium-chain acyl-CoA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1375308</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917567.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978T3"/>
    <dbReference type="STRING" id="273116.gene:9382140"/>
    <dbReference type="PaxDb" id="273116-14325571"/>
    <dbReference type="GeneID" id="1441449"/>
    <dbReference type="KEGG" id="tvo:TVG1375308"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_2"/>
    <dbReference type="OrthoDB" id="35688at2157"/>
    <dbReference type="PhylomeDB" id="Q978T3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004837">
        <property type="entry name" value="PRK06187.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859:SF4">
        <property type="entry name" value="BUTANOATE--COA LIGASE AAE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="18"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="442"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="529" mass="59621" checksum="B5D7B6286B34460B" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q978U1</accession>
    <name>Q978U1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3311 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1365320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917559.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978U1"/>
    <dbReference type="STRING" id="273116.gene:9382132"/>
    <dbReference type="PaxDb" id="273116-14325563"/>
    <dbReference type="GeneID" id="1441441"/>
    <dbReference type="KEGG" id="tvo:TVG1365320"/>
    <dbReference type="eggNOG" id="arCOG03694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183045_5_0_2"/>
    <dbReference type="OrthoDB" id="8235at2157"/>
    <dbReference type="PhylomeDB" id="Q978U1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021741">
        <property type="entry name" value="DUF3311"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11755">
        <property type="entry name" value="DUF3311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="66" mass="7429" checksum="8F905C5123DA5392" modified="2001-10-01" version="1">MNTKDIIVGILLIIPFVAYFAIPTYNRVNPAIGGLSFFYWYQTLWLAISALLFGIAAYIIDKGVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q978U5</accession>
    <name>Q978U5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purE</name>
        <name evidence="7" type="ORF">TVG1359744</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978U5"/>
    <dbReference type="STRING" id="273116.gene:9382128"/>
    <dbReference type="PaxDb" id="273116-14325559"/>
    <dbReference type="KEGG" id="tvo:TVG1359744"/>
    <dbReference type="eggNOG" id="arCOG02464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094982_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q978U5"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="6">
        <location>
            <begin position="5"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="159" mass="16777" checksum="9CB0831B55A4B4EE" modified="2001-10-01" version="1">MRFMPKVAVIMGSSSDYEVMKAAIDTLNEFGVDVISKVVSAHRTPEFMMQFAKEAASSGIEAIIAGAGGAAHLPGMVAAMTPLPVIGVPVPTKNLGGMDSLLSIVQMPSGVPVATVAIGNSKNAALLAIRILSIKYPDLMAKMSEFMESEKQRVLQEKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q978V1</accession>
    <name>PUR7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="1">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purC</name>
        <name type="ordered locus">TV1314</name>
        <name type="ORF">TVG1355049</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917549.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978V1"/>
    <dbReference type="SMR" id="Q978V1"/>
    <dbReference type="STRING" id="273116.gene:9382122"/>
    <dbReference type="PaxDb" id="273116-14325553"/>
    <dbReference type="GeneID" id="1441431"/>
    <dbReference type="KEGG" id="tvo:TVG1355049"/>
    <dbReference type="eggNOG" id="arCOG04421">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045637_1_0_2"/>
    <dbReference type="OrthoDB" id="10775at2157"/>
    <dbReference type="PhylomeDB" id="Q978V1"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01414">
        <property type="entry name" value="SAICAR_synt_Sc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010564">
        <property type="entry name" value="PRK13959.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100922" description="Phosphoribosylaminoimidazole-succinocarboxamide synthase">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <sequence length="294" mass="34317" checksum="72E9B3A1E9409725" modified="2001-10-01" version="1">MKLLTTGKVKDVYDDGDTLVFKFSNRISVFDKIIPNEIDNKGESLCRTSAFWFQLIESYGMKSHFIELIDNRTMRVRKFAVPNKVSLGSSNYVIPLEFITRYYVAGSLYDRIKEGKVKPMDIGLKHVPEYGEKLIDPIFEATTKREETDRLLTKKEAMEIGGLTLEDYCEIMEAVFKIDRRIDMEVSKRGLIHADGKKEIALDRERRIVVVDTFGTADEDRFWDEKEYDNGRVVELSKEMVRQYYRSIGYHDKLYYARENGLPEPDIPALSDDMVSKVSDLYRMMFEKITGQKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Z1</accession>
    <name>Q978Z1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1314728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917508.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Z1"/>
    <dbReference type="STRING" id="273116.gene:9382079"/>
    <dbReference type="PaxDb" id="273116-14325512"/>
    <dbReference type="DNASU" id="1441390"/>
    <dbReference type="GeneID" id="1441390"/>
    <dbReference type="KEGG" id="tvo:TVG1314728"/>
    <dbReference type="eggNOG" id="arCOG04349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113596_0_0_2"/>
    <dbReference type="OrthoDB" id="57193at2157"/>
    <dbReference type="PhylomeDB" id="Q978Z1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009324">
        <property type="entry name" value="DUF981"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06168">
        <property type="entry name" value="DUF981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="193" mass="21468" checksum="329576B22EFA128B" modified="2001-10-01" version="1">MSEFIDPLAVMLLGLGMGTALGAAYFLAHGLNKRDAVKSLVVPAFGVGFFDFVSGFIMSFTWPLPSSYNMLFGDPLLMFGLILMMSSYMEYRGFDLRFNSILLLFLGIYVLVASAGIVHYKLETGDDLLSSMGLYILDGIAAVLAPIMYIKPENGKYLYLLEFIILLLGTFVALFIGYEAIWGHLLDFIKYFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q979A7</accession>
    <name>Q979A7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1292898</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917489.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979A7"/>
    <dbReference type="PaxDb" id="273116-14325493"/>
    <dbReference type="DNASU" id="1441371"/>
    <dbReference type="GeneID" id="1441371"/>
    <dbReference type="KEGG" id="tvo:TVG1292898"/>
    <dbReference type="eggNOG" id="arCOG05400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131895_1_0_2"/>
    <dbReference type="OrthoDB" id="7914at2157"/>
    <dbReference type="PhylomeDB" id="Q979A7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07812">
        <property type="entry name" value="SRPBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15764" checksum="1998C6FD52895A18" modified="2001-10-01" version="1">MNIRFQEKIEVSADPEKVWKIVSKWEDIPNYWHGTTKILNLGNGLFKIRFAFPANGVMKFILLERERIVVEEYIKGPLRGIVRIGTCKENDSTLLSCDFDVKLIFPYSIFGKWSFNHFREGATHALVRIKERSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q979B5</accession>
    <name>Q979B5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1285178</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917480.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979B5"/>
    <dbReference type="SMR" id="Q979B5"/>
    <dbReference type="STRING" id="273116.gene:9382051"/>
    <dbReference type="PaxDb" id="273116-14325484"/>
    <dbReference type="DNASU" id="1441362"/>
    <dbReference type="GeneID" id="1441362"/>
    <dbReference type="KEGG" id="tvo:TVG1285178"/>
    <dbReference type="eggNOG" id="arCOG00683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_16_0_2"/>
    <dbReference type="OrthoDB" id="142001at2157"/>
    <dbReference type="PhylomeDB" id="Q979B5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="5">
        <location>
            <begin position="172"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="6">
        <location>
            <begin position="310"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="48580" checksum="2BA083A2041353CC" modified="2001-10-01" version="1">MKVRRTEQIYLKENDTISYMCHLSKNLYNQVNYILRQQFLKGEKLTGYRDLVKLFQEPSEIEDHNNYQRLPAQTAQWTVKKTVMAWYSFFKSMKAWKKHPDKFKGRPRLPGYKETDGEFLLVFTNQQCRIRDGILKFPKIMNMEVKTRLNENEIKLKEVRIVPQGTGYMIEIVYEKETADMPITKPKRAMGIDIGVRNLVTIGNNIHEIGIAVKAGLLKSINQYFNKELARYRSINDLQGNERKQTVKIRKLFMKRNRKVKDIMHKVSKSIVEYAKIMNIDTIVIGHNNRWKDSSNMGKKNNQNFVQLPFNMLIYQIKYKAEEIGITVIMQNEDYTSKCSFLDNESIEHHDTYMGKRIHRGIFRSATGKLIHADLQSSYNIIKKAIPEAFANGIEGIGLYPRSLSIRQMITSKGGC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q979C4</accession>
    <name>PFDB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Prefoldin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GimC subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pfdB</name>
        <name type="ordered locus">TV1237</name>
        <name type="ORF">TVG1278162</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917471.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979C4"/>
    <dbReference type="SMR" id="Q979C4"/>
    <dbReference type="STRING" id="273116.gene:9382042"/>
    <dbReference type="PaxDb" id="273116-14325475"/>
    <dbReference type="GeneID" id="1441353"/>
    <dbReference type="KEGG" id="tvo:TVG1278162"/>
    <dbReference type="eggNOG" id="arCOG01342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131909_0_0_2"/>
    <dbReference type="OrthoDB" id="56001at2157"/>
    <dbReference type="PhylomeDB" id="Q979C4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23162">
        <property type="entry name" value="Prefoldin_beta_GimC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00307">
        <property type="entry name" value="PfdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012713">
        <property type="entry name" value="PfdB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02338">
        <property type="entry name" value="gimC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000124873" description="Prefoldin subunit beta">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="124" mass="14642" checksum="82D0E28968A1993B" modified="2001-10-01" version="1">MVEPNISSYLQNQLKQAQELEENIEKIATQRYQLDLSLKEIEKTLQELNKIDDKTPVYRSIGSILYKVDDKKKLIDELEEQLELTKIRINTLDKQQKSLEEKYKELQAAIRERYNQDNKKGAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q979E5</accession>
    <name>Q979E5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cob(I)alamin adenosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1247214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917448.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979E5"/>
    <dbReference type="STRING" id="273116.gene:9382020"/>
    <dbReference type="PaxDb" id="273116-14325454"/>
    <dbReference type="GeneID" id="1441330"/>
    <dbReference type="KEGG" id="tvo:TVG1247214"/>
    <dbReference type="eggNOG" id="arCOG04678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088595_2_0_2"/>
    <dbReference type="OrthoDB" id="24392at2157"/>
    <dbReference type="PhylomeDB" id="Q979E5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003724">
        <property type="entry name" value="CblAdoTrfase_CobA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46638">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46638:SF1">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02572">
        <property type="entry name" value="CobA_CobO_BtuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015617">
        <property type="entry name" value="Adensltrnsf_CobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="196" mass="22500" checksum="0245A4000C5AFEE8" modified="2001-10-01" version="1">MQDGKLGLIDVFTGDGKGKTTAAFGLAFRALGWGYRVYVLQFMKLGTYGENKSAIKFDDGLIVDFVGMPYFIAWKGDIPDEDLKKVKNVLICEKGNPPSEYREAVLNHFNRSLEELKTGRWDIFIYDEINVALYYSLLKMDEVMRIFELKPEHTELVFTGRKMPKEIMDRADLVTEVSSPKHPYQRGILARRGIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q979G9</accession>
    <name>Q979G9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Non-heme chloroheme peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1221180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily. ABHD14 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979G9"/>
    <dbReference type="STRING" id="273116.gene:9381993"/>
    <dbReference type="ESTHER" id="thevo-TVG1221180">
        <property type="family name" value="CIB-CCG1-interacting-factor-B"/>
    </dbReference>
    <dbReference type="MEROPS" id="S33.010"/>
    <dbReference type="PaxDb" id="273116-14325430"/>
    <dbReference type="KEGG" id="tvo:TVG1221180"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_28_1_2"/>
    <dbReference type="PhylomeDB" id="Q979G9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46197:SF3">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46197">
        <property type="entry name" value="PROTEIN ABHD14B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="29"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="201" mass="22050" checksum="6C244468413BC99E" modified="2001-10-01" version="1">MKSLSRMSDQFVQVKSKKIHYIEEGSGIPIIMVHGARFSSETWVEVGTVSAVSSIPMRVISVDLPGYGRSEGGQWKDLRDFMNDFIDVLGIQKTALLGASLGGNVVASFAVSKPESVLALILVGAVGIRDLNADLSKLDGKPTLLIWGSKDTVSDRSNYETYMKYVKSSQFMNIGTNHACYFDDKNTFNNAVVSFLKKLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Q7</accession>
    <name>Q979Q7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1135520</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Q7"/>
    <dbReference type="STRING" id="273116.gene:9381901"/>
    <dbReference type="PaxDb" id="273116-14325341"/>
    <dbReference type="KEGG" id="tvo:TVG1135520"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059688_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050189">
        <property type="entry name" value="MFS_Efflux_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124:SF3">
        <property type="entry name" value="CHLORAMPHENICOL EFFLUX PUMP RV0191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124">
        <property type="entry name" value="PURINE EFFLUX PUMP PBUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="339" mass="37029" checksum="3B0A709E76591629" modified="2001-10-01" version="1">MTTSFYIGLALFQLVGGSLAHRIGNLKVSFIGLTILGTFGITSGLSSNFLELAISRFMAGVGSALFFSPALGIISDIVPPEKYGSYVSIYNGAFSFGAGAGIFAFGYFDTIIGWRLSLIIGGLLLYASDIAMVIALHGEEHRARGPLKDLAVVAKNPVVWILPVLTIGSAISETIMGQLFVYYLERFRYVPVVTASAIGSFFLFFGLPGGFISGMMIRKYQSKYAVTGFFIILASIFFMIPYEYNFAEILASVALFSVLSIYGFSYLYTVTSLVSKKAVSFSLSIVNFVQMIISAAVPYVYTYTIDSSSIYYAWYVMGIISLIPSLFILYLKINISNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Q8</accession>
    <name>Q979Q8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Exosome complex component Csl4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">csl4</name>
        <name evidence="4" type="ORF">TVG1133467</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Helpful for the interaction of the exosome with A-poor RNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with an hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. Interacts with DnaG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917335.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Q8"/>
    <dbReference type="STRING" id="273116.gene:9381900"/>
    <dbReference type="PaxDb" id="273116-14325340"/>
    <dbReference type="GeneID" id="1441217"/>
    <dbReference type="KEGG" id="tvo:TVG1133467"/>
    <dbReference type="eggNOG" id="arCOG00676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067135_1_1_2"/>
    <dbReference type="OrthoDB" id="6768at2157"/>
    <dbReference type="PhylomeDB" id="Q979Q8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00975">
        <property type="entry name" value="Exosome_Csl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030850">
        <property type="entry name" value="Exosome_Csl4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF034126">
        <property type="entry name" value="PRK09521.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0271">Exosome</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Exosome complex component N-terminal" evidence="3">
        <location>
            <begin position="8"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="202" mass="23000" checksum="A203BF366DF4CD90" modified="2001-10-01" version="1">MQDDKKVAFPGDMIATTEEYLPGKNTEEKNGEIIAVKFGQIIRDDINLLISVDTQKKEFTLKKGDIVYGQVVKGEQHRYIVRIVGAFQKGLGLREIDEEMQLSLGIARRPQSDFIAIGDLIRGYVIRTGDFPEISINGNHYGVISAVCLRCRQPLVKRGITLYCENCQRTEIRKMADDYGNVDIFNYENRIQNQVKANGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q979R0</accession>
    <name>Q979R0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small nuclear ribonucleoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1133061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917333.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979R0"/>
    <dbReference type="STRING" id="273116.gene:9381898"/>
    <dbReference type="PaxDb" id="273116-14325338"/>
    <dbReference type="GeneID" id="1441215"/>
    <dbReference type="KEGG" id="tvo:TVG1133061"/>
    <dbReference type="eggNOG" id="arCOG00998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_11_3_2"/>
    <dbReference type="PhylomeDB" id="Q979R0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="3">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="71" mass="8052" checksum="505870AD333FB783" modified="2001-10-01" version="1">MIMPMKLLEESVNKRVSLLLKDNRVLEGTLTGYDDYMNMVLDDVDENSENVSRKLGTVVIRGSNVVRIVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q979V0</accession>
    <name>Q979V0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF424 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1084391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917289.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979V0"/>
    <dbReference type="STRING" id="273116.gene:9381854"/>
    <dbReference type="PaxDb" id="273116-14325298"/>
    <dbReference type="GeneID" id="1441171"/>
    <dbReference type="KEGG" id="tvo:TVG1084391"/>
    <dbReference type="eggNOG" id="arCOG04051">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174522_0_0_2"/>
    <dbReference type="OrthoDB" id="18015at2157"/>
    <dbReference type="PhylomeDB" id="Q979V0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1860.10">
        <property type="entry name" value="uncharacterized conserved protein from methanopyrus kandleri domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007355">
        <property type="entry name" value="DUF424"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04242">
        <property type="entry name" value="DUF424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11335" checksum="6DCA8DC39FD08652" modified="2001-10-01" version="1">MNIVMKITEIRGEILLAAADSDILGKKFREKNLHIEVFPSFYGEVKVTDEMFISSLNMCTIANLVGKHTVELAIENGYIDKDNVLYIDGVPYAQYAKMFQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q979V5</accession>
    <name>TRPD_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpD</name>
        <name type="ordered locus">TV1055</name>
        <name type="ORF">TVG1080804</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054015.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979V5"/>
    <dbReference type="SMR" id="Q979V5"/>
    <dbReference type="STRING" id="273116.gene:9381849"/>
    <dbReference type="PaxDb" id="273116-14325293"/>
    <dbReference type="GeneID" id="1441166"/>
    <dbReference type="KEGG" id="tvo:TVG1080804"/>
    <dbReference type="eggNOG" id="arCOG02012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_3_1_2"/>
    <dbReference type="OrthoDB" id="8214at2157"/>
    <dbReference type="PhylomeDB" id="Q979V5"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.10">
        <property type="entry name" value="Transferase, Pyrimidine Nucleoside Phosphorylase, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154526" description="Anthranilate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="101"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <sequence length="330" mass="36388" checksum="A840C9DB461F8CB3" modified="2001-10-01" version="1">MMGIEDIVLGLNVDRESAKRLYEIISKEDCSDVCRAAVLTAFHFKGVTREEFMGFLDAIYKPTVSVDATDIVGTGGDKKNTINVSTASAIVAASLGIPVAKFGNRSSTGAYGSADFMADAGYSFPRDVEAALHRLKSNNFVFMLAPTFIPEFKIFSTVRKRLGFPTLFNYLGPVLNPLAPKKRVIGSASTEMMDVYSYILNELGYQAIIIRGQDGMDEITPFNFTDVIYVDGSVEKYVVDGRSISNDVRYDKISSQDPSLIRKFNFMGLKGENEDAAKFIALNTAPVLILNKKAGNYEDAYRLALEAIKSGQAYEKLEAIMHENKGLRHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q979W8</accession>
    <name>Q979W8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TVG1068265 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1068265</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_162009543.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979W8"/>
    <dbReference type="STRING" id="273116.gene:9381836"/>
    <dbReference type="PaxDb" id="273116-14325280"/>
    <dbReference type="GeneID" id="1441153"/>
    <dbReference type="KEGG" id="tvo:TVG1068265"/>
    <dbReference type="eggNOG" id="arCOG01812">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978679_0_0_2"/>
    <dbReference type="OrthoDB" id="57009at2157"/>
    <dbReference type="PhylomeDB" id="Q979W8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF2">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="112"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="279" mass="31877" checksum="7AEAA560FA632D4C" modified="2001-10-01" version="1">MPRKIFLFLQSQVARYVPRKTIDKLQDQLIKSQIELNAVSYLTLSIIVTFIFFILFAATSIFTYYFDRRYFFLVSAVFFLLAALVLYYSSSYPEIRQKWIARQVSLRITDFLAITYSLSLSGIPVDRILRDISNMDSLGEIAKESKRIVVMMDRLGYDFLTALEKAEKITASDDLSRIWAGLRLSILLDTDIVAYFRDRIDYYSRVQENKSKLSLDSLGLASESFVALVIAFPLLIFMTGSIISIKNLSTIFFVISLIIVLGASAVMFYFTSKITGDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A07</accession>
    <name>Q97A07_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA/pantothenate metabolism flavoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1025493</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917231.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A07"/>
    <dbReference type="STRING" id="273116.gene:9381796"/>
    <dbReference type="PaxDb" id="273116-14325220"/>
    <dbReference type="GeneID" id="1441113"/>
    <dbReference type="KEGG" id="tvo:TVG1025493"/>
    <dbReference type="eggNOG" id="arCOG01704">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_0_3_2"/>
    <dbReference type="OrthoDB" id="10536at2157"/>
    <dbReference type="PhylomeDB" id="Q97A07"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="3">
        <location>
            <begin position="17"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="4">
        <location>
            <begin position="190"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="393" mass="44565" checksum="ED399D1B104B6A6F" modified="2001-10-01" version="1">MLPYDKEMYARMLEGRTVIVATTASISIYRIPDLIRDLRREGAEVIVGMSESSEELVGPETMRWASEHDVVTKITGNVEHINLFINDKDKKALLIAPASYDTIGKMASGVTDTIPPLFFSFAFGYGVKTIVAPAMHRSMMENPINKENVEKLRRLGVDIVDPIYDEEKAKLSGNQCIIDHVCRAFYNKYDGKSILIISGRGEERIDPVRSITNDSTGYTGYWIARVAYRMGFSHITYIGNSIVDLPTYVNYRAAYTVDEFERLSEEEASNNYDYIVVNAALPDFKVIEKSEKKYSSNESHKLTLEPREKLVDKLSNISKGKIVAFRLTENIGEDPFKHFSKRIDMAVVNSYKTGPFGKTENEYRFVSKKFDVDLGRMPKPILAKNLVERLGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A08</accession>
    <name>Q97A08_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1023371</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulator TrmB family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917230.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A08"/>
    <dbReference type="STRING" id="273116.gene:9381795"/>
    <dbReference type="PaxDb" id="273116-14325219"/>
    <dbReference type="GeneID" id="1441112"/>
    <dbReference type="KEGG" id="tvo:TVG1023371"/>
    <dbReference type="eggNOG" id="arCOG02037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163833_0_0_2"/>
    <dbReference type="OrthoDB" id="30795at2157"/>
    <dbReference type="PhylomeDB" id="Q97A08"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09124">
        <property type="entry name" value="PLDc_like_TrmB_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021586">
        <property type="entry name" value="Tscrpt_reg_TrmB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11495">
        <property type="entry name" value="Regulator_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="3">
        <location>
            <begin position="15"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription regulator TrmB C-terminal" evidence="4">
        <location>
            <begin position="115"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="85"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27089" checksum="29E8D5DD4D722DA7" modified="2001-10-01" version="1">MEANNEKMQRIEEMLKMFGLSSYEAQGFAALVYHGVANADTIATTANIPRTSAYKVMESLVKKGLAKATEGRPRMFKPAKMEEIRDYYEDKLQQLFRDLKELEENAPSRGEPQLIYTINGTEKVMEKIEEMINMTEREIIISTPKIIEIRKQMKKPIENAVKRGARVIFVIPPNKRVPANVIVYRKEGLIATDIVSDQIRALIADPNLETCGYSDNPTLCMHVYQFINTMVNRPESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A21</accession>
    <name>Q97A21_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">D-3-phosphoglycerate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1010750</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917218.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A21"/>
    <dbReference type="STRING" id="273116.gene:9381781"/>
    <dbReference type="PaxDb" id="273116-14325206"/>
    <dbReference type="GeneID" id="1441100"/>
    <dbReference type="KEGG" id="tvo:TVG1010750"/>
    <dbReference type="eggNOG" id="arCOG01755">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_0_2"/>
    <dbReference type="OrthoDB" id="34275at2157"/>
    <dbReference type="PhylomeDB" id="Q97A21"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12165">
        <property type="entry name" value="2-Hacid_dh_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005027">
        <property type="entry name" value="PRK06436.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF178">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE YGL185C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="48"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="90"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="303" mass="34046" checksum="4D3E4431ADCF84E8" modified="2001-10-01" version="1">MHVYVNFPVEDKFKKIGESILDNYDVHWYPDYYDADAILIKNTYVIGKNTKMIQTLSAGVDHIDVQGIPENVVLCSNAGAYSISVAEHAFALLLARAKNVIENDMNMKNGKFIQSPTKLLYSKTLGILGYGGIGRRVAELGKAFGMDIAVFTRTLVKEGGVRFYKEPGPVLENSDVVVISLPLTDNTRGMINSSKLEMMKKDAMLINVARADIVVKEDILKHLISNPDFTYISDVWWNEPNITETNIKNTILSPHIAGGMSGEIMELAYEQAFRNIKAFFEGKPNNIVRKEEYKKRNERFVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A54</accession>
    <name>Q97A54_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Argininosuccinate lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0980504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917186.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A54"/>
    <dbReference type="STRING" id="273116.gene:9381748"/>
    <dbReference type="PaxDb" id="273116-14325173"/>
    <dbReference type="GeneID" id="1442034"/>
    <dbReference type="KEGG" id="tvo:TVG0980504"/>
    <dbReference type="eggNOG" id="arCOG01748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_0_2"/>
    <dbReference type="OrthoDB" id="27337at2157"/>
    <dbReference type="PhylomeDB" id="Q97A54"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005000">
        <property type="entry name" value="PRK06389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="1">
        <location>
            <begin position="28"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="437" mass="50191" checksum="C43931C19C7BE745" modified="2001-10-01" version="1">MKIWQGGAAKPSEYDPMEYLVKLDVSADTILEKYEIINLMAYHYELIKIGIINEEDGKCLLNALIKAYEGKITIDVKDEDVHTAIENWVKGLCPKNWENLRLFLSRNEQVHADMILYLLDSFYKMNKIFQSCIEKTIGVNGEGYLPGYTHFQQAMPFTFKSFMNSVLLLLERNILNVHDFFQKIRINVYGYGSGYGSPISAKFDKMGELLGLRYDHKNPIFLSSLYPQTLLEASQLIVTIMMPLSRLSSDTINYYSRGIMDIPDEFTTGSSLMPNKRNPDYLEMIQGLAAESVGVSSSIASIALNKQIGYHRDFQIAKDSIVFLIEKLIAHLDHLPDLLGRIEFFKEASERAVENSSYATMNAWNAFFKGTRWKEAYARIGNMVRENNELEKVDFETPTDNLDIANTRRIIYEEKYIVDSFLSVPERLKNLIRITES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A79</accession>
    <name>Q97A79_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0958399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917166.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A79"/>
    <dbReference type="STRING" id="273116.gene:9381723"/>
    <dbReference type="PaxDb" id="273116-14325148"/>
    <dbReference type="GeneID" id="1442014"/>
    <dbReference type="KEGG" id="tvo:TVG0958399"/>
    <dbReference type="eggNOG" id="arCOG02304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_2_1_2"/>
    <dbReference type="OrthoDB" id="28444at2157"/>
    <dbReference type="PhylomeDB" id="Q97A79"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="entry name" value="formyl-coa transferase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050483">
        <property type="entry name" value="CoA-transferase_III_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207:SF3">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="372" mass="41631" checksum="41C31E0DA9CACFB0" modified="2001-10-01" version="1">MRAIEIGHIVAGPTAGLLLSDLGFEVIKVERPKSGDIARSLTGTSSGAFAFYNRNKKSVTIDFKQKEGRDVLMKLIGTSDVLIDNLGYGALESAGLSYDEMKKHNPGLIYLSIRGYGNGPYEKRKSLDFPIEIHSGLAYMTGTLEKPMRVGASMIDMSAAMFGVIAVLDAIIEREKTGKGKQVTIGMFETALFFMGQHITTYQLIGKELKPINEDGFAWGIYDFFTTKDNKKVFVAVTTEDQWKKFCEVFSLSFCDEFPTNAIRYSKRDTLIPMLQDVMGKFDSTGLVERLESANISYAVLNKPWDLLNDPHASAKMISEEYCGRKIMVPYSPIGYNYVSDPPKLGDYTREVLRELGYSDYEIDYMEHNGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A80</accession>
    <name>Q97A80_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0956954</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A80"/>
    <dbReference type="STRING" id="273116.gene:9381722"/>
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    <dbReference type="KEGG" id="tvo:TVG0956954"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="90"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
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            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
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            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="446"/>
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    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="460" mass="51689" checksum="B73760EBB4F4F9A1" modified="2001-10-01" version="1">MFQSRMVDEKTYKGSVSDRLERIPFGSPQRNFLLMVTGGEWAETLMLLGNGVILALVASVLHFSHVLATLVVPTAFFLGEFVGSIFFGWLGDQRGRRTVFLYNLLVFSGGMIIAGLMSTAILIAIFVFIGGIGVGGEFPIVDTFTSEMMPGKERGKRLATVYTIAVTAGPVIAFLTYEMKIVAPPYVSWRILFWFMGIVGIAIWAVRTQLRESPRWLEVHGRYKEADAIVKDWEQRVIEEKHLQSLPEPKSETEVSERKSRYKDIFAPDLRRRTIMMLIFQFFQTGIFYGFTSLAPTFLLYKGITLVHSLEFSFIIYSGFFFGSIFNVFIIDKVERKWGIIASAVLAGVFGTLFGIIPNVTATVIFGFITTFVLWNFSNFYHTYQAEIFPTRVRPTAAGTVYSVSRISTSILVPFITAFFLPHGLLASFGLIWVFIAIVVFDLALLGPKTSKLRVEAIAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A91</accession>
    <name>Q97A91_THEVO</name>
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        <submittedName>
            <fullName evidence="2">Glucose-1-phosphate thymidylyltransferase</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="ORF">TVG0948272</name>
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    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
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            <taxon>Thermoplasmata</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883:SF2">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="4"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="351" mass="38549" checksum="0E34E867D156C96C" modified="2001-10-01" version="1">MYMKGIILHGGSGTRLRPLTYTDVKQLLPIAGKPISEYALENLIEIGIKNINIVIGSVGGLEVKKFYGDGSRWNVNISYTYQPEPLGIAHAIGLTKAFVGNDDFVVFLGDNYLQNGISNLYEDFTNAGSDGHLGLVPVDNPSQFGIAEVDNGKISKLVEKPKTPTSNLAIVGVYFLTPKVFESIDRLKPSKRGEYEITEAYQDMIDRGLKISYSIISGWFKDTGTVDDFLACNRLILDKLGDNGRRDNVSGRFDIHPTVKISSDSEVVGPCFIGEGTRIEHSYIGPYTSIGSNCIIKNAEIEDSIIMDGCEIDLLNENRIKKSLLGPNVRVVSGSKYGMRLVLGRDSKLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AA6</accession>
    <name>Q97AA6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2079 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0924334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AA6"/>
    <dbReference type="STRING" id="273116.gene:9381696"/>
    <dbReference type="PaxDb" id="273116-14325121"/>
    <dbReference type="KEGG" id="tvo:TVG0924334"/>
    <dbReference type="eggNOG" id="arCOG03812">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_500261_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018650">
        <property type="entry name" value="STSV1_Orf64"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09852">
        <property type="entry name" value="DUF2079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="62631" checksum="AA40CE403154634B" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AB6</accession>
    <name>Q97AB6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0911574</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AB6"/>
    <dbReference type="STRING" id="273116.gene:9381686"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325111"/>
    <dbReference type="DNASU" id="1441983"/>
    <dbReference type="GeneID" id="1441983"/>
    <dbReference type="KEGG" id="tvo:TVG0911574"/>
    <dbReference type="eggNOG" id="arCOG05431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782162_0_0_2"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="PhylomeDB" id="Q97AB6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
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    </dbReference>
    <dbReference type="Pfam" id="PF13439">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="14"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="195"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="43185" checksum="6A2492FDB586EA0D" modified="2001-10-01" version="1">MKIIILVRILWTAGAQKIAIAEAKQLTKMGYKVKLVFLRATESGRNLLNKLEDIDYEIFSGENYKPKLIYSFITGLFMLDRRGEGTVDYNLINKFARSVKKGDADYIICHDQWAGLAGLKIKRKLGIPYSVMMHEHVNGKYNVPVLGKIATKMERDVLKNADKIFAVTEKIANNVTSIYGFKCEPDLVGMTLQKTKNFHERDNIILATSTWGKDRSPDTYLDIIEAIPDFKLYFIGRWNLKSEFEDFKNNVYKRNLQEKVIINDNMPDEELDELYASSKFVIRFGGTKEYGLGTATVFAISHLTPVIIDSTLGISDIITKYGGGYIVDKNDINKISNFILENNNEINYEKLQNNLLNITREYTWERHCRILMNVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AC6</accession>
    <name>Q97AC6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0902620 protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">TVG0902620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567266_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AC6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="170" mass="19379" checksum="414D0756784A5EB4" modified="2001-10-01" version="1">MFSNDNSLSGKIYDTLKITNGNVVSFLEDDDLFTSNKLEIVFNLFKNENACYYHNASEIVDKFGHSLNIKNTDPYFNSSSISIRKDIIYRDIIKEIPKGVNTVLYINALDSGKEIVNGYEALTYYRAHKSETHFTGSLEEIASSDLNLAKLAVASFEILYKRLINKNRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AE5</accession>
    <name>Q97AE5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0885564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AE5"/>
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    <dbReference type="PaxDb" id="273116-14325082"/>
    <dbReference type="GeneID" id="1441957"/>
    <dbReference type="KEGG" id="tvo:TVG0885564"/>
    <dbReference type="eggNOG" id="arCOG04312">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107892_3_0_2"/>
    <dbReference type="OrthoDB" id="57216at2157"/>
    <dbReference type="PhylomeDB" id="Q97AE5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09879">
        <property type="entry name" value="PIN_VapC_AF0591-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
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        <property type="entry name" value="PIN_9"/>
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    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
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        <property type="entry name" value="PIN_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="7"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="121" mass="13590" checksum="00CC094F95BBE839" modified="2001-10-01" version="1">MKADTPNFILLDTNAIIYAIEKKIRLEDAVIEIPGVSRPAVPYCVIRELYGLAKKNVTARIAYQYAKKLEIIETDKYGDDGVIEAAMKTGAMVLTNDERLSRRLRETGIKVAMISGRKILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AG2</accession>
    <name>Q97AG2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pyruvate ferredoxin oxidoreductasse [POR] delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0870807</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AG2"/>
    <dbReference type="STRING" id="273116.gene:9381640"/>
    <dbReference type="PaxDb" id="273116-14325065"/>
    <dbReference type="KEGG" id="tvo:TVG0870807"/>
    <dbReference type="eggNOG" id="arCOG01605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_1_1_2"/>
    <dbReference type="PhylomeDB" id="Q97AG2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011898">
        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02179">
        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="95" mass="10998" checksum="AA25D4870542C1F3" modified="2001-10-01" version="1">MMVPVAEVSTRNHPTDSWRIQKPTIQYDKCIRCMICWKYCPDNAIEIKTGDEKAPNERVAKMEFPAIDYKFCKGCGICANECPEKCIDMEFEVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AI8</accession>
    <name>Q97AI8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0831891 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0831891</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917066.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AI8"/>
    <dbReference type="STRING" id="273116.gene:9381612"/>
    <dbReference type="PaxDb" id="273116-14325038"/>
    <dbReference type="DNASU" id="1441914"/>
    <dbReference type="GeneID" id="1441914"/>
    <dbReference type="KEGG" id="tvo:TVG0831891"/>
    <dbReference type="eggNOG" id="arCOG02610">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1182910_0_0_2"/>
    <dbReference type="OrthoDB" id="53244at2157"/>
    <dbReference type="PhylomeDB" id="Q97AI8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="250" mass="29082" checksum="E4DE8310FE150A94" modified="2001-10-01" version="1">MRVEDILDQLVMQSRDQDGAKYYISLINDMRIDRSVDPVKSIILEILSLCSDGRIDPWDVDLLKFAEIMNSFFGNSFIDFQFAGKAIADAWRVLRIKSDMSPKEQDRQMNDIGCEEPEDTDNFIPQNQVVIQLVPSVLTPQSRPVVLVDLIDEIRRKMYSSRLQKKKVDVHIEFDSSKLNPDRSEEMIEKTYRAIIESGKQKVFMSEIWGNTRRERAVFFLYSMFLFRDGRISLSQEEPNGDILIETNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AK1</accession>
    <name>Q97AK1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Aldehyde-ferredoxin oxidoreductase [AOR]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0808604</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>tungstopterin</name>
            <dbReference type="ChEBI" id="CHEBI:30402"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917053.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AK1"/>
    <dbReference type="STRING" id="273116.gene:9381599"/>
    <dbReference type="PaxDb" id="273116-14325025"/>
    <dbReference type="GeneID" id="1441901"/>
    <dbReference type="KEGG" id="tvo:TVG0808604"/>
    <dbReference type="eggNOG" id="arCOG00706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020364_1_0_2"/>
    <dbReference type="OrthoDB" id="30771at2157"/>
    <dbReference type="PhylomeDB" id="Q97AK1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013985">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF0">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="603" mass="65871" checksum="1E879D74BDBD1BDB" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AK2</accession>
    <name>Q97AK2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin converting factor subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0808580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917052.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AK2"/>
    <dbReference type="STRING" id="273116.gene:9381598"/>
    <dbReference type="PaxDb" id="273116-14325024"/>
    <dbReference type="GeneID" id="1441900"/>
    <dbReference type="KEGG" id="tvo:TVG0808580"/>
    <dbReference type="eggNOG" id="arCOG00536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_1_2_2"/>
    <dbReference type="OrthoDB" id="98357at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17505">
        <property type="entry name" value="Ubl_SAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010038">
        <property type="entry name" value="MoaD_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054834">
        <property type="entry name" value="SAMP1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052045">
        <property type="entry name" value="Sulfur_Carrier/Prot_Modifier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01687">
        <property type="entry name" value="moaD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041918">
        <property type="entry name" value="SAMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38031:SF1">
        <property type="entry name" value="SULFUR CARRIER PROTEIN CYSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38031">
        <property type="entry name" value="SULFUR CARRIER PROTEIN SLR0821-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="91" mass="10193" checksum="D2F633A893F41BC6" modified="2001-10-01" version="1">MSVTVRYYANLRSVTGKKLEEFDGIKNIDDLISLLNQEYGEKFRKLMYNGNELYPNVIILLNGNNINSIEGLKTPLKDGDNIDVFPPVAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AK4</accession>
    <name>Q97AK4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsZ</name>
        <name evidence="9" type="ORF">TVG0806423</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the FtsZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AK4"/>
    <dbReference type="STRING" id="273116.gene:9381596"/>
    <dbReference type="PaxDb" id="273116-14325022"/>
    <dbReference type="KEGG" id="tvo:TVG0806423"/>
    <dbReference type="eggNOG" id="arCOG02201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_2"/>
    <dbReference type="PhylomeDB" id="Q97AK4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF3">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN FSZA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="7">
        <location>
            <begin position="30"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="8">
        <location>
            <begin position="223"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="347" mass="37421" checksum="5CC382D1BFA82331" modified="2001-10-01" version="1">MCMDNSNNSEEDIWTNDEKLQKIIEELNFRIKVFGFGGSGSNTINRLMRENLSGVKLIACNTDAAHLLRIRAHSKILLGKNLTRGLGAGADPSVGEMAAKESESEILKQIDETSIVFITAGLGGGTGTGAAPYVAKLAKDRGALTISFATLPFSTEGFVRMKNAYEGVRKLVKNSDAAVIIPNDKLIEKFNDVPVYKAFKFEDEVIATGIKGITDLIMSTGTINLDFNDLRKVMKDAGYSAIGMGSSSQAVNDRIVEALEKALDSPFMDVDISKAKGAIINVTGGRDLQLQEAQQAADILKKKIARDATIMWGTVVDESIRSSVKILVIVAGVKPNFKFEPGIEEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AL0</accession>
    <name>Q97AL0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0800897</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AL0"/>
    <dbReference type="STRING" id="273116.gene:9381590"/>
    <dbReference type="PaxDb" id="273116-14325016"/>
    <dbReference type="KEGG" id="tvo:TVG0800897"/>
    <dbReference type="eggNOG" id="arCOG06050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881171_0_0_2"/>
    <dbReference type="OrthoDB" id="57161at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020948">
        <property type="entry name" value="P_starv_induced_PsiE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06146">
        <property type="entry name" value="PsiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="143" mass="16093" checksum="A4846EDED56BAD84" modified="2001-10-01" version="1">MIIFKYNTHIQIVNDPTQYVVKYFGYIIEAILILAIVFDLCFSIYQLIILRSSGLEELVYSAVGNAFFALVLLELFEGVHDFIVKSPQGLRHVVEAGLSYIIREIIIDISTGIKDIYSLSALAIVIIALSVSLYFLVLTKNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AL2</accession>
    <name>Q97AL2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Benzoylformate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0799969</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917042.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AL2"/>
    <dbReference type="STRING" id="273116.gene:9381588"/>
    <dbReference type="PaxDb" id="273116-14325014"/>
    <dbReference type="GeneID" id="1441890"/>
    <dbReference type="KEGG" id="tvo:TVG0799969"/>
    <dbReference type="eggNOG" id="arCOG03656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_2"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="PhylomeDB" id="Q97AL2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02002">
        <property type="entry name" value="TPP_BFDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="4">
        <location>
            <begin position="183"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="5">
        <location>
            <begin position="362"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="500" mass="55880" checksum="65CA3ACF7100C0C4" modified="2001-10-01" version="1">MKASEIFAKTLEEHELYPVYGNPGTTEIPMLSFVKDYRLTLHDSISVAMADAVSQYSGRPAVVNLHSLPGISNSMAFIYSAFKNNSPIIITAGQQDRRHLIYDPLLSGDQVDMVSPYVKYAYEVRMPSEIPEAMKRAYEIAMTPPYGPVFLSFPMDLMDEDAEYHSSEFYSTPGIAIDEDIVKSIMDRVNSARKVAIVYGYEVDLYGAMDLAREFSTKLNVPVFSEPWSQGSCCYRDQNFVRDLPPAMAAIDAILSTFDLVLFIGSDIFVYPYTPAEALRNVEAYFISTRPTHFAGHSIISDPRSFLSVALKYAKKKENYKIAMGGGNEVAEEEIKMIHDYFKGYTAIDEAVTASGAVRKYFADGPKSYYTSRGGPLGWGDAATVGISALQERTLGIIGDGAFMYTVQSLWTASRYGMPSKFVVLRNNAYNILRSYSKAFGYGIEDADFLKLEYDPVKVSEGLGVEARVYASIDDIKWLKEEKKAKVLVIDTDQRIPEMF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-07" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AL5</accession>
    <name>NFI_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Bifunctional methyltransferase/endonuclease</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName>Probable methylated-DNA--protein-cysteine methyltransferase</fullName>
                <ecNumber>2.1.1.-</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>O-6-methylbase-DNA-alkyltransferase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName>Endonuclease V</fullName>
                <ecNumber>3.1.21.7</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>Deoxyinosine 3'endonuclease</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>Deoxyribonuclease V</fullName>
                <shortName>DNase V</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name type="ordered locus">TV0795</name>
        <name type="ORF">TVG0795955</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.</text>
            <dbReference type="EC" id="3.1.21.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the MGMT family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the endonuclease V family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EC" id="3.1.21.7"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917039.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AL5"/>
    <dbReference type="SMR" id="Q97AL5"/>
    <dbReference type="STRING" id="273116.gene:9381585"/>
    <dbReference type="PaxDb" id="273116-14325011"/>
    <dbReference type="DNASU" id="1441887"/>
    <dbReference type="GeneID" id="1441887"/>
    <dbReference type="KEGG" id="tvo:TVG0795955"/>
    <dbReference type="eggNOG" id="arCOG00929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047631_1_1_2"/>
    <dbReference type="OrthoDB" id="372118at2157"/>
    <dbReference type="PhylomeDB" id="Q97AL5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043737">
        <property type="term" value="F:deoxyribonuclease V activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007581">
        <property type="entry name" value="Endonuclease-V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511:SF1">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04493">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000159700" description="Bifunctional methyltransferase/endonuclease">
        <location>
            <begin position="1"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Probable methylated-DNA--protein-cysteine methyltransferase">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Endonuclease V">
        <location>
            <begin position="87"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with target DNA" evidence="1">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="321" mass="36049" checksum="FC3220C1E37B7ACD" modified="2001-10-01" version="1">MLSVDYENFDLISYLYNLVRQIPDGMVSTYGDLAEALGDPVAARSVGFMLSINKEPDYIPCYKVVPHDGSVGNYTHPLGSAEKMRRLIRDGITITNGHISNFEKVRFREFKTDYPLRKLRELQFKIGRLYDDRNDYSLDTIAAFDVSYKETRGYASKVVYNKGKIGAYVYSSDSMFPYIPGYLAFKEFKFIRALYDNETMILIDGNGILHPRFAGLATHAGVSLKTASIGIAKHLINCTVKGSDLLISGVVAGKMIGHHTIVSPGNRINVEEAGRLIEQREGKEMRSLLRLAHNLTRLHIETNGNIARYDFSTRNTAIQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AQ6</accession>
    <name>Q97AQ6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription factor S</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription elongation factor IIS/RNA polymerase subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">TVG0759000</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AQ6"/>
    <dbReference type="STRING" id="273116.gene:9381543"/>
    <dbReference type="PaxDb" id="273116-14324969"/>
    <dbReference type="KEGG" id="tvo:TVG0759000"/>
    <dbReference type="eggNOG" id="arCOG00579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093932_3_2_2"/>
    <dbReference type="PhylomeDB" id="Q97AQ6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10511">
        <property type="entry name" value="Zn-ribbon_TFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006288">
        <property type="entry name" value="TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01384">
        <property type="entry name" value="TFS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="13">
        <location>
            <begin position="64"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB59895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="106" mass="12213" checksum="6C8A75D143606399" modified="2001-10-01" version="1">MQSSLSMFCPKCGSLMTPVNGRYVCPSCGYEVSKKKEDLKIVNKSNDKETIMIREEVSAEPLDSDAICPRCHHKGARYVLKQTRSADEPETKFYTCEECGYRWREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AT5</accession>
    <name>Q97AT5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0731430 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0731430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AT5"/>
    <dbReference type="PaxDb" id="273116-14324940"/>
    <dbReference type="KEGG" id="tvo:TVG0731430"/>
    <dbReference type="HOGENOM" id="CLU_2091429_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="116" mass="14103" checksum="9A99037EDB6AD982" modified="2001-10-01" version="1">MYIFQLILLRNGFFVDINYILLLFKCEPIKRLLYVIICSVLICDFFTIHFIDYISNATRHRTKFRTMLIKLVQDIMMVNRNRLHRRFKFILYRSLTLMTDGRSSFPYHLEQAKLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AU4</accession>
    <name>Q97AU4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0722263</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916973.1">
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    </dbReference>
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    <dbReference type="STRING" id="273116.gene:9381504"/>
    <dbReference type="PaxDb" id="273116-14324931"/>
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    <dbReference type="KEGG" id="tvo:TVG0722263"/>
    <dbReference type="eggNOG" id="arCOG01947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087840_1_2_2"/>
    <dbReference type="OrthoDB" id="121309at2157"/>
    <dbReference type="PhylomeDB" id="Q97AU4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38825">
        <property type="entry name" value="LYSINE EXPORTER PROTEIN (LYSE/YGGA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38825:SF2">
        <property type="entry name" value="LYSINE TRANSPORTER LYSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="6"/>
            <end position="29"/>
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    </feature>
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        <location>
            <begin position="41"/>
            <end position="60"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="87"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="118"/>
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    </feature>
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        <location>
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            <end position="152"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="186" mass="20558" checksum="809E99927C880C80" modified="2001-10-01" version="1">MEILTYLIGVLLGLSLTAPPGPVNSIISVESVRSKIHGSSVGAGAMTADIIFFIIVYSFGNIVPKSALYALYIVGSIVMFYFAYSTLKKSSRYKTRRGNYFVGLLVGISNPFQIIWWVTAGLFVVNHMSLIGVFGLFSGVVLWITIFPFFVNKYAKRYEKYVRIASSVVLISFGVYILIVGIKVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <end position="83"/>
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        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59856.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="130" mass="14878" checksum="599C63E6A5A4F2FE" modified="2001-10-01" version="1">MMRNEHVAKGITGVSWYDFKQKLKYKAECGNMEFIEIGRFDPSFEIWSRCGWIDYNLKLSDRIFRCENCGLVIDRDLNTAINISNMELKKVGKGIPEFTPVDIATAADPTIRTGTASSRSMNREPPKPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AV1</accession>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59850.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="144" mass="16874" checksum="66346515522C856E" modified="2001-10-01" version="1">MLRMVLQENHELVQEKYKVSKPNGNDNTDVIPFVDLTILMKHGRSYNFRSRSLKRDKFAEWYQVIVTMKNIDKPMTKTEIINRSMLNFRRGENIINCMENTGLLDTNIVGKRKYYSLSELGDLILNVACIIASFIPYEEHNDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AV2</accession>
    <name>Q97AV2_THEVO</name>
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        <submittedName>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
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    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59849.1"/>
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</entry>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Koike H."/>
                <person name="Makino S."/>
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                <person name="Kawashima-Ohya Y."/>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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            <dbReference type="PubMed" id="11121031"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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            <end position="196"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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        <source>
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        <source>
            <dbReference type="EMBL" id="BAB59820.1"/>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="196" mass="22781" checksum="BE25CF13715C420D" modified="2001-10-01" version="1">MRSDKVDKTKKYPPAYYRYREKHVTLSILLSNDFKEFLDHYKKSGMTYPEIVKDALEKGKRMSEILQAEANDKLALEMDAFVKASDLAYKERLEKELATMEETIRARVKESVLSAIAISTERIQKAILNSFAFDEHGKQLIRRIFERYRFTWNSVEAKPQQHGAISGAHTAPPQDEDEDDIEDVDDDDQYTPGWLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B16</accession>
    <name>Q97B16_THEVO</name>
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        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="5">Multi-pass membrane protein</topology>
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        <subcellularLocation>
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        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
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    <dbReference type="OrthoDB" id="12267at2157"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="TSUP/Inactive_protease-like"/>
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    <dbReference type="PANTHER" id="PTHR43701">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN MJ0441-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701:SF2">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN YJNA-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="278" mass="29571" checksum="F2127778C13FB6CA" modified="2001-10-01" version="1">MDITLLQYIYSIISGVLVGFSLGLIGGGGSILAIPLLIYFVGIRDPHLVIGTTALAVGINALINVYSHLRKKNVNFKIGTAFTVFGVIGVLIGTTLGLITPGGELLFLFSFLMIAIGLYMFLTKCRTCKNFDALPKDDPPDPPDDPVHFSNIRKKFGQFKVYIFGLLAGFASGYFGIGGGFLIVPALLYSADITMNIAVGTSLLSVGTFGLVTALRYGIAGEVLVPIALLYVLGGIFGGFAGARVSTSMKRSTLRRFFSIIIVVVGIYMAIESYSAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B18</accession>
    <name>Q97B18_THEVO</name>
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        <submittedName>
            <fullName evidence="2">TVG0634229 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0634229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
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            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916900.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B18"/>
    <dbReference type="PaxDb" id="273116-14324857"/>
    <dbReference type="GeneID" id="1441748"/>
    <dbReference type="KEGG" id="tvo:TVG0634229"/>
    <dbReference type="eggNOG" id="arCOG07385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2271166_0_0_2"/>
    <dbReference type="OrthoDB" id="56312at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="106" mass="12440" checksum="1A60B6A505F8FBAC" modified="2001-10-01" version="1">MTVRIVLDLSDDEKRIIDLIRVQGETYVDTIKRIINNYFEYQLVKEAVDKLYDKLGVNEKENRKNDGDEKQVSKEKAQVKIPVKTGNPVDADKLAEFKKNLESRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B57</accession>
    <name>Q97B57_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0592854 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0592854</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmatota</taxon>
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            <taxon>Thermoplasmataceae</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B57"/>
    <dbReference type="STRING" id="273116.gene:9381389"/>
    <dbReference type="PaxDb" id="273116-14324816"/>
    <dbReference type="KEGG" id="tvo:TVG0592854"/>
    <dbReference type="eggNOG" id="arCOG05401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130233_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97B57"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="154" mass="17565" checksum="3795E1AD48933A39" modified="2001-10-01" version="1">MIMAEKETKEEETKKITISVKGIRDDLYKNVTDLAKHTGKTIGEITNDAYRTFIGSIEGIRNISQNFVEGAKSALPRYIDNFKKLEVTAEDLKDFGQKVAFRNIDELTFKNVTDEDIQKYVAFIDGVKILRIPKTVKKSTIIRISTFVDEIVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B75</accession>
    <name>Q97B75_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0571216 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0571216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916841.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B75"/>
    <dbReference type="STRING" id="273116.gene:9381369"/>
    <dbReference type="PaxDb" id="273116-14324797"/>
    <dbReference type="GeneID" id="1441689"/>
    <dbReference type="KEGG" id="tvo:TVG0571216"/>
    <dbReference type="eggNOG" id="arCOG07378">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076319_0_0_2"/>
    <dbReference type="OrthoDB" id="56021at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="232" mass="26387" checksum="5D0DB96C76F4004A" modified="2001-10-01" version="1">MLYLYSYHQPKIGNTENEYEDAFAYDLNTGRFAMADGSSESVFSNIWAKALVDEFVSGDYILKIDTLVEKAASKWQSKLPWKEMKWFVKNKIMMGALSTFLGLVVSDEKFSAVAVGDTCLFVFSSGGYRSFPITKLSDFGNSPKLVWTGNPVLRARRQIKWVNFLEGQTDGVVLVMATDSLSYWILKNAENDPWLTLLNSKGHEKEFIDDLIEKKKIKNDDITFTIISQNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B77</accession>
    <name>Q97B77_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mg-protoporphyrin IX monomethyl ester oxidative cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0569632</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916839.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B77"/>
    <dbReference type="STRING" id="273116.gene:9381367"/>
    <dbReference type="PaxDb" id="273116-14324795"/>
    <dbReference type="GeneID" id="1441687"/>
    <dbReference type="KEGG" id="tvo:TVG0569632"/>
    <dbReference type="eggNOG" id="arCOG01356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021572_4_4_2"/>
    <dbReference type="OrthoDB" id="2305at2157"/>
    <dbReference type="PhylomeDB" id="Q97B77"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02068">
        <property type="entry name" value="radical_SAM_B12_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051198">
        <property type="entry name" value="BchE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034466">
        <property type="entry name" value="Methyltransferase_Class_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409">
        <property type="entry name" value="ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409:SF7">
        <property type="entry name" value="BLL1977 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01123">
        <property type="entry name" value="methyltransferase_(Class_B)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="B12-binding" evidence="8">
        <location>
            <begin position="12"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="190"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="529" mass="60121" checksum="2467E07F77B731CC" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B80</accession>
    <name>Q97B80_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="4 9">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG0566451</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4 9"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B80"/>
    <dbReference type="STRING" id="273116.gene:9381363"/>
    <dbReference type="PaxDb" id="273116-14324792"/>
    <dbReference type="KEGG" id="tvo:TVG0566451"/>
    <dbReference type="eggNOG" id="arCOG00980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073526_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q97B80"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054016">
        <property type="entry name" value="FKBP26_IF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048261">
        <property type="entry name" value="SlpA/SlyD-like_ins_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861:SF3">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22199">
        <property type="entry name" value="FKBP26_IF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="11">
        <location>
            <begin position="13"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB59719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="268" mass="30408" checksum="666A6018EDCC9618" modified="2001-10-01" version="1">MRSKTKVKCLNTGDFIKIEYEMRVGDDKKLVATSKEELAKENNIYDEKQKYGDIVVIVGQEGLFKEINESFKNAQVGQELEVEIPPEDAFGQRLPSNIKVHTMREFQRLNIDPRVGMEVEINRKIGRIISVTPGRVLVDYNHKWAGKKVYYKYKINGTITDTAEKVKAIIANNYDPEGFNVSVDDSIRIEIPENAKFSVAWLDAKYTIVNTARKYIPGLDILITEKYEKGKGEEQNKTETTAEQPAAEQRETPAEAKGSGQEETNAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B89</accession>
    <name>Q97B89_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdopterin converting factor subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0556526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916827.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B89"/>
    <dbReference type="STRING" id="273116.gene:9381354"/>
    <dbReference type="PaxDb" id="273116-14324783"/>
    <dbReference type="GeneID" id="1441085"/>
    <dbReference type="KEGG" id="tvo:TVG0556526"/>
    <dbReference type="eggNOG" id="arCOG00536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_4_1_2"/>
    <dbReference type="OrthoDB" id="98357at2157"/>
    <dbReference type="PhylomeDB" id="Q97B89"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990133">
        <property type="term" value="C:molybdopterin adenylyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00754">
        <property type="entry name" value="Ubl_MoaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010038">
        <property type="entry name" value="MoaD_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044672">
        <property type="entry name" value="MOCS2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01687">
        <property type="entry name" value="moaD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359:SF1">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="90" mass="10212" checksum="0C8C86309B9724CC" modified="2001-10-01" version="1">MGIIQGDVLDLKVRVRYFAYFKEKVGKDEEYFELSPGSNVGTLINEVMKKYKNIFNPKDTLIAVNYKYVDQNYNIAEGDTVAIMPHVSGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-15" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B93</accession>
    <name>RPO3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo3</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo3</name>
        <name evidence="1" type="synonym">rpoD</name>
        <name type="ordered locus">TV0564</name>
        <name type="ORF">TVG0551901</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916823.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B93"/>
    <dbReference type="SMR" id="Q97B93"/>
    <dbReference type="STRING" id="273116.gene:9381349"/>
    <dbReference type="PaxDb" id="273116-14324779"/>
    <dbReference type="GeneID" id="1441081"/>
    <dbReference type="KEGG" id="tvo:TVG0551901"/>
    <dbReference type="eggNOG" id="arCOG04241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_3_1_2"/>
    <dbReference type="OrthoDB" id="84933at2157"/>
    <dbReference type="PhylomeDB" id="Q97B93"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07030">
        <property type="entry name" value="RNAP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000132768" description="DNA-directed RNA polymerase subunit Rpo3">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00320"/>
        </source>
    </evidence>
    <sequence length="271" mass="30296" checksum="C76EA40B23C6AD30" modified="2001-10-01" version="1">MEVKIFKLSDKYMSFEIDGITPSQANALRRTLINDIPKLAIENVTFHHGEIRDAEGNVYDSSLPLFDEMVAHRLGLIPLKTDLSLNFRDQCSCGGKGCSLCTVTYSINKIGPASVMSGDIQAISHPDLVPVDPDIPIVKLGAKQAILITAEAILGTAKEHAKWQVTSGVAYKYHREFEVNKKLFEDWAKIKERCPKSVLSEDENTIVFTDDYGCNDLSILFESDGVQIKEDDSRFIFHFETDGSLTAEETLSYALNRLMDRWGILVESLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B97</accession>
    <name>Q97B97_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycerol-3-phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0549499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916819.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B97"/>
    <dbReference type="STRING" id="273116.gene:9381345"/>
    <dbReference type="PaxDb" id="273116-14324775"/>
    <dbReference type="GeneID" id="1441077"/>
    <dbReference type="KEGG" id="tvo:TVG0549499"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_705185_0_0_2"/>
    <dbReference type="OrthoDB" id="29061at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17478">
        <property type="entry name" value="MFS_FsR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43129">
        <property type="entry name" value="FOSMIDOMYCIN RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43129:SF1">
        <property type="entry name" value="FOSMIDOMYCIN RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="15"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="415" mass="45322" checksum="F9599DA70C02C7BD" modified="2001-10-01" version="1">MESSMKFGRPERIKALTFTSLGHFTNDFNILLFSVLITYYHRYFGISLALLGAIAIIYNIISGFLSTPIGRYADKTRAYRGLMMLGIFILGVSLFLFGFSFLSASFVIWIMLIAVIFLGIGQAFYHPLGGSILNVTYGERAASALGINGAFGSIGRSALPVILVPLILLVGKAYALFVAGTYMFVAGVVIYAGLSFLKKREIPNRSREASSAANKETYLRYRGVLAIIVAMIFIRAMFLTGTTTYISQFVLDRVKSEILMSYILTISFLTAVIGQPVFGRLTEKYGGKWSISITTILSTLFFIFFMLSGDNAVLDTVTYAAFVFMAFTGFPVLMGYVNQIVPNNISTTANGLVWGLGNTVGGGIGIAVMSIFLNLHFTLTSVMWIMIIFGVISAFFIPLIPGVKRSAELKSGSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BB0</accession>
    <name>Q97BB0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0537452</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916805.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BB0"/>
    <dbReference type="STRING" id="273116.gene:9381332"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324762"/>
    <dbReference type="DNASU" id="1441063"/>
    <dbReference type="GeneID" id="1441063"/>
    <dbReference type="KEGG" id="tvo:TVG0537452"/>
    <dbReference type="eggNOG" id="arCOG01381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_19_1_2"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="PhylomeDB" id="Q97BB0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630:SF1">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="3">
        <location>
            <begin position="7"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="250" mass="28261" checksum="DD515DCEF1C29150" modified="2001-10-01" version="1">MDEEGASVVITIKNEAKNIQKLLDSLFNNDYEHFEVVVVDDMSNDGTEGIVKKYDKVKYIRTRCSRGEGRNIGAMNSSFENILFTDGDAIVSNGWIGGMVSVLNNGFEIAIGKTVYTGKKRYIQDRVKIYFNGIEITAPSVNLAYKKRAFFSLGGFDKRFVTAEDIDLNLRAAMSGFKFGFCNECVVYAKVRDSFISFLKQAFWNGYGRAQLEEKHPGMLKKTDIRLGHNFYDFMHMSAGAAGYLLYKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BF0</accession>
    <name>Q97BF0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="9">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA wyosine derivatives biosynthesis protein Taw1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">taw1</name>
        <name evidence="11" type="ORF">TVG0493454</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="9"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916764.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BF0"/>
    <dbReference type="STRING" id="273116.gene:9381289"/>
    <dbReference type="PaxDb" id="273116-14324721"/>
    <dbReference type="GeneID" id="1441022"/>
    <dbReference type="KEGG" id="tvo:TVG0493454"/>
    <dbReference type="eggNOG" id="arCOG04174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007952_3_0_2"/>
    <dbReference type="OrthoDB" id="68499at2157"/>
    <dbReference type="PhylomeDB" id="Q97BF0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01921">
        <property type="entry name" value="TYW1_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023993">
        <property type="entry name" value="TYW1_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03972">
        <property type="entry name" value="rSAM_TYW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="45"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="312" mass="35178" checksum="13C28ABB55D7FBF7" modified="2001-10-01" version="1">MNNLYADIYKKEHYGLVGKHSAVKVCHWTKDELLGGQGCYKNTFYGIKSHQCIQMTPALNACTENCAFCWRFQGFDSMHISDEDDPEFILEESIKQHLKLISGFKGNPKVSKEIWEEATHPKHMAISLTGEPTLYSRLGELIAAAKKRGISTFLVTNGTLPMVLEKLDPLPTQLYVTVAAPNKQIFNQLLQPSIGNGWENLNKTLELLPSLNTRKVIRHTLVRDVNMPYVEEYAKLDSKGDPDFIEAKGYVHVGYSISKLSTNNMPPHSEILEFSHSLSEKLGYQFVADRVESRVAMIAKDPSKAKIDFSNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BF5</accession>
    <name>KDPC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Potassium-transporting ATPase KdpC subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP phosphohydrolase [potassium-transporting] C chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Potassium-binding and translocating subunit C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Potassium-translocating ATPase C chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kdpC</name>
        <name type="ordered locus">TV0501</name>
        <name type="ORF">TVG0489908</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit acts as a catalytic chaperone that increases the ATP-binding affinity of the ATP-hydrolyzing subunit KdpB by the formation of a transient KdpB/KdpC/ATP ternary complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The system is composed of three essential subunits: KdpA, KdpB and KdpC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KdpC family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916759.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BF5"/>
    <dbReference type="SMR" id="Q97BF5"/>
    <dbReference type="STRING" id="273116.gene:9381284"/>
    <dbReference type="PaxDb" id="273116-14324716"/>
    <dbReference type="GeneID" id="1441017"/>
    <dbReference type="KEGG" id="tvo:TVG0489908"/>
    <dbReference type="eggNOG" id="arCOG04805">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077094_1_0_2"/>
    <dbReference type="OrthoDB" id="8035at2157"/>
    <dbReference type="PhylomeDB" id="Q97BF5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008556">
        <property type="term" value="F:P-type potassium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00276">
        <property type="entry name" value="KdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003820">
        <property type="entry name" value="KdpC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001454">
        <property type="entry name" value="PRK00315.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30042">
        <property type="entry name" value="POTASSIUM-TRANSPORTING ATPASE C CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30042:SF2">
        <property type="entry name" value="POTASSIUM-TRANSPORTING ATPASE KDPC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02669">
        <property type="entry name" value="KdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001296">
        <property type="entry name" value="K_ATPase_KdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-0633">Potassium transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000197027" description="Potassium-transporting ATPase KdpC subunit">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00276"/>
        </source>
    </evidence>
    <sequence length="209" mass="23280" checksum="92F13919AE616DF8" modified="2001-10-01" version="1">MVKSPLKALVISLVFLFVLGFLFPTVTSLITEKALPNQSEGQPIKIDGKVYGSYLLAEAFNSSIFFHPRPSAIGYNLSESGSYPYSLGNPEVLNLTEKYLDEFLKDNPGINASQIPYAMLSYSASGLDPNIPLQGALIQIPRISIALHSITNNSVSLWENYLNDLVNKYTTQNFPFFGSYYVNIMYLNVNILEYLMNNGYIKSLNSIPP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BF7</accession>
    <name>KDPA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Potassium-transporting ATPase potassium-binding subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP phosphohydrolase [potassium-transporting] A chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Potassium-binding and translocating subunit A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Potassium-translocating ATPase A chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kdpA</name>
        <name type="ordered locus">TV0499</name>
        <name type="ORF">TVG0487282</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit binds the extracellular potassium ions and delivers the ions to the membrane domain of KdpB through an intramembrane tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The system is composed of three essential subunits: KdpA, KdpB and KdpC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KdpA family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916757.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BF7"/>
    <dbReference type="SMR" id="Q97BF7"/>
    <dbReference type="STRING" id="273116.gene:9381282"/>
    <dbReference type="PaxDb" id="273116-14324714"/>
    <dbReference type="GeneID" id="1441015"/>
    <dbReference type="KEGG" id="tvo:TVG0487282"/>
    <dbReference type="eggNOG" id="arCOG04804">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018614_3_0_2"/>
    <dbReference type="OrthoDB" id="56688at2157"/>
    <dbReference type="PhylomeDB" id="Q97BF7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008556">
        <property type="term" value="F:P-type potassium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00275">
        <property type="entry name" value="KdpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004623">
        <property type="entry name" value="KdpA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00680">
        <property type="entry name" value="kdpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30607">
        <property type="entry name" value="POTASSIUM-TRANSPORTING ATPASE A CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30607:SF2">
        <property type="entry name" value="POTASSIUM-TRANSPORTING ATPASE POTASSIUM-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03814">
        <property type="entry name" value="KdpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001294">
        <property type="entry name" value="K_ATPaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-0633">Potassium transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000166543" description="Potassium-transporting ATPase potassium-binding subunit">
        <location>
            <begin position="1"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="547"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00275"/>
        </source>
    </evidence>
    <sequence length="585" mass="64701" checksum="0F552556A5D553AD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BG0</accession>
    <name>Q97BG0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0480705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916754.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BG0"/>
    <dbReference type="SMR" id="Q97BG0"/>
    <dbReference type="STRING" id="273116.gene:9381278"/>
    <dbReference type="PaxDb" id="273116-14324711"/>
    <dbReference type="DNASU" id="1441012"/>
    <dbReference type="GeneID" id="1441012"/>
    <dbReference type="KEGG" id="tvo:TVG0480705"/>
    <dbReference type="eggNOG" id="arCOG04334">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_4_2"/>
    <dbReference type="OrthoDB" id="33635at2157"/>
    <dbReference type="PhylomeDB" id="Q97BG0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510:SF1">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="1">
        <location>
            <begin position="43"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="345" mass="39207" checksum="7CD190A6E296F386" modified="2001-10-01" version="1">MQWLTFQWLEFIREYREKAKHNLSNSGMPEPDLKSLGIDTSYEHYLDAAGEVEYRFTEALSRRLRVDQDLIIPTVGGTEAIFIALSHLSSVSKRIHVPLPEYEPMFLVPESLGIETSKIESKVMPGRISKNESIAMTLPNNPTAQYSDRISLLDNVIHSEGLIYIDETFRDFLPSGTRPTLFDGSETMVVSGTMTKFYGLSNLRTGWIISSEHNVAGMRRIKDLTSINNASFSLYIAAQAIQKWNYFKEAEDNLINKNLRVAREYLEGLPGISYKEPEGAPFIFLSYSKNIESVKLAKLAVERYGILFAPGSFFGEEHHIRVCLTSNKPEEDFNVLKEFLKKEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BI7</accession>
    <name>Q97BI7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0457223</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760311.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BI7"/>
    <dbReference type="STRING" id="273116.gene:9381250"/>
    <dbReference type="PaxDb" id="273116-14324683"/>
    <dbReference type="GeneID" id="1440986"/>
    <dbReference type="KEGG" id="tvo:TVG0457223"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021639_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BI7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="3">
        <location>
            <begin position="39"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="122" mass="13451" checksum="6D78CEBA728EF96F" modified="2001-10-01" version="1">MVSQRRFSRGRSPYSYVMRFDIMEFNLSMSNQKIEARDYDAVIIGSGAPGMSAAVYTARYGLKVVILGQSVAGGLTAEAPLVENYLGYKAIKGTDLASNFAKHAQEYSQIIDNTLRNIFHCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BJ4</accession>
    <name>Q97BJ4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mut7-C RNAse domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0450734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916720.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BJ4"/>
    <dbReference type="STRING" id="273116.gene:9381242"/>
    <dbReference type="PaxDb" id="273116-14324676"/>
    <dbReference type="GeneID" id="1440978"/>
    <dbReference type="KEGG" id="tvo:TVG0450734"/>
    <dbReference type="eggNOG" id="arCOG04290">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112469_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BJ4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002782">
        <property type="entry name" value="Mut7-C_RNAse_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39081">
        <property type="entry name" value="MUT7-C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39081:SF1">
        <property type="entry name" value="MUT7-C RNASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01927">
        <property type="entry name" value="Mut7-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mut7-C RNAse" evidence="1">
        <location>
            <begin position="13"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="157" mass="18670" checksum="EEBA9F245589AE98" modified="2001-10-01" version="1">MLKTNSSRDGKIKLTVDQMLGKLARWIRLMGYDVYYPAGNVSDNEIIEVSKNEGRILITKDYELYSRYPLSIYELYSNIDDQLYDFVFHFRKRTEEEFKRCPVCNGKLVKVYASPHVLNHRDLYRCEFCGKLYWKGTHYKKIYRKLKSLEKKSGAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BK9</accession>
    <name>Q97BK9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alcohol dehydrogenase [ADH]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0436799</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916706.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BK9"/>
    <dbReference type="SMR" id="Q97BK9"/>
    <dbReference type="STRING" id="273116.gene:9381226"/>
    <dbReference type="PaxDb" id="273116-14324661"/>
    <dbReference type="GeneID" id="71201312"/>
    <dbReference type="KEGG" id="tvo:TVG0436799"/>
    <dbReference type="eggNOG" id="arCOG01459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_753598_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BK9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="4">
        <location>
            <begin position="24"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="367" mass="40573" checksum="4F0FD78958AAE2BA" modified="2001-10-01" version="1">MRTLVLTGIKKMEIEDNKLEDPQRGWVTLKVNYAGIRGSELSAFVGQNELRKSLSVMGHEFTGKVVKLGEDVDPKLLNKNVAVNPLVTCGKCRYCKTGNRQLCLERKLIGVDYPGAFAEYVNVPEYSCYEIKEIPEASLAEPLATALRGVLQAGPEIDDRALVIGAGTIGLLATKIFSSVGCRKVVSMDINDNRLEHVDILINNAGICPFEDFFKIDIDLFEKVWKVNVESHYFITQAVSKHMIEKGIKGRILIMSSISGRVGGEFQTHYTTTKSALNGFMHSVAIVLGRYGILVNSLEPGTITTDINKDDLSNEEKRKYMERRTVVGRLGDPEDMVAPALFLVSDDNTYVTGSELLADGGMFINLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BL8</accession>
    <name>Q97BL8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">mRNA 3'-end processing factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0425663</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916695.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BL8"/>
    <dbReference type="STRING" id="273116.gene:9381216"/>
    <dbReference type="PaxDb" id="273116-14324652"/>
    <dbReference type="GeneID" id="1440953"/>
    <dbReference type="KEGG" id="tvo:TVG0425663"/>
    <dbReference type="eggNOG" id="arCOG00541">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="OrthoDB" id="40950at2157"/>
    <dbReference type="PhylomeDB" id="Q97BL8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF52">
        <property type="entry name" value="MRNA 3-END PROCESSING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="3">
        <location>
            <begin position="224"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="45212" checksum="5AA5F5DA778B94E4" modified="2001-10-01" version="1">MKLKFLGGAEEVGRLGVKITDRNTSVIVDYGVIPDKPPQYPMPPEPVDAMFITHSHLDHIGAIPVYYHKGEPDLYATQMTLNCMKPLLNDSLKVTNLEGYPAMFNEDDINTALANMKPARYFESFDVGDFKVTPYPAGHIPGSAMWKFEDGKSITVTGDVNTIDTYLINGAKPIKTDVLIIESTYAGKNHESREDVRKRFRESVREVIESGGKVVMPAFAVGRTQELIMTIADMGYDVSVDGMGNDISSIYLNTPGFLKSKRDFLRALGKVREVKGRRMRENAINSDIIISTSGMLDGGPVLGYIQKLMEDEKSAIFVTGYQVEGTNGRSLLENGTITVAGVTMKPKMRVEFFDMSAHAGHDELVSFIKAINPEKVVLMHGDHREELLPDLEGYDVLLPYNGKEYEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2018-07-18" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BM8</accession>
    <name>MBD_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mevalonate 3,5-bisphosphate decarboxylase</fullName>
            <shortName evidence="3">MBD</shortName>
            <ecNumber evidence="2">4.1.1.110</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Bisphosphomevalonate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">TV0427</name>
        <name evidence="6" type="ORF">TVG0411662</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="39737" last="39737">
            <title>An adaptation to life in acid through a novel mevalonate pathway.</title>
            <authorList>
                <person name="Vinokur J.M."/>
                <person name="Cummins M.C."/>
                <person name="Korman T.P."/>
                <person name="Bowie J.U."/>
            </authorList>
            <dbReference type="PubMed" id="28004831"/>
            <dbReference type="DOI" id="10.1038/srep39737"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the ATP-independent decarboxylation of (R)-mevalonate 3,5-bisphosphate to isopentenyl phosphate. Functions in an alternative mevalonate pathway, only present in extreme acidophiles of the Thermoplasmatales order, which passes through mevalonate 3-phosphate rather than mevalonate 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-3,5-bisphosphomevalonate + H(+) = isopentenyl phosphate + phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:56500"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:82774"/>
            <dbReference type="EC" id="4.1.1.110"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">It is proposed that Thermoplasmatales adapted the classical archaeal mevalonate pathway by replacing mevalonate 5-phosphate decarboxylase (MMD) with two specialized enzymes (mevalonate-3-phosphate 5-kinase and mevalonate 3,5-bisphosphate decarboxylase) in order to produce isoprenoids in extremely acidic environments. It was found that at low pH, the dual function enzyme MMD is unable to carry out the first phosphorylation step, yet retains its ability to perform decarboxylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the mevalonate 3,5-bisphosphate decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.110" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BM8"/>
    <dbReference type="SMR" id="Q97BM8"/>
    <dbReference type="STRING" id="273116.gene:9381205"/>
    <dbReference type="PaxDb" id="273116-14324642"/>
    <dbReference type="KEGG" id="tvo:TVG0411662"/>
    <dbReference type="eggNOG" id="arCOG02937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_743187_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BM8"/>
    <dbReference type="BRENDA" id="4.1.1.110">
        <property type="organism ID" value="6326"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00057"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053464">
        <property type="entry name" value="MVD_Decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040847">
        <property type="entry name" value="mev_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000444884" description="Mevalonate 3,5-bisphosphate decarboxylase">
        <location>
            <begin position="1"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9HJS1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="28004831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="28004831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="28004831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="372" mass="42524" checksum="CA9965D98E7BE7D7" modified="2001-10-01" version="1">MNIMEISKFQSLGDKIRTMLEDHGYLSENNDYEPNPIDGNISISYAYPIKAFEKFLGYYDVENRVAYNPSISMRTDFSYCIAACRYNKNGNEDTVILDGVTDEKYLRKAKFALDYFRKEFRIKGSFDFYIRRYRRYTKAKGLSESSAVAAAVSRALIKNVFGEGPALDDVFVSKYARLVSGSGTRAAHSGISIWLSYPGINLRECAAFRVADDPHDVYYGIFPKYTDIATDSAHSVAVKSIFYASWLEDKYANIKRLIEHNFDIDELLISGENDMLKLNAILFSGGLIIQTGESLRILRAIQDFKKNGDLFFTADTGPSIMVLSRDKSLIEELRQSVEDPYIEGTYNFNRHTRDLNNFTKEANEYFLENKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BR7</accession>
    <name>Q97BR7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0374366 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0374366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
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    <sequence length="102" mass="10708" checksum="5AE9383F891AFC92" modified="2001-10-01" version="1">MVEVTGVVLLIVALIDGLLFGLAIKKGVTSFILIIIALIISTYVGFSFAPHVSLTNVTAKLVPYIKTYADQITKAISIGFGGSLSLTIVLFLIGLGVGLWKG</sequence>
</entry>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
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                <person name="Suzuki M."/>
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        </source>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Makino S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
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            <end position="23"/>
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    <accession>Q97BU2</accession>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
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                <person name="Yamamoto Y."/>
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                <person name="Watanabe K."/>
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                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
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                <person name="Nunoshiba T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
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    </reference>
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    </evidence>
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        <source>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
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                <person name="Suzuki M."/>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916615.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BU4"/>
    <dbReference type="STRING" id="273116.gene:9381138"/>
    <dbReference type="PaxDb" id="273116-14324576"/>
    <dbReference type="DNASU" id="1440873"/>
    <dbReference type="GeneID" id="1440873"/>
    <dbReference type="KEGG" id="tvo:TVG0351553"/>
    <dbReference type="eggNOG" id="arCOG03016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1003336_0_0_2"/>
    <dbReference type="OrthoDB" id="213145at2157"/>
    <dbReference type="PhylomeDB" id="Q97BU4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="277" mass="31251" checksum="C97768CB9B292236" modified="2001-10-01" version="1">MKAIVFGGSGFIGSHIVNSLEADSVAYYSLEKGTNVKRNDAVWIQGDVTDYSKVEASMAGYDTVFFAVEDWAADEARNREILLNGIKNVVSAIKKASTDQKLVSFSMINQPSYPLEYFRTKRLVEDNTRVLKNGLVVRLSIVFGDGDHFTDRFVKLAEQVSHLPEEGNLAPVYVGDVVTVVNSIIKREGVYDICSNDNLPLIRIINYIRKNIGKKEIGSVKLEKGLSMLTETGLFSRYEAELMFQDYYRETSILDRYVKNPTSYLDFLRSILVEHTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BV8</accession>
    <name>Q97BV8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein eL19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl19e</name>
        <name evidence="8" type="ORF">TVG0341558</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916601.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BV8"/>
    <dbReference type="STRING" id="273116.gene:9381124"/>
    <dbReference type="PaxDb" id="273116-14324562"/>
    <dbReference type="GeneID" id="1440859"/>
    <dbReference type="KEGG" id="tvo:TVG0341558"/>
    <dbReference type="eggNOG" id="arCOG04089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083919_1_1_2"/>
    <dbReference type="OrthoDB" id="11624at2157"/>
    <dbReference type="PhylomeDB" id="Q97BV8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01418">
        <property type="entry name" value="Ribosomal_L19e_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033936">
        <property type="entry name" value="Ribosomal_eL19_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="7">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="149" mass="17186" checksum="F018FB3E1F852B7B" modified="2001-10-01" version="1">MRAETVKRIVSDLKGVGTSRVYIDTNKLDKIDEAATRSDILALIEQDVVKIKQKKGISNGRLKKRIKQLSKDRRRGPGSLRGTRNARYKRKERWIDTIRALRDELRKLKKDGKIDPAVYRKYYRIIKSGSIKSRAQLVSHIKSAGLLKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BW1</accession>
    <name>RS8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps8</name>
        <name type="ordered locus">TV0344</name>
        <name type="ORF">TVG0340161</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916598.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BW1"/>
    <dbReference type="SMR" id="Q97BW1"/>
    <dbReference type="STRING" id="273116.gene:9381121"/>
    <dbReference type="PaxDb" id="273116-14324559"/>
    <dbReference type="GeneID" id="1440856"/>
    <dbReference type="KEGG" id="tvo:TVG0340161"/>
    <dbReference type="eggNOG" id="arCOG04091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_2"/>
    <dbReference type="OrthoDB" id="5670at2157"/>
    <dbReference type="PhylomeDB" id="Q97BW1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000126556" description="Small ribosomal subunit protein uS8">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="129" mass="14539" checksum="CB9DCD960860F352" modified="2001-10-01" version="1">MRNDTLNDVINSIKNASRLGRREIIAEPAAKLIGKVLKVMQDYNYIKSFEVIDESRGGKFKIVLNTTINNCGVIKPRFPVKNENLEKYESRYLPAEDFGILILTTTKGVMSNIEARKLGIGGKLLAYVY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-07-19" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BW3</accession>
    <name>RL5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl5</name>
        <name type="ordered locus">TV0342</name>
        <name type="ORF">TVG0339596</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. May contact the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; contacts the 5S rRNA and probably tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916596.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BW3"/>
    <dbReference type="SMR" id="Q97BW3"/>
    <dbReference type="STRING" id="273116.gene:9381119"/>
    <dbReference type="PaxDb" id="273116-14324557"/>
    <dbReference type="GeneID" id="1440854"/>
    <dbReference type="KEGG" id="tvo:TVG0339596"/>
    <dbReference type="eggNOG" id="arCOG04092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_3_0_2"/>
    <dbReference type="OrthoDB" id="372044at2157"/>
    <dbReference type="PhylomeDB" id="Q97BW3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_A">
        <property type="entry name" value="Ribosomal_uL5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022804">
        <property type="entry name" value="Ribosomal_uL5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125072" description="Large ribosomal subunit protein uL5">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="170" mass="19385" checksum="92BF8AF87E266483" modified="2001-10-01" version="1">MNQMQEIIIDKVVVNIGVGQAGDRLTKAAKVLEMLTGHKPTQTLAKKSVRDFNIRKRLPIGVKVTLRKDDAVNFLNKALYVKDYKIPDYSFDKHGNAYFGISDYTDFKGMKYDPDIGIFGMDVAIVLKRRGGYRIEKRKIGKKTIPSSIRIKKDEAVEFLEKNFKVSVVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BX0</accession>
    <name>RL29_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl29</name>
        <name type="ordered locus">TV0335</name>
        <name type="ORF">TVG0336649</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BX0"/>
    <dbReference type="SMR" id="Q97BX0"/>
    <dbReference type="STRING" id="273116.gene:9381112"/>
    <dbReference type="PaxDb" id="273116-14324550"/>
    <dbReference type="KEGG" id="tvo:TVG0336649"/>
    <dbReference type="eggNOG" id="arCOG00785">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158491_2_2_2"/>
    <dbReference type="OrthoDB" id="11736at2157"/>
    <dbReference type="PhylomeDB" id="Q97BX0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_uL29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000130527" description="Large ribosomal subunit protein uL29">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="66" mass="7685" checksum="9BC256EDE61BB7D1" modified="2001-10-01" version="1">MLELRAKQIRQMSKEERQQTLKNLKESLLHERALVSMGGSSPSPGKVRSIRRQIARLLTVEREEKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-01-10" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BX1</accession>
    <name>RS3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps3</name>
        <name type="ordered locus">TV0334</name>
        <name type="ORF">TVG0336522</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the lower part of the 30S subunit head.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916588.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BX1"/>
    <dbReference type="SMR" id="Q97BX1"/>
    <dbReference type="STRING" id="273116.gene:9381111"/>
    <dbReference type="PaxDb" id="273116-14324549"/>
    <dbReference type="GeneID" id="1440846"/>
    <dbReference type="KEGG" id="tvo:TVG0336522"/>
    <dbReference type="eggNOG" id="arCOG04097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_1_1_2"/>
    <dbReference type="OrthoDB" id="9126at2157"/>
    <dbReference type="PhylomeDB" id="Q97BX1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02411">
        <property type="entry name" value="KH-II_30S_S3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000001">
        <property type="entry name" value="30S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_A">
        <property type="entry name" value="Ribosomal_uS3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027488">
        <property type="entry name" value="Ribosomal_uS3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130265" description="Small ribosomal subunit protein uS3">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="1">
        <location>
            <begin position="16"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="225" mass="24801" checksum="45BDBBFB26F52899" modified="2001-10-01" version="1">MKERKFVNEAVKRLLVYEYLVKETENAGFGKMSMKRTPFGTNITLYVNRPGLVIGRRGSKVQQMTETLEKKYAIETPQIEVKDVKDPDLNPSVIAKKIALSLEKGWSYRKAGNTSLKRTIDAGARGVLIKISGKISGERARYQKFIYGNVKYSGEPGSKGMLVGFSTAKLKVGILGVTVKILNPEYKLPDVFSISNLAGGENVGTESETDKADEQGREAANTEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BY5</accession>
    <name>Q97BY5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0326443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BY5"/>
    <dbReference type="STRING" id="273116.gene:9381096"/>
    <dbReference type="PaxDb" id="273116-14324535"/>
    <dbReference type="KEGG" id="tvo:TVG0326443"/>
    <dbReference type="eggNOG" id="arCOG01055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1615395_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="86"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="164" mass="18584" checksum="83D91067BAEADE99" modified="2001-10-01" version="1">MFMCSPLENTLREIVNASVVSNYPPSTCVSVVCSQNGDKEKMLAKSLSIYFVSKIIYHKPDGSSEVIKNEEENINLRFSQSVVTGRKQKAKIIFDILSFIKTPECTGIKSIIYNCNLNYRTAIKIQREMESRGPKEKQQEGRNGKYILTSKGTDFLEAIQFITL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-03" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C05</accession>
    <name>GCSPA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable glycine dehydrogenase (decarboxylating) subunit 1</fullName>
            <ecNumber evidence="1">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycine cleavage system P-protein subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine decarboxylase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine dehydrogenase (aminomethyl-transferring) subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvPA</name>
        <name type="ordered locus">TV0300</name>
        <name type="ORF">TVG0309943</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvP family. N-terminal subunit subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916555.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C05"/>
    <dbReference type="SMR" id="Q97C05"/>
    <dbReference type="STRING" id="273116.gene:9381074"/>
    <dbReference type="PaxDb" id="273116-14324515"/>
    <dbReference type="GeneID" id="1440813"/>
    <dbReference type="KEGG" id="tvo:TVG0309943"/>
    <dbReference type="eggNOG" id="arCOG00077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_0_2_2"/>
    <dbReference type="OrthoDB" id="17655at2157"/>
    <dbReference type="PhylomeDB" id="Q97C05"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00712">
        <property type="entry name" value="GcvPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023010">
        <property type="entry name" value="GcvPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001696">
        <property type="entry name" value="PRK00451.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42806">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM P-PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42806:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006815">
        <property type="entry name" value="GcvPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000166992" description="Probable glycine dehydrogenase (decarboxylating) subunit 1">
        <location>
            <begin position="1"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00712"/>
        </source>
    </evidence>
    <sequence length="434" mass="47950" checksum="E129009F955B5463" modified="2001-10-01" version="1">MDEISSMLDYLGIKSTEELFSDIPLSVRKKEIGIGSPLDEHLVLERARKYASLNSTEMLNFLGNGIYDRVIPEAVNYIISKSEFLDSYTPYQPEVSQGMLQSIFEYQSLISDLFKMDFTNASMYDGYSALGEAARMAYRINGKNKILIPESTYDSKLSVLKNYVWGLSMKIEKYKMNEEGKIDIDDLQSRIDGDTSAVVVENPNGYGVIDENVFRISEIKKESLLISYVDPISLGVLKPPGEYGSDIAIAEGQQLGIPMNFGGPLLGIMSFKADYVRKSPGRLIGESVDADGKRAFVMTLQTREQHIRRAKATSNICSNQALLTLAAGSYLSILGSSGLKKVALLTIKHSKNLAESLGNIGIEKVFDSISFSDTLFRIDKNVMLDLAKKGILGGIKLTSLIKDSKYSSGTFFTATEKTDNEKIKALVNALEVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C10</accession>
    <name>Q97C10_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator [Fur family]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0306138</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C10"/>
    <dbReference type="STRING" id="273116.gene:9381069"/>
    <dbReference type="PaxDb" id="273116-14324510"/>
    <dbReference type="KEGG" id="tvo:TVG0306138"/>
    <dbReference type="eggNOG" id="arCOG01868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_4_2_2"/>
    <dbReference type="PhylomeDB" id="Q97C10"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900376">
        <property type="term" value="P:regulation of secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202:SF7">
        <property type="entry name" value="FERRIC UPTAKE REGULATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="ZINC UPTAKE REGULATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="151" mass="17120" checksum="67A297C8DDF30CBE" modified="2001-10-01" version="1">MISMAKDYVSMLKDAGYKITPQRLAVIEYLKKGPGHFTANDVYKEIRKSIPTITLATVYNILKAFADVRCINSFDVDGTTWFENEVELHANLMCKVCGNIVDIKIDKEKLIKLLGENADADFENITLFVRGVCPSCKARQEEDMRSLQVHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C46</accession>
    <name>Q97C46_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0271512 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0271512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916516.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C46"/>
    <dbReference type="STRING" id="273116.gene:9381032"/>
    <dbReference type="PaxDb" id="273116-14324473"/>
    <dbReference type="GeneID" id="1440774"/>
    <dbReference type="KEGG" id="tvo:TVG0271512"/>
    <dbReference type="eggNOG" id="arCOG01378">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039939_1_0_2"/>
    <dbReference type="OrthoDB" id="33550at2157"/>
    <dbReference type="PhylomeDB" id="Q97C46"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="423" mass="48450" checksum="C0CF45C35F9D648E" modified="2001-10-01" version="1">MIIETYDKASEERIDSNRIKPNFKGYNLVNVPNSILDLFGIKNNRVLSEDIYTYIRGSYDKIVFFYLDGLGYDTFASDKFKNGIFKQIINNGYYAAITTVFPSTTAAANTTINTGLAPLEHGLLEWILYFDEARTVALTLPYRPITQRYRRRFNSMNPSYLFKGKTIFRKMKEDGVRSVSFLKANIVNGEYTKLIQDGSDVVGYSYTTDGLLLLKNYLLNGAYDYYYFYLDNIDHIGHVYGPSSEMYALEVQTTSKMIEETFEQLPMEVRRRTLVIITSDHGHIDVDPLRTIYLNSFRKLYDSLKSDGRYKIPPVGSPRDVFLFVKNDALESTIDYTKRKLASSLVMSTQESFDYGLFGGGAVPDSFYRRAGNMLILPSGNDLIWYRMTGLRDSRMKGMHGGLSEREMMIPIAFGEISQIIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C67</accession>
    <name>Q97C67_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0246963 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0246963</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916491.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C67"/>
    <dbReference type="STRING" id="273116.gene:9381008"/>
    <dbReference type="PaxDb" id="273116-14324451"/>
    <dbReference type="GeneID" id="1440751"/>
    <dbReference type="KEGG" id="tvo:TVG0246963"/>
    <dbReference type="eggNOG" id="arCOG05360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2419886_0_0_2"/>
    <dbReference type="OrthoDB" id="40443at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="86" mass="9653" checksum="A384B93E46611411" modified="2001-10-01" version="1">MVSIVVEHRWKEKNQDAAFKVVGSIIDMQKNGTLPAGFTLKSVNVIGSERMAVCEWDAPSLDDFKALLEKVNPPTKHEVFEAQRLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CD7</accession>
    <name>PUR5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="1">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purM</name>
        <name type="ordered locus">TV0165</name>
        <name type="ORF">TVG0175535</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916420.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CD7"/>
    <dbReference type="SMR" id="Q97CD7"/>
    <dbReference type="STRING" id="273116.gene:9380935"/>
    <dbReference type="PaxDb" id="273116-14324379"/>
    <dbReference type="GeneID" id="1441650"/>
    <dbReference type="KEGG" id="tvo:TVG0175535"/>
    <dbReference type="eggNOG" id="arCOG00639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_2"/>
    <dbReference type="OrthoDB" id="6605at2157"/>
    <dbReference type="PhylomeDB" id="Q97CD7"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148292" description="Phosphoribosylformylglycinamidine cyclo-ligase">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <sequence length="338" mass="37183" checksum="0AC488696971DEF5" modified="2001-10-01" version="1">MPEVKDGIIDRKMQGEFVSSFIRQLKFHREDFKNIGYIGGFTSLIDMGNFALSFNNDGVGTKTMIAEQANKYDTLGIDCVAMNVNDAITVGSEPIAMVDYLAVRDLNEDIAKQLGNGFNVGAQIANISIVGGETAVVPEIVNHIDVSGAVIGIVQKNQIITGANIKEGDVIIGLGSSGLHSNGFTTVRKIISDNEINLFDNFPGESKKTYEVLLEPTRIYVREILDVMGIIQIKGMANITGGGFKNITRMKDMKYVIDDPMEPQNVFVRLMELGNLNYKQMYEIFNMGTGFVVVIGEDDKIDFINTLKNRVPLKVIGHVENGTGVEIPKYSVSLMGYY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-05" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CD8</accession>
    <name>RS12_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps12</name>
        <name type="ordered locus">TV0164</name>
        <name type="ORF">TVG0173432</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916419.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CD8"/>
    <dbReference type="SMR" id="Q97CD8"/>
    <dbReference type="STRING" id="273116.gene:9380934"/>
    <dbReference type="PaxDb" id="273116-14324378"/>
    <dbReference type="GeneID" id="1441649"/>
    <dbReference type="KEGG" id="tvo:TVG0173432"/>
    <dbReference type="eggNOG" id="arCOG04255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115574_0_1_2"/>
    <dbReference type="OrthoDB" id="45154at2157"/>
    <dbReference type="PhylomeDB" id="Q97CD8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_A">
        <property type="entry name" value="Ribosomal_uS12_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022863">
        <property type="entry name" value="Ribosomal_uS12_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003254">
        <property type="entry name" value="PRK04211.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000146386" description="Small ribosomal subunit protein uS12">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="142" mass="15751" checksum="CA222E5A8B2C52BE" modified="2001-10-01" version="1">MGNGLNAGRKLLENRKKFRWSDRDYKRRVLQLKRKSDPLEGAPQAKGIAIEKVGIEAKQPNSAIRKCVKVQLIKNGRQITAFAVGDGAINYIDEHDEVTVEGIGGRMGRSKGDIPGVRYKVVAVNGISLKELVKGRKEKTVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-11-02" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CD9</accession>
    <name>RS7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps7</name>
        <name type="ordered locus">TV0163</name>
        <name type="ORF">TVG0172869</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CD9"/>
    <dbReference type="SMR" id="Q97CD9"/>
    <dbReference type="STRING" id="273116.gene:9380933"/>
    <dbReference type="PaxDb" id="273116-14324377"/>
    <dbReference type="KEGG" id="tvo:TVG0172869"/>
    <dbReference type="eggNOG" id="arCOG04254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063975_0_0_2"/>
    <dbReference type="OrthoDB" id="45346at2157"/>
    <dbReference type="PhylomeDB" id="Q97CD9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_A">
        <property type="entry name" value="Ribosomal_uS7_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026018">
        <property type="entry name" value="Ribosomal_uS7_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000124417" description="Small ribosomal subunit protein uS7">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="184" mass="20475" checksum="BFA9FCE6CE0082AE" modified="2001-10-01" version="1">MLFDKYDVNSVEVHDPGMVKYINVKSALNLHTGGRFSSYYAGKINMNVVERLINKLMRTEKWTGKKYSAYKITKDAFDIIADKTKQNPLQILINAIENAGPREEVTRLKYGGIAVPKSVDVSPSRRVDEALRNIATGATNASFKSKKSIVNCLADEIMLAAKNDASSFAISKKEEIERVAQSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CG5</accession>
    <name>Q97CG5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0143985 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0143985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916390.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CG5"/>
    <dbReference type="STRING" id="273116.gene:9380906"/>
    <dbReference type="PaxDb" id="273116-14324350"/>
    <dbReference type="GeneID" id="1441620"/>
    <dbReference type="KEGG" id="tvo:TVG0143985"/>
    <dbReference type="eggNOG" id="arCOG00724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521954_0_0_2"/>
    <dbReference type="OrthoDB" id="34535at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="176" mass="20631" checksum="D5077570358D4690" modified="2001-10-01" version="1">MVSRSDLLLSFIYAWGKSKKNNEPIPSVTHLQKEIFLLCRRTPFAEMKDVYHFEPLWYGPFSKELSGDLTDFQSRGVISFDEIRLTNKGFKIAASVWDHLTDFEKQQIFDVKSTFNYMSLTELLDTVYSRYPRFTKRSALKKEVVDKYFADFLQENKITEEDVVSAVNMAKKALRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CH9</accession>
    <name>Q97CH9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0128375 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0128375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916377.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CH9"/>
    <dbReference type="STRING" id="273116.gene:9380890"/>
    <dbReference type="PaxDb" id="273116-14324336"/>
    <dbReference type="GeneID" id="25392620"/>
    <dbReference type="KEGG" id="tvo:TVG0128375"/>
    <dbReference type="eggNOG" id="arCOG02761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010141_0_0_2"/>
    <dbReference type="OrthoDB" id="157520at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14797">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.70">
        <property type="entry name" value="TT1751-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005180">
        <property type="entry name" value="DUF302"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035923">
        <property type="entry name" value="TT1751-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342:SF2">
        <property type="entry name" value="INNER MEMBRANE OR EXPORTED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342">
        <property type="entry name" value="SLR5037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03625">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103247">
        <property type="entry name" value="TT1751-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF302" evidence="1">
        <location>
            <begin position="33"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="129" mass="14832" checksum="33D3C85288EEE44E" modified="2001-10-01" version="1">MDLKYESPVGYSETLSQLKNSIEKNGMKIVTEIDPQQNLKNAGISIEPQHIFEIFNPVYAKSVFDQNILAGIVPPLRIFVYVSEGKTWMMRESAIEKFRPYNIKDIPEKVEGIMEKIINDVIKNKVQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CK7</accession>
    <name>Q97CK7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0099129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916351.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CK7"/>
    <dbReference type="SMR" id="Q97CK7"/>
    <dbReference type="STRING" id="273116.gene:9380862"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324308"/>
    <dbReference type="GeneID" id="1441581"/>
    <dbReference type="KEGG" id="tvo:TVG0099129"/>
    <dbReference type="eggNOG" id="arCOG01398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055387_0_0_2"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="PhylomeDB" id="Q97CK7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="322" mass="37316" checksum="E0236CBCA2C0EAA0" modified="2001-10-01" version="1">MLLSVIITAQGRKGYVMQAVRSVLSQNIPKEDFEIIVSKDFKDDVIDEFLDGRAIVLNENHIRSGSRVASAVLQSSGKILVFLDDDDLFYPGKLREVKIVFSEMGVDYFKNSVKPVAESGDTVSYYQPAIKSDIFVKEPNEQDIRKLLKLRAGFNSSSISLKKDILVGYLDVLSKINLAVDSFYFFLYMSHSRPIYYSKKTLSLYRVLSKSTSRDVRNINAYAEFKSKFYELYSRDFETISEMASDGPCSKLAKTEFHLQMAYKWIFNTYRKEERPRMLHTLASSIYSHDPFIALPWPSFMKKIVQRREYFREKKLLSENTE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CP1</accession>
    <name>OGG1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">8-oxoguanine DNA glycosylase/AP lyase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1 3">8-oxoguanine DNA glycosylase</fullName>
                <shortName evidence="1">8-oxoG DNA glycosylase</shortName>
                <ecNumber evidence="1 2">3.2.2.-</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">DNA-(apurinic or apyrimidinic site) lyase</fullName>
                <shortName evidence="1 3">AP lyase</shortName>
                <ecNumber evidence="1 2">4.2.99.18</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">ogg</name>
        <name type="ordered locus">TV0060</name>
        <name type="ORF">TVG0063493</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2016" name="Archaea" volume="2016" first="8734894" last="8734894">
            <title>Characterization of a thermostable 8-oxoguanine DNA glycosylase specific for GO/N mismatches from the thermoacidophilic archaeon Thermoplasma volcanium.</title>
            <authorList>
                <person name="Fujii M."/>
                <person name="Hata C."/>
                <person name="Ukita M."/>
                <person name="Fukushima C."/>
                <person name="Matsuura C."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Tomobe K."/>
                <person name="Kawashima T."/>
            </authorList>
            <dbReference type="PubMed" id="27799846"/>
            <dbReference type="DOI" id="10.1155/2016/8734894"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the excision of an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine = 8-oxoG) from DNA. Also cleaves the DNA backbone at apurinic/apyrimidinic sites (AP sites). Prefers oligomers containing 8-oxoG:C, 8-oxoG:T and 8-oxoG:G base pairs, and is less effective on oligomers containing 8-oxoG:A mispairs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is about 7.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 60 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-2 OGG1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="1 2"/>
    <dbReference type="EC" id="4.2.99.18" evidence="1 2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916318.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CP1"/>
    <dbReference type="SMR" id="Q97CP1"/>
    <dbReference type="PaxDb" id="273116-14324274"/>
    <dbReference type="GeneID" id="1441548"/>
    <dbReference type="KEGG" id="tvo:TVG0063493"/>
    <dbReference type="eggNOG" id="arCOG04357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104937_0_0_2"/>
    <dbReference type="OrthoDB" id="35941at2157"/>
    <dbReference type="PhylomeDB" id="Q97CP1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00241">
        <property type="entry name" value="Ogg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012092">
        <property type="entry name" value="DNA_glyclase/AP_lyase_Ogg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002305">
        <property type="entry name" value="PRK01229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22175">
        <property type="entry name" value="Ogg-HhH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005954">
        <property type="entry name" value="Thrmst_ogg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159569" description="8-oxoguanine DNA glycosylase/AP lyase">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="site" description="Important for guanine/8-oxoguanine distinction" evidence="1">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="27799846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="27799846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="204" mass="24267" checksum="D5CF15B12D55E49D" modified="2001-10-01" version="1">MDFNQYFLPLFQGEASLIINKKVEEFKLFDRKNKDALFEELCFCILAANTSARMALDMQNRIGKGFLYLSENELREKLKAFKYRFYNVRPRYIVESRDIVDELPYIVSMDNKEEARDLLVENVYGFGYKEASHFLRNVGVFDFAILDKHILEWLSRYFQVKKNTSRKNYLYNESIFREIAKSVGMEPGVLDLYIWYMETGTVIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-08" modified="2026-01-28" version="145" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CQ0</accession>
    <name>AATA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">A-type ATP synthase subunit A</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Tvo AtpA intein</fullName>
            </recommendedName>
            <alternativeName>
                <fullName>Tvo VMA intein</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name evidence="2" type="primary">atpA</name>
        <name type="ordered locus">TV0051</name>
        <name type="ORF">TVG0054274</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The A chain is the catalytic subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="3">This protein undergoes a protein self splicing that involves a post-translational excision of the VDE intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The intein interrupts the ATP-binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916308.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="4O1S">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=234-421"/>
    </dbReference>
    <dbReference type="PDBsum" id="4O1S"/>
    <dbReference type="AlphaFoldDB" id="Q97CQ0"/>
    <dbReference type="SMR" id="Q97CQ0"/>
    <dbReference type="STRING" id="273116.gene:9380816"/>
    <dbReference type="PaxDb" id="273116-14324265"/>
    <dbReference type="GeneID" id="1441538"/>
    <dbReference type="KEGG" id="tvo:TVG0054274"/>
    <dbReference type="eggNOG" id="arCOG00868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008162_1_1_2"/>
    <dbReference type="OrthoDB" id="115235at2157"/>
    <dbReference type="PhylomeDB" id="Q97CQ0"/>
    <dbReference type="EvolutionaryTrace" id="Q97CQ0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00081">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000002473" description="A-type ATP synthase subunit A, 1st part" evidence="1">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000002474" description="Tvo AtpA intein" evidence="1">
        <location>
            <begin position="236"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000002475" description="A-type ATP synthase subunit A, 2nd part" evidence="1">
        <location>
            <begin position="422"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="242"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="250"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="266"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="274"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="319"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="327"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="335"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="341"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="349"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="355"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="405"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="410"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="417"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="4O1S"/>
        </source>
    </evidence>
    <sequence length="776" mass="86528" checksum="50FBF72E1F5D5C50" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CR4</accession>
    <name>Y037_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0215 protein TV0037</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0037</name>
        <name type="ORF">TVG0042157</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0215 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59179" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59179.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CR4"/>
    <dbReference type="SMR" id="Q97CR4"/>
    <dbReference type="STRING" id="273116.gene:9380802"/>
    <dbReference type="PaxDb" id="273116-14324251"/>
    <dbReference type="KEGG" id="tvo:TVG0042157"/>
    <dbReference type="eggNOG" id="arCOG00928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095956_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CR4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00582">
        <property type="entry name" value="UPF0215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002802">
        <property type="entry name" value="Endo_dU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518:SF2">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01949">
        <property type="entry name" value="Endo_dU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006380">
        <property type="entry name" value="UCP006380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149247" description="UPF0215 protein TV0037">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="194" mass="21243" checksum="A9DEEE7D2BBA39E1" modified="2002-10-25" version="2">MIPAIIGTRPNNSIALHKSGIRLLGVDDSPFRRGQQKSFIVGVIMRLDGYIEKVMKAQITIDGNDVTERISNMALKFKDIVRVIVLSGISFGGFNICDIELLFKLTGIPVISLFEKGGSASEMINAINKHLGDQEKIGILKRLKPIALNNNGYTIMANLAGIESESASRIIRMNTVRGKMPEAVRVPDLIAKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CR7</accession>
    <name>Q97CR7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0038039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916291.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CR7"/>
    <dbReference type="STRING" id="273116.gene:9380799"/>
    <dbReference type="PaxDb" id="273116-14324248"/>
    <dbReference type="GeneID" id="1441520"/>
    <dbReference type="KEGG" id="tvo:TVG0038039"/>
    <dbReference type="eggNOG" id="arCOG01843">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173013_0_0_2"/>
    <dbReference type="OrthoDB" id="56072at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="16"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="109" mass="12620" checksum="BEECFD005AEC266D" modified="2001-10-01" version="1">MKDTLQQLVLKVNKKFQDDPKYREKLKDVKKSVNLEFDGKDNYHFYLENAHLSDVEEGKIDADVNVMVSSEVFSKILSKEIDPLTAYLTKQIKIKASLMDKLLISDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CT3</accession>
    <name>Q97CT3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal-peptide peptidase, presenilin aspartyl protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0016337</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760287.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CT3"/>
    <dbReference type="STRING" id="273116.gene:9380783"/>
    <dbReference type="MEROPS" id="A22.015"/>
    <dbReference type="PaxDb" id="273116-14324232"/>
    <dbReference type="GeneID" id="1441503"/>
    <dbReference type="KEGG" id="tvo:TVG0016337"/>
    <dbReference type="eggNOG" id="arCOG04463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053464_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CT3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010545">
        <property type="entry name" value="SPP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041679">
        <property type="entry name" value="IMP_arch_presen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06550">
        <property type="entry name" value="SPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="32085" checksum="8F27D524EB0BCD83" modified="2001-10-01" version="1">MRAKAEITSMLIFVAASIMALLLAVAVYNSEPSISHRSPTYGLSYIFVYIVAAFLFSFVIIYLGRRKKLRIFRIIYSVVVAYVIFYVFLILLSIFLDNLYAILAISVAIAISYFYLLLFKNEWYITDTAGFFLAVGIASIWGITFGVWAAVAFLVVFEIYDYIAVYKTKHMISLARYAVDSDLPMLFVIPENRDFTMKNVTFENRGEGNALMIGFGDIALPSILVVSSALYGISNILYFTLLPLGGGLIGMAVLYFCNRDRPAPGLPYINTGTIAGFLIAFVMFKVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>P58306</accession>
    <name>PCKG_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoenolpyruvate carboxykinase [GTP]</fullName>
            <shortName evidence="1">PEP carboxykinase</shortName>
            <shortName evidence="1">PEPCK</shortName>
            <ecNumber evidence="1">4.1.1.32</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pckG</name>
        <name type="ordered locus">TV0200</name>
        <name type="ORF">TVG0204657</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2</text>
            <dbReference type="Rhea" id="RHEA:10388"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.32" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916456.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58306"/>
    <dbReference type="SMR" id="P58306"/>
    <dbReference type="STRING" id="273116.gene:9380970"/>
    <dbReference type="PaxDb" id="273116-14324414"/>
    <dbReference type="GeneID" id="1441686"/>
    <dbReference type="KEGG" id="tvo:TVG0204657"/>
    <dbReference type="eggNOG" id="arCOG05865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028872_1_1_2"/>
    <dbReference type="OrthoDB" id="55875at2157"/>
    <dbReference type="PhylomeDB" id="P58306"/>
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    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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        <location>
            <position position="236"/>
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            <name>Mn(2+)</name>
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    <feature type="binding site" evidence="1">
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        <location>
            <position position="287"/>
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    <feature type="binding site" evidence="1">
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    </evidence>
    <sequence length="589" mass="66908" checksum="E339F1A18012962B" modified="2001-09-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q978A0</accession>
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            <taxon>Thermoplasmata</taxon>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <location>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
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    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAB60659.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
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    </evidence>
    <sequence length="513" mass="58987" checksum="6A88CF627224A2F4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>Q978A7</accession>
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        <submittedName>
            <fullName evidence="1">TVG1564065 protein</fullName>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="273116.gene:9382324"/>
    <dbReference type="PaxDb" id="273116-14325749"/>
    <dbReference type="KEGG" id="tvo:TVG1564065"/>
    <dbReference type="HOGENOM" id="CLU_2152757_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="111" mass="13165" checksum="7E628151492A0676" modified="2001-10-01" version="1">MNGSEPEYFEGSSPEMVRYEMANPQKKGTLERLQETIIENTVNQYELEDRRQIAAIREFMERNPDISYVEYESNPVMRMDLVITRRGLIFKREEASISATLTPNRRFRAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q978B7</accession>
    <name>Q978B7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG1550926 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1550926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978B7"/>
    <dbReference type="PaxDb" id="273116-14325739"/>
    <dbReference type="KEGG" id="tvo:TVG1550926"/>
    <dbReference type="eggNOG" id="arCOG07527">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1187839_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031033">
        <property type="entry name" value="Halocin_C8_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04449">
        <property type="entry name" value="halocin_C8_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saposin B-type" evidence="2">
        <location>
            <begin position="146"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="233" mass="25289" checksum="65DD089C9D12B531" modified="2001-10-01" version="1">MDIMVCHKRLTAYKIGIYMYDDLIISLMAKKIKSVKVLHFDESTEEGARIQQASIFIEIEGEKPKLIQGTQVLKGDVNGNHTINYTIFDGKNIGKATYSINTMEKNKNDSKLKIVGISEGKACCGNSKPIDTTLVVSNKTYSSNDPSIQCDICQALVKEICEELADGIPSDEICADVCVAGAGDICLLFVETLIGYLICLSICASLCALAIEEITDYGCSVGAEYICQKVGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q978E1</accession>
    <name>Q978E1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1527611 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1527611</name>
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    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917707.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978E1"/>
    <dbReference type="PaxDb" id="273116-14325715"/>
    <dbReference type="GeneID" id="1442167"/>
    <dbReference type="KEGG" id="tvo:TVG1527611"/>
    <dbReference type="eggNOG" id="arCOG03911">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167328_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="118" mass="13905" checksum="B338C309D217B174" modified="2001-10-01" version="1">MDKKVENFAWLLIGIIALIAVVVFVYSIFAGGTFYSFGYGPRVIVGGLFGWWLIMPIFAFFVMIFFVIMMFSSHGHDHWHNNRMDFKRSRAEDIARERFAKGEITEDQYNRIINEIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q978F0</accession>
    <name>Q978F0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1516240 protein</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917698.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978F0"/>
    <dbReference type="STRING" id="273116.gene:9382279"/>
    <dbReference type="PaxDb" id="273116-14325706"/>
    <dbReference type="GeneID" id="1442158"/>
    <dbReference type="KEGG" id="tvo:TVG1516240"/>
    <dbReference type="eggNOG" id="arCOG05338">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_600799_0_0_2"/>
    <dbReference type="OrthoDB" id="56760at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="447" mass="50871" checksum="D3DF83FCDCCB223B" modified="2001-10-01" version="1">MKLKSISKEEQVKFKKKLEDFFSNPLSIIPQMLDSSLFCPITGYAKKLEKMRAKNDFSYSGSDGFLSAVSETFKLMSTDTPILGLIKTPYGTVHYGKRGNADEKLLAGIQNYDNDVWRMLAFSNVVLTKNVKIYSSKNYFLASCKNTPPPMEFFKDVLEEEGIKYSGQDEIEIGQEDPSFYMKLFGSITIIVHRESRYKTGYSILKHMLINDPYKEIRVGSTFLEECSQPDISGDVRRYLAGEYDDKYFLSKVIQERQSYIVKNGLYGIDQKCFDSIDSFISNIPGAMDYAPYLKRYSKGIYLEAPALGKLMDLLWPEIGEEISRDKAGMDFKSFQDMQSYLMRESRKGKVTFEPWSPDSAFLADLIKQSQEQGVNNAILQFKDMARTHVQKAILYAFNIIAGGPTIGYVFSDDDVVLGNEVKGIIELIVKGESVEENLKKLRAYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q978F6</accession>
    <name>Q978F6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyruvate dehydrogenase E3 / dihydrolipoamide dehydrogenase</fullName>
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    <organism evidence="2 3">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978F6"/>
    <dbReference type="STRING" id="273116.gene:9382271"/>
    <dbReference type="PaxDb" id="273116-14325698"/>
    <dbReference type="KEGG" id="tvo:TVG1508405"/>
    <dbReference type="eggNOG" id="arCOG01064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_5_0_2"/>
    <dbReference type="PhylomeDB" id="Q978F6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="381" mass="43093" checksum="A3168F584680E0A6" modified="2001-10-01" version="1">MMSMASKVLIVGDGDAGTIMANKLRFHTDIKDVEITVVGNSKEHYFKPDGVHISFSMIDYKRSVKPTEFLFNYGVNYVRDEVTRIDVADKTVSTKSGRNLDYDYLIIATGDRFTPEDIPGYETDAKHFYDLPHALELQKEIKSFKGGKIVIGQASIPIMCPPAPYEFTFLLEEYLNFHGLKDKTEIHYIYPLNRVFTIPNVSDFVAKRMEERGIITHTLFNVESVDPKNKKIQSLEGESINYDLLVLIPPHRGQKVITDSGLADDSGYIDVDKYKLNYKDYDNVFAIGDATNLPVSKAGATAHFQSMYLSNRIASETSGNIYNELYEGDVACTTVTGYEKGVTLYFNYNKPPKAKFDSKLDYFLKWQSADTFFSSMVRGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q978G0</accession>
    <name>Q978G0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MobA-like NTP transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1506684</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054042.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978G0"/>
    <dbReference type="STRING" id="273116.gene:9382267"/>
    <dbReference type="PaxDb" id="273116-14325694"/>
    <dbReference type="DNASU" id="1442144"/>
    <dbReference type="GeneID" id="1442144"/>
    <dbReference type="KEGG" id="tvo:TVG1506684"/>
    <dbReference type="eggNOG" id="arCOG01871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098907_1_0_2"/>
    <dbReference type="OrthoDB" id="9782at2157"/>
    <dbReference type="PhylomeDB" id="Q978G0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF86">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="163" mass="18001" checksum="D976323164471667" modified="2001-10-01" version="1">MKCLIMAGGLGTRFGEPEKALVKASGKPLIGRLIDEARNICDEIFVSIDPSMVNIKNFLGTVGVNIIAKNREGYVIDLNFSLNSINTYPILVLSADLYFKDPSILRKFTDMAMGRTEDIITFTLHGNPIGISLFKKPEGDFTNIDFDDGVVNVNTIDDLKKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q978M8</accession>
    <name>Q978M8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1433995 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1433995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917620.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978M8"/>
    <dbReference type="PaxDb" id="273116-14325626"/>
    <dbReference type="GeneID" id="1442078"/>
    <dbReference type="KEGG" id="tvo:TVG1433995"/>
    <dbReference type="eggNOG" id="arCOG07364">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2730624_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="71" mass="7901" checksum="BD527E76E7508B58" modified="2001-10-01" version="1">MAFKWTAKFIAGIISVVFIIIGGYLFGEVISKVIASINRVEFEWGLALLVIGIIILVVIIPKPKLSREKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Q2</accession>
    <name>Q978Q2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">2-(3-amino-3-carboxypropyl)histidine synthase</fullName>
            <ecNumber evidence="3 10">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG1411317</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DPH1/DPH2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 10"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Q2"/>
    <dbReference type="STRING" id="273116.gene:9382171"/>
    <dbReference type="PaxDb" id="273116-14325602"/>
    <dbReference type="KEGG" id="tvo:TVG1411317"/>
    <dbReference type="eggNOG" id="arCOG04112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037146_0_0_2"/>
    <dbReference type="OrthoDB" id="314at2157"/>
    <dbReference type="PhylomeDB" id="Q978Q2"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022428">
        <property type="entry name" value="Dph2_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03682">
        <property type="entry name" value="arCOG04112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB60505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="345" mass="39366" checksum="61D81C7F0112E2DE" modified="2001-10-01" version="1">MYKNNFENYMNLDIYKDIEEIRAALEHLGAKKILLQLPDGLKPEVYHYFNYLSANYNVVISSSPVYGACDIGPSSIYDKVDAIVQIGHSPMPNVKYPKPTIFVEHHYNARISNIDTSLLEGKYDRIGLLFSVQYKEVADEVRKIFENKGFTVIIGKKDGRMAYDGQVLGCNFSSVHSISYDVDAYVLVSTGQFHGIGAQLSTEREVFVLDLNDYKLHSMKDEADKFIRKRYAQIFRAKEARKICIISDTRIGQRRERLARIILRQVQQMGLEGILAVTDNISNQDLENMRCDAVVYTGCPRVPIDDQQKFTMPLLTPAEFEMGFVKNSNRYVLDEIVSVDDPADK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q978S3</accession>
    <name>AROA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="1">EPSP synthase</shortName>
            <shortName evidence="1">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroA</name>
        <name type="ordered locus">TV1342</name>
        <name type="ORF">TVG1387115</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EPSP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978S3"/>
    <dbReference type="SMR" id="Q978S3"/>
    <dbReference type="STRING" id="273116.gene:9382150"/>
    <dbReference type="PaxDb" id="273116-14325581"/>
    <dbReference type="KEGG" id="tvo:TVG1387115"/>
    <dbReference type="eggNOG" id="arCOG04134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024321_0_0_2"/>
    <dbReference type="OrthoDB" id="43788at2157"/>
    <dbReference type="PhylomeDB" id="Q978S3"/>
    <dbReference type="UniPathway" id="UPA00053"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000088340" description="3-phosphoshikimate 1-carboxyvinyltransferase">
        <location>
            <begin position="1"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <sequence length="410" mass="45187" checksum="11E334A6B255F077" modified="2001-10-01" version="1">MIVKVSNLGGSGIAEMPSSKSFTQRYVLASAFLNKSVVLNGITITNDDDVAMRIAESVGSTITINNRSIKISSNFKCPEEIYVGESGTSYRLSIGLLAASGCVTRIKGEDSLAKRPIEPLLMALGENGVKFERNEAGFYNVDGRNSQKKHVEIEGSSSQFVSSLMLYYAKKGGGEFTARNIKSIGYVYITKRVLYDLGYFANIERTITINPTGVWKTAIDVEPDYSSMAFFMVLGLLSDSVDVRFRIKRISRIQPDSVILDLFKNNILINGEEIRVISGINEPVSVDADMNPDLCPPLSVIGIFSKYGVQIRNYERLKTKESNRYEGIIDLAERFGANVEDNGQDLFIKPGSVRFPDVISYKDHRMIMAASIASLIGGFPTVIENAEKTAKSFPGFFAELSKFANVEELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q978V3</accession>
    <name>Q978V3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1353176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978V3"/>
    <dbReference type="STRING" id="273116.gene:9382120"/>
    <dbReference type="PaxDb" id="273116-14325551"/>
    <dbReference type="KEGG" id="tvo:TVG1353176"/>
    <dbReference type="eggNOG" id="arCOG05413">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184954_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058349">
        <property type="entry name" value="DUF8036"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26119">
        <property type="entry name" value="DUF8036"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="97" mass="10893" checksum="CA5520B5AECEB2D3" modified="2001-10-01" version="1">MKTMGIFWLTNIIIILIEAALLATIIAFYVKTIIAVKSKFLLPIVLFSTLFLLQSILSVYYYYNFSHFVGASVSVPLILINSIGLASFIVLFVSLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q978V8</accession>
    <name>Q978V8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">hydroxyacid-oxoacid transhydrogenase</fullName>
            <ecNumber evidence="3">1.1.99.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1348694</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-3-hydroxybutanoate + 2-oxoglutarate = (R)-2-hydroxyglutarate + acetoacetate</text>
            <dbReference type="Rhea" id="RHEA:23048"/>
            <dbReference type="ChEBI" id="CHEBI:11047"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="EC" id="1.1.99.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4-hydroxybutanoate + 2-oxoglutarate = (R)-2-hydroxyglutarate + succinate semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:24734"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16724"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="EC" id="1.1.99.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.24" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917542.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978V8"/>
    <dbReference type="STRING" id="273116.gene:9382114"/>
    <dbReference type="PaxDb" id="273116-14325546"/>
    <dbReference type="DNASU" id="1441424"/>
    <dbReference type="GeneID" id="1441424"/>
    <dbReference type="KEGG" id="tvo:TVG1348694"/>
    <dbReference type="eggNOG" id="arCOG00984">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007207_0_7_2"/>
    <dbReference type="OrthoDB" id="57329at2157"/>
    <dbReference type="PhylomeDB" id="Q978V8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047988">
        <property type="term" value="F:hydroxyacid-oxoacid transhydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08190">
        <property type="entry name" value="HOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000003">
        <property type="entry name" value="Alcohol dehydrogenase, iron-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042157">
        <property type="entry name" value="HOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF83">
        <property type="entry name" value="HYDROXYACID-OXOACID TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="7">
        <location>
            <begin position="22"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="8">
        <location>
            <begin position="241"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="433" mass="47533" checksum="922001E895A7704D" modified="2001-10-01" version="1">MEGNYLISTNFNDTAFVINVPKIKFGMNTTKEVGKDAVGLKIKNALVVVGPRLKDSKQFHDVLSSLSESGINYAVFDEVKTEPDDENLTKGYEAIKGKKIDGLISFGGGSTIDTAKILNLLYKYPRDINDYINKPIGKGMIPEGDLLPHIAIPTTAGTGSETTNVTIFDVERLHVKTGISNPFIRPSLAIIDPVNTLTMPPKVTASTGLDVLNHAIESYTSHPYTARPPVASPDERPVYSGSTPIGDLFGLQAIEWVHKYLRRAFADPLDLEARYYMTMGASIAGMGFGHVGVHIPHAMAYPIAGMIKKWHPDDYDFGYPISPHGISTAIPAAYVFRYLSKFVPERFADVLTALGYDTEGMSAEDIGDTLYGYYIDLLHALKIPDNLSDLDFTSSDIEKLVDGTMAQQRLIGLSPKKIDKSDIEFLFKEAMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Y4</accession>
    <name>Q978Y4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aliphatic amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1320838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917515.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Y4"/>
    <dbReference type="STRING" id="273116.gene:9382087"/>
    <dbReference type="PaxDb" id="273116-14325519"/>
    <dbReference type="GeneID" id="1441397"/>
    <dbReference type="KEGG" id="tvo:TVG1320838"/>
    <dbReference type="eggNOG" id="arCOG00062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_1_2_2"/>
    <dbReference type="OrthoDB" id="39312at2157"/>
    <dbReference type="PhylomeDB" id="Q978Y4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07581">
        <property type="entry name" value="nitrilase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="270" mass="30798" checksum="79270734BD3CD6E0" modified="2001-10-01" version="1">MRVTVVQMASTSDKSENIEKTFKFLDSIGNSNLIIFPEYQIFVPDFKNGSDMINIAESESGEFMSNIANYAKERKSKILVNIPEKNAFNLKPFNTAVLIDELGTTMKYRKLHLFDAFNFKESAFFEKGNMSPMVFNSSETPFGVQICYDLRFPEAARILTLYGAKILVYQAGWFSGERKYDQWKTLLRARAMENGVFVIGSAQTGDMFTGHSMVVSPYGDVVAELDKDENYISMDIDLGVVEKYRSEVPLLKERRTDIYDVLDVSLFEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Z3</accession>
    <name>Q978Z3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH marR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1311382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Z3"/>
    <dbReference type="STRING" id="273116.gene:9382074"/>
    <dbReference type="PaxDb" id="273116-14325508"/>
    <dbReference type="KEGG" id="tvo:TVG1311382"/>
    <dbReference type="eggNOG" id="arCOG03181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_4_2"/>
    <dbReference type="OrthoDB" id="10712at2157"/>
    <dbReference type="PhylomeDB" id="Q978Z3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039422">
        <property type="entry name" value="MarR/SlyA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33164:SF43">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR YETL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33164">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="154" mass="18144" checksum="1A1452305E9A94EA" modified="2001-10-01" version="1">MIEMAYDDDENSMAIKVWRLYVDLWKLWYKKNERNLNKIDLSITEFTIMRHLIENGPMSMAAIASLINVTPGWITGVVDKMEEKNLVTRNRDSTDRRIIKIAITDRGREVYENAKKNHYAFIRKALAELNENELQQTYVLLQKMMDDVSKLESN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q979B2</accession>
    <name>Y1250_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>UPF0179 protein TV1250</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV1250</name>
        <name type="ORF">TVG1288366</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0179 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917484.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979B2"/>
    <dbReference type="STRING" id="273116.gene:9382055"/>
    <dbReference type="PaxDb" id="273116-14325488"/>
    <dbReference type="GeneID" id="1441366"/>
    <dbReference type="KEGG" id="tvo:TVG1288366"/>
    <dbReference type="eggNOG" id="arCOG04477">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121764_0_0_2"/>
    <dbReference type="OrthoDB" id="24613at2157"/>
    <dbReference type="PhylomeDB" id="Q979B2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00498">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005369">
        <property type="entry name" value="UPF0179"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002253">
        <property type="entry name" value="PRK01177.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40699">
        <property type="entry name" value="UPF0179 PROTEIN MJ1627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40699:SF1">
        <property type="entry name" value="UPF0179 PROTEIN MJ1627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03684">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006595">
        <property type="entry name" value="UCP006595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156878" description="UPF0179 protein TV1250">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="145" mass="16548" checksum="0175D18BFAD3AFBC" modified="2001-10-01" version="1">MPKISLIGKDLAKEGIEFVFAGPLPTCSDCRVKNVCFNLEQGHKYRVTKVREQLNPCIIFNGDKVNTVEVEELEDFFIIQESKKLQEGAIVTMKSMNCDYITCPNIEKCNLYYQKNDLKVAIKSIGQNVNCPKGFKMKKVQVTYK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q979B9</accession>
    <name>GFCR_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcriptional regulator GfcR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gfcR</name>
        <name type="ordered locus">TV1242</name>
        <name type="ORF">TVG1281244</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="1">Contains an N-terminal DNA-binding winged helix-turn-helix domain and a C-terminal regulatory domain (or effector binding domain) resembling phosphoribosyltransferase (PRT) domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. GfcR subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917476.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979B9"/>
    <dbReference type="SMR" id="Q979B9"/>
    <dbReference type="STRING" id="273116.gene:9382047"/>
    <dbReference type="PaxDb" id="273116-14325480"/>
    <dbReference type="GeneID" id="1441358"/>
    <dbReference type="KEGG" id="tvo:TVG1281244"/>
    <dbReference type="eggNOG" id="arCOG00028">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111001_0_0_2"/>
    <dbReference type="OrthoDB" id="68893at2157"/>
    <dbReference type="PhylomeDB" id="Q979B9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006222">
        <property type="term" value="P:UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01214">
        <property type="entry name" value="GfcR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022854">
        <property type="entry name" value="GfcR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002620">
        <property type="entry name" value="PRK02277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF41">
        <property type="entry name" value="PYRE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000110814" description="Transcriptional regulator GfcR">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01214"/>
        </source>
    </evidence>
    <sequence length="198" mass="21923" checksum="91BB3C5850B6D235" modified="2001-10-01" version="1">MKSIEELYKRALELKNKGMSDKEISTELHLSVNTVTWLLSKEFLKEGAVQDVKIGWRSLGVFGSRIISIAEVMSDIIREEMQRNSFEVDSILGIAINGIPYATMVSYLMDKELIVYRPHPARKEGFFSSNFAGVEGKRIVIIDDVASTGETLKRTITDVTKEGGKAVLCVILASKMGVDDLNGVPVRSLLRTRIIGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q979C1</accession>
    <name>Q979C1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-terminal acetyltransferase complex subunit [ARD1]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1279984</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917474.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979C1"/>
    <dbReference type="STRING" id="273116.gene:9382045"/>
    <dbReference type="PaxDb" id="273116-14325478"/>
    <dbReference type="GeneID" id="1441356"/>
    <dbReference type="KEGG" id="tvo:TVG1279984"/>
    <dbReference type="eggNOG" id="arCOG00833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_0_2"/>
    <dbReference type="OrthoDB" id="43754at2157"/>
    <dbReference type="PhylomeDB" id="Q979C1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF8">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="150" mass="17571" checksum="3D295C42084CE4DB" modified="2001-10-01" version="1">MIVRRYLKQDLCAVARLEVRSFEIGPYDKEYLREVLENASSISYIAQISDKIVGYIVAMPLNSQEIDIESIATDPDFRKRSIGTRLIARIENESKSLGYRNIILEVRKENIEAISFYSRLGFNIKEFIVNYYEEYYRGSRDAYRMVKSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q979C8</accession>
    <name>Q979C8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1270423</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917468.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979C8"/>
    <dbReference type="STRING" id="273116.gene:9382038"/>
    <dbReference type="PaxDb" id="273116-14325471"/>
    <dbReference type="GeneID" id="1441350"/>
    <dbReference type="KEGG" id="tvo:TVG1270423"/>
    <dbReference type="eggNOG" id="arCOG03142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1292065_0_0_2"/>
    <dbReference type="OrthoDB" id="17710at2157"/>
    <dbReference type="PhylomeDB" id="Q979C8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="219" mass="24694" checksum="D946B7E6023D7304" modified="2001-10-01" version="1">MPRIGIITYDFKFYHDVIQYLRRWKLPFVSLENTYSIPDDVVVIISSKNDNIELPDQIKAENGLDGIRRAIPKLLNKSSFVDLVIGIDPGPKPGIAVLGDGILIEAFECPRVTEIKTYVESIASSYTYQNITLKIGNGDKPNRDIIITRVSEIDIPIEIVNEKNTSTPHKIHDNALSAARIALINGLYPIKRIPVKFSRKDVYEKEFTTLRSAIGNEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q979E9</accession>
    <name>Q979E9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1244633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917444.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979E9"/>
    <dbReference type="STRING" id="273116.gene:9382015"/>
    <dbReference type="PaxDb" id="273116-14325450"/>
    <dbReference type="GeneID" id="1441326"/>
    <dbReference type="KEGG" id="tvo:TVG1244633"/>
    <dbReference type="eggNOG" id="arCOG01063">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207666_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="148"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="232" mass="26699" checksum="03CE13071A465111" modified="2001-10-01" version="1">MSKEGEKNLEPIYNKSINFQGKFRIASNVRIKGLSGNEHFFDFAIINEDGTTIPIMIAKDTLDSALLKEFEKKSLDVQSRIKIVVLHYGPAILQELNELMWDGNIIVFIPNTLFNSYDDSLLSLDENRPIKENDSTRYKDNLSLLKKKRTRLAMVLEMLSYLKDAEGGLPLTRLFFRCNLNYKKGEEIVKGLLDSGMVKIKNGRRQKNVMITPLGENFLRNYSKLHSELHKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-15" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q979F5</accession>
    <name>RPO1C_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo1C</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit A''</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo1C</name>
        <name evidence="1" type="synonym">rpoA2</name>
        <name type="ordered locus">TV1206</name>
        <name type="ORF">TVG1233699</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms part of the jaw domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979F5"/>
    <dbReference type="SMR" id="Q979F5"/>
    <dbReference type="STRING" id="273116.gene:9382008"/>
    <dbReference type="PaxDb" id="273116-14325444"/>
    <dbReference type="KEGG" id="tvo:TVG1233699"/>
    <dbReference type="eggNOG" id="arCOG04256">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037097_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q979F5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06528">
        <property type="entry name" value="RNAP_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00411">
        <property type="entry name" value="RNApol_arch_Rpo1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012757">
        <property type="entry name" value="RPO1C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02389">
        <property type="entry name" value="RNA_pol_rpoA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF32">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000074030" description="DNA-directed RNA polymerase subunit Rpo1C">
        <location>
            <begin position="1"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Unknown">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="DNA-directed RNA polymerase subunit Rpo1C">
        <location>
            <begin position="124"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00411"/>
        </source>
    </evidence>
    <sequence length="508" mass="56950" checksum="0B17ABCBFE0118FE" modified="2001-10-01" version="1">MIIWKDTAKNMSLLSKSVPAKYAVDFEVPKGITEGYVTSDKKRFTYHVSISSVAPYSNESDVIQKKKSGLKSIMEIEKIQKIEPISIMEFRSSGKRIDELLTYAIAERETAEIREKYEYEKKVSSQVLDVIAEAKKLGYNIPESVAEEILRRKEEWGEKKYREILKRIGEEIQDELIDPYEAVGIIAAQSIGEPGTQMTMRTFHFAGVREMNVTLGLPRLIEIVDARRIPSTPSMTIYLKPEFETNDEVVMDVVKRLENTSVSDVADIITDIGELTITVRPDPNKMNDRLINQDDLVNAIYKVKGVTVMEESGQIIVKPQQESFKKLYLLQEQIKALPIKGISGIKRAIARVEGKEHRWVIYTQGSNLKDVLEVDEVDPTRTYTNDIVEIATVLGIEAARNAILNEAQRTLQEQGLNVDVRHLMLVADMMTFSGSVRAVGRTGISGRKSSVLARAAFEITTKHLLRAGIMGEVDKLAGVAENIIVGQPITLGTGAVDIIYKGYPKTKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-02" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q979H8</accession>
    <name>APGM_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="1">BPG-independent PGAM</shortName>
            <shortName evidence="1">Phosphoglyceromutase</shortName>
            <shortName evidence="1">aPGAM</shortName>
            <ecNumber evidence="1">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">apgM</name>
        <name type="ordered locus">TV1183</name>
        <name type="ORF">TVG1213330</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917416.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979H8"/>
    <dbReference type="SMR" id="Q979H8"/>
    <dbReference type="STRING" id="273116.gene:9381984"/>
    <dbReference type="PaxDb" id="273116-14325421"/>
    <dbReference type="GeneID" id="1441298"/>
    <dbReference type="KEGG" id="tvo:TVG1213330"/>
    <dbReference type="eggNOG" id="arCOG01696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034906_2_0_2"/>
    <dbReference type="OrthoDB" id="52918at2157"/>
    <dbReference type="PhylomeDB" id="Q979H8"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_A">
        <property type="entry name" value="ApgM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003104">
        <property type="entry name" value="PRK04024.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209:SF0">
        <property type="entry name" value="METALLOENZYME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138151" description="2,3-bisphosphoglycerate-independent phosphoglycerate mutase">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="406" mass="44101" checksum="AB648254355C835A" modified="2001-10-01" version="1">MIDVKSIILIVLDGLGDRPGEILGYRTPLQAAYHPNMNRMASLGMTGLMHPISPGIRSGSDTSHMSLLGYDPRVYYQGRGPFEALGLHMDMKPGDLAFRANFATNRDGKIIDRRAGRINAGNDQLASAISIDIGNYKFRVKSGVEHRAALVVSGPNLSDKISDSDPHSEGKPPEPIRPLDPSADSTAKIMNEYLKRIREILRDHPVNVEREKNGQIPGNELLIRSAGKVPDIPSFQEKNGITGACVVGSPWLKGLCRLLGMAVIDVPGAAGTINSNYTGKIKTAIDASKRYDFVLVNIKATDVAGHDGDYELKRRVIEDIDIAMEPLLGQSDRIVVAITGDHSTPCSVKDHSGDPVPIVFYTDGIYSDDVKLFDEVSAMKGALRITTQDVLNILMEMAGRAEKFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q979I6</accession>
    <name>Q979I6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Acetate--CoA ligase</fullName>
            <ecNumber evidence="2 6">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1206092</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917408.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979I6"/>
    <dbReference type="STRING" id="273116.gene:9381976"/>
    <dbReference type="PaxDb" id="273116-14325413"/>
    <dbReference type="GeneID" id="1441290"/>
    <dbReference type="KEGG" id="tvo:TVG1206092"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="PhylomeDB" id="Q979I6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043955">
        <property type="term" value="F:3-hydroxypropionyl-CoA synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043427">
        <property type="term" value="P:carbon fixation by 3-hydroxypropionate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="9">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="79"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="8">
        <location>
            <begin position="527"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR02188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="650" mass="73561" checksum="601D8E39BB9A914A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q979J6</accession>
    <name>Q979J6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1191381 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1191381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917398.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979J6"/>
    <dbReference type="STRING" id="273116.gene:9381966"/>
    <dbReference type="PaxDb" id="273116-14325403"/>
    <dbReference type="GeneID" id="1441280"/>
    <dbReference type="KEGG" id="tvo:TVG1191381"/>
    <dbReference type="eggNOG" id="arCOG07374">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1615397_0_0_2"/>
    <dbReference type="OrthoDB" id="55841at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="164" mass="18619" checksum="1CCC75D22CA656BD" modified="2001-10-01" version="1">MNKSAIRSFIRNISRYVYSDAQLTILGKSGYIRWTLRRVPGQVIFEDENGIDPDYSERIMDVVKTGGYASAILRSITSETEIKTGGSFMEIRGYFIYINAGVISWNGLSAEDVFDAVNHDFKTGRKLKPDAREIYCGLDGECLDYSKKSVVLVKRKIKAKKLHY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q979K5</accession>
    <name>COXX_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protoheme IX farnesyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heme B farnesyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ctaB</name>
        <name type="ordered locus">TV1156</name>
        <name type="ORF">TVG1185779</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>heme b + (2E,6E)-farnesyl diphosphate + H2O = Fe(II)-heme o + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917390.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979K5"/>
    <dbReference type="SMR" id="Q979K5"/>
    <dbReference type="STRING" id="273116.gene:9381956"/>
    <dbReference type="PaxDb" id="273116-14325394"/>
    <dbReference type="GeneID" id="1441272"/>
    <dbReference type="KEGG" id="tvo:TVG1185779"/>
    <dbReference type="eggNOG" id="arCOG00479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029631_0_1_2"/>
    <dbReference type="OrthoDB" id="131615at2157"/>
    <dbReference type="PhylomeDB" id="Q979K5"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003349">
        <property type="entry name" value="PRK04375.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000327201" description="Protoheme IX farnesyltransferase">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <sequence length="288" mass="32831" checksum="9CEBBDA9BF1CD6FE" modified="2001-10-01" version="1">MVSRVRLYFGYTKPKVWSLLVFVGVIGAIVAINRFTLTNILLILIATVSITLGSMGAEATTNYIDRDIDAIMDRTKKRPLVTGQIKPVKGLYFGLILMFLSIIILLFFSKYLAAVFMAIGLFDNVFIYSYLTKRRTPWNIILGGFSGGFPVVIGWYTVTNAFSVLPWFLFALVVIWIPIHVWSLAYRYRDDYNRARVPMMTSIQSDRISAICISSSAVILFIFSIIPVFFKAMPYTYMIVATIIAIPMLVYSVLFVRKPDRKSSLKLFIYSSPYLAIIFVLVLIFKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q979N8</accession>
    <name>Q979N8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1154372</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917356.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979N8"/>
    <dbReference type="PaxDb" id="273116-14325360"/>
    <dbReference type="GeneID" id="1441238"/>
    <dbReference type="KEGG" id="tvo:TVG1154372"/>
    <dbReference type="eggNOG" id="arCOG01178">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047518_0_0_2"/>
    <dbReference type="OrthoDB" id="49590at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046777">
        <property type="entry name" value="GvpD_bR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009788">
        <property type="entry name" value="GvpD_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF2">
        <property type="entry name" value="PROTEIN GVPD 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20440">
        <property type="entry name" value="GvpD_bR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07088">
        <property type="entry name" value="GvpD_P-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GvpD P-loop" evidence="3">
        <location>
            <begin position="10"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="GvpD basic region 2" evidence="4">
        <location>
            <begin position="247"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="53677" checksum="66C49036230446CD" modified="2001-10-01" version="1">METDPIMYAFERFFDQKFGKSLVIKGRPGSGKTTFTLDFLNAVRDHHPVYYISSRSSDASILETYPWIKNEYEIKGSVEKVKTSALRNFEKMVEEGKFGDALRDGLIVETSKIVPIIQALYEFVDNHFGESPIIALDSIDALAEEYDIPEDYLFLMLKNDLVEGSGAHLITILEARQNERLEYFADGVVTLDYNVKNDMLVRSLTLEKMRGISVGPSPTYMFSLADGRFRSVTRNTLVFPEKRIELDPGNDESMEFQVSMGDREYGKITAEGTDSIPGGSIVLLHVKDRSPAINDFINLFKTNLIINNIVHGRGVIDMTASGYETYTVLLKALGGKYLNNYITASKSDLINPYVISLKGERIKDDLNREIIEGKLAQSKGPYVYFFSSDYLFLVYGFNFLNQIVEVVDDIRTSGVIFFVADDDTYSKMFTASHLSLDLFSVNGYAAVSSNKTSSFLVQLVRDQNQWPKIKFVEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q979T9</accession>
    <name>Q979T9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1098940</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760263.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979T9"/>
    <dbReference type="STRING" id="273116.gene:9381867"/>
    <dbReference type="PaxDb" id="273116-14325309"/>
    <dbReference type="GeneID" id="71201325"/>
    <dbReference type="KEGG" id="tvo:TVG1098940"/>
    <dbReference type="eggNOG" id="arCOG02682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_10_2"/>
    <dbReference type="PhylomeDB" id="Q979T9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="MFS_dom"/>
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        <property type="entry name" value="MFS_sugar_transport-like"/>
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        <property type="entry name" value="MFS_trans_sf"/>
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    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF34">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00083">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
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    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="22"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="210" mass="23434" checksum="ADD02073F6D1B099" modified="2001-10-01" version="1">MDYKTGYVQFDQIRITPIFVAVTVLSSMGVFMDGFALSIFSAALPYLKNYILVKSILVSLAASAIYIGMFVGSFVIGHLSDTIGRKRMYTYDLAITSLFLVLTGISRNFAEFFIFELLAGIGIGADYPLSSSIEAEFSPRVSRGRFLVFNIFMWTIGSIVFYLISIPLVGYFGSSSWRWMYFVGAIIPIAVIISRHALPESPYWLVKAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q979U5</accession>
    <name>Q979U5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979U5"/>
    <dbReference type="STRING" id="273116.gene:9381860"/>
    <dbReference type="PaxDb" id="273116-14325303"/>
    <dbReference type="KEGG" id="tvo:TVG1089346"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_16_0_2"/>
    <dbReference type="PhylomeDB" id="Q979U5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
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            <end position="96"/>
        </location>
    </feature>
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            <end position="181"/>
        </location>
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            <end position="246"/>
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    </feature>
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            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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        </location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
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        <location>
            <begin position="534"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="578" mass="62629" checksum="6BA574E6F71102D9" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q979V4</accession>
    <name>Q979V4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tryptophan synthase</fullName>
            <ecNumber evidence="4">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1081798</name>
    </gene>
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        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917285.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979V4"/>
    <dbReference type="STRING" id="273116.gene:9381850"/>
    <dbReference type="PaxDb" id="273116-14325294"/>
    <dbReference type="GeneID" id="1441167"/>
    <dbReference type="KEGG" id="tvo:TVG1081798"/>
    <dbReference type="eggNOG" id="arCOG01086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_0_2_2"/>
    <dbReference type="OrthoDB" id="25658at2157"/>
    <dbReference type="PhylomeDB" id="Q979V4"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406:SF1">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE ALPHA CHAIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE, ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0822">Tryptophan biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="237" mass="26488" checksum="884755035B47D0CA" modified="2001-10-01" version="1">MKPFVYFTLGYKYNEVKKFIKNSDGVYAFEFGFPTENPLYDGKKIKATHSFAVNKFSHDDNAEIFKLAESMGIKKYALLYYSVFSGKKGILDYLSSSRFDGVMVPDVGIDYPDRIEQVAKAIADHGMDYVPFVTPVTPFKIMERQMKVSGEIVYQGMMPATGIQLPYDIGTIYGHIRDFTSGKRIVYGFGIRNKCTIAKIAKYGGFGIAVGTAVVEMLDDGNVKAYRQLINDILGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q979W2</accession>
    <name>Q979W2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glucose 1-dehydrogenase</fullName>
            <shortName evidence="10">GDH</shortName>
            <shortName evidence="10">GlcDH</shortName>
            <ecNumber evidence="10">1.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gdh</name>
        <name evidence="13" type="ORF">TVG1073292</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="15 16 17" key="3">
        <citation type="journal article" date="2014" name="Acta Crystallogr. D" volume="70" first="1271" last="1280">
            <title>Structural insight into glucose dehydrogenase from the thermoacidophilic archaeon Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kanoh Y."/>
                <person name="Uehara S."/>
                <person name="Iwata H."/>
                <person name="Yoneda K."/>
                <person name="Ohshima T."/>
                <person name="Sakuraba H."/>
            </authorList>
            <dbReference type="PubMed" id="24816096"/>
            <dbReference type="DOI" id="10.1107/s1399004714002363"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) IN COMPLEX WITH NADP(+) AND ZN(2+)</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Can utilize both NAD(+) and NADP(+) as electron acceptor. Is involved in the degradation of glucose through a non-phosphorylative variant of the Entner-Doudoroff pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-glucose + NAD(+) = D-glucono-1,5-lactone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14293"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-glucose + NADP(+) = D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14405"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the zinc-containing alcohol dehydrogenase family. Glucose 1-dehydrogenase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.47" evidence="10"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917277.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="3WIC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B/C/D=1-361"/>
    </dbReference>
    <dbReference type="PDB" id="3WID">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.25 A"/>
        <property type="chains" value="A/B/C/D=1-361"/>
    </dbReference>
    <dbReference type="PDB" id="3WIE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.33 A"/>
        <property type="chains" value="A/B/C/D=1-361"/>
    </dbReference>
    <dbReference type="PDBsum" id="3WIC"/>
    <dbReference type="PDBsum" id="3WID"/>
    <dbReference type="PDBsum" id="3WIE"/>
    <dbReference type="AlphaFoldDB" id="Q979W2"/>
    <dbReference type="SMR" id="Q979W2"/>
    <dbReference type="STRING" id="273116.gene:9381842"/>
    <dbReference type="PaxDb" id="273116-14325286"/>
    <dbReference type="GeneID" id="1441159"/>
    <dbReference type="KEGG" id="tvo:TVG1073292"/>
    <dbReference type="eggNOG" id="arCOG01459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_1_0_2"/>
    <dbReference type="OrthoDB" id="41394at2157"/>
    <dbReference type="PhylomeDB" id="Q979W2"/>
    <dbReference type="BRENDA" id="1.1.1.360">
        <property type="organism ID" value="6326"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q979W2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047910">
        <property type="term" value="F:galactose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005534">
        <property type="term" value="F:galactose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047934">
        <property type="term" value="F:glucose 1-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047935">
        <property type="term" value="F:glucose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033498">
        <property type="term" value="P:galactose catabolic process via D-galactonate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019595">
        <property type="term" value="P:non-phosphorylated glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08230">
        <property type="entry name" value="glucose_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000957">
        <property type="entry name" value="Glucose 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000074">
        <property type="entry name" value="Glucose 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02127">
        <property type="entry name" value="Glucose_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026583">
        <property type="entry name" value="Glc_1-DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031640">
        <property type="entry name" value="Glu_dehyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43189:SF2">
        <property type="entry name" value="GLUCOSE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43189">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C1198.01-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16912">
        <property type="entry name" value="Glu_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="15 16 17" id="KW-0002">3D-structure</keyword>
    <keyword evidence="9 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 10 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0520">NAD</keyword>
    <keyword evidence="6 10" id="KW-0521">NADP</keyword>
    <keyword evidence="4 10 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="11">
        <location>
            <begin position="30"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="150"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 16 17">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 16 17">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 16 17">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 16 17">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="216"/>
            <end position="218"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="275"/>
            <end position="277"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="304"/>
            <end position="306"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAB60190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="15">
        <source>
            <dbReference type="PDB" id="3WIC"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="16">
        <source>
            <dbReference type="PDB" id="3WID"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="17">
        <source>
            <dbReference type="PDB" id="3WIE"/>
        </source>
    </evidence>
    <sequence length="361" mass="40246" checksum="791DA4866355F377" modified="2001-10-01" version="1">MSTINAIVTDAPKGGVKYTKIDMPEPEKYEAKLKPVYIGICGTDRGEVAGALSFTYNPEGENFLVLGHEALLQVLDVSDNNYIKRGDFVVPLVRRPGKCVNCRIGRQDNCSIGDPDKHEAGITGLHGFMRDVIYDDIQNLVKVNDPDLGKIAVLTEPLKNVMKAFEVFDVVSKRSIFQNDDSTFIGKKMVVIGSGSEAFLYSFVGKDRGFDVTMVNRHDETENKMKMMDDFGVGFSNYLKDMPDKIDLLVDTSGDPSTIFKFVKKVNNNGVVILFGTNGKAPGYPVNGEDIDYIVERNITIAGSVDAAKIHYVQALDSLSNWYHRHPQTIKDIITYEAKPEETNIFFQKPKGEIKTVIKWP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-13" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Z1</accession>
    <name>SYY_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="1">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tyrS</name>
        <name type="ordered locus">TV1019</name>
        <name type="ORF">TVG1042450</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917247.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Z1"/>
    <dbReference type="SMR" id="Q979Z1"/>
    <dbReference type="STRING" id="273116.gene:9381812"/>
    <dbReference type="PaxDb" id="273116-14325257"/>
    <dbReference type="GeneID" id="1441129"/>
    <dbReference type="KEGG" id="tvo:TVG1042450"/>
    <dbReference type="eggNOG" id="arCOG01886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035267_1_1_2"/>
    <dbReference type="OrthoDB" id="8389at2157"/>
    <dbReference type="PhylomeDB" id="Q979Z1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02009">
        <property type="entry name" value="Tyr_tRNA_synth_type4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023678">
        <property type="entry name" value="Tyr-tRNA-ligase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000240274" description="Tyrosine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="218"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02009"/>
        </source>
    </evidence>
    <sequence length="331" mass="37690" checksum="869D3A62B97D622B" modified="2001-10-01" version="1">MLIDKLMKNTREIVTLEDAQKLDQKAGVKSYIGIEPSGIPHVATAVMWPRKLAELQDDIKIYVLLADWHAMINNKLHGDLDLIRKGGELLKRSFMAEGLTKAEYLWASQLVSSSNYWEMFIKTAKRSTLKRLTRALPIMGRTEADAEKDFSMYIYPIMQVTDIFYLDVDIAFGGMDQRHAHMLARDIADKMKVKKAVSVHGYLLSSLKGNTRMDNFVKMSKSDPNSAILINDEYKDIERKVNAAFCPPEKVDGNPLAEIMKYVLIPYYGRDIIIEKPSGNVRIENVDQFQNDYISGKIAPTELKKAMIPILDEMIEPARKVAYDMDLSIFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Z4</accession>
    <name>SAHH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="1">3.13.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">S-adenosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="1">AdoHcyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ahcY</name>
        <name type="ordered locus">TV1016</name>
        <name type="ORF">TVG1039754</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="1">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Z4"/>
    <dbReference type="SMR" id="Q979Z4"/>
    <dbReference type="STRING" id="273116.gene:9381809"/>
    <dbReference type="PaxDb" id="273116-14325254"/>
    <dbReference type="KEGG" id="tvo:TVG1039754"/>
    <dbReference type="eggNOG" id="arCOG04137">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025194_2_1_2"/>
    <dbReference type="OrthoDB" id="8479at2157"/>
    <dbReference type="PhylomeDB" id="Q979Z4"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071269">
        <property type="term" value="P:L-homocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000117018" description="Adenosylhomocysteinase">
        <location>
            <begin position="1"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="143"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="206"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="285"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <sequence length="410" mass="45805" checksum="8F59DDCD27286626" modified="2001-10-01" version="1">MAEGKGFLRLEWARDHMPVIAEIRKRFLDEKPFKGINIAMALHVEAKTGIFSLLLKEGGANVRMASCNPLSSDDSVVESLKTDYGMPVFARKGETQEEYYENLQRTLEKPPDIIIDDGGDLTKLVHTERKDLSKNIMGGNEETTTGVVRLKAMEKAGVLLFPMFDVNDANMKHLFDNRYGTGQSTLDGIMNSTNLLIAGRNVVVAGYGYCGRGIAMRLKGMGANVIVTEIDPIKANEAIMDGFQVRRMNDAIRYADMVITATGMKDVVKYEDALVAKKNIVLANAGHFDNEVAVKEIEKHSLEKREVREFVKRYRLENGNTVDVIADGRLVNLAAGQGHPVEIMDLSFALQALTAEYLVKNHQNLEKKVYPVPPEIDRYVAEIRLKQFGGELDTLTEDQIKYLNSWDEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A34</accession>
    <name>Q97A34_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRC-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0999116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054010.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A34"/>
    <dbReference type="PaxDb" id="273116-14325193"/>
    <dbReference type="GeneID" id="24779957"/>
    <dbReference type="KEGG" id="tvo:TVG0999116"/>
    <dbReference type="HOGENOM" id="CLU_2340349_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="97" mass="11001" checksum="402243D2001036EA" modified="2001-10-01" version="1">MMDFYSRLIEKPVMSSSGEIIGHVKNFSIDEKWNIVTMIASPASKKYENLPTDDDGNFLIPMANVNVGKDAIVVMNIESIKRSVKQDQGIDPQWFHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="145" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A35</accession>
    <name>Q97A35_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cell cycle control protein 48</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0998758</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917205.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A35"/>
    <dbReference type="STRING" id="273116.gene:9381767"/>
    <dbReference type="PaxDb" id="273116-14325192"/>
    <dbReference type="GeneID" id="1442053"/>
    <dbReference type="KEGG" id="tvo:TVG0998758"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_3_2"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="PhylomeDB" id="Q97A35"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19529">
        <property type="entry name" value="RecA-like_VCP_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000038">
        <property type="entry name" value="spermatogenesis-associated protein 5-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="7">
        <location>
            <begin position="8"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="6">
        <location>
            <begin position="111"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="223"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="500"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="745" mass="82999" checksum="0750D1C63A3E8986" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A44</accession>
    <name>Q97A44_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hypohtetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0990507</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A44"/>
    <dbReference type="STRING" id="273116.gene:9381758"/>
    <dbReference type="PaxDb" id="273116-14325183"/>
    <dbReference type="KEGG" id="tvo:TVG0990507"/>
    <dbReference type="eggNOG" id="arCOG02099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_0_2_2"/>
    <dbReference type="OrthoDB" id="24735at2157"/>
    <dbReference type="PhylomeDB" id="Q97A44"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="entry name" value="Iron dependent repressor, metal binding and dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="C-terminal domain of transcriptional repressors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferrous iron transporter FeoA-like" evidence="3">
        <location>
            <begin position="147"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="219" mass="25042" checksum="87515142FCA79D24" modified="2001-10-01" version="1">MKVMRVRSVTEEDYLKIIQELVLYQDYAQLSDIAKSLQVKRQSARDEINHLIDLGMVKKIDRGKYVLTDQGDIEANIFLRKHRTAEVLLSQCVGIEWDAVDREAMGIEHGMTEEIIHRVIERFNAKRCPHGNPIPSPEGYVEKIDDVRITDLNETGDVIISRIIYETDEILNYLYANQILPGAKVKIIRTGFPLQIEKDGRLLSLDPSIAKAIRVSSIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A49</accession>
    <name>ARCH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein archease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0961</name>
        <name type="ORF">TVG0986240</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archease family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917191.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A49"/>
    <dbReference type="SMR" id="Q97A49"/>
    <dbReference type="STRING" id="273116.gene:9381753"/>
    <dbReference type="PaxDb" id="273116-14325178"/>
    <dbReference type="GeneID" id="1442039"/>
    <dbReference type="KEGG" id="tvo:TVG0986240"/>
    <dbReference type="eggNOG" id="arCOG04055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111362_3_0_2"/>
    <dbReference type="OrthoDB" id="8831at2157"/>
    <dbReference type="PhylomeDB" id="Q97A49"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="entry name" value="Archease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01222">
        <property type="entry name" value="Archease_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022952">
        <property type="entry name" value="Archease_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001617">
        <property type="entry name" value="PRK00407.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682:SF11">
        <property type="entry name" value="PROTEIN ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="MTH1598-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0106">Calcium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000068856" description="Protein archease">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01222"/>
        </source>
    </evidence>
    <sequence length="133" mass="15514" checksum="B23C4A1B4413A7F9" modified="2001-10-01" version="1">MTYEILDHESDIGIMVYGTTYEELFSNAVYAMADLILDVGKLKEKRKMHEIIRGNTPEDIMVNLLSRVLFYVDTYYTLYFRATCKYEDSTLDVYLYGSEIPEEVEYRNVIKAVTYSEIAVKPEDGFARVIFDL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-31" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A76</accession>
    <name>PIP_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Proline iminopeptidase</fullName>
            <shortName>PIP</shortName>
            <ecNumber>3.4.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Prolyl aminopeptidase</fullName>
            <shortName>PAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Tricorn protease-interacting factor F1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pip</name>
        <name type="ordered locus">TV0934</name>
        <name type="ORF">TVG0960913</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves H-Pro-AMC as well as a wide spectrum of amino acid substrates and several peptide substrates without a proline at the N-terminus. In conjunction with the three factors F1, F2 and F3, Tricorn degrades oligopeptides in a sequential manner, yielding free amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Release of N-terminal proline from a peptide.</text>
            <dbReference type="EC" id="3.4.11.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the tricorn proteolytic complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.5"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A76"/>
    <dbReference type="SMR" id="Q97A76"/>
    <dbReference type="STRING" id="273116.gene:9381726"/>
    <dbReference type="ESTHER" id="thevo-pip">
        <property type="family name" value="Proline_iminopeptidase"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325151"/>
    <dbReference type="KEGG" id="tvo:TVG0960913"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_15_1_2"/>
    <dbReference type="PhylomeDB" id="Q97A76"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002410">
        <property type="entry name" value="Peptidase_S33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005945">
        <property type="entry name" value="Pro_imino_pep"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01250">
        <property type="entry name" value="pro_imino_pep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045948">
        <property type="entry name" value="ProImpepThrmp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF31">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN YCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005539">
        <property type="entry name" value="Pept_S33_TRI_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00793">
        <property type="entry name" value="PROAMNOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000080852" description="Proline iminopeptidase">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="35"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="295" mass="33830" checksum="821B4C15E3EDD860" modified="2001-10-01" version="1">MRKLSRCEDGYVKIQGIYIYYKVCKAENEKAKLMTLHGGPGMSHDYLLSLTDLAEKGITVLFYDQFGCGRSEEPEKEKFTIDYGVEEAEAVKKNIFGDDKVFLMGSSYGGALALAYAVKYQAHLKGLIISGGLSSVPLTVKEMQRLIDELPEKYRNAIRKYGEVGDYQNPEYQEAVNYFYHQHLLRSEDWPPEVLKSLEYAEERNVYRTMNGPNEFTITGTIRDWDITDKIGIISVPTLITVGEFDEVTQNVAEVIHSKIDNSQLIVFKACSHLTMWEDRDEYNRILLQFIEKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A78</accession>
    <name>Q97A78_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="3">2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Alpha-IPM synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0959514</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917167.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A78"/>
    <dbReference type="STRING" id="273116.gene:9381724"/>
    <dbReference type="PaxDb" id="273116-14325149"/>
    <dbReference type="GeneID" id="1442015"/>
    <dbReference type="KEGG" id="tvo:TVG0959514"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_4_2_2"/>
    <dbReference type="OrthoDB" id="6555at2157"/>
    <dbReference type="PhylomeDB" id="Q97A78"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03174">
        <property type="entry name" value="DRE_TIM_metallolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF9">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="344" mass="38025" checksum="96803D5E3EE65893" modified="2001-10-01" version="1">MLTDDTLREGMQAPGIAFTLDEKIRIAQSISKSGIKRALVSYPSAHESEVEAARKITDLHLFDDTYSLGRSIKEDVDSIISTGSKVTLHLPFGDYDINKICDVVKYASDHSDSVEIAIVDMVKYEKEKIFDLAKKLWECGASIVQIPDTTGRATPKRVHDVVSYIKQNLKVKVEVHCHNDHGLAIANSIAGIEAGCDYVDTTIFGIGERNGIADTASIASYLEKAGYEDKINFEMLRKTYDLMSELILRKAGYRFFADNMPIFGRNVLSITAGTHSTSKVFPNSRVSLNVYAGSRLVASILRKNGIEPDERKVKLLVSKIKDLSSNEGRVVGEDEVLKIYGGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A84</accession>
    <name>Q97A84_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0954446 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0954446</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A84"/>
    <dbReference type="PaxDb" id="273116-14325143"/>
    <dbReference type="KEGG" id="tvo:TVG0954446"/>
    <dbReference type="HOGENOM" id="CLU_3021186_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="55" mass="6162" checksum="ECA63C6AAF52ECDA" modified="2001-10-01" version="1">MLYLIGGEDTSSEQEGLSKNAMGLPGLILVSLTLERYQSMVYQLVLIKTIKYPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A89</accession>
    <name>Q97A89_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dTDP-4-dehydrorhamnose 3,5-epimerase</fullName>
            <ecNumber evidence="3">5.1.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Thymidine diphospho-4-keto-rhamnose 3,5-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0949657</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose</text>
            <dbReference type="Rhea" id="RHEA:16969"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="5.1.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.13" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917159.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A89"/>
    <dbReference type="STRING" id="273116.gene:9381713"/>
    <dbReference type="PaxDb" id="273116-14325138"/>
    <dbReference type="GeneID" id="1442007"/>
    <dbReference type="KEGG" id="tvo:TVG0949657"/>
    <dbReference type="eggNOG" id="arCOG04188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090940_1_1_2"/>
    <dbReference type="OrthoDB" id="2990at2157"/>
    <dbReference type="PhylomeDB" id="Q97A89"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00438">
        <property type="entry name" value="cupin_RmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="RmlC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01221">
        <property type="entry name" value="rmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047">
        <property type="entry name" value="DTDP-6-DEOXY-D-GLUCOSE-3,5 EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047:SF2">
        <property type="entry name" value="THYMIDINE DIPHOSPHO-4-KETO-RHAMNOSE 3,5-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="site" description="Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="179" mass="21056" checksum="962F3D0D5B9494AD" modified="2001-10-01" version="1">MTFIFEETEIPDVIHIVCNRYEDNRGYFEERYKASEFDRILHVRFVQDNHSFSRRGVIRGLHFQREPMAQGKLVGVITGRIYDVAVDLRKDSVTFGKWVYRVLSNNEMLWIPRGFAHGFQALEDSHVVYKVDNEFSRQHEDGIIFNDPDLAIDWPIKDPIVSEKDIKLGTFRNFRGSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A92</accession>
    <name>Q97A92_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0946674</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A92"/>
    <dbReference type="STRING" id="273116.gene:9381710"/>
    <dbReference type="PaxDb" id="273116-14325135"/>
    <dbReference type="KEGG" id="tvo:TVG0946674"/>
    <dbReference type="eggNOG" id="arCOG03651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_20_3_2"/>
    <dbReference type="PhylomeDB" id="Q97A92"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="367"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="38"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="475" mass="51294" checksum="0AED1D3A31038D96" modified="2001-10-01" version="1">MIMSSNTYTLKEGVIGEFRLVWQAMGALSIAADASYLLTGVALFALGATPLSILLGVVFYLFIMNSGYQFSKYISSAGSYYTFAAKSLGPKIGIFQAWNMIFYSILGYSSFGFLGLAAFISMINPSLNSTAVWVTIAVAAATIAFLFTYFGIKTSTDYQILGGVIEVSVLIIGSILIIISAGNSNTLSVFTTKYIPGGLSQIFYSMIYSVVLFFGTTLSITSLAEEAKNPHIAVRRALISTIVVAGITLILVSYAFTIGYGPTNMGAFIKSPDPGLVMFKHVSYILYILLIAVTVNSFMGYNVSVSNANTRIYYSFARSGILFLPRSLKKIHERYGSPYNSAIFVFIVSLSVGLAFGFAFGPEIGGLVMLFANGYAAYTEHIVASIGLPFYAKKMGDFKVLQHLVFPIIAVGVLAAVIFFTLYPAPPAYPYNIAAYVGIGWIPFAGLMTLIELKKRGISNTLETEPEVAKTAENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A96</accession>
    <name>Q97A96_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0940537</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917152.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A96"/>
    <dbReference type="STRING" id="273116.gene:9381706"/>
    <dbReference type="PaxDb" id="273116-14325131"/>
    <dbReference type="GeneID" id="1442000"/>
    <dbReference type="KEGG" id="tvo:TVG0940537"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_661599_0_0_2"/>
    <dbReference type="OrthoDB" id="29061at2157"/>
    <dbReference type="PhylomeDB" id="Q97A96"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050189">
        <property type="entry name" value="MFS_Efflux_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124:SF3">
        <property type="entry name" value="CHLORAMPHENICOL EFFLUX PUMP RV0191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124">
        <property type="entry name" value="PURINE EFFLUX PUMP PBUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="18"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="409" mass="44230" checksum="A25A7A946FE53D6B" modified="2001-10-01" version="1">MQSTRNIVSNNKIWMLIIFVVGAIPALVSDFDGSAYTYAAPYILSRISLPTFYFGILISGYAAGIAIFSLVGGFMFDRFSPKSTVVYSVLLFSIFTILTGYSTNAIELVISRVLVGFGVGMFQSASVSFLGDANPETRGAGVMVWGVLAGLGTFIAPYIILPFLPNYRIPFLISGILGFIVAAVFYFVVPPVFKKEEKLKNPFRHVLNRYSVFPMVSMFFFGFTLLGLLGYYSDFLSSVVGFGKTSVAIIISFLGVGGVIFGIPAGIFSDMVGRKIVLIIAITLISISMLAITYAPRDLILFLLATLAFGTGWSIFSILSPATGQDMVRDEAVGSVSGAILMTFNIGGIIGPLMIGLILAKGFTLRAGFLYFIDIPAILALLLTIAMKYPKNIKEIVKQQLLEDASISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AC4</accession>
    <name>Q97AC4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0903758 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0903758</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053997.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AC4"/>
    <dbReference type="STRING" id="273116.gene:9381678"/>
    <dbReference type="PaxDb" id="273116-14325103"/>
    <dbReference type="GeneID" id="24779945"/>
    <dbReference type="KEGG" id="tvo:TVG0903758"/>
    <dbReference type="HOGENOM" id="CLU_1187838_0_0_2"/>
    <dbReference type="OrthoDB" id="378829at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="233" mass="28163" checksum="7C0B9C9F98621BC7" modified="2001-10-01" version="1">MKDNKMKLNIYDVVFVLPRPSITYPSGGYDIVYRLAKALNKEGIRTSIIFLEKPEIYIENYNVSQHVKNENRWYRLIVYLFRLLFNVKRIDLFYKLRLYKLLGVDYNYSILDDIDLYYYETIEDVKIKINIIIATSWETAYFVREYVKKHKIETLYLVQHSQDDVSFSGENSNNAKFTYTFPFKKIVINQKMYNRFKAEKPLFFHVGIDTQFFKKLTKTNDREYIMFPLRISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AE2</accession>
    <name>Q97AE2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dolichol-phosphate mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0887952</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760251.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AE2"/>
    <dbReference type="STRING" id="273116.gene:9381660"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325085"/>
    <dbReference type="GeneID" id="1441960"/>
    <dbReference type="KEGG" id="tvo:TVG0887952"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_13_1_2"/>
    <dbReference type="PhylomeDB" id="Q97AE2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="4">
        <location>
            <begin position="10"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="251" mass="28236" checksum="ED70864C99198B3E" modified="2001-10-01" version="1">MVICLSTEISLVAATLNEIGNIATFLDKVEKELIYYNFEIIIVDDNSSDGTKEYLAERSLKDKNLHVIENPYRVGMLGSLKMGINSAKGKYCIVMDADLQHPPEAILSIIERLNHGCDIVVGSRYVNGGSAGDRNSYRALLSIGAQYLSYLFLRSARSTTDPMSGFFGFQTSMNHDFSYKNRFKLTDMGAKILLLLLAENQNCNVCDVGYVFRSRERGKSKIVSGMSFLERYMSELLDAMIVERQWKNRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AE3</accession>
    <name>Q97AE3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0886521 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0886521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917111.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AE3"/>
    <dbReference type="STRING" id="273116.gene:9381659"/>
    <dbReference type="PaxDb" id="273116-14325084"/>
    <dbReference type="GeneID" id="1441959"/>
    <dbReference type="KEGG" id="tvo:TVG0886521"/>
    <dbReference type="eggNOG" id="arCOG01943">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1418735_0_0_2"/>
    <dbReference type="OrthoDB" id="56796at2157"/>
    <dbReference type="PhylomeDB" id="Q97AE3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="1">
        <location>
            <begin position="34"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="194" mass="22168" checksum="6C68AD85948F31E0" modified="2001-10-01" version="1">MERYDGADLDPGHSILVIMNATEENFKHIFSKLEFITSMKVIEDAMKLYNIPAVFIERNFRSSRLSDYIARKYSQKMVSLMSPSSYDEEYIRQFGELAIPPGLSIGSEDIFSDNTVRNMIEGSGKTTVVLAGFYTESDIYISAIESLLHDYYTFVISDATSTISERTYFEALDLLSQHVEVIDSRDLIRYWSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AH9</accession>
    <name>Q97AH9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG0848756</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the Lhr helicase family. Lhr-Core subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917075.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AH9"/>
    <dbReference type="STRING" id="273116.gene:9381623"/>
    <dbReference type="PaxDb" id="273116-14325048"/>
    <dbReference type="GeneID" id="1441923"/>
    <dbReference type="KEGG" id="tvo:TVG0848756"/>
    <dbReference type="eggNOG" id="arCOG00557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_0_0_2"/>
    <dbReference type="OrthoDB" id="33870at2157"/>
    <dbReference type="PhylomeDB" id="Q97AH9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="Lhr-like_DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF5">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="WHD_Lhr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="32"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="247"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB59973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="894" mass="101562" checksum="E87D5397FCAF9BED" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-31" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AJ6</accession>
    <name>TRF3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Tricorn protease-interacting factor F3</fullName>
            <ecNumber>3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trf3</name>
        <name type="ordered locus">TV0814</name>
        <name type="ORF">TVG0817891</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Proteases F1, F2 and F3 degrade oligopeptides produced by Tricorn (themselves probably produced by the proteasome), yielding free amino acids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the tricorn proteolytic complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917058.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AJ6"/>
    <dbReference type="SMR" id="Q97AJ6"/>
    <dbReference type="STRING" id="273116.gene:9381604"/>
    <dbReference type="MEROPS" id="M01.021"/>
    <dbReference type="PaxDb" id="273116-14325030"/>
    <dbReference type="GeneID" id="1441906"/>
    <dbReference type="KEGG" id="tvo:TVG0817891"/>
    <dbReference type="eggNOG" id="arCOG02969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003705_0_1_2"/>
    <dbReference type="OrthoDB" id="139771at2157"/>
    <dbReference type="PhylomeDB" id="Q97AJ6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00142">
        <property type="entry name" value="ZINC_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000095113" description="Tricorn protease-interacting factor F3">
        <location>
            <begin position="1"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="231"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="779" mass="88069" checksum="6BC227F2ECECB201" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AJ8</accession>
    <name>Q97AJ8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Maturation protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0814159</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AJ8"/>
    <dbReference type="STRING" id="273116.gene:9381602"/>
    <dbReference type="PaxDb" id="273116-14325028"/>
    <dbReference type="DNASU" id="1441904"/>
    <dbReference type="KEGG" id="tvo:TVG0814159"/>
    <dbReference type="eggNOG" id="arCOG00321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_2_2"/>
    <dbReference type="PhylomeDB" id="Q97AJ8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF11">
        <property type="entry name" value="ZINC-DEPENDENT PROTEASE, TLDD_PMBA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="5">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="7">
        <location>
            <begin position="93"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="6">
        <location>
            <begin position="204"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="444" mass="50034" checksum="1DEC210608D15B87" modified="2001-10-01" version="1">MQIRRTRYMDITSRGAFYRNGEFDGSSSSRGSGYAIRVVNDSIAIAYSNSDDWERVKKRIDEALDRSRKIGKSKIFTGRDVRSSWTKLGNKKVLDTDFNEIVDILKEADTAASNVAQLRINSISDKIVKEYYMNTEGSEVHGEYSRIYFQYLIGVVESGNYEQSSEEYGYTGGYETFDLGRIAEEEREDAVKLREIALAPSVEPGKFDVIIGPEISGIVAHESAGHPTEYDRIAGREAVLAGESFLSGKKFPYKIGSELVNVVDDPTVENSFGYYLYDDEGVPARKRYLYKDGMTSEFLLNRESAAWAGTESNGAGRSSSWDMEPLARMSTTYIEPGDYSFEELFEGVRRGVYIKSFTEWNIDDLRFNEKYVGKEAYYIENGDIKGRVRRPVLETNTVNFYSSVDAVGKDLHFNAGICGKGDPEQGVEVWMGGPHLRLRGMIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AL1</accession>
    <name>Q97AL1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TIGR00725 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0800469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917043.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AL1"/>
    <dbReference type="STRING" id="273116.gene:9381589"/>
    <dbReference type="PaxDb" id="273116-14325015"/>
    <dbReference type="GeneID" id="1441891"/>
    <dbReference type="KEGG" id="tvo:TVG0800469"/>
    <dbReference type="eggNOG" id="arCOG02431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107614_1_1_2"/>
    <dbReference type="OrthoDB" id="9570at2157"/>
    <dbReference type="PhylomeDB" id="Q97AL1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005268">
        <property type="entry name" value="CHP00725"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041164">
        <property type="entry name" value="LDcluster4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052341">
        <property type="entry name" value="LOG_family_nucleotidases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00725">
        <property type="entry name" value="TIGR00725 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43393">
        <property type="entry name" value="CYTOKININ RIBOSIDE 5'-MONOPHOSPHATE PHOSPHORIBOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43393:SF3">
        <property type="entry name" value="LYSINE DECARBOXYLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18306">
        <property type="entry name" value="LDcluster4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="17968" checksum="1FDD5410BFA0E06C" modified="2001-10-01" version="1">MPELNIAVIGGSRVSQEVCTLSYNVGRILAKYNVVVFCGGLTGVMECVSKGVSEGNGIVVGILPGYDPSEANDYVTIKVPTGMGYMRNFLIIRASQAVISIDGSAGTLSEASFAISEGKDVVSLFELNIPRKKEKEGHVYVEPDPEKAVQMAIELAKNWKWHEENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AP6</accession>
    <name>Q97AP6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaeal Type IV pilin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0767609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917011.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AP6"/>
    <dbReference type="STRING" id="273116.gene:9381554"/>
    <dbReference type="PaxDb" id="273116-14324980"/>
    <dbReference type="DNASU" id="1441859"/>
    <dbReference type="GeneID" id="25392633"/>
    <dbReference type="KEGG" id="tvo:TVG0767609"/>
    <dbReference type="eggNOG" id="arCOG07380">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718302_0_0_2"/>
    <dbReference type="OrthoDB" id="57426at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012859">
        <property type="entry name" value="Pilin_N_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07790">
        <property type="entry name" value="Pilin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal Type IV pilin N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="150" mass="16391" checksum="B68AE25F5B9F6DC3" modified="2001-10-01" version="1">MEDKAVSPIIATILLIAITVALAATFYTEVTPYFTEAQYYTPQAVTDVMNVSNGSSFSYDIYIQFFPANLTASQVEISLHVGSSYEYINLKMLSPFGKTTLNNGSITISQYPSAGYFTQGLIFVINSTLPLHSIIFVDLKTSSSINTVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AQ8</accession>
    <name>Q97AQ8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0758219</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">The Mn(2+) ion enhances activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AQ8"/>
    <dbReference type="STRING" id="273116.gene:9381541"/>
    <dbReference type="MEROPS" id="M20.008"/>
    <dbReference type="PaxDb" id="273116-14324967"/>
    <dbReference type="KEGG" id="tvo:TVG0758219"/>
    <dbReference type="eggNOG" id="arCOG01108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_0_1_2"/>
    <dbReference type="PhylomeDB" id="Q97AQ8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000014">
        <property type="entry name" value="N-acyl-L-amino acid amidohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053493">
        <property type="entry name" value="Thermostable_CP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040868">
        <property type="entry name" value="carboxypep_CpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF63">
        <property type="entry name" value="METALLOPEPTIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G09600)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="6">
        <location>
            <begin position="199"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005962-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="404" mass="44080" checksum="4D6BBE73321C7327" modified="2001-10-01" version="1">MKPFANRCMDHAAFEKEIDEFNERMIEIRRDLHEHPELSYKEVRTAKLVADTLRSFGIEVRENVGGTGVVGLLRGKKGNVTIGLRADMDALPVQEQTGLPFASKNSGVMHACGHDSHVAMLIGAAYVLSKHGDELDGNVKFLFQPAEEDGGRGGALPMIEDGALENPHVDHVFGLHVLGDFPAGYFAIRGGPIMAAPDSFKIEVHGRGGHGSAPWDTVDPIFVSSQIIQALYGMRSRNVDQRDPLVISVCSVHSGTKDNIIPDNALLEGTLRTLDEDVRADMKKKISNTAEAVAGAFGAKADVSFIENAYPVTYNDPAITEEVKSILSSIKGMKTMETKPLLGGEDVSRFLQRAPGTYYFLGTRNEKKGIIYPNHSSKFTVDEDYLKYGALSHVLVAMHFAGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AS8</accession>
    <name>Q97AS8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0737684 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0737684</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916982.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AS8"/>
    <dbReference type="SMR" id="Q97AS8"/>
    <dbReference type="STRING" id="273116.gene:9381520"/>
    <dbReference type="PaxDb" id="273116-14324947"/>
    <dbReference type="GeneID" id="25392632"/>
    <dbReference type="KEGG" id="tvo:TVG0737684"/>
    <dbReference type="eggNOG" id="arCOG00724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143277_0_0_2"/>
    <dbReference type="OrthoDB" id="34535at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="165" mass="19437" checksum="330439F902C2B2D0" modified="2001-10-01" version="1">MINVKIETDWDKLLSDYENSKELNVEDIILLLLNYGKISGKTMMQKQVFLTVKEVFTNISALYHPDKYGPYSQLVSDIVVKLINDGKIIKFNRGEGHATYAITESGKEYINKVIKSKNINNEDLEKLRKNKYDWDEWDTKGILRYVYRNYPEYATKTKVPELKWE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-16" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AU0</accession>
    <name>Y719_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein TV0719</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0719</name>
        <name type="ORF">TVG0726109</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MacroD-type family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916977.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AU0"/>
    <dbReference type="SMR" id="Q97AU0"/>
    <dbReference type="STRING" id="273116.gene:9381508"/>
    <dbReference type="PaxDb" id="273116-14324935"/>
    <dbReference type="GeneID" id="1441825"/>
    <dbReference type="KEGG" id="tvo:TVG0726109"/>
    <dbReference type="eggNOG" id="arCOG04225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046550_5_1_2"/>
    <dbReference type="OrthoDB" id="57099at2157"/>
    <dbReference type="PhylomeDB" id="Q97AU0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02908">
        <property type="entry name" value="Macro_OAADPr_deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001664">
        <property type="entry name" value="PRK00431.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF27">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000089240" description="Uncharacterized protein TV0719">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="186" mass="20229" checksum="A72B9E04CD6E1B0A" modified="2001-10-01" version="1">MVSFSYKGNLIEIIEGDITDVNCEAIVNAANPSLMGGGGVDGAIHLKGGKTIDLECAELRRTKWPKGLPPGEADITSGGKLKAKYVIHTVGPIYRGQEEDAETLYSSYYRSLEIAKIHGIKCIAFPAISTGIYGYPFEEASVIALKAVTDFLSNKEGYIIKFVLYGQARYQTFVSLASDFLMAYNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AX1</accession>
    <name>Q97AX1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0696684 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0696684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AX1"/>
    <dbReference type="STRING" id="273116.gene:9381476"/>
    <dbReference type="PaxDb" id="273116-14324904"/>
    <dbReference type="KEGG" id="tvo:TVG0696684"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451487_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="114"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="186" mass="21566" checksum="B3F1AE90317D7C03" modified="2001-10-01" version="1">MKKHFNKIIKNKILKKHCNKISEESKIEGIGMNTYSKKFNLSNHLLILVTGIINKKDLTDTAYRNNISKSQLSKLNNNRSHNIFENMFYSILNAFIKAHKYDVYKDIIDKLFNIITIDSTFIETLIKGSGIDQREKRKNGIKLHISALTNIKLPLKSIITLANIHDTKIFDALLNNIKEYILTIQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AX9</accession>
    <name>Q97AX9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0680586 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0680586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053952.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AX9"/>
    <dbReference type="PaxDb" id="273116-14324896"/>
    <dbReference type="GeneID" id="24779908"/>
    <dbReference type="KEGG" id="tvo:TVG0680586"/>
    <dbReference type="eggNOG" id="arCOG01242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340348_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="97" mass="11200" checksum="613625C9F5DD89CF" modified="2001-10-01" version="1">MDQETTNKEIGRLENSDIPRVVKDKIREFVDDILLSGLSLNRQYFYAIRLRQIASLIPDKFLNPSTADIKRVMSTLMSGKIGRRIKGKNHSSVYSDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AY5</accession>
    <name>Q97AY5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Formylmethanofuran dehydrogenase subunit E domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0674383</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_156769062.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AY5"/>
    <dbReference type="STRING" id="273116.gene:9381462"/>
    <dbReference type="PaxDb" id="273116-14324890"/>
    <dbReference type="GeneID" id="1441782"/>
    <dbReference type="KEGG" id="tvo:TVG0674383"/>
    <dbReference type="eggNOG" id="arCOG00762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087508_0_0_2"/>
    <dbReference type="OrthoDB" id="31120at2157"/>
    <dbReference type="PhylomeDB" id="Q97AY5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3320.10">
        <property type="entry name" value="pa2218 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003814">
        <property type="entry name" value="FmdEsu_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023288">
        <property type="entry name" value="Pa2218-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053194">
        <property type="entry name" value="tRNA_methyltr_O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39418:SF1">
        <property type="entry name" value="DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39418">
        <property type="entry name" value="DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02663">
        <property type="entry name" value="FmdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143555">
        <property type="entry name" value="FwdE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formylmethanofuran dehydrogenase subunit E" evidence="4">
        <location>
            <begin position="37"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="233" mass="26883" checksum="56AE7AD4BCDBBD9E" modified="2001-10-01" version="1">MESERNKIEHPGNSESQTQQKILESSKVLIPEWAYTFHGHKCPYMPLGYRAGLYAMKLLKIDREKDHKTLIYSEMSEKDLNGCFNDGLQAATGCTYGKGLLNLLGYGKLALILYRKDKGAVRVHVRDEIMDELSSRATEFFSLRKSGKEPSEIPDEFIDPIINDWMPSLSDEQMFEYKFIKDFHPIPAKKSGVKKKCDQCHEYVYEIDLVPSEGKLICKPDYYNVDENHPVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AY6</accession>
    <name>Q97AY6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA mismatch repair protein [MutS]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0672842</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916933.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AY6"/>
    <dbReference type="STRING" id="273116.gene:9381461"/>
    <dbReference type="PaxDb" id="273116-14324889"/>
    <dbReference type="GeneID" id="1441781"/>
    <dbReference type="KEGG" id="tvo:TVG0672842"/>
    <dbReference type="eggNOG" id="arCOG02897">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036487_1_0_2"/>
    <dbReference type="OrthoDB" id="15514at2157"/>
    <dbReference type="PhylomeDB" id="Q97AY6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF34">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="4">
        <location>
            <begin position="323"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="500" mass="56349" checksum="097EA10A34F88060" modified="2001-10-01" version="1">MNVDLLFNDTDGRVNESVPWNSNDLEKDLDLEILYNVMAGKDTYVFDVCRRTILNSVNDKSTIIYRQDVLKDAIRNQNIIRKIYSTIVTAESEAKRSYFWISRSNPEFALHESISILKIYLTAIENIRDVGREALPLLHSNGLKQLFTMIMQEFNHEYVAEVLNHLEILRFHNGISVRGSLGFANSLIGYNLLMPDAKSNKILDKISLIRERHYTYVLPDRDEIGAQELADMRAKSIRETAAILTESAQNVLAFIDRIKEGVAFYLGCLNLLDELTKIGAPISFPVPENNENNGMYYTGIYDVALCLRTKTVPVRNSLSAEHDSIIFITGANRGGKSTLLRAIGQAQLMMQCGMFVAAESFSAYIASGIFTHFKREEDSGMSMGKLEEEMNRMSLIIDHIKAGGRLFLNESFSSTNVREGSEIAKQVINALLDSGVKVIFVTHFNELAESYLGSVYNPTFLRAERLDNGKRTFKILQADPITTSFGYDIFLKVFGESAKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AY7</accession>
    <name>Q97AY7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mismatch binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0671343</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AY7"/>
    <dbReference type="STRING" id="273116.gene:9381460"/>
    <dbReference type="PaxDb" id="273116-14324888"/>
    <dbReference type="KEGG" id="tvo:TVG0671343"/>
    <dbReference type="eggNOG" id="arCOG02896">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036487_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AY7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF34">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="4">
        <location>
            <begin position="329"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="509" mass="58527" checksum="1F785CB66BCB12FD" modified="2001-10-01" version="1">MIEMVFNSILWPDKKAESLFDSDPDFFADLNLDMIIDGITSGYTDYNLRPFFITMPRSLDTVKYRQEIFKDLLNSEVFALMKDFSEKMNTIYKWVSTADRLGGYQKQGWLLEAALFYFNTMADFSLKLDNLDFKSQGLLMLREYLRELVHTDAFIVASREANTVKSLLSAIRYEMLIESSKVTIRRDKGKDDYNEIVKKLFDKFTNRNATSKSLKRFYETSMNQVEAAVLELIARLFPEEFKALSSFYENHKNFIDPTTAQMYRELQFYLSYIEYISPLEKTGLKFCIPEVGTVRDIYCIECFDLALAQRLLQSGSVVIANDFRLSRNENIIVVTGPNSGGKTTFARSIGQVFFLAMLGLPIPGSKAKLFQVDNIYTHFEKSEDLENLRGKLEDDLIRMRKILDSATERSLIIINEMLSSTASKDAISIGTKIIDLINKKNSICVYVTFFEELARIRGVISMVSQVDPIAPEIRTFRVVRSDPNGLAYATAIAERYGLTYKKIIERVRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AZ4</accession>
    <name>TAL_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Probable transaldolase</fullName>
            <ecNumber>2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">tal</name>
        <name type="ordered locus">TV0665</name>
        <name type="ORF">TVG0660430</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transaldolase family. Type 3B subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916924.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AZ4"/>
    <dbReference type="SMR" id="Q97AZ4"/>
    <dbReference type="STRING" id="273116.gene:9381453"/>
    <dbReference type="PaxDb" id="273116-14324881"/>
    <dbReference type="GeneID" id="1441772"/>
    <dbReference type="KEGG" id="tvo:TVG0660430"/>
    <dbReference type="eggNOG" id="arCOG05061">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079764_0_0_2"/>
    <dbReference type="OrthoDB" id="6661at2157"/>
    <dbReference type="PhylomeDB" id="Q97AZ4"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00956">
        <property type="entry name" value="Transaldolase_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000018">
        <property type="entry name" value="Probable transaldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00494">
        <property type="entry name" value="Transaldolase_3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022999">
        <property type="entry name" value="Transaldolase_3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004731">
        <property type="entry name" value="Transaldolase_3B/F6P_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033919">
        <property type="entry name" value="TSA/FSA_arc/bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00875">
        <property type="entry name" value="fsa_talC_mipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF40">
        <property type="entry name" value="FRUCTOSE-6-PHOSPHATE ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0570">Pentose shunt</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000173692" description="Probable transaldolase">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="223" mass="24321" checksum="54B34DD383AD760C" modified="2001-10-01" version="1">MKIFLDTANISEIKEGIDLGLVDGVTTNPTLISKEAGGSKKYAEIIKEILKIVDGPVSVEVVSTKSDGMVEEARKIHALGDNAVVKIPMTDEGLKAIRKLSQENIETNCTLVFNPIQALLAAKSGATYVSPFVGRLDDIGQDGMQIIDEIKTIFNNYIIKTQILVASVRNPIHVLRAAIIGADVVTIPFSVLKLLIKHPKTDEGLTRFLEDWKKVSPDGKFII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-10" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B05</accession>
    <name>IF2B_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>aIF2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>eIF-2-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">eif2b</name>
        <name type="ordered locus">TV0654</name>
        <name type="ORF">TVG0649580</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59796" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59796.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916913.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B05"/>
    <dbReference type="SMR" id="Q97B05"/>
    <dbReference type="STRING" id="273116.gene:9381442"/>
    <dbReference type="PaxDb" id="273116-14324870"/>
    <dbReference type="GeneID" id="1441761"/>
    <dbReference type="KEGG" id="tvo:TVG0649580"/>
    <dbReference type="eggNOG" id="arCOG01640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026663_3_0_2"/>
    <dbReference type="OrthoDB" id="38099at2157"/>
    <dbReference type="PhylomeDB" id="Q97B05"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00232">
        <property type="entry name" value="eIF_2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004458">
        <property type="entry name" value="TIF2_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003067">
        <property type="entry name" value="PRK03988.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008993">
        <property type="entry name" value="PRK12336.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137437" description="Translation initiation factor 2 subunit beta">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM">
        <location>
            <begin position="144"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="208" mass="23524" checksum="519A6594425CB7E5" modified="2002-05-10" version="2">MTDSYDKLLEKAKDVLSSSTKNIDRLKIPEPEIIREGKATIIRNFQDIIDIINRDPDQVIKFLTRELGTNIVQNGRRLIINKKVTLEELQEKMNEYINTYVRCYECGSLDTEIQKSGRVSLLVCKACGAQHPIHTVKEFKEEEGIEEGKEYTVEISEVGSSGEGRASFRGFTIFVPGTKKGETVKVKIKKIKNDVAIAEVVSRTSDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B07</accession>
    <name>Q97B07_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0647983</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.88" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916911.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B07"/>
    <dbReference type="STRING" id="273116.gene:9381440"/>
    <dbReference type="PaxDb" id="273116-14324868"/>
    <dbReference type="GeneID" id="1441759"/>
    <dbReference type="KEGG" id="tvo:TVG0647983"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_2"/>
    <dbReference type="OrthoDB" id="6342at2157"/>
    <dbReference type="PhylomeDB" id="Q97B07"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000005">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050485">
        <property type="entry name" value="Proline_metab_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862:SF1">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="51"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="515" mass="57541" checksum="71AD447554B28084" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B19</accession>
    <name>ACTH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Archaeal actin homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0640</name>
        <name type="ORF">TVG0633900</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Polymerizes into bundles of filaments. Polymerization requires NTP and is optimal with ATP, but GTP, UTP, CTP, and even the deoxy form of NTP can also support the polymerization reaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thermophilic archaeal actin family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916899.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B19"/>
    <dbReference type="SMR" id="Q97B19"/>
    <dbReference type="STRING" id="273116.gene:9381428"/>
    <dbReference type="PaxDb" id="273116-14324856"/>
    <dbReference type="GeneID" id="1441747"/>
    <dbReference type="KEGG" id="tvo:TVG0633900"/>
    <dbReference type="eggNOG" id="arCOG03062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_851534_0_0_2"/>
    <dbReference type="OrthoDB" id="55553at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040607">
        <property type="entry name" value="ALP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049067">
        <property type="entry name" value="MreB-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17989">
        <property type="entry name" value="ALP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21522">
        <property type="entry name" value="MreB-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000285696" description="Archaeal actin homolog">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="285"/>
            <end position="288"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="326" mass="34970" checksum="FCAA0C004823B223" modified="2001-10-01" version="1">MVVIGLDVGYGDTKVIGIDGKRIIFPSRWAVTETESWGIGGKIPVLSTDGGQTKFIYGRYASGNNIRVPQGDGRLASKEAFPLIAAALWESGIHNDGSPVDLVIGSGTPLGTFDLEVKAAKEALENKILTVTGPEGEVRQYNVTRLIMRPQGVGAALYLLNQGIIEQQPGYGVVIDVGSRTTDVLTINLMDMEPVVELSFSLQIGVGDAISNLSRKIAKETGFVVPFDLAQEALSKPVMFRQKQVGGPEVAGPILEDLANRIIENIRLNLRGEVDRITSLIPVGGGANLIGDRFDEIAPGTTVRIKPEDLQFANALGYRDAAERSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B33</accession>
    <name>Q97B33_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH marR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0618226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916885.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B33"/>
    <dbReference type="STRING" id="273116.gene:9381414"/>
    <dbReference type="PaxDb" id="273116-14324842"/>
    <dbReference type="DNASU" id="1441733"/>
    <dbReference type="GeneID" id="1441733"/>
    <dbReference type="KEGG" id="tvo:TVG0618226"/>
    <dbReference type="eggNOG" id="arCOG04377">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163103_0_0_2"/>
    <dbReference type="OrthoDB" id="55633at2157"/>
    <dbReference type="PhylomeDB" id="Q97B33"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017185">
        <property type="entry name" value="UCP037373_trxn_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037373">
        <property type="entry name" value="UCP037373_trxn_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="120" mass="14191" checksum="7C7CA4DC77F835EE" modified="2001-10-01" version="1">MNMVLSEDAEKLAKYLMIADIPRSVAYTLVYIRDKDEVTSVEIERETGLRQPEVSIAMQWLRRKGWITKRNMKKEGKGRPIHGYRLSKSFSEILDEIIQDLSKKINDINYNIEQLKNYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B51</accession>
    <name>Q97B51_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flagella-related protein C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0599134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B51"/>
    <dbReference type="STRING" id="273116.gene:9381396"/>
    <dbReference type="PaxDb" id="273116-14324823"/>
    <dbReference type="KEGG" id="tvo:TVG0599134"/>
    <dbReference type="eggNOG" id="arCOG05119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1700349_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97B51"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1480.30">
        <property type="entry name" value="Designed four-helix bundle protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009205">
        <property type="entry name" value="FlaC_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05377">
        <property type="entry name" value="FlaC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="157" mass="17896" checksum="580A156D20E44953" modified="2001-10-01" version="1">MTTMGLLNNMKKLVKKEATATPQQSISEEKQVQAIHVTNGIDPKVIDAMNERMTKIENNLGKMTAELEGQKKSLSDMKNELEGIKENVKMVVSLYELVSRDFNPFMDKTPEEIRGITEELSDQINNLKRLVESAIKDLRELYGVPDIDQVIQMEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B76</accession>
    <name>Q97B76_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0570508 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0570508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916840.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B76"/>
    <dbReference type="STRING" id="273116.gene:9381368"/>
    <dbReference type="PaxDb" id="273116-14324796"/>
    <dbReference type="GeneID" id="1441688"/>
    <dbReference type="KEGG" id="tvo:TVG0570508"/>
    <dbReference type="eggNOG" id="arCOG07379">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067782_0_0_2"/>
    <dbReference type="OrthoDB" id="55602at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="13"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="277" mass="30866" checksum="953641BB4BEF7AB4" modified="2001-10-01" version="1">MYDAEISRRNPGLFLFLVDQSRSMVRKIAGGSESKAKEAADAINRQIGELIMRCTKNDGVRDYFYVGVIGYGGEKGKASYLLDDDLVPVSKLAENPIRTEKRVMKIPDGSGGTADVEYEFGVWFEAVANSDTPMVKAMNMAKEAVEKWVSEHPSSYPPIIINVTDGQSTDGDPYPVARNIMEMETDDGNPMIWNCHISSENAAPLSFPYSENQLPNDMYARSLFKMSSILPTKYINYAIEAFPDIRDGSRSYVFNAQLEQMIEFIDIGTRGAISSME</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BC3</accession>
    <name>Q97BC3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">TVG0523625</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916791.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BC3"/>
    <dbReference type="STRING" id="273116.gene:9381317"/>
    <dbReference type="PaxDb" id="273116-14324748"/>
    <dbReference type="GeneID" id="1441049"/>
    <dbReference type="KEGG" id="tvo:TVG0523625"/>
    <dbReference type="eggNOG" id="arCOG01136">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065946_3_1_2"/>
    <dbReference type="OrthoDB" id="30609at2157"/>
    <dbReference type="PhylomeDB" id="Q97BC3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000042">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF37">
        <property type="entry name" value="TRNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 16" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="17">
        <location>
            <begin position="7"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAB59675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="107" mass="11826" checksum="85B15A7DE115E225" modified="2001-10-01" version="1">MEVDINYFRTLDIRAAKVVSCEKVEKSRSLLRIVVDLGDQQKQIISSIADYYNPSDLIGKTIIILNNLKPAKFMGLESQGMLLAVDNEEGVKLLTVDGEVKPGSKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BD1</accession>
    <name>Q97BD1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0516499</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BD1"/>
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    <dbReference type="GeneID" id="1441041"/>
    <dbReference type="KEGG" id="tvo:TVG0516499"/>
    <dbReference type="eggNOG" id="arCOG01997">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_079909_1_0_2"/>
    <dbReference type="OrthoDB" id="10856at2157"/>
    <dbReference type="PhylomeDB" id="Q97BD1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00427">
        <property type="entry name" value="NAAT family transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508:SF1">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="203" mass="22188" checksum="9418E1D2D70B517B" modified="2001-10-01" version="1">MNDIVEFLKVFIPLFIVIDPFGSLVLFTSMTYNFNEGEKMRATKDAVVYGGIILVMFALAGQFIIYFFGISIEALEMAGGVILLLMAVEMIMEGNRPKTSGSEVTTDLDIGIVPFATPLLAGPGAISLVIILMKGPLIDKIFTFVSIGIIFLMVYIFFKEASIVSKFIGNKAMKAVSRIFGLLLAGFAIQYFIDAVTALFLVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BE1</accession>
    <name>Q97BE1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0503027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916773.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BE1"/>
    <dbReference type="STRING" id="273116.gene:9381298"/>
    <dbReference type="PaxDb" id="273116-14324730"/>
    <dbReference type="DNASU" id="1441031"/>
    <dbReference type="GeneID" id="1441031"/>
    <dbReference type="KEGG" id="tvo:TVG0503027"/>
    <dbReference type="eggNOG" id="arCOG00695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_0_2"/>
    <dbReference type="OrthoDB" id="372084at2157"/>
    <dbReference type="PhylomeDB" id="Q97BE1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050270">
        <property type="term" value="F:S-adenosylhomocysteine deaminase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CACAO"/>
    </dbReference>
    <dbReference type="CDD" id="cd01298">
        <property type="entry name" value="ATZ_TRZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
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        <property type="entry name" value="PRK06380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF11">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="51"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="418" mass="46102" checksum="D05EE7EEBA8B8DF6" modified="2001-10-01" version="1">MSIAISNAIIVTQNDNRDVYRGNVKIDGNKIQYVGKDEVDGDILIDGTNKVLMPGLINTHAHVGMSASKGLFDDVDLDSFLKKTFEYDSDRTSTGIFNSAKLGMYEMIGNGITAFVDLYYSEDIIAKAAEEIGIRAFLSWVTLDKELTTQIGDPVENAENFIRNHVGNKYVVPSVGVQGIYVANDDTFRRAMDLAEKYGTILHMHLSETRKEVYDAVKKLGERPIEHLHNIGILNKRVLAAHCVWATYHEVRLLSKDGVNVSWNSISNFKLGTGGIPPIPEMMRENVNVTIGTDSNGSNNSLDMFQAMKFSAISVKNYRWDASLMKAQEILDMATRNAARALQLNAGSIEVGKLADVVLLDATIPSMIPTNEANAVSNIVYSSSPQNVDTVIIDGEIKKINGKILGFHSNKFTNSEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BF4</accession>
    <name>Q97BF4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0489921 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0489921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916760.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BF4"/>
    <dbReference type="SMR" id="Q97BF4"/>
    <dbReference type="STRING" id="273116.gene:9381285"/>
    <dbReference type="PaxDb" id="273116-14324717"/>
    <dbReference type="DNASU" id="1441018"/>
    <dbReference type="GeneID" id="1441018"/>
    <dbReference type="KEGG" id="tvo:TVG0489921"/>
    <dbReference type="eggNOG" id="arCOG00449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029027_0_0_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="14"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="272" mass="29782" checksum="847372BC48DE26DB" modified="2001-10-01" version="1">MFEIPRPYSLHLGKIAVPMAEGRSSRRVLHIAMALADVFGSEISAVTVRDQAKDIMWTNKINVVTNAYKEGLARNIKIIPKIITSSDVKSALINEINRHSYDLVIIASEKRSIITRITSGPVSNQVIKKAESPTALVSVKTQNFPYKSIYIPLSETINTRSAVAFGLTLKKVMGAKAVLADLRAFDSNPTHRFSYIFDYMNEIVSNFGDNVTVIKGGTKYDLKESVLNGAASTGADAVVLGVKAGENGKIRVNSIIKDLMKTGDFDSILFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BG6</accession>
    <name>Q97BG6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0475190</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916748.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BG6"/>
    <dbReference type="STRING" id="273116.gene:9381271"/>
    <dbReference type="PaxDb" id="273116-14324704"/>
    <dbReference type="GeneID" id="1441006"/>
    <dbReference type="KEGG" id="tvo:TVG0475190"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_60_4_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="PhylomeDB" id="Q97BG6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17329">
        <property type="entry name" value="MFS_MdtH_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="13"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="410" mass="46294" checksum="B8C2CED2921CFAFC" modified="2001-10-01" version="1">MAYIYGFRTLDRRVFYLALSRLIRASGRVSSFIFLPLIFIEIYSISFLITGLILGSSAIAMAFVQYYSGRWTDRIGRRFFLIVIPIPAAVFYFLMFTTVYLHMSVILLIIFWYGTIITNSLQYPAIEASVADVTSTSERLSGYTLIRIMANLGAAVGPLAGGFLAGINFSYIFLLASIFTVIELFILYYNVRETFLPARAIAEKIKLKIFKTDRFFFLFTLVGVFLGFTLRQNGPTLTVYAFDLKNLPLMDIGYLYSLNGIVVIALQMPILRLMSSRSNPVFWRGIGTIYYSIGFVILGIAPTFLYILLAMFIFTVGEDFMSPTTQTIITTLAPAEKKGSYIGSYNLLTSFGRFFGTIFGLYFLFIYQKTTFTFWIIIATSTGVIGLLYIFMSESFIKRTSKIATLPGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BI4</accession>
    <name>Q97BI4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavoprotein reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0461107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916731.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BI4"/>
    <dbReference type="STRING" id="273116.gene:9381253"/>
    <dbReference type="PaxDb" id="273116-14324686"/>
    <dbReference type="DNASU" id="1440989"/>
    <dbReference type="GeneID" id="1440989"/>
    <dbReference type="KEGG" id="tvo:TVG0461107"/>
    <dbReference type="eggNOG" id="arCOG01064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_5_1_2"/>
    <dbReference type="OrthoDB" id="38899at2157"/>
    <dbReference type="PhylomeDB" id="Q97BI4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="388" mass="43223" checksum="843BFD7D9610BB63" modified="2001-10-01" version="1">MAKKIVIIGAGAGGGISLSKLRRVLKDEDVDITIIDKLGRTDFQPSYIFLSIGQKRVKDLSRDFNLLNGGHVKTVKDEVVSVDAQNRIVNTLKGKYSYDYLIMSPGPDIKKDSFEGIEATDSVWTLEDSLKLRAKIKEFNGGKILISVSSQWYKCPPVPWEMALLLDDYFRKKNMRDKVEITVAHPVSKPMEMYGPGLSDPFAKMLEKRSIGGLYGKKLLGIDASKKEAVFEDDDRLRFDLSIVAPPHSPPSFIKDNEYLRSKNGWASTNLKDFRNPKYDDIFAIGDVIAPTIQIGMAGVLAHFQADTASSAIAEDIIGYPFIDYNKNAVCIIATGSSGVFSYCDLSAKLTDPSVKFPECRMIGNDPLFRFAHDLYEVNFLSSIYGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BJ2</accession>
    <name>Q97BJ2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type I restriction modification DNA specificity domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0452430</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916722.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BJ2"/>
    <dbReference type="PaxDb" id="273116-14324678"/>
    <dbReference type="GeneID" id="95969395"/>
    <dbReference type="KEGG" id="tvo:TVG0452430"/>
    <dbReference type="eggNOG" id="arCOG02626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718319_0_0_2"/>
    <dbReference type="OrthoDB" id="214860at2157"/>
    <dbReference type="PhylomeDB" id="Q97BJ2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1120">
        <property type="entry name" value="Bipartite methylase S protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="entry name" value="DNA methylase specificity domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052021">
        <property type="entry name" value="Type-I_RS_S_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30408:SF12">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME MJAVIII SPECIFICITY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30408">
        <property type="entry name" value="TYPE-1 RESTRICTION ENZYME ECOKI SPECIFICITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116734">
        <property type="entry name" value="DNA methylase specificity domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Type I restriction modification DNA specificity" evidence="4">
        <location>
            <begin position="9"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="152" mass="17405" checksum="F99D36D228CE5C40" modified="2001-10-01" version="1">MIALNGQGKTKGMVGILKVESTCNQSLAAFNVNERTLHYRYLYYFLKSKYKQMRGLVGDDLRDGLSLSVLRELRIPVPSLQEQFAISNYSDNQIHVIKNMISKQEKMIELLKEHRASLITPRSNRQDRCKGISQGGERICQTNSMKLILKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BM6</accession>
    <name>Q97BM6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG0419177 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0419177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916687.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BM6"/>
    <dbReference type="STRING" id="273116.gene:9381208"/>
    <dbReference type="PaxDb" id="273116-14324644"/>
    <dbReference type="GeneID" id="1440945"/>
    <dbReference type="KEGG" id="tvo:TVG0419177"/>
    <dbReference type="eggNOG" id="arCOG03287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026835_0_0_2"/>
    <dbReference type="OrthoDB" id="8543at2157"/>
    <dbReference type="PhylomeDB" id="Q97BM6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004135">
        <property type="term" value="F:amylo-alpha-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010401">
        <property type="entry name" value="AGL/Gdb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032790">
        <property type="entry name" value="GDE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006451">
        <property type="entry name" value="Glycogen_debranch_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024742">
        <property type="entry name" value="Glycogen_debranch_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01561">
        <property type="entry name" value="gde_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569:SF2">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06202">
        <property type="entry name" value="GDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12439">
        <property type="entry name" value="GDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycogen debranching enzyme bacterial and archaeal type N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme C-terminal" evidence="1">
        <location>
            <begin position="261"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="601" mass="69461" checksum="D9D5F3848B02AA91" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BR8</accession>
    <name>Q97BR8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0374267 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0374267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916641.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BR8"/>
    <dbReference type="STRING" id="273116.gene:9381164"/>
    <dbReference type="PaxDb" id="273116-14324602"/>
    <dbReference type="GeneID" id="1440899"/>
    <dbReference type="KEGG" id="tvo:TVG0374267"/>
    <dbReference type="eggNOG" id="arCOG07402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1297536_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="212" mass="24182" checksum="EAAAD412DB6D8D70" modified="2001-10-01" version="1">MVFFKFGKSEEEKMLERRSRISKWKSDIDRAIRQYEKNREMNIQNAKISLRDGNLEKARVFASNIVSLESAIRGLKDYKLFLENIDLNLQFADTTKKVWASLKEGSEDLLKSQLTEKQVIQMQQNVEKIINSSDQIQERLSSQLDQITTAVNQRGEYNVESVDKILETLKGDTEASKSASRTSEPAQGEATTDKELEDLLKSLGGVKSDDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BS1</accession>
    <name>Q97BS1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0371051 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0371051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916638.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BS1"/>
    <dbReference type="STRING" id="273116.gene:9381161"/>
    <dbReference type="PaxDb" id="273116-14324599"/>
    <dbReference type="GeneID" id="1440896"/>
    <dbReference type="KEGG" id="tvo:TVG0371051"/>
    <dbReference type="eggNOG" id="arCOG07404">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329538_0_0_2"/>
    <dbReference type="OrthoDB" id="57208at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="206" mass="24191" checksum="2D28E08D1D58A352" modified="2001-10-01" version="1">MGKYGMIVFTDSNLKTDLLLFRNHAEMLVPPRKYFNEFILYSEGGNTHFYIGSSSNGAYELLRSFFTRLYPCYVKESNPSIKRGRGRIYYVSREKKKSFEYYFPDLLNSIATLAAMFPECNIAYLCRVYSSLSLSGRKYGLFIRISSCEEYNKEIMEFLDQQIRSLKKRHGIKMKRCRFVIPLRKNSMSMAFNLASFIRIPEDVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BU7</accession>
    <name>Q97BU7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="7">ATase</shortName>
            <ecNumber evidence="7">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="7">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purF</name>
        <name evidence="14" type="ORF">TVG0348801</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7 10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7 11">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="7"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BU7"/>
    <dbReference type="STRING" id="273116.gene:9381135"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="PaxDb" id="273116-14324573"/>
    <dbReference type="KEGG" id="tvo:TVG0348801"/>
    <dbReference type="eggNOG" id="arCOG00093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_2"/>
    <dbReference type="PhylomeDB" id="Q97BU7"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 7 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="13">
        <location>
            <begin position="35"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7 9">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB59500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="489" mass="53247" checksum="877928C48763DFE9" modified="2001-10-01" version="1">MDTTPITSGLRGAPTVDSRPQGSDPINGQKPSEDCAVVGFKGKINAYNPIITALRTLQHRGQESAGMAVFDGKKVTLKKGSGLVTDVFNPATDDIKGYVGVGHTRYSTAGSKNVVNAGPFVMNSSFGYITISHNGEIVNADELRDSMKKEGITFQSDSDTEVMLAELSRNISKYGLKRGFEQSMESLRGAYACAISINDRLYAVRDPNGIRPLVIGKNNDGYIVASESCAIDALEGTLIKNIEPGEVVEISDEGIRTIVSKSANRIAHCMFEYVYFSRPDSVIDGINVYAARVNMGRILAKESPVEADIVVPVPDSGRSQAIGYSMESGMPYTEGLIKNRYSERTFIMPTQSDRKAAIHLKLNPIREVIGGKKVVLVDDSIVRGNTMRFIVGLMRKYGAKEIHVRIGSPHIIAPCYFGVDMKTKDQFIARGKTDEEINREIGADSLAFLSVDGLKQAISMKNNNLCLGCLTGEYPIDISKKLAENITSY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C14</accession>
    <name>GCSH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvH</name>
        <name type="ordered locus">TV0291</name>
        <name type="ORF">TVG0303364</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="1">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916546.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C14"/>
    <dbReference type="SMR" id="Q97C14"/>
    <dbReference type="STRING" id="273116.gene:9381065"/>
    <dbReference type="PaxDb" id="273116-14324506"/>
    <dbReference type="GeneID" id="1440804"/>
    <dbReference type="KEGG" id="tvo:TVG0303364"/>
    <dbReference type="eggNOG" id="arCOG01303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_2_2"/>
    <dbReference type="OrthoDB" id="9810at2157"/>
    <dbReference type="PhylomeDB" id="Q97C14"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0450">Lipoyl</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000166289" description="Probable glycine cleavage system H protein">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="23"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01066"/>
        </source>
    </evidence>
    <sequence length="122" mass="13883" checksum="F5F1BAECEF837114" modified="2001-10-01" version="1">MTNVPDGLKYTKTHEWYKVDNGIATVGITDYAQSQMTDIVYVDLPEVGDKKKVGDVLLTIESVKSAEDVYSPLTGEITEVNQELTKHPENINKDPYGNWLVKMKVEKEGEYLTAEQYRKLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C34</accession>
    <name>Q97C34_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0282227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916528.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C34"/>
    <dbReference type="STRING" id="273116.gene:9381044"/>
    <dbReference type="PaxDb" id="273116-14324485"/>
    <dbReference type="GeneID" id="1440786"/>
    <dbReference type="KEGG" id="tvo:TVG0282227"/>
    <dbReference type="eggNOG" id="arCOG00910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074702_1_0_2"/>
    <dbReference type="OrthoDB" id="31271at2157"/>
    <dbReference type="PhylomeDB" id="Q97C34"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22234" checksum="87A0E7172B731D3F" modified="2001-10-01" version="1">MGIKSALEIQLQKLKQPEHYANYLEQYMTDASSAAYFLVEILEDGNIKGRTVVDAGTGNGILACGAYYLGAATVLGFDIDVSMVEIAKQNCNNVKFEVRNVKDISGKFDTWIMNPPFGSVMKHADRPFIEKAFETSKFIYSIGNAKAESFLSREFSARGDIFREEHIDLSVPRIYEHHKKDWTRIPAVIFGVKNYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C41</accession>
    <name>Q97C41_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Farnesyl pyrophosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0276764</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916520.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C41"/>
    <dbReference type="STRING" id="273116.gene:9381037"/>
    <dbReference type="PaxDb" id="273116-14324478"/>
    <dbReference type="GeneID" id="1440778"/>
    <dbReference type="KEGG" id="tvo:TVG0276764"/>
    <dbReference type="eggNOG" id="arCOG01726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_1_2"/>
    <dbReference type="OrthoDB" id="26738at2157"/>
    <dbReference type="PhylomeDB" id="Q97C41"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43281">
        <property type="entry name" value="FARNESYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43281:SF1">
        <property type="entry name" value="FARNESYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="354" mass="40091" checksum="D1788EA6003E30B3" modified="2001-10-01" version="1">MQIESQSPTDVVASYKAKIDESISRFFDRKEEETKDVLSKRVIKIIRDYTEGGGKRLRPIFMILGYRLFSPEEKGIFDASISIELAQSYLLIHDDVIDKSDVRRGKPSMHIRLEDAFVAGCPQSRSTAEGLAIVAGDLAESYAHEALLRSGFYSENLVKADLELSKTIEMTGYGQFLDILSTTMDDFNEGDLIRLHLWKTAKYTLEGPLAMGAILSGTEYDITALREYGRVLGIAFQLKDDILGLFGDEKTIGKSVYSDVNEGKKTLLMIKAMEWGDRDDANFIRKMLRKGNVTNEEFERIKKLVMDTGSYDYSINLMGKLVDKSKEYLAKVSGDNYVKKILDWLSDYLISRDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C47</accession>
    <name>Q97C47_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0270675</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916515.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C47"/>
    <dbReference type="STRING" id="273116.gene:9381030"/>
    <dbReference type="PaxDb" id="273116-14324472"/>
    <dbReference type="GeneID" id="1440773"/>
    <dbReference type="KEGG" id="tvo:TVG0270675"/>
    <dbReference type="eggNOG" id="arCOG01991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_9_3_2"/>
    <dbReference type="OrthoDB" id="304253at2157"/>
    <dbReference type="PhylomeDB" id="Q97C47"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054929">
        <property type="entry name" value="dPGM_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038349">
        <property type="entry name" value="dPGM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="201" mass="22389" checksum="2119DE62F580801A" modified="2001-10-01" version="1">MKIAILIRHGESDINVKGILSDSIDGNSLTERGIRQAEHARDQLTGLEIKNVYSSPINRARETAEIIASSFGKEVVIDDRLREIGLGKASGRRASDFRDELYTGHISGSIRKELEMEEWTSLQTRVVEALAAREGINVYTTHSDPIRAAISYFLELGEPESYGISIKNASMTFIDVEIGRILSIGSIIVSDEIKKYVSRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C57</accession>
    <name>Q97C57_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cas12f1-like TNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0262802</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760299.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C57"/>
    <dbReference type="STRING" id="273116.gene:9381020"/>
    <dbReference type="PaxDb" id="273116-14324462"/>
    <dbReference type="GeneID" id="1440764"/>
    <dbReference type="KEGG" id="tvo:TVG0262802"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_791354_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97C57"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="3">
        <location>
            <begin position="281"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="386" mass="44554" checksum="1F78D0E0C061463C" modified="2001-10-01" version="1">MYAFKAVKQYFYPSDNLKVLMHKFTDMVNFTINIMIEKNLTSRNSVSNEVYHKLKEYEIPSYYYIEAINKAVALVKTYRKRLKKKQKATVPHVEKPMLSTYYGFKIVGGNLMIPIANREYESIPLNAYVRDATSMVKVHSFALSAYTLSLTIGRDVDAIECTTTVGVDRNLRNATVGNEFHHEIYDLSNVVEIKQRYRKKESHFHRNDRRIREKILSKYGKRERNRVKQIMHETSKAIVANASTRKEMIVLENIRGINKITKKGDGKGKDYRFLLKNAFPYGMLASQVMYKASWEGLPVIELTRKETRNTSRMCSVCDTLARIEHGRILKCDSCGLEIDRDVNACINIAFRYRTCLKRSHKGLPGEAVKQSKDVEQMAGSQISTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C76</accession>
    <name>Q97C76_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG0233936 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0233936</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916482.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C76"/>
    <dbReference type="STRING" id="273116.gene:9380998"/>
    <dbReference type="PaxDb" id="273116-14324441"/>
    <dbReference type="GeneID" id="1440742"/>
    <dbReference type="KEGG" id="tvo:TVG0233936"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051127_0_0_2"/>
    <dbReference type="OrthoDB" id="86286at2157"/>
    <dbReference type="PhylomeDB" id="Q97C76"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="230"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="430" mass="47118" checksum="3B2D9D1922874CC5" modified="2001-10-01" version="1">MPSNKWFISYLMSSMASGLTNPMIPLFVVVYLHSNVFFVGVASAVSSAASVPALIFWGNLSDSLSRRKVFILIGFFGAFASFLPIFFVRNIYEYMAILILFQVLAMASVPVSTVMILEQNDRSQWPQVISNFSMISGVGSVIGLVIGTVFITLEYSPSALPLVYILSSIIYLIAGVTAIKLISEPGRHITRNDVPLHTFRVVERARYFPTSVIHFLSLSSGKNGENLDKNVFLFITMTAFLMFGFQLFFVPYPVMVIKFGGNNILIFVMYALNSLLSTISFMFTGRIVTRLGGKTSLFIAVFSRVIIFSAAAAIPMFLYGTYALVSAVAVYAVLGGIWSIISVSQTNYISRNSGQKARGKAIGLYNSLLGVGQILGGLTSGIITTLYGYSIDYITSALIIMAGLVIMMKIYRGEKIFSFSKASPDKYGQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C80</accession>
    <name>Q97C80_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Thioredoxin peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0230902</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916478.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C80"/>
    <dbReference type="STRING" id="273116.gene:9380994"/>
    <dbReference type="PaxDb" id="273116-14324437"/>
    <dbReference type="GeneID" id="1440738"/>
    <dbReference type="KEGG" id="tvo:TVG0230902"/>
    <dbReference type="eggNOG" id="arCOG00312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_21_3_2"/>
    <dbReference type="OrthoDB" id="145578at2157"/>
    <dbReference type="PhylomeDB" id="Q97C80"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03015">
        <property type="entry name" value="PRX_Typ2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000002">
        <property type="entry name" value="Alkyl hydroperoxide reductase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF121">
        <property type="entry name" value="ALKYL HYDROPEROXIDE REDUCTASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 10" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="2"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="8">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="174" mass="19755" checksum="9A8D9346EB839742" modified="2001-10-01" version="1">MSLVNKAAPDFEANAFVNGEVKKIRLSSYRGKWVVLFFYPADFTFVCPTEVEGFAEDYEKFKKKNTEVISVSEDTVYVHKAWVQYDERVAKAKYPMVEDRKGIIARAYDVYNEETGNAQRGLFIINPDGIVKYVVITDDNVGRSTDETLRVLEALQSGGLCPVNWHEGEPTLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C81</accession>
    <name>Q97C81_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Signal sequence peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0230905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C81"/>
    <dbReference type="STRING" id="273116.gene:9380993"/>
    <dbReference type="PaxDb" id="273116-14324436"/>
    <dbReference type="KEGG" id="tvo:TVG0230905"/>
    <dbReference type="eggNOG" id="arCOG01739">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054902_2_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06462">
        <property type="entry name" value="Peptidase_S24_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="251" mass="28263" checksum="CF85CD13DA8AC83B" modified="2001-10-01" version="1">MAIIIFVLAATIYGGIWPPFSVVESESMEHGSSWEPGVINTGDIVLLKKVNDPLNNIVTYVDGRSIGFKTYGEYGDVILYNAPDGEIIIHRAMFYLTWDNGNPVVYGYHGQSWIKVTKEYVIIYNCSYNGRNLFVSLKGMENESGFITVGDHNLATSKEFLSQYDAYIAADQNIFGFPPVPPSKVVAVAYGQIPWLGLIKLNIMRLYGEWPYYNEVPKNAYTYLLSLIASIIIIPYASINIYKKIKKKVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C91</accession>
    <name>Q97C91_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pirin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0220708</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916468.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C91"/>
    <dbReference type="STRING" id="273116.gene:9380982"/>
    <dbReference type="PaxDb" id="273116-14324426"/>
    <dbReference type="GeneID" id="1440728"/>
    <dbReference type="KEGG" id="tvo:TVG0220708"/>
    <dbReference type="eggNOG" id="arCOG02935">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045717_5_0_2"/>
    <dbReference type="OrthoDB" id="23530at2157"/>
    <dbReference type="PhylomeDB" id="Q97C91"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="3">
        <location>
            <begin position="21"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="4">
        <location>
            <begin position="195"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="34067" checksum="82CEF0F635DBB896" modified="2001-10-01" version="1">MDIKIPELIIEGVYTSDGAGVKLERIFGSPRTVQITDPFLLLDFFGSSKLSDYENGFPWHPHRGIETITLQLRGKTYHEDSEGHKGIILPGELQWMTAGSGIFHQEMPKPIYYGDKVPSIKDDANAGIQLWLNMPAANKMDEPAYRSIREEQVPVEEDDYGNKIGVISGNFKKVAGALNESFQYELAEKINPYYLRIKTIAKGKFETNIPEGHRAIIFVIGGLISVNDKELVSEGRAVILSTKGKLLSFYADQPSDVIIIAGRPLNEPISWYGPIVMNTREEIAQAIKELNDGTFIKENNPLWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CA7</accession>
    <name>Q97CA7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Isocitrate dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="4 13">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">TVG0201218</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="20 21" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="16">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="4 13"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916451.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CA7"/>
    <dbReference type="STRING" id="273116.gene:9380965"/>
    <dbReference type="PaxDb" id="273116-14324409"/>
    <dbReference type="GeneID" id="1441681"/>
    <dbReference type="KEGG" id="tvo:TVG0201218"/>
    <dbReference type="eggNOG" id="arCOG01164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_7_1_2"/>
    <dbReference type="OrthoDB" id="23624at2157"/>
    <dbReference type="PhylomeDB" id="Q97CA7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004439">
        <property type="entry name" value="Isocitrate_DH_NADP_dimer_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005036">
        <property type="entry name" value="PRK06451.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005425">
        <property type="entry name" value="PRK07006.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00183">
        <property type="entry name" value="prok_nadp_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43504">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43504:SF1">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="8 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 16" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="19">
        <location>
            <begin position="19"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="324"/>
            <end position="330"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="17">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="17">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-succinyllysine" evidence="18">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="18">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR00183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BAB59337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="405" mass="45192" checksum="59719F5D3991B71F" modified="2001-10-01" version="1">MAYIQVREDGTLLVPDKVTIGYIEGDGIGPDITKTTINVINAALEVAYGGKRSIEWHKVLAGDEAYDKTGNRLPDSSLEELKKCVVSIKGPLTTPIGEGFRSLNVTLRQYLDLYANIRPVRYFPGVPSPVLHPEYMNMVVFRENTEDLYAGIEWRYDSEDAEKVRAFLSSTFNINLTPDTGIGIKPISKFKSTRLVRKAIQYAIANGRKSVTLVHKGNIMKYTEGAFRDWGYEVARTEFGDYTVTEAEYLKDPEKYANKIVIKDRITDNMFQQVLTRTQEYDVIATPNLNGDYLSDALAAQVGGIGLAPGANIGDHYAVFEAVHGTAPKYAGLDVANPTSLILSAEMMLDHIGWKEASKILDDAIMQTYRDQKLTQDIARLLNLKALKCSEFGEEIINRMKPIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CB0</accession>
    <name>Q97CB0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0198521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CB0"/>
    <dbReference type="STRING" id="273116.gene:9380962"/>
    <dbReference type="PaxDb" id="273116-14324406"/>
    <dbReference type="KEGG" id="tvo:TVG0198521"/>
    <dbReference type="eggNOG" id="arCOG05349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691464_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="176" mass="20798" checksum="3D0AC3C9F1E8D483" modified="2001-10-01" version="1">MIHCDPFSINCFWENVIKFLQYDHSMFKIAITSVPARVDVSDLDRSLAYFLSDIGYIPKINPSTDMNSIKNSVYFRLFKECFLMNGERFWTPEELMSYLGTSRSTLYRHLNKLKYMDLLEEVKDGKIKKYRIRSGDILKAWSWVEINVKMAMDNYRSNVEHIVDLIRSNSNSNLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CB1</accession>
    <name>Q97CB1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0197945</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916447.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CB1"/>
    <dbReference type="STRING" id="273116.gene:9380961"/>
    <dbReference type="PaxDb" id="273116-14324405"/>
    <dbReference type="GeneID" id="1441677"/>
    <dbReference type="KEGG" id="tvo:TVG0197945"/>
    <dbReference type="eggNOG" id="arCOG00109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_6_2_2"/>
    <dbReference type="OrthoDB" id="27149at2157"/>
    <dbReference type="PhylomeDB" id="Q97CB1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011529">
        <property type="entry name" value="PRK14968.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="4">
        <location>
            <begin position="27"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="183" mass="20932" checksum="6BC13E190BAB9AB0" modified="2001-10-01" version="1">MKEYFGIRIEECESVYEPSDDTFLLMQYAECKGRAIEIGCGTGLVSIYFKKRGCNIECVDLNQSAVDCTKRNAEINGVSLNVYASDLFEAARGVYDTVLFNAPYLPVSDEDMAWSGGKSMELISRFLRESKNHINRSSNIFIVLTDLTDNESLFMDEGFLYEVIKKYCFDFEDILLYKLNMKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-28" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CB4</accession>
    <name>PTH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="1">PTH</shortName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pth</name>
        <name type="ordered locus">TV0188</name>
        <name type="ORF">TVG0193013</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916443.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CB4"/>
    <dbReference type="SMR" id="Q97CB4"/>
    <dbReference type="STRING" id="273116.gene:9380958"/>
    <dbReference type="PaxDb" id="273116-14324402"/>
    <dbReference type="GeneID" id="1441673"/>
    <dbReference type="KEGG" id="tvo:TVG0193013"/>
    <dbReference type="eggNOG" id="arCOG04228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073661_2_2_2"/>
    <dbReference type="OrthoDB" id="6075at2157"/>
    <dbReference type="PhylomeDB" id="Q97CB4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00628">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034759">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003314">
        <property type="entry name" value="PRK04322.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000120307" description="Peptidyl-tRNA hydrolase">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00628"/>
        </source>
    </evidence>
    <sequence length="117" mass="13000" checksum="7404C57601BACE92" modified="2001-10-01" version="1">MVVKLVVAVRKDLDMGKGKIAAQVAHAAVSCAIKAMKEKKKIFDEWMDEGQKKIVVKVPNVDEIYIIKKKADSMGIINEVIQDRGYTQVEPGTVTCIGLGPDYEVYLDDITGKYKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CF8</accession>
    <name>Q97CF8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0148825 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0148825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CF8"/>
    <dbReference type="PaxDb" id="273116-14324357"/>
    <dbReference type="KEGG" id="tvo:TVG0148825"/>
    <dbReference type="eggNOG" id="arCOG07762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631545_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CF8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="170" mass="19694" checksum="D38DDF58CA97E996" modified="2001-10-01" version="1">MLEASSRIREDEFGRPSVIHEVDRAQIILSKEMFDLSNRKAAYLMPLMGMEYEVSYKTVERIYSDDVVPMIMHNHFVLSVKKRGIDISNACRDRTGYSLTVSDHHRSVREKLGESVKHGKYRYSFAIMDLKTRMYIGYASFVRSESDAYQKASRIISDLGIQINSVRLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CH2</accession>
    <name>Q97CH2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="19">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="19">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="19">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="19">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="17" type="primary">nnrD</name>
        <name evidence="18" type="synonym">nnrE</name>
        <name evidence="22" type="ORF">TVG0137051</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="22 23" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 19">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18 19">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 18 19">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17 19">
            <text>(6S)-NADHX + ADP = AMP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17 19">
            <text>(6S)-NADPHX + ADP = AMP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="18 19">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="19"/>
    <dbReference type="EC" id="5.1.99.6" evidence="19"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CH2"/>
    <dbReference type="STRING" id="273116.gene:9380898"/>
    <dbReference type="PaxDb" id="273116-14324343"/>
    <dbReference type="KEGG" id="tvo:TVG0137051"/>
    <dbReference type="eggNOG" id="arCOG00018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_4_1_2"/>
    <dbReference type="PhylomeDB" id="Q97CH2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12 17 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 17 19" id="KW-0520">NAD</keyword>
    <keyword evidence="8 17 19" id="KW-0521">NADP</keyword>
    <keyword evidence="6 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 18 19" id="KW-0630">Potassium</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="21">
        <location>
            <begin position="27"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF C-terminal" evidence="20">
        <location>
            <begin position="215"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="137"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR017184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BAB59271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="480" mass="52076" checksum="9440E8C91927E82B" modified="2001-10-01" version="1">MNRMQKIFSLTISFKLSCLGMGYYDDMRHDDLNYEYLYGSTYPLMKNAGSSVAKVIEKNFASGQKVCVVCGTGNNAGDAIVAAELLRTKFEVTVLLVNGLKSELAKKALQEYGGNVVDASHIDECLAADIIIDGIFGVGISGEPKEPYKSTIEKINASGKKIVSVDVPSGLGTNCAVRPDITVTFTSLKYGMNKENSGEIIVADIGIPDDVRKYAGPGDLIYYPRPDPDSHKGMNGTLGIVAGWEYHGSSVIAALAAENLGLDLVRIYVTNKNYEIVSSYDPGIIVRLFDYKRQASYEEVWRNSALLIGPGLGTSHEAEIALKRLVNGSAVPIVIDADGITLLSKHLSIIKGKKIVVTPHKNEFRKLTGTEPNEENAVEFAKEKGIIIVLKGKVDIITDGKEVHYAKGGNARMTMGGTGDLLAGLISSFISKGIDPMRSCLMGTYLNKRIGELAYDKKGLFYKITDMIDEIPSVMASYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CH6</accession>
    <name>Q97CH6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0132669 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0132669</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CH6"/>
    <dbReference type="STRING" id="273116.gene:9380893"/>
    <dbReference type="PaxDb" id="273116-14324339"/>
    <dbReference type="KEGG" id="tvo:TVG0132669"/>
    <dbReference type="HOGENOM" id="CLU_3094335_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="51" mass="5749" checksum="6D499E3813144579" modified="2001-10-01" version="1">MSISLVAKFRIICTDGEKLLILAENIIIQFLNFTTQSKKYLECGDLGVKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CH8</accession>
    <name>Q97CH8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acetyl-CoA synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0130538</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CH8"/>
    <dbReference type="STRING" id="273116.gene:9380891"/>
    <dbReference type="PaxDb" id="273116-14324337"/>
    <dbReference type="KEGG" id="tvo:TVG0130538"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_2"/>
    <dbReference type="PhylomeDB" id="Q97CH8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015645">
        <property type="term" value="F:fatty acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A synthetase ACSM5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051087">
        <property type="entry name" value="Mitochondrial_ACSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605:SF10">
        <property type="entry name" value="ACYL-COA SYNTHETASE MEDIUM CHAIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="20"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="417"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="60467" checksum="989FD84D49C9BB78" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CI9</accession>
    <name>Q97CI9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRISPR associated protein Cas6 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0118145</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916369.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CI9"/>
    <dbReference type="STRING" id="273116.gene:9380880"/>
    <dbReference type="PaxDb" id="273116-14324326"/>
    <dbReference type="GeneID" id="1441599"/>
    <dbReference type="KEGG" id="tvo:TVG0118145"/>
    <dbReference type="eggNOG" id="arCOG04342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089858_1_0_2"/>
    <dbReference type="OrthoDB" id="43942at2157"/>
    <dbReference type="PhylomeDB" id="Q97CI9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21140">
        <property type="entry name" value="Cas6_I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049435">
        <property type="entry name" value="Cas_Cas6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045747">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984:SF1">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01881">
        <property type="entry name" value="Cas_Cas6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRISPR associated protein Cas6 C-terminal" evidence="4">
        <location>
            <begin position="110"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="237" mass="28043" checksum="E8CCA7AEBE74F7E7" modified="2001-10-01" version="1">MRLKVTFYTKDDENYEFNKYDIQGMIYSALLDAGMTEIHAGNKIRFFCFSDIFPSNILKKDFLFNFIISSPDSNIIKNIYNILDKNKIFYLSGYKFHIAEIKEFDINISKDFITGSPVVLYLDNKKNKYFSIKDDSIAFFLRRLKENAIKKFNLYYHENININHLIFDKLKFHREVALLLRKGKTSFNIIGTTWYNLHLNKLNRDEIKFYNFIMDAGIGEKNSLGFGFLNPVRNYGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CJ9</accession>
    <name>Q97CJ9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Methionine aminopeptidase</fullName>
            <shortName evidence="8">MAP</shortName>
            <shortName evidence="8">MetAP</shortName>
            <ecNumber evidence="8 9">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">map</name>
        <name evidence="11" type="ORF">TVG0107310</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="8 9"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916359.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CJ9"/>
    <dbReference type="STRING" id="273116.gene:9380870"/>
    <dbReference type="PaxDb" id="273116-14324316"/>
    <dbReference type="GeneID" id="1441589"/>
    <dbReference type="KEGG" id="tvo:TVG0107310"/>
    <dbReference type="eggNOG" id="arCOG01001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_7_0_2"/>
    <dbReference type="OrthoDB" id="372008at2157"/>
    <dbReference type="PhylomeDB" id="Q97CJ9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="10">
        <location>
            <begin position="9"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="291" mass="32085" checksum="2948781BDFFF38EC" modified="2001-10-01" version="1">MDEDVKRKYIEAGRIGREALELARTLIEPGTKLLDVAEAMEKFVIEQGAKPAFPVNLSINNDAAHYTPASGDKKVFKTGDVVKVDFGAHIDGYMSDTAITLEVGESGKHSDLIDATREALDAAISLVRPMRSVNEIGGRIADVIGSFGFKPVRNLGGHGVERYDLHATLFIPNYDDGNVVRLQPDHAIAIEPFASTGIGMIHSGPPGNIYILDAPKVREDEVIYKNFRTLPFAERWLAGLVEDHKTYLKSMVSSREVSPFPILREHNNTLISQAEHTILVLNDEIIVTTRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CM5</accession>
    <name>DTDA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="1">3.1.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>D-tyrosyl-tRNA(Tyr) deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dtdA</name>
        <name type="ordered locus">TV0076</name>
        <name type="ORF">TVG0076358</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtdA deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916333.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CM5"/>
    <dbReference type="SMR" id="Q97CM5"/>
    <dbReference type="STRING" id="273116.gene:9380842"/>
    <dbReference type="PaxDb" id="273116-14324290"/>
    <dbReference type="GeneID" id="1441563"/>
    <dbReference type="KEGG" id="tvo:TVG0076358"/>
    <dbReference type="eggNOG" id="arCOG01616">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056464_1_0_2"/>
    <dbReference type="OrthoDB" id="9863at2157"/>
    <dbReference type="PhylomeDB" id="Q97CM5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10700">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.50">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00562">
        <property type="entry name" value="Deacylase_DtdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018033">
        <property type="entry name" value="Deacylase_DtdA_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007508">
        <property type="entry name" value="DtdA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003072">
        <property type="entry name" value="PRK03995.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667:SF1">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04414">
        <property type="entry name" value="tRNA_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016210">
        <property type="entry name" value="UCP016210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142535">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000158977" description="D-aminoacyl-tRNA deacylase">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00562"/>
        </source>
    </evidence>
    <sequence length="256" mass="28967" checksum="E1D9920D23B4A1EE" modified="2001-10-01" version="1">MKVLIASKSDPASMQMLSYLEDNYDIKENSGRRFVKDFEIFVIEDRHIFHDMNLGNNYEYAVVLSRHSSAADIKSLTAHPTGNFGPKADLGGRPKTINVSCPKYMSGTLRQMLESYSGTKFQVTFEATHHGPIFDLPNYYVEIGTTENEWTDDDAKKTAVDAVINPDAKDFPNFVAVGGGHYAPKILEYFRRNEINIGHIISKHDHDDLEEWQIKDAVEKTPSCKGFLVDRKGTRSRVRDMVKSISDDLGLELIMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CN1</accession>
    <name>Q97CN1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Threonine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0071521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916327.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CN1"/>
    <dbReference type="STRING" id="273116.gene:9380836"/>
    <dbReference type="PaxDb" id="273116-14324284"/>
    <dbReference type="GeneID" id="1441557"/>
    <dbReference type="KEGG" id="tvo:TVG0071521"/>
    <dbReference type="eggNOG" id="arCOG01434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_4_1_2"/>
    <dbReference type="OrthoDB" id="6371at2157"/>
    <dbReference type="PhylomeDB" id="Q97CN1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004996">
        <property type="entry name" value="PRK06381.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="1">
        <location>
            <begin position="11"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="316" mass="34996" checksum="26B7030C0EE18E73" modified="2001-10-01" version="1">MEELLNSETKPPGRTPTIKAYALGKELRLNNLFLKFEGANATGTQKDRISEAHVLNAIRDGYNGIAVGTCGNYGASIAYYANLYGIKSYIGMPSAYTHDREDFMLSSNAEILYYNGKYEETVSFIHDYATDNSLYDASPGSDHSYIDTKMYAGIAFEIYEAILKVPDYVIVPVGNGTTLSGIYEGFHLMYLNGTIDRMPRMVGVSTRGGNPIVSSWKRGYKKVVDLDPARIRETAVNEPLVSYRSFDGDKALAAIRETHGKALYVSDDEMVRFSELMYREEGLNPLPASASAVAALVHLHLNPDDYVVSVITGRRM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-07-05" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CP5</accession>
    <name>PDXT_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="1">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxT</name>
        <name type="ordered locus">TV0056</name>
        <name type="ORF">TVG0059350</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916313.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CP5"/>
    <dbReference type="SMR" id="Q97CP5"/>
    <dbReference type="STRING" id="273116.gene:9380821"/>
    <dbReference type="PaxDb" id="273116-14324270"/>
    <dbReference type="GeneID" id="1441543"/>
    <dbReference type="KEGG" id="tvo:TVG0059350"/>
    <dbReference type="eggNOG" id="arCOG00034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069674_2_0_2"/>
    <dbReference type="OrthoDB" id="26717at2157"/>
    <dbReference type="PhylomeDB" id="Q97CP5"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000010">
        <property type="entry name" value="uncharacterized protein LOC100176842 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135695" description="Pyridoxal 5'-phosphate synthase subunit PdxT">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <sequence length="199" mass="22002" checksum="5B71C160A21DB42F" modified="2001-10-01" version="1">MNVGIIGFQGDVEEHIAIVKKISRRRKGINVLRIRRKEDLDRSDSLIIPGGESTTIYKLISEYGIYDEIIRRAKEGMPVMATCAGLILISKDTNDDRVPGMNLLDVTIMRNAYGRQVNSFETDIDIKGIGTFHAVFIRAPRIKEYGNVDVMASLDGYPVMVRSGNILGMTFHPELTGDVSIHEYFLSMGGGGYISTATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CR3</accession>
    <name>Q97CR3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0043618 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0043618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916296.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CR3"/>
    <dbReference type="STRING" id="273116.gene:9380803"/>
    <dbReference type="PaxDb" id="273116-14324252"/>
    <dbReference type="DNASU" id="1441525"/>
    <dbReference type="GeneID" id="1441525"/>
    <dbReference type="KEGG" id="tvo:TVG0043618"/>
    <dbReference type="eggNOG" id="arCOG06046">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507325_0_0_2"/>
    <dbReference type="OrthoDB" id="34235at2157"/>
    <dbReference type="PhylomeDB" id="Q97CR3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="170" mass="20096" checksum="3F4415598E36D47D" modified="2001-10-01" version="1">MCDLAELLMVEHTSIRLLSNFLYGKDSLEIFEGFNDYLVKDHVEIEEKILFPVIVDVDYEDKDQFKATVERIKNDHRLIETLATNLIKWKRDGDDDKFMLRLPLFYKTLTDHNSSEEELIFPRWKNIDPELQKDALKEAISIIDTVNRDLYVRVTGISKDFIYYISGNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CT0</accession>
    <name>Q97CT0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0019869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="1459" mass="158712" checksum="4806F06F2BC02672" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q978E4</accession>
    <name>Q978E4_THEVO</name>
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        <recommendedName>
            <fullName evidence="4">DUF373 family protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">TVG1525115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PaxDb" id="273116-14325712"/>
    <dbReference type="GeneID" id="1442164"/>
    <dbReference type="KEGG" id="tvo:TVG1525115"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048986_1_0_2"/>
    <dbReference type="OrthoDB" id="31282at2157"/>
    <dbReference type="PhylomeDB" id="Q978E4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007254">
        <property type="entry name" value="DUF373"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815:SF1">
        <property type="entry name" value="DUF373 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="329"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="42145" checksum="E722F59171939FF0" modified="2001-10-01" version="1">MTTLIINVDRDNDFGDKAGVNGPVVGYTECYNAALKLITADPEDSDSNALFGALKVYEDLKKKGEDVEIALLTGDNDVGEKSDEILSKQVAEVTAGGEYSDAILVSDGAEDDYIVPVILSYIKIRYVKHIIVRHNENIESIYYYIVKALKDKKIVNKFIIPLGLVFLTYGLVSLVFILYSAYATGVKVIDPTVGAITFVTLVLGVYLLERGFDLIKVVSKFIREVHEYAQDTRILFFTYIIAGLLVLVGIASSYTIAVRTSKNDLDAFLIFLSLFTWWVYGAIFTSEVGRVTELAVGRKVGILKIWYGLIFSLSIAFVVYGMFNYIRYILGFISLKSGLVSIFFLILGLIIAIISSMVHRYFNENPEIQTELPGLEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q978F9</accession>
    <name>Q978F9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1506668</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917687.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978F9"/>
    <dbReference type="STRING" id="273116.gene:9382268"/>
    <dbReference type="PaxDb" id="273116-14325695"/>
    <dbReference type="GeneID" id="1442145"/>
    <dbReference type="KEGG" id="tvo:TVG1506668"/>
    <dbReference type="eggNOG" id="arCOG00198">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_2"/>
    <dbReference type="OrthoDB" id="24644at2157"/>
    <dbReference type="PhylomeDB" id="Q978F9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03214">
        <property type="entry name" value="ABC_Iron-Siderophores_B12_Hemin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051535">
        <property type="entry name" value="Siderophore_ABC-ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42771">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42771:SF2">
        <property type="entry name" value="IRON(3+)-HYDROXAMATE IMPORT ATP-BINDING PROTEIN FHUC"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
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            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="247" mass="27401" checksum="07CB9967B80627B7" modified="2001-10-01" version="1">MIEVTGLEYIRRNFSLNGLNFTITEGSINGIGGLNGSGKTTLVKNLYGFLKPEGGTILIDGKSVESMPIKEIASKVSVVQQEQPIPMNFTVNDVVSLSGYSRKDASISVDECLSLCGILQLKNREFSTLSGGEKRIVMFAAALYQNTKYVLLDEPMTFLDIDKTVTVMSLIRQMKRMGKTIIVVSHDINFLYNFCDYVILMKSGGIAAQGKPQEIFTPDVLKQVFNVEFNRYESAEGTRFYPVIESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>Q978H1</accession>
    <name>Q978H1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1492202 protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="2 3">
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978H1"/>
    <dbReference type="PaxDb" id="273116-14325683"/>
    <dbReference type="KEGG" id="tvo:TVG1492202"/>
    <dbReference type="HOGENOM" id="CLU_2127966_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="113" mass="12795" checksum="28721741AC3B8DCD" modified="2001-10-01" version="1">MESRRIFNVDSDGSEEGKNQDRQNRGHSNNNDRHLGCFDVRSGVFCMAHEGSGGIPMIQDREKKQPENGIKKYTNTDALNTSLEGKIVTISLLNGRFYGYCLFWVVRLLDIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q978H6</accession>
    <name>Q978H6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1488832 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1488832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978H6"/>
    <dbReference type="STRING" id="273116.gene:9382250"/>
    <dbReference type="REBASE" id="685108">
        <property type="entry name" value="TvoVP"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325678"/>
    <dbReference type="KEGG" id="tvo:TVG1488832"/>
    <dbReference type="eggNOG" id="arCOG08993">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1141953_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd22339">
        <property type="entry name" value="NciI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.210.20">
        <property type="entry name" value="MvaI/BcnI restriction endonuclease, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3570">
        <property type="entry name" value="MvaI/BcnI restriction endonuclease, recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029127">
        <property type="entry name" value="MvaI_BcnI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043004">
        <property type="entry name" value="MvaI_BcnI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043005">
        <property type="entry name" value="MvaI_BcnI_rec"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15515">
        <property type="entry name" value="MvaI_BcnI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MvaI/BcnI restriction endonuclease" evidence="1">
        <location>
            <begin position="32"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="283" mass="33059" checksum="2623F052436D30EF" modified="2001-10-01" version="1">METINTERNLRQTHLIGMVQMSQPVYSKQELIERLKEIRDKGWIQNRRKGNTGGIGNTLEDLLEIKENNLPIPNSAEWELKTHRKNNGSLTTLFHSEPSPRSLKIVPSILLPYYGWKHEKAGTKYPDSEMSFRQTINAVTRTDRGFCIVIDYDEEKILISFDPGKVSPKHKEWLTSVEERVGHIGELDIQPYWGFDDLFHTAGSKLLNCFYIDAEVKKEGDQEYYLYKDIYMLRKFSKQKFIKAIQNGIVLIDFDARTGHNHGTKFRIREDKIPILYEEVSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q978K8</accession>
    <name>Q978K8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1453486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917639.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978K8"/>
    <dbReference type="STRING" id="273116.gene:9382216"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325646"/>
    <dbReference type="GeneID" id="1442097"/>
    <dbReference type="KEGG" id="tvo:TVG1453486"/>
    <dbReference type="eggNOG" id="arCOG03664">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_595322_0_0_2"/>
    <dbReference type="OrthoDB" id="55818at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019187">
        <property type="term" value="F:beta-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027389">
        <property type="entry name" value="B_mannosylTrfase_Bre-3/Egh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16779">
        <property type="entry name" value="BETA-1,4-MANNOSYLTRANSFERASE EGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16779:SF1">
        <property type="entry name" value="BETA-1,4-MANNOSYLTRANSFERASE EGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="383"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="181"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="459" mass="52582" checksum="430296DD22D8916B" modified="2001-10-01" version="1">MQTVRTPVLNLGLTFWQIPIILVFLIPVGFTVVSGGSSPITYAIEALWFMGLPVIIIALAGAYKSRDVRLSMFSGKINDTVIFQVPSIARRDLLNALERVISSVIMNAPKNLENWLLNIVIEESSEGKYYLKNKYGKNPRINIIEVPASYKTKNDSLAKSRANQYAIEYLRERGLVNNRTWIYHLDDDTSIGPDTVAAIAEHIFLHGSEYYLAQGILSFPHYLTKSIITRFADSMRPTDDLTRFYFFTNVVKMPLIGLHGENMLVRSDIEESVGWDNGNRAIADDSYFALRFAYMYPGRSSFMPAVTYGSSPSTIRDMLRQRKRWFLNVSNLSFFGNVPLRYRAFMIYSIIFWVGMVIQNVVPILLLLHILGVMKMPIINDEVLGLWAFTLSFWIWFYLSGLKINKEICGDYKHRFMDQLVLVFLYIFVISPLEIVGGLLGFISFLRNEKRFDVVSKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q978L8</accession>
    <name>Q978L8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1442264 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1442264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917629.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978L8"/>
    <dbReference type="PaxDb" id="273116-14325636"/>
    <dbReference type="GeneID" id="1442087"/>
    <dbReference type="KEGG" id="tvo:TVG1442264"/>
    <dbReference type="eggNOG" id="arCOG05345">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170818_0_0_2"/>
    <dbReference type="OrthoDB" id="56446at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="113" mass="13369" checksum="103944CA8D908F75" modified="2001-10-01" version="1">MDDDLEKYFKEFMKIMPTEDLYFEAVRDIVKDLIKEYIKKKMNEDETIRKDLARVIEEFMDARIKEYDAVASMAKIVAKIGLVSAPDDVKEQVYNDFITMFQKEIEGIIKKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q978M0</accession>
    <name>Q978M0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Uroporphyrin-III C-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1441436</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052552">
        <property type="entry name" value="YeaO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36849:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36849">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22752">
        <property type="entry name" value="DUF488-N3i"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14812" checksum="01D967C7EEEF5999" modified="2001-10-01" version="1">MLSIKRVYENRTYDDGYRVLVERLWPRGITKERAALDAWYKDIAPSDSLRKWFSHDERKWDEFKERYMDELCGNKALEKLVEIASQTNVTLIISSRSTYNNAEVLKYIIENYDEFKRRCEGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q978M7</accession>
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            <fullName evidence="5">TVG1434253 protein</fullName>
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        <name evidence="5" type="ORF">TVG1434253</name>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
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        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="137" mass="14711" checksum="79F39644EC89ABA7" modified="2001-10-01" version="1">MGALIMIFTADHFERKYVIFADTVVWFIGLFLFSIKIGTTIFIGSFLASMALGMYLQVAYTYTAENYPTRARSSGFALTDGIGHVGGALGALLLPVIVSAYSFSFGFKFIAITGLIAGILALFGPKSSKLTLEEISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q978N6</accession>
    <name>Q978N6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="7 8">
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="273116.gene:9382187"/>
    <dbReference type="PaxDb" id="273116-14325618"/>
    <dbReference type="KEGG" id="tvo:TVG1425726"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_20_3_2"/>
    <dbReference type="PhylomeDB" id="Q978N6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="4"/>
            <end position="296"/>
        </location>
    </feature>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="317" mass="34126" checksum="912E39D0828FFCC1" modified="2001-10-01" version="1">MNYSMISASIEIGFLILMGIIIAIKVGSGNTISVFTLKPLNGNFSLLFFAMIFSIPLFAGSGTVITLAEETKGSIKTIRRSIWISMLVLLAALLVPAYSLTVGYGVSNMAGFTQLPDPGVIIFERYGGIVAGIILIILTVNSYLSSNVSLMSSVSRVVYSLGRDGILPKSITKIHPIHHSPYISVMLLEIGGIVIAIIPSIFYGPFTGALILFSINIFALLFTHMLVNASLPVYIKRNGLHFNAITHLVLPILSIIIGLIVVYYSARTSISEGNPITAAVFIGLAYVAFAVIFGYSFAKYHPDKVKNIGSISIEEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Q5</accession>
    <name>Q978Q5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fumarase</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">TVG1409429</name>
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    <organism evidence="4 5">
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        <lineage>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="BAB60502.1"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
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        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
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    </dbReference>
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        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
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        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
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        <property type="entry name" value="PRK12273.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASPARTATE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42696:SF2">
        <property type="entry name" value="ASPARTATE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
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        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
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    </dbReference>
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        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarase C C-terminal" evidence="3">
        <location>
            <begin position="407"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="457" mass="50265" checksum="DCBA0C6DEB35E190" modified="2001-10-01" version="1">MRTERDVIGEIQLEDTVFYGINTARAKENFRITGLTSDSDHIVSIAQIKKAAAMANAEAGALDKEKAEFIEKACDEIISGRYHDQFVIDVYQAGAGTSFNMNANEVIANVALTLMGKNKGDYSVIHPNDHVNMSQSTNDVYPTMMRLTVTKKVKKLKEAISDLIESLEKKSKELRGIPKPGRTHLQDAAPVTVGLEFSAYAYAIKKDRDEITDALDYIMELNIGGTAVGTGINTSKNYQENVVKHIAEITRENFRKSSNLMGIMQFMTDFSRVMNAVTNLALDIEKIANDIRLLYSGPGTGIHEIIIPAVQQGSSIMPGKINPSIAEAMNMICHSVIGAQQAVNMSVQAGQLELNVMMPNIDYELTRSIDIMRNGLIMFKEKLIDGIKANVNTCREHLANSFGSAALLNPYLGYDNVAKIVREAVETGKSIKSLVLATGKITEEQYMKIMESGVPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Q9</accession>
    <name>Q978Q9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Solute-binding protein family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1401500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Q9"/>
    <dbReference type="STRING" id="273116.gene:9382164"/>
    <dbReference type="PaxDb" id="273116-14325595"/>
    <dbReference type="KEGG" id="tvo:TVG1401500"/>
    <dbReference type="eggNOG" id="arCOG01533">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_319962_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978Q9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.76.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF34">
        <property type="entry name" value="ABC TRANSPORTER, PERIPLASMIC OLIGO-PEPTIDE BINDING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="2">
        <location>
            <begin position="383"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="963" mass="106976" checksum="793D754012633CB3" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q978S9</accession>
    <name>Q978S9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hypohtetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1380120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917571.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978S9"/>
    <dbReference type="STRING" id="273116.gene:9382144"/>
    <dbReference type="PaxDb" id="273116-14325575"/>
    <dbReference type="GeneID" id="1441453"/>
    <dbReference type="KEGG" id="tvo:TVG1380120"/>
    <dbReference type="eggNOG" id="arCOG01357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032214_0_0_2"/>
    <dbReference type="OrthoDB" id="358785at2157"/>
    <dbReference type="PhylomeDB" id="Q978S9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF4">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="246"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="558" mass="62688" checksum="23ED78F1A215E827" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q978U7</accession>
    <name>Q978U7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1359071 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1359071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917553.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978U7"/>
    <dbReference type="STRING" id="273116.gene:9382126"/>
    <dbReference type="PaxDb" id="273116-14325557"/>
    <dbReference type="GeneID" id="1441435"/>
    <dbReference type="KEGG" id="tvo:TVG1359071"/>
    <dbReference type="eggNOG" id="arCOG06958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181238_0_0_2"/>
    <dbReference type="OrthoDB" id="57159at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="99" mass="11659" checksum="3D6044E445730AB0" modified="2001-10-01" version="1">MKRQQFEKRGFSDRGEFQKREKKLDMSRSEEFGYMLGKTVEGLPDSVRGAIRGTIYSLASKKGIKEAKEFIGRKYQEGVIDYDTQKKLINLIYDYSKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q978V2</accession>
    <name>Q978V2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1353500 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1353500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917548.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978V2"/>
    <dbReference type="STRING" id="273116.gene:9382121"/>
    <dbReference type="PaxDb" id="273116-14325552"/>
    <dbReference type="GeneID" id="1441430"/>
    <dbReference type="KEGG" id="tvo:TVG1353500"/>
    <dbReference type="eggNOG" id="arCOG03704">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045931_0_0_2"/>
    <dbReference type="OrthoDB" id="56193at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="472" mass="52180" checksum="951318097D49B169" modified="2001-10-01" version="1">MDTLKVALGVAIALVIVFAGAFGYSYYQNSKTSSAYSTAQANAQDYKPNAVLGDAFEHWNNIAIENTTLVSSQYLSNATLQWIGGPLSGTYTGLSAIDSTWNKFFGLWSAVWLYTSNPPVVTTSGDTAQVTAPVQFIVTPFNVNNTVEYINVSYTLDFMKSGGQFYITHEVWHITGAGILASVPSAVLTQEVEAQAMEHWDYIAIENTSLLQPEYASNATLDWIGGPLTGTYSGVSSIIGTWGKFFGLWSAVWFYTVTPPSVSVIGKTATVTSMNQFIVTPTSANNTVEYLNISYTLNYYKEMGQYKIVGETWHIVGTGYISTNENQDMYNQVLALAFSHWNNIAIENLTSVMEQYNDSATLYWVNSTLNGTYQGYSNISAVWNKFFKAWIAVWFYAEEPPTVAMKGDMAYVNATVQFVVQKNATTFDYINVSYQIVYQNMGFNVKTGQFQFEIVSEVFNVLGGAPGPLNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q978V4</accession>
    <name>Q978V4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">dCMP deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1352610</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917546.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978V4"/>
    <dbReference type="STRING" id="273116.gene:9382119"/>
    <dbReference type="PaxDb" id="273116-14325550"/>
    <dbReference type="GeneID" id="1441428"/>
    <dbReference type="KEGG" id="tvo:TVG1352610"/>
    <dbReference type="eggNOG" id="arCOG01487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047993_2_3_2"/>
    <dbReference type="OrthoDB" id="7284at2157"/>
    <dbReference type="PhylomeDB" id="Q978V4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016473">
        <property type="entry name" value="dCMP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086:SF18">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006019">
        <property type="entry name" value="dCMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="6">
        <location>
            <begin position="8"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="170" mass="19095" checksum="B57C46B56CA26088" modified="2001-10-01" version="1">MELVKRPSWDEYFMRMAYLAASRTNCIRRKVGAVIVKDKNVLATGYNGPPSGTAHCDVVGCIREDLKIPSGERHELCRGLHAEQNAIIQAAVHGVSIKDSTIYVTTHPCVVCSKMIMNAQIKEIVYAEGYPDELAELMLLESNVKVRRFSLPEKEVRAMIGDFYYLEGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q978V9</accession>
    <name>Q978V9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1347387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917541.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978V9"/>
    <dbReference type="STRING" id="273116.gene:9382113"/>
    <dbReference type="PaxDb" id="273116-14325545"/>
    <dbReference type="GeneID" id="1441423"/>
    <dbReference type="KEGG" id="tvo:TVG1347387"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_2"/>
    <dbReference type="OrthoDB" id="6342at2157"/>
    <dbReference type="PhylomeDB" id="Q978V9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047110">
        <property type="entry name" value="GABD/Sad-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43217">
        <property type="entry name" value="SUCCINATE SEMIALDEHYDE DEHYDROGENASE [NAD(P)+] SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43217:SF1">
        <property type="entry name" value="SUCCINATE SEMIALDEHYDE DEHYDROGENASE [NAD(P)+] SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="436" mass="48547" checksum="A30AFB9F51F7C318" modified="2001-10-01" version="1">MIKTLNPYTGEVIASYNEEDKEQVIRKIKDLREYQAKWRKNIGERIEVIKAAKKNFETQIDDLSKLMSLEMGKPISQSISEVKKSIWLMDYVIENAEKFLAIEDVKTEARKSYVRFDPIGVILIIMPWNFPVWQVMRAAIPALAAGNTVILKHASIVSGTSLKIQDLLNTDAFRSVITSGETASSVIPEVDGVSFTGSTSTGSRIAEIAGKNIKKFVMELGGSDPFIVLDDYNLEDTVKNAVFGRLQNNGQSCIASKRFIVNRKIGEKFVEMLKDEFSRVKIGDPLNSDTFLGPLSSQDQKETVLRQVSDLKGKSELVIGGDYQGNIVMPTIIKTDVRYNEEIFGPVAVVKYFDKPEEAVEISNETPFGLGASIWGNPDEAEKLIPEIQAGMVFVNKIVASDPRLPFGGVKKSGVGRELSRYGMLEFTNIKTVWIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q978X8</accession>
    <name>Q978X8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1326678 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1326678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978X8"/>
    <dbReference type="STRING" id="273116.gene:9382093"/>
    <dbReference type="PaxDb" id="273116-14325525"/>
    <dbReference type="KEGG" id="tvo:TVG1326678"/>
    <dbReference type="eggNOG" id="arCOG03660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726883_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978X8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052714">
        <property type="entry name" value="MFS_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531:SF1">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="367" mass="40690" checksum="8BE3375A44DE7F12" modified="2001-10-01" version="1">MFMTEIPLVARYIIHFNEFLVGSVSAVGALATFVMSAFINSKLSSTLRRKVFIASSLAYALVFPTFYFSNSITIWPIVFVSGFVLGAIMPNIITSASLLPDRKARERVLSIYTLTLSISLVAGPAIEGYLLRIYPLLETFIFFSIFPALVFVTSFFIKFPEEQSNVKIEAKNVIKNHGFKVSVYNIMTYNIPFAFILTFGGIYARDFFGASYSTVTLMFSSFFFTSFISRLLLALKPPENLWRLMILSVLLTTVGLMGIVESPSLTVMELSMLILGFPHGFTYPLSVISISRAFDISSRNAANSLFFSIMMAIGSVMPFVSGSVVLYIGLKYSFVSVIPVILIILLLLHHEMKLLSIGKSGGQYTAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-16" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Y8</accession>
    <name>SYW_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="1">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpS</name>
        <name type="ordered locus">TV1276</name>
        <name type="ORF">TVG1316213</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917511.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Y8"/>
    <dbReference type="SMR" id="Q978Y8"/>
    <dbReference type="STRING" id="273116.gene:9382082"/>
    <dbReference type="PaxDb" id="273116-14325515"/>
    <dbReference type="GeneID" id="1441393"/>
    <dbReference type="KEGG" id="tvo:TVG1316213"/>
    <dbReference type="eggNOG" id="arCOG01887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032621_3_0_2"/>
    <dbReference type="OrthoDB" id="371821at2157"/>
    <dbReference type="PhylomeDB" id="Q978Y8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_A">
        <property type="entry name" value="Trp_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020653">
        <property type="entry name" value="Tryptophan-tRNA-ligase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008926">
        <property type="entry name" value="PRK12285.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF5">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136736" description="Tryptophan--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="66"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="314"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <sequence length="426" mass="48768" checksum="7884711A187319E2" modified="2001-10-01" version="1">MINPWSSSDFFDYERLKKEFGISDQSDNIDHFLFRRKVILGQRGFEYIKYAIDNKIKFNVMTGLMPSGEMHLGNKSAIDQVIYFQKLGGSVSIAVADLESYSTRGIPLDKAREIAIEKYILNYIAMGLQPCEIYFQSKNKDVQFLSYILGNWTNMNELKALYGFTDSNDILHINAPLIQAADVLHTQLNNYGGPAPTVVPVGFDQDPHIRLMRDLAKRMRIFNVFYDGGITVSIKGKGDSTMPVDQAYEYLSKRFSEVTKDYEYRVVKAKDGKEEDIVRTDIDLAKIGSEFNVFSFIPPSATYQKLMKGLKGGKMSSSVPDSLISMNDDVEEAKRKIMRALTGGRDTEEEQRKLGGEPEKCPVFDLYNYEIDDDKYVNEVFEECKSGKRMCGYCKREIADKMSIFLKDIKEKREIAREKLSLYIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q979A6</accession>
    <name>Q979A6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">2-hydroxyhepta-2,4-diend-1,7-dioate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1294303</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917490.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979A6"/>
    <dbReference type="SMR" id="Q979A6"/>
    <dbReference type="STRING" id="273116.gene:9382061"/>
    <dbReference type="PaxDb" id="273116-14325494"/>
    <dbReference type="GeneID" id="1441372"/>
    <dbReference type="KEGG" id="tvo:TVG1294303"/>
    <dbReference type="eggNOG" id="arCOG00235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_3_0_2"/>
    <dbReference type="OrthoDB" id="6242at2157"/>
    <dbReference type="PhylomeDB" id="Q979A6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000002">
        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051121">
        <property type="entry name" value="FAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796:SF4">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="62"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="268" mass="29642" checksum="BDD18873F7BC081F" modified="2001-10-01" version="1">MKIGRCLYQGKETLFLFSNGKYFSINGKSDINEIIKTGADKIDVGNEISNFQFLPAVNPQKIFLPAVNFRSHSAESDTRFPPKPYFFTKFSNALVANNSELRTPGTIKKLDYEGETGAVIGKKCYNIPAEDAYDCIFGFTIANDLSARDFQFPEMHPYGYNWVQGKAFDGALPVGPYITTKDEIQFPLEIETKVNGETRQKGTTDDMVFSFQDLISTLSQNITLEPGDIITSGTPAGVAAFSEKLYLKSGDVIEISVSSLGTLMNKIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q979B8</accession>
    <name>SYK_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="1">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lysS</name>
        <name type="ordered locus">TV1243</name>
        <name type="ORF">TVG1282914</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917477.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979B8"/>
    <dbReference type="SMR" id="Q979B8"/>
    <dbReference type="STRING" id="273116.gene:9382048"/>
    <dbReference type="PaxDb" id="273116-14325481"/>
    <dbReference type="GeneID" id="1441359"/>
    <dbReference type="KEGG" id="tvo:TVG1282914"/>
    <dbReference type="eggNOG" id="arCOG00485">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025562_0_0_2"/>
    <dbReference type="OrthoDB" id="6838at2157"/>
    <dbReference type="PhylomeDB" id="Q979B8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.10">
        <property type="entry name" value="Lysine tRNA ligase, stem contact fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042078">
        <property type="entry name" value="Lys-tRNA-ligase_SC_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940:SF1">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152763" description="Lysine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="23"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="267"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00177"/>
        </source>
    </evidence>
    <sequence length="503" mass="56997" checksum="EF15FED06BC11B56" modified="2001-10-01" version="1">MFWADAAVKDLIGEQRISTGISPSGPIHVGNMREILTGDILYKAVIKRGLKSDFIYLCDDMDPLRKVYPFLDQSYSKYVGFPLKNIPSPDGVGVYSDHFLNPFIEVMRKTGIPARVIKTSDLYGNGILADAIDTVMERRSEIKDILEKITGRMIEGDFYPYEPLCEKCGRINSTSVISYKYPYAEYTCKCGHHGFADIRRAEGKMPWRIEWPAKWYALKVSIEPFGKDHGAPGGSYDTGKRIAREIFGIEPPLPLVYERIMLKGKGAMHSSTGLAIPASEIIDVMPPELLRFLIARVNPSRHIDFDPGLGLLNLFDEFERYLNKYREGDIDGDSKAIIEYSLIEKEKLSYPIDFRHLITLIQIYQKKEDILRAAMASTKDQLDESALYDEIKYAKNWLERYAPDNVKFKLLAIDEKAELSDQDRAILSKFLEVSNSMAWDSNEIHSTVHNVAKDLGIGPDAAFSTFYKVFIGKERGPRLGYFLFNLGKDFTIARIKSVLGGNS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q979F7</accession>
    <name>IF1A_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 1A</fullName>
            <shortName evidence="1">aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">eIF1A</name>
        <name type="ordered locus">TV1204</name>
        <name type="ORF">TVG1232812</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
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        <text evidence="1">Belongs to the eIF-1A family.</text>
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    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
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        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
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        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="S1_IF1_TYPE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <feature type="chain" id="PRO_0000145137" description="Translation initiation factor 1A">
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            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="S1-like" evidence="1">
        <location>
            <begin position="28"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="123" mass="14531" checksum="D08E08B0B71C53F6" modified="2001-10-01" version="1">MSPDKTEDEDKDVNVDQDQFNEEEESLGRVILPNKKKGEMFGIVEKMEGASRLSVMCEDGYTRNARIPGRMRKRMWIREKDLVIVKPWEFQPEKADVVYRYTKTQASYLSRNHMLPEVIDIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q979J8</accession>
    <name>Q979J8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1190485 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1190485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979J8"/>
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    <dbReference type="GeneID" id="1441278"/>
    <dbReference type="KEGG" id="tvo:TVG1190485"/>
    <dbReference type="eggNOG" id="arCOG07388">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_588789_0_0_2"/>
    <dbReference type="OrthoDB" id="56788at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
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        <location>
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            <end position="97"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="464" mass="50957" checksum="F1781028567D0B72" modified="2001-10-01" version="1">MNTNLIFDQKKNITALTLFIAVFLIISSLFGVTWFFLGDFTYNAVNYYHVIMIPFALLLIIISAFIVKLPNIFQKAINSVTYPVIIFTVLGLAFFYYPSLTIADEYMQAIRDFIMLIIGLVFIVGLIYYPFKDKYNFKTIWGAYSFLAIAAIAAEIAAVYGLITAAGTFIGFSKIPILQSYINSLNETDSTFMGNIVTSHSHAMLPAVMGGIVALTALAFNYEKLPSRCRSIVNWAFVISIFGTLSMSYLYWISGLGTYVIPTIAPFGPGGMDGLALDDSQTGIVGWGALIAIAGLWYTATRNGKLDKLRIAEFATWIFTMIAMIGIGYVIEFNEAFYGFATPGVPPNGGPGYLYDMAFINGHLLYAFFLMPVLALVIVAADLMIPEENAGTRNLIGYLAISGMVIGVAGLQLYTMTLHWQVEALGLYLIVISILLMGVFLAMSFRGIVMSKRSESKSKVSTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q979L1</accession>
    <name>Q979L1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1179765 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1179765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
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            <taxon>Thermoplasmatota</taxon>
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            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979L1"/>
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    <dbReference type="PaxDb" id="273116-14325388"/>
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    <dbReference type="KEGG" id="tvo:TVG1179765"/>
    <dbReference type="eggNOG" id="arCOG07399">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1821119_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="141" mass="16459" checksum="B39F3E558DD62EFB" modified="2001-10-01" version="1">MKMKAHTDCKKIRESVLLVKTQNGSSYRIFGELADRIIKGSEKMHMEITDYIKRYNKFKDIIEAHIICRSNKNMDIQSKYLTITISFKEYMVLGAKNTLTIRGKEAMKIKEQADKLKMGIDEYLKSPLSPYKWVESITVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q979M5</accession>
    <name>Q979M5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH dehydrogenase I chain F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1162786</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
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    <dbReference type="STRING" id="273116.gene:9381934"/>
    <dbReference type="PaxDb" id="273116-14325373"/>
    <dbReference type="GeneID" id="1441251"/>
    <dbReference type="KEGG" id="tvo:TVG1162786"/>
    <dbReference type="eggNOG" id="arCOG01543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067218_4_5_2"/>
    <dbReference type="PhylomeDB" id="Q979M5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004545">
        <property type="entry name" value="PRK05888.3-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="88"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="143" mass="16875" checksum="27E24430C2D3CED5" modified="2001-10-01" version="1">MIEVKEPKRIPFVGMLQGMFTVFKHLFKKPITVQYPEEKAYTPPRFKFRIFLSMDDCVGCTLCEQVCPNLSIRMIVVERNNPHNKRNLYPQVNFGTCTVCRNCEEICPTEAIYLTHELETARTRNNFFYSPQELEKTEEEVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q979N3</accession>
    <name>Q979N3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1156690 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1156690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_156769112.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979N3"/>
    <dbReference type="STRING" id="273116.gene:9381926"/>
    <dbReference type="PaxDb" id="273116-14325365"/>
    <dbReference type="GeneID" id="1441243"/>
    <dbReference type="KEGG" id="tvo:TVG1156690"/>
    <dbReference type="eggNOG" id="arCOG05378">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140105_0_0_2"/>
    <dbReference type="OrthoDB" id="56064at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="116" mass="13805" checksum="895956AD82F96801" modified="2001-10-01" version="1">MNGDLNMDVVRSALLAGEIDDAYKVVFNAVRRLDLYRRSTGDRRYDVYFNFLTLLQDVLEGKKGWKSISDYIRDNPDISKYVDSNFLSSFPYYLFYSVDRYNVRFPYFDGKRCDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q979P4</accession>
    <name>Q979P4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1148602 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1148602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917350.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979P4"/>
    <dbReference type="PaxDb" id="273116-14325354"/>
    <dbReference type="GeneID" id="1441232"/>
    <dbReference type="KEGG" id="tvo:TVG1148602"/>
    <dbReference type="eggNOG" id="arCOG05393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392760_0_0_2"/>
    <dbReference type="OrthoDB" id="56414at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="95" mass="11047" checksum="64CB42C0AFB73011" modified="2001-10-01" version="1">MKIEIGQRIDVEVDREDVERVSRGSIIAVWYNRGVPIYVELFVNKTLISEIRKMFNNNNRKSALVSITRISKSKYVVEPTVVVLNRQRTDLTPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q979R9</accession>
    <name>Q979R9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1121766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917323.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979R9"/>
    <dbReference type="STRING" id="273116.gene:9381888"/>
    <dbReference type="PaxDb" id="273116-14325329"/>
    <dbReference type="GeneID" id="1441205"/>
    <dbReference type="KEGG" id="tvo:TVG1121766"/>
    <dbReference type="eggNOG" id="arCOG00434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034716_2_0_2"/>
    <dbReference type="OrthoDB" id="24581at2157"/>
    <dbReference type="PhylomeDB" id="Q979R9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050764">
        <property type="entry name" value="CbbQ/NirQ/NorQ/GpvN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42759:SF1">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42759">
        <property type="entry name" value="MOXR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002849">
        <property type="entry name" value="AAA_ATPase_chaperone_MoxR_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="41"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="319" mass="36376" checksum="0A66FB41E2554D1E" modified="2001-10-01" version="1">MESTSELEDLRNTVVSIEESIGKRVIGSRRIVRFMFIALLTGNHILLEGVPGLAKTMLANEFARHTKMEFKRIQFTPDMLPSDITGTIMFNIESRKMEFKEGPLFANVVLADEINRTPPKVQSALLEAMEEVHVTVGGETHSLPKPFFVIATQNPVEQEGTFPLAEALMDRFLFRYYLDYPSREDEINILNSMGNSEDPSIVLLPDEIMHFRSEVDNVYVSDEIKEYIVDLMRKTREDELVYLGASPRTTSKYIKAARANALISGRSYVIPEDILYMAFEILNHRLILKPEAVVDETDDIKNIVKRIVDKVVSETPYPK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q979T1</accession>
    <name>EF1A_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor 1-alpha</fullName>
            <shortName evidence="2">EF-1-alpha</shortName>
            <ecNumber evidence="2">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Elongation factor Tu</fullName>
            <shortName evidence="2">EF-Tu</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tuf</name>
        <name type="ordered locus">TV1079</name>
        <name type="ORF">TVG1107082</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60221" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60221.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917309.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979T1"/>
    <dbReference type="SMR" id="Q979T1"/>
    <dbReference type="STRING" id="273116.gene:9381875"/>
    <dbReference type="PaxDb" id="273116-14325317"/>
    <dbReference type="GeneID" id="1441191"/>
    <dbReference type="KEGG" id="tvo:TVG1107082"/>
    <dbReference type="eggNOG" id="arCOG01561">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_3_5_2"/>
    <dbReference type="OrthoDB" id="371718at2157"/>
    <dbReference type="PhylomeDB" id="Q979T1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000090998" description="Elongation factor 1-alpha">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="5"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="70"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="187"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="91"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="424" mass="46935" checksum="008B8A7DE45D64A7" modified="2002-05-27" version="2">MASQKPHLNLITIGHVDHGKSTLVGRLLFEHGEIPAHIIEEYRKEAEQKGKATFEFAWVMDRFKEERERGVTIDLAHRKFETDKYYFTLIDAPGHRDFVKNMITGTSQADAAILVISAREGEGVMEQTREHAFLARTLGVPQIVVAINKMDATEPPFSEKRFNEVKADAEKLLKTIGYKDATFVPISGYKGDNVTKPSPNMPWYKGPSLLQALDAFKVPEKPINKPLRVPVEDVYSITGIGTVPVGRVETGVLKPGDKVIFLPADKQGDVKSIEMHHEPLQQAEPGDNIGFNVRGIAKNDIKRGDVCGHLDSPPTVVRAFTAQIVVLNHPSVIAPGYKPVFHVHTAQVACKIDEIVRTLNPKDGTTLKDKPDFIKTGDIAIVKVIPDKPLVIEKVSEIPQLGRFAVRDMGQTVAAGQCIDLEKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q979U4</accession>
    <name>SYFA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheS</name>
        <name type="ordered locus">TV1066</name>
        <name type="ORF">TVG1092683</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917295.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979U4"/>
    <dbReference type="SMR" id="Q979U4"/>
    <dbReference type="STRING" id="273116.gene:9381861"/>
    <dbReference type="PaxDb" id="273116-14325304"/>
    <dbReference type="GeneID" id="1441177"/>
    <dbReference type="KEGG" id="tvo:TVG1092683"/>
    <dbReference type="eggNOG" id="arCOG00410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025086_2_2_2"/>
    <dbReference type="OrthoDB" id="372178at2157"/>
    <dbReference type="PhylomeDB" id="Q979U4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00282">
        <property type="entry name" value="Phe_tRNA_synth_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022917">
        <property type="entry name" value="Phe_tRNA_ligase_alpha_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000126823" description="Phenylalanine--tRNA ligase alpha subunit">
        <location>
            <begin position="1"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="366"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with beta subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00282"/>
        </source>
    </evidence>
    <sequence length="482" mass="55848" checksum="2978BABC7883DF2F" modified="2001-10-01" version="1">MSEISKNEALILKYLASKNQEIEESNIEIKGLSRQDIASATSWLQVKGLIDVKTREEVAYVLTDEGKRYAENGLPELRAYSILKRKGKLSLRDLQEAMPDEYKIVLAQLAKFGITPKNGVLEYSDGHIEAEISRRQRFLSDLNTDDQEMIEHFKRRKNVIEEKKRSVRIVSINSKGLEQLNNFDQFEAIGEIDSGIITSQAWKTAPFRKYDLDAPVSPTKSYAKHPLVYFINEIRRIFLDMGFTEMSGHYIESALWDMDALFIPQDHPARDMQDTFYVKDSNFTIEHPEIEKRIKRIHERGFDGYSGWGYRWSSDDGKKLILRTHTTVTTARYLYEHNTYPQAIFSVEKVFRHESVDWKHLAEFYQIEGAVYDKNVSVATLKWILRTFYGKLGFEKIRLVPSYYPYTEPSLDVVVEVDGKEMELGGSGIFRPEVGKILGLKAPVMAWGMGLERLAMLYYGLTDVRDLYNTDFSFLEGYKIKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q979U7</accession>
    <name>NADK_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD kinase</fullName>
            <ecNumber evidence="1">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadK</name>
        <name type="ordered locus">TV1063</name>
        <name type="ORF">TVG1088770</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917292.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979U7"/>
    <dbReference type="SMR" id="Q979U7"/>
    <dbReference type="STRING" id="273116.gene:9381857"/>
    <dbReference type="PaxDb" id="273116-14325301"/>
    <dbReference type="GeneID" id="1441174"/>
    <dbReference type="KEGG" id="tvo:TVG1088770"/>
    <dbReference type="eggNOG" id="arCOG01348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_0_0_2"/>
    <dbReference type="OrthoDB" id="77798at2157"/>
    <dbReference type="PhylomeDB" id="Q979U7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002255">
        <property type="entry name" value="PRK01185.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000120712" description="NAD kinase">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="62"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="129"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="170"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <sequence length="272" mass="29876" checksum="05AD7B7DA45E6381" modified="2001-10-01" version="1">MKVAFVIRKDCKRCATIAKSIIELIPPDWEKIYDTEAAKFLGGVGKDITEISADIIIAIGGDGTVLRILQNAKGPVLGINMGGLGFLTEIEIDEVGSSTYKLIRGEYKINEAMKLKVYINGRRLEDCTNEAVVHTDRIARIRQFKIYVDGHFLTTIKSDGVIVATPTGSSSYSSSAGGPLLLPTVRGMVISYLAPYSSRIKPVVVPSESTVEIKIAGNDQDSLLILDGQKEYKIKSGDTVSISMSEEKARFVSFRESIYDRLRDKVIKHVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Y5</accession>
    <name>Q979Y5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1047701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917253.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Y5"/>
    <dbReference type="STRING" id="273116.gene:9381818"/>
    <dbReference type="PaxDb" id="273116-14325263"/>
    <dbReference type="GeneID" id="1441135"/>
    <dbReference type="KEGG" id="tvo:TVG1047701"/>
    <dbReference type="eggNOG" id="arCOG07382">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965119_0_0_2"/>
    <dbReference type="OrthoDB" id="56185at2157"/>
    <dbReference type="PhylomeDB" id="Q979Y5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="33953" checksum="C760F2B3778DAD1D" modified="2001-10-01" version="1">MVESIPTKDVRFKIQEDLDRRVKESGKTYKEIIYEYFNGSQNNDNIPEWVFEPEKANRIILFNHFKSLELNDYNKKFVRYVYHRITQMLEKNPSDEKLKDLREFVIEDMTNIESPFELDLVREAMKPIMEVKDSIYSDNGLVGEKKAIESEIGYLNSKKDQMQSEISQLSAEINDLNAKKSELEQAIAEMQKQIEDIQAGIDERIKAVFEENESMKKTLEEYEKTRADLATALSTVSQLKDLILQRDAEIKKLKDSYNDIFTRYDTLINKMTKREFEKINARELKKSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Y8</accession>
    <name>SRP54_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Signal recognition particle 54 kDa protein</fullName>
            <shortName evidence="1">SRP54</shortName>
            <ecNumber evidence="1">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">srp54</name>
        <name type="ordered locus">TV1022</name>
        <name type="ORF">TVG1046103</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917250.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Y8"/>
    <dbReference type="SMR" id="Q979Y8"/>
    <dbReference type="STRING" id="273116.gene:9381815"/>
    <dbReference type="PaxDb" id="273116-14325260"/>
    <dbReference type="GeneID" id="1441132"/>
    <dbReference type="KEGG" id="tvo:TVG1046103"/>
    <dbReference type="eggNOG" id="arCOG01228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_2"/>
    <dbReference type="OrthoDB" id="52849at2157"/>
    <dbReference type="PhylomeDB" id="Q979Y8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0733">Signal recognition particle</keyword>
    <feature type="chain" id="PRO_0000101190" description="Signal recognition particle 54 kDa protein">
        <location>
            <begin position="1"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="104"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="184"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="242"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <sequence length="455" mass="51053" checksum="034FF5C64307A950" modified="2001-10-01" version="1">MVLESLSSSLRETIRKITGSTYIDKNTVKEISKDLQRILLKADVNVQTVLNITKEMERRALEEKPPVGMAHQDYMVKIIYEELLKILGEPSNVELKPQTIMLVGLYGHGKTTSAGKLSRFFMKKGLSAGLIAADVHRYAAYEQLQQIAESVNAKFYGDPKEKDPVKIIKNGLEVLKDAQVKIIDTSGRDSLDSDLIEEIRRIKEAVKPDQVLMVIDATMGQQAGPESKAINDAVGVTGIIITKMDGTAKGGGALSAVAKMRVPIYFIGTGEHMDDMEIFDPKKFLSRLLGLGDLETLFETVKEANITEEEAQKSFEKLMTGKFNLKDMYDVWEKFSRPGLMKKLVDSLPLAKLPGSQKINDDQILTAEQKLQRYRIIMDSMTFQELENPEIINAKRITRIARGAGVREEDVRMLLKEFKAMKNNMKLMKGNRGFKKILQSNFRSGNFGLDDLGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A04</accession>
    <name>Q97A04_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyruvate, phosphate dikinase</fullName>
            <ecNumber evidence="3">2.7.9.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">TVG1029756</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.1" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917234.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A04"/>
    <dbReference type="STRING" id="273116.gene:9381799"/>
    <dbReference type="PaxDb" id="273116-14325244"/>
    <dbReference type="GeneID" id="1441116"/>
    <dbReference type="KEGG" id="tvo:TVG1029756"/>
    <dbReference type="eggNOG" id="arCOG01111">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015345_0_2_2"/>
    <dbReference type="OrthoDB" id="23397at2157"/>
    <dbReference type="PhylomeDB" id="Q97A04"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050242">
        <property type="term" value="F:pyruvate, phosphate dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.189.10">
        <property type="entry name" value="Pyruvate Phosphate Dikinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010121">
        <property type="entry name" value="Pyruvate_phosphate_dikinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004531">
        <property type="entry name" value="PRK05878.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01828">
        <property type="entry name" value="pyru_phos_dikin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE DIKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931:SF9">
        <property type="entry name" value="PYRUVATE, PHOSPHATE DIKINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000853">
        <property type="entry name" value="PPDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="12">
        <location>
            <begin position="20"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="12">
        <location>
            <begin position="63"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="12">
        <location>
            <begin position="302"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="11">
        <location>
            <begin position="418"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme C-terminal" evidence="13">
        <location>
            <begin position="518"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="746"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="770"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB60148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="886" mass="99665" checksum="89C78F9495658EF1" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A24</accession>
    <name>Q97A24_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1007935 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1007935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A24"/>
    <dbReference type="STRING" id="273116.gene:9381778"/>
    <dbReference type="PaxDb" id="273116-14325203"/>
    <dbReference type="KEGG" id="tvo:TVG1007935"/>
    <dbReference type="eggNOG" id="arCOG06977">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1044354_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="256" mass="27939" checksum="E8BC52EEC8980193" modified="2001-10-01" version="1">MIVNSLLKKATIIIVAVAVIVPAAYFAIQLNNKSSESSDPLYYVPSSSEAIGYINVNNTRAYFFYSNNSLALVINTGSMGGTLNFTGHLSDKSSSNKTSNLKLYESFMGNNIYMISNLSFSMYSFTIRNVTFYAYESNGFIVIGNLSAVKYSIEASIKGNNAISMHSSLNTSANISLYIHPLNSTVLKYAYANVSGQNLTATISFTNYTEESYFLSIILSFKSIKVREINNLTVKISGTLPSKGEIGNILGTLHAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A45</accession>
    <name>Q97A45_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oxygen-independent coproporphyrinogen III oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0989851</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_010917195.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A45"/>
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    <dbReference type="PaxDb" id="273116-14325182"/>
    <dbReference type="GeneID" id="1442043"/>
    <dbReference type="KEGG" id="tvo:TVG0989851"/>
    <dbReference type="eggNOG" id="arCOG01363">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_775993_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051198">
        <property type="entry name" value="BchE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
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        <property type="entry name" value="ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RADICAL SAM SUPERFAMILY PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01095">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="123"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="382" mass="43621" checksum="71773302B8E99C1B" modified="2001-10-01" version="1">MDTNGKIENGIISVEKDNKIVFTLDLSGRFLFYDNGDATYRRSLDNKFLRLKHTESGRSVELVDKEAGNKIADEAYNFLGSIAEDLPQELSIYARRFSSLNHEFLEEDAKKLHGIYGMSVPIVPPDQYFPVYIQAEKGCSWNRCTFCKLYRDRDYMVRLPDEFKEHVRQVKDYFGEGLSARKTVFLGDANAVNLEQKTLLSYLDLIKEEFGLPVYAFVDAISTQKRKSEMHFREMKDHGLKRVYLGLESGDPGVLRLLNKMMNLSEAINLVNQIKDAGLNIGIILMAGAGGRKFYENHVKNTASVISQMDLGRGDIIYISPIYEFDDADYYNISKGLGSLDDNEKEKQIGDLKTSIYETYQDMNGKKLEAPIVKYDLKEAIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A66</accession>
    <name>Q97A66_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0971163 protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">TVG0971163</name>
    </gene>
    <organism evidence="2 3">
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A66"/>
    <dbReference type="STRING" id="273116.gene:9381736"/>
    <dbReference type="PaxDb" id="273116-14325161"/>
    <dbReference type="KEGG" id="tvo:TVG0971163"/>
    <dbReference type="eggNOG" id="arCOG00645">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2748412_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="70" mass="7594" checksum="E8C7AD4980F1D51F" modified="2001-10-01" version="1">MPKVYIVGAGPGDPELLTLKAYNILRKAGVVIYAGSLVNPEILKYANPSAEIYNSAEMDINKIMDISIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A75</accession>
    <name>Q97A75_THEVO</name>
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        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0961784</name>
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    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="1442017"/>
    <dbReference type="KEGG" id="tvo:TVG0961784"/>
    <dbReference type="eggNOG" id="arCOG03649">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_20_3_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="PhylomeDB" id="Q97A75"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA_transporter"/>
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            <end position="61"/>
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        <location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAB60077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A93</accession>
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            <fullName evidence="2">TVG0944469 protein</fullName>
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            <taxon>Thermoplasmatota</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <feature type="transmembrane region" description="Helical" evidence="1">
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        </location>
    </feature>
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        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="193" mass="20983" checksum="6E917D00F275341F" modified="2001-10-01" version="1">MISPLAVMAVVILIASMHMIAPDHWMPLAALTLKKKYSDSKVRALSAYIGMAHGVSSVILSLAILYIGIAFIGSKYIRVASVIILVLVSLYILINSIRESREAREIEETSLLVSAFPDPAFLPMAIIAYTYGTYFVISTGLIYVAASTIALLAVVFLVNIGLLRKLSSLKPVTVDRIVVVVLLLTAAYIYFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AD6</accession>
    <name>Q97AD6_THEVO</name>
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        <submittedName>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="1441966"/>
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        <property type="component" value="Chromosome"/>
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    <dbReference type="Gene3D" id="3.30.70.1980">
        <property type="entry name" value="Uncharacterised protein PF09406, DUF2004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018546">
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09406"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="BAB60016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="123" mass="14856" checksum="7B2A5D2DFA3D83CA" modified="2001-10-01" version="1">MERGLEIAFQPVNGDDAKLAKALAAITANYFSDTMKVNYRIFLVELDGQHFFRILFESKELTKFHMEMRKKFMEQFDSVSKWSYEDVISYYEELLKDGRCKDIEIKRVKEEYDLWEDPIWKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AE4</accession>
    <name>Q97AE4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">2-phosphoglycerate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0885910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AE4"/>
    <dbReference type="STRING" id="273116.gene:9381658"/>
    <dbReference type="PaxDb" id="273116-14325083"/>
    <dbReference type="KEGG" id="tvo:TVG0885910"/>
    <dbReference type="eggNOG" id="arCOG01967">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412410_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AE4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008996">
        <property type="entry name" value="PRK12339.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477:SF3">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="208" mass="24141" checksum="E2608A19C2F875CA" modified="2001-10-01" version="1">MERGLMHSKIILIGGVPGVGKTSISGFLARELGIDIMLSGDYLREFLRPLFGDNPLIQKSVYDAWQAYGQKTDENIVKGYIDQAKIMMAGIDRILIRAIENGENLILETLYFLPEMLSEKARNNVHMFYLYIGDEKLHRDRLVDRINYTHKNSPGTRLAEHLYEYRNIMEYSMMRSSEYNVKIIDTSNYEEARRTILEMIVKGESRYA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AE6</accession>
    <name>Y864_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0173 metal-dependent hydrolase TV0864</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0864</name>
        <name type="ORF">TVG0885567</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0173 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917108.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AE6"/>
    <dbReference type="SMR" id="Q97AE6"/>
    <dbReference type="STRING" id="273116.gene:9381656"/>
    <dbReference type="PaxDb" id="273116-14325081"/>
    <dbReference type="GeneID" id="1441956"/>
    <dbReference type="KEGG" id="tvo:TVG0885567"/>
    <dbReference type="eggNOG" id="arCOG00497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070010_4_0_2"/>
    <dbReference type="OrthoDB" id="28313at2157"/>
    <dbReference type="PhylomeDB" id="Q97AE6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00457">
        <property type="entry name" value="UPF0173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022877">
        <property type="entry name" value="UPF0173"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050114">
        <property type="entry name" value="UPF0173_UPF0282_UlaG_hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001911">
        <property type="entry name" value="PRK00685.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF3">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13483">
        <property type="entry name" value="Lactamase_B_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156408" description="UPF0173 metal-dependent hydrolase TV0864">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00457"/>
        </source>
    </evidence>
    <sequence length="223" mass="24208" checksum="0F5F023CDDDA2E7C" modified="2001-10-01" version="1">MDVKIIWHGHACFSIEGKKNVLIDPFLTGNPMAKVKAEDLNPDIILVTHGHYDHAADAVSISKRTGAPVLSVFELSEIFKESGINTIDINPGGTVEFEGVSIKATIATHSSSYDGRYAGNPVGYVIDIGRKIYHAGDTGYFKDMELIGSVDRPEISLLPIGGHYTMDVDGAVEALKMLKSPIAIPMHYNTFDVIKADPLRFKNLAAAVGTYVIVPKVEEPVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AE7</accession>
    <name>Q97AE7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="2">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0884813</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917107.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AE7"/>
    <dbReference type="STRING" id="273116.gene:9381655"/>
    <dbReference type="REBASE" id="5143">
        <property type="entry name" value="M.TvoDam"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325080"/>
    <dbReference type="GeneID" id="1441955"/>
    <dbReference type="KEGG" id="tvo:TVG0884813"/>
    <dbReference type="eggNOG" id="arCOG03416">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063430_0_0_2"/>
    <dbReference type="OrthoDB" id="372040at2157"/>
    <dbReference type="PhylomeDB" id="Q97AE7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1020.10">
        <property type="entry name" value="Adenine-specific Methyltransferase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023095">
        <property type="entry name" value="Ade_MeTrfase_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012263">
        <property type="entry name" value="M_m6A_EcoRV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012327">
        <property type="entry name" value="MeTrfase_D12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00571">
        <property type="entry name" value="dam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30481">
        <property type="entry name" value="DNA ADENINE METHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30481:SF3">
        <property type="entry name" value="DNA ADENINE METHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02086">
        <property type="entry name" value="MethyltransfD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000398">
        <property type="entry name" value="M_m6A_EcoRV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00505">
        <property type="entry name" value="D12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="277" mass="32046" checksum="D894579F7476CBC3" modified="2001-10-01" version="1">MEYSKACCKPVLKWAGGKRQLIPYIMRYVPERFKTYYEPFLGGAAVLIHLYSKGRISKAVVSDVNRDLYMLYKEIKERPIELISVMRSLNFQNKREDYYRARDLFNETSDYKLRSALLIYLNKHGYNGLYRLNSLGLFNVPFGRHRNTSFPSDQDILSLSNMLSSCTILNEDFEKAVAGAESGDFVYFDPPYVPLSKTSNFTSYTESGFTHKDQVRLKDVFIELSNRGVFVMETNSDTENVRDLYKAFEIIGVPARRNINSNASRRNGATELIIRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AG0</accession>
    <name>Q97AG0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyruvate ferredoxin oxidoreductasse [POR] beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0872869</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917094.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AG0"/>
    <dbReference type="STRING" id="273116.gene:9381642"/>
    <dbReference type="PaxDb" id="273116-14325067"/>
    <dbReference type="GeneID" id="1441942"/>
    <dbReference type="KEGG" id="tvo:TVG0872869"/>
    <dbReference type="eggNOG" id="arCOG01601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058423_0_0_2"/>
    <dbReference type="OrthoDB" id="296931at2157"/>
    <dbReference type="PhylomeDB" id="Q97AG0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03376">
        <property type="entry name" value="TPP_PFOR_porB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051479">
        <property type="entry name" value="PorB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008818">
        <property type="entry name" value="PRK11864.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897:SF2">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="3">
        <location>
            <begin position="63"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="295" mass="33236" checksum="57D04DDDDFC706E1" modified="2001-10-01" version="1">MPISIPKQELMEPGHTACHGCGATLAMRYVLKALGTKTVVSVPASCWAVIPGAMPYRTLDVPMVYTPFAATGASISGLREALDIQGKEDYNVVGFAGDGGTADIGLQGLSGAMERGHNVFYIMYDNEGYMNTGVQRSGSTPYGAKTTTTPVGKERDFKRENKKVVADMMIAQNVPYVATATVAYPEDIIKKIERAREINGPKFIQILAPCPTGWYYPPEKTIEISRLAVQSRMFPLYEFVEGKFYLNKSFKQVDVTDYIKAQDRFRHLNDEEIEQLRRYIEERWNMLLEWEKLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AG5</accession>
    <name>Q97AG5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0869119 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0869119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917089.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AG5"/>
    <dbReference type="STRING" id="273116.gene:9381637"/>
    <dbReference type="PaxDb" id="273116-14325062"/>
    <dbReference type="GeneID" id="1441937"/>
    <dbReference type="KEGG" id="tvo:TVG0869119"/>
    <dbReference type="eggNOG" id="arCOG07387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079556_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="117" mass="13666" checksum="31469DB5306AF0AC" modified="2001-10-01" version="1">MEDIDSYLNYVNSIWVKINEKFKNQDENVKIAILQLAARPYYYWIQENLSPPPKKEGQETGKLENIVSKYGDSIQIENDTVKIVKRLGTTEFAALRDSLREIGYQYDAQSRGFKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AG8</accession>
    <name>Q97AG8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0863466</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917086.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AG8"/>
    <dbReference type="STRING" id="273116.gene:9381634"/>
    <dbReference type="PaxDb" id="273116-14325059"/>
    <dbReference type="GeneID" id="1441934"/>
    <dbReference type="KEGG" id="tvo:TVG0863466"/>
    <dbReference type="eggNOG" id="arCOG04531">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020088_6_0_2"/>
    <dbReference type="OrthoDB" id="211791at2157"/>
    <dbReference type="PhylomeDB" id="Q97AG8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF33">
        <property type="entry name" value="NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="423" mass="46166" checksum="0CB8D0C56B629E20" modified="2001-10-01" version="1">MDINIGTTHKVFHVLSKIGPAWIVMMADVDVASIITGLQSGAEFGFHMIFVMLILIPPLFIVQNAAGYLGIYSGMGVGEAIRQRYGRRLALLAAIPMASTDILSYIAEYSGIAIGLYMVGVSPYISVPVVFVIHNLIVLMRRYGRIESILMIVSVGLVLFIIVSVALMKPSWHSVILTGLNPIQPYGNHDFDIMIIANLGAIIMPFMIFYQAGASVQKSLLDQDIKISRNETAIGSIVSEVLMVMIVIAGTFIGGYATEPKMLAAALLPFGTYAYYITAAGFVTAGFLALIVISLASSWGIAEALGWKLYSGRRRGMHKAQMKGLTEERSDCDRRKFIYLYMGESIPAAAIALSFSAYLLPLVIDLMIVFVLVLAPVGVLLGLLVSDQKIMNGHRLSKRYMALFWFTLAIIEIAGLYSIITSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AI4</accession>
    <name>Q97AI4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-methylhydantoinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0835694</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="GeneID" id="1441918"/>
    <dbReference type="KEGG" id="tvo:TVG0835694"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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            <begin position="7"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="199"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="3">
        <location>
            <begin position="590"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="656" mass="72018" checksum="764DA9CB266A9DAB" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AJ5</accession>
    <name>Q97AJ5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0819318</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AJ5"/>
    <dbReference type="STRING" id="273116.gene:9381605"/>
    <dbReference type="PaxDb" id="273116-14325031"/>
    <dbReference type="GeneID" id="1441907"/>
    <dbReference type="KEGG" id="tvo:TVG0819318"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="PhylomeDB" id="Q97AJ5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF46">
        <property type="entry name" value="BLR6921 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="6"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="464" mass="50523" checksum="360D7CA6B8C1B1E1" modified="2001-10-01" version="1">MNRALILTTTSIGSFVTPFFSSVVSFAVPKIALFYHLNFQDASLIPLIILIPLASTMILLGRLADSIGRTFIFRIGFLIFSVSAFIGAFSKSFPILTVALFFSGFGSAALSTNSTALIGYIFAGDKRGSALGINAMAVYLGLTMAPFLGGILIEFVGWQSIFYLTAPLALIGLAVSFATLKGVEAKGRMNSDYLSAVFFSTALFSLAFFLALGYVYNFEQFLPLLIIGVAFLYLFFHRNKRVETQLIPANLFKSNRTFLFSNVTALLNYLSTFSIVFVFSIYLQVILKVSPFQAGLSILPEPVFMVILSPIAGRISDRIGSRYLASAGMAIIGLSFLFLYVFPKISVFLVMVFLSFIGIGFGFFSAPNTNSVMGSAPREMSGIASGFLGTMRFVGQFLSILVASFILSTFMPRTMVVGIFSGIFVTISQAYYDSFVSGFRLVMLISAVLSFIGAYTSLMKETGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AL8</accession>
    <name>Q97AL8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0793261 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0793261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
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                <person name="Makino K."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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</entry>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    </evidence>
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    </evidence>
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</entry>
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    <accession>Q97AM3</accession>
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            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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    </evidence>
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<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AP7</accession>
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            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917010.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AP7"/>
    <dbReference type="STRING" id="273116.gene:9381553"/>
    <dbReference type="PaxDb" id="273116-14324979"/>
    <dbReference type="GeneID" id="1441858"/>
    <dbReference type="KEGG" id="tvo:TVG0766521"/>
    <dbReference type="eggNOG" id="arCOG01434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_4_0_2"/>
    <dbReference type="OrthoDB" id="85597at2157"/>
    <dbReference type="PhylomeDB" id="Q97AP7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
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        <property type="entry name" value="PRK06450.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="4">
        <location>
            <begin position="52"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="340" mass="37877" checksum="FC2864128896433B" modified="2001-10-01" version="1">MPCIVRCIRCGNVRKNEELRCPVCGNLYEIYPDFKFQDDYEKNFPYIKKWVSLGEVETPIVNYGEVKFKIDYYQPTFSYKDRGSKVLISHIRDITPSQNISTISEDSSGNAGASIAAYGAAAGLKVKVYVPKTVSGQKFKQILVYGAEAVKIDGNREDVQIAAENSGYYYASHVLRPEFRDGIRSLAYEIFMQTEKMPDHIFIPVSAGTLLIGLYSGFEHLFRSGEIEKIPNIVAVQAEAVSPVCAKLNGEQFDENSNATSIADALVSKKPILLEKMLSILRENGKCIRVSDNEIIEARKELALKGIYAEYSSSTVFAAYKKKKLENSLLVLTGAGLKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AR6</accession>
    <name>Q97AR6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytochrome d ubiquinol oxidase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG0749124</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916990.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AR6"/>
    <dbReference type="STRING" id="273116.gene:9381533"/>
    <dbReference type="PaxDb" id="273116-14324959"/>
    <dbReference type="GeneID" id="1441838"/>
    <dbReference type="KEGG" id="tvo:TVG0749124"/>
    <dbReference type="eggNOG" id="arCOG02721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030555_3_2_2"/>
    <dbReference type="OrthoDB" id="41618at2157"/>
    <dbReference type="PhylomeDB" id="Q97AR6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070069">
        <property type="term" value="C:cytochrome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016682">
        <property type="term" value="F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002585">
        <property type="entry name" value="Cyt-d_ubiquinol_oxidase_su_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30365:SF14">
        <property type="entry name" value="CYTOCHROME BD MENAQUINOL OXIDASE SUBUNIT I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30365">
        <property type="entry name" value="CYTOCHROME D UBIQUINOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01654">
        <property type="entry name" value="Cyt_bd_oxida_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006446">
        <property type="entry name" value="Cyt_quinol_oxidase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB59885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="458" mass="51462" checksum="91C1454295B2D1E3" modified="2001-10-01" version="1">MNIGTFDRLLFAYSIGSHILIVLMSIGLSVILPVLEYIAIKYKDVYYDLLTRRVAKALVMFFAVGTASGAVMAVELIDLFPKFMTIIAVVGVLPFYYEIFAFFLETIMLVSYYYYWDVFKNRMHHWLLTFGVAGGTLASAVFITMINAWMNTPNGFNISYYEKTGKLIDVNPFITALTPSTGREVFHVVIVTAFAGLMVLGAFFAYQYLRAKTEEAKTLYMKGLKVTFPMNMFLIIMAGLSGTLEMSGLLTYQPEKYAALDLNPYPIARDAPEHLFGTIVEKNGHYYVLGGIKIPFVQSLLAGNGNLNTPVPGLSQFPTSTWPPLAVHDTFDLMVIGGTLLGLFLLSVLIAWILKKKPYESKGFMVAQILFAILTIPIYIGGWVTDEVGRQPWIIYNVMTVYQAANYSPSIIVPGYLIMAFYTILMPFSYWYAFRLMKQGDVKKEISSYKMPSTAHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AR8</accession>
    <name>Q97AR8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0744794</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916988.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AR8"/>
    <dbReference type="STRING" id="273116.gene:9381531"/>
    <dbReference type="PaxDb" id="273116-14324957"/>
    <dbReference type="GeneID" id="1441836"/>
    <dbReference type="KEGG" id="tvo:TVG0744794"/>
    <dbReference type="eggNOG" id="arCOG01991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_9_3_2"/>
    <dbReference type="OrthoDB" id="304253at2157"/>
    <dbReference type="PhylomeDB" id="Q97AR8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054929">
        <property type="entry name" value="dPGM_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038349">
        <property type="entry name" value="dPGM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="189" mass="21578" checksum="988FBC22B6DA713F" modified="2001-10-01" version="1">MNRAILVRHGESETNLYGIISSDYDRYPLTQYGREQVTFIAPKLAELKFDGIITSPILRAVQTARIIANKCGLEILTDDRVRESGFGPYNNMHISEIPWKSREDMKMEPWESHVKRMREVINEYDGSYIIVSHAYPIKALICDMLGMGEDDCYSVEIKNASMSAIDVTNGRVLSIGTFLISDRVKRFFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AR9</accession>
    <name>AROC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chorismate synthase</fullName>
            <shortName evidence="1">CS</shortName>
            <ecNumber evidence="1">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroC</name>
        <name type="ordered locus">TV0740</name>
        <name type="ORF">TVG0743399</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="1">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chorismate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AR9"/>
    <dbReference type="SMR" id="Q97AR9"/>
    <dbReference type="STRING" id="273116.gene:9381530"/>
    <dbReference type="PaxDb" id="273116-14324956"/>
    <dbReference type="KEGG" id="tvo:TVG0743399"/>
    <dbReference type="eggNOG" id="arCOG04133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034547_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AR9"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="entry name" value="Chorismate synthase AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003793">
        <property type="entry name" value="PRK05382.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085:SF0">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="Chorismate synthase, AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140700" description="Chorismate synthase">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="296"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <sequence length="366" mass="40029" checksum="70762801FC4FA08A" modified="2001-10-01" version="1">MVMFYLGNVLKLSLFGSSHGESVGSLVDGFPVGYKIPLDYIQKYMEYRRPGNTIITSQRKEEDVVKIISGMHNGFTDGSPITMIIENKNVISSYYEELKENPRPGHSDYTLYVKYGDFRNYEGGGFLSGRMTAPLVAAGALAKSYLDSLGIKITSYMKSIGEVTCDEIRGDPYNFETRIPDPEKNSLATSLLVDIMKSGDSIGGRIDTIVEGLPPGVGEPFFNSVESEISRAMFSIPAVKAIEFGDGFTLSKMKGSEANDPFTVLDGHILTKTNHNGGILGGMTTGMPVKFSIAVKPTPSIHKKQDTINLISKEKSEITVKGRHDPCVAIRAVPVVECLTAFVLMDLMMMGGFIDRYGNVRGRSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AS0</accession>
    <name>Q97AS0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glucose kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0742429</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916986.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AS0"/>
    <dbReference type="STRING" id="273116.gene:9381529"/>
    <dbReference type="PaxDb" id="273116-14324955"/>
    <dbReference type="GeneID" id="1441834"/>
    <dbReference type="KEGG" id="tvo:TVG0742429"/>
    <dbReference type="eggNOG" id="arCOG04280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_0_4_2"/>
    <dbReference type="OrthoDB" id="206224at2157"/>
    <dbReference type="PhylomeDB" id="Q97AS0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049874">
        <property type="entry name" value="ROK_cs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964:SF149">
        <property type="entry name" value="BIFUNCTIONAL UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE_N-ACETYLMANNOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="ROK (REPRESSOR, ORF, KINASE) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="315" mass="33823" checksum="681AAAEF463D9734" modified="2001-10-01" version="1">MALKILGYDVGGTKISTVVGNDNGNILSNVRMPTVKHLGKKRLIEEMITMGDAALKKAGVDKPDMIGIIFAGPVDNKKGVVIASPNIFGLSNFNIVEPIQKYYGAKTYLENDATAAAIAERLFGSGKNVDNFVYMTLSTGIGGGIFINGKLYKGAHGMAGELGHSVIMVNGPTCGCGRRGCLEALAGGKAIARRVIENLNAVRDSNFFSRIRPTEITAEKVFEGKKKGDMFSQLILEETIYYLAVGIVNIINILDPELVIIGGGISKAGKDLFDPLNDAIREEFKSMYRPFKIVPGLENGSDLAAISVPLYKESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AS6</accession>
    <name>Q97AS6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0738831</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053979.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AS6"/>
    <dbReference type="STRING" id="273116.gene:9381522"/>
    <dbReference type="PaxDb" id="273116-14324949"/>
    <dbReference type="GeneID" id="24779930"/>
    <dbReference type="KEGG" id="tvo:TVG0738831"/>
    <dbReference type="HOGENOM" id="CLU_2911749_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="61" mass="7117" checksum="909EA8EB5C22F19F" modified="2001-10-01" version="1">MGLVEKVITLNKKLNQFNGKKTDENYEILDEIKRVDTQIDIAIYRLYGLTAEERKVIEESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AS7</accession>
    <name>Q97AS7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0738178 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0738178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053978.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AS7"/>
    <dbReference type="STRING" id="273116.gene:9381521"/>
    <dbReference type="PaxDb" id="273116-14324948"/>
    <dbReference type="GeneID" id="24779929"/>
    <dbReference type="KEGG" id="tvo:TVG0738178"/>
    <dbReference type="HOGENOM" id="CLU_1507466_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="178" mass="20418" checksum="A68E80CC1DF5AE47" modified="2001-10-01" version="1">MGDYCKYCGSSDLEKVKYEAKYTNSTKTSKHIGLSHIIKAGVDNSSEGKIENTLYIDTYHCKKCKKIDCLCNIEPLEISISGDYEYKIIIPDAFQELLKNFHVDEIKLKNLISNLVNQRGIKKNINDFITNGNNKYKNNMIGFHHKLKIDNRDISINILMEMKDGMLLRIIYDGGKSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AV6</accession>
    <name>SFSA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sugar fermentation stimulation protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sfsA</name>
        <name type="ordered locus">TV0703</name>
        <name type="ORF">TVG0708898</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SfsA family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59845" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59845.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053969.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AV6"/>
    <dbReference type="SMR" id="Q97AV6"/>
    <dbReference type="STRING" id="273116.gene:9381492"/>
    <dbReference type="PaxDb" id="273116-14324919"/>
    <dbReference type="GeneID" id="1441810"/>
    <dbReference type="KEGG" id="tvo:TVG0708898"/>
    <dbReference type="eggNOG" id="arCOG04115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052299_1_0_2"/>
    <dbReference type="OrthoDB" id="34139at2157"/>
    <dbReference type="PhylomeDB" id="Q97AV6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22357">
        <property type="entry name" value="SfsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00095">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005224">
        <property type="entry name" value="SfsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040452">
        <property type="entry name" value="SfsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041465">
        <property type="entry name" value="SfsA_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00230">
        <property type="entry name" value="sfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545">
        <property type="entry name" value="SUGAR FERMENTATION STIMULATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545:SF2">
        <property type="entry name" value="SUGAR FERMENTATION STIMULATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03749">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17746">
        <property type="entry name" value="SfsA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152331" description="Sugar fermentation stimulation protein homolog">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="216" mass="24748" checksum="BE8039A4CA4F711F" modified="2001-12-05" version="2">MEFTDLLPCTVVERVNRFLVNVKLNDKIVEAHLHDPGRLKEIIYTGNKVLVRRKSGKKTGYRITFGLREDQYILIDSGLHSQIASHFVSQECKPEVKIDDRRLDFACNDIFIEVKGCTLSIDGVAIFPDAPTLRGYEHLRLLERLAQEGKGAYVLFLIFSDATSFRPNSETDPRFSDEFYKALKNGVKFSFKRFSFDGKYLKYSGDILTFDGDDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AX6</accession>
    <name>Q97AX6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0687568 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0687568</name>
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    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053953.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AX6"/>
    <dbReference type="PaxDb" id="273116-14324899"/>
    <dbReference type="GeneID" id="1441789"/>
    <dbReference type="KEGG" id="tvo:TVG0687568"/>
    <dbReference type="HOGENOM" id="CLU_1656981_0_0_2"/>
    <dbReference type="OrthoDB" id="58081at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="159" mass="18984" checksum="84D4CEF1FAB811E0" modified="2001-10-01" version="1">MASIGIHTQIAFCEIAIRNFVRFKNPYKLDFGEKFLTDLKKSPGADIKIDRAEGRFENKKRSQTETDLFDELDFDEELILFREIMKPNLDANEKAHFEKFVNLEDSRIEFIKNMRNEVMHSKPEIIKKHSDINEWLKFLQDCQYFIRTIEDNTFAPFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B09</accession>
    <name>Q97B09_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alkaline phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0645996</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B09"/>
    <dbReference type="STRING" id="273116.gene:9381438"/>
    <dbReference type="PaxDb" id="273116-14324866"/>
    <dbReference type="DNASU" id="1441757"/>
    <dbReference type="KEGG" id="tvo:TVG0645996"/>
    <dbReference type="eggNOG" id="arCOG03117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_1_1_2"/>
    <dbReference type="OrthoDB" id="204088at2157"/>
    <dbReference type="PhylomeDB" id="Q97B09"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051311">
        <property type="entry name" value="DedA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709">
        <property type="entry name" value="ALKALINE PHOSPHATASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709:SF6">
        <property type="entry name" value="UNDECAPRENYL PHOSPHATE TRANSPORTER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="7">
        <location>
            <begin position="86"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="261" mass="29296" checksum="44F9595279998BC6" modified="2001-10-01" version="1">MGISVASSNKPNNYDRYFNIYGVVIAAVLAAIAAIEIFEIFELPFEKYFAGIDIASIFSFTTKVVSDSSYVGIFILMTLESMFIPIPSEVILPLSGFLVYQGSFNFAVVLADTIIASMVGSFIDYFLGLYLGRPIIVKILSYFSISEDSLSRAETWIDKMGSFSVFLARFIPGIRSLISLPAGMLKMKMWLFALMTFLGSFIWSFSLIYLGYSAGPYWSVAVKNFSALLDQIIIDVVFLASLAYIFYYIFLKIKRRQHALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B24</accession>
    <name>Q97B24_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0628554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916894.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B24"/>
    <dbReference type="STRING" id="273116.gene:9381423"/>
    <dbReference type="PaxDb" id="273116-14324851"/>
    <dbReference type="GeneID" id="1441742"/>
    <dbReference type="KEGG" id="tvo:TVG0628554"/>
    <dbReference type="eggNOG" id="arCOG04048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087476_3_1_2"/>
    <dbReference type="OrthoDB" id="33242at2157"/>
    <dbReference type="PhylomeDB" id="Q97B24"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680:SF3">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22769">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="157" mass="17630" checksum="43BD42DD720B2922" modified="2001-10-01" version="1">MVLNDNQIQKMVEQGFLISENFSDACLTPNGYDVRVESILADEKEVDETIVGTMKHFLVSTLEVLRLPENVIANIWIRSSYARRGVIGSFGTIDAGYHGSLTLSFFNAGPDLHISRGDRIAQVIFLEMKEKSSMSYEMRSGHYQHSRGVVKGEDKDI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B28</accession>
    <name>PYRI_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate carbamoyltransferase regulatory chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrI</name>
        <name type="ordered locus">TV0631</name>
        <name type="ORF">TVG0624900</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in allosteric regulation of aspartate carbamoyltransferase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Contains catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PyrI family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916890.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B28"/>
    <dbReference type="SMR" id="Q97B28"/>
    <dbReference type="STRING" id="273116.gene:9381419"/>
    <dbReference type="PaxDb" id="273116-14324847"/>
    <dbReference type="GeneID" id="1441738"/>
    <dbReference type="KEGG" id="tvo:TVG0624900"/>
    <dbReference type="eggNOG" id="arCOG04229">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128576_0_0_2"/>
    <dbReference type="OrthoDB" id="7000at2157"/>
    <dbReference type="PhylomeDB" id="Q97B28"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009347">
        <property type="term" value="C:aspartate carbamoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.20">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.140">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00002">
        <property type="entry name" value="Asp_carb_tr_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020545">
        <property type="entry name" value="Asp_carbamoyltransf_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002801">
        <property type="entry name" value="Asp_carbamoylTrfase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036793">
        <property type="entry name" value="Asp_carbatrfase_reg_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00240">
        <property type="entry name" value="ATCase_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805:SF1">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01948">
        <property type="entry name" value="PyrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54893">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000142346" description="Aspartate carbamoyltransferase regulatory chain">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00002"/>
        </source>
    </evidence>
    <sequence length="152" mass="17388" checksum="6EBE7584256E1373" modified="2001-10-01" version="1">MEEKTLKISKIKDGTVIDHIPSGKALRVLSILGIRDDVDYTVSVGMHVPSSKMEYKDVIKIENRSLDKNELDMISLTAPNATISIIKNYEISEKFKVELPPKLIGIIKCKNQNCITNTREPVKPEFEIVSRHPLVLRCVYCQRTMNERDVFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B29</accession>
    <name>PYRB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate carbamoyltransferase catalytic subunit</fullName>
            <ecNumber evidence="1">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartate transcarbamylase</fullName>
            <shortName evidence="1">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrB</name>
        <name type="ordered locus">TV0630</name>
        <name type="ORF">TVG0624440</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterooligomer of catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916889.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B29"/>
    <dbReference type="SMR" id="Q97B29"/>
    <dbReference type="STRING" id="273116.gene:9381418"/>
    <dbReference type="PaxDb" id="273116-14324846"/>
    <dbReference type="GeneID" id="1441737"/>
    <dbReference type="KEGG" id="tvo:TVG0624440"/>
    <dbReference type="eggNOG" id="arCOG00911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_1_2_2"/>
    <dbReference type="OrthoDB" id="7792at2157"/>
    <dbReference type="PhylomeDB" id="Q97B29"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000001">
        <property type="entry name" value="Aspartate carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF6">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113262" description="Aspartate carbamoyltransferase catalytic subunit">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <sequence length="305" mass="34219" checksum="BE22327483C5D434" modified="2001-10-01" version="1">MLKNKSVVSIDDVQNDDLFEIFDLSESMLKTIESKGNAPLLTNKIMATLFYEPSTRTRLSFESAMHRLGGSVITVSDTKTSSAAKGETLADTVRMASSYSDIIVIRHPLEGAARLASRFTSKPIINAGDGSGQHPTQTILDLFTIKKELGSIDGKVITMVGDLRYGRTIHSLIIALSRFDVKVNLVSPEILRLPEYVYSMLPDRNYVKEYNDLEEVIGETDVLYVTRIQKERFSDQNEYQSVIGSYSINSETVEKMKKKSVIMHPLPRVDEISPDVDNMPQAAYFRQAYYGVPVRMALIYKILGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B65</accession>
    <name>Q97B65_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heterodisulfide reductase chain D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0581547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916851.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B65"/>
    <dbReference type="STRING" id="273116.gene:9381381"/>
    <dbReference type="PaxDb" id="273116-14324808"/>
    <dbReference type="GeneID" id="1441699"/>
    <dbReference type="KEGG" id="tvo:TVG0581547"/>
    <dbReference type="eggNOG" id="arCOG00332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_2_1_2"/>
    <dbReference type="OrthoDB" id="42878at2157"/>
    <dbReference type="PhylomeDB" id="Q97B65"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF2">
        <property type="entry name" value="HETERODISULFIDE REDUCTASE RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich" evidence="1">
        <location>
            <begin position="202"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="300" mass="33777" checksum="BBE1F46F14EADF47" modified="2001-10-01" version="1">MDEDYVKKQEATIRNVLVKNLMESYVPFPLDKKIATQWAYAINVPRGGSTIIYTSYMYQMANVFKSYEKYVPTFGSLGSSKIIASIGAKLIKPKEEDIKRFNAILQNIYRIVKRSNENIGYLYEEEPYSGSLLYELGFMDEFKEYGKKVFELFKQHKVSNIITIDPHTTNTLTNLKKYIGFDIPFTPYLNLIKEAKGTGKFVLHDSCLYSRFLGMYESIRTTIRSAGVELVEDPTVTGKGAGFCCGGPVGPLNDKLSNEIAKARAETLTSVNKDVLVACPLCYANLSEFCNVKDIAEVIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-15" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B99</accession>
    <name>RADB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>DNA repair and recombination protein RadB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">radB</name>
        <name type="ordered locus">TV0558</name>
        <name type="ORF">TVG0547169</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RecA-like protein family. RadB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B99"/>
    <dbReference type="SMR" id="Q97B99"/>
    <dbReference type="STRING" id="273116.gene:9381343"/>
    <dbReference type="PaxDb" id="273116-14324773"/>
    <dbReference type="KEGG" id="tvo:TVG0547169"/>
    <dbReference type="eggNOG" id="arCOG00417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041732_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q97B99"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011939">
        <property type="entry name" value="DNA_repair_and_recomb_RadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02237">
        <property type="entry name" value="recomb_radB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF47">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RADB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003336">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150121" description="DNA repair and recombination protein RadB">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="234" mass="26009" checksum="34D543510B0DB231" modified="2001-10-01" version="1">MMEEISLQEKIEKLPLGVACIDGLTNGGLEAGIITEIFGEGGSGKSNVCMLAACSALRLGKKVVFIDSEGFSSERFLSICKADNSKLKVIRVFSLDDQEVAITKFSKIIEKDASYGLFILDSFSEFFRLERTGDNQSRVIDFQRQLSLLSSIAAKRKIPVLITNQIYQDIANGDILPFGGYVVDHIMKAIYRLERLPDGRRKMTVMKHRSVKEGNSVEFRIVQDGISCEVKNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BA0</accession>
    <name>Q97BA0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">XAA-pro dipeptidase [X-pro peptidase]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0545002</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916816.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BA0"/>
    <dbReference type="STRING" id="273116.gene:9381342"/>
    <dbReference type="PaxDb" id="273116-14324772"/>
    <dbReference type="GeneID" id="1441074"/>
    <dbReference type="KEGG" id="tvo:TVG0545002"/>
    <dbReference type="eggNOG" id="arCOG01000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_0_2"/>
    <dbReference type="OrthoDB" id="1346at2157"/>
    <dbReference type="PhylomeDB" id="Q97BA0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01092">
        <property type="entry name" value="APP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050659">
        <property type="entry name" value="Peptidase_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112:SF2">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="5">
        <location>
            <begin position="28"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="4">
        <location>
            <begin position="140"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="360" mass="39833" checksum="43D6506818560365" modified="2001-10-01" version="1">MIFDKIFEYAREADTVLIYNAGEQNRDPTFTYITQTTKGIFEGSALIVKKDKATIVTSQLEEEAARSTGMDVYIFKNGTEFQNILRDLLKDVNTVGLNYNSLSLSSYKMFLRIIPDKEFLDVSASILEARKVKNEEELKKLKEAAKIGSEILPDVLNDLKEGVTEYEVASKIVYYMMRNGASGPSFDTIVAFGQNASMPHYSPGKAKLKKGDFVLMDYGAKYEGYCSDITRTVVFGKASEEQKEMYYTVKKAQEAGMNAIRSGANGKDVDAAARNVIDSTKYKGRFIHSLGHGVGLEVHDHPALSPSLDLPLKESMVVTVEPGVYVPGVGGVRIEDDVVVKKDGFEKITSAPTYELIEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BD3</accession>
    <name>Q97BD3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-NAD kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0513972</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916781.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BD3"/>
    <dbReference type="STRING" id="273116.gene:9381307"/>
    <dbReference type="PaxDb" id="273116-14324738"/>
    <dbReference type="DNASU" id="1441039"/>
    <dbReference type="GeneID" id="1441039"/>
    <dbReference type="KEGG" id="tvo:TVG0513972"/>
    <dbReference type="eggNOG" id="arCOG01350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064691_0_0_2"/>
    <dbReference type="OrthoDB" id="56451at2157"/>
    <dbReference type="PhylomeDB" id="Q97BD3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039065">
        <property type="entry name" value="AcoX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011386">
        <property type="entry name" value="Put_ATP-NAD_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697">
        <property type="entry name" value="ACETOIN CATABOLISM PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697:SF2">
        <property type="entry name" value="ATP-NAD KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016907">
        <property type="entry name" value="Kin_ATP-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="38409" checksum="B3CDB1CF80805111" modified="2001-10-01" version="1">MNIAFVINPVAGLGATLNLKGSDGLALEDVSKSISIVRSHRFLKKLDFNGLHFFVPSGCMGESEVSIYTKEYDVIYTPSSPTTRQDTINFVKSIESADLLVFVGGDGTARDILNSRNDLPVIGVPAGVKMYSSVFAISPERAADAVNQVAKGNYRIDKAEVVDIDEELYRKGILSVKRYGQLKVPEAQYIVENSKAEYDIAGAEDIAEYVIEHMADETYIIGPGNTCKTIEEEMGIKSNVLGFDILRNKKLIKEDASESEIYEACSDKCMLVISPVGGQNFLLGRGNKQISDRILERVGEENLIVVSSPSKAEHLKYVYIDTQIPLWSNGYVKVLVGYGRYKLVKVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BE5</accession>
    <name>Q97BE5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transport system ATP-binding protein A1A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0500467</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916769.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BE5"/>
    <dbReference type="STRING" id="273116.gene:9381294"/>
    <dbReference type="PaxDb" id="273116-14324726"/>
    <dbReference type="GeneID" id="1441027"/>
    <dbReference type="KEGG" id="tvo:TVG0500467"/>
    <dbReference type="eggNOG" id="arCOG00181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="OrthoDB" id="56961at2157"/>
    <dbReference type="PhylomeDB" id="Q97BE5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067:SF3">
        <property type="entry name" value="MALTOSE ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067">
        <property type="entry name" value="OLIGOPEPTIDE/DIPEPTIDE ABC TRANSPORTER, ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="9"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="306" mass="34663" checksum="D97BEECBF7D24F3E" modified="2001-10-01" version="1">MNEEILPILTVKDLNAGYLTNEKYIGIIHNINIELNRGKIVGITGLSGSGKTTLLKSILLETHIKSGEIIFNGKPIMPGDTKNYRRSVIYVPQNSLDSLDIIWPIEDQVKKILKQHNVVYDINKVSEIAKLIDLDISMLKLKSFEVSYGTRHKIVLLMSILMAKRDLVVLMDEPTLSQDAITAEGILNIIVKLAKDYGSSFIIVSSSPEPVFYASDFIYIMYGGWIVENGTPNDLSKWPKHPFTYEFINMLPAHKTRDRFPKFPMTEFSENGCPYSIYCKNKIKGCDSHINMFGEGHKYRCLNPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BE9</accession>
    <name>Q97BE9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Archaeal chaperonin [group II]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0494466</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916765.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BE9"/>
    <dbReference type="STRING" id="273116.gene:9381290"/>
    <dbReference type="PaxDb" id="273116-14324722"/>
    <dbReference type="GeneID" id="1441023"/>
    <dbReference type="KEGG" id="tvo:TVG0494466"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="OrthoDB" id="9362at2157"/>
    <dbReference type="PhylomeDB" id="Q97BE9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="522"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="523"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="544" mass="58483" checksum="36D1E318CD91AC4C" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BG2</accession>
    <name>Q97BG2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0479984</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CGI121/TPRKB family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BG2"/>
    <dbReference type="STRING" id="273116.gene:9381276"/>
    <dbReference type="PaxDb" id="273116-14324709"/>
    <dbReference type="KEGG" id="tvo:TVG0479984"/>
    <dbReference type="eggNOG" id="arCOG02197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152336_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2380.10">
        <property type="entry name" value="CGI121/TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013926">
        <property type="entry name" value="CGI121/TPRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036504">
        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011465">
        <property type="entry name" value="PRK14886.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
        <property type="entry name" value="CGI-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143870">
        <property type="entry name" value="PF0523-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="145" mass="16357" checksum="97D638A21CB20382" modified="2001-10-01" version="1">MLGRMSHKVVYFKIKYKIDINKIVGFIRSTKNLFQLIDGRCLLSQTQILSAISRAGRRYIKGSRVNNEGILILMFVSASTQIDSAISSCGLKPDSSTAALIYDDESDYSNFIAKFPEFVMVDPFVPYDVKDDSIFEKMSYVDATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BG5</accession>
    <name>Q97BG5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0475661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916749.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BG5"/>
    <dbReference type="STRING" id="273116.gene:9381272"/>
    <dbReference type="PaxDb" id="273116-14324705"/>
    <dbReference type="GeneID" id="1441007"/>
    <dbReference type="KEGG" id="tvo:TVG0475661"/>
    <dbReference type="eggNOG" id="arCOG04702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083918_3_2_2"/>
    <dbReference type="OrthoDB" id="28720at2157"/>
    <dbReference type="PhylomeDB" id="Q97BG5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00865">
        <property type="entry name" value="PEBP_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005247">
        <property type="entry name" value="YbhB_YbcL/LppC-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00481">
        <property type="entry name" value="YbhB/YbcL family Raf kinase inhibitor-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289:SF1">
        <property type="entry name" value="PEBP (PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="148" mass="16759" checksum="F47817D49E213DE9" modified="2001-10-01" version="1">MKISIKDFENESSIPTKFTCDGEDEMPVLSVTDVPKEAKCLALVVDDPDAPSGLFTHCIIYDIPLSKMEIDSSILNEKGVKAGINDFGEKGYRGPCPPPGKPHRYYFTVYALNTEIKEDGLKRREFDKEIKNHIIEKVSYMGTYIRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BL0</accession>
    <name>Q97BL0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0435626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BL0"/>
    <dbReference type="STRING" id="273116.gene:9381225"/>
    <dbReference type="PaxDb" id="273116-14324660"/>
    <dbReference type="DNASU" id="1440963"/>
    <dbReference type="KEGG" id="tvo:TVG0435626"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_707174_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BL0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="HTH bat-type" evidence="2">
        <location>
            <begin position="326"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="390" mass="46339" checksum="A5C7ED8401546813" modified="2001-10-01" version="1">MENFRKFVWESICSLQYYITHVFLPFLVYFSIKEVVHNGRNITIFPVSVYIVIIYKQRVITYPSLFKFCYHFIPNASMLFDIFCFSCTIYFQCKKVPHSLNVNSILIYIIIVNTKINDTVKILYKVLIFNDICIFLKDIIMLIHIYFDHMLAMNSRTSFVSPDDRFSMVYFKMEHEGCWTGITKEHNIAIHTLKIIPLKDRNNIYGLFEIRAQGKKEFRDFLRRINREKTIHRVMAYSPSELRKNVYFIDLYEVYGGMIEGILNDYQSIFEYDVVRRGVEEKYAIIPSDRVLELKNSINGLGKIYQFKARCIKNFYYAFSPFLSYTPSEMQIIAEAVSRGYYSIPRHVGIRDLAEYFGMSKSTVQEYLRKAEQKTMNNLKLLKLMNEMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BL3</accession>
    <name>Q97BL3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type II methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">N-4 cytosine-specific methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0430782</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916700.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BL3"/>
    <dbReference type="STRING" id="273116.gene:9381221"/>
    <dbReference type="REBASE" id="5141">
        <property type="entry name" value="M.TvoI"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324657"/>
    <dbReference type="DNASU" id="1440958"/>
    <dbReference type="GeneID" id="1440958"/>
    <dbReference type="KEGG" id="tvo:TVG0430782"/>
    <dbReference type="eggNOG" id="arCOG00129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063228_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0680">Restriction system</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="4">
        <location>
            <begin position="124"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="4">
        <location>
            <begin position="221"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="347" mass="39774" checksum="961DF9B4E4E282E1" modified="2001-10-01" version="1">MIDSSLVLDAETIRNFVKSVNDADSMDKVRAAVSSFAEELRSKSSENGISIREDVLKSILQRIASSHTTELARHYANEVYNMAFISRKLPYSDIDLSRWREYDFVITDSLWLFDKRDYRGSKLGWYWGNFVPQIPRQLILRFSRKDEWVLDPFSGSGTTLIEAKKLGRNSLGIEINEEVCKKSLEILNSIDGDGFSTAISGDSASVNLTKVMEYYGIPEFNLVIMHPPYHDIIKFTDIGGDLSNARDTKEFLSMLGKVTRNVSKYLQKGRFLALVIGDKYSNGEWIPLGFYSMQKVMDQGFRLKSTIVKNFEYTRGKASSSDLWRYRALAGGFYVFKHEYIFVFQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BM3</accession>
    <name>Q97BM3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Plant-type L-asparaginase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0422264</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916690.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BM3"/>
    <dbReference type="STRING" id="273116.gene:9381211"/>
    <dbReference type="MEROPS" id="T02.002"/>
    <dbReference type="PaxDb" id="273116-14324647"/>
    <dbReference type="GeneID" id="1440948"/>
    <dbReference type="KEGG" id="tvo:TVG0422264"/>
    <dbReference type="eggNOG" id="arCOG04779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021603_1_2_2"/>
    <dbReference type="OrthoDB" id="18230at2157"/>
    <dbReference type="PhylomeDB" id="Q97BM3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04703">
        <property type="entry name" value="Asparaginase_2_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF13">
        <property type="entry name" value="ISOASPARTYL PEPTIDASE_L-ASPARAGINASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="188"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="210"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="4">
        <location>
            <begin position="160"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00044776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="274" mass="29692" checksum="0F78F3E446744F08" modified="2001-10-01" version="1">MRNVLLIHGGAGSDPSYSGRLNSYASFASEEISALDNAVKAVVKMEDDPDFNAGTGSVPRLDGSIQMDAAVMIPGHFGSVINIERVKNPVLVARDVMAKSPHIMISGDGAVKFARLMGYPDYDPTTEKAKKLLQQFEEEAEKNEIPDKFKIYFKYVKPHDTVGAVARVEGEFAGAVSTGGSFPMVRGRVGDSPIPGAGIYVGEKGAVVATGIGEEIAKNILSFRIYNRIGEKRLYDIVKEEVDKFSVSVGIIAVDEKDYAYYANRPMAVGIKEF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-13" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BN3</accession>
    <name>RL10_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acidic ribosomal protein P0 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl10</name>
        <name evidence="1" type="synonym">rplP0</name>
        <name type="ordered locus">TV0422</name>
        <name type="ORF">TVG0407450</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916679.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BN3"/>
    <dbReference type="SMR" id="Q97BN3"/>
    <dbReference type="STRING" id="273116.gene:9381200"/>
    <dbReference type="PaxDb" id="273116-14324637"/>
    <dbReference type="GeneID" id="1440937"/>
    <dbReference type="KEGG" id="tvo:TVG0407450"/>
    <dbReference type="eggNOG" id="arCOG04288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053173_0_0_2"/>
    <dbReference type="OrthoDB" id="30930at2157"/>
    <dbReference type="PhylomeDB" id="Q97BN3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00280">
        <property type="entry name" value="Ribosomal_uL10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022909">
        <property type="entry name" value="Ribosomal_uL10_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003100">
        <property type="entry name" value="PRK04019.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000154810" description="Large ribosomal subunit protein uL10">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="314" mass="34343" checksum="6A6B27CD565A64CA" modified="2001-10-01" version="1">MRKINPKKKEIVSELAQDITKSKAVAIVDIKGVRTRQMQDIRAKNRDKVKIKVVKKTLLFKALDSINDEKLTKLKDATTGQIAVLTSQLDPTEIYQMVQATLTKASPRGGEVAPADITVEPMVTGFPPGPMMTEFQKVGLQTGVEKGKIAIKKEAVLVKKGEVIPKDKAKIMAMLDIKPLEVGLELLGLYSDGVLYSADVLSLTPEKIAEQMAIGYAQALQISKASMFFVAEVLKDLIAEAKVKADAFAVAAQFITEENLKEFLAIANRNAIILNNELNKGNTTEKESEKVEEPKEAEKSVDESISEGLGSLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BQ6</accession>
    <name>Q97BQ6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Betaine aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0388541</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916655.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BQ6"/>
    <dbReference type="STRING" id="273116.gene:9381177"/>
    <dbReference type="PaxDb" id="273116-14324614"/>
    <dbReference type="GeneID" id="1440913"/>
    <dbReference type="KEGG" id="tvo:TVG0388541"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_2"/>
    <dbReference type="OrthoDB" id="6342at2157"/>
    <dbReference type="PhylomeDB" id="Q97BQ6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000012">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="498" mass="54569" checksum="1B242F7A720738AC" modified="2001-10-01" version="1">MINTENFGNIINGTEEMVGDESIVKSPVDGSPIASVYFADRDAVSKAIDSSYDSYYKVWSKFTLSERKKLLAKLADRIQEKSERYATLESLNTGKTLRQSMLMDIPLGIEHLRYFATETEFKMERQITHPEYPDSHGIVQYVPMGVVGAITPWNVPFLMAVWKAAPALLAGNTVVIKPSSFTPLTTIEMARDAISVGFPPGVLNVVNGRGETVGEELARNKKVRMLSFTGSTYTGKRITEITGIKKVTLELGGKSPNIVFDDADLERAALGVMFGIYLNSGQLCESGSRLLVQSSIKDKFLNLLKSKIEKMRPGNPMSMETDISAITTKNQRDKIEKMVNSGLKDGSVIFYQKSIDGSVPKGGLYFPPTILDKVSEDMEVAKEEIFGPVLSVMEFDDEADAIDKANMTDYGLAAGVWTQDSEKATRVASSIEAGTVWINEYHLLSAAAPRGGFKNSGVGRELGLDGIMEFTQTRHLFVSHGSTDQDTVAYGLVLADLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BS4</accession>
    <name>Q97BS4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-responsive regulatory protein [Lrp]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0368274</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916635.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BS4"/>
    <dbReference type="STRING" id="273116.gene:9381158"/>
    <dbReference type="PaxDb" id="273116-14324596"/>
    <dbReference type="GeneID" id="1440893"/>
    <dbReference type="KEGG" id="tvo:TVG0368274"/>
    <dbReference type="eggNOG" id="arCOG01585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_4_1_2"/>
    <dbReference type="OrthoDB" id="33200at2157"/>
    <dbReference type="PhylomeDB" id="Q97BS4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013603">
        <property type="entry name" value="TRASH_TR_C_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08394">
        <property type="entry name" value="Arc_trans_TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="175" mass="20297" checksum="F6A1C18832E676AD" modified="2001-10-01" version="1">MHPEKRNLSNLESRVLRELIDDSRSSVEEISERTGIGRNTVSKIIKDLERNGIIKKYTIKTSYEESEKKIIALTPDDVHIPDDLIYYNFKLSNGKRILVLSKSALDYDFQYDQVWISDTVEEGKAILSNITLYCDYCGNQIHGDPISVKWKNRTYLVCCPNCEKDMLKRLEKDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BT0</accession>
    <name>Q97BT0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sec-independent protein translocase protein TatA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0364778</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BT0"/>
    <dbReference type="STRING" id="273116.gene:9381152"/>
    <dbReference type="PaxDb" id="273116-14324590"/>
    <dbReference type="KEGG" id="tvo:TVG0364778"/>
    <dbReference type="eggNOG" id="arCOG02694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_3_1_2"/>
    <dbReference type="PhylomeDB" id="Q97BT0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982:SF1">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11545" checksum="95D35D67CE9E82F2" modified="2001-10-01" version="1">MYDNMLDSAIEWLIVIVVILVLFGSAKKVPELARNVGRATGEFKRGQMEIEQELRKAMNSNNTAQTASQGVDYISVAKTMGIETEGKSIDELKAEIQEKLKNAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BW0</accession>
    <name>RL6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl6</name>
        <name type="ordered locus">TV0345</name>
        <name type="ORF">TVG0340711</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916599.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BW0"/>
    <dbReference type="SMR" id="Q97BW0"/>
    <dbReference type="STRING" id="273116.gene:9381122"/>
    <dbReference type="PaxDb" id="273116-14324560"/>
    <dbReference type="GeneID" id="1440857"/>
    <dbReference type="KEGG" id="tvo:TVG0340711"/>
    <dbReference type="eggNOG" id="arCOG04090">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_0_0_2"/>
    <dbReference type="OrthoDB" id="7144at2157"/>
    <dbReference type="PhylomeDB" id="Q97BW0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000008">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_A">
        <property type="entry name" value="Ribosomal_uL6_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019907">
        <property type="entry name" value="Ribosomal_uL6_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004037">
        <property type="entry name" value="PRK05518.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03653">
        <property type="entry name" value="uL6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000261000" description="Large ribosomal subunit protein uL6">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="178" mass="20025" checksum="BDC6CEE91806B332" modified="2001-10-01" version="1">MIKWEEASVIEIPKDVKVGLSGTMLSMTFGNKKLEKKFADNYVRLLVEDNKIKIVKSKNNSRERGIVGTWASEISNMVKGLKEGFQYEMKIDYSHFPMRVSVKGKTVVIENFFGERSPRTAEIVGETQVSVKGDRLFLNGPSKKDVGETAANIERATIIKGFDPRVFQDGIYLISKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BY2</accession>
    <name>Q97BY2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TVG0327166 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0327166</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916578.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BY2"/>
    <dbReference type="STRING" id="273116.gene:9381099"/>
    <dbReference type="PaxDb" id="273116-14324538"/>
    <dbReference type="GeneID" id="1440836"/>
    <dbReference type="KEGG" id="tvo:TVG0327166"/>
    <dbReference type="eggNOG" id="arCOG02937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_862228_0_0_2"/>
    <dbReference type="OrthoDB" id="275333at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048471">
        <property type="entry name" value="M3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048470">
        <property type="entry name" value="M3K_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040848">
        <property type="entry name" value="mev_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21433">
        <property type="entry name" value="M3K_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="4">
        <location>
            <begin position="10"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Mevalonate-3-kinase C-terminal" evidence="3">
        <location>
            <begin position="186"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="320" mass="35980" checksum="040636EBA131405E" modified="2001-10-01" version="1">MSNSSITSVAYPTIGVVLLGGIANEKTRTPLHTSAGIAYTDSCGSIRTESTIYGDSEMHIYFNGTESKDENRSVKSVLERYRNELQSFFGKKDVSYSSLNYGILSGSSDAGAASIGAILSFIDKKNDIHDIENDIRMISESAGRSLHGGLTITWSDGYSAYTERVLGPEHFNNYAIVGFSFDYPRNPSDTIHQNIIKSKRYKQRTIDADEHAHEIKEMARTDDIEGIFEKAEEDTEEYHSILREVGVLVIRENMQKLIEFIKILRKEFWNSYIVTGGSNVYVIVRRDDLERLIHIKNTFGSKPKILNVAGPAWIKKVESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BY7</accession>
    <name>Q97BY7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Stomatin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0324117</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916574.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BY7"/>
    <dbReference type="STRING" id="273116.gene:9381093"/>
    <dbReference type="PaxDb" id="273116-14324533"/>
    <dbReference type="GeneID" id="1440832"/>
    <dbReference type="KEGG" id="tvo:TVG0324117"/>
    <dbReference type="eggNOG" id="arCOG01915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_3_3_2"/>
    <dbReference type="OrthoDB" id="10752at2157"/>
    <dbReference type="PhylomeDB" id="Q97BY7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="6">
        <location>
            <begin position="21"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="274" mass="30346" checksum="A106EDD3532E5349" modified="2001-10-01" version="1">MTGLDILLIIIIIIALIILLSGIHVLKEWERAIVLTLGRYGGIRGPGIIFITPIVSRGIYVSTRIQPVQFKTEATFTKDNVPVNVDAIMYYQVIDPQKAVLNIENYSVGTNYAAQTTLREVIGKSMFDELLSEREKVGETAREIIDQKTEAWGVKVASVEIRDVIVPSQLQEAMSRQASAERERRSRVTLAQAEVEAAQKMVEASKQYVDNPVGLQLRWMQIIYEIGLEGKSSMIMVPSDVPVAGSAFSVMGMNEALKARGHVEDVQGQGSQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C12</accession>
    <name>Q97C12_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0303941 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0303941</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916548.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C12"/>
    <dbReference type="STRING" id="273116.gene:9381067"/>
    <dbReference type="PaxDb" id="273116-14324508"/>
    <dbReference type="GeneID" id="1440806"/>
    <dbReference type="KEGG" id="tvo:TVG0303941"/>
    <dbReference type="eggNOG" id="arCOG07412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114394_0_0_2"/>
    <dbReference type="OrthoDB" id="56235at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="208" mass="24507" checksum="219C81E7B38992C9" modified="2001-10-01" version="1">MIYDFGEDGYKITLFGGIKGLVRDGEELKRDLYEVRPEIIYIVLTQEQIDGLNNFLKDPFELSLSDYEIIYGLHLSAYGEVMTPPPIYIEAIKYANETGTTLVPLDVPEKEYSDYYSKNVGFLDLLRNSLRKKRIMKMEFGDKTPEDFVEKWDAVMHKVKGIERVDTFRFNYVRDNLKRNLEKDKGKSVFVITEYEFYKNLENFIKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="145" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C31</accession>
    <name>Q97C31_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="4">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="4">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">sucD</name>
        <name evidence="9" type="ORF">TVG0284189</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 7">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 7">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 6">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916531.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C31"/>
    <dbReference type="STRING" id="273116.gene:9381047"/>
    <dbReference type="PaxDb" id="273116-14324488"/>
    <dbReference type="GeneID" id="1440789"/>
    <dbReference type="KEGG" id="tvo:TVG0284189"/>
    <dbReference type="eggNOG" id="arCOG01339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_2"/>
    <dbReference type="OrthoDB" id="55711at2157"/>
    <dbReference type="PhylomeDB" id="Q97C31"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000006">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000277">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4 7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="8">
        <location>
            <begin position="3"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="4 5">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="285" mass="30368" checksum="4F713E93D487F14A" modified="2001-10-01" version="1">MVLIDKNTKVIVQGITGHQGSFHTGEMLKFGTNVVAGIAPGKGGTKFADKVPIVNSVAEAMQYEPDATMISVPAPFVKDAAYEAIDNGLKIVYILTEHVPYQDTMEIVKEAQLRNITVLGPNGPGITVPFESKIGIMPNHIFKEGDVAVASRSGTLTYEIVYAITKAGMGESTVIGLGGDRVVGLSFIDVLKMFENDPKTKKIVLVGEIGGNNEELAAEYIKKNVKKKVVAYIAGRSAPPGKRMGHAGAIIERGVGTAESKLKAFNSAGVKVADYPQDIPKLLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C62</accession>
    <name>Q97C62_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Formate hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">TVG0257496</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C62"/>
    <dbReference type="STRING" id="273116.gene:9381014"/>
    <dbReference type="PaxDb" id="273116-14324457"/>
    <dbReference type="KEGG" id="tvo:TVG0257496"/>
    <dbReference type="eggNOG" id="arCOG01492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_2_2_2"/>
    <dbReference type="PhylomeDB" id="Q97C62"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02792">
        <property type="entry name" value="MopB_CT_Formate-Dh-Na-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02753">
        <property type="entry name" value="MopB_Formate-Dh-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.90:FF:000001">
        <property type="entry name" value="Formate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000003">
        <property type="entry name" value="Formate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.228.10:FF:000002">
        <property type="entry name" value="Formate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000032">
        <property type="entry name" value="Formate dehydrogenase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000005">
        <property type="entry name" value="Periplasmic nitrate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.90">
        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041924">
        <property type="entry name" value="Formate_Dh-H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF14">
        <property type="entry name" value="FORMATE DEHYDROGENASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036643">
        <property type="entry name" value="FDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="13">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="16">
        <location>
            <begin position="81"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="14">
        <location>
            <begin position="141"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="14">
        <location>
            <begin position="184"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="15">
        <location>
            <begin position="262"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAB59385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="981" mass="109662" checksum="26ECE0BA0CD89006" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C64</accession>
    <name>Q97C64_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Citrate synthase</fullName>
            <ecNumber evidence="6">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0252956</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 8">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="6"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916495.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C64"/>
    <dbReference type="STRING" id="273116.gene:9381012"/>
    <dbReference type="PaxDb" id="273116-14324455"/>
    <dbReference type="GeneID" id="1440755"/>
    <dbReference type="KEGG" id="tvo:TVG0252956"/>
    <dbReference type="eggNOG" id="arCOG04237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025068_2_1_2"/>
    <dbReference type="OrthoDB" id="21302at2157"/>
    <dbReference type="PhylomeDB" id="Q97C64"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06118">
        <property type="entry name" value="citrate_synt_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011278">
        <property type="entry name" value="2-MeCitrate/Citrate_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054926">
        <property type="entry name" value="Cit_synThplmales"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024176">
        <property type="entry name" value="Citrate_synthase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01800">
        <property type="entry name" value="cit_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041157">
        <property type="entry name" value="Cit_synThplmales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010640">
        <property type="entry name" value="PRK14037.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871:SF1">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001369">
        <property type="entry name" value="Citrate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" evidence="7">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001369-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="386" mass="43001" checksum="3577BBD6BBCCE6AD" modified="2001-10-01" version="1">MPETTEEISKGLEDVNIKWTRLTTIDGNKGILRYGGYSVEDIINNGAQAEEIQYLFLYGELPNAQELKIFKENVQKGYNIPDFVINSIRQLPRESDAVAMQMAAVASMAASEIKFKWNKDTDRDVAAQMIGSMSAITANVYRHILGMPAERPKPSDSYAESFLKAAFGRNVTKEEVDAINTALILYTDHEVPASTTAGLVAVSTLSDIYSGITAALAALKGPLHGGAAEAAIAQFDEIKEPSNVEKWFNDNIINGKKRLMGFGHRVYKTYDPRAKIFKGIAENLSKNNAEVKKIYDIATKLEDLGVKQFGSKGIYPNTDYFSGIVYMSVGFPLRNNIYTALFALSRVTGWEAHFIEYVEEQQRLIRPRAVYVGPAERKFVKLPDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C77</accession>
    <name>Q97C77_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cell division protein SepF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0233939</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C77"/>
    <dbReference type="STRING" id="273116.gene:9380997"/>
    <dbReference type="PaxDb" id="273116-14324440"/>
    <dbReference type="KEGG" id="tvo:TVG0233939"/>
    <dbReference type="eggNOG" id="arCOG02263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131897_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q97C77"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="entry name" value="SepF-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012426">
        <property type="entry name" value="SepF_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019313">
        <property type="entry name" value="UCP019313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15863" checksum="7DC30EAA8722664D" modified="2001-10-01" version="1">MSSKAHHWKRLNIDIRSIIMLFKRKQDQKKTDAKVKYIDLVRFPPDESSEDHKIVKVAEVYSYEDLLETVKYPYSGNILIVDISGVSDEMASRRIMEKCATIGKEINGDVARVSKNMIMITPNGIKIDREKFRGSMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CC4</accession>
    <name>Q97CC4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0185867 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0185867</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916433.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CC4"/>
    <dbReference type="STRING" id="273116.gene:9380948"/>
    <dbReference type="PaxDb" id="273116-14324392"/>
    <dbReference type="GeneID" id="1441663"/>
    <dbReference type="KEGG" id="tvo:TVG0185867"/>
    <dbReference type="eggNOG" id="arCOG07355">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2893473_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="62" mass="7172" checksum="02FAC96A7B8AEEF5" modified="2001-10-01" version="1">MDQILDIFNENPDVFLTEEQIMSIVGTKDVDKKLKELVKEGKLKKIEVNKKSGRKIYYGISN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CE8</accession>
    <name>Y153_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0284 protein TV0153</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0153</name>
        <name type="ORF">TVG0162980</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0284 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CE8"/>
    <dbReference type="SMR" id="Q97CE8"/>
    <dbReference type="STRING" id="273116.gene:9380923"/>
    <dbReference type="PaxDb" id="273116-14324367"/>
    <dbReference type="KEGG" id="tvo:TVG0162980"/>
    <dbReference type="eggNOG" id="arCOG04272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053134_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CE8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01086">
        <property type="entry name" value="UPF0284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002805">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003372">
        <property type="entry name" value="PRK04447.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811:SF1">
        <property type="entry name" value="UPF0284 PROTEIN SLL1500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="Nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151064" description="UPF0284 protein TV0153">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01086"/>
        </source>
    </evidence>
    <sequence length="338" mass="36770" checksum="B0AC8AE50796E3DE" modified="2001-10-01" version="1">MEDIIFGKRSNVFDVNPENTVFVLIAGTSEVSTVPGITSAGATPELTRLTPAIDSEIIGEGKCLSSKDPPMTPEGIPTPAIVTKAILSLTNIRYIIVNGGFYAKPKTPFIETGLKPSNNPAVKTAIEDLDAAIHAGEHIGRLLDGVFENIILAESVPGGTTTAYAVLKAIGINAKTSSSMKEDPFSIKEKIAEMSFRRKLEKSPLGAVKEYGDNMMAVSLGISSSVKRSKIIFGGGTQMATVMYLDRLINGMRDRYISTTGWVYYHRTELFSDLGLSDYLAVSAMDFSGMKHPGLRYYQYGHVREGTGMGAAFLLARLMNFEAEAIYKSVDDFYEQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CI8</accession>
    <name>Q97CI8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR system endoribonuclease Csx1 CARF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0120325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916370.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CI8"/>
    <dbReference type="STRING" id="273116.gene:9380881"/>
    <dbReference type="PaxDb" id="273116-14324327"/>
    <dbReference type="GeneID" id="1441600"/>
    <dbReference type="KEGG" id="tvo:TVG0120325"/>
    <dbReference type="eggNOG" id="arCOG03433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_677271_0_0_2"/>
    <dbReference type="OrthoDB" id="102285at2157"/>
    <dbReference type="PhylomeDB" id="Q97CI8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10640">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013383">
        <property type="entry name" value="CRISPR-assoc_prot_DxTHG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052875">
        <property type="entry name" value="CRISPR_assoc_ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010171">
        <property type="entry name" value="CRISPR_Csx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053857">
        <property type="entry name" value="Csx1_CARF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01897">
        <property type="entry name" value="cas_MJ1666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02549">
        <property type="entry name" value="CRISPR_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169:SF1">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22230">
        <property type="entry name" value="Csx1_CARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160980">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR system endoribonuclease Csx1 CARF" evidence="1">
        <location>
            <begin position="4"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="406" mass="48217" checksum="8F3C8B06C887A72C" modified="2001-10-01" version="1">MKSIFISLWGNPERWNVTRYSFNSKTCESITSSRVIYENFKTDYCIIYIPNSIYPGIYKDDTYLSQYQDLINSKLCEFLNSKDTESAEFYKNSNKIIFPSKGSFTADNKNYIFDFDINYIYSYFYRSILNYLESVDKINKIIVDITHGINFLEFIFLESLMYALRVYALSNKIDVNIEYYNSDPYSNFAILNIYKIKSIEIEYKNVLSIISRDFINMYRHNKKDIKDYFKLNFNEDLSDYIKACSIMAQSVTVPYLMYLIINIYKKIYEIGSGGLEVHLNIEDNKIRQTFDNKINVNIETLAFLDTLIKINSSIKYDNGFKIPDIYTFTDNFFSRESYNIFIKNELRKIKSKKEHYDFDIGNFSRNFKAYAGLLDGLYVLKDNSIDFKTFHFQNQKIDLNWIVKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CK3</accession>
    <name>Q97CK3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="8">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG0102621</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="8">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="8"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916355.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CK3"/>
    <dbReference type="STRING" id="273116.gene:9380866"/>
    <dbReference type="PaxDb" id="273116-14324312"/>
    <dbReference type="GeneID" id="1441585"/>
    <dbReference type="KEGG" id="tvo:TVG0102621"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_1_2"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="PhylomeDB" id="Q97CK3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0274">FAD</keyword>
    <keyword evidence="2 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7 8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="10">
        <location>
            <begin position="2"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="9">
        <location>
            <begin position="325"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="436" mass="47229" checksum="D45C5F879224EB29" modified="2001-10-01" version="1">MYDAVVLGAGPGGYAAAIRLGQRKKKVAIIEKDKIGGECLNYGCIPSKAIIELANSINYLKEMPGVSINYNVDMKKWQEWKWSMINKLTGGVELLLKAYGVDIFRGTGYIQDKNHVKVNDKVLETDSLVIATGSKPVSINGINDVMYNREVLDLDHIPSSIVIIGGGYIGVEIGIALAKLGSKVTIVEMMPSILPGTDNELVRHVVRRMSQLGINVITGRKVLSTAKNGEVTVKLDDGQELRAEKVLMTVGRIPNTEGFGLENLKLAMDGRFIKTDSRKMTSVKGVYAIGDVSGQPMLAHKAYYEADIAADNICGIDSEVDYRAMPYVIYSDPEIAYTGVKGAKSTRFPVAANGRSLTMNENIGTFNIYYDEKGIVTGAGIAAPHASELISEISLAVESGLMAMDIGLTIHPHPTVSEGVKESAEEVYGKPLHFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CK5</accession>
    <name>Q97CK5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Potassium channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0100811</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916353.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CK5"/>
    <dbReference type="STRING" id="273116.gene:9380864"/>
    <dbReference type="PaxDb" id="273116-14324310"/>
    <dbReference type="DNASU" id="1441583"/>
    <dbReference type="GeneID" id="1441583"/>
    <dbReference type="KEGG" id="tvo:TVG0100811"/>
    <dbReference type="eggNOG" id="arCOG01958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_810434_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CK5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF11">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL KCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="4">
        <location>
            <begin position="111"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="3">
        <location>
            <begin position="212"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="342" mass="37906" checksum="64098EF7F093071B" modified="2001-10-01" version="1">MYVYSLKILGKTYKLSQNTFSFLTIFDGFFLTLGGYGLQEKTRAAWFIVTADAFLTFALLIISYARTYHIVFIGAIFDLYLILTLIKRRSEFVYPSKTIGRPEVGIAILTVLFTIAYGVGGSILSGEQFKPPIRSLGTALYYTGETVTTLGFGDILPVDLESRLFTISLAFLGVAIFFSAMTALITPTIERRVGGLVNRMEKRELMKLKDFVLVCGYSPLLSSYLLMLKQMGKVIVIVEKDPAIAAKLRDDGYIVLNQEADDQNLLSSFTMDDAYKIIIGPEDDAYSLIIAATLRQVAGEKAAGKTRVIVYNTKNVKKFSPFGYGVIDLPKIVSESLPPKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CL7</accession>
    <name>Q97CL7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0084357 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0084357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916341.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CL7"/>
    <dbReference type="STRING" id="273116.gene:9380850"/>
    <dbReference type="PaxDb" id="273116-14324298"/>
    <dbReference type="GeneID" id="1441571"/>
    <dbReference type="KEGG" id="tvo:TVG0084357"/>
    <dbReference type="eggNOG" id="arCOG07417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567265_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="170" mass="18236" checksum="11C06F64F638EA80" modified="2001-10-01" version="1">MPKPPDVGTTDALKKARKIATIAAVVIVVASFSVFYAMSLEDSVTVNVMYVNVYFYSGNSLISAYVIQYNHDTLSHDQKVTVPFEVNGGKDGITVNNLVSFTPGFSEVSLSTKSGLTITGAASPSATYGQAINIPAGETVTLYITFITPQHNYAGNFYANFTVSPYNKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CM9</accession>
    <name>Q97CM9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0074006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916329.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CM9"/>
    <dbReference type="SMR" id="Q97CM9"/>
    <dbReference type="STRING" id="273116.gene:9380838"/>
    <dbReference type="PaxDb" id="273116-14324286"/>
    <dbReference type="GeneID" id="1441559"/>
    <dbReference type="KEGG" id="tvo:TVG0074006"/>
    <dbReference type="eggNOG" id="arCOG05346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_579535_0_0_2"/>
    <dbReference type="OrthoDB" id="30676at2157"/>
    <dbReference type="PhylomeDB" id="Q97CM9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="466" mass="54041" checksum="65C5E0158575DF4A" modified="2001-10-01" version="1">MFYYERVNQFDTIAVSVDSVDRRRIKGTIDLDGLKFIMYFDYTQDVDIDYDLAGMIMTMPVINYAYFAKRIKLNFPVSLSFLKLVKEFIRINNIETFINSIVRRRYEFFLKEYLPSDEEITQENAEGRTEVLADISDGDKKKLEVDDRKVIVLSSGGKESLLTFGILKEIGAVVYPVFFNESGAHWKTAKVAYDYFSSKYENVLRVWSNVDRFYSFVNRHLKILDQDEIRRKADSYPVQMFIFPVYIFSVLPYVIKYGIGNIALGDELDDPIYEEPYHGIKHYYGVYDQTNDFNLTLTSFLREYGIHCKVFSAVYPIFGTLVEKILVQRYNDLFRVQRSCHLCHYEGGDVKPCGTCSKCLGVLLFMLNAEADPTEAGYSIEHVKQLEDNVNSASMRLDPDELEYLKAKVFEGRRINSHIEGIHQLPGENSAFSLVPERFRDGVRSIVSKYSTGFYKFVGSEWVKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CN8</accession>
    <name>Q97CN8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0064976 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0064976</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_052268453.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CN8"/>
    <dbReference type="STRING" id="273116.gene:9380829"/>
    <dbReference type="PaxDb" id="273116-14324277"/>
    <dbReference type="GeneID" id="71201305"/>
    <dbReference type="KEGG" id="tvo:TVG0064976"/>
    <dbReference type="eggNOG" id="arCOG02640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2476211_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="87" mass="9889" checksum="585410B2BE715764" modified="2001-10-01" version="1">MVNSHFLKRIISVGEKEILARLTDYADLSYKATDILIFMMKDCSSLVERNEEVKEIEKVGDELNISIKQDITKGNKPKPYGQLDTIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-15" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CQ3</accession>
    <name>AATE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpE</name>
        <name type="ordered locus">TV0048</name>
        <name type="ORF">TVG0050546</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59190" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59190.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916305.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CQ3"/>
    <dbReference type="SMR" id="Q97CQ3"/>
    <dbReference type="STRING" id="273116.gene:9380813"/>
    <dbReference type="PaxDb" id="273116-14324262"/>
    <dbReference type="GeneID" id="1441535"/>
    <dbReference type="KEGG" id="tvo:TVG0050546"/>
    <dbReference type="eggNOG" id="arCOG00869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1458252_0_0_2"/>
    <dbReference type="OrthoDB" id="57284at2157"/>
    <dbReference type="PhylomeDB" id="Q97CQ3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002264">
        <property type="entry name" value="PRK01194.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000117328" description="A-type ATP synthase subunit E">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="185" mass="21103" checksum="ED146DBF00B98888" modified="2002-05-15" version="2">MSLEQVIKEIEQSQEIKKKEILENTKNILDKMEAEKKAKIDEIRALYQEKMRAESSRITASIIDKANIEARSILRNKIEEILEGYVSAAQEILRNIRNMPEYPALLNKMIEVAKKYLGQDCIIKVDSKDKAVAPSSGITYSNIDPYGGILASSRDGKVELDLRVSSIMEEVLEKIKVKIYTRLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CQ7</accession>
    <name>Q97CQ7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0046979 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0046979</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916302.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CQ7"/>
    <dbReference type="SMR" id="Q97CQ7"/>
    <dbReference type="PaxDb" id="273116-14324258"/>
    <dbReference type="DNASU" id="1441531"/>
    <dbReference type="GeneID" id="1441531"/>
    <dbReference type="KEGG" id="tvo:TVG0046979"/>
    <dbReference type="eggNOG" id="arCOG02738">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167327_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="118" mass="13320" checksum="5CC57D7A44618BF6" modified="2001-10-01" version="1">MKVAAVVDENNTLQPLDMGSSIILFDDEKKEMKEYENPGFANPRGGKEIAMSIILRLKPDAVLVKEGFLCPGSYGMSRGRIKYIPAESEDLTENIKNISELKEKAQSELSFEFFRENE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-23" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CS3</accession>
    <name>PYRF_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="1">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">OMP decarboxylase</fullName>
            <shortName evidence="1">OMPDCase</shortName>
            <shortName evidence="1">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrF</name>
        <name type="ordered locus">TV0028</name>
        <name type="ORF">TVG0029151</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>orotidine 5'-phosphate + H(+) = UMP + CO2</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the OMP decarboxylase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_156769028.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CS3"/>
    <dbReference type="SMR" id="Q97CS3"/>
    <dbReference type="STRING" id="273116.gene:9380793"/>
    <dbReference type="PaxDb" id="273116-14324242"/>
    <dbReference type="GeneID" id="1441514"/>
    <dbReference type="KEGG" id="tvo:TVG0029151"/>
    <dbReference type="eggNOG" id="arCOG00081">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067069_2_0_2"/>
    <dbReference type="OrthoDB" id="94124at2157"/>
    <dbReference type="PhylomeDB" id="Q97CS3"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01200_A">
        <property type="entry name" value="OMPdecase_type1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047595">
        <property type="entry name" value="OMPdecase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010386">
        <property type="entry name" value="PRK13813.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000134621" description="Orotidine 5'-phosphate decarboxylase">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01200"/>
        </source>
    </evidence>
    <sequence length="219" mass="24069" checksum="521C585E62F4B267" modified="2001-10-01" version="1">MDKRIIVALDVKEKKKAIDIAESLSDIVFAFKINWPLVLYSSPEVIGEISQYGKVICDFKVADIPYTNSLITERVRDLGAWGIISHSFLGEESLKSVVNAAKGMHVFSVVAMSHPGSDMINSNAMQLMKLSIECGVYGFVAPANKIDDLRMIRSATDRVIISPGIGAQGGDPYTAVLNGSDYLIVGRSVYESDKPELEVSKLQQTAERAIEDRERLKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CU9</accession>
    <name>Q97CU9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Formate ferredoxin oxidoreductase [FMOR] alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0003442</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916259.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CU9"/>
    <dbReference type="STRING" id="273116.gene:9380767"/>
    <dbReference type="PaxDb" id="273116-14324216"/>
    <dbReference type="GeneID" id="1441487"/>
    <dbReference type="KEGG" id="tvo:TVG0003442"/>
    <dbReference type="eggNOG" id="arCOG01491">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_4_0_2"/>
    <dbReference type="OrthoDB" id="23466at2157"/>
    <dbReference type="PhylomeDB" id="Q97CU9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02790">
        <property type="entry name" value="MopB_CT_Formate-Dh_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02753">
        <property type="entry name" value="MopB_Formate-Dh-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.228.10:FF:000002">
        <property type="entry name" value="Formate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.90">
        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041925">
        <property type="entry name" value="CT_Formate-Dh_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041924">
        <property type="entry name" value="Formate_Dh-H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF14">
        <property type="entry name" value="FORMATE DEHYDROGENASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="669" mass="74471" checksum="C10414A526915F0E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>P58299</accession>
    <name>RL10E_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L10e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl10e</name>
        <name type="ordered locus">TV0539</name>
        <name type="ORF">TVG0528148</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916797.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58299"/>
    <dbReference type="SMR" id="P58299"/>
    <dbReference type="STRING" id="273116.gene:9381324"/>
    <dbReference type="PaxDb" id="273116-14324754"/>
    <dbReference type="GeneID" id="1441055"/>
    <dbReference type="KEGG" id="tvo:TVG0528148"/>
    <dbReference type="eggNOG" id="arCOG04113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084051_0_2_2"/>
    <dbReference type="OrthoDB" id="30538at2157"/>
    <dbReference type="PhylomeDB" id="P58299"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00448">
        <property type="entry name" value="Ribosomal_uL16_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022981">
        <property type="entry name" value="Ribosomal_uL16_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000147151" description="Large ribosomal subunit protein uL16">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="176" mass="19377" checksum="E284F9F14A187B46" modified="2001-09-26" version="1">MVTKPARMYTRITGPAYTRKEFMGGVPYPKITTFVQGNQKRDFPIEMQLIAMESCQVRHTALEAARVSVNRRMTEAAGLDNFYLKVVPYPHHVLREHKMATGAGADRISSGMRAAFGRPVGTAARVYQNDVIMIGRTDEAHAHELKIALKKAAIKLPTPCKVVITKGKEIAGAIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q978E5</accession>
    <name>Q978E5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1523868 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1523868</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978E5"/>
    <dbReference type="STRING" id="273116.gene:9382286"/>
    <dbReference type="PaxDb" id="273116-14325711"/>
    <dbReference type="KEGG" id="tvo:TVG1523868"/>
    <dbReference type="eggNOG" id="arCOG05337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712987_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="80" mass="8772" checksum="87C771D80609C188" modified="2001-10-01" version="1">MLYATSDIMADELSDNAKKIYDAMKKLGATSEEKLKTADDIMKAAALGKSIVNAALQELQKKGYVKRVARQKSAGYFVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q978G3</accession>
    <name>Q978G3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1503014</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917683.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978G3"/>
    <dbReference type="PaxDb" id="273116-14325691"/>
    <dbReference type="GeneID" id="1442141"/>
    <dbReference type="KEGG" id="tvo:TVG1503014"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="PhylomeDB" id="Q978G3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17321">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="11"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="459" mass="49844" checksum="BD258D580AC22628" modified="2001-10-01" version="1">MIQNYNRKYFVLSITTLGSLMAAIDSTIVFLALPSMGSYFSANVSSLSWIVVSYILSSTVFMIPSSDIMRKVGKKALYLAGFLIFGFSSLIIAVSPTIIIAIIFRFVEGIGAGFLSAVGIPILLDAFPPQERGKAVGINSISWAIGTLVGPVLGGYLVLYDWRYVFLINVPIGVVAFALGLIRIPKYQKERAKRFNPIPLLGLSAFLIPLTIGISFINFYYLAAAAIMFPIFIITQRRSSIIPSELLHNKNYVRISIASTLQALSFFGVLYALSMYLQISLHYSSLTAALFLFTYPLASMISNPLSGMLYDRTGRGPLLLGAGLVMQAFGIFMAAYTMKLIPVYLFIAGFGGSMFWAPSTTMVVDAAGPKFRGIANSSLFTLRNMSLIIGISIFPLFVSAFSSVKSSSIIITASGINLYSATSFYLMMVASLSVLALIPLVSYARTKKTAIAPSNENVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q978G4</accession>
    <name>Q978G4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG1502957 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1502957</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978G4"/>
    <dbReference type="STRING" id="273116.gene:9382263"/>
    <dbReference type="PaxDb" id="273116-14325690"/>
    <dbReference type="KEGG" id="tvo:TVG1502957"/>
    <dbReference type="eggNOG" id="arCOG01533">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_319962_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.76.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="2">
        <location>
            <begin position="386"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="928" mass="103143" checksum="6FB59B34A1B2B1BF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q978J0</accession>
    <name>Y1425_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Putative ankyrin repeat protein TV1425</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV1425</name>
        <name type="ORF">TVG1472127</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917657.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="2RFM">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.65 A"/>
        <property type="chains" value="A/B=1-189"/>
    </dbReference>
    <dbReference type="PDBsum" id="2RFM"/>
    <dbReference type="AlphaFoldDB" id="Q978J0"/>
    <dbReference type="SMR" id="Q978J0"/>
    <dbReference type="STRING" id="273116.gene:9382234"/>
    <dbReference type="PaxDb" id="273116-14325664"/>
    <dbReference type="GeneID" id="1442115"/>
    <dbReference type="KEGG" id="tvo:TVG1472127"/>
    <dbReference type="eggNOG" id="arCOG04004">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_18_1_2"/>
    <dbReference type="OrthoDB" id="56560at2157"/>
    <dbReference type="PhylomeDB" id="Q978J0"/>
    <dbReference type="EvolutionaryTrace" id="Q978J0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0040">ANK repeat</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000067234" description="Putative ankyrin repeat protein TV1425">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK 1">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK 2">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK 3">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK 4">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="8"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="21"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="45"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="48"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="68"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="101"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="111"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="134"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="144"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="167"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="2RFM"/>
        </source>
    </evidence>
    <sequence length="189" mass="21312" checksum="EE76005939D27A40" modified="2001-10-01" version="1">MDKNGEIVEKIKDEKSINQNLDFLRNYRDSYNRTPLMVACMLGMENAIDKLVENFDKLEDKDIEGSTALIWAVKNNRLGIAEKLLSKGSNVNTKDFSGKTPLMWSIIFGYSEMSYFLLEHGANVNDRNLEGETPLIVASKYGRSEIVKKLLELGADISARDLTGLTAEASARIFGRQEVIKIFTEVRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q978J9</accession>
    <name>Q978J9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe-S cluster assembly protein SufB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1463374</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978J9"/>
    <dbReference type="STRING" id="273116.gene:9382225"/>
    <dbReference type="PaxDb" id="273116-14325655"/>
    <dbReference type="KEGG" id="tvo:TVG1463374"/>
    <dbReference type="eggNOG" id="arCOG01715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_0_1_2"/>
    <dbReference type="PhylomeDB" id="Q978J9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="3">
        <location>
            <begin position="57"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="2">
        <location>
            <begin position="216"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="53775" checksum="4659C78AAE531FCC" modified="2001-10-01" version="1">MESTYVNKDEEIDRLISDLKNGKALDYGFHDNIKPVYSTGKGLNSKVVEEISEIKKEPDWMRRIRLHALEIFNSKPVPTWGPDLSSIDWDNMTYYSSPDDVKATNWDEVPKEIKDTFEKLGVPEMERKYLAGSIAQYDSEGVYNSLKKEWEEKGVVFMDLDSALQRYPDLVKEYFAKAVPPSDNKFAALNVAVWSGGAFLYVPRGVHIDMPLQTYFRINGEATGQFEHTIVVADEGSSVHYLEGCTAPVYSKDSLHAAIVEYYVMKNAHGRYTSVQNWSNSVYNMPTKRAWVEENGNMEWVGGSLGSKVTMIYPSSYLRGRNATTSNLNIALASPGTVKDTGAKAIHMAPNTSSKIIAKSVSLGDGKSIYRGLVRINKDALNAKSYVQCDALLINDESVSYTMPHDEIYEPTANFTHEATVGKIGTEELTYLRSRGLSEDEASSLIVLGFLDDVMKEIPMEFAVEMNRLVKLEMSKMGAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q978L0</accession>
    <name>Q978L0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic dehypoxanthine futalosine synthase</fullName>
            <shortName evidence="6">Cyclic DHFL synthase</shortName>
            <ecNumber evidence="6">1.21.98.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dehypoxanthine futalosine cyclase</fullName>
            <shortName evidence="6">DHFL cyclase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Menaquinone biosynthetic enzyme MqnC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mqnC</name>
        <name evidence="10" type="ORF">TVG1452246</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Radical SAM enzyme that catalyzes the cyclization of dehypoxanthine futalosine (DHFL) into cyclic dehypoxanthine futalosine (CDHFL), a step in the biosynthesis of menaquinone (MK, vitamin K2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dehypoxanthine futalosine + S-adenosyl-L-methionine = cyclic dehypoxanthinylfutalosinate + 5'-deoxyadenosine + L-methionine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33083"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58864"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64270"/>
            <dbReference type="EC" id="1.21.98.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6 7">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. MqnC family.</text>
    </comment>
    <dbReference type="EC" id="1.21.98.1" evidence="6"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917637.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978L0"/>
    <dbReference type="STRING" id="273116.gene:9382214"/>
    <dbReference type="PaxDb" id="273116-14325644"/>
    <dbReference type="GeneID" id="1442095"/>
    <dbReference type="KEGG" id="tvo:TVG1452246"/>
    <dbReference type="eggNOG" id="arCOG00656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040406_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q978L0"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046992">
        <property type="term" value="F:oxidoreductase activity, acting on X-H and Y-H to form an X-Y bond"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00992">
        <property type="entry name" value="MqnC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022431">
        <property type="entry name" value="Cyclic_DHFL_synthase_mqnC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00423">
        <property type="entry name" value="CofH family radical SAM protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03699">
        <property type="entry name" value="menaquin_MqnC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="FO SYNTHASE (COFH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076:SF1">
        <property type="entry name" value="LIPOYL SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004762">
        <property type="entry name" value="CHP00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00342">
        <property type="entry name" value="cyclic_dehypoxanthine_futalosi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01064">
        <property type="entry name" value="F420__menaquinone_cofactor_bio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01389">
        <property type="entry name" value="menaquinone_synthsis_involved"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="53"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>(3R)-3-methyl-D-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:64642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>(3R)-3-methyl-D-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:64642"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="366" mass="41850" checksum="CACDF31AE82C528A" modified="2001-10-01" version="1">MIKTGELKYIEEKIERREDLDEDDIVYMYDEADVNDLGKLARSITDKISGKRVSFVSNIILNYTNICNVRCKFCAFYRKSGEYDAYTMTPDGVIDEIRKYYPYGIRQILIQGGVNPNLDLDYYLTVFRKIRENFTEAGINGLSAAEINFISKKAHLTYEETILKLKEAGLETIPGAGAEILDEDVRVKLGRPRGSGRQWLEIMETAHRLGVKTTATMVYGHIESSMDKARHLISIRNLQKKYHGFLSFTPWNMEPDNTELQKEGILKYRAGAEDVIRNIAISRIVFNYDLPIIQSSWLTNGVEMGQLAILYGANDWGGTIYDEKVIPATGKRVGNLRKEIIIRSIKDLGMVPVERDNLYREIAVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q978P9</accession>
    <name>Q978P9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1413828</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978P9"/>
    <dbReference type="STRING" id="273116.gene:9382174"/>
    <dbReference type="PaxDb" id="273116-14325605"/>
    <dbReference type="KEGG" id="tvo:TVG1413828"/>
    <dbReference type="eggNOG" id="arCOG02685">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_2"/>
    <dbReference type="PhylomeDB" id="Q978P9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="42"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="500" mass="54760" checksum="72DFF9EE513525EB" modified="2001-10-01" version="1">MDIFHIFSDKRLNTVYLSKNMEYHEVIEKLDFSKVTRFYWLLTLLATIGGFLFGYDTSNIGTALVFIPYPVQGFIEGYLVAGASLGAAVGALIAAGLTDRYGRKYLLIADAAIYAAGAILSAFTIDLAMLLLSRTLIGLAVGADSGIATAYISEYAPKDRRGSLGILQQWMITIGILGAYIVGMATLYAAPGLASVLDWRIMLGVAAIPALIGLGFRFMMPESPRWLMINGKYKEASEAFKKLGLNLSVDELKEVKFRQRAKITNGVKRAFLVVALFFVFQQITGINIPFYYGPIVIEKLHLFGSATQSLVFTQIYAVAAATILAVINVAATYIGFRYIDTVGRRKLALLGYSGMTFFGLLGGLLLYLKFDVGLLIAFAGFIIFFAFGVGGTGWMIQGEYFPTNMRGLYASLGAFVDWIANFAIIEIFPVLEALITIKNVMFVFGILSLIAVLIFYYIMPETKELSVEQISDLFENNGLSSLRKASMSYNKSSSGSSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q978R0</accession>
    <name>Q978R0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transport system permease protein P1P2A1A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1400273</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917589.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978R0"/>
    <dbReference type="STRING" id="273116.gene:9382163"/>
    <dbReference type="PaxDb" id="273116-14325594"/>
    <dbReference type="GeneID" id="1441471"/>
    <dbReference type="KEGG" id="tvo:TVG1400273"/>
    <dbReference type="eggNOG" id="arCOG00751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_3_2"/>
    <dbReference type="PhylomeDB" id="Q978R0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163:SF6">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="103"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="363" mass="39835" checksum="72515B2535E432DD" modified="2001-10-01" version="1">MELRFFIIRRLLLLIPTLIGLTLLTFILVRALPTSLLLSQYVNPHSTLPRSVQIAHAKAILGLDYPVPIQYFFYLAALIHGNWGFMVSNFYTGPVLTGIEEFFPNTLQLTIFAVILSVLISIPLGTYIGSKPNSLADHAGRIFSLAVYAMPAFVLALLFQIVFGRGAITGNPLGVFPISGTFNYALVPFPVPSWLTTPGTSALVSHPTHLIIFDALIHHNYAIAYSGLLHIVLPVLTLTLGILAGIVRFLRAGMMDNMRQEYVKTARAKGVPEKVIIRRHIRKNALIPTVTVLGLLFASLLGGVVLIEDVFDYPGMGLLGINAITSYSIYGVMGVTLVFGLVLVFANLIVDVVYAFLDPRIRY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q978S6</accession>
    <name>AROB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate synthase</fullName>
            <shortName evidence="1">DHQS</shortName>
            <ecNumber evidence="1">4.2.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroB</name>
        <name type="ordered locus">TV1339</name>
        <name type="ORF">TVG1384310</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit. Can use either Co(2+) or Zn(2+).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917574.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978S6"/>
    <dbReference type="SMR" id="Q978S6"/>
    <dbReference type="STRING" id="273116.gene:9382147"/>
    <dbReference type="PaxDb" id="273116-14325578"/>
    <dbReference type="GeneID" id="1441456"/>
    <dbReference type="KEGG" id="tvo:TVG1384310"/>
    <dbReference type="eggNOG" id="arCOG00983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001201_0_1_2"/>
    <dbReference type="OrthoDB" id="21407at2157"/>
    <dbReference type="PhylomeDB" id="Q978S6"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08195">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00110">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050071">
        <property type="entry name" value="Dehydroquinate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030963">
        <property type="entry name" value="DHQ_synth_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43622">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43622:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24621">
        <property type="entry name" value="DHQS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001455">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000140825" description="3-dehydroquinate synthase">
        <location>
            <begin position="1"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="70"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="128"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00110"/>
        </source>
    </evidence>
    <sequence length="360" mass="39616" checksum="B46B1E00FC03E57E" modified="2001-10-01" version="1">MDTQRFVITMNGDNISFIVGDNAINHLSEEAGKYDSIVIMISKTVEEMYANHIPDVGSFGNSVVKISLNDGESLKSLRNYQKIVKVLLERKVDRRSLLVYIGGGTVGDLAGFVASTYKRGVMMIAVPTTLLAQVDSSIGGKNGLDFSDVKNVIGTFYNPYMVIDDTVFLKNNSFIIREGMSEVIKYAIISGGDMYDTLNRCSIDNFDACATNIIKLSVKIKSEIVNRDFYDRTGIRSVLNLGHTIAHGIEGATKGSISHGKAVATGMLVEAHIGEKYGNTNHEVIEAIRDLMKRYGIEELNLKEIGPGNILRYISNDKKIMEGYINMPVPSEIGKVITMKATERMISDGINTFIKENDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q978X1</accession>
    <name>Q978X1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Saccharopine dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1335287</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917529.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978X1"/>
    <dbReference type="STRING" id="273116.gene:9382101"/>
    <dbReference type="PaxDb" id="273116-14325533"/>
    <dbReference type="DNASU" id="1441411"/>
    <dbReference type="GeneID" id="1441411"/>
    <dbReference type="KEGG" id="tvo:TVG1335287"/>
    <dbReference type="eggNOG" id="arCOG00243">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032858_3_0_2"/>
    <dbReference type="OrthoDB" id="27588at2157"/>
    <dbReference type="PhylomeDB" id="Q978X1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="2">
        <location>
            <begin position="4"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="120"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="41119" checksum="4DD390BF27D3ABB5" modified="2001-10-01" version="1">MKGIVFGSGQIGSSVALNLVDGMDVTVADRDPTNLRKIQDNIGSRVSVIQVDALRDDIKHIISDYDIVVSALPGSVGFQFAKSIAPFGVRMIDISYYEDDVFLLDDVAKKSSSVIVPDIGFAPGISNVLVGHFSYELEDVKDVHIYVGGIPEKRIGGLDYVITWSVEGLLDEYTRPVHIVQNGSITQVEPLSGLEKINIQKYTDLEAFYTDGLRTLGKTIRASGSMWEKTVRYSGHAEKIRLLKDLGFFSRKKVKVGNSEIAPFDFTAEIFRNNLSMSGVKDVSLMYVKVTGTRNGDIVKHEASMVAPYDEKRKRSSMANVTSVPASATADFLSKNEIGKYGVVAPEILGKDENFYKQFINYLGQYGIIISED</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q978X3</accession>
    <name>Q978X3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase</fullName>
            <ecNumber evidence="2">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1331499</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917526.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978X3"/>
    <dbReference type="STRING" id="273116.gene:9382098"/>
    <dbReference type="PaxDb" id="273116-14325530"/>
    <dbReference type="GeneID" id="1441408"/>
    <dbReference type="KEGG" id="tvo:TVG1331499"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="OrthoDB" id="371752at2157"/>
    <dbReference type="PhylomeDB" id="Q978X3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="8">
        <location>
            <begin position="28"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="91"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="506"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="619" mass="68964" checksum="46C050C1651A49F4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Y6</accession>
    <name>Q978Y6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trehalose 6-phosphate phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1319820</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Removes the phosphate from trehalose 6-phosphate to produce free trehalose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="EC" id="3.1.3.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the trehalose phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.12" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917513.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Y6"/>
    <dbReference type="STRING" id="273116.gene:9382085"/>
    <dbReference type="PaxDb" id="273116-14325517"/>
    <dbReference type="DNASU" id="1441395"/>
    <dbReference type="GeneID" id="1441395"/>
    <dbReference type="KEGG" id="tvo:TVG1319820"/>
    <dbReference type="eggNOG" id="arCOG01216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037265_2_1_2"/>
    <dbReference type="OrthoDB" id="70105at2157"/>
    <dbReference type="PhylomeDB" id="Q978Y6"/>
    <dbReference type="UniPathway" id="UPA00299"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044651">
        <property type="entry name" value="OTSB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768">
        <property type="entry name" value="TREHALOSE 6-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768:SF3">
        <property type="entry name" value="TREHALOSE 6-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="246" mass="27907" checksum="B72F29913A5065B0" modified="2001-10-01" version="1">MIDGLLITELNKLTPGSSYIFLDYDGTLVPIIMNPEQSLADPELIEMLTELRSKYKLFIVSGRSPEEIKRFIPLDLNLICYHGACSIVNGKTYYYNNAQSFLGIFNEIYQSSLNLVNEFPGLRIYKKNLAVLYHFGLMKPQDVPALERVIVEISQKEHVDVYKGKMIFELRVPGVNKGIAIKNVRDEETALIAGDDATDECSFKYNEDAITIKVGPGKTSAHFRVNDYSEMREVLKLLLNNAEKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Y7</accession>
    <name>Q978Y7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alpha, alpha-trehalose-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1319114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Y7"/>
    <dbReference type="STRING" id="273116.gene:9382084"/>
    <dbReference type="CAZy" id="GT20">
        <property type="family name" value="Glycosyltransferase Family 20"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325516"/>
    <dbReference type="KEGG" id="tvo:TVG1319114"/>
    <dbReference type="eggNOG" id="arCOG02831">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_7_1_2"/>
    <dbReference type="PhylomeDB" id="Q978Y7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="447" mass="51999" checksum="AF8CB50F60B89033" modified="2001-10-01" version="1">MMRYVIASSRCPFSHDVVMGRELVKENVGGVATALKRAISKHGGTWICWGDGKADNRYVNEDVGTYKIKRIILTPQEKRGYYDMYSNRTLWPLFHYFRERVQYTDNGYETYRKVNEKFAEEILNNTSQEDIVWVHDYQLSLVPGILRQKGLKNRIIFTWHIPWVSREMFETLTESDEIKESVGSADFITFHTDLYRKNFESLFENRKHGKSIAVPLGIDYRYFEKAKSSQIKSYALKDKIMLFSIDRLDYTKGLTNRVLSIERLIRNHPETSGKFVYVMIVTPSRTSVSDYVSMKRELEMNIGRVNGEFGTISWMPILYMYRKISDHSLVSYYKSADIALITPLIDGLNLVSKEFIASTEKGILVLSKFAGASNCLRGALIVNPNSPDEVADAIYKAMNMERKEADERLLKMKAIVKARDTDWWIKRIYRLAIAKEHDRRVTNNRIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q979A1</accession>
    <name>DNLI_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polydeoxyribonucleotide synthase [ATP]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lig</name>
        <name type="ordered locus">TV1261</name>
        <name type="ORF">TVG1298537</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917495.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979A1"/>
    <dbReference type="SMR" id="Q979A1"/>
    <dbReference type="STRING" id="273116.gene:9382066"/>
    <dbReference type="PaxDb" id="273116-14325499"/>
    <dbReference type="GeneID" id="1441377"/>
    <dbReference type="KEGG" id="tvo:TVG1298537"/>
    <dbReference type="eggNOG" id="arCOG01347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005138_6_0_2"/>
    <dbReference type="OrthoDB" id="31274at2157"/>
    <dbReference type="PhylomeDB" id="Q979A1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000059622" description="DNA ligase">
        <location>
            <begin position="1"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="1">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <position position="255"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00407"/>
        </source>
    </evidence>
    <sequence length="588" mass="66218" checksum="7FC5FC2549F3CD3D" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q979A2</accession>
    <name>Q979A2_THEVO</name>
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            <fullName evidence="3">Single-stranded DNA binding protein Ssb-like OB fold domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="4" type="ORF">TVG1298535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
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            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmata</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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    <dbReference type="PaxDb" id="273116-14325498"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK06461.1-3"/>
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        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="SOSS COMPLEX SUBUNIT B HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
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        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="17"/>
            <end position="97"/>
        </location>
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            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="145" mass="16269" checksum="3447C7BB0E8E8B2E" modified="2001-10-01" version="1">MEDITKIKDLTPSSRRVNVLGKVLSVGEPKTIQTKFGDQRSVTEVTIGDDTGKVILSLWGDQAAQAKTGDTLMIGNGYVSLVRGHIRLNVGKYGSLTVSDEEVDEVNESYDASEKEYENVYRSNPHRGNFRRGNYRNNNRDEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q979A4</accession>
    <name>Q979A4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1296716 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1296716</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917492.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979A4"/>
    <dbReference type="PaxDb" id="273116-14325496"/>
    <dbReference type="GeneID" id="1441374"/>
    <dbReference type="KEGG" id="tvo:TVG1296716"/>
    <dbReference type="eggNOG" id="arCOG06972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444719_0_0_2"/>
    <dbReference type="OrthoDB" id="56644at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="201" mass="23496" checksum="DB55D44E8840924F" modified="2001-10-01" version="1">MSLASIDRYDYYLKIILNSSVRDYPYIVEEYQNDAEISESEAESLDRIVMKYMGIYRKSDVKIVNTMTGQEETAPLTIIDDFGGNAVYVGEQTLFGILWKIYQPPAYIERPIVNKWYYNEKIASILNEILYDAVPGKVKNYFDTPPWIIGTRYGVQSRYMPLYEIIIEAINRNLIPGWYYLARSGLSQEFIEAIRNRSFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q979A9</accession>
    <name>Q979A9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1290876 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1290876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917487.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979A9"/>
    <dbReference type="STRING" id="273116.gene:9382058"/>
    <dbReference type="PaxDb" id="273116-14325491"/>
    <dbReference type="GeneID" id="1441369"/>
    <dbReference type="KEGG" id="tvo:TVG1290876"/>
    <dbReference type="eggNOG" id="arCOG06008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_942587_0_0_2"/>
    <dbReference type="OrthoDB" id="56710at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="324" mass="35741" checksum="3A43C9B357DFD2CF" modified="2001-10-01" version="1">MIINLWNPSYEGLYAAIVISYLLGIVHGITPDEHTWPITFSYAVGNYSTKGGAKAGLVFSAGFTVERALMSEIAALALASFMFYTYFNSIVYVIVGAVMALSGFYIANKLKYPHIHIIEESLYRIFGIHKHNKEREKLELEHMENPVMVNEDGVYRPVPTKLAFVHGLIAGFGFGAFALIIYFVIAPNMPIYLGFLPGLMFGLGTMTMQVLAGTLFGLWLSKKKDLTVNGIAFVARGISHFVLSYGGITFVIAGIVTLIFPQLWNYQIITGIKIHNLDALGLPFFLVVLSVLVIGYLGYKINLNKAVKMEMTNSANGSKESTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q979C0</accession>
    <name>Q979C0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histidine triad protein [HIT]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1280493</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979C0"/>
    <dbReference type="STRING" id="273116.gene:9382046"/>
    <dbReference type="PaxDb" id="273116-14325479"/>
    <dbReference type="KEGG" id="tvo:TVG1280493"/>
    <dbReference type="eggNOG" id="arCOG00419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_3_3_2"/>
    <dbReference type="PhylomeDB" id="Q979C0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648:SF1">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="HIT FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="13"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="105"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="157" mass="18078" checksum="DF9EF0B7FE7BC752" modified="2001-10-01" version="1">MKLPVTMYEPSCVFCTEIIQKRNAAVVAENEYTIAFMDKAPVEPGHVLVIPKRHFINIFDIDDFYYIEVQKMVKRVSKAVLEALSADALNVGQNNGRCANQIVMHYHVHVIPRWCDRPFKWGRIEASFEELQNTAKLISETYDRLFSVKGKALSNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q979C7</accession>
    <name>Q979C7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG1270428 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1270428</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917469.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979C7"/>
    <dbReference type="STRING" id="273116.gene:9382039"/>
    <dbReference type="PaxDb" id="273116-14325472"/>
    <dbReference type="GeneID" id="1441351"/>
    <dbReference type="KEGG" id="tvo:TVG1270428"/>
    <dbReference type="eggNOG" id="arCOG05366">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080184_0_0_2"/>
    <dbReference type="OrthoDB" id="56286at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="CARDB" evidence="2">
        <location>
            <begin position="133"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="259" mass="28585" checksum="1BD220650C8E1271" modified="2001-10-01" version="1">MKSKYIVYSVALIFALLMVLSSVGSNAATAPFYTSSIKYPSDVGAGQSFNVTIVSTANFANYNFTVYFAGTNLTGISPTSTIHHYSANNPMYTFSMVAPSVPQTLYLYVVTSAKFDGIQYNYTQTISIDVVSPVYFHVELKNPTSYPIYNITATFYVDGIPVAAKTVPKIMPGSTVEVNVTYVNPNFAHGSHTLIVKINNPNIKIDGNSNYITTSFYYGSPPNYDWIFYVFAVVLGVMIIMVMGSGRRASQPKWKRTKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-20" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q979E6</accession>
    <name>IPYR_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="1">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="1">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ppa</name>
        <name type="ordered locus">TV1215</name>
        <name type="ORF">TVG1246465</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60357.1"/>
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        <property type="project" value="InterPro"/>
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    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="169"/>
        </location>
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            <position position="26"/>
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        <ligand>
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        <ligand>
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        <ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
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        <ligand>
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            <label>1</label>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
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        <location>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
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    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <sequence length="169" mass="19365" checksum="3D3B254437156C29" modified="2001-10-01" version="1">MKNKQTEDFPESFYVMIEIPMGSNCKYEYKEELDNIVLDRVLFTAMTYPANYGFALDTRGKDGDPLDVLVFSSVSINPGIIVRCRAIGVAEMNDEEGEDNKILAVPVDKVDPASSIVRNESDIPEYQKDKLKHFFEHYKELEKGKFMKFHGFKGRDEAVKQLAEARLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="170" mass="18934" checksum="AAADF1EE6FD7F1CE" modified="2001-10-01" version="1">MLSRNATVAISVIVLIVFAGVAYEVIHNSSSTSETLKISGNIKVKPDLKPSLTKTVNNTTYPIYLVSQNTNYIYLNFTLNSTSNTVYVYDISPLNSSTYSWTNLTFFNGTGNYVELANVSSVPINYSLKLYINKEAIPMMKPDIPYDVVILFISDQGVSTYLPLIFMTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q979H5</accession>
    <name>Q979H5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">L-carnitine dehydratase</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="ORF">TVG1215416</name>
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    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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            <taxon>Thermoplasma</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
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        <property type="entry name" value="CoA-Trfase_fam_III"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
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        <property type="entry name" value="CoA_transf_3"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="399" mass="43773" checksum="C3941A3785DADFC6" modified="2001-10-01" version="1">MDKPLSGIKIIDFTRAMSGPFATMILGDLGAEVIKVESPEGDETRSWVPPMMNGQSAYFLSVNRNKKSIVVDLKKSEGRDIALRLIEGSDIVVENFRPGVAEKLGISYEDAKRVNQNVIYCSISGYGSTGPYMGLSGFDLSVMAFSGLMSITGEPNGGPVKFGVPIVDIVTGMFAAISIISALRFRDKTGEGQYIDTAMIDSAVGILTHQATYFFATGKDPVKLGSAHSSIAPYQAFRTKDGYIVITAGSQKIWENLCDAIEMPELKQDPRFMTNELRVKNRYELVPVIEQYLTRLSTSQAYSKLTKYGVPCSPVNTVSEALKSDQVNYRDMVVGVFSDIYGKVKLINSPLKMSRTPGQIFSAPPVLGQHTIDVLKEFGFRSEEISNLIDSKTIIAYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q979I4</accession>
    <name>Q979I4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo / keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1206711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917410.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979I4"/>
    <dbReference type="STRING" id="273116.gene:9381978"/>
    <dbReference type="PaxDb" id="273116-14325415"/>
    <dbReference type="GeneID" id="1441292"/>
    <dbReference type="KEGG" id="tvo:TVG1206711"/>
    <dbReference type="eggNOG" id="arCOG01619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_3_2"/>
    <dbReference type="OrthoDB" id="275427at2157"/>
    <dbReference type="PhylomeDB" id="Q979I4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19072">
        <property type="entry name" value="AKR_AKR3F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43638:SF3">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43638">
        <property type="entry name" value="OXIDOREDUCTASE, ALDO/KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="38"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="288" mass="32881" checksum="A3C6BE04837BD3FC" modified="2001-10-01" version="1">MDCLITEMEYKEFGNTGVKVSSVGIGTYYDPGWMALSRFNVKRDAEKKIQAIRTAIDAGVTLIDTAEIYGSEDLVAKAIEGHNREELFIATKVWINHLRYDKVIKACKASLSRLNTKYVDLYQIHFPSPTANLQETFKAMEKLVDDGLVKFIGVSNFSRGKLEKSMEYVKKYEIVSNQIHYSLKKRDAEEDIVKFCENNGMAVLAWYPLEHGSLVRESDYPSEILARIKAKHPSITPAQIALSYLIYGNKLVFPIPRASNPEHVKENIASVEQKLDQEEIEELRKYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q979J5</accession>
    <name>Q979J5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP:glycerol 3-phosphotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1192129</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917399.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979J5"/>
    <dbReference type="STRING" id="273116.gene:9381967"/>
    <dbReference type="PaxDb" id="273116-14325404"/>
    <dbReference type="GeneID" id="1441281"/>
    <dbReference type="KEGG" id="tvo:TVG1192129"/>
    <dbReference type="eggNOG" id="arCOG00024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_3_2"/>
    <dbReference type="OrthoDB" id="26592at2157"/>
    <dbReference type="PhylomeDB" id="Q979J5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="7">
        <location>
            <begin position="12"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="8">
        <location>
            <begin position="268"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="498" mass="55479" checksum="B14F8EF7D3A79D3A" modified="2001-10-01" version="1">MARRPIDSDDIFLALDAGTTDVKAGAYDRSMNLIASCKRRIGVYYGQGGVVEQDPHEILEAAKYCLNSILRRIPKRYGEPKAIGITNQRESVLAWEPIDGRPITKLISWKDKRGAQLSLDLKERYGQVIKDKTGLISDPYFSATKIKWLVENIKRSSNHKNYVITTLDSWLVKNLNSSSIKPLTDHSNASRTMLFNIDSLEWDSDLLEIVGISEEILPEVKRTIESDSYGKVKLTYGSTTREVPILSVAGDQQASLFGNGCLYPGQAKASYGTGAFILENTGVRVKSDRLLETLFYTYKGKRTYALEGTILSSGSSIDWLVSLAGLKSVPRSILRAEQIERSHVLSVPALSGLGSPFYSSDIRGYISGLSESTDIYEVIRSFLEAQAFLSTVIIEEMRKHIKLLEPLHIDGGLSKSDLIAEMLANLLDMKIIRERNVDATMKGIAMMSMIGYYGIDERKLTVSAGGKEFNPNDKRDEIAEKFNSWRSFLLSVISKNQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q979M3</accession>
    <name>Q979M3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH dehydrogenase I chain D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1164251</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979M3"/>
    <dbReference type="STRING" id="273116.gene:9381936"/>
    <dbReference type="PaxDb" id="273116-14325375"/>
    <dbReference type="KEGG" id="tvo:TVG1164251"/>
    <dbReference type="eggNOG" id="arCOG01548">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_2"/>
    <dbReference type="PhylomeDB" id="Q979M3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01358">
        <property type="entry name" value="NDH1_NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004739">
        <property type="entry name" value="PRK06075.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 49 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="2">
        <location>
            <begin position="120"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="369" mass="42051" checksum="C996B123EA662205" modified="2001-10-01" version="1">MVMEEWTEVNFGPQHPSMHGVLRLKVKLDGEIVKDVEPIIGYLHRNAEKICELQFYCDNMIYFDRMDYVAAMNMEIGYLEAAEKLLDVEPPGRAKWIRVMMGELNRIASHLVWLGAFGLDLGMLTPFFYCFKEREKILKIFTEVSGSRQQLNYMSIGGVYQDVTDEALDHIKQFITEFPKELNMIANLFVKNDIFLSRTKGVGILSKEMAIDYGVTGPMLRASGVEYDVRKAEPYLVYDKVNFDIPVSFKGDNLARFLVRIEEMRQSVKIIDQTIQKLPAGPYFNQKAKKPLMIRLRGQGEVYARTESPKGEFGVYIVGDGSLRPYRVRVRSPTFKNLSVLPALAKGQMIADLISIGGSIDLVFGEVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q979N4</accession>
    <name>Q979N4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1156448 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1156448</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917360.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979N4"/>
    <dbReference type="STRING" id="273116.gene:9381925"/>
    <dbReference type="PaxDb" id="273116-14325364"/>
    <dbReference type="GeneID" id="1441242"/>
    <dbReference type="KEGG" id="tvo:TVG1156448"/>
    <dbReference type="eggNOG" id="arCOG05405">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2565913_0_0_2"/>
    <dbReference type="OrthoDB" id="55835at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="81" mass="9769" checksum="59C8D45DCBD15B76" modified="2001-10-01" version="1">MSFEECFNKVEDEEEAAECIHCLKKYGEQIMFDEDHGRLVLGREIYDGRFKGQMENLTKLLGIRNRKDYEYVDKKYNLTMY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-29" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q979P6</accession>
    <name>SYD_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate--tRNA(Asp) ligase</fullName>
            <ecNumber evidence="1">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">D-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aspS</name>
        <name type="ordered locus">TV1114</name>
        <name type="ORF">TVG1147365</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917348.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979P6"/>
    <dbReference type="SMR" id="Q979P6"/>
    <dbReference type="STRING" id="273116.gene:9381913"/>
    <dbReference type="PaxDb" id="273116-14325352"/>
    <dbReference type="GeneID" id="1441230"/>
    <dbReference type="KEGG" id="tvo:TVG1147365"/>
    <dbReference type="eggNOG" id="arCOG00406">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_1_2"/>
    <dbReference type="OrthoDB" id="5908at2157"/>
    <dbReference type="PhylomeDB" id="Q979P6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000038">
        <property type="entry name" value="Aspartate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000111009" description="Aspartate--tRNA(Asp) ligase">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="1">
        <location>
            <begin position="188"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="218"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="399"/>
            <end position="402"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA discrimination" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <sequence length="428" mass="49308" checksum="946C0E5B78A0297A" modified="2001-10-01" version="1">MPRVYIGSLRELNHNDNVEIYGWLQDLKLLKNVSFLIMRDRTGTVQVTFKNTPDIVNLLKELPRESVLKISGKINKKSVSKSGLEVSGESVEVLNRSERPLPLPVIDPVQADLETRLNNRFIDLRKRDVSSIFNAESSILWGIREFLHSQGFIEVHTPKIVAAATEGGADLFPVKYFENDAYLNQSPQLYKEILMSSGFEKVFEVGPAFRAEEHNTTRHLNEFTSIDIEMSFADHNDAMRILENAVKSGIENMINENGKDLQENGINISIPEIPFPRITYKECIKLLEKEGLEFQFGNDFSPEELRIIGRNFKDFYFITEWPSSLRPFYTMPNRDDPTITNSFDLQYREVEVTSGAQRVHDPDLLLKRFNEKKLNIDSFKFYIQAFKYGMPPHAGWGLGLERLTMIVLGLNNIRETTLFPRDRTRIIP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-09" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q979S5</accession>
    <name>ALBA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA/RNA-binding protein Alba</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">albA</name>
        <name type="ordered locus">TV1085</name>
        <name type="ORF">TVG1115434</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds double-stranded DNA tightly but without sequence specificity. Involved in DNA compaction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Acetylated. Acetylation at Lys-11 decreases DNA-binding affinity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917316.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="4Z9E">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.49 A"/>
        <property type="chains" value="A/B/C/D=1-89"/>
    </dbReference>
    <dbReference type="PDBsum" id="4Z9E"/>
    <dbReference type="AlphaFoldDB" id="Q979S5"/>
    <dbReference type="SMR" id="Q979S5"/>
    <dbReference type="STRING" id="273116.gene:9381881"/>
    <dbReference type="PaxDb" id="273116-14325323"/>
    <dbReference type="GeneID" id="1441198"/>
    <dbReference type="KEGG" id="tvo:TVG1115434"/>
    <dbReference type="eggNOG" id="arCOG01753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110989_1_0_2"/>
    <dbReference type="OrthoDB" id="10360at2157"/>
    <dbReference type="PhylomeDB" id="Q979S5"/>
    <dbReference type="EvolutionaryTrace" id="Q979S5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00285">
        <property type="entry name" value="DNA-binding protein Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003088">
        <property type="entry name" value="PRK04015.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0007">Acetylation</keyword>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0226">DNA condensation</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151717" description="DNA/RNA-binding protein Alba">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="6"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="26"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="38"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="80"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="4Z9E"/>
        </source>
    </evidence>
    <sequence length="89" mass="9966" checksum="067DFE3A27A3FAB9" modified="2001-10-01" version="1">MAEENIIFVGKKPTMNYVLAVVTQFNNNANKIIIKARGKTISKAVDVAEITRHKFIPDAKYEEIRLDTETLQGERGSSNVSSIEITLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q979S8</accession>
    <name>Q979S8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1110903</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917312.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979S8"/>
    <dbReference type="STRING" id="273116.gene:9381878"/>
    <dbReference type="PaxDb" id="273116-14325320"/>
    <dbReference type="GeneID" id="1441194"/>
    <dbReference type="KEGG" id="tvo:TVG1110903"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_2_2"/>
    <dbReference type="PhylomeDB" id="Q979S8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07130">
        <property type="entry name" value="ALDH_F7_AASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000018">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521:SF1">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="29"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="514" mass="56358" checksum="B6E46A7E25D0B355" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q979T7</accession>
    <name>Q979T7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Origin recognition complex protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1100789</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917302.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979T7"/>
    <dbReference type="SMR" id="Q979T7"/>
    <dbReference type="STRING" id="273116.gene:9381869"/>
    <dbReference type="PaxDb" id="273116-14325311"/>
    <dbReference type="DNASU" id="1441184"/>
    <dbReference type="GeneID" id="1441184"/>
    <dbReference type="KEGG" id="tvo:TVG1100789"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_3_2_2"/>
    <dbReference type="OrthoDB" id="195574at2157"/>
    <dbReference type="PhylomeDB" id="Q979T7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="41"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="369" mass="41581" checksum="306537A318FE0D3A" modified="2001-10-01" version="1">MVILLNPNILESSFIPERLRARDSEISKIMEVVIKPALNNITTNLIIYGDSGTGKTVTMRFLAREVRNPKIFYINAISYRSVKNVLVELLSHEGVIISERASYANIYTRLEKAIEKYDKTVILVIDEAANILRTDEEGLYYLFRSKDSFDVNISAIFIAMDDPALLLNQRIKRSYGLFNELKFKRYSKDEILEIVRDRARMSLNTTSYDDTIIDYIAEISSEFGSARVAIDILAKAAHIAEYRRSENISYDDVRAAKSMISPYVTESKLASLDKYELAALLAICGCLSHSVSTDIACVSEQLKILREQYSLDPEMDIYEILRKLEGLGIINSYVESSGKSSGVRKKISIHDVPVSVLAEKIGNLLNGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q979U6</accession>
    <name>Q979U6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1089125</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917293.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979U6"/>
    <dbReference type="STRING" id="273116.gene:9381858"/>
    <dbReference type="PaxDb" id="273116-14325302"/>
    <dbReference type="GeneID" id="1441175"/>
    <dbReference type="KEGG" id="tvo:TVG1089125"/>
    <dbReference type="eggNOG" id="arCOG01139">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116578_1_0_2"/>
    <dbReference type="OrthoDB" id="4612at2157"/>
    <dbReference type="PhylomeDB" id="Q979U6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08072">
        <property type="entry name" value="MPN_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="122" mass="13899" checksum="3067A90676208BFC" modified="2001-10-01" version="1">MWLIKKDTLQMIMEASKDSYPREFGALLRAEDNVIYEIALVPGTIQSDRYTLFYMYSKPIDFSLVGSVHSHPSGITKPSDEDLRMFSLTGKIHIIVGYPYNLKDYSAYDRSGNKVPLEIIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q979V1</accession>
    <name>Q979V1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Demethylmenaquinone methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.163</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">menG</name>
        <name evidence="5" type="ORF">TVG1083723</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2-demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9539"/>
            <dbReference type="Rhea" id="RHEA-COMP:9563"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18151"/>
            <dbReference type="ChEBI" id="CHEBI:55437"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.163"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Quinol/quinone metabolism; menaquinone biosynthesis; menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.163" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917288.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979V1"/>
    <dbReference type="STRING" id="273116.gene:9381853"/>
    <dbReference type="PaxDb" id="273116-14325297"/>
    <dbReference type="DNASU" id="1441170"/>
    <dbReference type="GeneID" id="1441170"/>
    <dbReference type="KEGG" id="tvo:TVG1083723"/>
    <dbReference type="eggNOG" id="arCOG04348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_0_0_2"/>
    <dbReference type="OrthoDB" id="30774at2157"/>
    <dbReference type="PhylomeDB" id="Q979V1"/>
    <dbReference type="UniPathway" id="UPA00079">
        <property type="reaction ID" value="UER00169"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043770">
        <property type="term" value="F:demethylmenaquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="223" mass="25468" checksum="C333084246EFEAAF" modified="2001-10-01" version="1">MISKHQAVKKIFDQISDKYDLIDTIISLGLDQRWRRSLISNLKLMPGLQCLDCGAGSGKVTELIMESCDSCSVTALDITDSMFNPELTKRCKFVVSPAESLPFSDETFDRVSSAFLTRNLADVDKYFSEVYRVLKPGGIFVNLDIFNPTKPVFSQFFSLYFYRMVPFFGNHATHSRNYSYLANSVKFFYSPFILSKKLESKGFIVEPKDLFFGSVFMHIAQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Z7</accession>
    <name>Q979Z7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1034191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Z7"/>
    <dbReference type="STRING" id="273116.gene:9381806"/>
    <dbReference type="PaxDb" id="273116-14325251"/>
    <dbReference type="DNASU" id="1441123"/>
    <dbReference type="KEGG" id="tvo:TVG1034191"/>
    <dbReference type="eggNOG" id="arCOG01684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117482_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20487" checksum="C699E682EE68F0C9" modified="2001-10-01" version="1">MLTQFERSYALELSRSIGLSQQAILKQLEMLERANLVTSIGFVPSSVGAKRKLYEPTGFSTLIVDYGRNFLMIQRIPLENDSERVSVSFDEAISKLESIQHHIEELTKKRTELVKKKDELMAAIKYQLEDEDPITREIVLEYLNTFDVEEVSRRTALPLEIVLKILKSKGIVLDEGTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A13</accession>
    <name>Q97A13_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1020335 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1020335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A13"/>
    <dbReference type="STRING" id="273116.gene:9381789"/>
    <dbReference type="PaxDb" id="273116-14325214"/>
    <dbReference type="KEGG" id="tvo:TVG1020335"/>
    <dbReference type="HOGENOM" id="CLU_2178031_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="109" mass="13324" checksum="739FC313C74997EA" modified="2001-10-01" version="1">MHFLTFIKSIYNYRIIIGYVYAIKYIIEGESCFGIFHVRHNKLKRTPRYNMYRLAWTVFVCIVRSWRVVYNNKIARETTVYIERDAIDRCVYLNLRHIISIKNSYCRNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A20</accession>
    <name>Q97A20_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1011675 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1011675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A20"/>
    <dbReference type="STRING" id="273116.gene:9381782"/>
    <dbReference type="PaxDb" id="273116-14325207"/>
    <dbReference type="DNASU" id="1441101"/>
    <dbReference type="KEGG" id="tvo:TVG1011675"/>
    <dbReference type="eggNOG" id="arCOG05398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1036730_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="312" mass="36180" checksum="1159C14B28571278" modified="2001-10-01" version="1">MWEYRDMMKRMVYPVRIDTIRYDRKFSFVVFVEDIQSSHQIIDNIMDIGDEIGYPYEVIVSAKDQVTDDYYSDEIKVVGTGLNRRSDGIQRSLKSSTGNYFVIFDPNIRYSLSYADLLYGYAETGSSEVLVSEILGFQTDVLRSVGGWRDLSYGEDIDLLVRVIDNYGVITYPFYGLKQIDLTELIKSTDSIRSGKIKKLRDLIISCNFKYQDIKTIAKILDKSSRISRLLYLSKILSNFARIRPYERPKNNYLTIMERIMESILAKEFLKITTVDFKPSLAISTKTAEYLAGESPLWRITEENGNFVREKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A32</accession>
    <name>Q97A32_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translation initiation factor eIF2 beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0999664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917207.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A32"/>
    <dbReference type="STRING" id="273116.gene:9381770"/>
    <dbReference type="PaxDb" id="273116-14325195"/>
    <dbReference type="GeneID" id="1442055"/>
    <dbReference type="KEGG" id="tvo:TVG0999664"/>
    <dbReference type="eggNOG" id="arCOG01125">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092757_0_0_2"/>
    <dbReference type="OrthoDB" id="27639at2157"/>
    <dbReference type="PhylomeDB" id="Q97A32"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004994">
        <property type="entry name" value="PRK06372.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="253" mass="27966" checksum="F68EBF6DF865E7F3" modified="2001-10-01" version="1">MDMKDLLSDNKSGSTDIAIKLLSLYEDGKLDNSAVEYLYNAFSGMGLVRNIISRLRSGDKVQPHEVKLELKKEEESAVKLAVTEFPFKKVATISAGSMLKKFVTSSKSLDTLYILESRPMLEGSAMAKELKSSGIRAIVLTDASMCVAVENANAVIVGSDSILGDMTLIHKIGTFPLALCARRLGKPFISLSISMKYEEKFTYNTYPGFQLHSCNEIAEGIDCINKYFDKTPRDLIDYIVMEDRIMTSKSSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A62</accession>
    <name>Q97A62_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Precorrin-3b c17-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0973788</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760328.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A62"/>
    <dbReference type="STRING" id="273116.gene:9381740"/>
    <dbReference type="PaxDb" id="273116-14325165"/>
    <dbReference type="DNASU" id="1442027"/>
    <dbReference type="GeneID" id="1442027"/>
    <dbReference type="KEGG" id="tvo:TVG0973788"/>
    <dbReference type="eggNOG" id="arCOG00647">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047948_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q97A62"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051810">
        <property type="entry name" value="Precorrin_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036:SF1">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="250" mass="27405" checksum="6CF16FA8A385BB99" modified="2001-10-01" version="1">MSSLFVVGIGPGSNDLMTPQASLAIERSEYVIGFSLYVSFIKEKFPNKIYISNGMQGEIERAKKAIELARSGHNVSIISSGDAGIYGIAGAVFEILSTLEDNMPEVSVVPGISALSSCASLLGSPLSNDFMVLSLSDLLTPWEVIRKRAEAAAILDLVTVIYNPRGSRFPNNLEKVMEYFLKYRSGDTPVGIVRNAYRPDQSAKIVQLKDFSCSEIDMLTTVIIGNSETYVSGRWMATRRGYSGKYDVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A77</accession>
    <name>Q97A77_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0960602 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0960602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A77"/>
    <dbReference type="STRING" id="273116.gene:9381725"/>
    <dbReference type="PaxDb" id="273116-14325150"/>
    <dbReference type="KEGG" id="tvo:TVG0960602"/>
    <dbReference type="HOGENOM" id="CLU_3113287_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="50" mass="6085" checksum="B42D5C3F67E42878" modified="2001-10-01" version="1">MQDSANIKMANRELLEVFRQIFFEKVESLIIIKLSRNIQKEELTKYKRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AA4</accession>
    <name>Q97AA4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Galactokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0927911</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the GHMP kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917146.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AA4"/>
    <dbReference type="STRING" id="273116.gene:9381698"/>
    <dbReference type="PaxDb" id="273116-14325123"/>
    <dbReference type="GeneID" id="1441994"/>
    <dbReference type="KEGG" id="tvo:TVG0927911"/>
    <dbReference type="eggNOG" id="arCOG01030">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048558_1_0_2"/>
    <dbReference type="OrthoDB" id="116110at2157"/>
    <dbReference type="PhylomeDB" id="Q97AA4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050201">
        <property type="term" value="F:fucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042352">
        <property type="term" value="P:GDP-L-fucose salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052203">
        <property type="entry name" value="GHMP_Kinase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001174">
        <property type="entry name" value="HddA/FKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014606">
        <property type="entry name" value="Heptose_7-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32463">
        <property type="entry name" value="L-FUCOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32463:SF0">
        <property type="entry name" value="L-FUCOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036406">
        <property type="entry name" value="Hept_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00960">
        <property type="entry name" value="LMBPPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="6">
        <location>
            <begin position="79"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="7">
        <location>
            <begin position="226"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="324" mass="36350" checksum="A556C37F6A3EAABF" modified="2001-10-01" version="1">MIITKTPLRITFVGGGTDIPEYYRKKGGAVVSAAINKYIYVIVNKKFDNKIRISYSKTEMVDSVEDIEHPSVRESLKLLGIDGGIEILSISDIPSKGTGLGSSSTFLVGLLNALHAYKGELTSREELAREAVLIEREILKEPGGKQDQYMAAYGGINLMNFNQDESVYVRPLSINAKSLEDFRNHLLLLYTGIQRNSTDIHKKQREEVIKKEEYYDEMKELAYTFFEAVYKAEYPRIGAIMDANWKNKKKLTDGISNDIIDNLYELAMKKGAYGGKLIGAGGGGFLLFVCEPSDSDDIIKALSLRKIDFDFDFEGSRIIYVSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AB0</accession>
    <name>Q97AB0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0921852 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0921852</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917140.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AB0"/>
    <dbReference type="PaxDb" id="273116-14325117"/>
    <dbReference type="DNASU" id="1441988"/>
    <dbReference type="GeneID" id="1441988"/>
    <dbReference type="KEGG" id="tvo:TVG0921852"/>
    <dbReference type="eggNOG" id="arCOG09146">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840694_0_0_2"/>
    <dbReference type="OrthoDB" id="194318at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="139" mass="15504" checksum="461B1E0B4DCAAD9A" modified="2001-10-01" version="1">MAHLNSTIIAHANQSSMNRFLSSNIDTDLMFMKTLENINSVEDDGILAIDDIIKKTGKNIEAAGWIFDHSTGRTVWGIQFATSVLSGRYGIYPISAEVYMIKESLDDENEYRSKIDIQKGVIEKCFTDGLNFSTVTDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AE1</accession>
    <name>Q97AE1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0887956 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0887956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917113.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AE1"/>
    <dbReference type="STRING" id="273116.gene:9381661"/>
    <dbReference type="PaxDb" id="273116-14325086"/>
    <dbReference type="GeneID" id="1441961"/>
    <dbReference type="KEGG" id="tvo:TVG0887956"/>
    <dbReference type="eggNOG" id="arCOG03851">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165897_0_0_2"/>
    <dbReference type="OrthoDB" id="57238at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="107" mass="12430" checksum="7F27203926620633" modified="2001-10-01" version="1">MEPFYFKSYDKLIGVACDEESLLQEMKCLSKFDSAAVYYHLVEGHISMWLEYIGRRDLADGLKYISDVQEAVSYLEKNLRKGSVMPRKRGRPKGSSKKKDMQEKKPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AG1</accession>
    <name>Q97AG1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyruvate ferredoxin oxidoreductasse [POR] alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0871982</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917093.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AG1"/>
    <dbReference type="STRING" id="273116.gene:9381641"/>
    <dbReference type="PaxDb" id="273116-14325066"/>
    <dbReference type="GeneID" id="1441941"/>
    <dbReference type="KEGG" id="tvo:TVG0871982"/>
    <dbReference type="eggNOG" id="arCOG01608">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002569_5_0_2"/>
    <dbReference type="OrthoDB" id="372068at2157"/>
    <dbReference type="PhylomeDB" id="Q97AG1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000010">
        <property type="entry name" value="Pyruvate ferredoxin oxidoreductase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000012">
        <property type="entry name" value="Pyruvate:ferredoxin (Flavodoxin) oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF0">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="3">
        <location>
            <begin position="20"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="4">
        <location>
            <begin position="267"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="392" mass="44074" checksum="07271A4D994B89F0" modified="2001-10-01" version="1">MKSMQDYRTIMTGNDAVAYGAKLARVKLISAYPITPQTTIVEKLASMIANDELDAKYVKVESEHSALATVVSSELAGVRSYTATSSHGLLYMHEMLHWASGQRLPIVMSVVNRAIGPPWNIWADQSDTMNQKDTGWMQVYCESNQEALDTIIMGYKIAENKDVLLPLMSMEDAFILSHTSEPVTIRDQESVNEYLPDLDLPFKINEEHPMGYGSYDTPDGSFMELKKDMVDSMQKAKRIIEDETRKFNEMFGTEYGGLIEKYRMEDADYAIIATGTIASTARYVVDILRKKGEKVGLIRIRYFRPFPYEEILSSLKYVKAAGVIDRSVSFSSGGPTYVEIVQGLYGKLNVPLSSFIAGIGGRDVRIQDIENIYEMLKSGNSGTYWINVKEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AH8</accession>
    <name>Q97AH8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0848765</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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                <person name="Higuchi S."/>
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                <person name="Yamazaki M."/>
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        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="497"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="590" mass="64925" checksum="4675C922E43B9649" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AJ2</accession>
    <name>Q97AJ2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Endonuclease III</fullName>
            <ecNumber evidence="12">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nth</name>
        <name evidence="14" type="ORF">TVG0824522</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine.</text>
            <dbReference type="EC" id="3.2.2.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="12"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917062.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AJ2"/>
    <dbReference type="STRING" id="273116.gene:9381608"/>
    <dbReference type="PaxDb" id="273116-14325034"/>
    <dbReference type="GeneID" id="1441910"/>
    <dbReference type="KEGG" id="tvo:TVG0824522"/>
    <dbReference type="eggNOG" id="arCOG00459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_3_2"/>
    <dbReference type="OrthoDB" id="84708at2157"/>
    <dbReference type="PhylomeDB" id="Q97AJ2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141016">
        <property type="term" value="F:G/T mismatch-specific thymine-DNA glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001435">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0408">Iron</keyword>
    <keyword evidence="7 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="13">
        <location>
            <begin position="39"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB59960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="215" mass="24400" checksum="A3B144794CA4FD53" modified="2001-10-01" version="1">MNNWDEKSIRDVYERIKDQAPKHKFVFHDPFWMLITTVLSQRTKDETTDQAALALYERYRTIEGLASADVSDVGSIISKVGFWRVKAKKIIMIAQIIRDEYGSKVPASMDQLLSLPGVGVKTASVVLAEGLGIPMIAVDTHVFRISHRIGWSSSKTPEQTAQDLMQIIPKDLWIGFNPTLVEFGKAVCRPVSPKCSMCRINEFCEYYKKKNSKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AK8</accession>
    <name>Q97AK8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0802745 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0802745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917046.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AK8"/>
    <dbReference type="STRING" id="273116.gene:9381592"/>
    <dbReference type="PaxDb" id="273116-14325018"/>
    <dbReference type="GeneID" id="1441894"/>
    <dbReference type="KEGG" id="tvo:TVG0802745"/>
    <dbReference type="eggNOG" id="arCOG07381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1763959_0_0_2"/>
    <dbReference type="OrthoDB" id="57269at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="147" mass="16760" checksum="1893FC363EBAFE95" modified="2001-10-01" version="1">MADTLEKAIDDTILQIKAMMLIAGNDKTSINRIRRTFQTSKDQNLMEFRQIVGQQENHNDLIYAAIGEFFLSAFMILLSVIMMVPSLFSFVNTQLSYNYLRSFALNVVKFNTAGYIEIIATLAIAFTILFLALHTIRLAAEKINNLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AM2</accession>
    <name>Q97AM2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Guanylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0790954</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917032.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AM2"/>
    <dbReference type="STRING" id="273116.gene:9381578"/>
    <dbReference type="PaxDb" id="273116-14325004"/>
    <dbReference type="GeneID" id="1441880"/>
    <dbReference type="KEGG" id="tvo:TVG0790954"/>
    <dbReference type="eggNOG" id="arCOG01226">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043725_1_0_2"/>
    <dbReference type="OrthoDB" id="21324at2157"/>
    <dbReference type="PhylomeDB" id="Q97AM2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03114">
        <property type="entry name" value="MMAA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052040">
        <property type="entry name" value="GTPase/Isobutyryl-CoA_mutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005129">
        <property type="entry name" value="GTPase_ArgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00750">
        <property type="entry name" value="lao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087:SF1">
        <property type="entry name" value="LAO_AO TRANSPORT SYSTEM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087">
        <property type="entry name" value="LYSINE/ARGININE/ORNITHINE TRANSPORT SYSTEM KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03308">
        <property type="entry name" value="MeaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="45"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="315" mass="34840" checksum="FE407C796309FFC1" modified="2001-10-01" version="1">MDKSTLVEYIRKGEPRAIARAISLVEEDNDYSYELIREIYPYTGKAHIIGITGPPGVGKSTMIGILSKMLSEHGKKVSILAVDASSPFSGGTLLGNRIRMQEILSKYGIYMRSVANRGFTGGLSRSTWNIVKVLDASGSDYIIIETVGAGQSDIDIVYLADTVIVTLAPGLGDSIQAIKSGMMEIGDIFVINKMDREGSFFALKDIMDNVYEHNGWNPRVVGTNSLKGEGYDKLLEAIDEHRKYSEASGNKKEIRYKEEIRLAISREIEKKVNGAFGELISAEDIARDMRMKIDPSTVARRLFHSSNVAEKEVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AM7</accession>
    <name>Q97AM7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cas12f1-like TNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0786138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AM7"/>
    <dbReference type="SMR" id="Q97AM7"/>
    <dbReference type="STRING" id="273116.gene:9381573"/>
    <dbReference type="PaxDb" id="273116-14324999"/>
    <dbReference type="KEGG" id="tvo:TVG0786138"/>
    <dbReference type="eggNOG" id="arCOG00680">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_5_2"/>
    <dbReference type="PhylomeDB" id="Q97AM7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="154"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="249" mass="28710" checksum="19231178F590D091" modified="2001-10-01" version="1">MKLTLSYYFRNDFEKINQVELGREFAYVSVTIRENPMIEPKAYLVVDRNTTGHIAVVGNPETGNVLKLRKSAEHIHRKYKNIRKHLQKKGKYRMVKKAKDRENRIEKDVNHKVSKKIVQEAKQNGMGIKLEYLNGIRNAKSSRNFRYPLNSWSFYQIEKMIEYKARLLGIPVVYVDPYNTSKECSRCGLKGNRSGKEFKCPGCGHVDNADANASFNIALRPVFEKGIDRLHADRDACKGSTDTHREATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AM9</accession>
    <name>Q97AM9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Malate oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0785282</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AM9"/>
    <dbReference type="STRING" id="273116.gene:9381571"/>
    <dbReference type="PaxDb" id="273116-14324997"/>
    <dbReference type="KEGG" id="tvo:TVG0785282"/>
    <dbReference type="eggNOG" id="arCOG00853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034446_2_1_2"/>
    <dbReference type="PhylomeDB" id="Q97AM9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004470">
        <property type="term" value="F:malic enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051674">
        <property type="entry name" value="Malate_Decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237:SF4">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="3">
        <location>
            <begin position="27"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="2">
        <location>
            <begin position="172"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="451" mass="50113" checksum="CED6564115105546" modified="2001-10-01" version="1">MTTMIMENEERTEKYNEDALRYSEYYKGKIQTMSKVPVRSLDDFSIWYTPGVAAVSRKIATDHDLSFELTGRWNTIAILTDGTRVLGLGNIGPEAAMPVMEGKALIFNYLGGVNAIPVPIRVSSREEFVSVAKSLEPSFGGINLEDIESPKCFFLLETLQKEMNIPVWHDDQLGTASITLAGIINSIRIVGKKIEEVKVVFNGAGAANIAAAYLFKAAGFKKENIILVDSKGVLQPERQDMDSLMLNNPWKYQLAIETNGERVKGDLKEALKGADILISAAKSGPDTVDAKLVKEMNNDPIVFVLANPIPEMWPEEAKENGAKIVATGRSDFPNQINNSLVFPGVFRGVLDARAKGVNFDVMVAASYEIANFVEEPTAEKIVPTMEEWELYPRLASAVATKTVELGLARKIDSKEGFYKRARDIIENNRNIYRKMMDLGLIRMMPNGERKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AN2</accession>
    <name>Q97AN2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0782190 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0782190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AN2"/>
    <dbReference type="STRING" id="273116.gene:9381568"/>
    <dbReference type="PaxDb" id="273116-14324994"/>
    <dbReference type="KEGG" id="tvo:TVG0782190"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2271190_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AN2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="60"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="102" mass="12281" checksum="5B8EB7E13E51E7CB" modified="2001-10-01" version="1">MYNTLTMKAEEVLNLLQISRKTLHVYSKDGRIRYTVMPNKRYNYNEEDVYKILNKDVKRKTVLYERVSTAKQKNDLQNQIDQMKQWCFMNGYTISALLHARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AN9</accession>
    <name>Q97AN9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0774569</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917018.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AN9"/>
    <dbReference type="STRING" id="273116.gene:9381561"/>
    <dbReference type="PaxDb" id="273116-14324987"/>
    <dbReference type="GeneID" id="1441866"/>
    <dbReference type="KEGG" id="tvo:TVG0774569"/>
    <dbReference type="eggNOG" id="arCOG01352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_2"/>
    <dbReference type="OrthoDB" id="6425at2157"/>
    <dbReference type="PhylomeDB" id="Q97AN9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000003">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="183"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="416" mass="45741" checksum="437EAF8EFEEAC685" modified="2001-10-01" version="1">MPESIDPFEMALQQLDKAARVLNLDEQALEILRQPEKILQVSIPVRMDNGKIKVFTGFRVRYNTARGPGKGGIRYHPDETLSTVKALASWMTWKCAIADIPYGGAKGGIICDPKSMSQGELERLSRGYIRAIADFIGPEVDVPAPDVYTNPQIMAWMMDEYENIVRHSAPNVITGKPLEIGGSEGRGDATAKGGMYVLREGAKRVGLDLSKATVAVQGFGNAGQFAVKFVQEMFGAKVVAVSDTKGGIFKSDGINYEALLDHKRKTGSVVGFPGSKPITNEELLESDVDVLIPAAIEEQITEKNADKIKAKIILELANGPTTPEADEILYKKGRLDLPDFLSNSGGVTVSYFEWVQNNYGEYWTAEEVYEKLDKKMTKAAHEVFDAMDKYKINPRTAAYVISVKKVADAMKARGMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AQ7</accession>
    <name>Q97AQ7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0758278 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0758278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916999.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AQ7"/>
    <dbReference type="STRING" id="273116.gene:9381542"/>
    <dbReference type="PaxDb" id="273116-14324968"/>
    <dbReference type="GeneID" id="1441847"/>
    <dbReference type="KEGG" id="tvo:TVG0758278"/>
    <dbReference type="eggNOG" id="arCOG01916">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1307777_0_0_2"/>
    <dbReference type="OrthoDB" id="11143at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018886">
        <property type="entry name" value="UPF0547"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10571">
        <property type="entry name" value="UPF0547"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0547" evidence="1">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="217" mass="24946" checksum="BF0A96AB1FEA15B5" modified="2001-10-01" version="1">MPVYDPDEHELLQEKSSMTRGGEQYFKGTLYLTDKRLIYEEKGHRGLIHAHPSKILLDLPLYLVVNISIAVPKIKLGTKKTLSVEFTTEKGDDRATFVLKDPGKWQSEMMRWTTDAKRRHEQEEKIKSEEEKRREIELARAKAPTANIGMYINAGKKEGAENQNKNFIETTFSEINPERLEKGEETKALPKTKRCPNCGKDIPIDSVYCPYCGFKQP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-11-02" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AR2</accession>
    <name>TRM1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trm1</name>
        <name type="ordered locus">TV0747</name>
        <name type="ORF">TVG0752240</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916994.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AR2"/>
    <dbReference type="SMR" id="Q97AR2"/>
    <dbReference type="STRING" id="273116.gene:9381537"/>
    <dbReference type="PaxDb" id="273116-14324963"/>
    <dbReference type="DNASU" id="1441842"/>
    <dbReference type="GeneID" id="1441842"/>
    <dbReference type="KEGG" id="tvo:TVG0752240"/>
    <dbReference type="eggNOG" id="arCOG01219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010862_5_1_2"/>
    <dbReference type="OrthoDB" id="372177at2157"/>
    <dbReference type="PhylomeDB" id="Q97AR2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00290">
        <property type="entry name" value="tRNA_dimethyltr_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022923">
        <property type="entry name" value="TRM1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000147696" description="tRNA (guanine(26)-N(2))-dimethyltransferase">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Trm1 methyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00290"/>
        </source>
    </evidence>
    <sequence length="342" mass="38304" checksum="029D97E2984F60F1" modified="2001-10-01" version="1">MRITEGSAVIEVPEAYSGPGRRSPGFYNADQVLNRDLTIDLINEFGVEKVLDGFGGSGVRGIRISLETKAKVVIAEISPSSCEIIKQNIAINNAEADLIHDNFQCVSSHIAFDYIDVDPYGSPVPYLDAAIMNVRKGGYLGITATDQSTLTGSTLNKTRRRYGAYIVNDIYRHEMGIRTLIGYIARRAAAFDRYIEPQLSIWNHHYYRVFVKVGKGVNGADKMIENIGYLNKHTTIDNSFKDVNEGPVWLSNLNNAELLEKIRDPRVSKKIEMIKHDNRLLFVDLTDIARKTSLSLPPLAKVMDEIMKHGYEAHRTSFSPTGIKTDCPYAEVSEILKAYAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AR5</accession>
    <name>Q97AR5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0750122 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0750122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916991.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AR5"/>
    <dbReference type="STRING" id="273116.gene:9381534"/>
    <dbReference type="PaxDb" id="273116-14324960"/>
    <dbReference type="DNASU" id="1441839"/>
    <dbReference type="GeneID" id="1441839"/>
    <dbReference type="KEGG" id="tvo:TVG0750122"/>
    <dbReference type="eggNOG" id="arCOG05350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876093_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="330" mass="35912" checksum="22DD4CCB803B61BB" modified="2001-10-01" version="1">MFTLFAIQFFTTAAAVAFLLTELMGGFMLLFDWKNSGQKIMTYVAPIWEVTGTFLAMFVVETDATFAGILIPAAFAFAALLLIFLILFIVRNVAIILGEFAGSRKIFTDRQLYYIYVIATLLISVVFLAFSSAIISGNGVKIDIAGNPVDSLLSVSNAEISFNLASWLAGPGDILFVVSAALIAFGLSPVFYDVDRFSTYGVISSVIGFILGIVSFVQMGVHISPIIIIPAIIIIGVPILYRIGLAKKILTNKALFIVLLIVSIYSLYFTVYPSAMNGKLPLSQIITTGPMVIGTILTTVVGFTLLAFMLSLYVYVNYRSSRNQMTTLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AS9</accession>
    <name>Q97AS9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0736554 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0736554</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053977.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AS9"/>
    <dbReference type="STRING" id="273116.gene:9381519"/>
    <dbReference type="PaxDb" id="273116-14324946"/>
    <dbReference type="GeneID" id="24779927"/>
    <dbReference type="KEGG" id="tvo:TVG0736554"/>
    <dbReference type="HOGENOM" id="CLU_728862_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="379" mass="44232" checksum="9A974D11AE778815" modified="2001-10-01" version="1">MGINIRPEERSENNLTRLNIKYDKGNVETPNRFVNRYDINAKSHLGAHIPLTRIRNLFIYEEPISNKELNSILNTNDYLATLKNKIEDYFIRIENKNCLKAVYIKLTGNVKDYVFKNRDSETSAKIKKFIFDLSSELNTDFYIFQSELLNDNSIKFLNNNIVFSPLFYIKDHKTIQNSLPNWITQESTLVPFISFTYSSYLNANISYQYIIKYLDKIHESDKGIISTDIPRIFSNLASPYGHVSAPHYSSFIISDITAEIYNGGHGNFKKVPNTRIFEKTNLALPKISNKSNNAEIHKNEDSIFGNDENLKSLFWRTLQNQNVDMDIKNNRPAYISRIHESVMTSSEYDNMRNSIKQHDLMNYRKSKMELNKLLENQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AW2</accession>
    <name>Q97AW2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0703225 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0703225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053962.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AW2"/>
    <dbReference type="STRING" id="273116.gene:9381485"/>
    <dbReference type="PaxDb" id="273116-14324913"/>
    <dbReference type="GeneID" id="24779917"/>
    <dbReference type="KEGG" id="tvo:TVG0703225"/>
    <dbReference type="HOGENOM" id="CLU_2730623_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="71" mass="7971" checksum="1E60E35656E75B8B" modified="2001-10-01" version="1">MKPWNNGPEPPHEPYPIQRDPGDVPPDQQYEDIARPIIHTEAAATAKINSKLSHYNSAFYSSNRIIGEIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AX5</accession>
    <name>Q97AX5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0689729 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0689729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AX5"/>
    <dbReference type="STRING" id="273116.gene:9381472"/>
    <dbReference type="PaxDb" id="273116-14324900"/>
    <dbReference type="KEGG" id="tvo:TVG0689729"/>
    <dbReference type="eggNOG" id="arCOG04792">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773305_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004291">
        <property type="entry name" value="Transposase_IS66_central"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03050">
        <property type="entry name" value="DDE_Tnp_IS66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS66 central" evidence="1">
        <location>
            <begin position="31"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="146" mass="17312" checksum="3907F7050AEC47CF" modified="2001-10-01" version="1">MRQNRLHGSGTMEDVENLYNKLVFLLDYDYEHTKSRKFVDNLLKRRKEWLFSFVVHKDVEPTNNRAERALKPSVIYTKTNGGTRSETGDRTYEKLTSVSYTSRLWKSNIVKDGQPEIRKWMGKKYMKKIEGRLDKSMKRRGQASED</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B46</accession>
    <name>Q97B46_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II secretion system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0602920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916871.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B46"/>
    <dbReference type="STRING" id="273116.gene:9381401"/>
    <dbReference type="PaxDb" id="273116-14324828"/>
    <dbReference type="GeneID" id="1441719"/>
    <dbReference type="KEGG" id="tvo:TVG0602920"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_2_2_2"/>
    <dbReference type="PhylomeDB" id="Q97B46"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01130">
        <property type="entry name" value="VirB11-like_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Type IV secretion system protein"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.390.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF14">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="155"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="535" mass="60813" checksum="857A9F0E513BD5BC" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B52</accession>
    <name>Q97B52_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ferripyochelin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0596843</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916864.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B52"/>
    <dbReference type="STRING" id="273116.gene:9381394"/>
    <dbReference type="PaxDb" id="273116-14324821"/>
    <dbReference type="GeneID" id="1441712"/>
    <dbReference type="KEGG" id="tvo:TVG0596843"/>
    <dbReference type="eggNOG" id="arCOG01849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064827_4_0_2"/>
    <dbReference type="OrthoDB" id="10940at2157"/>
    <dbReference type="PhylomeDB" id="Q97B52"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04645">
        <property type="entry name" value="LbH_gamma_CA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
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        <property type="entry name" value="LbH_gamma_CA-like"/>
    </dbReference>
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        <property type="entry name" value="Transf_Hexapept/Carb_Anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
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        <property type="entry name" value="DYNACTIN SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061:SF29">
        <property type="entry name" value="GAMMA CARBONIC ANHYDRASE-LIKE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="169" mass="18290" checksum="130CB262E17178EF" modified="2001-10-01" version="1">MRIGKNVYIAETAVVIGDVDIADGVSIFDSAVLRADLEKIIIGENTNIQDNVTIHTDVGYPTRIGKYVSIGHNAVVHGCTVEDEVLIGMGAIIMNGAQIGHGSIVGAGAVVTKGFKAPEYSVILGLPAKVMRLNQDQISYVKANAEDYIKLRDLYLSGKFERYRKNSDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B67</accession>
    <name>Q97B67_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0579516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B67"/>
    <dbReference type="STRING" id="273116.gene:9381379"/>
    <dbReference type="PaxDb" id="273116-14324806"/>
    <dbReference type="KEGG" id="tvo:TVG0579516"/>
    <dbReference type="eggNOG" id="arCOG01150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075478_0_1_2"/>
    <dbReference type="PhylomeDB" id="Q97B67"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07391">
        <property type="entry name" value="MPP_PF1019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004376">
        <property type="entry name" value="Pesterase_MJ0037"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00024">
        <property type="entry name" value="SbcD_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323:SF1">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="30"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="253" mass="29699" checksum="31B9725B918825CC" modified="2001-10-01" version="1">MEVSMRDIEILRDVFLSDLYCVYLRDISAVVVSDLHLGYEEEMNLHGLFLPRMQRDHVTHIMDKIVERYDPEKIIINGDFKQEFSKNLPSEWDDIIYFINRYDDRDLIFVRGNHDNYLATILSKKNKELLDYYEDDRYFIYHGDKDLSTKKITILGHEHPSLVLRDRVGGIYKLPAFAFNFKKNVIITPAMSFFSSGTDLSQSLLSEEHFTPSLKGMKASSFRIFAITDEFGLVDFGYLEDLRSSEGQQTRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B90</accession>
    <name>Q97B90_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0555376 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0555376</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916826.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B90"/>
    <dbReference type="SMR" id="Q97B90"/>
    <dbReference type="STRING" id="273116.gene:9381353"/>
    <dbReference type="PaxDb" id="273116-14324782"/>
    <dbReference type="DNASU" id="1441084"/>
    <dbReference type="GeneID" id="1441084"/>
    <dbReference type="KEGG" id="tvo:TVG0555376"/>
    <dbReference type="eggNOG" id="arCOG01368">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056115_0_0_2"/>
    <dbReference type="OrthoDB" id="387097at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="263" mass="29756" checksum="99D7150C51EC61B9" modified="2001-10-01" version="1">MAQSEISKREKGRALITVANNVAGFNLWKRLVEEGYDVDAILPSEDKYNLLLSELVSRKNKKYDFIVNTVDRHDYAVVTNKMLKWLYPTEKIIFISSDLVYEPRPGKKKENDNAYPPTMEGKYFLEAESALDVNSDLVLRVGLVTGRCVPNILTNTINLSRANMSIDLSGAVVRNFISNADLSEYIIRLLDKNGIYNASAKSMTPRDFALELCAKINVNCKLRRTTDDKVFDYSMDTTKIELTTGYYTKDIFANISSGLYLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BA7</accession>
    <name>Q97BA7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0539478</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BA7"/>
    <dbReference type="STRING" id="273116.gene:9381335"/>
    <dbReference type="PaxDb" id="273116-14324765"/>
    <dbReference type="KEGG" id="tvo:TVG0539478"/>
    <dbReference type="eggNOG" id="arCOG01136">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065946_2_1_2"/>
    <dbReference type="OrthoDB" id="30609at2157"/>
    <dbReference type="PhylomeDB" id="Q97BA7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02798">
        <property type="entry name" value="tRNA_bind_CsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000165">
        <property type="entry name" value="Chaperone CsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008231">
        <property type="entry name" value="CsaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02222">
        <property type="entry name" value="chap_CsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007494">
        <property type="entry name" value="PRK10089.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007495">
        <property type="entry name" value="PRK10089.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF37">
        <property type="entry name" value="TRNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="4">
        <location>
            <begin position="12"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="114" mass="12701" checksum="532CCDF7D9110B03" modified="2001-10-01" version="1">MNRLKERTDYETFQKLDIRIGRIISVDSFPEARKPAYKLKIYFGDDIGILNSSAQITNYKPEDLLGRLVVAVVNFPPKRVANFISQVLVLGSITKDGVKLLSVDEGAVPGDYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BB7</accession>
    <name>Q97BB7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0528853 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0528853</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916798.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BB7"/>
    <dbReference type="STRING" id="273116.gene:9381325"/>
    <dbReference type="PaxDb" id="273116-14324755"/>
    <dbReference type="GeneID" id="1441056"/>
    <dbReference type="KEGG" id="tvo:TVG0528853"/>
    <dbReference type="eggNOG" id="arCOG04007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2581607_0_0_2"/>
    <dbReference type="OrthoDB" id="55777at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="80" mass="9179" checksum="6F63C82096C26472" modified="2001-10-01" version="1">MQIEDQRNITCGGDPFNYLKSTLKGLSFQLEPGQDVKLIVLKEKFPFDRSVYDGLARTSKFKLIDVQEENGEVSIILRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BC0</accession>
    <name>Q97BC0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0526731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916794.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BC0"/>
    <dbReference type="SMR" id="Q97BC0"/>
    <dbReference type="STRING" id="273116.gene:9381321"/>
    <dbReference type="PaxDb" id="273116-14324751"/>
    <dbReference type="DNASU" id="1441052"/>
    <dbReference type="GeneID" id="1441052"/>
    <dbReference type="KEGG" id="tvo:TVG0526731"/>
    <dbReference type="eggNOG" id="arCOG02877">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029940_1_0_2"/>
    <dbReference type="OrthoDB" id="10436at2157"/>
    <dbReference type="PhylomeDB" id="Q97BC0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10938">
        <property type="entry name" value="CE4_HpPgdA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037950">
        <property type="entry name" value="PgdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561:SF1">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="42"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="299" mass="34876" checksum="669E5727DDDF2996" modified="2001-10-01" version="1">MDREIFVSYSVSANSIAGWVAYGGSESFHDMSRGILAARIGIPRLLRMFRKFCIKSSWYVPGIVMETFPDEVRAIYADGHELGTHGYLHEYAPSMSYDQEERVIAKTAELVKDITGHNPYGHLPSQWEESVNTMRILMNHGYKYARGLEDDDFHPFYASTGRSWPRVDYSKLPETWMKPMNLGEKIDFVEIPINWFLNDGTYFNFNKHVPGKRPMSLSEVLENWKAAFDYVYREYEYAIFPVNIHPDTSGNPEVQIFHEKFISYILEHNGASFVSDSFIAEDFRKRYPFPHEFREKVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BH0</accession>
    <name>Q97BH0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo7</fullName>
            <ecNumber evidence="4">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpo7</name>
        <name evidence="4" type="synonym">rpoE</name>
        <name evidence="6" type="ORF">TVG0469653</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the RNA polymerase complex. Forms a stalk with Rpo4 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">Forms 2 domains with an elongated structure; Rpo4 packs into the hinge region between the 2 domains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BH0"/>
    <dbReference type="SMR" id="Q97BH0"/>
    <dbReference type="STRING" id="273116.gene:9381267"/>
    <dbReference type="PaxDb" id="273116-14324700"/>
    <dbReference type="KEGG" id="tvo:TVG0469653"/>
    <dbReference type="eggNOG" id="arCOG00675">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117966_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BH0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04331">
        <property type="entry name" value="RNAP_E_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04460">
        <property type="entry name" value="S1_RpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00865">
        <property type="entry name" value="RNApol_arch_Rpo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046399">
        <property type="entry name" value="RNApol_Rpo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006333">
        <property type="entry name" value="PRK08563.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="95"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="195" mass="21793" checksum="B3F05D1B46D88370" modified="2001-10-01" version="1">MICMYMMVEDKYIARIPPDMLGEDYDKAVEEVTKRSLEGKLVDVVNEKTGVNEGKYLVISIISTKAVGEGSIVHGDGGVYQEVKYSALVFYPEMQEIVDGEVVSVQKFGAFVKFSSFEGLLHISQIMDDRIDVDLENKRFIGKDTKRDLKVGDKVRVRIVALNLASSSVEDSKIGFTMKQVGLGKLEWLEKEAQA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BH2</accession>
    <name>DPCKG_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP-dependent dephospho-CoA kinase</fullName>
            <ecNumber evidence="1">2.7.1.237</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephospho-coenzyme A kinase</fullName>
            <shortName evidence="1">DPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV0483</name>
        <name type="ORF">TVG0468984</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3'-dephospho-CoA + GTP = GDP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="2.7.1.237"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-dependent DPCK family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59625" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.7.1.237" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59625.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916742.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BH2"/>
    <dbReference type="SMR" id="Q97BH2"/>
    <dbReference type="STRING" id="273116.gene:9381265"/>
    <dbReference type="PaxDb" id="273116-14324698"/>
    <dbReference type="GeneID" id="1441000"/>
    <dbReference type="KEGG" id="tvo:TVG0468984"/>
    <dbReference type="eggNOG" id="arCOG04076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120795_1_0_2"/>
    <dbReference type="OrthoDB" id="15447at2157"/>
    <dbReference type="PhylomeDB" id="Q97BH2"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00590">
        <property type="entry name" value="Dephospho_CoA_kinase_GTP_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007164">
        <property type="entry name" value="GTP-dep_dephospho-CoA_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002249">
        <property type="entry name" value="PRK01160.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732:SF1">
        <property type="entry name" value="GTP-DEPENDENT DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732">
        <property type="entry name" value="UPF0218 PROTEIN TK1697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04019">
        <property type="entry name" value="DUF359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006533">
        <property type="entry name" value="UCP006533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000137622" description="GTP-dependent dephospho-CoA kinase">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="166" mass="18364" checksum="BE723A70EF9E1924" modified="2002-11-01" version="2">MLLRSGSKYIITAEAREEIKKRNGKLVTIDEMVEVSKSTQLDSVGDVTTTNLRRAGAKLFLQIVDLKTKRGESIFQHEEGSITVRNDPGTISFDLIEAIKSSIENNKPTRIEVIGEEDLAVLPIIYYSKNNTVIAYGIPDVGMAALTVTDDLRDHVTEVIAKMAIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BI9</accession>
    <name>Q97BI9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable transposase IS891/IS1136/IS1341 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0455305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BI9"/>
    <dbReference type="SMR" id="Q97BI9"/>
    <dbReference type="STRING" id="273116.gene:9381248"/>
    <dbReference type="PaxDb" id="273116-14324681"/>
    <dbReference type="DNASU" id="1440984"/>
    <dbReference type="KEGG" id="tvo:TVG0455305"/>
    <dbReference type="eggNOG" id="arCOG00683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040997_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BI9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="2">
        <location>
            <begin position="171"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="295" mass="34916" checksum="233713151BC4F79E" modified="2001-10-01" version="1">MKRANAVKLTVDKKTHEKLKELAVVTAKCWNEVNWLRMQQFKKGERVDFSKTEKEVYEKYKQILKVNTQQVARKNAESWRSFFSLVEEKKGKLPKWFKPRPPGYWKDKSGKYKMLIIIRNDRYEIDEEKRIIYLKDFKLSLSFNGKLKWRGKQGRLEIIYNEARRSWYAYIPVEVQNDVKAEDKLKASIDIGIVNSATVYVEDGSWYIFKGGSVLSQYEYYSKRISVAQKTLARHKQGGSREMKLLHEKRKRFLKHALNSMVRKIMEELKKKDVGAVFTAMIAYIALMMIQDMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BM0</accession>
    <name>Q97BM0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NDP-sugar pyrophosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0423096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916693.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BM0"/>
    <dbReference type="STRING" id="273116.gene:9381214"/>
    <dbReference type="PaxDb" id="273116-14324650"/>
    <dbReference type="GeneID" id="1440951"/>
    <dbReference type="KEGG" id="tvo:TVG0423096"/>
    <dbReference type="eggNOG" id="arCOG00663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_2_2_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="PhylomeDB" id="Q97BM0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="314" mass="36508" checksum="7FED341972DFC675" modified="2001-10-01" version="1">MIGAILAGGYGKRLKPITDSIPKALVEIKDNYTIMDRQLFDFRNIGVKEVYILSGYLGNKIEERYGDLYNDMNMYYLREEKPLGTLYSLRNLLDKRSDDDIILRNGDTITDINFLSFIKKAQSQQAFITIHITKMKSPFGIVETFGDQVSEFREKPELDHYINAGLYYIKQDAFPYFYEEYMYRDLETTVFPKLTKLGLVASYHEDALWMGIDSEKDLEKIREEYKNRDDYPWGYRKVAYDDEDAKIEEYFIKTGEKAMMDLDKGAIIKVIDGQGIIDDGEKTGIREGNIRHASGSIYVTAIENCRFEVIRSKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-25" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BN2</accession>
    <name>RL1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl1</name>
        <name type="ordered locus">TV0423</name>
        <name type="ORF">TVG0408391</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S rRNA. Probably involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916680.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BN2"/>
    <dbReference type="SMR" id="Q97BN2"/>
    <dbReference type="STRING" id="273116.gene:9381201"/>
    <dbReference type="PaxDb" id="273116-14324638"/>
    <dbReference type="GeneID" id="1440938"/>
    <dbReference type="KEGG" id="tvo:TVG0408391"/>
    <dbReference type="eggNOG" id="arCOG04289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_4_0_2"/>
    <dbReference type="OrthoDB" id="10382at2157"/>
    <dbReference type="PhylomeDB" id="Q97BN2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000005">
        <property type="entry name" value="50S ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_A">
        <property type="entry name" value="Ribosomal_uL1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023669">
        <property type="entry name" value="Ribosomal_uL1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003244">
        <property type="entry name" value="PRK04203.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125816" description="Large ribosomal subunit protein uL1">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="217" mass="23856" checksum="4829DDFEFBEF34D8" modified="2001-10-01" version="1">MDINDISKAVEAVKQSSPQRKFEESVEIAINLKDIDMTNPKNRINEEILLPNGRGKDVKVVIFGSEELKAKAKGVADYIFGPEDISKFAEDKKAFKKIVNDAYFFIAEATLMANIGKSLGQVLGPRGKMPRPIPPGQDPVPLIKALKNTVKARSRNSLTLHVPVGTRSMDTQKISENIMAILNRITGKLERGHSNIKNVYVKTTMGKAVQVGTGDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BN4</accession>
    <name>Q97BN4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl12</name>
        <name evidence="6" type="ORF">TVG0407122</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916678.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BN4"/>
    <dbReference type="STRING" id="273116.gene:9381199"/>
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    <dbReference type="DNASU" id="1440936"/>
    <dbReference type="GeneID" id="1440936"/>
    <dbReference type="KEGG" id="tvo:TVG0407122"/>
    <dbReference type="eggNOG" id="arCOG04287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BN4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05832">
        <property type="entry name" value="Ribosomal_L12p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022295">
        <property type="entry name" value="Ribosomal_P1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03685">
        <property type="entry name" value="ribo_P1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="61"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="104" mass="11420" checksum="B19D097AA0D2F37E" modified="2001-10-01" version="1">MEYVYGALLLHAAGKEVDEEKLKKVLEDVGVQVDEARLKTLVSGLKDVNIDEVLKNATVAPVAAAPAAPQEKKEEPKKKEEKKKEENKEQEEEEAMSGLSALFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BQ7</accession>
    <name>Q97BQ7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH dependent alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0387048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BQ7"/>
    <dbReference type="STRING" id="273116.gene:9381176"/>
    <dbReference type="PaxDb" id="273116-14324613"/>
    <dbReference type="KEGG" id="tvo:TVG0387048"/>
    <dbReference type="eggNOG" id="arCOG00984">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007207_0_0_2"/>
    <dbReference type="OrthoDB" id="57329at2157"/>
    <dbReference type="PhylomeDB" id="Q97BQ7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14862">
        <property type="entry name" value="Fe-ADH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000003">
        <property type="entry name" value="Alcohol dehydrogenase, iron-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF83">
        <property type="entry name" value="HYDROXYACID-OXOACID TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="2">
        <location>
            <begin position="7"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="181"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="373" mass="40954" checksum="E4957023AFF5A0A4" modified="2001-10-01" version="1">MWYFRTPRIIFGEDSLSFLDDHSSSKILVVTDNFLSNTPILKEVLSHLSGKDIKVFSEISPEPRLSEILKGRELASRLDPDTIIGLGGGSSMDAAKILFALHEMPDLSPFDISPINSIKLHQKSELIEIPTTSGTGSECSWAAVFSDDTEERKNEIASPEIMADYAILDPKLVLDLPKGITVNTATDAITHAIEAYTSQWRNPYSDAMAEKALDLILNNLPTVLVNPKDINARNSVHIGASMAGISFSNSQIGLAHALGHALGAIFKTPHGESVGLYLPHVTEFNYEAAKDRYDKINSFFPDEYRTDKVWETIRSYLNAIGQVTNVSEAGIERSKYEKELGKIVSLASESTGVITNARDASTKDIENIARSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BR6</accession>
    <name>Q97BR6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">TVG0375766</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2008-02" db="PDB data bank">
            <title>X-ray crystal structure of homoserine dehydrogenase from Thermoplasma volcanium.</title>
            <authorList>
                <person name="Osipiuk J."/>
                <person name="Hendricks R."/>
                <person name="Abdullah J."/>
                <person name="Joachimiak A."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS)</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2009-09" db="PDB data bank">
            <title>X-ray crystal structure of Homoserine dehydrogenase from Thermoplasma volcanium.</title>
            <authorList>
                <person name="Osipiuk J."/>
                <person name="Nocek B."/>
                <person name="Hendricks R."/>
                <person name="Abdullah J."/>
                <person name="Joachimiak A."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH NAD(+)</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916643.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="3C8M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-328"/>
    </dbReference>
    <dbReference type="PDB" id="3JSA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A=1-328"/>
    </dbReference>
    <dbReference type="PDBsum" id="3C8M"/>
    <dbReference type="PDBsum" id="3JSA"/>
    <dbReference type="AlphaFoldDB" id="Q97BR6"/>
    <dbReference type="SMR" id="Q97BR6"/>
    <dbReference type="STRING" id="273116.gene:9381166"/>
    <dbReference type="PaxDb" id="273116-14324604"/>
    <dbReference type="DNASU" id="1440901"/>
    <dbReference type="GeneID" id="1440901"/>
    <dbReference type="KEGG" id="tvo:TVG0375766"/>
    <dbReference type="eggNOG" id="arCOG01351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_1_2_2"/>
    <dbReference type="OrthoDB" id="4488at2157"/>
    <dbReference type="PhylomeDB" id="Q97BR6"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97BR6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005002">
        <property type="entry name" value="PRK06392.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331:SF1">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="16 17" id="KW-0002">3D-structure</keyword>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="13">
        <location>
            <begin position="10"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="12">
        <location>
            <begin position="151"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB59531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="16">
        <source>
            <dbReference type="PDB" id="3C8M"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="17">
        <source>
            <dbReference type="PDB" id="3JSA"/>
        </source>
    </evidence>
    <sequence length="328" mass="36624" checksum="549C35F5E51156B6" modified="2001-10-01" version="1">MKTINLSIFGLGNVGLNLLRIIRSFNEENRLGLKFNVVFVADSLHSYYNERIDIGKVISYKEKGSLDSLEYESISASEALARDFDIVVDATPASADGKKELAFYKETFENGKDVVTANKSGLANFWPEIMEYARSNNRRIRYEATVAGGVPLFSFIDYSVLPSRIKKFRGIVSLTINYFIRELANKREFDDVLSEATKLGIVEKNYKDDLTGLDAARKSVILCNHLYGSSYRLSDVFYEGILDQDRSFGKNERLVTETGIVNGKPSAESRIKSLDSNDYLLTLGKGSLGYQLQTDTNGTLNVSDLYDGPYETAGAVMNDLVILSMFTV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BU6</accession>
    <name>RL37_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L37e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl37e</name>
        <name type="ordered locus">TV0359</name>
        <name type="ORF">TVG0350173</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916613.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BU6"/>
    <dbReference type="SMR" id="Q97BU6"/>
    <dbReference type="STRING" id="273116.gene:9381136"/>
    <dbReference type="PaxDb" id="273116-14324574"/>
    <dbReference type="GeneID" id="32154008"/>
    <dbReference type="KEGG" id="tvo:TVG0350173"/>
    <dbReference type="eggNOG" id="arCOG04126">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208825_0_0_2"/>
    <dbReference type="OrthoDB" id="5619at2157"/>
    <dbReference type="PhylomeDB" id="Q97BU6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_eL37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_eL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_eL37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003214">
        <property type="entry name" value="PRK04179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000139742" description="Large ribosomal subunit protein eL37">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="54" mass="6203" checksum="217C44EF5E183607" modified="2001-10-01" version="1">MSNGTAVMGKINNKKTHITCRRCGHHTYNVRTKRCSHCGFPAPRIRSYKWAKAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-11-16" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BW4</accession>
    <name>RS4E_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>30S ribosomal protein S4e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rps4e</name>
        <name type="ordered locus">TV0341</name>
        <name type="ORF">TVG0339087</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916595.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BW4"/>
    <dbReference type="SMR" id="Q97BW4"/>
    <dbReference type="STRING" id="273116.gene:9381118"/>
    <dbReference type="PaxDb" id="273116-14324556"/>
    <dbReference type="GeneID" id="1440853"/>
    <dbReference type="KEGG" id="tvo:TVG0339087"/>
    <dbReference type="eggNOG" id="arCOG04093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060400_0_0_2"/>
    <dbReference type="OrthoDB" id="372073at2157"/>
    <dbReference type="PhylomeDB" id="Q97BW4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003312">
        <property type="entry name" value="PRK04313.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130866" description="Small ribosomal subunit protein eS4">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding">
        <location>
            <begin position="38"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="235" mass="26110" checksum="A57B11448CFE562E" modified="2001-10-01" version="1">MINKTKRLMVPRTVKIPRKTYFWGPTPLPGRHKADHSVTLLTIIRDYLRLSDKEREATRILANGLVKVDGKVVKERKFGVGFMDVIEISGESYRVVYNNQGALVLVSESKDRANMKPLQVKNKVIAPGNKIQLGFHDGRVMVTEDRSISVGDVVIASLPDMKITEIIKMQPGNKAFITGGSHVGETGTISKIEIKESSSANLVHFDEGFTTVKDHVFVIGSPRFTFTMPSGEVYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C17</accession>
    <name>Q97C17_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Arsenical pump membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0299333</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916543.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C17"/>
    <dbReference type="STRING" id="273116.gene:9381062"/>
    <dbReference type="PaxDb" id="273116-14324502"/>
    <dbReference type="GeneID" id="1440801"/>
    <dbReference type="KEGG" id="tvo:TVG0299333"/>
    <dbReference type="eggNOG" id="arCOG00238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043931_1_0_2"/>
    <dbReference type="OrthoDB" id="19068at2157"/>
    <dbReference type="PhylomeDB" id="Q97C17"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01118">
        <property type="entry name" value="ArsB_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000802">
        <property type="entry name" value="Arsenical_pump_ArsB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00935">
        <property type="entry name" value="2a45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011980">
        <property type="entry name" value="PRK15445.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302:SF5">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02040">
        <property type="entry name" value="ArsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00758">
        <property type="entry name" value="ARSENICPUMP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="431" mass="46930" checksum="CDC0F3102F6356D5" modified="2001-10-01" version="1">MQDFRFVAAAIVFIFTLILVTVRPWKIDIGYGALIGAFVSIILGISTPADVIKVWNIIWNPTFTFVAIIAMSLVLDYAGFFEYYAIRISKFAGGNGIKLFVFIVILGSLTAAFFANDGAVLVLTPIVLSIMDMTGIGKKGAVAYIMAIGFISDSSSLPFVISNLVNIIGSGYFGISFASYAKVMVIPDIISVVSSLGLLLLYYRKDIPRIYQVESLPNPKNVIKDPLIFKLAFPFMAIVVLAYFLTSFDLVPVSFVAVPAATILIVVARYNHRINTRSIIAEAPWQVVVFSLGMYIVVYGFGDNGLTSVLVHVTKSILLLGNPLSTVLAGLLFGAMAGSMNNLPSVMMGALTISHLNQNSFLIYANVIGNDIGPKFTPIGSLATLLWIYTLQRKHGVQITPIYYMKVGFIIAFPVLVLTLLSLYATIYFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C18</accession>
    <name>Q97C18_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iron binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0299008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C18"/>
    <dbReference type="STRING" id="273116.gene:9381061"/>
    <dbReference type="PaxDb" id="273116-14324501"/>
    <dbReference type="KEGG" id="tvo:TVG0299008"/>
    <dbReference type="eggNOG" id="arCOG04233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_2_8_2"/>
    <dbReference type="PhylomeDB" id="Q97C18"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01143">
        <property type="entry name" value="YvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="55"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="324" mass="35890" checksum="D0C04479EA26F106" modified="2001-10-01" version="1">MINMKGATKIAVAVIIVIAIVATSAYYYEHGIGKGNQAAIYLPGQKLPFTHPLTRIVSLLPSITTNLYALGAEKDIVGISTYTTYPKNISLKIPIVGQLDSVNYEELLNVSPQAVITIPSYYPQNVINKIVDMGIPVIEVGQNNFTQIEQQIFELGNITGTEGNASAIVNWMANSLAYLHTDANMLSNGTEKTVFYLLSTEGGYWTAGNETFINDFFRIADLRNIANETGYYTIPSSLIVKEDPEVVILDQYVNVDVMNSPPMNQVAAYKDMAYYTIFDDNYFNQPDFRSVYAVQWLIDEVYNTTVNIPAFPIPLSDNPAPSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C29</accession>
    <name>Q97C29_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TIGR01897 family CRISPR-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0288250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C29"/>
    <dbReference type="PaxDb" id="273116-14324490"/>
    <dbReference type="KEGG" id="tvo:TVG0288250"/>
    <dbReference type="eggNOG" id="arCOG03433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_695624_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97C29"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09660">
        <property type="entry name" value="Csx1_III-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10640">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3740.10">
        <property type="entry name" value="SSO1389-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027419">
        <property type="entry name" value="CRISPR-assoc_Csx1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013383">
        <property type="entry name" value="CRISPR-assoc_prot_DxTHG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052875">
        <property type="entry name" value="CRISPR_assoc_ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010171">
        <property type="entry name" value="CRISPR_Csx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019016">
        <property type="entry name" value="Csx1-like_HEPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053857">
        <property type="entry name" value="Csx1_CARF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01897">
        <property type="entry name" value="cas_MJ1666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02549">
        <property type="entry name" value="CRISPR_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169:SF1">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22230">
        <property type="entry name" value="Csx1_CARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09455">
        <property type="entry name" value="Csx1_HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160980">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR system endoribonuclease Csx1 CARF" evidence="2">
        <location>
            <begin position="6"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="CRISPR system endoribonuclease Csx1-like HEPN" evidence="1">
        <location>
            <begin position="327"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="46703" checksum="55836FB207EC5744" modified="2001-10-01" version="1">MIFMKILFAPIGDPKNYDEVKYRIDGKDYPSNASFKVIKEALNMDETIVYAGLSLCTNSNYNDYVSCSNYISNLVKDKLQLNDESVIVAPNIYGDKFTQPDGKNTLYFDFIYYNTLKILENEKPDEIYIDITHGINYMPLLATDAIRLATYTYLLGRNSIDLAIFNSEPVIKGYSGPYNIANVYSERLYVRQALLSVLMPFLSNENKNNITNKVLKGINNCNSDLIYSNANALFSGIFPFVLLSRREIEQCIKSVERKIKILDYNNFGIEFNTSHKPVYKDTMPIELSYLHSLLYIIEKIIGNMPDSSCVKISDIKDLAKRFSTSETISKLVESEIDSIEKLQNISDKPQLLAVIKHSQSSPKSICQADKRNLLAHGGFEQNVVYLWRENGEICVNYCDCIENVRNHLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C32</accession>
    <name>OTCC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ornithine carbamoyltransferase, catabolic</fullName>
            <shortName evidence="2">OTCase</shortName>
            <ecNumber evidence="2">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">arcB</name>
        <name type="ordered locus">TV0273</name>
        <name type="ORF">TVG0283269</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>carbamoyl phosphate + L-ornithine = L-citrulline + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C32"/>
    <dbReference type="SMR" id="Q97C32"/>
    <dbReference type="STRING" id="273116.gene:9381046"/>
    <dbReference type="PaxDb" id="273116-14324487"/>
    <dbReference type="KEGG" id="tvo:TVG0283269"/>
    <dbReference type="eggNOG" id="arCOG00912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_2"/>
    <dbReference type="OrthoDB" id="4696at2157"/>
    <dbReference type="PhylomeDB" id="Q97C32"/>
    <dbReference type="UniPathway" id="UPA00254">
        <property type="reaction ID" value="UER00365"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000008">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0056">Arginine metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113080" description="Ornithine carbamoyltransferase, catabolic">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="50"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="128"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="227"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="263"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <sequence length="305" mass="34157" checksum="8A83160021A71D13" modified="2001-10-01" version="1">MAKRDILSVLDMKNDLDEIINLSIELKKNRYRSYESLRNKMLGLIFEKPSTRTRTSLEVAIDQLGGHAVYLNPSEMQLGRGETISDTGHVLSRFLDAIAYRAFDHKNVVELARSTSIPVINALDDVEHPLQIVADFMTVKEKKGKFSGLKFSYIGDGNNMANSLMLGAAILGVDIYVATPHGYEPKSEFVDKAKQVAKERGSKVIITNDAIEAAKDADVIYTDVWISMGEESKRGEKEKAFTKYQINSDLVSNAKKDYIFMHCLPAHRGLEVTDDVADSINSVIFDEAENRLHSEKGVLYKLLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C55</accession>
    <name>Q97C55_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0264527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916508.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C55"/>
    <dbReference type="STRING" id="273116.gene:9381022"/>
    <dbReference type="PaxDb" id="273116-14324464"/>
    <dbReference type="GeneID" id="1440766"/>
    <dbReference type="KEGG" id="tvo:TVG0264527"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_5_2"/>
    <dbReference type="OrthoDB" id="29061at2157"/>
    <dbReference type="PhylomeDB" id="Q97C55"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052952">
        <property type="entry name" value="MFS-Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23527">
        <property type="entry name" value="BLL3282 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23527:SF1">
        <property type="entry name" value="BLL3282 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="1"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="380" mass="41457" checksum="23AD8AF75234AE9A" modified="2001-10-01" version="1">MRKLFPLIIASSSFFLSYYVRLSWSILSVYMPFRPTIEEEGLAFSLFFVGYVIVQIPSGFVSDRFSGGCVIGIALIGVAISAILSSLSKNITDEYLSSILMGFTAGWVYPASINVMNHYYKDRRSVYIGYYSIAWPLAIVISGLVLPTIAVRIGWQWGYYTSAMAAVVVAIAAFKLRTEKAGYSIDFSLIKDRNVLLLSLGGLLFFLSYWSLTLYAYKYFVGMGIDPLISGFIFSAMAISGLFSTPVSGFLISRLGLKPSVIISLIIYGLPIMSFAEVRSAAVLILISLLMGFFRFIITPGNSDLAIEIGRERAGSVSGIANMFWQSSGIIGPLVSSLIITSVGFNVLWIILAAIVISSSVLYYMIGSSLHAAAHHGDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C58</accession>
    <name>Q97C58_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0260458</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916505.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C58"/>
    <dbReference type="STRING" id="273116.gene:9381018"/>
    <dbReference type="PaxDb" id="273116-14324461"/>
    <dbReference type="DNASU" id="1440763"/>
    <dbReference type="GeneID" id="1440763"/>
    <dbReference type="KEGG" id="tvo:TVG0260458"/>
    <dbReference type="eggNOG" id="arCOG02702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049344_3_3_2"/>
    <dbReference type="OrthoDB" id="147504at2157"/>
    <dbReference type="PhylomeDB" id="Q97C58"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="44"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="252" mass="28655" checksum="BD4F885EBE49AF2B" modified="2001-10-01" version="1">MKSSDRLFTGRSENYSKFRPSYPVGIVKMLTDKYGLKKEMVIADIGCGTGILARMFLENGNKVYCIDPNGEMLKFAKDELSSFGNAIIVRGSAENTGLEDHSVNVISAGQSFHWFDTDKAKVEFRRILTAPYMVVLIWNDRDIDDGFTAEYEKIVSAYGKNYQGTGSTGLPPDTIPQFFNWAYDYYQYQNYQELDLEGLKGRYLSTFYSITQEYPKFQAAMKALETAFNAFSVDGKVRIRYITKVFVGKLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C97</accession>
    <name>Q97C97_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transport system permease protein P1P2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0212495</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916463.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C97"/>
    <dbReference type="STRING" id="273116.gene:9380976"/>
    <dbReference type="PaxDb" id="273116-14324420"/>
    <dbReference type="GeneID" id="1440723"/>
    <dbReference type="KEGG" id="tvo:TVG0212495"/>
    <dbReference type="eggNOG" id="arCOG00159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_2"/>
    <dbReference type="OrthoDB" id="57451at2157"/>
    <dbReference type="PhylomeDB" id="Q97C97"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050901">
        <property type="entry name" value="BP-dep_ABC_trans_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243:SF18">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER PERMEASE PROTEIN YCJP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243">
        <property type="entry name" value="MALTOSE TRANSPORT SYSTEM PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="63"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="268" mass="30419" checksum="A82ADEEA8B90AFEE" modified="2001-10-01" version="1">MDIKKFVIYIFLSLLGVLFLIPFIWLINEAFSPLNDIGLKFPSYYTLVPFYNVMTNKIFVNSIYEGLIQSFGATFLSVLFSVGPAYIFSRKRFRFRIPLLLGILLLTGFPVFSLLIPEYVFYVKTSLYNNLIGVILFLGVLNVPIDIWILKNSFDQIPSAIENASYIDGASSFKSMLYIFLPLATPVLAVLIILDFVINWGNFYVPYILLSSARLFPVSVEIYSFFGAYNVKYNELAAFSIIYTVPAVILYIFAQKYMIKALSGGIKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CB5</accession>
    <name>CDPAS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CDP-archaeol synthase</fullName>
            <ecNumber evidence="1">2.7.7.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carS</name>
        <name type="ordered locus">TV0187</name>
        <name type="ORF">TVG0192666</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + CTP + H(+) = CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="EC" id="2.7.7.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-archaeol synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.67" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760293.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CB5"/>
    <dbReference type="SMR" id="Q97CB5"/>
    <dbReference type="STRING" id="273116.gene:9380957"/>
    <dbReference type="PaxDb" id="273116-14324401"/>
    <dbReference type="GeneID" id="1441672"/>
    <dbReference type="KEGG" id="tvo:TVG0192666"/>
    <dbReference type="eggNOG" id="arCOG04106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105710_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CB5"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043338">
        <property type="term" value="F:CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01117">
        <property type="entry name" value="CDP_archaeol_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032690">
        <property type="entry name" value="CarS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002726">
        <property type="entry name" value="CarS_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003114">
        <property type="entry name" value="PRK04032.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650:SF1">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01864">
        <property type="entry name" value="CarS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000094183" description="CDP-archaeol synthase">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01117"/>
        </source>
    </evidence>
    <sequence length="183" mass="20032" checksum="6457D989928291C0" modified="2001-10-01" version="1">MNYPLLMAQAIILFVPALIANSGAVLTGGYFVLDRGKNFIDGRRILGDGKTLSGYLGGSFIGIVSGVIIYWISYATHFILGNYGSIALSIEIPAVMAFGSLTGDILGSFVKRRIGIKSGGKGGLLDQWPFALVAFLFLFLMERPFFLHYYFFAGIIVILAIVPPLHRAVNIIGYKLHKKDVPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CC3</accession>
    <name>Q97CC3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger ZPR1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0185868</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916434.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CC3"/>
    <dbReference type="STRING" id="273116.gene:9380949"/>
    <dbReference type="PaxDb" id="273116-14324393"/>
    <dbReference type="GeneID" id="1441664"/>
    <dbReference type="KEGG" id="tvo:TVG0185868"/>
    <dbReference type="eggNOG" id="arCOG04265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107446_0_0_2"/>
    <dbReference type="OrthoDB" id="14924at2157"/>
    <dbReference type="PhylomeDB" id="Q97CC3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="entry name" value="ZPR1, zinc finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004470">
        <property type="entry name" value="ZPR1-like_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00340">
        <property type="entry name" value="zpr1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="Zn_ribbon_ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="5">
        <location>
            <begin position="11"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="172" mass="19854" checksum="EB7081E2C848FE69" modified="2001-10-01" version="1">MDLPQEIETNMECPVCGSLLYLLTYDTDIPYEGRISIYTYQCKKCMYRKTEVYQEEEKNPVRITLKIESPEDLKTLVYRSRKADIYIPEIEASIDSAEYANGEITTVEGIIYRIGEKLDLLSYDDEEKEAVKEVRKKIDDIINGYFGSFTLIITDESGKSVIESEKAITEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CD0</accession>
    <name>Q97CD0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoribosylglycinamide formyltransferase 1</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">GAR transformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0180390</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6R)-10-formyltetrahydrofolate = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6S)-5,6,7,8-tetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GART family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916427.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CD0"/>
    <dbReference type="STRING" id="273116.gene:9380942"/>
    <dbReference type="PaxDb" id="273116-14324386"/>
    <dbReference type="GeneID" id="1441657"/>
    <dbReference type="KEGG" id="tvo:TVG0180390"/>
    <dbReference type="eggNOG" id="arCOG02825">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_1_3_2"/>
    <dbReference type="OrthoDB" id="27277at2157"/>
    <dbReference type="PhylomeDB" id="Q97CD0"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="9">
        <location>
            <begin position="3"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="200" mass="22448" checksum="F0A5AB5C56082EAF" modified="2001-10-01" version="1">MTKICVMVSGNGTTLQAIIDAVKNKKIDVEISKVIADRECLAIKRAEDNNIPYRILKRGEYFQRDLKEEMRSSKCDFFVLAGFLSIIGKEITDEFRYRIINTHPSLLPCFGGHGFYGRKVHEAVIKSGMKYSGCTVHFVTDEVDGGPIILQRCVSVEDVDDAQSLEEKIHGIEHSAIVEAISLLSNGHYKIEGKRVILNM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-16" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CE6</accession>
    <name>SYH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Histidine--tRNA ligase</fullName>
            <ecNumber>6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Histidyl-tRNA synthetase</fullName>
            <shortName>HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hisS</name>
        <name type="ordered locus">TV0155</name>
        <name type="ORF">TVG0164058</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916410.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CE6"/>
    <dbReference type="SMR" id="Q97CE6"/>
    <dbReference type="STRING" id="273116.gene:9380925"/>
    <dbReference type="PaxDb" id="273116-14324369"/>
    <dbReference type="GeneID" id="1441640"/>
    <dbReference type="KEGG" id="tvo:TVG0164058"/>
    <dbReference type="eggNOG" id="arCOG00404">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_3_1_2"/>
    <dbReference type="OrthoDB" id="8659at2157"/>
    <dbReference type="PhylomeDB" id="Q97CE6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707:SF1">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136329" description="Histidine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="426" mass="47612" checksum="9822A959A0C5FB0B" modified="2001-10-01" version="1">MQIERIKGFRDFYPEDMEIEKFIFETASSVAESYGFKRIDFPSLEYIDLYRIKSGEELLNQTYSFTDRGGREVTLIPEATPSTVRMLTSRKDIAKPVRWYSFPKVWRYEEPQAGRFREHYQFNADIFGPSNEEADAEIISLASGILDGLGLSGAYEIRVNSRIMMEDILNGMGIADPYTVFSIVDRFHKVSKETFVDDLMSVGLSEENSNTIYRMCSEASEPGGISGLFGSGKVSKAAERLIRTIDILKEYGVKSVKYDFSIVRGLSYYTGLVFEAYDKSGQFRAILGGGRYDNLAKLFSEQDIPAVGFGMGDAVISLLLKSKGVKAPHAKKSVYVCRVGTVKAERIASISKMLRSSGFIVSAEIMDRSLSSQLKYASYEGCEYAVIAGERDLENNSVTVRDLSTGEQEIVTLGDLVPFLEKSTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CF5</accession>
    <name>Q97CF5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0152070 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0152070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CF5"/>
    <dbReference type="PaxDb" id="273116-14324360"/>
    <dbReference type="KEGG" id="tvo:TVG0152070"/>
    <dbReference type="HOGENOM" id="CLU_1472163_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="183" mass="22314" checksum="EEC91AA8E192D69A" modified="2001-10-01" version="1">MIACDTPPYKMQDFINYVRTLPKNKYYDIYFPLLFIDTELDRIQESVYGMEDWIYSFSKDLKWVPRRTDCPATPLYYKKIVVSTPYMVHLNRLFDKEKMILKQASWEWMKPEIRKKYGSIENFIKERRNDFKTDFGEHNTLYYDESKFCKLPRVLERFRGMKSEDIIKQKTYEINNGIFPDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CG7</accession>
    <name>Q97CG7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0142586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CG7"/>
    <dbReference type="STRING" id="273116.gene:9380903"/>
    <dbReference type="PaxDb" id="273116-14324348"/>
    <dbReference type="KEGG" id="tvo:TVG0142586"/>
    <dbReference type="eggNOG" id="arCOG01285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_2_2_2"/>
    <dbReference type="PhylomeDB" id="Q97CG7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="53"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15295" checksum="834632CCEDA90323" modified="2001-10-01" version="1">MKMGQLSRFWRESEHRYRLLGSKCGNCGTVYFPPREVCPTCHRDSIGKMKDIELSGDGVIESFTVVHEAPPRFSRQRPYILALIKTEEGPMLTGQIVDADPDEVEIGKKVHAVFRRMGEDGESGVIVYGYKFVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CH0</accession>
    <name>Q97CH0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0139918 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0139918</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916385.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CH0"/>
    <dbReference type="STRING" id="273116.gene:9380900"/>
    <dbReference type="PaxDb" id="273116-14324345"/>
    <dbReference type="GeneID" id="1441615"/>
    <dbReference type="KEGG" id="tvo:TVG0139918"/>
    <dbReference type="eggNOG" id="arCOG05389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2447772_0_0_2"/>
    <dbReference type="OrthoDB" id="55568at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="89" mass="10269" checksum="6273D027E303A9B9" modified="2001-10-01" version="1">MKEYETETKGGKVEARVFIDSGKFVIYGYEEDGKMEKKYRLVLKGEKENRSFFIIPTGNKRNLAIDADFESEVYVLKDGKSVKVSDLFD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-16" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CI4</accession>
    <name>CCA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CCA-adding enzyme</fullName>
            <ecNumber evidence="1">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cca</name>
        <name type="ordered locus">TV0117</name>
        <name type="ORF">TVG0124714</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a tRNA with a 3' CCA end + 2 CTP + ATP = a tRNA with a 3' CCACCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:76235"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="Rhea" id="RHEA-COMP:18655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="ChEBI" id="CHEBI:195187"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:76236"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916373.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CI4"/>
    <dbReference type="SMR" id="Q97CI4"/>
    <dbReference type="STRING" id="273116.gene:9380885"/>
    <dbReference type="PaxDb" id="273116-14324331"/>
    <dbReference type="GeneID" id="1441603"/>
    <dbReference type="KEGG" id="tvo:TVG0124714"/>
    <dbReference type="eggNOG" id="arCOG04249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044679_1_0_2"/>
    <dbReference type="OrthoDB" id="7378at2157"/>
    <dbReference type="PhylomeDB" id="Q97CI4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160016">
        <property type="term" value="F:CCACCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1550">
        <property type="entry name" value="Archaeal tRNA CCA-adding enzyme catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.30">
        <property type="entry name" value="CCA tRNA nucleotidyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01264">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048833">
        <property type="entry name" value="CAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008229">
        <property type="entry name" value="CCA-adding_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042090">
        <property type="entry name" value="CCA_tRNA_nucleotrans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015329">
        <property type="entry name" value="tRNA_NucTransf2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03671">
        <property type="entry name" value="cca_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643:SF1">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21133">
        <property type="entry name" value="CAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09249">
        <property type="entry name" value="tRNA_NucTransf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005335">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0692">RNA repair</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000139087" description="CCA-adding enzyme">
        <location>
            <begin position="1"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01264"/>
        </source>
    </evidence>
    <sequence length="437" mass="50283" checksum="F987964456A921D4" modified="2001-10-01" version="1">MIDYSAILNRYRPDEQELQKLKSISEYLTTRASEICRHKNIRADPVLVGSYAKGTNLKDGDLDLFIAFDRDYEEKIMEKLGLSIGHDLLPNGKEKYAEHPYVSGEIDGIKIDVVPCFKMEFGQKKISAVDRTLLHTRYVNEKLDGASKDQVRLLKVFMKSVGVYGAEARTYGFSGYLCELLIIRYGNFENVLRLFSTTRGRLKIDDDERFEDPMVLIDPVDPDRNVASAVSLESLSRMRIASKMFLDNPSESFFDLERKPIKPKYHDRGTCIRIYSIEKPDLTDDVLYPQIYKFKLALLRLMEEYNMEPVGSAIDVADRIYVLIETRKFISDSIRIHVGPPADTSNSIDFIKKWLTRERSRGPYLVGNRLYVDTVEERKTPEEIVLSNISKYSIGKNLDKLKNTIKVQKFEEIKGRIRVLDRFFSEEAFGGLNSLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CJ5</accession>
    <name>Q97CJ5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR system ring nuclease SSO2081-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0111880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760292.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CJ5"/>
    <dbReference type="STRING" id="273116.gene:9380874"/>
    <dbReference type="PaxDb" id="273116-14324320"/>
    <dbReference type="GeneID" id="1441593"/>
    <dbReference type="KEGG" id="tvo:TVG0111880"/>
    <dbReference type="eggNOG" id="arCOG03847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136111_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09723">
        <property type="entry name" value="Csx1_III-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10770">
        <property type="entry name" value="Hypothetical protein VC1899 like domain (Restriction endonuclease-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019848">
        <property type="entry name" value="CRISPR-assoc_prot_Csx14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019092">
        <property type="entry name" value="SSO2081-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03642">
        <property type="entry name" value="cas_csx14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09623">
        <property type="entry name" value="Cas_NE0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR system ring nuclease SSO2081-like" evidence="1">
        <location>
            <begin position="12"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="244" mass="28416" checksum="0041E2B00F382A4B" modified="2001-10-01" version="1">MKTLLINPMGTSPMVATEMYEYLKDEISDVILIYTDNKYVKAGTLAAKASLEDKFHAHVHLQKLDFDDIRSDNDMIKFIKVISETVKKEKYQFGTEKIIVNASGGRKIETIILSVYASIFSFDEVYNIINKSVTSYNEEYEKIKDKISEFDDENYKDKYKEYKDLLDHVFYPDMNDLYFLKVPVVKLPKDEINNIKAALTSSSREYSDLADYKLKSYKDSGFITYDNSRIYRTELGDIILSYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CM3</accession>
    <name>Q97CM3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tropomyosin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0079184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CM3"/>
    <dbReference type="STRING" id="273116.gene:9380844"/>
    <dbReference type="PaxDb" id="273116-14324292"/>
    <dbReference type="KEGG" id="tvo:TVG0079184"/>
    <dbReference type="eggNOG" id="arCOG01159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063607_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CM3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055545">
        <property type="entry name" value="DUF7121"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23435">
        <property type="entry name" value="DUF7121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="125"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="297" mass="35086" checksum="189D3A7210A75AA7" modified="2001-10-01" version="1">MTMTDLLQEFEEKRDLLKKAVEEHIKKRDEAAQQSRSYAEQRDQLNAKVHEMREQVKEKISQKNALIEEVQKVRAEKEEHFKKLSDLRKDYKKLSEESNYTNISQREIKAKEKELQKLITKQQTMQLTKAEEDKIVSEIKKLNNEIKKMKEDRTKQLNENEKVKELLAEIDKEKTIARDLKKKAEEISNKISEISNDINKSLQDLDEVRKKADEMHEMAVKYGKESEQEHEAFIQAKNDLRDLEKVIAGLRTKKRATKKKEKDDELQQKAEVLFEKFKNGEELTTDDLLILQKAGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CN0</accession>
    <name>Q97CN0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1611 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0072555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916328.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CN0"/>
    <dbReference type="STRING" id="273116.gene:9380837"/>
    <dbReference type="PaxDb" id="273116-14324285"/>
    <dbReference type="GeneID" id="1441558"/>
    <dbReference type="KEGG" id="tvo:TVG0072555"/>
    <dbReference type="eggNOG" id="arCOG02828">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059741_0_1_2"/>
    <dbReference type="OrthoDB" id="30617at2157"/>
    <dbReference type="PhylomeDB" id="Q97CN0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011669">
        <property type="entry name" value="DgcN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035086">
        <property type="entry name" value="DgcN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035402">
        <property type="entry name" value="DgcN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40690:SF1">
        <property type="entry name" value="DUF1611 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40690">
        <property type="entry name" value="GLL3100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07755">
        <property type="entry name" value="DUF1611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17396">
        <property type="entry name" value="DUF1611_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026760">
        <property type="entry name" value="UCP026760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-glutamate N-acetyltransferase-like N-terminal" evidence="2">
        <location>
            <begin position="39"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="D-glutamate N-acetyltransferase-like C-terminal" evidence="1">
        <location>
            <begin position="140"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="38148" checksum="DE5F7BB13A5EF368" modified="2001-10-01" version="1">MENATILSEGVFATTYGKTANGLVRYSKRYHITSVIDSRLAGQDAGQVLMHKPSGIPIISSVEEAINGHAETLIVGIASDGGVLPENYRPYIRTALGYGMNIVSGLHDFVSDDPEFVKIARRTGSRITDVRKLFKDKKYFFTGNIEKVKAKKIAVLGTDSAIGKRTTAIYLNRAMKAIGKKSVMIGTGQTSWMQGFKYTVVVDAIVNDFVAGALETITYEAWDSERPDYMFIEGQGSVLHPAYPGSFEIIGATRPDAIILQHAPMRKYFDGFPGYEIPPLEKYIKILELLSNRKVIAIALNTENMDESMVKEEKSRLQAKFGIPVFDPLQRLSTVTREIDSYNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-20" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CP9</accession>
    <name>AATB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpB</name>
        <name type="ordered locus">TV0052</name>
        <name type="ORF">TVG0055637</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The B chain is a regulatory subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916309.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CP9"/>
    <dbReference type="SMR" id="Q97CP9"/>
    <dbReference type="STRING" id="273116.gene:9380817"/>
    <dbReference type="PaxDb" id="273116-14324266"/>
    <dbReference type="GeneID" id="1441539"/>
    <dbReference type="KEGG" id="tvo:TVG0055637"/>
    <dbReference type="eggNOG" id="arCOG00865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022916_0_0_2"/>
    <dbReference type="OrthoDB" id="32941at2157"/>
    <dbReference type="PhylomeDB" id="Q97CP9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144671" description="A-type ATP synthase subunit B">
        <location>
            <begin position="1"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00310"/>
        </source>
    </evidence>
    <sequence length="460" mass="51211" checksum="5D06DBC23A20C1C4" modified="2001-10-01" version="1">MPKLTYKSVSEISGPLLFVENVPNAAYNEMVDIELDNGETRQGQVLDTRKGLAIVQIFGATTGIGTEGTRVKFRGETARLPISEDMLGRVFNGIGEPIDGGPEILAKERMEITSNAINPYSREEPSEFIETGISAIDGMNTLVRGQKLPIFSGSGLPHNQLAAQIARQAKVLDSSENFAVVFGAMGITSEEANYFTNQFRETGALSRSVMFLNLSSDPSMERIILPRIALTTAEYLAFQKEMHILVILTDMTNYCEALREISSAREEVPGRRGYPGYMYTDLSTIYERAGKLKGNNGSITQIPILTMPGDDITHPVPDLTGYITEGQVVVSRDLNRKGIYPGIDVLLSLSRLMNQGIGKGHTREDHRGLADQLYSAYASGKDLRSLTAIVGEEALSQNDRKYLRFADTFEERYLKQDFFEDRSIEDTLNLGWELLADLPESDMKRVKPDHIKKYGKWKKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CQ2</accession>
    <name>AATC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpC</name>
        <name type="ordered locus">TV0049</name>
        <name type="ORF">TVG0051618</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916306.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CQ2"/>
    <dbReference type="SMR" id="Q97CQ2"/>
    <dbReference type="STRING" id="273116.gene:9380814"/>
    <dbReference type="PaxDb" id="273116-14324263"/>
    <dbReference type="GeneID" id="1441536"/>
    <dbReference type="KEGG" id="tvo:TVG0051618"/>
    <dbReference type="eggNOG" id="arCOG02459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777607_0_0_2"/>
    <dbReference type="OrthoDB" id="4272at2157"/>
    <dbReference type="PhylomeDB" id="Q97CQ2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="entry name" value="V-type ATP synthase subunit C domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00314">
        <property type="entry name" value="ATP_synth_C_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014272">
        <property type="entry name" value="ATPase_V0-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050873">
        <property type="entry name" value="V-ATPase_V0D/AC39_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002266">
        <property type="entry name" value="PRK01198.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38682">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38682:SF1">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000119374" description="A-type ATP synthase subunit C">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00314"/>
        </source>
    </evidence>
    <sequence length="356" mass="41174" checksum="2BAD975CB4EB6C1B" modified="2001-10-01" version="1">MDATYVGAYGRLKVYEVDFLKKDFLEHLISLDKPSDFSSVLYASVYKEDYDALTSIYREPDLTEMAINRHLVRMNRIAAFAIPPLAKNALTAYISKWDIENIKTVISSKFLGHGLKETEMFIVSFRDIPMGLIGGVLTNEDYRNMMNLPNVEAIINYLTRYGYGSYMLQFIEDYRKTGDISPMLYSLDRYYYARLLESLKYYNGDEGPVINYIRSDIDRINLNTILKGKKLNISYERFFSGLVDGGNIPINAIHDFYGNSDILSMIDSIKRYYDLEDAKNKYASDGNLYHFDVSMRNIMITKYMGTMSMLPLSLDSIFYFIIRAEIERQNLRTIYEAKLHGAPKESIYDLMINGVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CQ5</accession>
    <name>GLYA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="1">SHMT</shortName>
            <shortName evidence="1">Serine methylase</shortName>
            <ecNumber evidence="1">2.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyA</name>
        <name type="ordered locus">TV0046</name>
        <name type="ORF">TVG0048225</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible interconversion of serine and glycine with a modified folate serving as the one-carbon carrier. Also exhibits a pteridine-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916304.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CQ5"/>
    <dbReference type="SMR" id="Q97CQ5"/>
    <dbReference type="STRING" id="273116.gene:9380811"/>
    <dbReference type="PaxDb" id="273116-14324260"/>
    <dbReference type="GeneID" id="1441533"/>
    <dbReference type="KEGG" id="tvo:TVG0048225"/>
    <dbReference type="eggNOG" id="arCOG00070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_2"/>
    <dbReference type="OrthoDB" id="5821at2157"/>
    <dbReference type="PhylomeDB" id="Q97CQ5"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000114">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113728" description="Serine hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="119"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="1">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <sequence length="426" mass="46751" checksum="8635212A990251CE" modified="2001-10-01" version="1">MAYQDALSIRELAQQHNALFGDSVALIASENVMSPLAREVMISDLESRYAEGLPHHRYYQGNNFVDLIEDKTNELLSKLFKTEQTDPRPISGTNANSAAIYALAKPGDLVAVPALSGGGHISAAEFGILGMRGVRTISYPYDKNTMTVDPDEASKIIKREKPKVCLFGQSVFMFPVPLKEMKGAFDEVGCKVWYDGAHVLGLIAGRKFQDPLREGADIVTGSTHKTFPGPQHGVILGNTDSETWKSVRRAVFPGVLSNHHLNAMAALGITAAEELEFGEKYASDIIDNAKALAGELYSLGFKVLAEERGFTESHTMAVDVTQNGGGKYVAETLEASGIILNKNLLPWDDNKKSQNPSGIRIGVQEVTRTGMGKSEMKEIASLIYRAIVKKEEPSKIKEEVKDLKSSFRHVKYCYGDVDAYEYIKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CS4</accession>
    <name>Q97CS4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6 7">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsZ</name>
        <name evidence="10" type="ORF">TVG0027936</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CS4"/>
    <dbReference type="STRING" id="273116.gene:9380792"/>
    <dbReference type="PaxDb" id="273116-14324241"/>
    <dbReference type="KEGG" id="tvo:TVG0027936"/>
    <dbReference type="eggNOG" id="arCOG02201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_2"/>
    <dbReference type="PhylomeDB" id="Q97CS4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF3">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN FSZA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 7 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 7" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="8">
        <location>
            <begin position="101"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="9">
        <location>
            <begin position="294"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="109"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="196"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="434" mass="47091" checksum="1518265F92D76ABF" modified="2001-10-01" version="1">MRRQWSSFPLRLFRQTLLSTTHQYRINYEIKALSLQISKPDRCQQMYKYLNDVVRHMGDITDLLSKVSIDDISDDFWDDNPTPSIPEDEEIANIYKTLNVKIKVVGCGGGGSNTVSRLYEEGLKGADLIALNTDASHLKTIKVAKKLLIGYRTTRGLGTGADPKVGEEAAAEEIVSIKKMVQNTDIVFVTAGLGGGTGTGSAPVVAKAAKEAGAIVISVVTLPFDSEGPMRMDNAVIGLENLAQFSDTLIAIPNQRLLSEVPNAEMKTAFAYADRVLADTIRAIVEIITKTGVINIDYSDIKTVMKSGGVAMIGMGQSKKGGDRIMTALEEALKPRLIDVDISTAKDCIFKIIAPPDITVSEVGKAMSEIKKRITAKSRIIWGLTVDKNLDQDVKVLIFMTGVNSAYLVRDLESARRLANAFTGSTSTGIDIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CT7</accession>
    <name>Q97CT7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-terminal acetyltransferase complex subunit [ARD1]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0012647</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        <location>
            <begin position="21"/>
            <end position="453"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="EMBL" id="BAB59147.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="476" mass="52249" checksum="6103964B764AEDA2" modified="2001-10-01" version="1">MAKGIIEELDNSKINSFHLRTWLIAGMGFFTDAYDLFVIGTVISILPLAGWDKLNTYDISILSSISLLASVIGAVIFGRLLDALGRKRVYGFELMLLILGALGSAFVVPVNGINYLIAWRFILGLGIGGDYAGSSTIMTEFSNTKNRGQLVGMVFSLQGVGLIAGPLITLGLLSFIHNLDLVWRVLFFIGAVPAFIVLYGRRAIGETPRYALRVKGDASSAMQSVEKISGDSKVSVSREDLEMSNIKWTDLFKNRYFMLSLIGTAGTWFLLDWAFYGNSIMSHEMLSALIPSSMSGLNKLKLSTFYTLIIFAVSAFPAYWIATFTVDRIGRKRMQVIGFLIMGITFGILGIFRYLMSPTFVTYFLLVYGISYFFTEFGPNVTTFIYAPEMFPTAIRGLGSGISSAGGKTGAFIGTALNVIIFTLYGEGELFLILAGISMLGMLMTLVLLPETSRRKLEDISGEGRYIVRTASGFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q978B5</accession>
    <name>Q978B5_THEVO</name>
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        <submittedName>
            <fullName evidence="6">DNA adenine modification methylase</fullName>
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        <name evidence="6" type="ORF">TVG1555627</name>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family.</text>
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            <end position="423"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="703" mass="81115" checksum="B073C4532C7CFC05" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q978C1</accession>
    <name>Q978C1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1548516 protein</fullName>
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    <organism evidence="2 3">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="GeneID" id="30928351"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="74" mass="8100" checksum="4B758A74D0F1ADC3" modified="2001-10-01" version="1">MHTEKAGIRASFLSTFGIIFLTLGVVGLLGLFNVQILYSFGTLISIVSIIAGVYFILSAHISRKNRVKRSARQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q978C3</accession>
    <name>Q978C3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1547379 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1547379</name>
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    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
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            <begin position="36"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
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            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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                <person name="Aramaki H."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
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            <begin position="51"/>
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        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="EMBL" id="BAB60628.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
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    </evidence>
    <sequence length="179" mass="20842" checksum="3032670C52751B20" modified="2001-10-01" version="1">MSTKFSNLQIVKDHVRTLKLYNNGGVDIISVIINFILPFVFALTFLYFHNFLIPSIITVLITVLSIFISLLLNLLFLMYSILSRRLQSGVNAGSPQNDNERSVENNVRIRLLKETYYNVKFSVLLCIYALIAIILYVLLPKELYLDLFLGFIVYYLLFVFIVTLLMILKRTHTLFSREF</sequence>
</entry>
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                <person name="Yamamoto Y."/>
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                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
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                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_10_2_2"/>
    <dbReference type="OrthoDB" id="19089at2157"/>
    <dbReference type="PhylomeDB" id="Q978D7"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
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        <property type="entry name" value="ComplexI-like_HydComp"/>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
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            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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    <dbReference type="PaxDb" id="273116-14325697"/>
    <dbReference type="GeneID" id="1442147"/>
    <dbReference type="KEGG" id="tvo:TVG1507900"/>
    <dbReference type="eggNOG" id="arCOG02113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140783_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012440">
        <property type="entry name" value="DUF1641"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07849">
        <property type="entry name" value="DUF1641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="164" mass="18020" checksum="80E39D54586EFD01" modified="2001-10-01" version="1">MVEQKQEQQAQVPQEDQDDLEVLLNKVVENKDTIVSLMDLLEKMKEAGIIDVFMSIAKDYTPTDVEFLAKFFTERELTEALLKVGNSMMGLLYGLAGERTSDVIKAVSFNLQGVTDGLVDGIKNPQSFSALKLMALLKDPDISALITGLVNAAKVLVSSIKKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q978G2</accession>
    <name>Q978G2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1504499</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the archaeal flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917684.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978G2"/>
    <dbReference type="STRING" id="273116.gene:9382265"/>
    <dbReference type="PaxDb" id="273116-14325692"/>
    <dbReference type="GeneID" id="1442142"/>
    <dbReference type="KEGG" id="tvo:TVG1504499"/>
    <dbReference type="eggNOG" id="arCOG01829">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051124_0_1_2"/>
    <dbReference type="OrthoDB" id="102632at2157"/>
    <dbReference type="PhylomeDB" id="Q978G2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006325">
        <property type="entry name" value="PRK08541.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903:SF1">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="242" mass="25165" checksum="82DAB1A7005334AB" modified="2001-10-01" version="1">MYIVKKMPILKLLNSIKRIFKTDDSKAESGIGVLIVFIAMILVAAVAASVLIHTAGILQQKASSTGTTTIQQVSTGVIINQIIGYDNASPPESGGISYLAIFVSISSGGTPVDLANTTVTLTVGGVTAVLVYNSSVFADIAGSGSANLFSLPVWSELSAKNRNPTSFGVIVNFDPSHSLTRHYPVLSVGDSAAIIINVTNTFGSNITQDSVVSGQVTPETGTPAIIQFTAPTSFTTKSIEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q978H7</accession>
    <name>Q978H7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Type II methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N-4 cytosine-specific methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1487993</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="8"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917671.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978H7"/>
    <dbReference type="STRING" id="273116.gene:9382249"/>
    <dbReference type="REBASE" id="685109">
        <property type="entry name" value="M.TvoV"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325677"/>
    <dbReference type="DNASU" id="1442129"/>
    <dbReference type="GeneID" id="1442129"/>
    <dbReference type="KEGG" id="tvo:TVG1487993"/>
    <dbReference type="eggNOG" id="arCOG00115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060286_0_0_2"/>
    <dbReference type="OrthoDB" id="56674at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0680">Restriction system</keyword>
    <keyword evidence="4 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="9">
        <location>
            <begin position="20"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="9">
        <location>
            <begin position="173"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="316" mass="36822" checksum="0CC2697EB8A2C863" modified="2001-10-01" version="1">MDYLDIGQAADNKYYSSKRTLPLAIMKENAKEYQPLKINTIKTCSCKENNLNCLTAKEWIKRQIGVWEFYYEKRDIRDKTVHPATFPISLAKQVIELFSHEGELVVDPFVGSGTTLVAARDSNRNAVGFDLQEKYIKLCAERLSTQRIVGNAQQVAIEEDARNISNYFNEESISLIFTSPPYANLLNRERKNKSRRNRKNNQLGKVEQYSQDPRDLGTLDVESYTKEMGDIFEKVKPALRTKGHCVINVPDMWWENKRIAIHVYLINEMTKRGYELRNIIIWDKRNLVNQIGIFGWPSNYITMGVTFEYLLDFWKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q978I1</accession>
    <name>Q978I1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1481172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978I1"/>
    <dbReference type="STRING" id="273116.gene:9382245"/>
    <dbReference type="PaxDb" id="273116-14325673"/>
    <dbReference type="DNASU" id="1442124"/>
    <dbReference type="KEGG" id="tvo:TVG1481172"/>
    <dbReference type="eggNOG" id="arCOG01687">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1335091_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="215" mass="24802" checksum="F013E906A6CC0628" modified="2001-10-01" version="1">MTFMPEKRNADLLFRGFSKTRLMVITLIAVNGPMTVTQLSKVIKTTRSNLYQAIKEMVSDGILVQTRTVSEKNYVEKFYDINTDLFDAIKYGDIRKAIEEMDEDKLRNILYSFLRSASAILSIMAEQVNLSNKDDLDKYVDYVRKNLLIISFSTHSTKDMSKFSSMLSNFFTEIDDDNDEKKDEHLLYIVGFPILNQMGFISKSRHEKLTGDGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q978I2</accession>
    <name>Q978I2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1479283</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917665.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978I2"/>
    <dbReference type="STRING" id="273116.gene:9382244"/>
    <dbReference type="PaxDb" id="273116-14325672"/>
    <dbReference type="GeneID" id="1442123"/>
    <dbReference type="KEGG" id="tvo:TVG1479283"/>
    <dbReference type="eggNOG" id="arCOG00135">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_751470_0_0_2"/>
    <dbReference type="OrthoDB" id="57098at2157"/>
    <dbReference type="PhylomeDB" id="Q978I2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06173">
        <property type="entry name" value="MFS_MefA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23513">
        <property type="entry name" value="INTEGRAL MEMBRANE EFFLUX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23513:SF6">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="219"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="399" mass="43556" checksum="717AE314E459656A" modified="2001-10-01" version="1">MYNLKSNNSGNKNFKFLVSSVWFSHTAFSAYNLIVIWVVLFITKKPLLAGFSDSLLTLPLMISVLVGAYVDKSKRKKTLAFISSLFRVVLVIIIMISIQFWPSIDVIITIYAATFLIGFTSDVVDSVRATWTKAFLGDKHYKKGSSEMSSVIMASQGVGFIISGILIALGFEASFLIIGLFFALSSVPLIMIKYTAYGTSKSIHTGVAEALTLLRKDKRLAEIIAINFVSNFSIGVAAILYISLMQEGYRLPAMYVSLIFGVIVLGMVFGSISASKISGKVGRINLLVFIVLGVSFVSISLIHSVYVLVLPSFLAGLSIGIESSVLSTLSMRIVPHEMMARIQGSMNTFGMAASALSSLIGGLLYQLFGPSYSFLMVGLIIISADFVFLAFRSLYYLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q978I3</accession>
    <name>Q978I3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose 1-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1479238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917664.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978I3"/>
    <dbReference type="STRING" id="273116.gene:9382243"/>
    <dbReference type="PaxDb" id="273116-14325671"/>
    <dbReference type="GeneID" id="1442122"/>
    <dbReference type="KEGG" id="tvo:TVG1479238"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="PhylomeDB" id="Q978I3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004746">
        <property type="entry name" value="PRK06077.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="247" mass="26786" checksum="AB723E23F6DC2B07" modified="2001-10-01" version="1">MDLKDKVAVVTGSGRGIGRAISVALAKNRCKVIINVKKRLEEGQETLRMVEEYSQGILVKADVSTREGCASIINEARKNFDSIDILVNNAGLGIAMPFMNSDDSLIQKLIDTNIMSVIRCSQEIGRVMEDGGTIINVSSVAGLKPMPLLPIYGMTKAAIINLTQYMAYDFAQRRIRVNAVAPSVVSTKMGNSLVDISGLKEDKFAKEYTLTGKIIDPDEVADAVIFLAKSDSITGQTLVIDSGWLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q978I8</accession>
    <name>Q978I8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1473478</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978I8"/>
    <dbReference type="STRING" id="273116.gene:9382236"/>
    <dbReference type="PaxDb" id="273116-14325666"/>
    <dbReference type="KEGG" id="tvo:TVG1473478"/>
    <dbReference type="eggNOG" id="arCOG03688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1763960_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978I8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052707">
        <property type="entry name" value="OsmC_Ohr_Peroxiredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42830:SF2">
        <property type="entry name" value="OSMC_OHR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42830">
        <property type="entry name" value="OSMOTICALLY INDUCIBLE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="OsmC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="147" mass="16973" checksum="50369AB7BF8BBB6F" modified="2001-10-01" version="1">MKCLYVMDTYTYKADVEWIGARRTILKTDAGDLEVSSPPEFGGPEGKFCPETLFPGILASCLLTTFLEFRERMGIDLKEWKSEATAELGPSPEKGFRFQRIRIHVKLRVSEQDKEKIPRAMELAHKYCFISRAIKNNVEEIVDYEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q978K2</accession>
    <name>Q978K2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1460734</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917645.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978K2"/>
    <dbReference type="STRING" id="273116.gene:9382222"/>
    <dbReference type="PaxDb" id="273116-14325652"/>
    <dbReference type="GeneID" id="1442103"/>
    <dbReference type="KEGG" id="tvo:TVG1460734"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076812_1_0_2"/>
    <dbReference type="OrthoDB" id="85195at2157"/>
    <dbReference type="PhylomeDB" id="Q978K2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="227"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="278" mass="31580" checksum="059801B3E3C44EFD" modified="2001-10-01" version="1">MKVSEIMTPNPITYRVPSSINEVIKVLIKYNVTGIPITDTAGHYVGFVSRRDIFSNPRETQTAMVMRRSNAVYEDDEVKVAAVEMLNQRKRHLTVINREGIVTGILTPQNFMKVIRDTYGTVKVKEVMKGVSVPIWYKTPLPAILTIMNVSNIFSFPVINDEGGFIGLLVDRDIFDRIDLKFDSVLSEMGIADDEDPWSWTGLRNVFKYVVERADIKLPKISAEEIMVKDPTVVYANDRLSTAADLMIKYNYNQLPVLDDTHGIYGMLYDIEMLGVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q978K7</accession>
    <name>Q978K7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Electron transfer flavoprotein alpha-subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1454962</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917640.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978K7"/>
    <dbReference type="STRING" id="273116.gene:9382217"/>
    <dbReference type="PaxDb" id="273116-14325647"/>
    <dbReference type="DNASU" id="1442098"/>
    <dbReference type="GeneID" id="1442098"/>
    <dbReference type="KEGG" id="tvo:TVG1454962"/>
    <dbReference type="eggNOG" id="arCOG00447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_1_2"/>
    <dbReference type="OrthoDB" id="307696at2157"/>
    <dbReference type="PhylomeDB" id="Q978K7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000004">
        <property type="entry name" value="Electron transfer flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF11">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN, SUBUNIT ALPHA (ETFA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="293" mass="31524" checksum="FA97D35A3C0379FC" modified="2001-10-01" version="1">MKALVVSDDINFLKQACTLVNGKAEYDAVYVGEGRVSGVGASTLYTVKKGTPYDAVADGILEIMGNYDIIVAGSTEFGREVAGYIAGKTGYYAATEIFSLEFSNGKAKTKRFFYGGKTVIEEESDAKVFTVAPGVIEAKEFESKPEEKDLPIKPSKVRVNKVVPKNAGGVRLEDAKIIVSVGRGIGSKENISKIEPLVNAVKGELAGSRPVCMDYGWLSEDRQVGLSGKKVSPKLYIALGISGQIQHIAGMRNSKTVIAINKDKNAPIFQECDYGIVGDIFAIVPKIVEELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q978N0</accession>
    <name>Q978N0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase M20 domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1433041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978N0"/>
    <dbReference type="STRING" id="273116.gene:9382193"/>
    <dbReference type="PaxDb" id="273116-14325624"/>
    <dbReference type="DNASU" id="1442076"/>
    <dbReference type="KEGG" id="tvo:TVG1433041"/>
    <dbReference type="eggNOG" id="arCOG01108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031812_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q978N0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071713">
        <property type="term" value="F:para-aminobenzoyl-glutamate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046657">
        <property type="term" value="P:folic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052030">
        <property type="entry name" value="Peptidase_M20/M20A_hydrolases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017144">
        <property type="entry name" value="Xaa-Arg_dipeptidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30575">
        <property type="entry name" value="PEPTIDASE M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30575:SF0">
        <property type="entry name" value="XAA-ARG DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037226">
        <property type="entry name" value="Amidohydrolase_ACY1L2_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="2">
        <location>
            <begin position="159"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="381" mass="42168" checksum="EF195E6B2C0FA949" modified="2001-10-01" version="1">MAMTTEFANEITDVAKAIYEYAELGSSEYKSSSYLVEAFKRHGFQVDFPYLGMKTAFKARYFNGKPMVGLLAEYDALPNGHSCGHDLIAAWAFGTAIRLKDEFGISVTVFGTPSEEGIGEYAGSKAKFAQSGAFSDVDYVIGMHPDDRWAVGSTSLADLTIEAQFIGKSSHLLSPDYGINALDPLVESYVALSNFRRSVPNSKYPVIGMIVKEGGRASNVIPDKASMEIDIRAKKKADLAGFVDQIERILKMEGEMHYCKLNINEVTPIYDEYKSNRVIDSLLEEECQKHAVNPFNVDRSGEEPMGSTDEANVSQVIPTGHLDVKIVDPGIPAHTDEFRIAADPSRSGKTLLKAIDITVETIKTIWQDKKFMNKIKEEFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q978P8</accession>
    <name>Q978P8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1415417</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978P8"/>
    <dbReference type="STRING" id="273116.gene:9382175"/>
    <dbReference type="PaxDb" id="273116-14325606"/>
    <dbReference type="KEGG" id="tvo:TVG1415417"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_5_2"/>
    <dbReference type="PhylomeDB" id="Q978P8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="8">
        <location>
            <begin position="15"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="7">
        <location>
            <begin position="128"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="6">
        <location>
            <begin position="242"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="376" mass="41212" checksum="FDBCD0783939D04E" modified="2001-10-01" version="1">MSQQKIEVIKMLCLSEEQSIIKGSIAEFTKRRIEPIEKRIEDDDYFPMDILREIGKNGFFSVMLPGIGVQDTYSFSLIAEEISKVSPEVAWLYVTHVAASYALDKLGTEEQKDSYLRKMADGEIIGTIAITESSSGATASAIASSARKYEENWILEGSKTFITMGGDADLYAVLAKTHEDPAAFTTFIVLKSDVGLELGTHLIGSAMKGIGWGEISFNNLILEKHRILGKENEGIKIIPTIARIFSIGAASISLGIMERALELVSSHVKERKIACKSLLSNQTVQDKLFHLYADTEAARSLVYRASINDDIRLAYSAKLFSTERSIAVTESAVSLFGATGYEESLAIPKLLNKAIAVPLHFINNAILTGLFITAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Q3</accession>
    <name>Q978Q3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="11">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">TVG1411343</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="11"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917596.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Q3"/>
    <dbReference type="SMR" id="Q978Q3"/>
    <dbReference type="STRING" id="273116.gene:9382170"/>
    <dbReference type="PaxDb" id="273116-14325601"/>
    <dbReference type="DNASU" id="1441478"/>
    <dbReference type="GeneID" id="1441478"/>
    <dbReference type="KEGG" id="tvo:TVG1411343"/>
    <dbReference type="eggNOG" id="arCOG01358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_4_2_2"/>
    <dbReference type="OrthoDB" id="372134at2157"/>
    <dbReference type="PhylomeDB" id="Q978Q3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="13">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="109"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="12">
        <location>
            <begin position="341"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAB60504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="401" mass="45520" checksum="1EE42C163087213B" modified="2001-10-01" version="1">MKIYFEAYGCTLNQGETALYVNKLLNEGNTLVSNPKEADLSIIGTCAVIKKTEDHMMARIQNLSRFGKVEVIGCLAPVKGKTMVEDNVSVIEKDRFRSFQEVIDEVSPVNAEIVSGIPINQGCTGKCNFCVSHIARGKLVSRKPEKIAGQIKILLQRGIKEIKITSLDTAAYGKDIGLRLPHLIRTITEIGDDFRLRVGMMEPKNTLEIVDDLISAYRSEKVFKFLHLPVQSGDDYVLEVMNREYTVSDFIRITGKFREAFPDSTLSTDLIIGYYAENDDSFEKTVQLIEKIRPEIINVTKFSPRELTADFEKRPKPTNILKEQDRMIADMHKEILQEKFSNLIGKTEFVLITENGKGRSMIGRDHAYRPIVIMDKTRKFEFHQVEITGFENTSLIGRIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Q6</accession>
    <name>Q978Q6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-oxoglutarate synthase subunit KorA</fullName>
            <ecNumber evidence="4">1.2.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-ketoglutarate oxidoreductase alpha chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">2-oxoglutarate-ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG1406112</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + 2-oxoglutarate + CoA = succinyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17297"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="1.2.7.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of the KorA, KorB, KorC and KorD subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.3" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917593.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Q6"/>
    <dbReference type="STRING" id="273116.gene:9382167"/>
    <dbReference type="PaxDb" id="273116-14325598"/>
    <dbReference type="GeneID" id="1441475"/>
    <dbReference type="KEGG" id="tvo:TVG1406112"/>
    <dbReference type="eggNOG" id="arCOG01606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_1_0_2"/>
    <dbReference type="OrthoDB" id="31112at2157"/>
    <dbReference type="PhylomeDB" id="Q978Q6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000022">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022367">
        <property type="entry name" value="2-oxoacid/accept_OxRdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03710">
        <property type="entry name" value="OAFO_sf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF20">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="9">
        <location>
            <begin position="14"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="10">
        <location>
            <begin position="214"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="11">
        <location>
            <begin position="476"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00052359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00064882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB60501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="585" mass="63979" checksum="DD061F862266DFCD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q978R5</accession>
    <name>Q978R5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1395957</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917584.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978R5"/>
    <dbReference type="STRING" id="273116.gene:9382158"/>
    <dbReference type="PaxDb" id="273116-14325589"/>
    <dbReference type="GeneID" id="1441466"/>
    <dbReference type="KEGG" id="tvo:TVG1395957"/>
    <dbReference type="eggNOG" id="arCOG00931">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_5_2"/>
    <dbReference type="PhylomeDB" id="Q978R5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF7">
        <property type="entry name" value="PQQA PEPTIDE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="313" mass="36665" checksum="36E53333AD6CB038" modified="2001-10-01" version="1">MKTMIWNIDYFCNLRCKSCNAWQGKIFFPEDLIDKHIEEMKKNGIKIVSVTGGEPTLNKSVVEIVKKLKEHHFFTHIATNGTNPYVMRKLYPYLDAVTISLDSDVPEEHNAYRGLKIFDTVVSTIKEAKNSVKIVTANMLVTNFNYFKVGRIADFANKELGVPLSMCFPEQDAYYFQDFHVTNDMIKQAFQYAYENYSRHVFGNMRSYYKDAVDYLEGRSSLQCKAGQEIFYTDYRGKVHPCFEHVEVVLNKKPEWDRYDNHCSQCFTECFLEPSIDRTFEAASLIGKIWWFNHVKKRDIRYVDTKRVKSIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q978T7</accession>
    <name>Q978T7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1371752</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917563.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978T7"/>
    <dbReference type="STRING" id="273116.gene:9382136"/>
    <dbReference type="PaxDb" id="273116-14325567"/>
    <dbReference type="GeneID" id="1441445"/>
    <dbReference type="KEGG" id="tvo:TVG1371752"/>
    <dbReference type="eggNOG" id="arCOG01708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_3_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="PhylomeDB" id="Q978T7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="113"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="220"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="369" mass="41322" checksum="17CDB5038311F397" modified="2001-10-01" version="1">MNFELDENLLNFRKKIEEFAKREFTEDLASKYDEQEHYPEDVFTKMMSEGIFDYTNPWNILMATEALCKYDAGMGISMLVPFFGNEVIALFGNDEQKKKYLSRVFAGKAKMGLAVTEPGGGSDVANIKTTAVKEGDHYVLNGSKMFITNGDIADFFVLLARTSQEKKKHHGMSVFIVERDFEGFRSNKIKGKLGVRATDTAELTLENVKVPEENLVGEPGRGFYYIMTFFDISRAYVAAQALGIAEGILERTLSILQKDGPLTSQPERVQFTIADMATRIEATKLLTYEAASYIFNFNPNPAVTSMAKAYAAETAVYVSRKAMEVTGIDGLTSSIERSFRDAKIMEIWEGTSEIEKLVIARMLKTGDVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q978W5</accession>
    <name>Q978W5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="10">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="10">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG1341378</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10 11">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="10"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917535.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978W5"/>
    <dbReference type="STRING" id="273116.gene:9382107"/>
    <dbReference type="PaxDb" id="273116-14325539"/>
    <dbReference type="GeneID" id="1441417"/>
    <dbReference type="KEGG" id="tvo:TVG1341378"/>
    <dbReference type="eggNOG" id="arCOG04184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082080_1_0_2"/>
    <dbReference type="OrthoDB" id="372108at2157"/>
    <dbReference type="PhylomeDB" id="Q978W5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000001">
        <property type="entry name" value="dITP/XTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011396">
        <property type="entry name" value="PRK14821.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00042">
        <property type="entry name" value="RdgB/HAM1 family non-canonical purine NTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="6"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="139"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="167"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB60442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="185" mass="21184" checksum="EDB6AF7AA2894413" modified="2001-10-01" version="1">MFKFVTSNKHKFEEIKELGTRYGIDIEWVNMKYEEIQEESTEKISYDSCKKLINLVDSPFFIDDTGLYIESLNGFPGPYSSYVSKTIGNYGILKLMNGIEKRSAYFVTVISLNEGGKITQFTGKVIGEISKEIRGKNGFGYDPIFIPNGSERTFAEMETSEKNMVSHRSMAFKGLFSYIKENYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q979B3</accession>
    <name>Q979B3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1288110</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979B3"/>
    <dbReference type="STRING" id="273116.gene:9382053"/>
    <dbReference type="PaxDb" id="273116-14325486"/>
    <dbReference type="KEGG" id="tvo:TVG1288110"/>
    <dbReference type="eggNOG" id="arCOG03651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_20_3_2"/>
    <dbReference type="PhylomeDB" id="Q979B3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="19"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="313" mass="34220" checksum="CC9D598787BCF5AA" modified="2001-10-01" version="1">MIGAVIIIIKVGNGNSLEPFSIPTNLGVGFSAIMFGSIFSILDFTGTGVVTTVSEEMREPKKNIGRSIVYAMILTAISIIPATYALTVGWGISNISSFASSPDAGIIVFEKYLGPIGAVLLIIFTINSYLTNGVSKATAIGRWWYSAASDGIVFPKRFSKVHSKYKSPYYAILTWSVSSFALDVIMGLYFGPKIAAFILEAGTGISIIIVHIMANTSLTLYTRRIDRFHFLKHALAPAFATVISLVVVYFTISDIWSKWVTHPNPVNDAYFASFIITLLWVIVGGLIVTLYYSRKKPNVLINAGEFDVENTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q979D1</accession>
    <name>Q979D1_THEVO</name>
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        <submittedName>
            <fullName evidence="1">TVG1267989 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1267989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979D1"/>
    <dbReference type="STRING" id="273116.gene:9382035"/>
    <dbReference type="PaxDb" id="273116-14325468"/>
    <dbReference type="KEGG" id="tvo:TVG1267989"/>
    <dbReference type="HOGENOM" id="CLU_2985892_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="57" mass="6830" checksum="80D56A3A71C52B57" modified="2001-10-01" version="1">MTYILYCAVMKQKFDKFYKLDIDFVKIRCIHGKNAYKKKRKIWFQKGLWGGAKLGPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q979F0</accession>
    <name>Q979F0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG1242712 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1242712</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760270.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979F0"/>
    <dbReference type="SMR" id="Q979F0"/>
    <dbReference type="STRING" id="273116.gene:9382014"/>
    <dbReference type="PaxDb" id="273116-14325449"/>
    <dbReference type="DNASU" id="1441325"/>
    <dbReference type="GeneID" id="1441325"/>
    <dbReference type="KEGG" id="tvo:TVG1242712"/>
    <dbReference type="eggNOG" id="arCOG01510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031172_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q979F0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004998">
        <property type="entry name" value="PRK06386.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF0">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="2">
        <location>
            <begin position="18"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="363" mass="40247" checksum="1DE4254AF3086FF6" modified="2001-10-01" version="1">MIGIVMLSKIANIDAARQGLDLKVKVLSLNKKTIKNDRGETTYFYGIIGDDTGTVSFTAWSFPAAVKSGDVVEIKNCYSSLYNGKIRIYVDSRSQVVLKPDEYLEVKRTVELVKLRDLSLTTPYVSVIGRISGIKEKNYDSDRGSKIVYQGFIEDETAKVRISSFGKPLKDGEIVRVENARVSQYNGYMGISIGENSSIDNVQAQIEVGSRPIFIAEIKSPIGGVTITGFAVSVGQGSRIFTKCSICKKILEDGKCKDHPRAPVYLDIFGYFLLSDGTGSLTCYLNKDSFLPYIKMDTEEFKSQAFSMNPNMLIKKNILGKCLSVTGDLRLRDDKITMNASSIKEADADSVKDIEKTIQEEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q979F1</accession>
    <name>Q979F1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1241900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917442.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979F1"/>
    <dbReference type="SMR" id="Q979F1"/>
    <dbReference type="STRING" id="273116.gene:9382013"/>
    <dbReference type="PaxDb" id="273116-14325448"/>
    <dbReference type="DNASU" id="1441324"/>
    <dbReference type="GeneID" id="1441324"/>
    <dbReference type="KEGG" id="tvo:TVG1241900"/>
    <dbReference type="eggNOG" id="arCOG02258">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1021648_0_0_2"/>
    <dbReference type="OrthoDB" id="56523at2157"/>
    <dbReference type="PhylomeDB" id="Q979F1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="271" mass="30436" checksum="8A22874030B4996D" modified="2001-10-01" version="1">MMNKREQDYWIFSVELRESKVSEENGKSIVITPLGLSAKRILIAGTVSSKQSDDRMARISLADPIGSFYVTAFSGGFNPEEKAMVDSLEVDDKAMVMGKINPYRTSEGVYLFSIRPELVSKTGEDALRLWTLKAYYFAKRRIYAIREAQKLQDPKADELISLGYSRVEAEAAISSIKNFPGYDYQRILEAIETAIYSVSTPTQLPEVRSKILNYIKENDTDGKGCKYEDIVIAAKNMGIDQSTTDEILNTLGSSGEIFEISLKRYKAVEPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-03" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q979F2</accession>
    <name>RPO5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo5</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo5</name>
        <name evidence="1" type="synonym">rpoH</name>
        <name type="ordered locus">TV1209</name>
        <name type="ORF">TVG1241478</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60351" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60351.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917441.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979F2"/>
    <dbReference type="SMR" id="Q979F2"/>
    <dbReference type="STRING" id="273116.gene:9382011"/>
    <dbReference type="PaxDb" id="273116-14325447"/>
    <dbReference type="GeneID" id="1441323"/>
    <dbReference type="KEGG" id="tvo:TVG1241478"/>
    <dbReference type="eggNOG" id="arCOG04258">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058320_4_0_2"/>
    <dbReference type="OrthoDB" id="30537at2157"/>
    <dbReference type="PhylomeDB" id="Q979F2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000146108" description="DNA-directed RNA polymerase subunit Rpo5">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="87" mass="9889" checksum="2A181CC3E758D886" modified="2002-04-03" version="2">MAKFNVLDHNLVPEHHIVSEEEEKTILKELGIEKEFLPRISPNDPVIKALEAIHGKIKDGTVIKIIRNSPTMGHSVYYRVVASEVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q979F4</accession>
    <name>Q979F4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit Rpo1N</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit A'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rpo1N</name>
        <name evidence="13" type="synonym">rpoA1</name>
        <name evidence="17" type="ORF">TVG1235237</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.</text>
    </comment>
    <comment type="function">
        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds at least 2 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13 14">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917439.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979F4"/>
    <dbReference type="STRING" id="273116.gene:9382009"/>
    <dbReference type="PaxDb" id="273116-14325445"/>
    <dbReference type="GeneID" id="1441321"/>
    <dbReference type="KEGG" id="tvo:TVG1235237"/>
    <dbReference type="eggNOG" id="arCOG04257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000487_3_1_2"/>
    <dbReference type="OrthoDB" id="371812at2157"/>
    <dbReference type="PhylomeDB" id="Q979F4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02582">
        <property type="entry name" value="RNAP_archeal_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00863">
        <property type="entry name" value="RNApol_arch_Rpo1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012758">
        <property type="entry name" value="RPO1N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02390">
        <property type="entry name" value="RNA_pol_rpoA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF32">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 13 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="16">
        <location>
            <begin position="207"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="665"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAB60349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="886" mass="100023" checksum="17AF947159999471" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q979F9</accession>
    <name>Q979F9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1231911</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979F9"/>
    <dbReference type="STRING" id="273116.gene:9382003"/>
    <dbReference type="PaxDb" id="273116-14325440"/>
    <dbReference type="KEGG" id="tvo:TVG1231911"/>
    <dbReference type="eggNOG" id="arCOG04150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064992_3_0_2"/>
    <dbReference type="OrthoDB" id="7870at2157"/>
    <dbReference type="PhylomeDB" id="Q979F9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22390">
        <property type="entry name" value="KH-I_Dim2p_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019964">
        <property type="entry name" value="KH_domain_protein_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03665">
        <property type="entry name" value="arCOG04150"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK13763.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
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    </dbReference>
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        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="81"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="182" mass="20170" checksum="772AB2BB11631C68" modified="2001-10-01" version="1">MFLEAIRVPKDRIKVVIGKDGEVKKKIEEMGEVKLTIDTAEAEVSIDQLGDAVKSSIAKNVVQAIGRGFNPGKAMLLFEENMQLVIISLREFAKPGSSKITQIKARVIGTGGKTRAIIEELTGSYLSVYGDTISIIGDYLAVTYAEEAINMIINGKKHRTVYAFLEKRARELKYKRIEESFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q979J2</accession>
    <name>Q979J2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transport system permease protein SP1P2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1197131</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917402.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979J2"/>
    <dbReference type="STRING" id="273116.gene:9381970"/>
    <dbReference type="PaxDb" id="273116-14325407"/>
    <dbReference type="GeneID" id="1441284"/>
    <dbReference type="KEGG" id="tvo:TVG1197131"/>
    <dbReference type="eggNOG" id="arCOG00157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_2"/>
    <dbReference type="PhylomeDB" id="Q979J2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051393">
        <property type="entry name" value="ABC_transporter_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30193">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30193:SF37">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER PERMEASE PROTEIN YCJO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="87"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="309" mass="34930" checksum="E2A06902A4FDC1BC" modified="2001-10-01" version="1">MVCLNTEVYQVNKIREGKKKNKSPEKMTGILFIIPLMIFISVFTFFPAIYSFVISLFHYNPFFHSIYFTGVSNYIHVVESPAFLKSLLNVLLYTAVVVTVQTFLAFVYALLFNTASKISRIARAIVFIPAVVSPVSMSIIFIWVFSDSGLVNFFLSFFGIAPHNWLFSTEFAFPAIMAMNIFTTAPYFMIIYSAGLQAIPQDVLDAAKIDGITSAFARFRYIYFPMMRFSTVLVVILGLTGAMQLFDQIYVITDGGPARSTYVPLMYIYNRTFVYIGDIGLAAAASFVLFVIIMALTITQRKVITERTW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q979L4</accession>
    <name>METE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine synthase</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Homocysteine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metE</name>
        <name type="ordered locus">TV1147</name>
        <name type="ORF">TVG1176554</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a methyl group to L-homocysteine resulting in methionine formation. The physiological methyl donor is unknown.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the archaeal MetE family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60289" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60289.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917381.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979L4"/>
    <dbReference type="SMR" id="Q979L4"/>
    <dbReference type="STRING" id="273116.gene:9381946"/>
    <dbReference type="PaxDb" id="273116-14325385"/>
    <dbReference type="DNASU" id="1441263"/>
    <dbReference type="GeneID" id="1441263"/>
    <dbReference type="KEGG" id="tvo:TVG1176554"/>
    <dbReference type="eggNOG" id="arCOG01876">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040013_3_2_2"/>
    <dbReference type="OrthoDB" id="17656at2157"/>
    <dbReference type="PhylomeDB" id="Q979L4"/>
    <dbReference type="UniPathway" id="UPA00051"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00288">
        <property type="entry name" value="MetE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022921">
        <property type="entry name" value="MetE_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002272">
        <property type="entry name" value="PRK01207.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30519">
        <property type="entry name" value="5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000098695" description="Methionine synthase">
        <location>
            <begin position="1"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="344" mass="40153" checksum="7EF4BEE284F93902" modified="2002-10-10" version="2">MAALITQEIGSFRKPEYLSMEFHKIEGTEKFKELAERATIDTLKIFENTGLDNVGIGGEMYRWEMYEHPAERIKGLIFYGMVRSFDNRYYRKGSVIDKIERRGSFHMDEVEFVAKSTKKPIKIPITGPYTMMDWSFNDHYNDRHELAMEFARIINEELKEIQSKWPYISNGRKLEIQIDEPATTTHPDEMDIVVDSVNKSIEGIDAELSLHVCYSRDYRLLYDRIPELNIDGYNLEYSNRDTTDLGVEDAKRPGFQDIKYFNEVNESLQRKKFIGVGVTDVHIDFIEPVKLIEDRIKYVLNIIKDPELVKLNPDCGLRTRSRSIGEQKLRNMVIAKNNVLKDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q979M8</accession>
    <name>Q979M8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase I chain H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1162018</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 4L family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917366.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979M8"/>
    <dbReference type="STRING" id="273116.gene:9381931"/>
    <dbReference type="PaxDb" id="273116-14325370"/>
    <dbReference type="GeneID" id="1441248"/>
    <dbReference type="KEGG" id="tvo:TVG1162018"/>
    <dbReference type="eggNOG" id="arCOG03073">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144724_1_1_2"/>
    <dbReference type="OrthoDB" id="56168at2157"/>
    <dbReference type="PhylomeDB" id="Q979M8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030964">
        <property type="term" value="C:NADH dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01456">
        <property type="entry name" value="NDH1_NuoK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001133">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chain4L/K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004320">
        <property type="entry name" value="PRK05715.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434:SF16">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT ND4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="103" mass="11199" checksum="7483994D43FC0478" modified="2001-10-01" version="1">MFAAAVSFVALLLFTIGLYGVMTSRVGIKMLISIEIMINSAILSIIGIGSYFYSSSVQIDPYVFALFAIAIGVVESAVGLGLLVIVYRKFGKIDISLLKEIRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q979M9</accession>
    <name>Q979M9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase I chain I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1161017</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917365.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979M9"/>
    <dbReference type="STRING" id="273116.gene:9381930"/>
    <dbReference type="PaxDb" id="273116-14325369"/>
    <dbReference type="GeneID" id="71201328"/>
    <dbReference type="KEGG" id="tvo:TVG1161017"/>
    <dbReference type="eggNOG" id="arCOG01539">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q979M9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829:SF2">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal" evidence="7">
        <location>
            <begin position="65"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="6">
        <location>
            <begin position="125"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="332" mass="36912" checksum="E25BFB4045728413" modified="2001-10-01" version="1">MFVYGWMIFIAPLLGFVFSLVIGKFYRKWSGAVASFFIFLALVFAILSYFQVLKGPIYNSYHWFYNIDYGIYIDNLAITMAIMVSFVSLMIHLFAIYYMKDDPNKHVYFAETSLFTAGMLGLVISSNLVLLFLFWELVGLCSYLLIGFWFFKPNATAAAKKAFIVTRVGDLSFIIGMSILYYSLVNVTGDPLSIPYLISHASLIASQIGKVRLGIIAIFILGAAIGKSAQFPLHVWIPDAMEGPTTVSALIHAATMVTAGVYLVARLYQVFLYAAPFAMYAVAIIGSFTALYAGILGLVVNDVKRILAYSTISQLGYRLIYCTLCRHPWPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q979N6</accession>
    <name>Q979N6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="3 13">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">TVG1155064</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="3 13"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917358.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979N6"/>
    <dbReference type="STRING" id="273116.gene:9381923"/>
    <dbReference type="PaxDb" id="273116-14325362"/>
    <dbReference type="GeneID" id="1441240"/>
    <dbReference type="KEGG" id="tvo:TVG1155064"/>
    <dbReference type="eggNOG" id="arCOG04300">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_0_2"/>
    <dbReference type="OrthoDB" id="8493at2157"/>
    <dbReference type="PhylomeDB" id="Q979N6"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00384">
        <property type="entry name" value="ALAD_PBGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000019">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAB60266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="321" mass="36067" checksum="FF5B3FB14A3F38DC" modified="2001-10-01" version="1">MYPIVRMRRYRKDQNLRDFISEIRVSIDKLIMPIFIDENIKEKTQISSMPGIFRYPISQLEDYVKHLEDIGIKSVLLFGIPKYKDSFGSSAYDKNSIIQRSISLIKETTDIVTIADLCLCEYTDTGQCGLISNDYVDNDKTLEVYRKIAVSYAEAGVDIVAPSGMMDGQVKAIREALDSSGYVNTMIMAYSSKFSSNLYGPFREAAESAPKVGDRKSYQLDYRNGKEAIREIELDIDEGADIVMVKPAIFYLDIIRSARDRFNLPLAAYSVSGEYSMIVNAVNQGLIGEDSVNEALTAVFRAGADIVITYFAERLAESRKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Q3</accession>
    <name>TF2B1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription initiation factor IIB 1</fullName>
            <shortName evidence="1">TFIIB 1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tfbA</name>
        <name type="ordered locus">TV1107</name>
        <name type="ORF">TVG1139885</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917341.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Q3"/>
    <dbReference type="SMR" id="Q979Q3"/>
    <dbReference type="STRING" id="273116.gene:9381906"/>
    <dbReference type="PaxDb" id="273116-14325345"/>
    <dbReference type="GeneID" id="1441223"/>
    <dbReference type="KEGG" id="tvo:TVG1139885"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="PhylomeDB" id="Q979Q3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000119340" description="Transcription initiation factor IIB 1">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="129"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="223"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TFIIB-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <sequence length="312" mass="35266" checksum="FBFA56FB1CA9E11A" modified="2001-10-01" version="1">MVENQKKKKIEEIERCPECGSTNLIRDYEHGELVCGECGAVIEDSYIDQGPEWRAFDSEQNESRARAGSPMTFTIHDKGLSTDISWKNKDSYGRSIPTRNRAQLYRLRKWQKRIKVSNAAERNLSQALQELERMAFNLSIPNDVRETAAVIYRKAVKQNMIRGRSIEGVVAGALYAACRITNVPRTLGEIASVTRVKKKEIGRTYRIMSRYLKLNIMPSKAEDYISRFCSKLKLSMDTRNKALEILRDAENVGLTSGKGPTGVAAAAIYIASLITGERRTQRAVAEVAGVTEVTIRNRYKELTEKLKLNVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q979S1</accession>
    <name>Q979S1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1119628 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1119628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917321.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979S1"/>
    <dbReference type="STRING" id="273116.gene:9381886"/>
    <dbReference type="PaxDb" id="273116-14325327"/>
    <dbReference type="GeneID" id="1441203"/>
    <dbReference type="KEGG" id="tvo:TVG1119628"/>
    <dbReference type="eggNOG" id="arCOG07396">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_996134_0_0_2"/>
    <dbReference type="OrthoDB" id="57110at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="279" mass="31158" checksum="3100BCF57235D3E6" modified="2001-10-01" version="1">MAYADYGKWAIVVNIGVMAPPLAILLYVMPRTYIYANIAVFLIFSAAPFIRRILRKPFMDLVYPIAVLVVSASIIIGLLLREGLVKNKYYVLATQLNEPFMVSISLAVAVITLVEGIFATKLYSVITLMAFSTLSTLDLLAALTVQSITGYPFFTSFFYVELMEYEALFTLVFYGYEYSLPLATFAVPINSELIAAFIISLASIIVRSYMNGNRSIDRIRSFAFSIFYGSIIGVAAVYVVNVSESFNLQFLLISVIILLMVYFVIKTSKPIKKKDRNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q979S7</accession>
    <name>Q979S7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative arsenical pump-driving ATPase</fullName>
            <ecNumber evidence="8">7.3.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Arsenical resistance ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Arsenite-translocating ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Arsenite-transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">TVG1112685</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Anion-transporting ATPase. Catalyzes the extrusion of arsenite.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>arsenite(in) + ATP + H2O = arsenite(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11348"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29242"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.7" evidence="8"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917314.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979S7"/>
    <dbReference type="STRING" id="273116.gene:9381879"/>
    <dbReference type="PaxDb" id="273116-14325321"/>
    <dbReference type="GeneID" id="1441196"/>
    <dbReference type="KEGG" id="tvo:TVG1112685"/>
    <dbReference type="eggNOG" id="arCOG02849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_1_0_2"/>
    <dbReference type="OrthoDB" id="46198at2157"/>
    <dbReference type="PhylomeDB" id="Q979S7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015446">
        <property type="term" value="F:ATPase-coupled arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00298">
        <property type="entry name" value="ACD_sHsps_p23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001801">
        <property type="entry name" value="Putative arsenical pump-driving ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040612">
        <property type="entry name" value="ArsA_HSP20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17886">
        <property type="entry name" value="ArsA_HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0059">Arsenical resistance</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="13">
        <location>
            <begin position="4"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="ArsA HSP20-like" evidence="14">
        <location>
            <begin position="325"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAB60225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="387" mass="43872" checksum="C5F66CAD384B4D78" modified="2001-10-01" version="1">MKPRILLYTGKGGVGKTSIAAATGSLLAAEGKKTLIISTDPAHSLGDAFGMEIGHNIKKLGENLYGQEVSVVQSINEHWGELKDYLRSLFLSQGLDPVSADEIATLPGFEEASELLYLRNYYYDEEYDTIVMDSAPTGAALQLLSFPEVMTWYMDKLFPLGRKTARVARPILKPFVDIPLPDDEVFENIDLLYKQLTDIRKILTNNEVTSIRLVTNPDNMSFSETKRAYTYLLLYGYPVDAVVINKILSDETGGFFEKMRVNQQDIIDEMEHSFVDVKVFKVKLLQEEPIGHEKLVELGKLIYGDEDPYKIFYKGTPIKYSKSGEKYILKIKMPFAAKENINLFNHGGELTIEIENWKRVFYLPESISDRRPVSAEYSNGYLNVVLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-13" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q979T2</accession>
    <name>RS10_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps10</name>
        <name type="ordered locus">TV1078</name>
        <name type="ORF">TVG1106762</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917308.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979T2"/>
    <dbReference type="SMR" id="Q979T2"/>
    <dbReference type="STRING" id="273116.gene:9381874"/>
    <dbReference type="PaxDb" id="273116-14325316"/>
    <dbReference type="GeneID" id="1441190"/>
    <dbReference type="KEGG" id="tvo:TVG1106762"/>
    <dbReference type="eggNOG" id="arCOG01758">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_0_1_2"/>
    <dbReference type="OrthoDB" id="371736at2157"/>
    <dbReference type="PhylomeDB" id="Q979T2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000004">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_uS10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000146663" description="Small ribosomal subunit protein uS10">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="104" mass="11826" checksum="C33458604D50F1BD" modified="2001-10-01" version="1">MASYKARISLSGTEHKIVDMVCSEIKEIASRTGVEIHGPMPLPTKKLVVPVRKSPDGEGTNTWDHWEMRIHKRLIDVDADERTLRQLMRIPIPDGVQIEIQIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q979U9</accession>
    <name>Q979U9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG1084690</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917290.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979U9"/>
    <dbReference type="STRING" id="273116.gene:9381855"/>
    <dbReference type="PaxDb" id="273116-14325299"/>
    <dbReference type="GeneID" id="1441172"/>
    <dbReference type="KEGG" id="tvo:TVG1084690"/>
    <dbReference type="eggNOG" id="arCOG00439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_6_0_2"/>
    <dbReference type="OrthoDB" id="6747at2157"/>
    <dbReference type="PhylomeDB" id="Q979U9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000826">
        <property type="entry name" value="Replicative DNA helicase Mcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="10">
        <location>
            <begin position="287"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB60203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="699" mass="78908" checksum="86F55084EE6C92FD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q979X2</accession>
    <name>Q979X2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1062110</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelA/SpoT family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917267.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979X2"/>
    <dbReference type="STRING" id="273116.gene:9381832"/>
    <dbReference type="PaxDb" id="273116-14325276"/>
    <dbReference type="GeneID" id="1441149"/>
    <dbReference type="KEGG" id="tvo:TVG1062110"/>
    <dbReference type="eggNOG" id="arCOG00357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037276_1_0_2"/>
    <dbReference type="OrthoDB" id="5875at2157"/>
    <dbReference type="PhylomeDB" id="Q979X2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01669">
        <property type="entry name" value="TGS_MJ1332_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000002">
        <property type="entry name" value="GTP pyrophosphokinase (RelA/SpoT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007171">
        <property type="entry name" value="PRK09602.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="3"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="382" mass="42916" checksum="DE8D1F1342211F53" modified="2001-10-01" version="1">MSIRIGLIGEPNVGKSTFFSAATENEAEINNFPFTTIKPNLGMTYFVVKCPEVEISGKCNPREGYCENGNRHIPVQIIDVPGLIEGASEGKGMGNEFLDNIRDVDSILLLFDASAGDIATVRKSIEMVKSELLKWISQRIYSDWDHFSRKSEASGERRDIALKRKLSLFGISEHQISEMMEKDFFPAKLSIWTREDADKLALSIIKYAKPLLLVGNKSDLVSPNTLSEMKNALGDSYFVSAEYELALKRARKSGLVSTTLNDFAILGGNEKQKEVLAKIRDWFNTPGIIRIHDLLQKVVRDYLHYIIVYPVYDESKWTDKSGNVLPDAFVMPYGSTSLDLAYKIHTDIGEGFIRAVNARTRMVLGKDYILKDGDVIKIISKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q979X3</accession>
    <name>Q979X3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1061681 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1061681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979X3"/>
    <dbReference type="STRING" id="273116.gene:9381831"/>
    <dbReference type="PaxDb" id="273116-14325275"/>
    <dbReference type="KEGG" id="tvo:TVG1061681"/>
    <dbReference type="eggNOG" id="arCOG02761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126998_1_1_2"/>
    <dbReference type="PhylomeDB" id="Q979X3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14797">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.70">
        <property type="entry name" value="TT1751-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005180">
        <property type="entry name" value="DUF302"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035923">
        <property type="entry name" value="TT1751-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016796">
        <property type="entry name" value="UCP021774"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342">
        <property type="entry name" value="SLR5037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342:SF1">
        <property type="entry name" value="SLR5037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03625">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021774">
        <property type="entry name" value="UCP021774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103247">
        <property type="entry name" value="TT1751-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF302" evidence="1">
        <location>
            <begin position="51"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="142" mass="16224" checksum="DE892554C8F5DF56" modified="2001-10-01" version="1">MALHIPPPLRKMQKSSFMYKYERKVNGTVEEVYGKLFTELKKRGYVVLSYIDVAQILKNALKKDVDPYYILNVCKPSAADEILSVSRDYGLFLPCKIVLKGEGDKVTISMQYVSTQINDYMGQDGNVAVKYEKEIEEAIKDI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2017-03-15" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Z2</accession>
    <name>TVOK_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcium-gated potassium channel TvoK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tvoK</name>
        <name evidence="6" type="ordered locus">TV1018</name>
        <name evidence="7" type="ORF">TVG1041141</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="J. Biol. Chem." volume="282" first="24302" last="24309">
            <title>Molecular architecture and divalent cation activation of TvoK, a prokaryotic potassium channel.</title>
            <authorList>
                <person name="Parfenova L.V."/>
                <person name="Abarca-Heidemann K."/>
                <person name="Crane B.M."/>
                <person name="Rothberg B.S."/>
            </authorList>
            <dbReference type="PubMed" id="17588939"/>
            <dbReference type="DOI" id="10.1074/jbc.M703650200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>ALTERNATIVE INITIATION</scope>
        <scope>MUTAGENESIS OF ARG-111; LYS-114; LEU-115 AND LYS-117</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Calcium-gated potassium channel. Can also be activated by Mg(2+), Mn(2+) and Ni(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterooctamer composed of four full-length subunits and four soluble RCK domains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="alternative initiation"/>
        <isoform>
            <id>Q979Z2-1</id>
            <name evidence="6">1</name>
            <name evidence="6">Calcium-gated potassium channel</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q979Z2-2</id>
            <name evidence="6">2</name>
            <name evidence="6">Soluble RCK domain</name>
            <sequence type="described" ref="VSP_059160 VSP_059161"/>
        </isoform>
    </comment>
    <comment type="miscellaneous">
        <molecule>Isoform 2</molecule>
        <text evidence="4">Starts at UUG codon.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917246.1">
        <molecule id="Q979Z2-1"/>
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Z2"/>
    <dbReference type="SMR" id="Q979Z2"/>
    <dbReference type="STRING" id="273116.gene:9381811"/>
    <dbReference type="TCDB" id="1.A.1.13.3">
        <property type="family name" value="the voltage-gated ion channel (vic) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325256"/>
    <dbReference type="GeneID" id="1441128"/>
    <dbReference type="KEGG" id="tvo:TVG1041141"/>
    <dbReference type="eggNOG" id="arCOG01958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050982_1_1_2"/>
    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="PhylomeDB" id="Q979Z2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF9">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN YUGO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0024">Alternative initiation</keyword>
    <keyword id="KW-0106">Calcium</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-0631">Potassium channel</keyword>
    <keyword id="KW-0633">Potassium transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000439237" description="Calcium-gated potassium channel TvoK">
        <location>
            <begin position="1"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="2">
        <location>
            <begin position="120"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="3">
        <location>
            <begin position="266"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_059160" description="In isoform 2." evidence="4">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_059161" description="In isoform 2.">
        <original>L</original>
        <variation>M</variation>
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces levels of the soluble RCK domain." evidence="4">
        <original>R</original>
        <variation>N</variation>
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Does not affect levels of the soluble RCK domain." evidence="4">
        <original>K</original>
        <variation>N</variation>
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes expression of the soluble RCK domain." evidence="4">
        <original>L</original>
        <variation>N</variation>
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Does not affect levels of the soluble RCK domain." evidence="4">
        <original>K</original>
        <variation>N</variation>
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="17588939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="17588939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="348" mass="38605" checksum="706E08967EABA376" modified="2001-10-01" version="1">MRPFRTLVKVLQKIESSPLTKVFMAFIIVVLIGSYLEFLTQRNVKYSEIKNYFTAIWFTMETVTTVGYGDVVPVSNLGRVVAMLIMVSGIGLLGTLTATISAYLFQIRIERRGKLEKRLKNHTIICNWNAYTRNIIEGNRDEEAAPIVILSENTDNSEKYQNVFFVKGDCTSEDDLKNAAIEDAARVVIMSDIENNAIPEDLLDAKTLLSIFTVRKLNNAVEIIAEVRDEKNKKHAVSAGATEVLSVGELSSRLISRSISNPGLTDFILKSLSETEDFKIFSIEACGILPGMTVKDAASNLAEVCVIISVISKGKIEYPPDRSYKIGAGDYVVFLAKNKKEVEEAIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A00</accession>
    <name>Q97A00_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ferripyochelin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1032219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917238.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A00"/>
    <dbReference type="STRING" id="273116.gene:9381803"/>
    <dbReference type="PaxDb" id="273116-14325248"/>
    <dbReference type="GeneID" id="1441120"/>
    <dbReference type="KEGG" id="tvo:TVG1032219"/>
    <dbReference type="eggNOG" id="arCOG01849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064827_4_1_2"/>
    <dbReference type="OrthoDB" id="10940at2157"/>
    <dbReference type="PhylomeDB" id="Q97A00"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04645">
        <property type="entry name" value="LbH_gamma_CA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047324">
        <property type="entry name" value="LbH_gamma_CA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050484">
        <property type="entry name" value="Transf_Hexapept/Carb_Anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061">
        <property type="entry name" value="DYNACTIN SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061:SF29">
        <property type="entry name" value="GAMMA CARBONIC ANHYDRASE-LIKE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="173" mass="19041" checksum="1A750DDB821C7524" modified="2001-10-01" version="1">MIYEFEGRVPDIDPSAYVSESATVIGKVKIGKEVWIGPGAVLRGDYGEIEVGDYSAIEDNCVIHARPGEKTFIGQHVTIGHLSVIHTGRIRDWAVIGMGSTVSDFAVVGVWAAIGEGAVVKNRQEIPDESIAVGVPAKVIGKIDEDYKKLWTDYKHNYNTFSSRYKTNLKKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A29</accession>
    <name>Q97A29_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cas12f1-like TNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1001758</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760259.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A29"/>
    <dbReference type="SMR" id="Q97A29"/>
    <dbReference type="STRING" id="273116.gene:9381773"/>
    <dbReference type="PaxDb" id="273116-14325198"/>
    <dbReference type="GeneID" id="1442058"/>
    <dbReference type="KEGG" id="tvo:TVG1001758"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_791354_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97A29"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="3">
        <location>
            <begin position="281"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="386" mass="44528" checksum="BB8A83B406CE022A" modified="2001-10-01" version="1">MYAFKAVKQYFYPSDNLKVLMHKFTDMVNFTINIMIEKNLTSRNSVSNEVYHKLKEYEIPSYYYIEAINKAVALVKTYRKRLKKKQKATVPHVEKPMLSTYYGFKIVGGNLMIPIANREYESIPLNAYVRDATSMVKVHSFALSAYTLSLTIGRDVDAIECTTTVGVDRNLRNATVGNEFHHEIYDLSNVVEIKQRYRKKESHFHRNDRRIREKILSKYGKRERNRVKQIMHETSKAIVSNASTRKEMIVLENIRGINKITKKGDGKGKDYRFLLKNAFPYGMLASQVMYKASWEGLPVIELTRKETRNTSRMCSVCGSLTRIEHGRILKCDSCGLEIDRDVNACINIAFRYRTCLKRSHKGLPGEAVKQSKDVEQMAGSQISTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
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            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A36"/>
    <dbReference type="STRING" id="273116.gene:9381766"/>
    <dbReference type="PaxDb" id="273116-14325191"/>
    <dbReference type="GeneID" id="55661515"/>
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    <dbReference type="eggNOG" id="arCOG05368">
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    <dbReference type="OrthoDB" id="52587at2157"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="BAB60116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="53" mass="6236" checksum="D23A94D5EB0348CF" modified="2001-10-01" version="1">MEELICNIEFSKESDTEYVAKLRTEMGGLREYTGRTFEDVLNQVIIELEEEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A59</accession>
    <name>Q97A59_THEVO</name>
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            <taxon>Thermoplasma</taxon>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
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            <dbReference type="PubMed" id="11121031"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1286412_0_0_2"/>
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    <dbReference type="UniPathway" id="UPA00262">
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Met8-like"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="214" mass="24272" checksum="D8EE26E13C5DC4FD" modified="2001-10-01" version="1">MIVDLRLSKSTSLFIGHGKEFRDKFELYHSEFGTVYVISDEVLLEDANVIRVSSSFEDSYDAIESVKPFLAFISTEDRDLDTKLAYFARRHSKLVYVPDRNDLNDINLCAILQYGPIKIAVSTSGKSPAMTVMVKKRIAASIKKYALIDNMDQSVVEAIARNREKIISSVKEPKKRRIMMYRIAVDRRIREYSVLNPVMADEAIAKILNGAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A81</accession>
    <name>Q97A81_THEVO</name>
    <protein>
        <submittedName>
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    </protein>
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            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A81"/>
    <dbReference type="PaxDb" id="273116-14325146"/>
    <dbReference type="KEGG" id="tvo:TVG0957188"/>
    <dbReference type="HOGENOM" id="CLU_1324082_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="207" mass="23560" checksum="B2D4B17BB87FE5A8" modified="2001-10-01" version="1">MSIFLKERWYQKIKTGCYVICSTLSINQRLLCFCLRNSLILQIIIQPLLFTLSNFSYNRCQFTATRLFSCFIQWINNLSSCSIISDLRVSYEQIKLEDTNIYSPFFLRCYSLLIVFSIRIIRNIFLEKINYWAIASTLNLEVLGPNSARSKTTIAMNTHISPKDASSPCGRKKAVINGTSIDVDILLTLYTVPAAVGLTLVGKISAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A82</accession>
    <name>Q97A82_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0956419 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0956419</name>
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    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054007.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A82"/>
    <dbReference type="PaxDb" id="273116-14325145"/>
    <dbReference type="DNASU" id="1442012"/>
    <dbReference type="GeneID" id="1442012"/>
    <dbReference type="KEGG" id="tvo:TVG0956419"/>
    <dbReference type="eggNOG" id="arCOG02283">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1248231_0_0_2"/>
    <dbReference type="OrthoDB" id="168808at2157"/>
    <dbReference type="PhylomeDB" id="Q97A82"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="160"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="217" mass="24722" checksum="6F1E58BC621459BF" modified="2001-10-01" version="1">MMYYLSFSISSNCGLSAAAEHLQNKIVTLNVINSTDFNTVIAFTLARDIDAMRNDFAKDFKTVYIARGKKAAFINGTKISHGIVPAMISEQIIPLYPIFAENGSEWFNAIVLEKEHIDNFVALIEKKNNIETLDYERIRNGDSMFSIIKNANRGIFTLELTETERRIVNSSLRQGFFSWPRSYDLTSISREFGLTKPTILYHIRNAERKVMEALFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AA5</accession>
    <name>Q97AA5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NDP-sugar epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0926940</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917145.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AA5"/>
    <dbReference type="STRING" id="273116.gene:9381697"/>
    <dbReference type="PaxDb" id="273116-14325122"/>
    <dbReference type="GeneID" id="1441993"/>
    <dbReference type="KEGG" id="tvo:TVG0926940"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="PhylomeDB" id="Q97AA5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078:SF6">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01713">
        <property type="entry name" value="NUCEPIMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="5">
        <location>
            <begin position="8"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="307" mass="34819" checksum="A7126DB60D67C9EF" modified="2001-10-01" version="1">MAIENKEILVTGGAGFIGSNLVARLSEKNHVYVLDSLQTGSLHNIDGLSNVEFIKDYAKNVSNYCINPDYIFHIGVYSSSPMYRSNPFLVSEAIHDMIAILELAKKSKTPVVFASTSSIYNGVKPPHRENVTPLVSDYYTEARISMERISELYSKLYDINISAMRFFSIYGYREMKKRMYANLVSQFLWDMHSKKSPIIYGDGEQRRDFVFVDDVVDALILAAEKSDGFQVYNVGTGVNYSLNELVQKLNKYLGSNIEPKYVENPMAKTYVHETLADTNKAEKMIGFRAKISLDEGIKRLISYYGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AC3</accession>
    <name>Q97AC3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0904250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AC3"/>
    <dbReference type="STRING" id="273116.gene:9381679"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325104"/>
    <dbReference type="KEGG" id="tvo:TVG0904250"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891557_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AC3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="7"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="134" mass="15282" checksum="0AB16D119BD8B677" modified="2001-10-01" version="1">MDEIPADIRNEIIYYYRLSRKVLLGLYNKSIIFVLPSIVEGMPSPPLEAMACGCAVVVTDNGGVNEYIKDGLNGIVCPVRDSYCLYQKVILLINNKALREQMIQDGLETAKEFSYDNMNKNFIRLIEEVQRRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AE9</accession>
    <name>Q97AE9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">2-ketovalerate ferredoxin oxidoreductase [VOR] beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG0882664</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AE9"/>
    <dbReference type="STRING" id="273116.gene:9381653"/>
    <dbReference type="PaxDb" id="273116-14325078"/>
    <dbReference type="KEGG" id="tvo:TVG0882664"/>
    <dbReference type="eggNOG" id="arCOG01599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AE9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03375">
        <property type="entry name" value="TPP_OGFOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053399">
        <property type="entry name" value="2-oxoacid:Fd_oxidored_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051457">
        <property type="entry name" value="2-oxoacid:Fd_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011896">
        <property type="entry name" value="OFOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032686">
        <property type="entry name" value="PFO_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041171">
        <property type="entry name" value="Oxoac_fdxbeta_Archa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02177">
        <property type="entry name" value="PorB_KorB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084:SF2">
        <property type="entry name" value="PYRUVATE FERREDOXIN_FLAVODOXIN OXIDOREDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12367">
        <property type="entry name" value="PFO_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="10">
        <location>
            <begin position="48"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate ferredoxin oxidoreductase beta subunit C-terminal" evidence="11">
        <location>
            <begin position="199"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB60003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="309" mass="33960" checksum="9BA331FF9334B345" modified="2001-10-01" version="1">MKMVHNFRNDITVDWCPGCGDFGILTALTSALSELNLGSHDVAIISGIGCSGKTPHYVNAAGAHTLHGRAIPVAVGVKLTNPHLKVIVTGGDGDLMSIGAGHLVAEGRRNSGITVLMYDNAVYGLTKGQAAPTLKLGVQTKSLARPNIYDAINPIMLAISSGYSFVARGFSFEIAHLKNIIKQAIMFPGSSFIDILQPCPTYNNINTMDWYKKRVYKLDDDKSWDPVITENDPKAEEKYNKAIEKSFEWGDKIPIGVFYVNKKVPPFTERLKQYVDNYEQIPPAVQDVSTEDGKPILDPFETFKDKIIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AF1</accession>
    <name>NADM_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV0859</name>
        <name type="ORF">TVG0879099</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60001" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60001.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917103.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AF1"/>
    <dbReference type="SMR" id="Q97AF1"/>
    <dbReference type="STRING" id="273116.gene:9381651"/>
    <dbReference type="PaxDb" id="273116-14325076"/>
    <dbReference type="GeneID" id="1441951"/>
    <dbReference type="KEGG" id="tvo:TVG0879099"/>
    <dbReference type="eggNOG" id="arCOG00972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108783_0_0_2"/>
    <dbReference type="OrthoDB" id="264480at2157"/>
    <dbReference type="PhylomeDB" id="Q97AF1"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02166">
        <property type="entry name" value="NMNAT_Archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01527">
        <property type="entry name" value="arch_NMN_Atrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002243">
        <property type="entry name" value="PRK01153.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF0">
        <property type="entry name" value="BIFUNCTIONAL NMN ADENYLYLTRANSFERASE_NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342">
        <property type="entry name" value="PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000135008" description="Nicotinamide-nucleotide adenylyltransferase">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="178" mass="20778" checksum="714929EBCD94AB3E" modified="2002-10-25" version="2">MQSTKEHRAFLIGRFQPFHLGHLEIVKRILRENDSIIIGIGSAQYSHTTVNPFTAGERHLMISRTLEREHVYNYYLVPIEDVNANSLWVSHVEALAPKFDVVYTNNPLVRRLFTEKHYEVRSLPMVNRSEWTGTKIREKMIKGENWEQNVPEPVVEVIREIDGISRIRQLSTTDEDVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AH2</accession>
    <name>Q97AH2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ornithine cyclodeaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0859714</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917082.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AH2"/>
    <dbReference type="STRING" id="273116.gene:9381630"/>
    <dbReference type="PaxDb" id="273116-14325055"/>
    <dbReference type="DNASU" id="1441930"/>
    <dbReference type="GeneID" id="1441930"/>
    <dbReference type="KEGG" id="tvo:TVG0859714"/>
    <dbReference type="eggNOG" id="arCOG01035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_2_1_2"/>
    <dbReference type="OrthoDB" id="21421at2157"/>
    <dbReference type="PhylomeDB" id="Q97AH2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005011">
        <property type="entry name" value="PRK06407.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="301" mass="33365" checksum="92B18AAB73198FFE" modified="2001-10-01" version="1">MYYVTEDDVDKNFDMKAAINAVREAFEEYGKGRAYSSTRVRTFSQDGVLNTMPAAMEKYHISGLKSYYATRNGAKFVVVLFDTAADELLAIIEANRLGQIRTGAVTAYATSILHKNVEIFAIIGSGFQAETQLEGMLKVANPTEIRVYSRNQEHARNFSERSSKKFGVSIRAVESAEAALIGADTITTITNSNVPIVYRKYLGDEYHINLAGSNYINRREAESAVLSDANIVVTEHLEQSLIESVEISEYVKLGGKPIELKDVAVDPTRYSKFHRTVFKSMGIGLEDIISGYAVLKNMGII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2026-01-28" version="145" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AJ3</accession>
    <name>CARB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbamoyl phosphate synthase large chain</fullName>
            <ecNumber evidence="1">6.3.4.16</ecNumber>
            <ecNumber evidence="1">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Carbamoyl phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carB</name>
        <name type="ordered locus">TV0817</name>
        <name type="ORF">TVG0823863</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Large subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The large subunit (synthetase) binds the substrates ammonia (free or transferred from glutamine from the small subunit), hydrogencarbonate and ATP and carries out an ATP-coupled ligase reaction, activating hydrogencarbonate by forming carboxy phosphate which reacts with ammonia to form carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <molecule>Carbamoyl phosphate synthase large chain</molecule>
        <reaction evidence="1">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 4 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The large subunit is composed of 2 ATP-grasp domains that are involved in binding the 2 ATP molecules needed for carbamoyl phosphate synthesis. The N-terminal ATP-grasp domain (referred to as the carboxyphosphate synthetic component) catalyzes the ATP-dependent phosphorylation of hydrogencarbonate to carboxyphosphate and the subsequent nucleophilic attack by ammonia to form a carbamate intermediate. The C-terminal ATP-grasp domain (referred to as the carbamoyl phosphate synthetic component) then catalyzes the phosphorylation of carbamate with the second ATP to form the end product carbamoyl phosphate. The reactive and unstable enzyme intermediates are sequentially channeled from one active site to the next through the interior of the protein over a distance of at least 96 A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CarB family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="1"/>
    <dbReference type="EC" id="6.3.5.5" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917061.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AJ3"/>
    <dbReference type="SMR" id="Q97AJ3"/>
    <dbReference type="STRING" id="273116.gene:9381607"/>
    <dbReference type="PaxDb" id="273116-14325033"/>
    <dbReference type="GeneID" id="1441909"/>
    <dbReference type="KEGG" id="tvo:TVG0823863"/>
    <dbReference type="eggNOG" id="arCOG01594">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000513_1_0_2"/>
    <dbReference type="OrthoDB" id="85487at2157"/>
    <dbReference type="PhylomeDB" id="Q97AJ3"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000026">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_A">
        <property type="entry name" value="CPSase_L_chain_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000145087" description="Carbamoyl phosphate synthase large chain">
        <location>
            <begin position="1"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp 1" evidence="1">
        <location>
            <begin position="133"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp 2" evidence="1">
        <location>
            <begin position="665"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="1">
        <location>
            <begin position="911"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carboxyphosphate synthetic domain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Oligomerization domain" evidence="1">
        <location>
            <begin position="399"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate synthetic domain" evidence="1">
        <location>
            <begin position="540"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric domain" evidence="1">
        <location>
            <begin position="916"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
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        </ligand>
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        </ligand>
    </feature>
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        <location>
            <position position="825"/>
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            <name>Mn(2+)</name>
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        <ligand>
            <name>Mn(2+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="827"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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        </ligand>
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            <position position="827"/>
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            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
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        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01210"/>
        </source>
    </evidence>
    <sequence length="1044" mass="116771" checksum="3854A5025AECF6A9" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AN8</accession>
    <name>SYL_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="1">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuS</name>
        <name type="ordered locus">TV0772</name>
        <name type="ORF">TVG0775903</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AN8"/>
    <dbReference type="SMR" id="Q97AN8"/>
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    <dbReference type="PaxDb" id="273116-14324988"/>
    <dbReference type="KEGG" id="tvo:TVG0775903"/>
    <dbReference type="eggNOG" id="arCOG00809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004174_0_0_2"/>
    <dbReference type="OrthoDB" id="23906at2157"/>
    <dbReference type="PhylomeDB" id="Q97AN8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.720">
        <property type="entry name" value="leucyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
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        <property type="entry name" value="aa-tRNA-synth_Ia"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008957">
        <property type="entry name" value="PRK12300.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152149" description="Leucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="611"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="614"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <sequence length="910" mass="104994" checksum="C21870CE07188ED3" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AP3</accession>
    <name>Q97AP3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alanyl-tRNA synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0769525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AP3"/>
    <dbReference type="STRING" id="273116.gene:9381557"/>
    <dbReference type="PaxDb" id="273116-14324983"/>
    <dbReference type="KEGG" id="tvo:TVG0769525"/>
    <dbReference type="eggNOG" id="arCOG01254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_3_2_2"/>
    <dbReference type="OrthoDB" id="11392at2157"/>
    <dbReference type="PhylomeDB" id="Q97AP3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051335">
        <property type="entry name" value="Alanyl-tRNA_Editing_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462:SF1">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN AARSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27386" checksum="D6378AB0F76DF0CE" modified="2001-10-01" version="1">MKTEALYLNDAYKIEGIGTALFVEFTDMKVDKSIFYPTLFGEPNDTGKVIIEGKEYQIVDTIYDGEYIHLISMDTYPQGLVGKSVKQVIDWEKRYVHMRFRTALRLISGLSYKLWGVSCRVNETYDDRAWIDIEKPDITEEEITEVMSKANEMVKQNLEISFSYISMDEFLNRDDLKKMSIHETFDDEKIRIMRVPGLPDQPEFGVNVKNTGEVGEIKYKTTKARGKVSNRININF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AQ0</accession>
    <name>Q97AQ0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0764458</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917006.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AQ0"/>
    <dbReference type="STRING" id="273116.gene:9381549"/>
    <dbReference type="PaxDb" id="273116-14324975"/>
    <dbReference type="GeneID" id="1441854"/>
    <dbReference type="KEGG" id="tvo:TVG0764458"/>
    <dbReference type="eggNOG" id="arCOG00271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978689_0_0_2"/>
    <dbReference type="OrthoDB" id="34518at2157"/>
    <dbReference type="PhylomeDB" id="Q97AQ0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050638">
        <property type="entry name" value="AA-Vitamin_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322:SF18">
        <property type="entry name" value="S-ADENOSYLMETHIONINE_S-ADENOSYLHOMOCYSTEINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="6"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="136"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="280" mass="30856" checksum="1E49FA2E48A15F94" modified="2001-10-01" version="1">MKEIKYLIPYVIFTSFSYYFAKNGVEQVSPSLFMGIRYLLSGLILLPFAKKLRITKNIILLAIMTSTSTAFWAYGLLYVSPAESAVLSYSMPIFSLPIAFLMVSERPSKVEIIGIVIGFTGVIIYGIPLMKGFTIFGAVLTISNAVFWASFTVFYRKLKEEDPFSTNAMQFLIGSLFLFALVPLDPSHNFSLSFAVDVIWMATLGGALQFILWNFMVKISAVNRITVLAFAVPIFTTILGVILSHEIPSGLAIAGVIIMFTGIFVSRLKGGISIVRETSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AS4</accession>
    <name>Q97AS4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0740488 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0740488</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_162009538.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AS4"/>
    <dbReference type="STRING" id="273116.gene:9381524"/>
    <dbReference type="PaxDb" id="273116-14324951"/>
    <dbReference type="GeneID" id="42990950"/>
    <dbReference type="KEGG" id="tvo:TVG0740488"/>
    <dbReference type="HOGENOM" id="CLU_3003372_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="56" mass="6803" checksum="13B1D0CADB9B9460" modified="2001-10-01" version="1">MTKLEDFETYLIGELRMSPSTMKLTMKKWNTRKEDVISMTEHLYRHLLDKDGKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AT2</accession>
    <name>Q97AT2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Integrase / recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0734942</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916981.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AT2"/>
    <dbReference type="STRING" id="273116.gene:9381516"/>
    <dbReference type="PaxDb" id="273116-14324943"/>
    <dbReference type="GeneID" id="1441829"/>
    <dbReference type="KEGG" id="tvo:TVG0734942"/>
    <dbReference type="eggNOG" id="arCOG01241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1286347_0_0_2"/>
    <dbReference type="OrthoDB" id="144892at2157"/>
    <dbReference type="PhylomeDB" id="Q97AT2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00397">
        <property type="entry name" value="DNA_BRE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF41">
        <property type="entry name" value="INTEGRASE_RECOMBINASE PROTEIN MJ0367-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="4">
        <location>
            <begin position="9"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="196" mass="22787" checksum="1B50EB850DF81D23" modified="2001-10-01" version="1">MVKIKDETDLPDHITYDEYQSLINEIINNLYNHYLNSKIIPYLKARDVLLVNCMWELGGRISDILNIEVKDIDFMNKLVILKVKKGGGFINKIPVSDNLLLAFSNFMRELNVSNRRLFKMDRQTAWKKIKGYGNKIGLNLHPHMFRHGLAIYLLLKGVPIQVIARRLGHKNAMTILQYHVVITPYMEREALRGVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AT3</accession>
    <name>Q97AT3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0732760 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0732760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AT3"/>
    <dbReference type="PaxDb" id="273116-14324942"/>
    <dbReference type="DNASU" id="1441828"/>
    <dbReference type="KEGG" id="tvo:TVG0732760"/>
    <dbReference type="eggNOG" id="arCOG07787">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1270000_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13391">
        <property type="entry name" value="HNH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH nuclease" evidence="1">
        <location>
            <begin position="116"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="217" mass="24994" checksum="DAFFBF53FE42D84F" modified="2001-10-01" version="1">MNGMGSKSELEERFSKYNKGEKEPVLCLILEDFVIFEKLVPLSLIDSEGMQVFKYIQDEKMLDTILSAAGVISTSGKEQTLNLDAIDPKFRKAFLNSRLFQGKLRKKALEYYGHRCLLCGIDVDDLLVVSHIKDVFLFPEDAGNLYNTLLLCRLHDGMFDRKLISIGDNNRIIVGSILKNSRSEKLQREVQEMESTTIDRDLKESKKFLEWHRAKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AT8</accession>
    <name>Q97AT8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0729795 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0729795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AT8"/>
    <dbReference type="STRING" id="273116.gene:9381510"/>
    <dbReference type="PaxDb" id="273116-14324937"/>
    <dbReference type="KEGG" id="tvo:TVG0729795"/>
    <dbReference type="HOGENOM" id="CLU_2257517_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="103" mass="11951" checksum="8352891C9857A6F0" modified="2001-10-01" version="1">MISSVIDPILYNFCSKLPGNPIRSFQNVKYILTFIEIVYINYCNFENILSNFNLHPYLDIFSSYTFLLILIIIVRSLIKDQFNDDVRTLSERISISTAVSPYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AV4</accession>
    <name>Q97AV4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0710523</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916964.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AV4"/>
    <dbReference type="STRING" id="273116.gene:9381494"/>
    <dbReference type="PaxDb" id="273116-14324921"/>
    <dbReference type="GeneID" id="1441812"/>
    <dbReference type="KEGG" id="tvo:TVG0710523"/>
    <dbReference type="eggNOG" id="arCOG01137">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_2_2_2"/>
    <dbReference type="OrthoDB" id="56956at2157"/>
    <dbReference type="PhylomeDB" id="Q97AV4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050563">
        <property type="entry name" value="4-hydroxybenzoyl-CoA_TE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006684">
        <property type="entry name" value="YbgC/YbaW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793">
        <property type="entry name" value="4-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793:SF24">
        <property type="entry name" value="LONG-CHAIN ACYL-COA THIOESTERASE FADM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003230">
        <property type="entry name" value="YbgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15439" checksum="B7C63C0935CEFD4E" modified="2001-10-01" version="1">MVESAYSKTKIQVRYNDLDTVGHVNNAIYFTYFEIGRIDFINKFLWKFKSDEINFVIARAEADFIKSILLYDVVYVRTWISRVGNTSFDFSYRLEDEDGKEYATGKTINVILSNGKPSPVPNEIKALLMENEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AX3</accession>
    <name>Q97AX3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0691625 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0691625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916944.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AX3"/>
    <dbReference type="STRING" id="273116.gene:9381474"/>
    <dbReference type="PaxDb" id="273116-14324902"/>
    <dbReference type="GeneID" id="1441792"/>
    <dbReference type="KEGG" id="tvo:TVG0691625"/>
    <dbReference type="eggNOG" id="arCOG04913">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2178030_0_0_2"/>
    <dbReference type="OrthoDB" id="141013at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="109" mass="12918" checksum="67F990A0F1CE2DAD" modified="2001-10-01" version="1">MDECQLYSVLLELKLPWKVGRVSLDSMKKTVEIYITHEKSSKLLCPMCRKECMVYDHLRERVWRDLDSIEFMSFVHANPQSPKDILPGTWRHRSRIAMGREDIKAHSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B40</accession>
    <name>Q97B40_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphomannomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG0612677</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916878.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B40"/>
    <dbReference type="STRING" id="273116.gene:9381407"/>
    <dbReference type="PaxDb" id="273116-14324835"/>
    <dbReference type="GeneID" id="1441726"/>
    <dbReference type="KEGG" id="tvo:TVG0612677"/>
    <dbReference type="eggNOG" id="arCOG00767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="PhylomeDB" id="Q97B40"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000001">
        <property type="entry name" value="Phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050060">
        <property type="entry name" value="Phosphoglucosamine_mutase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE (ALPHA-D-GLUCOSE-1,6-BISPHOSPHATE-DEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946">
        <property type="entry name" value="PHOSPHOHEXOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="5"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="152"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="256"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="8">
        <location>
            <begin position="367"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB59761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="447" mass="48810" checksum="D6FE88BB6F3AE0E5" modified="2001-10-01" version="1">MAVERLFGTNGIRGIPNDDMSPDFAMSVGKAIGTYVSGEIAFGTDTRLTGDMIKNAVIAGILSTGISVIDLGVLPTPAIQYYSKKHGIYAVVITASHNPPQYNGIKVIDKDGTELERPGEEKIEEIFYSKAFRVVKWENIGRIRQVSDAIDIYINGVISNVDSDNIKSHAFKVLADAGNGAAYYSTPKLLEKLGCSITTLNANPDGRFTARNAEPVPENLVNLISLMKSGRFDLGIAHDGDADRAVFIDEKGNFIDGDRMLALITKYIAKAGDVVVTPVSSSDTIADICNAIGAKLIRTKVGAPIVARAMIDNGATIGGEENGGVIYGKHQYCRDGAMTVSLVLNLMASTNKKLSELISELPVYYLIKKKVERRVDWPSLARELKKGERVDETDGIKILKDDGWILIRPSGTEKILRIYAHSSDFELANKYCEQYIRKVNDIQDKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B61</accession>
    <name>Q97B61_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0590237 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0590237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_156769058.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B61"/>
    <dbReference type="STRING" id="273116.gene:9381385"/>
    <dbReference type="PaxDb" id="273116-14324812"/>
    <dbReference type="GeneID" id="1441703"/>
    <dbReference type="KEGG" id="tvo:TVG0590237"/>
    <dbReference type="eggNOG" id="arCOG07383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_277279_0_0_2"/>
    <dbReference type="OrthoDB" id="57511at2157"/>
    <dbReference type="PhylomeDB" id="Q97B61"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1094"/>
            <end position="1112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="1144" mass="129227" checksum="2AFB1F9F7A0EBA70" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B82</accession>
    <name>Q97B82_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Indolpyruvate ferredoxin oxidoreductase [IOR] beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0563052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916834.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B82"/>
    <dbReference type="STRING" id="273116.gene:9381361"/>
    <dbReference type="PaxDb" id="273116-14324790"/>
    <dbReference type="GeneID" id="1441092"/>
    <dbReference type="KEGG" id="tvo:TVG0563052"/>
    <dbReference type="eggNOG" id="arCOG01602">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q97B82"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052198">
        <property type="entry name" value="IorB_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005328">
        <property type="entry name" value="PRK06853.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43854">
        <property type="entry name" value="INDOLEPYRUVATE OXIDOREDUCTASE SUBUNIT IORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43854:SF1">
        <property type="entry name" value="INDOLEPYRUVATE OXIDOREDUCTASE SUBUNIT IORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="2">
        <location>
            <begin position="12"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="194" mass="20797" checksum="6BA0DEDD38E53647" modified="2001-10-01" version="1">MESLNLLIAGVGGQGIITMGKMLATAADLAGVKVIVSETHGLSQRGGSVAVHVRMGNVYAPLIERGKADYIIGLEASEAVRNLSYAGPNTKFIINKNLIKPVLPKVMPATLDEVFEALKDYQVYHIDAETIAAESGNRKGSNAVLLGYMYSINAFRTLKISVNNFLQAMVSESNKACFRAGIGVNRLTDLTNSH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BB3</accession>
    <name>Y544_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized solute-binding protein TV0544</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0544</name>
        <name type="ORF">TVG0535415</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein 1 family. WtpA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916802.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BB3"/>
    <dbReference type="SMR" id="Q97BB3"/>
    <dbReference type="STRING" id="273116.gene:9381329"/>
    <dbReference type="PaxDb" id="273116-14324759"/>
    <dbReference type="GeneID" id="1441060"/>
    <dbReference type="KEGG" id="tvo:TVG0535415"/>
    <dbReference type="eggNOG" id="arCOG00219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_709076_0_0_2"/>
    <dbReference type="OrthoDB" id="56205at2157"/>
    <dbReference type="PhylomeDB" id="Q97BB3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030973">
        <property type="term" value="F:molybdate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13540">
        <property type="entry name" value="PBP2_ModA_WtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050682">
        <property type="entry name" value="ModA/WtpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632">
        <property type="entry name" value="MOLYBDATE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632:SF16">
        <property type="entry name" value="MOLYBDATE_TUNGSTATE-BINDING PROTEIN WTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13531">
        <property type="entry name" value="SBP_bac_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000159729" description="Uncharacterized solute-binding protein TV0544">
        <location>
            <begin position="30"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="389" mass="42944" checksum="D60EBB4F1EA5E0B5" modified="2001-10-01" version="1" precursor="true">MQPSFTPSGGKWLSIAVILLVIGLVVGFAAGRFTAPIQQSKELNTFAAGSLKYALGNDFNPEFNNLTGIKVGMTFSGSISGAKEVQAGKQYSVFVSASAPVLYQNLINNTHYASWQIVFSANEMAITWTNSKYAINPSWPYWFENITKNSTIVAASNASLDPSGFQAIETMKLAGILYTNWSDPFVQMAFNHNESLFLQYNKAYNTWFEKLGYKANDSLALYHQIFISKYLNHTTKLTTVEIGLDGYLTSGAADYALTYVSQAINQNLSYYKSATGGNGLPIWINLGSLNKTIDQFYEQINESGPAWDNVGNLPGAPILYSVTIINNYTSPYAVQYVYDLITQMGQHYLAMSRFDPLSQPFGINISNMPKQLQAVVTPPPSYLPVSAYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BB5</accession>
    <name>Q97BB5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="3">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0533545</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916800.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BB5"/>
    <dbReference type="STRING" id="273116.gene:9381327"/>
    <dbReference type="PaxDb" id="273116-14324757"/>
    <dbReference type="GeneID" id="1441058"/>
    <dbReference type="KEGG" id="tvo:TVG0533545"/>
    <dbReference type="eggNOG" id="arCOG04367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002977_4_1_2"/>
    <dbReference type="OrthoDB" id="371943at2157"/>
    <dbReference type="PhylomeDB" id="Q97BB5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009330">
        <property type="term" value="C:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.268.10:FF:000001">
        <property type="entry name" value="DNA gyrase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000001">
        <property type="entry name" value="DNA gyrase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="entry name" value="DNA gyrase/topoisomerase IV, subunit A, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050220">
        <property type="entry name" value="Type_II_DNA_Topoisomerases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01063">
        <property type="entry name" value="gyrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004043">
        <property type="entry name" value="PRK05560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004044">
        <property type="entry name" value="PRK05561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43493:SF5">
        <property type="entry name" value="DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43493">
        <property type="entry name" value="DNA GYRASE/TOPOISOMERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="GyrA/ParC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="8">
        <location>
            <begin position="27"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="425"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="792" mass="89039" checksum="6EE0A2C5E934D585" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="138" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BB6</accession>
    <name>Q97BB6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="4">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">TVG0531155</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type II topoisomerase GyrB family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916799.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BB6"/>
    <dbReference type="STRING" id="273116.gene:9381326"/>
    <dbReference type="PaxDb" id="273116-14324756"/>
    <dbReference type="GeneID" id="1441057"/>
    <dbReference type="KEGG" id="tvo:TVG0531155"/>
    <dbReference type="eggNOG" id="arCOG04371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006146_4_1_2"/>
    <dbReference type="OrthoDB" id="358756at2157"/>
    <dbReference type="PhylomeDB" id="Q97BB6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16928">
        <property type="entry name" value="HATPase_GyrB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00822">
        <property type="entry name" value="TopoII_Trans_DNA_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03366">
        <property type="entry name" value="TOPRIM_TopoIIA_GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000002">
        <property type="entry name" value="DNA gyrase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000002">
        <property type="entry name" value="DNA gyrase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002288">
        <property type="entry name" value="DNA_gyrase_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000565">
        <property type="entry name" value="Topo_IIA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034160">
        <property type="entry name" value="TOPRIM_GyrB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004189">
        <property type="entry name" value="PRK05644.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45866:SF1">
        <property type="entry name" value="DNA GYRASE SUBUNIT B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45866">
        <property type="entry name" value="DNA GYRASE/TOPOISOMERASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00986">
        <property type="entry name" value="DNA_gyraseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01159">
        <property type="entry name" value="DNAGYRASEB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="12">
        <location>
            <begin position="427"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAB59683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="641" mass="71575" checksum="8FDEFA13C5E2EB4D" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-20" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BC5</accession>
    <name>PFDA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Prefoldin subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GimC subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pfdA</name>
        <name type="ordered locus">TV0531</name>
        <name type="ORF">TVG0522388</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916789.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BC5"/>
    <dbReference type="SMR" id="Q97BC5"/>
    <dbReference type="STRING" id="273116.gene:9381315"/>
    <dbReference type="PaxDb" id="273116-14324746"/>
    <dbReference type="GeneID" id="1441047"/>
    <dbReference type="KEGG" id="tvo:TVG0522388"/>
    <dbReference type="eggNOG" id="arCOG01341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156999_0_0_2"/>
    <dbReference type="OrthoDB" id="56120at2157"/>
    <dbReference type="PhylomeDB" id="Q97BC5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000153691" description="Prefoldin subunit alpha">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="130" mass="14618" checksum="6542126CADCFA1ED" modified="2001-10-01" version="1">MARDVEAQLNYIESLISSVDAQIDAINKMVLEVQTTIQILSSDELRQSKERLISVGSGIFANGNLDLDSDLIVPIGSGVYIAEKRSETIERLKANIENLKESIQKLMDQRRTLVDQYNTVYTTEATRNIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BD7</accession>
    <name>RIFK_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Riboflavin kinase</fullName>
            <shortName>RFK</shortName>
            <ecNumber>2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ribK</name>
        <name type="ordered locus">TV0519</name>
        <name type="ORF">TVG0510159</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>riboflavin + CTP = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916777.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BD7"/>
    <dbReference type="SMR" id="Q97BD7"/>
    <dbReference type="STRING" id="273116.gene:9381303"/>
    <dbReference type="PaxDb" id="273116-14324734"/>
    <dbReference type="GeneID" id="1441035"/>
    <dbReference type="KEGG" id="tvo:TVG0510159"/>
    <dbReference type="eggNOG" id="arCOG01904">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088476_0_0_2"/>
    <dbReference type="OrthoDB" id="30955at2157"/>
    <dbReference type="PhylomeDB" id="Q97BD7"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010762">
        <property type="entry name" value="PRK14165.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706:SF1">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000322108" description="Riboflavin kinase">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="H-T-H motif-like">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Riboflavin kinase">
        <location>
            <begin position="93"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="102"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="200"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="220" mass="24808" checksum="3ED2D5F033A08FE3" modified="2001-10-01" version="1">MDTSDQYYRAIKKIKEASDSSNKAYLTSSKLANMLGLSQQSASRIIIDLEKLGYITRTVSKRGQLLTITEKGLDLLYTEFAELSRILSIKSNIVMTGIVVPGMGEGKYYISRKQYIIQFQEKLGIIPYLGTLNIKVDPSSIPELRKLRGFTGIHIEGFRTEDRTFGSVKAFRCKTNGVPSFLIMPERTVYTDVVEIISDKYLRNELNLKDGDEVTIEVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BH5</accession>
    <name>Q97BH5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0468499 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0468499</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916739.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BH5"/>
    <dbReference type="STRING" id="273116.gene:9381262"/>
    <dbReference type="PaxDb" id="273116-14324695"/>
    <dbReference type="GeneID" id="1440997"/>
    <dbReference type="KEGG" id="tvo:TVG0468499"/>
    <dbReference type="eggNOG" id="arCOG00317">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313887_0_0_2"/>
    <dbReference type="OrthoDB" id="57156at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018686">
        <property type="entry name" value="DUF2175"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09943">
        <property type="entry name" value="DUF2175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="100" mass="11388" checksum="42D72C7BE085B169" modified="2001-10-01" version="1">MAEYKCYVCEKPVKTGEKFTFTKKGSVHYDCFIADKRNKLGEEKLENLRVLSILLDSNLQNLVNVLGVPADSPELAEIKRQNIQSIEKMAGDITRKIEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BI1</accession>
    <name>Q97BI1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0463720</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916734.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BI1"/>
    <dbReference type="STRING" id="273116.gene:9381256"/>
    <dbReference type="PaxDb" id="273116-14324689"/>
    <dbReference type="GeneID" id="1440992"/>
    <dbReference type="KEGG" id="tvo:TVG0463720"/>
    <dbReference type="eggNOG" id="arCOG05331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_591375_0_0_2"/>
    <dbReference type="OrthoDB" id="42259at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="402" mass="44288" checksum="0AB11E046A940D19" modified="2001-10-01" version="1">MISVTAYEWIGLFIGMIIGGLAEAWGISNPEVLIRLGKWEDRLFIGCIAIAIGAGAIVLYGLSALGVSFHWGIKPDYVIGVALGGIIFGTGIAISGYVPGTVWMALGEGRRDAIYAVLGGLLGAASWTLIYQTSFGHWLTTYLNFGQIYFGGKVGTDLKLGFIIAIVWAIIMFVIAYTLPRYRKGKSCAYLSTHKNYKLDAEEEKEYIETANFLMEGTSMPIGKNNLPEKVNYHWTVSSKLFGVTMLSVGIIIGLTVVLEMFLHQIFGESTTYSWIVAQIWLPAGYWQYSTIVVHTIGWEPFSDIGTLFGAFLASIFFTRRFQAFSNVIPPSWKERFGNSQIKRAAGAFSGAYLMLLGARMADGCASGHILSGDLQMAVSSLEFFIFVVIFLLLVAHIIYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BI5</accession>
    <name>Q97BI5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0459657</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916730.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BI5"/>
    <dbReference type="STRING" id="273116.gene:9381252"/>
    <dbReference type="PaxDb" id="273116-14324685"/>
    <dbReference type="GeneID" id="1440988"/>
    <dbReference type="KEGG" id="tvo:TVG0459657"/>
    <dbReference type="eggNOG" id="arCOG05331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_591375_0_0_2"/>
    <dbReference type="OrthoDB" id="42259at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="465" mass="51401" checksum="2239F90146F4B2B0" modified="2001-10-01" version="1">MNENIGSINISSDAYKRDKSLFWKSVYALLILALALLIGGIIMQVLFHVTKAPLLLGLIVAFPLAIPLELWNFTDPDTLFRVMAFQDRFLMVCFGFAIGLGSIILYLVAIFTHPNFGIKDFFVPGIVIGGLIFGIGVGLAGYFPGTIWIALGQGRRDAIYASFGGLLGAFTWSIIFGNVKWFFWDTLNFGPITWASVAGISSKFGVFAVSLVFGIILLLVFLLLPRYPKSKVKDSCGYHMLGKSEKTITFDLVISEDFEKYPKANRYLTKLINESSKENARMTIPLGLAFTITAVAVIILHQIFGESTTFSWIGAQLSYLANPTWAASNAYFQLFGGLHMVNGVPVNHPFSEIGWEPFTDLSSFLGGLIAATLISKRYMAFKPQVPKIWSHSHNPKYRPIGAFLGAFMVLFGARMANGCASGHILSGNIQMAISSFVFMIFVLLGAWVTLRYFMHLKINSQGYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BJ1</accession>
    <name>Q97BJ1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type I restriction enzyme S protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0452892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BJ1"/>
    <dbReference type="REBASE" id="685113">
        <property type="entry name" value="S.TvoORF2430P"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324679"/>
    <dbReference type="KEGG" id="tvo:TVG0452892"/>
    <dbReference type="eggNOG" id="arCOG02626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519966_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1120">
        <property type="entry name" value="Bipartite methylase S protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="entry name" value="DNA methylase specificity domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116734">
        <property type="entry name" value="DNA methylase specificity domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0680">Restriction system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="84" mass="9600" checksum="6C63D44B64FF0003" modified="2001-10-01" version="1">MPMKDSGIEWIGSINSKWPIVKIIYFSKLKTCGTPDKRVLEYWEDGKINWMSSGEINKDLIYEVEGKITELGYKNSNATSLPVN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="139" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BJ8</accession>
    <name>G3P_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <shortName evidence="2">GAPDH</shortName>
            <ecNumber evidence="2">1.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gap</name>
        <name type="ordered locus">TV0457</name>
        <name type="ORF">TVG0444310</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = (2R)-3-phospho-glyceroyl phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.59" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_010916716.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
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    <dbReference type="AlphaFoldDB" id="Q97BJ8"/>
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    <dbReference type="STRING" id="273116.gene:9381238"/>
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    <dbReference type="GeneID" id="1440974"/>
    <dbReference type="KEGG" id="tvo:TVG0444310"/>
    <dbReference type="eggNOG" id="arCOG00493">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_069533_0_0_2"/>
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        <property type="reaction ID" value="UER00184"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
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        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
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        <property type="entry name" value="GAPDH_II_C"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DapB_N"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
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        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="NCBIfam" id="TIGR01546">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK04207.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00846">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145738" description="Glyceraldehyde-3-phosphate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
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    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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    <feature type="binding site" evidence="2">
        <location>
            <position position="109"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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    <feature type="binding site" evidence="2">
        <location>
            <begin position="138"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
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        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="192"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00559"/>
        </source>
    </evidence>
    <sequence length="338" mass="37374" checksum="C9BD53DFD4722F34" modified="2001-10-01" version="1">MIRVGINGYGTIGRRVANAVSSQDDMIVVGIVKTKPDYISEVASRRFKIFVPDSSYMKAFQDAGIKVEGTLDNLLDDAEIIVDATPEGMGEKNKPLYIKKKAKAIFEGGEEPDVAETSFNAYSNYNDAIGKSYVRVVSCNTTGLARTLYPIQQAFGVKHVEATLIRRATDQNDSSKGPINAVEPSLKIPSHHAPDLKTVMGNIDVNTVAIKVPTTLMHVHVVQVDTEKNASNDGVLEAWNNYRRIIHVKKDDGIKSTAQIMDLAREFGRDRSDLYEIAIWEGSVSAKANRISYIQAVHQESDVIPENVDAIRAMFNLADKEKSIEKTDKSLGIEKRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BP7</accession>
    <name>Q97BP7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7839 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0396051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43704">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43704:SF2">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="178"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="264" mass="29827" checksum="6187F52D77BB0FA9" modified="2001-10-01" version="1">MQILYYAFDIHNMLPKPDQMNQVIVLLNVYHGRRKPSEISKNMDITLQGVIYHLKNLRKSRYIDEENRITKEGFQFLYDQLSEMREFINENITDLDSVMVWEAIAGSRIEKGDDIFLRMNSGYLYAYHEGPGASGKAENDASEGDLVGVTQISGMIDVSIGVLDIVIIPDSLDETVISKLKGIIEDRKNSEIAAMGEGGFVAARKLGLNPSIEFGSLPASFEACVRGISVILLVSRRRFHYSLREIPELENRYNPVRAHIIDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BR1</accession>
    <name>Q97BR1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0380863 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0380863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916649.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BR1"/>
    <dbReference type="PaxDb" id="273116-14324609"/>
    <dbReference type="GeneID" id="1440907"/>
    <dbReference type="KEGG" id="tvo:TVG0380863"/>
    <dbReference type="eggNOG" id="arCOG05404">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2103361_0_0_2"/>
    <dbReference type="OrthoDB" id="57108at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="114" mass="13350" checksum="B806867442504F97" modified="2001-10-01" version="1">MADSEQELFLCRCRHKAVKDIAKKIGVKKAYLEKLILKNIADTDSIMQRLVEGRTVKKLNPDISPIIRQYLEGNISAEELLRNDDVLDYIAVKIKDHHDRYMDCIRFIYKNITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BR2</accession>
    <name>Q97BR2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0380506</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916648.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BR2"/>
    <dbReference type="STRING" id="273116.gene:9381170"/>
    <dbReference type="PaxDb" id="273116-14324608"/>
    <dbReference type="GeneID" id="1440906"/>
    <dbReference type="KEGG" id="tvo:TVG0380506"/>
    <dbReference type="eggNOG" id="arCOG01622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_4_2"/>
    <dbReference type="OrthoDB" id="25239at2157"/>
    <dbReference type="PhylomeDB" id="Q97BR2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="2">
        <location>
            <begin position="126"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="321" mass="36074" checksum="9167F662F77898C7" modified="2001-10-01" version="1">MKVNIIGLNGFGKVHLEAWSKTDVDIEITERKKEIVEELKQKYNISKVYGSLEEALRSDAEIFDIVLPHSMHRDVAVKSLESGKHVLIEKPIATEVRDAQIMISTAEKNKRKFMVADQYFFDPSVKKIIDIIKAGDIGKVHSIIIRDQRKYNWHGWRAEAEHMGGGSLIDGGIHFIDTLLNIGGDYSAVRSLSYKSTQSIQEPDTVEAIFKFKNGSNGMFFYSWGYPFTPKLPAYEVIGETGSIVEDVVSKPVGFDAPKGKRAYGDPIVNAKLIDLGEHDIFYDEVSGFLKSVVKNENVPFDPYLALRDLVAVKDIYANQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BS9</accession>
    <name>Q97BS9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rieske iron sulfur protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0366346</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916630.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BS9"/>
    <dbReference type="STRING" id="273116.gene:9381153"/>
    <dbReference type="PaxDb" id="273116-14324591"/>
    <dbReference type="GeneID" id="1440888"/>
    <dbReference type="KEGG" id="tvo:TVG0366346"/>
    <dbReference type="eggNOG" id="arCOG01720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074282_0_0_2"/>
    <dbReference type="OrthoDB" id="5623at2157"/>
    <dbReference type="PhylomeDB" id="Q97BS9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="122"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="279" mass="30651" checksum="0056B470B470CB07" modified="2001-10-01" version="1">MANTQSEDEPKDRLRRSFLKGAAVSLFSLALGSLSIEKFFVPTTRNVPVSSYNGKYPTAILVDPDGTPIKASEIPYALPASSSYPILVFNYPLQDEPNILIRLRNVSIPNAIHINNSTGGVDSITAYSGICQHLGCTVPLLDYHPGNSIPFEAQLIGYTKKNWPEYGLLYCKCHGSQYDPIRGPHNLYNSGPAPSPANHSLPQVLFYHDSATDYLYAIGMNPVNAVIRTHLWQPNGETYGPEVVDENLSGGTELPFDNTLGHYKATVVSSSNFKWSGRF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BV5</accession>
    <name>RL30_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl30</name>
        <name type="ordered locus">TV0350</name>
        <name type="ORF">TVG0343183</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916604.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BV5"/>
    <dbReference type="SMR" id="Q97BV5"/>
    <dbReference type="STRING" id="273116.gene:9381127"/>
    <dbReference type="PaxDb" id="273116-14324565"/>
    <dbReference type="GeneID" id="1440862"/>
    <dbReference type="KEGG" id="tvo:TVG0343183"/>
    <dbReference type="eggNOG" id="arCOG04086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055156_6_0_2"/>
    <dbReference type="OrthoDB" id="6379at2157"/>
    <dbReference type="PhylomeDB" id="Q97BV5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_A">
        <property type="entry name" value="Ribosomal_uL30_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005997">
        <property type="entry name" value="Ribosomal_uL30_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004711">
        <property type="entry name" value="PRK06049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01309">
        <property type="entry name" value="uL30_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000273917" description="Large ribosomal subunit protein uL30">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="165" mass="18781" checksum="86D36B80F2DC2675" modified="2001-10-01" version="1">MLAVIRIRGRTGIKQDIEDTAHLLRLNRINHLVLLQEDAVTKGMLQKVKDYVTWGEIDVDTLEVLLKNRCLFKGRRKLTEEELKDVTGFGSYRDLAKALVDGKIKFSEINDVVPVIRLNPPYKGYEAIKTSYRNGGSAGYRGKDINNLIRRMIIPGVDLNGQREN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BW6</accession>
    <name>RL14_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl14</name>
        <name type="ordered locus">TV0339</name>
        <name type="ORF">TVG0337944</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L24e, part of which may contact the 16S rRNA in 2 intersubunit bridges.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916593.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BW6"/>
    <dbReference type="SMR" id="Q97BW6"/>
    <dbReference type="STRING" id="273116.gene:9381116"/>
    <dbReference type="PaxDb" id="273116-14324554"/>
    <dbReference type="GeneID" id="1440851"/>
    <dbReference type="KEGG" id="tvo:TVG0337944"/>
    <dbReference type="eggNOG" id="arCOG04095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_3_0_2"/>
    <dbReference type="OrthoDB" id="23569at2157"/>
    <dbReference type="PhylomeDB" id="Q97BW6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000007">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019971">
        <property type="entry name" value="Ribosomal_uL14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006344">
        <property type="entry name" value="PRK08571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03673">
        <property type="entry name" value="uL14_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000266617" description="Large ribosomal subunit protein uL14">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="132" mass="14196" checksum="A02F32293BCEA515" modified="2001-10-01" version="1">MKGIAGREIRGLPLGANIVCADNTGARSISLIDVKAYHGKARRIPAAGVGDMFIASVKKGTPEMRSKVVYAVVIRQKRPYRRPDGTMVEFEDNAAVLVTPDGEVRGSEIKGPVAREAAERWPRIAAIASIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BY4</accession>
    <name>Q97BY4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0326903 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0326903</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916576.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BY4"/>
    <dbReference type="STRING" id="273116.gene:9381097"/>
    <dbReference type="PaxDb" id="273116-14324536"/>
    <dbReference type="GeneID" id="1440834"/>
    <dbReference type="KEGG" id="tvo:TVG0326903"/>
    <dbReference type="eggNOG" id="arCOG05357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1954524_0_0_2"/>
    <dbReference type="OrthoDB" id="52916at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="127" mass="15498" checksum="FF00B359D61DAEC7" modified="2001-10-01" version="1">MKNWSGHIYFDPKFEWSKLDKAFQEIFEHIVEQKKNPEDLQFDQVILQLNLEEIKKNKKPIGYIKDDARYKLVFPLDRKEMIIYRGVVSEDVHEKTEEVEKILKNKKIKYTVDYDKMLLYEIKRSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C03</accession>
    <name>Q97C03_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molybdopterin cofactor biosynthetic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0312436</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916557.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C03"/>
    <dbReference type="STRING" id="273116.gene:9381076"/>
    <dbReference type="PaxDb" id="273116-14324517"/>
    <dbReference type="GeneID" id="1440815"/>
    <dbReference type="KEGG" id="tvo:TVG0312436"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_5_2"/>
    <dbReference type="OrthoDB" id="5620at2157"/>
    <dbReference type="PhylomeDB" id="Q97C03"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022563">
        <property type="entry name" value="DUF3463"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023863">
        <property type="entry name" value="rSAM_PTO1314"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03961">
        <property type="entry name" value="rSAM_PTO1314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF7">
        <property type="entry name" value="PQQA PEPTIDE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11946">
        <property type="entry name" value="DUF3463"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="22"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="336" mass="38509" checksum="E78A53385338C1C3" modified="2001-10-01" version="1">MAIIEPIIGRSIKRKMGGMRGKKMPFIAGHKLLYTCNLRCDMCPFWRRKDERLLSIDEEIMMMDSLASAGVSFMGFEGGEPLLRNDLEDILRESHRRFHTSLVTNGWLLKNRASSIAKYLDYLFVSIDGLKETHDKQRGVPGSFEHALEGIEEAKKYVQVAISTTITTYNMNEITKVVELAKDLGVGINVQVSYDYSTANNLSPEKKALYDVLLSLKKMKEDGYPIVNSKEYFDTLISSWYYGIKWACKPWSTVNIDPQGRIVMPCYVLNEYAGSSKVWETDIVRLWNTYDWSQYESCNKCSLSCYLEPSIFSWKKLSMVNERIIGSIANYISSML</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C11</accession>
    <name>Y294_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative HTH-type transcriptional regulatory protein TV0294</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0294</name>
        <name type="ORF">TVG0304564</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59436" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59436.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916549.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C11"/>
    <dbReference type="SMR" id="Q97C11"/>
    <dbReference type="STRING" id="273116.gene:9381068"/>
    <dbReference type="PaxDb" id="273116-14324509"/>
    <dbReference type="GeneID" id="1440807"/>
    <dbReference type="KEGG" id="tvo:TVG0304564"/>
    <dbReference type="eggNOG" id="arCOG04152">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075726_0_0_2"/>
    <dbReference type="OrthoDB" id="31424at2157"/>
    <dbReference type="PhylomeDB" id="Q97C11"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00584">
        <property type="entry name" value="HTH_type_cro_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020886">
        <property type="entry name" value="MTH_967-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059051">
        <property type="entry name" value="MTH_967_PDDEXK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003162">
        <property type="entry name" value="PRK04140.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26553">
        <property type="entry name" value="PDDEXK_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000144869" description="Putative HTH-type transcriptional regulatory protein TV0294">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="133"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="312" mass="35172" checksum="78C990253BFA1C84" modified="2002-10-25" version="2">MEDRREALISKLMNMLVENGFSVSGTGFQPVATFDIVARRDVERYILKVLYNIDTLKQSTAYQLIKLAKLLRATAAIIGERTGNGQLEDGVVYYRHGVPISSVNTFESYLNGERPYIYSGPGGFYVRINGELLRERRNELNLSIGNISSYLGVSRRSVSLYENGSAATIDIFIRLRNILKADIVDHIDLFRINSTEINFEEKISDEYIARIIWLLQKYGLDTRPIFKVPFDIVAKDSDEISLIAALIGDAPEPERVRILKKISDVFEDDAFLVSKSNTYRESIGGCAVLTISDLESIEDKDALIGKIEKRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C21</accession>
    <name>Q97C21_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,4-dihydroxy-6-naphtoate synthase</fullName>
            <ecNumber evidence="3">4.1.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Menaquinone biosynthetic enzyme MqnD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mqnD</name>
        <name evidence="4" type="ORF">TVG0294833</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of cyclic dehypoxanthine futalosine (cyclic DHFL) into 1,4-dihydroxy-6-naphthoate, a step in the biosynthesis of menaquinone (MK, vitamin K2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cyclic dehypoxanthinylfutalosinate = 1,4-dihydroxy-6-naphthoate + dihydroxyacetone</text>
            <dbReference type="Rhea" id="RHEA:33087"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:64254"/>
            <dbReference type="ChEBI" id="CHEBI:64270"/>
            <dbReference type="EC" id="4.1.99.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MqnA/MqnD family. MqnD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.29" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C21"/>
    <dbReference type="SMR" id="Q97C21"/>
    <dbReference type="STRING" id="273116.gene:9381058"/>
    <dbReference type="PaxDb" id="273116-14324498"/>
    <dbReference type="KEGG" id="tvo:TVG0294833"/>
    <dbReference type="eggNOG" id="arCOG00654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070326_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97C21"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00996">
        <property type="entry name" value="MqnD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003773">
        <property type="entry name" value="Menaquinone_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030869">
        <property type="entry name" value="MqnD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37167">
        <property type="entry name" value="1,4-DIHYDROXY-6-NAPHTOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37167:SF1">
        <property type="entry name" value="1,4-DIHYDROXY-6-NAPHTOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02621">
        <property type="entry name" value="VitK2_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 3" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="277" mass="31886" checksum="2E84DE6C62B91C87" modified="2001-10-01" version="1">MNIFVNMRIMVAHTPDPDDAFMFYGMFEGKIRTSNQYEQVIKDIETLNKESKEGKYEVTAISANGYVQVRDLYKLTRSGASFGISYGPIVVARNEIDLSKSRMGVPGFMTSSYLLYRMFGPEAKEYVEIKFDEIPDAILTGKVDAGILIHDEQLTFQKRGLKEVFDIYSAWKSYAGNLPIPLGFNAIRKDLDEDTVNKFLYDFERSIRYAMAHEEEAVKYAMKYARYTDLDLERRFVRMYVNELSIDFGDTGRKALELYYNRAAGKGLIPPFKPEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C37</accession>
    <name>Q97C37_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0279375 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0279375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C37"/>
    <dbReference type="PaxDb" id="273116-14324482"/>
    <dbReference type="KEGG" id="tvo:TVG0279375"/>
    <dbReference type="eggNOG" id="arCOG05382">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507465_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="178" mass="20280" checksum="AF21699725CBA335" modified="2001-10-01" version="1">MITVMFFMLVETAQPYYSAYIVIGILALLLLRSAYRSFKGRRYRPARVFRIPAVYALLTVILVLLDFSTQYYYYSVLLLIIAGYLVGVRVGTSVRFFYRGTVLYYRRSPGVYVIWAVSLFSRVILEFLMPGNSLAEVSVDSILSFTTGLLIGETVNIIKQKRVFDEKIGDTTQNSSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C48</accession>
    <name>Q97C48_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0270671</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916514.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C48"/>
    <dbReference type="STRING" id="273116.gene:9381029"/>
    <dbReference type="PaxDb" id="273116-14324471"/>
    <dbReference type="GeneID" id="1440772"/>
    <dbReference type="KEGG" id="tvo:TVG0270671"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_60_4_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="PhylomeDB" id="Q97C48"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17329">
        <property type="entry name" value="MFS_MdtH_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517:SF14">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="15"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="403" mass="44743" checksum="1EB5C0332DF6267A" modified="2001-10-01" version="1">MAVLSSKDLMFENRTLWGVSTAAAVRSIGYGATWPFMAVYFNEYLHLSLIFVGFIFTLNAALSIVFSLFAGYLSDVIGRKWTLISGSVAGFILYALLALLVYRAVLIASALFVMTAFSGSLVYPSANAIVSDVTTEKNREMGYTVYRIMANLGWAVGPLISSFIFRYGFEFIFLFVAVANLLSTIVSLLYVKRNVRYREAKRNFFVVDYRLYLFSIPVFLLIMVSSQFSVTLPIFVVEDLHIVVSNLGYFYAVNGTVVVIGQYPISKLMSRYPDIYALVFGAIFYSIGYLMVSFSRNLPSLIIDMIIITIGENLTSPNINTVVSKIAPKESVGQFMGFIGMVNQLARAAAPSVGTLILYSLGQRPGLVWPVIDIFGISAAILFFSFRGLFVPRKRIRGAAMQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C61</accession>
    <name>Q97C61_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0257960 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0257960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_083755821.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C61"/>
    <dbReference type="STRING" id="273116.gene:9381015"/>
    <dbReference type="PaxDb" id="273116-14324458"/>
    <dbReference type="GeneID" id="1440760"/>
    <dbReference type="KEGG" id="tvo:TVG0257960"/>
    <dbReference type="eggNOG" id="arCOG07741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243999_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012440">
        <property type="entry name" value="DUF1641"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07849">
        <property type="entry name" value="DUF1641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="104" mass="11556" checksum="98FFC71B53202D5A" modified="2001-10-01" version="1">MIKAIMEHKADVVGVATEEIYKEKNQNFLRNILTIYTLLSSIDSERLRKFMENAAKSIESADKLKAEGSLSLLKIGSQLKDPDVSAGVRVLLSVAKGFTKDEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C83</accession>
    <name>Q97C83_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0228061</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C83"/>
    <dbReference type="STRING" id="273116.gene:9380991"/>
    <dbReference type="PaxDb" id="273116-14324434"/>
    <dbReference type="KEGG" id="tvo:TVG0228061"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_60_4_2"/>
    <dbReference type="PhylomeDB" id="Q97C83"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="35"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="427" mass="47011" checksum="7564FC267FEF15AA" modified="2001-10-01" version="1">MQLCVNKFKSTTGCLDGILLSVSSSHILRVARKRPVIVVSSAQFIRVLARSQIWIFVPIYLTEIRHIPVYFMGVLFFLTAVIALPFSVYGGNLIDRIGRRRVAVLIPFLVSILLFLTSISIMLRMQAIYIIIEFLSIEPLSVVQWIADSVIMADVTSEDERLDGFGILRIAGNIGFSVGPAIGGFIAQYSYAYIFLASAIGALIEFILYFAWITETGVYSRTNRKISIPFNDRAFMLVASLIGLSYFVSGQWGTTLTLFLSIVDKITNSSVGILYSINGITVVLLQIPIIRLIRKMDGFQQLAIGSIVYASGFFALAFFSSFWIIALDVVVLTIGENIVSPVSNSIVAKLAPPDKRGEYYGAMSLFVGFISPIAPVLGTSLLYRYESNSLFLWGPVFLIGIIVAIATLMIGSKLNKASNTSYITKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C87</accession>
    <name>Q97C87_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0222877 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0222877</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916472.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C87"/>
    <dbReference type="STRING" id="273116.gene:9380986"/>
    <dbReference type="PaxDb" id="273116-14324430"/>
    <dbReference type="GeneID" id="1440732"/>
    <dbReference type="KEGG" id="tvo:TVG0222877"/>
    <dbReference type="eggNOG" id="arCOG00141">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_671975_0_0_2"/>
    <dbReference type="OrthoDB" id="57215at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052528">
        <property type="entry name" value="Sugar_transport-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526">
        <property type="entry name" value="INTEGRAL MEMBRANE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="409" mass="45743" checksum="14CBC3A7596B53C8" modified="2001-10-01" version="1">MVFASSIKDVIIRLFLGSGTTEERVMDLSIGDGVLWAIYSSLTQQYVIPLAIFLFGTVAPVGFITGIPLLAVPFSQLSAYWILKKIDNLKVVTIITTFLDRLPWLLIAILILFRPAYFLYIFIALLIIRAFFASLSGTTWTLWVPGLISEEKRDRYFSVRNMAMRASGLIGYFIAMLVFIFIKFHGFDYFTIFTVSFLFSSASIAIMKKIPEAKTNDVKITSKKTYTSQKFFILLFTVSIFALGYNFSQPYILLFILGKDYLNSSSIIYTFLLIAGSLVFIASQKLGYNLISRYGYGKSLLISSIILSMTVFAWIFVRNIIEAMILFSLSSIPVSVYSLSGFNFVVSGSGGEGRVKRTSLYTLFNSISTAVGPIIANLVYVKVENILDIFLISSAILMVAAVSSWLNNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CA5</accession>
    <name>KPRS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="1 3">RPPK</shortName>
            <ecNumber evidence="1">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-phospho-D-ribosyl alpha-1-diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="1">P-Rib-PP synthase</shortName>
            <shortName evidence="1 3">PRPP synthase</shortName>
            <shortName evidence="1">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prs</name>
        <name type="ordered locus">TV0197</name>
        <name type="ORF">TVG0201915</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="Microbiol. Mol. Biol. Rev." volume="81" first="E00040" last="E00040">
            <title>Phosphoribosyl diphosphate (PRPP): biosynthesis, enzymology, utilization, and metabolic significance.</title>
            <authorList>
                <person name="Hove-Jensen B."/>
                <person name="Andersen K.R."/>
                <person name="Kilstrup M."/>
                <person name="Martinussen J."/>
                <person name="Switzer R.L."/>
                <person name="Willemoes M."/>
            </authorList>
            <dbReference type="PubMed" id="28031352"/>
            <dbReference type="DOI" id="10.1128/MMBR.00040-16"/>
        </citation>
        <scope>REVIEW</scope>
        <scope>COFACTOR</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="J. Mol. Biol." volume="413" first="844" last="856">
            <title>The structures of Thermoplasma volcanium phosphoribosyl pyrophosphate synthetase bound to ribose-5-phosphate and ATP analogs.</title>
            <authorList>
                <person name="Cherney M.M."/>
                <person name="Cherney L.T."/>
                <person name="Garen C.R."/>
                <person name="James M.N."/>
            </authorList>
            <dbReference type="PubMed" id="21963988"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2011.09.007"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.53 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOGS</scope>
        <scope>ACTIVE SITE</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916453.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="3LPN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B=1-286"/>
    </dbReference>
    <dbReference type="PDB" id="3LRT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.53 A"/>
        <property type="chains" value="A/B=1-286"/>
    </dbReference>
    <dbReference type="PDB" id="3MBI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B/C/D=1-285"/>
    </dbReference>
    <dbReference type="PDB" id="3NAG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A/B=1-286"/>
    </dbReference>
    <dbReference type="PDBsum" id="3LPN"/>
    <dbReference type="PDBsum" id="3LRT"/>
    <dbReference type="PDBsum" id="3MBI"/>
    <dbReference type="PDBsum" id="3NAG"/>
    <dbReference type="AlphaFoldDB" id="Q97CA5"/>
    <dbReference type="SMR" id="Q97CA5"/>
    <dbReference type="STRING" id="273116.gene:9380967"/>
    <dbReference type="PaxDb" id="273116-14324411"/>
    <dbReference type="GeneID" id="1441683"/>
    <dbReference type="KEGG" id="tvo:TVG0201915"/>
    <dbReference type="eggNOG" id="arCOG00067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_2_2_2"/>
    <dbReference type="OrthoDB" id="371997at2157"/>
    <dbReference type="PhylomeDB" id="Q97CA5"/>
    <dbReference type="BRENDA" id="2.7.6.1">
        <property type="organism ID" value="6326"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97CA5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <sequence length="286" mass="32177" checksum="2654A6641BAF9B66" modified="2001-10-01" version="1">MKIIALRSSLKLAARIAEELKTEPVMPDERRFPDGELYLRYDEDLTGHNIFIIGNTHSDAEVMEMILTLSAIQDYRTKSVNIIAPYYGYARQHQRYKNGEPISSQILTEIYSSYSNSIATVDIHDEKTLSYSKVKFSDLHANDAIVRYYKNVDVDYVVSPDDGGLARVADISAKLGKKHFFIEKKRIDDRTVEMKVPNVDVNGKKLLIVDDIISTGGTIAKSSGLLREKGASKIYVSAVHGLFVNGSENKILQNADEIHVTDTVESKFSDISVYQEVCNYIRDIDA</sequence>
</entry>
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    <accession>Q97CD5</accession>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
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            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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                <person name="Makino K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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        <source>
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    <sequence length="275" mass="30716" checksum="9058D1249AABE307" modified="2001-10-01" version="1">METVSAANDIYSYAISSKYQFTYYIRSKRFIGLLILTIVITAALIMLDLHYKYTTLKTENSAIFFNGFLSSDLITFFAVIAAFFGGDLVSMDLGTNTAYYTLVQPIRRSVLYIGRYTSAFLASAVMVLIVYIGGMAMSLYLYGNITDITGISFALALLYMLALLSFSSLFSAIFRTPTIGLVMSIIFLIIIYPAIQGILSGLAGVNPWMFVTYAGQILYLVFEKKYTTYAVTHVGRISFYTFNPTLLESIYIMLGYIVIFLLISAAVFTYKEIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
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            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
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        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
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                <person name="Higuchi S."/>
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                <person name="Kawamoto T."/>
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                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated Csm4 family.</text>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09663">
        <property type="entry name" value="Csm4_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005510">
        <property type="entry name" value="Csm4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01903">
        <property type="entry name" value="cas5_csm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="296" mass="33842" checksum="E93339DB2A687555" modified="2001-10-01" version="1">MSLFKISFKSSSSYVDSIKISRAIIYAYNTLYPDKIDDLINKVKDSYVSVSDLLLYKNKVYYPFPPINALIDVNNTESIKNRKKRSEFIDIDELSSIVEAFKETGYQGVNYSKIKHEGNGDNKGKPHKILISEPGINIFQTPEIKDNKYKYNDIFTKELFIYDESCFYARNSDKNIEATINLLNDFGLSGRRSTGKGEVYIEKINDNFNEGFNGEGIYILLSSFIPDNDYIKNIDFERSRYNIKTLQGKNINGNPIGPFRYFAPGSIFYLKGDVKGVSFIENNGIIPFNPVITKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CJ7</accession>
    <name>Q97CJ7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cas2</name>
        <name evidence="9" type="ORF">TVG0110650</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916361.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CJ7"/>
    <dbReference type="STRING" id="273116.gene:9380872"/>
    <dbReference type="PaxDb" id="273116-14324318"/>
    <dbReference type="GeneID" id="1441591"/>
    <dbReference type="KEGG" id="tvo:TVG0110650"/>
    <dbReference type="eggNOG" id="arCOG04194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161124_0_1_2"/>
    <dbReference type="OrthoDB" id="43236at2157"/>
    <dbReference type="PhylomeDB" id="Q97CJ7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143430">
        <property type="entry name" value="TTP0101/SSO1404-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="86" mass="10111" checksum="F2CBCFAE2B677A25" modified="2001-10-01" version="1">MYLIVVYDVDESRVNKVNKYLKRYLLWRQNSVFEGEISENLIDSMIKGITKIIKDEDSIIIYKFKVKSYIDNIIIGNDKGNTSNMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CK8</accession>
    <name>Q97CK8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">TVG0097142</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916350.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CK8"/>
    <dbReference type="STRING" id="273116.gene:9380861"/>
    <dbReference type="PaxDb" id="273116-14324307"/>
    <dbReference type="GeneID" id="1441580"/>
    <dbReference type="KEGG" id="tvo:TVG0097142"/>
    <dbReference type="eggNOG" id="arCOG02569">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015263_5_3_2"/>
    <dbReference type="PhylomeDB" id="Q97CK8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00400">
        <property type="entry name" value="Voltage_gated_ClC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000018">
        <property type="entry name" value="Chloride transporter, ClC family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050368">
        <property type="entry name" value="ClC-type_chloride_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8" id="KW-0868">Chloride</keyword>
    <keyword evidence="7" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="248"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="388"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="424"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="528"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAB59235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="592" mass="64212" checksum="83FF2C0CA8F52444" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CL6</accession>
    <name>Q97CL6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0085666 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0085666</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916342.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CL6"/>
    <dbReference type="STRING" id="273116.gene:9380852"/>
    <dbReference type="ESTHER" id="thevo-TV0085">
        <property type="family name" value="AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324299"/>
    <dbReference type="DNASU" id="1441572"/>
    <dbReference type="GeneID" id="1441572"/>
    <dbReference type="KEGG" id="tvo:TVG0085666"/>
    <dbReference type="eggNOG" id="arCOG07416">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_28_1_2"/>
    <dbReference type="OrthoDB" id="56471at2157"/>
    <dbReference type="PhylomeDB" id="Q97CL6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="201" mass="23016" checksum="BA428FD7B93DA5C1" modified="2001-10-01" version="1">MVDESYVDVSGVSVHYRFSKGSKPRKNLVIVDVNGKREWDGIRFIERIQEWGISVYYPDYPDALDSKDTLSSKSADYVKLSPKFYSDFIYSLHLKNTMLIGSGVNGDVALRCAMEYPERCSNIIVINAPGYDDYKIELYKVQKPVLLIWGDSGGYQSIMRGQSYHDLISDSVFKVFDKSAEPHVDKTEKFYSLLKNYIAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CP6</accession>
    <name>Q97CP6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">A-type ATP synthase subunit I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG0058686</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916312.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CP6"/>
    <dbReference type="STRING" id="273116.gene:9380820"/>
    <dbReference type="PaxDb" id="273116-14324269"/>
    <dbReference type="GeneID" id="1441542"/>
    <dbReference type="KEGG" id="tvo:TVG0058686"/>
    <dbReference type="eggNOG" id="arCOG04138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025558_2_1_2"/>
    <dbReference type="OrthoDB" id="85892at2157"/>
    <dbReference type="PhylomeDB" id="Q97CP6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004432">
        <property type="entry name" value="PRK05771.3-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="337"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="409"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="506"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="572"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="209"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB59197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="643" mass="72155" checksum="AF72AF4B6BB347B7" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CT6</accession>
    <name>METK_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine synthase</fullName>
            <shortName evidence="1">AdoMet synthase</shortName>
            <ecNumber evidence="1">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mat</name>
        <name type="ordered locus">TV0015</name>
        <name type="ORF">TVG0012665</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AdoMet synthase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916272.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CT6"/>
    <dbReference type="SMR" id="Q97CT6"/>
    <dbReference type="STRING" id="273116.gene:9380780"/>
    <dbReference type="PaxDb" id="273116-14324229"/>
    <dbReference type="GeneID" id="1441500"/>
    <dbReference type="KEGG" id="tvo:TVG0012665"/>
    <dbReference type="eggNOG" id="arCOG01678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057642_0_0_2"/>
    <dbReference type="OrthoDB" id="204488at2157"/>
    <dbReference type="PhylomeDB" id="Q97CT6"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.280">
        <property type="entry name" value="S-adenosylmethionine synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.340">
        <property type="entry name" value="S-adenosylmethionine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00136">
        <property type="entry name" value="S_AdoMet_synth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042543">
        <property type="entry name" value="AdoMet_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027790">
        <property type="entry name" value="AdoMet_synthase_2_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042544">
        <property type="entry name" value="AdoMet_synthase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002795">
        <property type="entry name" value="S-AdoMet_synthetase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003364">
        <property type="entry name" value="PRK04439.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003366">
        <property type="entry name" value="PRK04439.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697:SF1">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01941">
        <property type="entry name" value="AdoMet_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000150044" description="S-adenosylmethionine synthase">
        <location>
            <begin position="1"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="136"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00136"/>
        </source>
    </evidence>
    <sequence length="400" mass="44200" checksum="6C48B8ECCDA1D188" modified="2001-10-01" version="1">MERNISVEELNQIPTPKKEVEIVERKGIGHPDSVADGIAEAVSRSLSKYYLEHYGRILHHNTDQVEVVGGQSAPKYGGGLVLEPTYILLSGRATTKVGNDRVPYKSITIKAAKDYLRNNFSHLDVDADVMIDSRIGQGSVDLVEVYDTSKLEANDTSFGVGFAPLSETENIVLKTERYLNGSLKKKLPMVGYDIKVMGFRQKDTINLTVAAAFVDKYIKDADEYFNLKDQLKDLVLDNAVEETDKEVKVYINTADIRENSKSVGYLTVTGMSMENGDDGSVGRGNRVNGLITPYRAMSMEAAAGKNPVTHVGKLYNVLANKIANDIVQEEGNDIAEVLVRIVSQIGRPIDDPHVASVQVIYEGNVDHSKHKNNIRNLVNDRLAHVSDLTMQFVEGKITVF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CT9</accession>
    <name>PYRE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="1">OPRT</shortName>
            <shortName evidence="1">OPRTase</shortName>
            <ecNumber evidence="1">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrE</name>
        <name type="ordered locus">TV0012</name>
        <name type="ORF">TVG0009630</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916269.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CT9"/>
    <dbReference type="SMR" id="Q97CT9"/>
    <dbReference type="STRING" id="273116.gene:9380777"/>
    <dbReference type="PaxDb" id="273116-14324226"/>
    <dbReference type="GeneID" id="1441497"/>
    <dbReference type="KEGG" id="tvo:TVG0009630"/>
    <dbReference type="eggNOG" id="arCOG00029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_2_0_2"/>
    <dbReference type="OrthoDB" id="9089at2157"/>
    <dbReference type="PhylomeDB" id="Q97CT9"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF9">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000110793" description="Orotate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="108"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <sequence length="166" mass="18133" checksum="513265E4CC9D322F" modified="2001-10-01" version="1">MLSEDLVKSGAIKFGDFILTSGKRSDYYVDIKEAATDPNLLSEIASEFSKMIHARKIAGMELGAVPLIVATSLNMGIPYVIVRKERSHGTLSLIVGRLNRGEEVDVIEDVVTTGNSVLKAINVLRENGVVVKDAYCVVDREEGGAKLLENNGIKLHPIVRISELKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CU7</accession>
    <name>Q97CU7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cytochrome-c3 hydrogenase [sulfhydrogenase] gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0005131</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916261.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CU7"/>
    <dbReference type="STRING" id="273116.gene:9380769"/>
    <dbReference type="PaxDb" id="273116-14324218"/>
    <dbReference type="DNASU" id="1441489"/>
    <dbReference type="GeneID" id="1441489"/>
    <dbReference type="KEGG" id="tvo:TVG0005131"/>
    <dbReference type="eggNOG" id="arCOG02199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_1_1_2"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="PhylomeDB" id="Q97CU7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06221">
        <property type="entry name" value="sulfite_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050353">
        <property type="entry name" value="PyrK_electron_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513:SF1">
        <property type="entry name" value="ANAEROBIC SULFITE REDUCTASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00410">
        <property type="entry name" value="PHEHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase FAD/NAD(P)-binding" evidence="2">
        <location>
            <begin position="88"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroorotate dehydrogenase electron transfer subunit iron-sulphur cluster binding" evidence="3">
        <location>
            <begin position="204"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="244" mass="26996" checksum="BF613C206C94838D" modified="2001-10-01" version="1">METADTYTIVLTERNMGYAGQFYMVSKEGIGEAPISVGSGYGNPLMFSIKAVGSVTKGIMNDPYGEIGIRGPYGNVWPWKSYEHVVAIAGGIGIPPIKALIEDMQGHTSLDKLTVLYGARSPSDIVYRSEIETWKEEMDFRITVDKGDENWRGHVGVVTTLVPEIKEYNHGAAFVIGPPVMMKFSVKSLLDAGFAEDNIYLSLERRMECGIGVCGHCNIGRWYACEDGPIFKYSDTKEEPELFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>P58302</accession>
    <name>RAD50_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA double-strand break repair Rad50 ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rad50</name>
        <name type="ordered locus">TV0228</name>
        <name type="ORF">TVG0235331</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916483.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58302"/>
    <dbReference type="SMR" id="P58302"/>
    <dbReference type="STRING" id="273116.gene:9380999"/>
    <dbReference type="PaxDb" id="273116-14324442"/>
    <dbReference type="GeneID" id="1440743"/>
    <dbReference type="KEGG" id="tvo:TVG0235331"/>
    <dbReference type="eggNOG" id="arCOG00368">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004785_0_2_2"/>
    <dbReference type="OrthoDB" id="25344at2157"/>
    <dbReference type="PhylomeDB" id="P58302"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.510">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00449">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022982">
        <property type="entry name" value="Rad50_ATPase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002244">
        <property type="entry name" value="PRK01156.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04423">
        <property type="entry name" value="Rad50_zn_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75712">
        <property type="entry name" value="Rad50 coiled-coil Zn hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000138668" description="DNA double-strand break repair Rad50 ATPase">
        <location>
            <begin position="1"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc-hook" evidence="1">
        <location>
            <begin position="411"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="464"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="618"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="32"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00449"/>
        </source>
    </evidence>
    <sequence length="895" mass="103224" checksum="4A47DA9287E82D3A" modified="2001-09-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q977Z1</accession>
    <name>Q977Z1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1584239</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q977Z1"/>
    <dbReference type="STRING" id="273116.gene:9382341"/>
    <dbReference type="PaxDb" id="273116-14325765"/>
    <dbReference type="KEGG" id="tvo:TVG1584239"/>
    <dbReference type="eggNOG" id="arCOG03678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891297_0_0_2"/>
    <dbReference type="OrthoDB" id="52955at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046057">
        <property type="entry name" value="DUF6015"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19479">
        <property type="entry name" value="DUF6015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="150" mass="17808" checksum="E320413BF87F61F4" modified="2001-10-01" version="1">MQIIEEVSMNSKEAKPEKSGIRGYTTLEELSKAISTGLSLRDRRMSEEEAMEAAEHIINFFGYNDRVIDNMLEPEDRDSFYTMEDIGVLQTEREETTLFDGREWRIHYWILNIPRIIELANMRINTTKNKSNEEYKIYEEIPDDYWKVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q978B4</accession>
    <name>Q978B4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1557707</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978B4"/>
    <dbReference type="STRING" id="273116.gene:9382317"/>
    <dbReference type="PaxDb" id="273116-14325742"/>
    <dbReference type="DNASU" id="1442193"/>
    <dbReference type="KEGG" id="tvo:TVG1557707"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_741073_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978B4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="72"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="373" mass="42689" checksum="EBF9E0FE4100E69C" modified="2001-10-01" version="1">MRVQSNSHKGDSLSMKDPISFVEEKVKERAKETSNIKSAIIFDMDFKPPRILIRSELNKVTNDLADYVNLNLPRHIIIYGSKGSGKTLSALTIASAFKESKSIPFFYVNARENPTSIKIYRKLTRIDSRGHDIDEVKSKFDSMLSGKSIVILDEVDFLQDYDILYHLTRHTRANLILLTQKVYWYKDMNDESVKSSLQPDHIVFHEYSSEEIREILKMRADEGLNKYDKESLGLLSAMLVRDYRSDARIGIKALEIIGRLNEWDDDSVRAALKQAYVEVEGETLKNLGDRDLLILAALIKNQDTNRAYSEVSASGNMLLRGVSKSTFFQSVNYLQNLGLITLIKKKIGRYYTMESQILLSDESIVLGELRKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q978F8</accession>
    <name>Q978F8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1507408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917688.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978F8"/>
    <dbReference type="STRING" id="273116.gene:9382269"/>
    <dbReference type="PaxDb" id="273116-14325696"/>
    <dbReference type="GeneID" id="1442146"/>
    <dbReference type="KEGG" id="tvo:TVG1507408"/>
    <dbReference type="eggNOG" id="arCOG03685">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114057_1_1_2"/>
    <dbReference type="OrthoDB" id="84927at2157"/>
    <dbReference type="PhylomeDB" id="Q978F8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34352">
        <property type="entry name" value="PROTEIN YHFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34352:SF1">
        <property type="entry name" value="PROTEIN YHFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="OsmC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="153" mass="16964" checksum="D33BB737A3012BE7" modified="2001-10-01" version="1">MQVSFVYKDGEGFDSDDGKETVKITYSQGGDPNRHSPTELLLLAIGGCTSDDVLSILHKMRQDVKSYRCVVEGEKRSEHPRILKFANVSYIINGDVDPDKARRAIHLSLKKYCSVSIMAERGGVSVSYSLVLNGKPIDDRVSVEDIGELEETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q978H2</accession>
    <name>Q978H2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1492173 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1492173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054039.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978H2"/>
    <dbReference type="PaxDb" id="273116-14325682"/>
    <dbReference type="GeneID" id="24779979"/>
    <dbReference type="KEGG" id="tvo:TVG1492173"/>
    <dbReference type="HOGENOM" id="CLU_2534865_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="83" mass="8730" checksum="842D72B3E9619843" modified="2001-10-01" version="1">MNNSKIRVIAATPVHHGLHGDLTHDLISGISTSCLAGVLSTSGIRGNKQGLSLAIHTKTAFSPILARTHNKRMSASMQENLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q978I4</accession>
    <name>Q978I4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TIGR00266 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1477736</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978I4"/>
    <dbReference type="PaxDb" id="273116-14325670"/>
    <dbReference type="KEGG" id="tvo:TVG1477736"/>
    <dbReference type="eggNOG" id="arCOG01907">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040551_0_2_2"/>
    <dbReference type="PhylomeDB" id="Q978I4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00266">
        <property type="entry name" value="TIGR00266 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43657:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43657">
        <property type="entry name" value="TRYPTOPHAN RNA-BINDING ATTENUATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="TRAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="23984" checksum="401F11FFD22E245A" modified="2001-10-01" version="1">MPSMVDYEIAGQEVQYLKAKLSPEEYVYIEPGHLIYKDASVRLGASAGGLRGAFSHLLSGSAVFLLKVEGPGEIVSAGFLPGKVVRIDLNGNSIIAEFNAFLAMDSTVSYSTKFAGITQAIFGGEGVFLERFSGNGSVFLHGHGLPIVKDLKPGEEIQAEMSHLLAFEDGMEYTIERIGGLKTMLLAGAEGEGLFFARIRGPGRVWLHTISLFQLAAKLMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q978J1</accession>
    <name>Q978J1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1470916 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1470916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917656.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978J1"/>
    <dbReference type="PaxDb" id="273116-14325663"/>
    <dbReference type="DNASU" id="1442114"/>
    <dbReference type="GeneID" id="1442114"/>
    <dbReference type="KEGG" id="tvo:TVG1470916"/>
    <dbReference type="eggNOG" id="arCOG07349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387643_0_0_2"/>
    <dbReference type="OrthoDB" id="43895at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="196" mass="22311" checksum="8C4D157AB39665F5" modified="2001-10-01" version="1">MIYEIGGHEVSIVRATPGLAKSYYDLLMELYEDPNSYILVNYSKDPPQLESIVERSKNWNVHPHYMHFALYKDKVVGFAGSLLGPDYGPEVQPHIAEIYYGVSKGWRKTGLIYALLYTALSDINAKIVKATAYVENLPSINVLLKIGLRIIAVLEENDFHYMSGKMHDDYLFRGFRSDCLNRLPGLLEKHGISVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q978L2</accession>
    <name>Q978L2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1448112</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917635.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978L2"/>
    <dbReference type="SMR" id="Q978L2"/>
    <dbReference type="STRING" id="273116.gene:9382212"/>
    <dbReference type="PaxDb" id="273116-14325642"/>
    <dbReference type="DNASU" id="1442093"/>
    <dbReference type="GeneID" id="1442093"/>
    <dbReference type="KEGG" id="tvo:TVG1448112"/>
    <dbReference type="eggNOG" id="arCOG00656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040406_0_0_2"/>
    <dbReference type="OrthoDB" id="8186at2157"/>
    <dbReference type="PhylomeDB" id="Q978L2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00423">
        <property type="entry name" value="CofH family radical SAM protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="FO SYNTHASE (COFH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076:SF14">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004762">
        <property type="entry name" value="CHP00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01064">
        <property type="entry name" value="F420__menaquinone_cofactor_bio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01389">
        <property type="entry name" value="menaquinone_synthsis_involved"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="50"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="365" mass="41106" checksum="6C79E7336B23997B" modified="2001-10-01" version="1">MLSYDVDYWYERAIESPLEYSDSIDLLNMDLYDLMKLADRLTHYDAGETVSYAISYNINYTNYCTASCPICAFYVPYKNKRFGKGYELTKEQVRKEALIAKQKGATEIHIVGGFDPDLSIEYYEDIFRTIRGIMPNVAIKAFTIPEIDFIARTTGNSIKETVLRFMDAGMNAHTGGGAEIFDQEIRKYITTPEKVSGEKWLEDAKIIHSLGLKGNSTMTYGHIEGWEHIIDHMIRLRDNQIEVPGFLSFIPLKFSPENTALLRSGRVHGPAPVDKDLKVIALARIIMGRAIRNISVYWVAIGKLTAQIAINGGGNDLVGTAYSEKVFGATDRKTSTTTEELNNMIRQAGKIPGVRDTFYNIVEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q978M9</accession>
    <name>Q978M9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Muramoyltetrapeptide carboxypeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1433978</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S66 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917619.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978M9"/>
    <dbReference type="STRING" id="273116.gene:9382194"/>
    <dbReference type="MEROPS" id="S66.001"/>
    <dbReference type="PaxDb" id="273116-14325625"/>
    <dbReference type="GeneID" id="1442077"/>
    <dbReference type="KEGG" id="tvo:TVG1433978"/>
    <dbReference type="eggNOG" id="arCOG04676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034346_3_1_2"/>
    <dbReference type="OrthoDB" id="55610at2157"/>
    <dbReference type="PhylomeDB" id="Q978M9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07025">
        <property type="entry name" value="Peptidase_S66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10740">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.60">
        <property type="entry name" value="LD-carboxypeptidase A C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027461">
        <property type="entry name" value="Carboxypeptidase_A_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027478">
        <property type="entry name" value="LdcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040449">
        <property type="entry name" value="Peptidase_S66_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040921">
        <property type="entry name" value="Peptidase_S66C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003507">
        <property type="entry name" value="S66_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30237">
        <property type="entry name" value="MURAMOYLTETRAPEPTIDE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30237:SF2">
        <property type="entry name" value="MUREIN TETRAPEPTIDE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02016">
        <property type="entry name" value="Peptidase_S66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17676">
        <property type="entry name" value="Peptidase_S66C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028757">
        <property type="entry name" value="LD-carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141986">
        <property type="entry name" value="LD-carboxypeptidase A C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="LD-carboxypeptidase N-terminal" evidence="6">
        <location>
            <begin position="16"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="LD-carboxypeptidase C-terminal" evidence="7">
        <location>
            <begin position="181"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="34708" checksum="D4C8C380BAA9D863" modified="2001-10-01" version="1">MTERIKPPRLKEGDEIRVIAPASAPDMKNLSRSIARLRKAGYKVTLGHNIKRLVQMNQLSAPDVNRRDELMSAFMDDNVKAIFCARGGYGSIHILPLIDYDVIRDHPKIFVGYSDITALHLAINYKSSLITFHGPMPAADPDEFSKTNFKDFIEILKGETTHINSNSERIVRYIIQGKVDGISEGTNLSVFASLIGTGYLPNSEGKILFAEDTGVTAGDIDRYLFTMKLAGILDKFSGFAFGEFKSYVDPEDPMPFVEDIVELYMNSLKRVSLYGLPFGHGEDQMLIPLNAKVRISHEEPYVELLEEVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q978T2</accession>
    <name>Q978T2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">3-hydroxyacyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">TVG1375310</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917568.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978T2"/>
    <dbReference type="STRING" id="273116.gene:9382141"/>
    <dbReference type="PaxDb" id="273116-14325572"/>
    <dbReference type="GeneID" id="1441450"/>
    <dbReference type="KEGG" id="tvo:TVG1375310"/>
    <dbReference type="eggNOG" id="arCOG00249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010448_2_1_2"/>
    <dbReference type="OrthoDB" id="39812at2157"/>
    <dbReference type="PhylomeDB" id="Q978T2"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309:SF49">
        <property type="entry name" value="PEROXISOMAL BIFUNCTIONAL ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="13">
        <location>
            <begin position="5"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="186"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="303"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB60475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="659" mass="72977" checksum="CBBD8C9A5836EBA9" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q978W1</accession>
    <name>Q978W1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Electron transfer flavoprotein alpha-subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1345079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917539.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978W1"/>
    <dbReference type="STRING" id="273116.gene:9382111"/>
    <dbReference type="PaxDb" id="273116-14325543"/>
    <dbReference type="DNASU" id="1441421"/>
    <dbReference type="GeneID" id="1441421"/>
    <dbReference type="KEGG" id="tvo:TVG1345079"/>
    <dbReference type="eggNOG" id="arCOG00447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_1_1_2"/>
    <dbReference type="OrthoDB" id="307696at2157"/>
    <dbReference type="PhylomeDB" id="Q978W1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="349" mass="38176" checksum="16872A6573371B09" modified="2001-10-01" version="1">MTGLINAIPATNKDEYKNVGVYIEHRGDELKRPSIEMIGIGKQLARKVNEKLICVVIGDKVDGIAKEVGEYGCDIVLAAESPELAEYRTMPYAEIIGGYIQEYKPNIFLLAATRNGRDLASRIAVKSRTGITADCTVLDIDENRTLLANRPTYGESTLAEILCRNHRPQMATARPGIFPPAEKEKDHKYEVIKKKVDVDKKLLGKQILKFIPRKSTDLTSAKIVVAGGLGLGGPDGFKMLEELANLIGGAVGSSRPPVDLGWITRDHQVGQTGQSVRPDLYIAVGISGKPQHIAGMKFSKVIVSINKDPNAEITKYSDYIITEDYRKVIPALIDAIKNSKKAQAPVQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q978W3</accession>
    <name>Q978W3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ferredoxin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG1343229</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be a 3Fe-4S cluster-containing protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">To ferredoxins from P.putida and C.tartarivorum, ferredoxin I from A.vinelandii, ferredoxin II from D.desulfuricans.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917537.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978W3"/>
    <dbReference type="STRING" id="273116.gene:9382109"/>
    <dbReference type="PaxDb" id="273116-14325541"/>
    <dbReference type="GeneID" id="1441419"/>
    <dbReference type="KEGG" id="tvo:TVG1343229"/>
    <dbReference type="eggNOG" id="arCOG01984">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163428_0_0_2"/>
    <dbReference type="OrthoDB" id="7950at2157"/>
    <dbReference type="PhylomeDB" id="Q978W3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007859">
        <property type="entry name" value="ETF-QO/FixX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012206">
        <property type="entry name" value="Fd_FixX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43082">
        <property type="entry name" value="FERREDOXIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43082:SF3">
        <property type="entry name" value="FERREDOXIN-LIKE PROTEIN YDIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05187">
        <property type="entry name" value="Fer4_ETF_QO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036548">
        <property type="entry name" value="Fdx_FixX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB60444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="90" mass="10066" checksum="122FE3C6872B2F10" modified="2001-10-01" version="1">MKIEEKLSLLNYKTDKSYAHITINPDICSTCPDHFCVFACPANCYTLINGKLNFKYEDCVECGTCDIACSHGSVKWTLPKGDNGVIYKYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q978X7</accession>
    <name>Q978X7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1327879 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1327879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917522.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978X7"/>
    <dbReference type="STRING" id="273116.gene:9382094"/>
    <dbReference type="PaxDb" id="273116-14325526"/>
    <dbReference type="GeneID" id="1441404"/>
    <dbReference type="KEGG" id="tvo:TVG1327879"/>
    <dbReference type="eggNOG" id="arCOG03692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243094_0_0_2"/>
    <dbReference type="OrthoDB" id="56755at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024533">
        <property type="entry name" value="DUF3834"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12916">
        <property type="entry name" value="DUF3834"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="221" mass="24568" checksum="9825A18342218A2E" modified="2001-10-01" version="1">MEKIKAIVAPGPVSYPLIAAKAKDIDMIFDKKGQGDIVLDSTVSLVKRGINFNISLIRKLSVVYPDIGKKIGVWRKGSANDVLLRALLDLNKKESEIVYAEDQREIRNMLTQNKIDSAVLSSAFAKGKAFEDLFAETKLYMPGSCAAYVKPEILDYFNSVYSEGIDRFRQDPSGTAEYVASVLPIKFDTEFIHAAILKSESGPLEVKPYEEFRAIVLNHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q979C5</accession>
    <name>Q979C5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1277807 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1277807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916502.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979C5"/>
    <dbReference type="STRING" id="273116.gene:9382041"/>
    <dbReference type="PaxDb" id="273116-14325474"/>
    <dbReference type="GeneID" id="9284562"/>
    <dbReference type="KEGG" id="tvo:TVG1277807"/>
    <dbReference type="eggNOG" id="arCOG04414">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629930_0_0_2"/>
    <dbReference type="OrthoDB" id="56874at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011470">
        <property type="entry name" value="PRK14887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="77" mass="8475" checksum="BB119304B8646CD1" modified="2001-10-01" version="1">MHSAIIYIDQVKAEVLAPDITQPIGRAKLTIAMKGDCFYIYIEAPDTVSLKASLTSIARLIHVIEEVEGVLDGRAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q979D8</accession>
    <name>Q979D8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1258953 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1258953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917457.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979D8"/>
    <dbReference type="STRING" id="273116.gene:9382028"/>
    <dbReference type="PaxDb" id="273116-14325461"/>
    <dbReference type="DNASU" id="1441339"/>
    <dbReference type="GeneID" id="1441339"/>
    <dbReference type="KEGG" id="tvo:TVG1258953"/>
    <dbReference type="eggNOG" id="arCOG10427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121884_0_0_2"/>
    <dbReference type="OrthoDB" id="7455at2157"/>
    <dbReference type="PhylomeDB" id="Q979D8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018634">
        <property type="entry name" value="ChrB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09828">
        <property type="entry name" value="ChrB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChrB C-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="156" mass="18154" checksum="A524B96633093A16" modified="2001-10-01" version="1">MRWITREKAKVDRIACPWLISRFIDPEGEFFVPKDQVLVKGKEFGAVPFYVQGCELTYFVENGTEYVSFDPIIRKYGLDDPALLELAKIMRSADPAQPNPEKKRFGLKVLAEGFRMISKNYYENMKLQFPAYDALYAYCRSKINNSGKTRMLIQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q979D9</accession>
    <name>Q979D9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">D-3-phosphoglycerate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1257432</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979D9"/>
    <dbReference type="STRING" id="273116.gene:9382027"/>
    <dbReference type="PaxDb" id="273116-14325460"/>
    <dbReference type="KEGG" id="tvo:TVG1257432"/>
    <dbReference type="eggNOG" id="arCOG01754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_3_2"/>
    <dbReference type="PhylomeDB" id="Q979D9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12175">
        <property type="entry name" value="2-Hacid_dh_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000041">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF178">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE YGL185C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="5">
        <location>
            <begin position="32"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="6">
        <location>
            <begin position="98"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="304" mass="34150" checksum="5B3F95EFB063127C" modified="2001-10-01" version="1">MLPNFLPVGEIRKLAASVLPDINIITDLDFKSDESDILIVTAFTKVDKALLDKFPRLKFIQVASTGYDNVDVTEVKSRNILMCNSPSSNKESVAEHVIGMALYFLKDFGHLDREIRNGNWPILTFSRDLMGKVFGIIGMGAIGRKLAERLIQFGVGIIYYDVKRLPEEVEDELGITYVTLDELISSADIISIHVPLTETTKGMIGRKEFEKMKDGCIFINTSRAEVVVTKDLVWALKQGKIKAGIDVYDREPPDFSSELFQMDNALFSPHIAGVTVESQRRFIEETVANVIRYMQGVDPLNRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="146" xmlns="https://uniprot.org/uniprot">
    <accession>Q979F3</accession>
    <name>Q979F3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">TVG1237894</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="24 25" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917440.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979F3"/>
    <dbReference type="STRING" id="273116.gene:9382010"/>
    <dbReference type="PaxDb" id="273116-14325446"/>
    <dbReference type="GeneID" id="1441322"/>
    <dbReference type="KEGG" id="tvo:TVG1237894"/>
    <dbReference type="eggNOG" id="arCOG01762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_5_0_2"/>
    <dbReference type="OrthoDB" id="6009at2157"/>
    <dbReference type="PhylomeDB" id="Q979F3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019969">
        <property type="entry name" value="RNAP_Rpo2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006335">
        <property type="entry name" value="PRK08565.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03670">
        <property type="entry name" value="rpoB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 15 24" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="20">
        <location>
            <begin position="14"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="19">
        <location>
            <begin position="208"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="458"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="553"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="23">
        <location>
            <begin position="635"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="17">
        <location>
            <begin position="725"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="1098"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="894"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="894"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BAB60350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="1195" mass="134556" checksum="1B0A0EBE9296FC3E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q979F8</accession>
    <name>Q979F8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">TVG1232029</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979F8"/>
    <dbReference type="STRING" id="273116.gene:9382005"/>
    <dbReference type="PaxDb" id="273116-14325441"/>
    <dbReference type="KEGG" id="tvo:TVG1232029"/>
    <dbReference type="eggNOG" id="arCOG01180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_3_3_2"/>
    <dbReference type="PhylomeDB" id="Q979F8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAB60345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="172" mass="20369" checksum="DE35463BD2213438" modified="2001-10-01" version="1">MNIREYIEGDQRFVKQRIDRMSIISLWVRKEYTNMLTLYEKHVPVPRPIGFFKNILVEGYIGTKEKPAPQLKDVQVTEEIYTMTLDGIKKMLSARLVHSDLSEYNILYHRRRVYFIDLAQAVDIDHPMATEFLKRDIKNISTFFSKHGIKTSFEEMIQDIKNAIPKNLEQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q979G4</accession>
    <name>Q979G4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1228122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="273116.gene:9381998"/>
    <dbReference type="PaxDb" id="273116-14325435"/>
    <dbReference type="KEGG" id="tvo:TVG1228122"/>
    <dbReference type="eggNOG" id="arCOG03049">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_334841_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10098">
        <property type="entry name" value="RAPSYN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="852" mass="98511" checksum="430307C2FB9ABC2A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q979H0</accession>
    <name>Q979H0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TVG1219960 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1219960</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979H0"/>
    <dbReference type="STRING" id="273116.gene:9381992"/>
    <dbReference type="PaxDb" id="273116-14325429"/>
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    <dbReference type="eggNOG" id="arCOG05252">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105716_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
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    </dbReference>
    <dbReference type="InterPro" id="IPR019287">
        <property type="entry name" value="Hday_junct_resolvase-rel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028300">
        <property type="entry name" value="Holliday_junct_resolvase-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10107">
        <property type="entry name" value="Endonuc_Holl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014735">
        <property type="entry name" value="UCP014735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Holliday junction resolvase-related" evidence="3">
        <location>
            <begin position="28"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="190" mass="21676" checksum="4CE97E56FA323F44" modified="2001-10-01" version="1">MHHMRLIQLENIVFMNLITFLILVLAALFLVIVLLIFYIVSLKRSVLLKQHEVEMTLKKANELINEAMASREEAMKEKEEINKKIEDARRESVSKSRDVVVGRVSEQLAPFFPGFKYNPKDVRFIGTPIDLIIFDGMDNGNITSIVFLEVKSGASKLTEREKAVMEAINSGRVSFDIYRINTTNSAIDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q979I3</accession>
    <name>Q979I3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1207583 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1207583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979I3"/>
    <dbReference type="STRING" id="273116.gene:9381979"/>
    <dbReference type="PaxDb" id="273116-14325416"/>
    <dbReference type="KEGG" id="tvo:TVG1207583"/>
    <dbReference type="eggNOG" id="arCOG06957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_775233_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="342" mass="36812" checksum="132425B1EB544C0C" modified="2001-10-01" version="1">MNFNSVLRSIYYDSVISKDRNDYNLVFIFYSMKYSILLIVLVIALFMLPMVSSAASGTSEITLSTTSISIPRGGSSTVTYNVKLASGNTWGTSISASAPSGISVTFSNPTGDPPFSGTATVTVAKTVSPGTYTIEISATGDDPSSSPATLSVTVMNTTVTTAPPPAPVVHVNYIPFIVGGIAIALFVVFLIIFSLFLPAYSKPIRYVTYTVTLILSMYLITYDHVLLKYAYSHWLGLIVYLLLSTIFFGLTFSSSQALKRSSLYLLTVGNILLGLLMIVDTVFGLPYSSLHNVTTNVGWDYLFGYGTTSISTVGISLAFTLLFIFVGIYAGVSLREARVLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q979J7</accession>
    <name>Q979J7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="4">P5C reductase</shortName>
            <shortName evidence="4">P5CR</shortName>
            <ecNumber evidence="4 5">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">proC</name>
        <name evidence="9" type="ORF">TVG1190495</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
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    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="7">
        <location>
            <begin position="17"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="8">
        <location>
            <begin position="166"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="279" mass="30133" checksum="F0FD10D142056F29" modified="2001-10-01" version="1">MFIKIFLITYRYKLTVISIIGGGTIGSSIAIPLKRSGFEVIVTRRNTAVLKHLESYGISVMSDNKTASKEADVVFLTLKPVDIIPEVRKIEENIRGKLLISMAAAIPLKLIKDAAPHSIVVRAMTNIAAKVGAGFTPFCVDDGDQEVAKRASSILSALGEVYLVEEKYMDALTALSGSGPAYILTIIEAMMYGGLKLGLPRDLALKASYQTVMGSAKLLAESGEHPSELKEKVITPGGTTIDGIYELEDARVRTAIMKAIEVSTMKANKISESIKKLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q979K8</accession>
    <name>Q979K8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thioredoxin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1183005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FADPNR"/>
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        <property type="entry name" value="PNDRDTASEII"/>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="3">
        <location>
            <begin position="23"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="326" mass="34856" checksum="49FC51DE9D7F5FAC" modified="2001-10-01" version="1">MNLYRGMEFNLRSVSTEAKERDFDVIIIGAGAAGFSAAVYASRSGLSAVILDKNVAGGLTAEAPLVENYLGFKSIVGSDLAKNFAEHASEYASIREGVEVKSVKKGDGGFIVDTSDGEYHSKYIIITTGTTHKHLGVKGEAEYFGKGVSYCSTCDGYLFKNKNVVTIGGGNSGAIAAISMSEYVKNATIVEYMPRYMCENAYIEEIKKRKIPYIMNAQVTEIVGDGKKVTGVKYKDRSSGEEKTLPADGVFVYVGLIPQTSFLKDSGVKLDERGYIIVDGRQRTNVPGIYAAGDVTSGSFAQIASAVGDGCKAALSLYSDTISSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q979M4</accession>
    <name>Q979M4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH dehydrogenase I chain E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1163214</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917370.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979M4"/>
    <dbReference type="STRING" id="273116.gene:9381935"/>
    <dbReference type="PaxDb" id="273116-14325374"/>
    <dbReference type="DNASU" id="1441252"/>
    <dbReference type="GeneID" id="1441252"/>
    <dbReference type="KEGG" id="tvo:TVG1163214"/>
    <dbReference type="eggNOG" id="arCOG01546">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_1_2"/>
    <dbReference type="OrthoDB" id="15253at2157"/>
    <dbReference type="PhylomeDB" id="Q979M4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01350">
        <property type="entry name" value="NDH1_NuoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018086">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004741">
        <property type="entry name" value="PRK06076.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="NADH DEHYDROGENASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432:SF3">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00667">
        <property type="entry name" value="COMPLEX1_ND1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="346" mass="38814" checksum="C267729A0BBDC761" modified="2001-10-01" version="1">MNILLRLQLFFQITGHYASYFLAYIFETIFFLIFVFVSLIAMIYLFRKYMARLQLRYGPNRVGKFGSLQLIADAIKLVGKESILPKLRDDFAYKTAPYIVFIGLVVGFILIPYGDFYWIGSLTITHSDVSLILFFGAIAMMPIGEILAGISSHNKYALLGAMRGVAKDVSFEVPMMLSAIALVMMDSAVNKTPLEFSSLVSTQFIPFGILQPLGLFVFMVAMIARSSYTPFDLSESDSEIVSGYSTEYSGMRFGLFYMGMFGSIFLGSLVISLLYLGGYNGPYSSYAGFIYLLIKGLIMVLISFLIWLSMPRIRIDRFVNFGWKILLPIAIINLIWAGFLTLGVIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-25" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q979P0</accession>
    <name>QUEC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="1">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Archaeosine biosynthesis protein QueC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">PreQ(0) synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queC</name>
        <name type="ordered locus">TV1120</name>
        <name type="ORF">TVG1151458</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-carboxy-7-carbaguanine + NH4(+) + 2 ATP = 7-cyano-7-carbaguanine + 2 AMP + 2 diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27983"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-carboxy-7-carbaguanine + ATP + H(+) = 7-carboxyadenylyl-7-carbaguanine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:85175"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:234255"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:85176"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-carboxyadenylyl-7-carbaguanine + NH4(+) = 7-amido-7-carbaguanine + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:85179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:190446"/>
            <dbReference type="ChEBI" id="CHEBI:234255"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:85180"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-amido-7-carbaguanine + ATP = 7-iminomethyladenylyl-7-carbaguanine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:85183"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:190446"/>
            <dbReference type="ChEBI" id="CHEBI:234257"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:85184"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-iminomethyladenylyl-7-carbaguanine = 7-cyano-7-carbaguanine + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:85187"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:234257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:85188"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917354.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979P0"/>
    <dbReference type="SMR" id="Q979P0"/>
    <dbReference type="STRING" id="273116.gene:9381919"/>
    <dbReference type="PaxDb" id="273116-14325358"/>
    <dbReference type="GeneID" id="1441236"/>
    <dbReference type="KEGG" id="tvo:TVG1151458"/>
    <dbReference type="eggNOG" id="arCOG00039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081854_1_0_2"/>
    <dbReference type="OrthoDB" id="6532at2157"/>
    <dbReference type="PhylomeDB" id="Q979P0"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="QueC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00364">
        <property type="entry name" value="7-cyano-7-deazaguanine synthase QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914:SF1">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000246994" description="7-cyano-7-deazaguanine synthase">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <sequence length="241" mass="27003" checksum="7F96812FE2AEA268" modified="2001-10-01" version="1">MPKKAVVLLSGGLDSSTVLAYALNKGFEVYAISFDYGQRHLKEMKSSEAISKFYNVDRKIVKVDLRSIGKSALTDNIEVPSRDISTIEEEIPVTYVPARNTIFLSIAAAYAESLGTNEVFIGANAIDYSGYPDCRPEYFNAMENALSLGTKIGLKEKFHINVPLQYLSKADIVKLGVKLNVPYELTWSCYKGEEEACGECDSCQLRLKGFMEAGFADPLKYSKYPEFYSKNLVKLRPLKRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q979P8</accession>
    <name>GUAAA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP synthase [glutamine-hydrolyzing] subunit A</fullName>
            <ecNumber evidence="1">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaAA</name>
        <name type="ordered locus">TV1112</name>
        <name type="ORF">TVG1144148</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917346.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979P8"/>
    <dbReference type="SMR" id="Q979P8"/>
    <dbReference type="STRING" id="273116.gene:9381911"/>
    <dbReference type="MEROPS" id="C26.A31"/>
    <dbReference type="PaxDb" id="273116-14325350"/>
    <dbReference type="GeneID" id="1441228"/>
    <dbReference type="KEGG" id="tvo:TVG1144148"/>
    <dbReference type="eggNOG" id="arCOG00087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_4_2"/>
    <dbReference type="OrthoDB" id="10772at2157"/>
    <dbReference type="PhylomeDB" id="Q979P8"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000047">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing] subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01510">
        <property type="entry name" value="GMP_synthase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023686">
        <property type="entry name" value="GMP_synthase_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001975">
        <property type="entry name" value="PRK00758.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140236" description="GMP synthase [glutamine-hydrolyzing] subunit A">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01510"/>
        </source>
    </evidence>
    <sequence length="202" mass="22881" checksum="83F5B81190425182" modified="2001-10-01" version="1">MIMKIYVVDNGGQWTHREWRVLRELGVETEIVPNDIESRKLDGLDGLVLSGGAPNIDEEMDKLGSIGEYIEDHDYPVFGICVGAQFIALHYGAQVVKAKHPEFGKTLVKISEPANVLAGLPENIIAWENHNDEIKNLTSDFVLTASSETCEVQAFYHKHKPLYAVQFHPEVEHTQFGREIFKNFIAICQQHKEKLINNVNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q979P9</accession>
    <name>Q979P9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRC-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1143890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917345.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979P9"/>
    <dbReference type="STRING" id="273116.gene:9381910"/>
    <dbReference type="PaxDb" id="273116-14325349"/>
    <dbReference type="GeneID" id="1441227"/>
    <dbReference type="KEGG" id="tvo:TVG1143890"/>
    <dbReference type="eggNOG" id="arCOG02155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170070_0_0_2"/>
    <dbReference type="OrthoDB" id="68960at2157"/>
    <dbReference type="PhylomeDB" id="Q979P9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137">
        <property type="entry name" value="PRC-BARREL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137:SF1">
        <property type="entry name" value="PRC-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="84" mass="9512" checksum="43D1E4C9AC6C5165" modified="2001-10-01" version="1">MSSFEAYTIENLRGKYVYTNKGIFVGQVSDVILDFDNNVVHGLFIAKTNEKLVKDGDPISIPYNYVRSIGDIIILKSFPDLMHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Q1</accession>
    <name>Q979Q1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1142262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917343.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Q1"/>
    <dbReference type="STRING" id="273116.gene:9381908"/>
    <dbReference type="PaxDb" id="273116-14325347"/>
    <dbReference type="DNASU" id="1441225"/>
    <dbReference type="GeneID" id="1441225"/>
    <dbReference type="KEGG" id="tvo:TVG1142262"/>
    <dbReference type="eggNOG" id="arCOG00352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011784_0_0_2"/>
    <dbReference type="OrthoDB" id="147673at2157"/>
    <dbReference type="PhylomeDB" id="Q979Q1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NOG1 N-terminal helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="168"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="325" mass="37477" checksum="859E55C8A4F3A197" modified="2001-10-01" version="1">MTMQFKIPTVLRSQEIIDKSFSRASKIEEPYFPDKVERIKKEVQDRISAIESISCSHLDKLVKRFPSIERMHPFYRSLIDLMFDVDQYKIALSKVDATSANIKRISTEYIRKLRPVKDVGNANQIMRAYYGRFASLIEDLNETLLFLGRCRDYIRKLPEVRTDLKTYIIAGMPNVGKSSLLASLTTAKPKIASYPFTTKNVIIGYNESGSERIQFIDTPGILDRDFSEMNEIEKNAILAIRFIEGKIIFLFDYSTESLYTREQQEHLYEQIKNNINPNIIRVQTKLDISTEKVENIAISVNIESGLEPLRKIIFDGGVIDVRGRN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q979X0</accession>
    <name>HTPX_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protease HtpX homolog</fullName>
            <ecNumber evidence="1">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">htpX</name>
        <name type="ordered locus">TV1040</name>
        <name type="ORF">TVG1065076</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979X0"/>
    <dbReference type="SMR" id="Q979X0"/>
    <dbReference type="STRING" id="273116.gene:9381834"/>
    <dbReference type="PaxDb" id="273116-14325278"/>
    <dbReference type="KEGG" id="tvo:TVG1065076"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_4_1_2"/>
    <dbReference type="PhylomeDB" id="Q979X0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07338">
        <property type="entry name" value="M48B_HtpX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050083">
        <property type="entry name" value="HtpX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002322">
        <property type="entry name" value="PRK01265.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221">
        <property type="entry name" value="PROTEASE HTPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221:SF2">
        <property type="entry name" value="PROTEASE HTPX HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000138935" description="Protease HtpX homolog">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <sequence length="308" mass="34167" checksum="E4D9F206737F6B5F" modified="2001-10-01" version="1">MVPMASNNLAYIKLRLLSLLVGLGIAALASLIIYAVAYYLFGIYSIGIIFGVFVLVLMMDLLQWFIGPYIVDMVYRAKKADPNRYGNIIAIVEEVAKLNGIRPPTLYISEVSFPNAFAYESPIAGRRIAITRPLLGILNEDELRAVIGHEIGHLKHHDSAVIMAIGLIPTLIFYFAYTTLFAGDRRNGGSAIILALVLMVVSFLFNIMVLSVNRLRESYADANAALTIPNGARNLQTALAKIVRYGSSAKNTAASMLLFANYDMDREDVETLIDKWRTMRVGILSDLFSDHPHPAKRIRLLDKLQDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Z6</accession>
    <name>Q979Z6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Class I small heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1034799</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Z6"/>
    <dbReference type="STRING" id="273116.gene:9381807"/>
    <dbReference type="PaxDb" id="273116-14325252"/>
    <dbReference type="KEGG" id="tvo:TVG1034799"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117605_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q979Z6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041800">
        <property type="entry name" value="Hsp20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="86"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="4">
        <location>
            <begin position="91"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="183" mass="21194" checksum="6D843A10D4514E07" modified="2001-10-01" version="1">MVVDMAKKDEFDDWDDMFDEFFKDFGIDIKSLNNRFMRIWNRILNDPNITSSEPYVYGFTYRIGSDGKPIFQEFGNVPGITRGVRPIERGVREPITDINDDDKKVYLTFELPGVSKESIDLKISEYNITLNVDEDQRKYYKSIDFDYKLKPETAVAKFNNGLLDVTVEKATSKESGGRKVSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A12</accession>
    <name>Q97A12_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1020030 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1020030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A12"/>
    <dbReference type="PaxDb" id="273116-14325215"/>
    <dbReference type="KEGG" id="tvo:TVG1020030"/>
    <dbReference type="eggNOG" id="arCOG11000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1615396_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="164" mass="18358" checksum="D6718A8DEF9C3329" modified="2001-10-01" version="1">MSMSAVVASPVSLYSQTINSIDLKPNTLITNSTYYNISEELGGLYVPFNNGTIQSNSTIYVNYSIISAITIFDRYYMPQVQINTTIDGVTLYINGSFPSNFIIVNYTPGSNYAYEYSPGKPIHIVSGSSFEFVVTNMKYSKTTLSFNYIFDGIDISYYYTVVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A16</accession>
    <name>Q97A16_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sulphur transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1016719</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A16"/>
    <dbReference type="STRING" id="273116.gene:9381786"/>
    <dbReference type="PaxDb" id="273116-14325211"/>
    <dbReference type="KEGG" id="tvo:TVG1016719"/>
    <dbReference type="eggNOG" id="arCOG05632">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041737_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97A16"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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</entry>
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        </source>
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</entry>
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                <person name="Suzuki M."/>
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    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="MATE_Wzx_like"/>
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        <property type="entry name" value="O-ANTIGEN TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PST FAMILY PREDICTED COLANIC ACID TRANSPORTER"/>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="506" mass="55255" checksum="27AA5DE55BED6543" modified="2001-10-01" version="1">MPQTSSLLGIDALYQFTGAGAQLFSGALFYTVIVRFFSASQVGAVALFIAIIGLFNLIFSFGLGTAVQHFASYHLGRQEYATAKSIVFKILILGIIFSSIGLITMFYLAKPISITFMHTAQYTPIVKLLSIVLVGDVLFGLLNGSALGLQLFRASGLMNIIIWITYYSTALVLAFLLKSLYYVVVGWSVGIFLGVILYLYLIIKSTKNYKGRPRHLTPSLLFQFSIPVLLSSIISYGATYIDRFIVAGLMTLSALGVYNFALLVASSIGFIVAPLNNIMLPKFSEFYGEGNRENIKNRTAIATTVVAGVYVPAALGVAVLSKMIITLLAGKYYEEGSDAISIIMVFSALFVTSNVMTQLIAAVRETRVFIYSSAASLASNAILSIILIPHFGIEGASIGFSSIYAASFFVLYKYAKKTGFYQANVYDLAKVWGSSLVMVFVVLAFEVRFGTSIDLLVPYILLGAVVYLGMIKSTRLIHRNEALFLSSLFPQSMPFITKLIRLISSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AB2</accession>
    <name>Q97AB2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0917701</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AB2"/>
    <dbReference type="SMR" id="Q97AB2"/>
    <dbReference type="STRING" id="273116.gene:9381690"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325115"/>
    <dbReference type="DNASU" id="1441986"/>
    <dbReference type="GeneID" id="1441986"/>
    <dbReference type="KEGG" id="tvo:TVG0917701"/>
    <dbReference type="eggNOG" id="arCOG01403">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_2_2"/>
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    <dbReference type="Proteomes" id="UP000001017">
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    </dbReference>
    <dbReference type="GO" id="GO:0016757">
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="17"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="177"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="40701" checksum="BD0A462918C00471" modified="2001-10-01" version="1">MKLLWFAHRDIKHPKAGGAERTIYEVGKRLSKLNIGVNLVTVNPGNLLEYENVDGIIIYRIKGNIRTHLYVRKMIKKINPDVIIDDMGHAVPWFSPWFTDKRVIVFFRHLHAKSLPGQVNFFLAKIITLVEKLYPIIYKNNIFVTESDTSENDLIDLGIKKDRIVRIPPGVDLNLFHTGKKTDNVQLLYFGGLRKYKRPEYAITIYENLYKKIDNLKLIIVGDGILLNEIKDKVKGKNYNIVFTGKLEYDELAKIIRESWVNLHFSVTEGWGYSILEASASGTPSVAFKVPGVVDTIIDGYNGFLVNNINEFTDKILYIIKNEDKFILNSRKFAENFTWEKTADLWYKLLNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AB3</accession>
    <name>Q97AB3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0916684 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0916684</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054002.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AB3"/>
    <dbReference type="STRING" id="273116.gene:9381689"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325114"/>
    <dbReference type="GeneID" id="24779951"/>
    <dbReference type="KEGG" id="tvo:TVG0916684"/>
    <dbReference type="HOGENOM" id="CLU_1084254_0_0_2"/>
    <dbReference type="OrthoDB" id="375115at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="256" mass="29974" checksum="9ABD76DA11C8CFED" modified="2001-10-01" version="1">MPISIVTRAYKTSELKNLISNLHTNLEVEKEIIAVCRINDHDIKGINLIIENSNMFQARITGIKNANYDRILLLDSDQIPEKGLLHELDDKSEDMVIIPEKSINKNFTTKCLDDWRYRNEKYAMKNPNPYIPVVPRYYRRSVLLNVINKLSPDIYKIESHEDSILYYEVFKETQNIGFAKKYILNYDPPLKILMQKAFLYGKYRKILQNLEAPDEILMLINKINKNALHIKDLGIGRGYIVQILRGLMYELGRIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AB7</accession>
    <name>Q97AB7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0910396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
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                <person name="Kawamoto T."/>
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                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60035.1"/>
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        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055387_2_0_2"/>
    <dbReference type="OrthoDB" id="56830at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="Glyco_trans_2-like"/>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43685">
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    </dbReference>
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        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="10"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="351" mass="41439" checksum="DF798A26BECEC8AE" modified="2001-10-01" version="1">MENEKLPYISVIIIAYNRKEFLLDAIKSALNQTLDREYYEIIVIKNFQDDTIDNFIKENNIKGIISENKSLGGKISEALYVSKGEIVSFLEDDDLFFPNKLREVYDIFISNPNLVYYHNNFIKTNNRLFEIDYSENKKNKKIFLKLIELNNIKTINYLISKKLIAFNMSAITIRKSYFNDCLDILENIRIAHDMCFFFCLSGKSDTSKEILIYDKPLSIWFIHNSWSNTSCDLEIKNFKNKNFTIAKETLNAIVYIFNEFCIKNNLYNFYDNKQPLCDYLKLTISARKSQLYLLNEYEININDIKLFFKFVTMRKSIYLSGAFILVLFGKFISKKLSTKIFLYISFLLNTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
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    <name>Q97AC0_THEVO</name>
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        <submittedName>
            <fullName evidence="3">TVG0908765 protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="GeneID" id="25392638"/>
    <dbReference type="KEGG" id="tvo:TVG0908765"/>
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    <dbReference type="Proteomes" id="UP000001017">
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="17"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="105" mass="12095" checksum="8FBAA6998123DD27" modified="2001-10-01" version="1">MPDYIENLKVRKPSRDKKISLITRTGVDEIIRNCNNARDKAIISILYYSGCRVSEILSLKISDIVYEEYCVISHFHGKTGNRILGIIGYSTAYLRDYIKIKYDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AC7</accession>
    <name>Q97AC7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmatota</taxon>
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            <taxon>Thermoplasmatales</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="Transposase_mut"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="8476" checksum="BDF7DA22DB2506D6" modified="2001-10-01" version="1">MIVADGIKNLDEEVMEIYNRSEFQLCTIHYLRGLKSDVRKKDADDITNDGDKMFKCDNKEEAIIKFNEFKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AF7</accession>
    <name>Q97AF7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbon monoxide dehydrogenase subunit G</fullName>
        </recommendedName>
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    <gene>
        <name evidence="1" type="ORF">TVG0874790</name>
    </gene>
    <organism evidence="1 2">
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        <lineage>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917097.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AF7"/>
    <dbReference type="STRING" id="273116.gene:9381645"/>
    <dbReference type="PaxDb" id="273116-14325070"/>
    <dbReference type="GeneID" id="1441945"/>
    <dbReference type="KEGG" id="tvo:TVG0874790"/>
    <dbReference type="eggNOG" id="arCOG01927">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146456_0_0_2"/>
    <dbReference type="OrthoDB" id="25755at2157"/>
    <dbReference type="PhylomeDB" id="Q97AF7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05018">
        <property type="entry name" value="CoxG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CO_DH_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38588">
        <property type="entry name" value="BLL0334 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38588:SF1">
        <property type="entry name" value="BLL0334 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06240">
        <property type="entry name" value="COXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16652" checksum="21DE602CE7CEACE2" modified="2001-10-01" version="1">MDYSGSFYVDRDPAEVKQFISNFENVVACLPGIDSYTNNGNEFECRIRLDISSLSNSYLKHISGKMKFLFVPERDNGISVNGSGRIAGSSVSMNVKVFVEGSNEHAKVTWSAAIDYGLILKAMGSEKIKEVTDENIKRSIECLRGKISMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AJ9</accession>
    <name>Q97AJ9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inosine-5-monophosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0812781</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AJ9"/>
    <dbReference type="STRING" id="273116.gene:9381601"/>
    <dbReference type="PaxDb" id="273116-14325027"/>
    <dbReference type="KEGG" id="tvo:TVG0812781"/>
    <dbReference type="eggNOG" id="arCOG00601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064879_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AJ9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014651">
        <property type="entry name" value="UCP036983_2CBS_MJ1404"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF29">
        <property type="entry name" value="OS02G0818000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036983">
        <property type="entry name" value="UCP_2CBS_MJ1404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="58"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="125"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="200"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="355" mass="39942" checksum="00AF9939DD114DD1" modified="2001-10-01" version="1">MTPDPVTVGVDDTFSKVMSKMNETGIHQLPVMDGNRYAGMITYSDLLKKRSIQVKSKISNYAINTPTLSADDDVLEAVRLIKDTGLSALPVFDKGKLVGIISRTDIIKRIDKISDISNLRAFQIMSPDPVAVSEDDSIEEAFDSLRQLNEVEIPVVDNEERLVGIVKLNDILGIMFREKEKIKYGGYGEKERVQIACGSVMDPPISVDRYADVKTVVDEMMKNELHIMPIVDSGKLVGIIDFSDLINIIKTESKEGILIEISGLDIYDEDLYDIAFDLSERFLTKFSKLTDVSQGKLLIHVMKYKTQGATKYSVRTRISAPPIFMVQNDAGWNFAVVLSNIFDRYEERLKKLKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AL4</accession>
    <name>Q97AL4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TVG0795952 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0795952</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AL4"/>
    <dbReference type="STRING" id="273116.gene:9381586"/>
    <dbReference type="PaxDb" id="273116-14325012"/>
    <dbReference type="KEGG" id="tvo:TVG0795952"/>
    <dbReference type="eggNOG" id="arCOG02937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_743187_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AL4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="6">
        <location>
            <begin position="29"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="350" mass="38575" checksum="ECD72269DB899217" modified="2001-10-01" version="1">MKEKGLYRAPGKYRKEMRYGEIHYGSGYPITGLIKFLGYYDESLKIANFPSISLNTDVSEAYSAFMISKDNGNDTAVVEGENSPNITKKAMTAINVFKNLYDIKGSFHFYLRIKRKYAGAKGLGESAAVAAAASRSLVSALFEKEALKDSNFISIVARLASGSGSKSVAGPLSLWLTAPAVSHEGSFSLNLRKEIDDIFLCAVPIRDSVSTAEAHNTVIKSPFYQQWSRLQFDAVYSIISRGGYSAQIIENATTNTYLMHSVLISTGKLLWNQDTLRAMGIVEDMRRIGRLIGFSIDTGPSVLVMADREDLIKEFKERYNGECIDASVPNGAPDIPSSFVESAERYFAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AN5</accession>
    <name>Q97AN5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0780158</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AN5"/>
    <dbReference type="STRING" id="273116.gene:9381565"/>
    <dbReference type="PaxDb" id="273116-14324991"/>
    <dbReference type="KEGG" id="tvo:TVG0780158"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088582_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AN5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="265" mass="28374" checksum="D4D366707279D5CD" modified="2001-10-01" version="1">MHVLYMESSFSKGYKKDLSLFNLVMLNSMGMIGSGWLFAALYTSSYAGSFGSIVSWILGGVIVALVALTFMEVSSAFPLAGGSTPMGEFAFGKLGGFIAGWGAWISDIMTPPIEAVAMVTYLSFFVKGLITPSGILLPLGYLVVIIVLIVIFLINLQAVKVFGNVTSGIMIWKWAVPALAVITLISLAFHPSNFAIAGRFYQGYSGIFYALVLGGVVFSFEGFRGAINMAAESQHRDYIWKSVIIAVGGCACPLFSNTGSFHRCA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-11" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AN7</accession>
    <name>PDAD_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyruvoyl-dependent arginine decarboxylase</fullName>
            <shortName evidence="1">PvlArgDC</shortName>
            <ecNumber evidence="1">4.1.1.19</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="1">Pyruvoyl-dependent arginine decarboxylase subunit beta</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Pyruvoyl-dependent arginine decarboxylase subunit alpha</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdaD</name>
        <name type="ordered locus">TV0773</name>
        <name type="ORF">TVG0778593</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-arginine + H(+) = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="1">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PdaD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917020.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AN7"/>
    <dbReference type="SMR" id="Q97AN7"/>
    <dbReference type="STRING" id="273116.gene:9381563"/>
    <dbReference type="PaxDb" id="273116-14324989"/>
    <dbReference type="GeneID" id="1441868"/>
    <dbReference type="KEGG" id="tvo:TVG0778593"/>
    <dbReference type="eggNOG" id="arCOG04490">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114389_0_0_2"/>
    <dbReference type="OrthoDB" id="30748at2157"/>
    <dbReference type="PhylomeDB" id="Q97AN7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:L-arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.10">
        <property type="entry name" value="Pyruvoyl-Dependent Histidine Decarboxylase, subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01404">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016104">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016105">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase_sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002724">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00286">
        <property type="entry name" value="pyruvoyl-dependent arginine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438">
        <property type="entry name" value="PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438:SF1">
        <property type="entry name" value="PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01862">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005216">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01170">
        <property type="entry name" value="Pyruvoyl-dependent_arginine_de"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00055">
        <property type="entry name" value="Pyruvoyl-Dependent_Histidine/A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56271">
        <property type="entry name" value="Pyruvoyl-dependent histidine and arginine decarboxylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000023338" description="Pyruvoyl-dependent arginine decarboxylase subunit beta" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000023339" description="Pyruvoyl-dependent arginine decarboxylase subunit alpha" evidence="1">
        <location>
            <begin position="44"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic)" evidence="1">
        <location>
            <begin position="43"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser)" evidence="1">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01404"/>
        </source>
    </evidence>
    <sequence length="182" mass="19824" checksum="C6560128EFBF6DAF" modified="2001-10-01" version="1">MSGFVPSEIFFTRGVGRGETQLESFEAALRDAGIAQFNLSSISSIFPPHAKMVSKEEGLKKLSPGQILFTVLARNTSNELNRMISAAIGYAIPRDKSKWGYLSEHHSFGETEKVAGSFAEKLAAEMLSSTFGSTSQLIYDKEKEEYVLEDKILTTGNISATAVVLSHDEWTTVVAAAVLIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AP5</accession>
    <name>Q97AP5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0769271 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0769271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917012.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AP5"/>
    <dbReference type="STRING" id="273116.gene:9381555"/>
    <dbReference type="PaxDb" id="273116-14324981"/>
    <dbReference type="GeneID" id="1441860"/>
    <dbReference type="KEGG" id="tvo:TVG0769271"/>
    <dbReference type="eggNOG" id="arCOG05363">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_734916_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="366" mass="41709" checksum="1F8E23821A88FB84" modified="2001-10-01" version="1">MDRRDIPGLVFGLFSIVLSIGVLIYYLPDIIYKASIIAEMPSAYYALLSFKVNSLEIYTFTIISIIVLLISIISLRKFNADKTTSFSKIGGNKFFWYLSIYVFFDLLLSFIISSIYPSYGITSINSYPQFQQVYYYSVSVIFDTILFQAIPITVMLGIYAIVAKKPRIMLNSSGLNWNIIGAIAIISATYSSIIYILLGQNIFYTISIYVSSIIMTIIYVKFGFLRAFLGNFIISMVSYLSVLVHTYPLYSLLFTLYLFAWASVGIYYMFVENEARHTVQKSQKQKEEVKREPVQIPMLGPSDLFIRSVCPECGGVVFHVKEDMSLECDHCHHVLAPEDVGPFNVKVDIYGRLHDHFYEPTDIGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AQ2</accession>
    <name>Q97AQ2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">succinate dehydrogenase</fullName>
            <ecNumber evidence="5">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">TVG0761062</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="5"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917004.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AQ2"/>
    <dbReference type="STRING" id="273116.gene:9381547"/>
    <dbReference type="PaxDb" id="273116-14324973"/>
    <dbReference type="GeneID" id="1441852"/>
    <dbReference type="KEGG" id="tvo:TVG0761062"/>
    <dbReference type="eggNOG" id="arCOG00962">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_3_2_2"/>
    <dbReference type="OrthoDB" id="144910at2157"/>
    <dbReference type="PhylomeDB" id="Q97AQ2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000003">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="13" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="15">
        <location>
            <begin position="5"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="16">
        <location>
            <begin position="137"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAB59899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="240" mass="27281" checksum="617B049199893018" modified="2001-10-01" version="1">MSDEFEFEIKRKDGSGKTYFQKYNVPKDKVSTVLEGLLYIKENLDQTLSFRYSCRMEICGSCGMEIQGKPRMACSTIIEDLKSDKIRIEPLKHYKVIRDLVVDIDPFFDKYKEVKPYVIRDDDGKYDKELSQTPEQFHEYANYAMCIKCGLCMAACPIEGSDPSYLGPAPLAAAWRYIADNRDKGAKYRVEVVDGENGTARCHFAGECTEVCPKGVNPSFAIQKLRKTALKIELASIFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AR7</accession>
    <name>Q97AR7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Spermatogenesis-associated protein 20-like TRX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0747659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916989.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AR7"/>
    <dbReference type="STRING" id="273116.gene:9381532"/>
    <dbReference type="PaxDb" id="273116-14324958"/>
    <dbReference type="DNASU" id="1441837"/>
    <dbReference type="GeneID" id="1441837"/>
    <dbReference type="KEGG" id="tvo:TVG0747659"/>
    <dbReference type="eggNOG" id="arCOG02007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014051_4_1_2"/>
    <dbReference type="OrthoDB" id="28016at2157"/>
    <dbReference type="PhylomeDB" id="Q97AR7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006402">
        <property type="entry name" value="UCP006402_thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 20-like TRX" evidence="1">
        <location>
            <begin position="3"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="647" mass="74022" checksum="A0ABB841DAC9EFAE" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AV0</accession>
    <name>Q97AV0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0714900 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0714900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
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                <person name="Amano N."/>
                <person name="Koike H."/>
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                <person name="Higuchi S."/>
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                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Suzuki M."/>
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            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Koike H."/>
                <person name="Makino S."/>
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                <person name="Yamamoto Y."/>
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                <person name="Suzuki M."/>
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        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
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            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="561" mass="61112" checksum="5D58DF3065C0B7B2" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B02</accession>
    <name>Q97B02_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-responsive regulatory protein [Lrp]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0651564</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916916.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B02"/>
    <dbReference type="STRING" id="273116.gene:9381445"/>
    <dbReference type="PaxDb" id="273116-14324873"/>
    <dbReference type="GeneID" id="1441764"/>
    <dbReference type="KEGG" id="tvo:TVG0651564"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OrthoDB" id="6762at2157"/>
    <dbReference type="PhylomeDB" id="Q97B02"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="143" mass="16300" checksum="8CC965F5479D5F94" modified="2001-10-01" version="1">MDEKDLQILNYLMENGRDKISDMSRTLDIPRVTIYERMQNMIKSGIIKKFTIVPDYKALGIPVVAFILVAYTPKDGISQRDLAAKIATFKEVENVYIIAGEWDLLIKVRESSTEAIGNFVLDKLREMPGIERAQTITVFSEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B03</accession>
    <name>Q97B03_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0651504 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0651504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916915.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B03"/>
    <dbReference type="STRING" id="273116.gene:9381444"/>
    <dbReference type="PaxDb" id="273116-14324872"/>
    <dbReference type="GeneID" id="1441763"/>
    <dbReference type="KEGG" id="tvo:TVG0651504"/>
    <dbReference type="eggNOG" id="arCOG02201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136112_0_0_2"/>
    <dbReference type="OrthoDB" id="56913at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="244" mass="27647" checksum="15A5AB9D187BA374" modified="2001-10-01" version="1">MRLIQRRSVFREERYIDPPTVAHEYYSCRTNVVVVSFGNAGFRILRKMNPIDIRNVESYAVFRGTPARNSYYNGSLNVKGREISFEVYSIENIKNPFLKYSANPIKIMDGLTEEDALYAAQKLKSTGISFVLVSGFGGSFAQYAHSKFLEILNKSGREALSIIVVPSSKEGNRREKAISAINDLRATGNNIVVFDNDKFSFGYNFQNIMEKIEKANTMIANRLDEYISEISLSIDRLKYAIISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B20</accession>
    <name>Q97B20_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TVG0632449 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0632449</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053946.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B20"/>
    <dbReference type="STRING" id="273116.gene:9381427"/>
    <dbReference type="PaxDb" id="273116-14324855"/>
    <dbReference type="GeneID" id="1441745"/>
    <dbReference type="KEGG" id="tvo:TVG0632449"/>
    <dbReference type="eggNOG" id="arCOG04034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_447341_0_0_2"/>
    <dbReference type="OrthoDB" id="57449at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase HerA central" evidence="7">
        <location>
            <begin position="449"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="669" mass="76271" checksum="1A55161A1CA14272" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B32</accession>
    <name>Q97B32_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0620303 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0620303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916886.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B32"/>
    <dbReference type="PaxDb" id="273116-14324843"/>
    <dbReference type="DNASU" id="1441734"/>
    <dbReference type="GeneID" id="1441734"/>
    <dbReference type="KEGG" id="tvo:TVG0620303"/>
    <dbReference type="eggNOG" id="arCOG00568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_415415_0_0_2"/>
    <dbReference type="OrthoDB" id="56753at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="666" mass="75147" checksum="2643F13873F5B65D" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B41</accession>
    <name>EF1B_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Elongation factor 1-beta</fullName>
            <shortName>EF-1-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>aEF-1beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ef1b</name>
        <name type="ordered locus">TV0618</name>
        <name type="ORF">TVG0610740</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59760" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59760.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053942.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B41"/>
    <dbReference type="SMR" id="Q97B41"/>
    <dbReference type="STRING" id="273116.gene:9381406"/>
    <dbReference type="PaxDb" id="273116-14324834"/>
    <dbReference type="GeneID" id="1441724"/>
    <dbReference type="KEGG" id="tvo:TVG0610740"/>
    <dbReference type="eggNOG" id="arCOG01988">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165896_2_0_2"/>
    <dbReference type="OrthoDB" id="84643at2157"/>
    <dbReference type="PhylomeDB" id="Q97B41"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00043">
        <property type="entry name" value="EF1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004542">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00489">
        <property type="entry name" value="aEF-1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001670">
        <property type="entry name" value="PRK00435.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006521">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155071" description="Elongation factor 1-beta">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="88" mass="9766" checksum="762672C3901AF04F" modified="2002-03-27" version="2">MADVLVSFKLLPSDSDVDTSVMESEVKEKLNGVCKINNIEEKDIGFGLKYIHLEVIVEDKEGEVDRIEKVLSMVKGVGEINTENVSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B78</accession>
    <name>Q97B78_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0567488</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916838.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B78"/>
    <dbReference type="STRING" id="273116.gene:9381365"/>
    <dbReference type="PaxDb" id="273116-14324794"/>
    <dbReference type="GeneID" id="1441096"/>
    <dbReference type="KEGG" id="tvo:TVG0567488"/>
    <dbReference type="eggNOG" id="arCOG04394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127515_4_1_2"/>
    <dbReference type="OrthoDB" id="57062at2157"/>
    <dbReference type="PhylomeDB" id="Q97B78"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37691">
        <property type="entry name" value="BLR3518 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37691:SF1">
        <property type="entry name" value="BLR3518 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="107" mass="11776" checksum="1EE80F0FBD03BC98" modified="2001-10-01" version="1">MKVITNVTDPSKVGQVVRNLMNLLREDPSIEVEVVFHVDAITVLSAESENKDVYALLKNGINVVACRNSMIAKGMDSDSLILGVTVVNAGIYELVKKISEGWIYIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B79</accession>
    <name>Q97B79_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0567190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916837.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B79"/>
    <dbReference type="STRING" id="273116.gene:9381364"/>
    <dbReference type="PaxDb" id="273116-14324793"/>
    <dbReference type="DNASU" id="1441095"/>
    <dbReference type="GeneID" id="1441095"/>
    <dbReference type="KEGG" id="tvo:TVG0567190"/>
    <dbReference type="eggNOG" id="arCOG01018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056931_3_0_2"/>
    <dbReference type="OrthoDB" id="372184at2157"/>
    <dbReference type="PhylomeDB" id="Q97B79"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18093">
        <property type="entry name" value="SpoU-like_TrmJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00050">
        <property type="entry name" value="rRNA_methyl_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786:SF2">
        <property type="entry name" value="TRNA (CYTIDINE_URIDINE-2'-O-)-METHYLTRANSFERASE TRMJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="TRNA/RRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="5">
        <location>
            <begin position="11"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="250" mass="28624" checksum="6B22DC4497DB4C77" modified="2001-10-01" version="1">MQKTDGLADRIWVVLVQPKYQGNIGAVARSMKNSGLSKLIIVGNRPDDEAFARSMGGRQILENAEIVDSLEEIVHRFTIVAGTSSTASSNLKRFRRLPVDPETFWKQMESQTGKVALVFGREDDGLRNEELDLCNYFIYIPGNPDYPVYNLSHAVAIVLYVMYRSYVPSDMIQHESIKPEHEVLLMSNILDIMKLSRYPAHKMRNAEVMLRRIIGRSMITEREYFKLMGIVRRIKEAIEGNLDEGNNERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B85</accession>
    <name>Q97B85_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="8">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">TVG0559784</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 15">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RAD25/XPB subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="8"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916831.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B85"/>
    <dbReference type="STRING" id="273116.gene:9381358"/>
    <dbReference type="PaxDb" id="273116-14324787"/>
    <dbReference type="GeneID" id="1441089"/>
    <dbReference type="KEGG" id="tvo:TVG0559784"/>
    <dbReference type="eggNOG" id="arCOG00874">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008213_6_0_2"/>
    <dbReference type="OrthoDB" id="56011at2157"/>
    <dbReference type="PhylomeDB" id="Q97B85"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17926">
        <property type="entry name" value="DEXHc_RE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18789">
        <property type="entry name" value="SF2_C_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032438">
        <property type="entry name" value="ERCC3_RAD25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040699">
        <property type="entry name" value="XPB_DRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16203">
        <property type="entry name" value="ERCC3_RAD25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18458">
        <property type="entry name" value="XPB_DRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="82"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="314"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAB59714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="455" mass="52010" checksum="88A555B73DC283DA" modified="2001-10-01" version="1">MIELTYSGGSILIRGYEGELPPDIAIFDNRIMCYRALGMNYEKVAEYLKSKGKEFTDNVCNCPDIEVKANIKLRPYQSDALDKWASSNMRGTIVLPTAAGKTHIGIEAINRTKKKTIILVPTIDLLTQWKQKIKELLNLDAGQIGGGEKEEREITVSTYDSAYLMAEKYGNHFDLIIADEVHHLASEKLRMIAMMYTARYRLGLTATYERTDGLEKDLEYLMGGKIFELGYEEIDEYLADYKIVRIPVELDDDEQTQYEKYHSIFVNYLRKHRMAIGGSWDFNKFIRSSWTPEGREALTAWRKSREIAMNPAGKIEMARTLVAQHKNERIIIFSEDTETTYRISKEFLIPAITYMTSKDERSEIPELFKTGKYNVIATSRVLDEGVDFPDASVAIVMSGSGSTRQFRQRLGRILRPSYGKKAVMYELVTSQTAEFGASRRRRKGVPSRTTSSEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B88</accession>
    <name>Q97B88_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription initiation factor IIB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0557275</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916828.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B88"/>
    <dbReference type="STRING" id="273116.gene:9381355"/>
    <dbReference type="PaxDb" id="273116-14324784"/>
    <dbReference type="GeneID" id="1441086"/>
    <dbReference type="KEGG" id="tvo:TVG0557275"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1232827_0_0_2"/>
    <dbReference type="OrthoDB" id="56686at2157"/>
    <dbReference type="PhylomeDB" id="Q97B88"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="135"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="224" mass="25098" checksum="DB36E3A8E14631EF" modified="2001-10-01" version="1">MIEGQERNILVDDFLAKLRRYKGAKIPNNVSRNENLNRIALSLKRSGLIDEIPLEVMKNALNIYLDNVSHGISRGRGCTAVLAACILVASRSSPSPVTLRYVSRALKIDPSRIWRTESLVVKNTNGKNMAVRPIDFIERYAESLNVDDDVRDEAIALTNKIASQVSLMRKSPSTIAISALYLASILKDRKLVQRYVTKKTGITEVTLIRTYKEIYSYLTGNQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B92</accession>
    <name>Q97B92_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum cofactor biosynthesis protein moeA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0553325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B92"/>
    <dbReference type="STRING" id="273116.gene:9381351"/>
    <dbReference type="PaxDb" id="273116-14324780"/>
    <dbReference type="KEGG" id="tvo:TVG0553325"/>
    <dbReference type="eggNOG" id="arCOG00216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_2"/>
    <dbReference type="PhylomeDB" id="Q97B92"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF19">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN MJ0666-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="3">
        <location>
            <begin position="178"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="391" mass="42773" checksum="79BC9BA181FEE99B" modified="2001-10-01" version="1">MKKYSEALLAGSKLNLNVRMKEVRSDISAGMYSAIPILSPSDFPQYDRSAVDGFAVRYEDVLSAGLNNPISLKLVGSIHVGDANNIRIGKNQCAEIFTGAEVPEGADAVVMQEDAEIEGEYVAIFRKVKKFQNIGRKGEDLKTGDKIIDRGQRIMPWHIPAFIETGIEKVNVFDLKIGVLSTGDEIVSGKVKNASAPMLQSLIVRDGFNSVFLGNVEDNVEKISNVIRSFEGDLLIVTGGSGPSSTDLMDSILQNLGQIIFHGIRMKPGRTTGLALVNDKPIFTISGLPVAALIAYENVIRPLIFNWLSIKETRPNIVKGELTRSIFNNEAMKMFVRVRLSNKDGKIYVDPLRTTGSGIINSVINADGYLIIDENVEGYQEGQLVEVKLIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BA2</accession>
    <name>RS8E_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S8e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps8e</name>
        <name type="ordered locus">TV0555</name>
        <name type="ORF">TVG0543769</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916813.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BA2"/>
    <dbReference type="SMR" id="Q97BA2"/>
    <dbReference type="STRING" id="273116.gene:9381340"/>
    <dbReference type="PaxDb" id="273116-14324770"/>
    <dbReference type="DNASU" id="1441071"/>
    <dbReference type="GeneID" id="1441071"/>
    <dbReference type="KEGG" id="tvo:TVG0543769"/>
    <dbReference type="eggNOG" id="arCOG04154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080597_2_1_2"/>
    <dbReference type="OrthoDB" id="372305at2157"/>
    <dbReference type="PhylomeDB" id="Q97BA2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11382">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00029">
        <property type="entry name" value="Ribosomal_eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_eS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020919">
        <property type="entry name" value="Ribosomal_protein_eS8_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000122283" description="Small ribosomal subunit protein eS8">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="127" mass="14028" checksum="761BB8F3E2FCF677" modified="2001-10-01" version="1">MTIFQGKSGKKPTGGNLKQAKKKRRFELGREPTLTKLGTKVERKVIRTMGGNSKAILFTADTANVYDIHEKKIKKVKIITVKENPANSHYVQRNIINKGTIISTEIGEAIVTSRPGQDGVINAKLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BA5</accession>
    <name>Q97BA5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2070 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0541998</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BA5"/>
    <dbReference type="STRING" id="273116.gene:9381337"/>
    <dbReference type="TCDB" id="9.B.248.1.3">
        <property type="family name" value="the duf2070 (duf2070) family"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324767"/>
    <dbReference type="DNASU" id="1441068"/>
    <dbReference type="KEGG" id="tvo:TVG0541998"/>
    <dbReference type="eggNOG" id="arCOG04351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_481131_0_0_2"/>
    <dbReference type="OrthoDB" id="8914at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019204">
        <property type="entry name" value="DUF2070_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09843">
        <property type="entry name" value="DUF2070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="556"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2070" evidence="2">
        <location>
            <begin position="27"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="575" mass="65302" checksum="AD64B84B515EC3A8" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BB8</accession>
    <name>Q97BB8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agmatine ureohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0528110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2010-12" db="PDB data bank">
            <title>The crystal structure of agmatine ureohydrolase of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Zhang Z."/>
                <person name="Burley S.K."/>
                <person name="Swaminathan S."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 2-303 IN COMPLEX WITH MN(2+)</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916796.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="3PZL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B/C=2-303"/>
    </dbReference>
    <dbReference type="PDBsum" id="3PZL"/>
    <dbReference type="AlphaFoldDB" id="Q97BB8"/>
    <dbReference type="SMR" id="Q97BB8"/>
    <dbReference type="STRING" id="273116.gene:9381323"/>
    <dbReference type="PaxDb" id="273116-14324753"/>
    <dbReference type="GeneID" id="1441054"/>
    <dbReference type="KEGG" id="tvo:TVG0528110"/>
    <dbReference type="eggNOG" id="arCOG01700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_0_2_2"/>
    <dbReference type="OrthoDB" id="7186at2157"/>
    <dbReference type="PhylomeDB" id="Q97BB8"/>
    <dbReference type="EvolutionaryTrace" id="Q97BB8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11593">
        <property type="entry name" value="Agmatinase-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="8" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="3PZL"/>
        </source>
    </evidence>
    <sequence length="303" mass="33736" checksum="C95EAD1B947C504D" modified="2001-10-01" version="1">MGGNGREKDHASELRSIFSLKKIADAVNGYEEAKYVVFGIPFDNTSSYRRGSKYAPDSIRGAYVNLESYEYSYGIDLLASGMADLGDMEESEDVEYVIDTVESVVSAVMSDGKIPIMLGGEHSITVGAVRALPKDVDLVIVDAHSDFRSSYMGNKYNHACVTRRALDLLGEGRITSIGIRSVSREEFEDPDFRKVSFISSFDVKKNGIDKYIEEVDRKSRRVYISVDMDGIDPAYAPAVGTPEPFGLADTDVRRLIERLSYKAVGFDIVEFSPLYDNGNTSMLAAKLLQVFIASREKYYKEHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BD4</accession>
    <name>Q97BD4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0512809</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916780.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BD4"/>
    <dbReference type="STRING" id="273116.gene:9381306"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324737"/>
    <dbReference type="DNASU" id="1441038"/>
    <dbReference type="GeneID" id="1441038"/>
    <dbReference type="KEGG" id="tvo:TVG0512809"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_0_2"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="PhylomeDB" id="Q97BD4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="15"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="183"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="362" mass="41488" checksum="68580CC7D2C4C68B" modified="2001-10-01" version="1">MKIAYFSDTYYPTPDGVSTYLKDVKRGLEKRGHEVYIFSLTGDPHEKNVFIPRTVPFPPYDQYRMPLIPFPFREFKTAVDIMPDVIHLHNAFYMSSVGYLVARRLGKKPISTFHTDIDKMRESIRLPFSDLIFDLGKRYSIYLYRKCKFVLAPSCQVKAYLERCGLKNIVTVPLFVDTDELSPAKASNKQDIILYLGRLTKDKGVYRVLELANEMKGTGYKFIIAGVGPELERMKAYVKENDISNVNILGYVKEDEKKQLMEVSKFFIYPSSADTFGISVFEALSMGLPAIVSKEFPVKEDTNAIVYEDFSNIKHIANKLLNIDQATYSEMSTSARTLAISKYSIDRHIDQLLSLYMSLVDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BF1</accession>
    <name>Q97BF1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TIGR01777 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0493451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BF1"/>
    <dbReference type="STRING" id="273116.gene:9381288"/>
    <dbReference type="PaxDb" id="273116-14324720"/>
    <dbReference type="KEGG" id="tvo:TVG0493451"/>
    <dbReference type="eggNOG" id="arCOG03096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047373_0_2_2"/>
    <dbReference type="PhylomeDB" id="Q97BF1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013549">
        <property type="entry name" value="DUF1731"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010099">
        <property type="entry name" value="SDR39U1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01777">
        <property type="entry name" value="yfcH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11092:SF0">
        <property type="entry name" value="EPIMERASE FAMILY PROTEIN SDR39U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11092">
        <property type="entry name" value="SUGAR NUCLEOTIDE EPIMERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08338">
        <property type="entry name" value="DUF1731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="75"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1731" evidence="3">
        <location>
            <begin position="311"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="40200" checksum="88D01BD49F1933F2" modified="2001-10-01" version="1">MYLTVTPLSIVALTTAPPYFDTNGSISVPPPPNVIRSGLYESITLEYHKYTIKYCLTLITQTILFFLIVVCSMKVVIFGGTGLIGRAIYSSLPHDFYIVSRSAKGQEGQAHIIGLDEIDKIKECDVVINLSGYSIASKRWNRKVKEEIVRSRIDTTRLVVNFIRNLEKKPSAFLSGSAIGFYGHNTNKELTEEDPFGSGFLADLARSWEEEAMQADGICRTVLLRTANFLSPKGGFLSALINPKSKNVMYFGDGRQPVSWMHYADYVKAVEFVIENASIAGPVNMSSPQPVTFKEFAEAFSKSLGSHIRSIPEFLGKIALGSEMYRELMSGQKVIPKKLIQAGFKFDFPDINTAASSIASLMK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BH3</accession>
    <name>RS24_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S24e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rps2e</name>
        <name type="ordered locus">TV0482</name>
        <name type="ORF">TVG0468673</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916741.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BH3"/>
    <dbReference type="SMR" id="Q97BH3"/>
    <dbReference type="STRING" id="273116.gene:9381264"/>
    <dbReference type="PaxDb" id="273116-14324697"/>
    <dbReference type="GeneID" id="1440999"/>
    <dbReference type="KEGG" id="tvo:TVG0468673"/>
    <dbReference type="eggNOG" id="arCOG04182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107248_3_1_2"/>
    <dbReference type="OrthoDB" id="27533at2157"/>
    <dbReference type="PhylomeDB" id="Q97BH3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018098">
        <property type="entry name" value="Ribosomal_eS24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00529">
        <property type="entry name" value="RIBOSOMAL_S24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000137659" description="Small ribosomal subunit protein eS24">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="99" mass="11602" checksum="105871F82103C586" modified="2001-10-01" version="1">MVELVITEKRDNPILKRKELKYTLKFENARTPSREEIKEIIAKHEGAQKELIIVESNKQLTGKHEIVGYTKIYQDKASAMLYEPDYELIRNGLKQKEAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-05" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BK5</accession>
    <name>NDK_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name type="ordered locus">TV0450</name>
        <name type="ORF">TVG0438422</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916710.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BK5"/>
    <dbReference type="SMR" id="Q97BK5"/>
    <dbReference type="STRING" id="273116.gene:9381231"/>
    <dbReference type="PaxDb" id="273116-14324665"/>
    <dbReference type="GeneID" id="1440968"/>
    <dbReference type="KEGG" id="tvo:TVG0438422"/>
    <dbReference type="eggNOG" id="arCOG04313">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060216_6_3_2"/>
    <dbReference type="OrthoDB" id="6874at2157"/>
    <dbReference type="PhylomeDB" id="Q97BK5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000003">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000137105" description="Nucleoside diphosphate kinase">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <sequence length="147" mass="16664" checksum="A9798CA986236CFD" modified="2001-10-01" version="1">MDRTLVLLKPDAVKRRLVGKIIERFEEKGLKIVAMKFMQMTKDQAKTHYSVHQNKPFFNDLVNYITSGPIVAMILEGAHAIEIVRLMSGATDGSKAQPGTIRGDYSMGIEKNIIHASDSLEAYNHEMPIFFSDNEIIEWAYGDEVIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-10-11" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BQ3</accession>
    <name>ENDA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA-splicing endonuclease</fullName>
            <ecNumber evidence="2">4.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">tRNA-intron endonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">endA</name>
        <name type="ordered locus">TV0402</name>
        <name type="ORF">TVG0390826</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916658.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BQ3"/>
    <dbReference type="SMR" id="Q97BQ3"/>
    <dbReference type="STRING" id="273116.gene:9381180"/>
    <dbReference type="PaxDb" id="273116-14324617"/>
    <dbReference type="GeneID" id="1440916"/>
    <dbReference type="KEGG" id="tvo:TVG0390826"/>
    <dbReference type="eggNOG" id="arCOG01701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965070_0_0_2"/>
    <dbReference type="OrthoDB" id="46045at2157"/>
    <dbReference type="PhylomeDB" id="Q97BQ3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01834">
        <property type="entry name" value="EndA_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023516">
        <property type="entry name" value="tRNA_splic_arch_long"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006797">
        <property type="entry name" value="PRK09300.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000109491" description="tRNA-splicing endonuclease">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01834"/>
        </source>
    </evidence>
    <sequence length="292" mass="33923" checksum="CC93607C8E2966E1" modified="2001-10-01" version="1">MSDGLCNGISFNVSEGHGPNYIVGRYKLGFVQQGILFLDPYECLYLYFKGRITFEDSEESALKKLFSITSVERYTAYTLLKNKGYRVHEDNGTIFFRKSTEIPRSVVVVREYDEIDVGKFFTERPDFYFTVDEEGDATIYRIDEIDIKGTENNKIGKCSVTDFGDRHFTKDDLPHWMGTKFHEYRMLTDFEANLIEGKEPKGMAEEVYRDLLSRGCIVKTGFKYGTNFRVYEGEKSEHAEYLVSVVENKLIWYYISRAVRVASSVRKKMIFTTIIDGKIKYIMISRSKDIVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-15" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BQ4</accession>
    <name>RS2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>30S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rps2</name>
        <name type="ordered locus">TV0401</name>
        <name type="ORF">TVG0390580</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59543" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59543.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916657.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BQ4"/>
    <dbReference type="SMR" id="Q97BQ4"/>
    <dbReference type="STRING" id="273116.gene:9381179"/>
    <dbReference type="PaxDb" id="273116-14324616"/>
    <dbReference type="GeneID" id="1440915"/>
    <dbReference type="KEGG" id="tvo:TVG0390580"/>
    <dbReference type="eggNOG" id="arCOG04245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058171_3_0_2"/>
    <dbReference type="OrthoDB" id="371797at2157"/>
    <dbReference type="PhylomeDB" id="Q97BQ4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000030">
        <property type="entry name" value="30S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_A">
        <property type="entry name" value="Ribosomal_uS2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023454">
        <property type="entry name" value="Ribosomal_uS2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000134337" description="Small ribosomal subunit protein uS2">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="199" mass="22450" checksum="4D19ECF715C3C16B" modified="2002-05-15" version="2">MEEEMLIPEEEYQKSGVHIGTQVKSSDMKPYIFKIRNDGLYILDVKKTNSKLIAAGKMLARFDPQDILVVAQRQYAFRPVAKFAEVTGAVSITGRFNPGTLTNPSLKFYKEVKVIVVTDPLADVQAMKEAIKIGIPIIALCDANNKTDFVDLVIPTNNKGRRSLAVIYWLLAREILKNRGTITSYDQFKYTIDDFEAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BR5</accession>
    <name>Q97BR5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transport system ATP-binding protein PA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0376753</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916644.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BR5"/>
    <dbReference type="STRING" id="273116.gene:9381167"/>
    <dbReference type="PaxDb" id="273116-14324605"/>
    <dbReference type="GeneID" id="1440902"/>
    <dbReference type="KEGG" id="tvo:TVG0376753"/>
    <dbReference type="eggNOG" id="arCOG00193">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OrthoDB" id="10909at2157"/>
    <dbReference type="PhylomeDB" id="Q97BR5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03293">
        <property type="entry name" value="ABC_NrtD_SsuB_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050166">
        <property type="entry name" value="ABC_transporter_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42788:SF13">
        <property type="entry name" value="ALIPHATIC SULFONATES IMPORT ATP-BINDING PROTEIN SSUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42788">
        <property type="entry name" value="TAURINE IMPORT ATP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="21"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="270" mass="29975" checksum="448017C71503C12E" modified="2001-10-01" version="1">MAKDLVRESQIEKPSNSSSVLEAANIDFSYENGYKVFEDINLSLNRNEFVAIVGPSGIGKSTLLRILGGFLKPDKGSVYLMGKKINRPTPDIVLIHQSIVTFPWMTALENVMVSLKAKHIPNDKAEEMAKEALETVGLQGFEDLYPKEMSGGMRQRVAIARALAADPLVFLMDEPFAHLDELTAEGLRQDIYNILFSPDTPLKGVVLVSHNLTEVIELADVIYILNNVPATVVGKVQVDLPRPRSPRSEAFNRLLDSLYNYLTPPKVKKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BV2</accession>
    <name>KADA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Adenylate kinase</fullName>
            <shortName>AK</shortName>
            <ecNumber>2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">adkA</name>
        <name type="ordered locus">TV0353</name>
        <name type="ORF">TVG0345885</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916607.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BV2"/>
    <dbReference type="SMR" id="Q97BV2"/>
    <dbReference type="STRING" id="273116.gene:9381130"/>
    <dbReference type="PaxDb" id="273116-14324568"/>
    <dbReference type="GeneID" id="1440865"/>
    <dbReference type="KEGG" id="tvo:TVG0345885"/>
    <dbReference type="eggNOG" id="arCOG01039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119371_0_0_2"/>
    <dbReference type="OrthoDB" id="26198at2157"/>
    <dbReference type="PhylomeDB" id="Q97BV2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00234">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023477">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003122">
        <property type="entry name" value="PRK04040.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13207">
        <property type="entry name" value="AAA_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000131829" description="Adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="181" mass="20210" checksum="E59FB1484B0C2DA4" modified="2001-10-01" version="1">MRSVITGVAGVGKTTVLDIVARESGIPVVNYGTLMFEVAKRRGLVENRDQIRKLSRETQVDLQKLAGEEIGKMENAIVDTHMSIKTPFGYLPGLPEWVLRSINASVFVIIEADPAIIKRRRDNDPTRARDDEGVDSIREHQEMNRYFAAAYSIFSGATVKIVKNEEGKPEQAADEIVRVIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BV4</accession>
    <name>RL15_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl15</name>
        <name type="ordered locus">TV0351</name>
        <name type="ORF">TVG0343601</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916605.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BV4"/>
    <dbReference type="SMR" id="Q97BV4"/>
    <dbReference type="STRING" id="273116.gene:9381128"/>
    <dbReference type="PaxDb" id="273116-14324566"/>
    <dbReference type="GeneID" id="1440863"/>
    <dbReference type="KEGG" id="tvo:TVG0343601"/>
    <dbReference type="eggNOG" id="arCOG00779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109163_0_0_2"/>
    <dbReference type="OrthoDB" id="9418at2157"/>
    <dbReference type="PhylomeDB" id="Q97BV4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_uL15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000104877" description="Large ribosomal subunit protein uL15">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="145" mass="15767" checksum="0CDA5AE0EA541AA7" modified="2001-10-01" version="1">MVRERTKKLRGGHYGRGMKAGRGKGKKGGRGNAGMGKHKWIWMVKYDPLHFGGKGFTSHHLSTPDVPINLGELENIFENLKADGFVREENGETVVDLKAAGYDKLLGSGNFSVKSRIIIDKATEKAISKLSAIGSKIENVGNTAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-15" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BZ1</accession>
    <name>RL21_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL21</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L21e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl21e</name>
        <name type="ordered locus">TV0314</name>
        <name type="ORF">TVG0322585</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916570.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BZ1"/>
    <dbReference type="SMR" id="Q97BZ1"/>
    <dbReference type="STRING" id="273116.gene:9381089"/>
    <dbReference type="PaxDb" id="273116-14324529"/>
    <dbReference type="GeneID" id="1440828"/>
    <dbReference type="KEGG" id="tvo:TVG0322585"/>
    <dbReference type="eggNOG" id="arCOG04129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103610_1_1_2"/>
    <dbReference type="OrthoDB" id="6295at2157"/>
    <dbReference type="PhylomeDB" id="Q97BZ1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00369">
        <property type="entry name" value="Ribosomal_eL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022856">
        <property type="entry name" value="Ribosomal_eL21_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_eL21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003303">
        <property type="entry name" value="PRK04306.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000149705" description="Large ribosomal subunit protein eL21">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="98" mass="10915" checksum="825AFED1E3DE2C9C" modified="2001-10-01" version="1">MVKMSHGPRSGSRRKLTKSKEERKKSIIGRMMQEFSPGDRVAIDIEPSVHNGMPYHRFQGYTGIVEGAQGNCYKVGVKVGSLTKYVIASPVHLKRIKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-03" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C04</accession>
    <name>GCSPB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable glycine dehydrogenase (decarboxylating) subunit 2</fullName>
            <ecNumber evidence="1">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycine cleavage system P-protein subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine decarboxylase subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine dehydrogenase (aminomethyl-transferring) subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvPB</name>
        <name type="ordered locus">TV0301</name>
        <name type="ORF">TVG0311358</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvP family. C-terminal subunit subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916556.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C04"/>
    <dbReference type="SMR" id="Q97C04"/>
    <dbReference type="STRING" id="273116.gene:9381075"/>
    <dbReference type="PaxDb" id="273116-14324516"/>
    <dbReference type="GeneID" id="1440814"/>
    <dbReference type="KEGG" id="tvo:TVG0311358"/>
    <dbReference type="eggNOG" id="arCOG00076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_5_0_2"/>
    <dbReference type="OrthoDB" id="371967at2157"/>
    <dbReference type="PhylomeDB" id="Q97C04"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000224">
        <property type="entry name" value="Probable glycine dehydrogenase (decarboxylating) subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00713">
        <property type="entry name" value="GcvPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023012">
        <property type="entry name" value="GcvPB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003346">
        <property type="entry name" value="PRK04366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000167034" description="Probable glycine dehydrogenase (decarboxylating) subunit 2">
        <location>
            <begin position="1"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00713"/>
        </source>
    </evidence>
    <sequence length="472" mass="52790" checksum="92977275D2E74AE7" modified="2001-10-01" version="1">MNFKQAYYEEPIIKDIKSSNTFSLSEQVDESILPENLKRKDLELPEVSEYDVVRHYTRLSQMNYTVDVGIYPLGSCTMKYNPKFADRVSAIDGFRNIHPFQPENTVQGALHVMYDLQEYLKKISDMDAVSLQPMAGADGEFTGILIVKKYFEDKGEDRTEIIIPDSAHGTNPASATMGGFDVVEVPSDDKGMVDLEALRAAVSKKTAAFMITNPNTLGIFEQNIEEIAKIIHNAGALLYYDGANLNAIFGITSPGLMGFDIVHFNLHKSFATPHGGGGPGAGPVAVKSFLKDFLPVPIVDFDGNSYRLNYELKKTIGKVSSFYGSFSILLRAWSYIIRNGDDGLKNVSARAVLNSNYLKKKLEKYYDIPYYPLKKHEFVLSTENTGKRALDIGKYILDNGIHSPTVYFPLIVKEAMMIEPTETVSKADLDNYADVLIEALKLSDEELKSRPKNTAVRRIDEVKAARDLKLKW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C60</accession>
    <name>FDHD_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfur carrier protein FdhD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fdhD</name>
        <name type="ordered locus">TV0245</name>
        <name type="ORF">TVG0258796</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for formate dehydrogenase (FDH) activity. Acts as a sulfur carrier protein that transfers sulfur from IscS to the molybdenum cofactor prior to its insertion into FDH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FdhD family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916501.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C60"/>
    <dbReference type="SMR" id="Q97C60"/>
    <dbReference type="STRING" id="273116.gene:9381016"/>
    <dbReference type="PaxDb" id="273116-14324459"/>
    <dbReference type="GeneID" id="1440761"/>
    <dbReference type="KEGG" id="tvo:TVG0258796"/>
    <dbReference type="eggNOG" id="arCOG04358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056887_3_0_2"/>
    <dbReference type="OrthoDB" id="57189at2157"/>
    <dbReference type="PhylomeDB" id="Q97C60"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097163">
        <property type="term" value="F:sulfur carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00187">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00129">
        <property type="entry name" value="fdhD_narQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="FORMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592:SF1">
        <property type="entry name" value="SULFUR CARRIER PROTEIN FDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152943" description="Sulfur carrier protein FdhD">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00187"/>
        </source>
    </evidence>
    <sequence length="279" mass="30327" checksum="3CCEAFA596AC25B2" modified="2001-10-01" version="1">MKAATHLNGLTSHRTLKRIDSYGWLEAEDQVAQEEPLEIKFRIGSDKEKSAGIVMRTPVHDDWLAVGFLYSEGILHDRRWINGIEGVSIEGKAQDNIVTVNIDPRNGSFDPDYRERFVNSSCGICGKSTINGIFLRMGKIKRDNIKIDPGVIVSLPVIMKGMQQLFSKTGGIHAAALFSADGKPIVTAEDIGRHNAVDKVVGFSLIEGIDTSTLILQVSGRAGFEIVEKAAIAGIPVICSVSAPSSLAIEVCESLGLTLICFVRGSSFNVYTHAERIMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C63</accession>
    <name>Q97C63_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0252968 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0252968</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916496.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C63"/>
    <dbReference type="PaxDb" id="273116-14324456"/>
    <dbReference type="GeneID" id="1440756"/>
    <dbReference type="KEGG" id="tvo:TVG0252968"/>
    <dbReference type="eggNOG" id="arCOG04394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127515_4_1_2"/>
    <dbReference type="OrthoDB" id="55761at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="105" mass="11573" checksum="1C508323D8DB5BEC" modified="2001-10-01" version="1">MKIILSVDSEEKIPMSITAATHLSEMPETELVEVVYLNGGIAAVTEKNRIGPILKNKKVQVVACGTSMEARNIQKDELAPGVVYVPASLKEIIKRKQEGYMYLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C66</accession>
    <name>Q97C66_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0247983</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916492.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C66"/>
    <dbReference type="STRING" id="273116.gene:9381009"/>
    <dbReference type="PaxDb" id="273116-14324452"/>
    <dbReference type="GeneID" id="1440752"/>
    <dbReference type="KEGG" id="tvo:TVG0247983"/>
    <dbReference type="eggNOG" id="arCOG02274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154393_0_0_2"/>
    <dbReference type="OrthoDB" id="56014at2157"/>
    <dbReference type="PhylomeDB" id="Q97C66"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056531">
        <property type="entry name" value="HVO_A0563_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24280">
        <property type="entry name" value="HVO_A0563_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HVO-A0563 N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="163"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="232" mass="26831" checksum="39C380E873142885" modified="2001-10-01" version="1">MDEIEMDLENDLPFCKLSKDFPQVSFYRWCNSAIDYLEFYGNKEDLDSLEKYVPEIEKSLNTNSLYRSRSGGRLSVILSCRCNVSNSTIRKAEHHNLMWKAPVFYNGGHEKLSVISPRSEDFLGLFKDLEDVGKVRITKKIEVDPDLVRDIYSISLSDLFSDLTEKQLRYLVSAYHNGYFDVPKRTNIVELAKQFNISYSTLQEHLEKAVNKIMKGIEPYLTIALQMMGGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CA2</accession>
    <name>Q97CA2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alanyl-transfer RNA synthetases family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0206628</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916457.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CA2"/>
    <dbReference type="STRING" id="273116.gene:9380971"/>
    <dbReference type="PaxDb" id="273116-14324415"/>
    <dbReference type="GeneID" id="1440717"/>
    <dbReference type="KEGG" id="tvo:TVG0206628"/>
    <dbReference type="eggNOG" id="arCOG01254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_3_2_2"/>
    <dbReference type="OrthoDB" id="11392at2157"/>
    <dbReference type="PhylomeDB" id="Q97CA2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000010">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053424">
        <property type="entry name" value="Alanyl-tRNA_Edit-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051335">
        <property type="entry name" value="Alanyl-tRNA_Editing_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040865">
        <property type="entry name" value="a_tRNA_ed_AlaXM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462:SF1">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN AARSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="242" mass="27469" checksum="7164DA9AA1BA0149" modified="2001-10-01" version="1">MTEKLYYADSYIKEFDGNVIEVKENRLILDRTAFYPTGGGQPNDTGVIFCCGKEYRVNDVTKDGDEVIHVVDDSTGLSAGAKVHGTIDWDRRYAHMRYHTAIHVIDGVVNVMHGSEALLTGGQIYEDRARIDMNIENFTKEFVDKIIEESNSFMKEGHRVYPDILTKEEAIKRPNLARTEPGRKLIETLPEVRIIVIEGLDEQSDGGTHVSNTKEVGTIFVRKIENKGKRNKRIEFQLIPPD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-16" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CA8</accession>
    <name>AROD_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="1">3-dehydroquinase</shortName>
            <ecNumber evidence="1">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Type I DHQase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Type I dehydroquinase</fullName>
            <shortName evidence="1">DHQ1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroD</name>
        <name type="ordered locus">TV0194</name>
        <name type="ORF">TVG0199907</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916450.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CA8"/>
    <dbReference type="SMR" id="Q97CA8"/>
    <dbReference type="STRING" id="273116.gene:9380964"/>
    <dbReference type="PaxDb" id="273116-14324408"/>
    <dbReference type="GeneID" id="1441680"/>
    <dbReference type="KEGG" id="tvo:TVG0199907"/>
    <dbReference type="eggNOG" id="arCOG02097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1212642_0_0_2"/>
    <dbReference type="OrthoDB" id="56320at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046279">
        <property type="term" value="P:3,4-dihydroxybenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00214">
        <property type="entry name" value="AroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050146">
        <property type="entry name" value="Type-I_3-dehydroquinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002321">
        <property type="entry name" value="PRK01261.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000138841" description="3-dehydroquinate dehydratase">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00214"/>
        </source>
    </evidence>
    <sequence length="230" mass="26638" checksum="2986B54CC5DC6126" modified="2001-10-01" version="1">MPIIKSSRVNIGRFTIGGSIPIVIISIFDHDAKSLTEKFETKKLSDKNLYEIRFDLFDKVNIDEELEIIRALDEMDIDYIFTYRGHDPEKIYETAITKMVPAVDMDLSLLNKVNRRSPDTRIMVSYHTDSSKDMIEKLDEMLKANADIVKVACNYKDEKNFFKDLIEIAQRKEISGKPVLFIPMGKSFLRVISAYYVSDMVYAKYDKETAMGQPDPDYYNKAFSLFNYIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CC8</accession>
    <name>KTHY_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable thymidylate kinase</fullName>
            <ecNumber evidence="1">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmk</name>
        <name type="ordered locus">TV0174</name>
        <name type="ORF">TVG0181531</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dTMP + ATP = dTDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916429.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CC8"/>
    <dbReference type="SMR" id="Q97CC8"/>
    <dbReference type="STRING" id="273116.gene:9380944"/>
    <dbReference type="PaxDb" id="273116-14324388"/>
    <dbReference type="GeneID" id="1441659"/>
    <dbReference type="KEGG" id="tvo:TVG0181531"/>
    <dbReference type="eggNOG" id="arCOG01891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_1_3_2"/>
    <dbReference type="OrthoDB" id="43083at2157"/>
    <dbReference type="PhylomeDB" id="Q97CC8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF4">
        <property type="entry name" value="UMP-CMP KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155403" description="Probable thymidylate kinase">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <sequence length="190" mass="21863" checksum="C0E7A2A4AE737145" modified="2001-10-01" version="1">MFIAVEGIDGAGKTTLAKSLSSLLEKEGFRVFLTREPTDDIRNYEGDDVELFIKFTLDRYKHQKEIRKKLNEGFVVISDRYIRSSYAYEMKGAAAALGSEEKAKEWMDCVSNIITIRPDINILVQVDVQVGLDRISKRNGTITHFEQRERLNEALKIYNSFDWDIKVDGTDPLDKITNEVYLYLKNKIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CC9</accession>
    <name>Q97CC9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0181068 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0181068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916428.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CC9"/>
    <dbReference type="STRING" id="273116.gene:9380943"/>
    <dbReference type="PaxDb" id="273116-14324387"/>
    <dbReference type="GeneID" id="1441658"/>
    <dbReference type="KEGG" id="tvo:TVG0181068"/>
    <dbReference type="HOGENOM" id="CLU_046006_18_5_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="126" mass="13924" checksum="785BEAEF22F63A42" modified="2001-10-01" version="1">MKVTGIGWLGYEVRTGESDYLKAVDLFKSFFGIEPSEFDRESALFVLPNGDEFEVKNGASGSDNACNREVAGFLVDDIPSGIKRLNNIGVSDIDSLECGSDGSCWQHFRLPDGTEFELKSYSRVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CD3</accession>
    <name>Q97CD3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-dehydro-3-deoxyphosphogalactonate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0179275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916424.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CD3"/>
    <dbReference type="STRING" id="273116.gene:9380939"/>
    <dbReference type="PaxDb" id="273116-14324383"/>
    <dbReference type="DNASU" id="1441654"/>
    <dbReference type="GeneID" id="1441654"/>
    <dbReference type="KEGG" id="tvo:TVG0179275"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_3_2_2"/>
    <dbReference type="OrthoDB" id="42605at2157"/>
    <dbReference type="PhylomeDB" id="Q97CD3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03316">
        <property type="entry name" value="MR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="DgoD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080:SF2">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="2">
        <location>
            <begin position="142"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="398" mass="44650" checksum="902DFB8C40D22E64" modified="2001-10-01" version="1">MNSAIKDIEIIEMGEKLRETSSPWSSTILLVKLTSADGLVGYGEAPTTLMTLPVLESMKEVARVFKGKDFFNVEQNVFDFYRNSFYLPVSMEATSALSAFEIASWDLIGKELGTPVYKLIGGEVNHNIRAYANGWYNDCVTPDDFVKKAKEVVKMGFTAMKFDPFGDNYDTITNEGIENARNIVGALRSEFGEKVDLLIEYHGRFSKIAAIKAGMALEEFKPYFYEEPLHPDLDHDLLDLKQYYKVPIALGERVLNKKIFARFISQKLVDIIQPDLTNSGGILEAKKIAAIADGFGIPIAPHNAFGPIQTAATLNVDTTLTNFAIQESFEMFWPDWKKNLMASGYKLENGYFRLTGDAGIGKVNESIVEKYQVSGMEPFDDKEPPWVVSGTFKHYQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CD6</accession>
    <name>Q97CD6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0175536</name>
    </gene>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Thermoplasmata</taxon>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="305" mass="33435" checksum="A1BA9CB7D27BB755" modified="2001-10-01" version="1">MPSIEIKDVSKYYGKFRALDGVSFKYDGEGAIGYLGPNGAGKTTTLKILTNLIKPSTGVALINGINVNKEPVKAFAVTGSVIESPSPFPYFTIMDSLMFVADIRKIDHREAKKSIDEYADILKLPPLDSKIGDLSKGQRQRVVIAAALMPDPEILLLDEPTSGLDPFEMKIIRDLIKAYKKDKLILMSSHLLSEVSEVCDDVIFINHGKILATDKVSNLSNQFRSKKLIAETLQPITDDIIAKIKKAGIDAEKSGENSLVISYDGSNERRADILKKLVDIAPVISYESAGSELEQAYISILSESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CF0</accession>
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            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Thermoplasmata</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59293.1"/>
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    <dbReference type="AlphaFoldDB" id="Q97CF0"/>
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    <dbReference type="GeneID" id="1441636"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="469" mass="52417" checksum="9598A624316234AE" modified="2001-10-01" version="1">MNFRLIRFVIKTRFTTPMLVFYIFLIILLLYDSYLSYTSNLVLRANSIYLDGIISLVIFIISISGSVMLKKSDVDFLFQLPVKRSDLGIALLFSSLLSFSITPIFLGFLVLTVMSGYSIFIGILDIFLQVFLAVEIGFLIRNFNFRKRAIISAILAIWFLLPGLSYFDLAPTITYSGYPFVGTIVLIALLVPISYLAISRAGNATYLFSSSFVEKMGEVSHGISFYGLSPKSAVRKRYFMISGSSGVMRVGAAARSYTSRGGIRSFFIVSVVIAILYGILIYFIPKSMYLFVVPYVVLYGVFIIPTARSYGSVSSERVWLAFMSMPPHIYLRNASLARSFSQLLLSVPFFAANILLFIILKANIFLAASSYFVLISPAISIISFFMSAYINPMQIIDIGEISYSYRNSLSSLLTMLILLAISAPAIIGLFTPIFSVYYVVGIWIPSAIILYLPRTGSAIMKRMVERNFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CF1</accession>
    <name>Q97CF1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transport system ATP-binding protein</fullName>
        </submittedName>
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    <gene>
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    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
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    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59292.1"/>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
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    <dbReference type="STRING" id="273116.gene:9380920"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="87732at2157"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
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        <property type="entry name" value="ABC_transporter_ATP-binding"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
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        <property type="entry name" value="AAA"/>
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    <dbReference type="SUPFAM" id="SSF52540">
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    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="239" mass="26578" checksum="2326AD33A19FD1ED" modified="2001-10-01" version="1">MECIHFEQVSKYYGKKRAIDGLTFRLDCGESMALIGPNGAGKSTTLKILAGILKPDSGSVSISGYDPSTNDARKIVGYLPEDAAPYFTLSVRENLEYIGALRGVQNLNSRMDFLMELLGLGNYRNVKVSALSRGNRQKLAIALVLIHDPKVLLLDEPLNYLDIPSQEAITKYFHSLKATFLVSTHIMSIAERFTSRILIINNGRKVWEGDIQQIKDQSNRSNVSVEDLITRIMGGQLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CG0</accession>
    <name>Q97CG0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0147974 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0147974</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CG0"/>
    <dbReference type="PaxDb" id="273116-14324355"/>
    <dbReference type="KEGG" id="tvo:TVG0147974"/>
    <dbReference type="HOGENOM" id="CLU_2140341_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="112" mass="13559" checksum="510DC527B0E953D7" modified="2001-10-01" version="1">MLYHRIVERKHLKYFVLFFHGNNSTCQDTRTVQEQHEETEEQGSSIFRKLYGYDMHSNYNQYNKRKKGLLEEIPSIRYEKGMIMIRETDFEKVKSLIDSRTKIVIHLPHHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CH1</accession>
    <name>Q97CH1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Potassium channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0139547</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916384.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CH1"/>
    <dbReference type="STRING" id="273116.gene:9380899"/>
    <dbReference type="PaxDb" id="273116-14324344"/>
    <dbReference type="GeneID" id="1441614"/>
    <dbReference type="KEGG" id="tvo:TVG0139547"/>
    <dbReference type="eggNOG" id="arCOG01617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CH1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19091">
        <property type="entry name" value="AKR_PsAKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF4">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="339" mass="38808" checksum="63625506AC3849F8" modified="2001-10-01" version="1">MKYVRYGSTASFVSVLSFGAMTFGERNRWKLGGVTQELSDRMVKRAYDAGINLFDTADVYDDGDSEVMLGRSLKPYRDSVMIATKVRGKIGQGVNSSGLSRHHMHIAIKKSLERLGTEWIDIYQYHGWDVYGNFEEFLETMESFVEQGLVIYPAVSNFTAWQISVLQTMAIERGYARYESAQMNYSLLNRDVEYEVLPFMKYSGMTLLSWSPLHGGVLAGKYKKGEKPATGTRMGNRGFFFPYFDEDHGWDVVEEVKKVAQEQGCKPSQVALAWIISKSHVAILGARSMEQLEENLGSLDVQLTKEQMDRLDNVSKNREIYPNWMVERQNHERDFEIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CH5</accession>
    <name>Q97CH5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Modification methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0133675</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916380.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CH5"/>
    <dbReference type="SMR" id="Q97CH5"/>
    <dbReference type="STRING" id="273116.gene:9380894"/>
    <dbReference type="REBASE" id="685114">
        <property type="entry name" value="M.TvoORF3675P"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324340"/>
    <dbReference type="GeneID" id="71201306"/>
    <dbReference type="KEGG" id="tvo:TVG0133675"/>
    <dbReference type="eggNOG" id="arCOG00108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822885_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CH5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="4">
        <location>
            <begin position="106"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="337" mass="39254" checksum="9603FD3F3BE679CE" modified="2001-10-01" version="1">MIEFSENSHRKSLIEELPRIVERGEKEAQKILDGLSKSQRITLQTNELVLPTKAIGELNKFYGQTIKTENNNEFLNRLIYGDNLLAMQAFLAGDPETGLPSMRGKIDLIYIDPPFDSKANYRTKIHLPTADVEQMPSVIEQFAYSDTWKDGTKSYLEMIVPRLVLMKELLSEKGSIYVHIDWHVGHYVKVIMDEIFGRENFVNEIVWKKTNSPKAQSKGFGTQHDVLYIYRKTPQFIFNQIKKEPDEDYLKSYVYDDNDGRGPYQTVALVAGGIQRSENRKIFEFHGVRAPWLYSLENLERFWSDGRIFKTKTGYRLKVYLKDIALRLYLIYGLTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CJ4</accession>
    <name>Q97CJ4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system Cms protein Csm5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CRISPR type III A-associated protein Csm5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0112611</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This subunit might be involved in maturation of a crRNA intermediate to its mature form.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm5 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916364.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CJ4"/>
    <dbReference type="STRING" id="273116.gene:9380875"/>
    <dbReference type="PaxDb" id="273116-14324321"/>
    <dbReference type="GeneID" id="1441594"/>
    <dbReference type="KEGG" id="tvo:TVG0112611"/>
    <dbReference type="eggNOG" id="arCOG03720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036878_3_0_2"/>
    <dbReference type="OrthoDB" id="86248at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09662">
        <property type="entry name" value="Csm5_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010173">
        <property type="entry name" value="CRISPR-assoc_Csm5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01899">
        <property type="entry name" value="cas_TM1807_csm5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38007">
        <property type="entry name" value="CRISPR SYSTEM CMS PROTEIN CSM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38007:SF1">
        <property type="entry name" value="CRISPR SYSTEM CMS PROTEIN CSM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="7">
        <location>
            <begin position="5"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="385" mass="45032" checksum="64A37FE6B29CC0FB" modified="2001-10-01" version="1">MEVQLKVSSPVLIGNDYVNVTNMIINKDSLYMLDMDKLIDNEPKIDQLAEYFLSYKSSDKDEYSKYNEIKRFYNNINAENYKIEPEIKGDKEFYEVINSNDNKFLKAKDVKLTMGTYEVINNKKTFVPFIPGSTIKGAIRNAIRNEYIKKNKINITYDDHKKVKSYNFNGNSYGDLDEAIFYKEDQKRQNEPKKISNDVFRFVEVDDFYPKEYSLKIYRIERIKLSNNKRNGIPFYAISIDSGTFTGNIKIGRSFDNIYKSDKNQFYAISKVFNKLIDIKINDASDHTKNEIINKMLCISGKYYRDILKKEKEHYNDNPDKEVLLIGFGGGIEEKTIINSLDDEQFKKAEHIIKISNKKIKFSGRMPSTAWTINNKKFGVLEVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CK0</accession>
    <name>Q97CK0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">2-oxoisovalerate dehydrogenase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0106097</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916358.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CK0"/>
    <dbReference type="STRING" id="273116.gene:9380869"/>
    <dbReference type="PaxDb" id="273116-14324315"/>
    <dbReference type="GeneID" id="1441588"/>
    <dbReference type="KEGG" id="tvo:TVG0106097"/>
    <dbReference type="eggNOG" id="arCOG01054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_1_0_2"/>
    <dbReference type="OrthoDB" id="25266at2157"/>
    <dbReference type="PhylomeDB" id="Q97CK0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016624">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="3">
        <location>
            <begin position="19"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="337" mass="37703" checksum="4D04BE7FC60A1061" modified="2001-10-01" version="1">MTEVLDQDQKSLYVKGFTSMVLGRLFDKKVITAQRQGLVGFYTPMMGQEATQAGAAMALSKQDLVYGYYRDVTLLIYLGYPIEKIFDQIMGNAEDTSKGRQMPSHYSAKAVNFMSVPSPVATNLPLAVGAAYAKKYRKEDGIVITSFGDGGTSTPDFHAAMNFASVYDLPVVFLCENNGWAISFPVERQTKAEIYKKAEAYGMKGVYVDGNDFIKTYNAVKEAVEYARSGNPILVEARSYRMGPHSTSDDPSKYRQNEVKEGDENDPLVIAEKAVISKGILSQSEVNRIKDESRKMIDEKFEERLKIPAPDPSTLFDDVYSETTWVIEEERGDILRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CL4</accession>
    <name>Q97CL4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0089391</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916344.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CL4"/>
    <dbReference type="STRING" id="273116.gene:9380855"/>
    <dbReference type="PaxDb" id="273116-14324301"/>
    <dbReference type="DNASU" id="1441574"/>
    <dbReference type="GeneID" id="1441574"/>
    <dbReference type="KEGG" id="tvo:TVG0089391"/>
    <dbReference type="eggNOG" id="arCOG00585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_2"/>
    <dbReference type="OrthoDB" id="8297at2157"/>
    <dbReference type="PhylomeDB" id="Q97CL4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001119">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="284" mass="30197" checksum="8BFDF61E9197DB20" modified="2001-10-01" version="1">MAKGTIKTNTPPPAPVVGPPGKSAKYRVKHTITVMSGKGGVGKSTVAVNLAVSLAKKGLKVGLIDADINGPDDPKLLGVEDLKLYADDDGIIPAETKYGVKVVSMGFLLPSQDTPVIWRGSLMHKAIQQFLEDVSWKDTDYVVLDMPPGTGDVALSVAQLVPESNGVVIVVTPQDVALLDAKKAINFARQLKLPIFGIIENMSGFVCPHCGNVTYIFKEGGGEKSAKEYNVPFLGKIPLVPEIADNGDKGIPAVEINDTIKSIFNDIADKILKQILGENVKTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CP2</accession>
    <name>Q97CP2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Superoxide dismutase</fullName>
            <ecNumber evidence="2 6">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0062871</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916317.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CP2"/>
    <dbReference type="SMR" id="Q97CP2"/>
    <dbReference type="STRING" id="273116.gene:9380824"/>
    <dbReference type="PaxDb" id="273116-14324273"/>
    <dbReference type="GeneID" id="1441547"/>
    <dbReference type="KEGG" id="tvo:TVG0062871"/>
    <dbReference type="eggNOG" id="arCOG04147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031625_2_2_2"/>
    <dbReference type="OrthoDB" id="32917at2157"/>
    <dbReference type="PhylomeDB" id="Q97CP2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050265">
        <property type="entry name" value="Fe/Mn_Superoxide_Dismutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404">
        <property type="entry name" value="SUPEROXIDE DISMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404:SF6">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [MN], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="7">
        <location>
            <begin position="19"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="8">
        <location>
            <begin position="95"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="205" mass="23797" checksum="988E8C9190B1CD7D" modified="2001-10-01" version="1">MAETWEIKEKLKPRGLEGISDVQIDNHFDVHYKGYVNKLNEIWSRLPDVDRSKANQNYSEFRALKLEETFNYGGSFLHELYFEGLTPSHSEVPDEFKDAVAKDFGSYEKWLEDFKATGTAFRGWAILVFDLNYGKLRNIGSDAHNVGLIWNSIAILTMDVYEHAYYVDYGAKRAPYLDAFMKNVNWPVVLKRLEKAKKAYEAFKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-08" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CP8</accession>
    <name>AATD_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpD</name>
        <name type="ordered locus">TV0053</name>
        <name type="ORF">TVG0056269</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760290.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CP8"/>
    <dbReference type="SMR" id="Q97CP8"/>
    <dbReference type="STRING" id="273116.gene:9380818"/>
    <dbReference type="PaxDb" id="273116-14324267"/>
    <dbReference type="GeneID" id="1441540"/>
    <dbReference type="KEGG" id="tvo:TVG0056269"/>
    <dbReference type="eggNOG" id="arCOG04101">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069688_2_1_2"/>
    <dbReference type="PhylomeDB" id="Q97CP8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001545">
        <property type="entry name" value="PRK00373.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144262" description="A-type ATP synthase subunit D">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00271"/>
        </source>
    </evidence>
    <sequence length="209" mass="24441" checksum="D11E08C5435BBE9D" modified="2001-10-01" version="1">MEIRPTRIELIRTRRRIKLARKGLDLLKMKRSALIYEFLQISRTIRGMRENLRREVEDALNTIRTAEILEGQVALERIANMSSDSTINVDSRNVMGVVIPTLNLTYNLSILSDVYRTISVPVAINDAIDRFQRLFLNLIQILEKENALRNLLIEIDKTKRRSNAIENILIPRLEYQAKMIKMMLDERERDTFTTLKTIKKKIEAGKDEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CQ1</accession>
    <name>AATF_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpF</name>
        <name type="ordered locus">TV0050</name>
        <name type="ORF">TVG0051934</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916307.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CQ1"/>
    <dbReference type="SMR" id="Q97CQ1"/>
    <dbReference type="STRING" id="273116.gene:9380815"/>
    <dbReference type="PaxDb" id="273116-14324264"/>
    <dbReference type="GeneID" id="1441537"/>
    <dbReference type="KEGG" id="tvo:TVG0051934"/>
    <dbReference type="eggNOG" id="arCOG04102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313886_0_0_2"/>
    <dbReference type="OrthoDB" id="24971at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00312">
        <property type="entry name" value="ATP_synth_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022944">
        <property type="entry name" value="ATPase_V1-cplx_fsu_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002256">
        <property type="entry name" value="PRK01189.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144826" description="A-type ATP synthase subunit F">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00312"/>
        </source>
    </evidence>
    <sequence length="104" mass="11587" checksum="C791A72A565CF3F0" modified="2001-10-01" version="1">MQSCITVIGERDVVLGFRLLGITNTIVAEGKDLVKKFMEEFENPHCSVIVVSEHLKNMIDKKTLRSIEVSSKPLVVFIPLPGFKEEESIETMAKRILGIDIGSV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-10" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CR6</accession>
    <name>DP2L_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>DNA polymerase II large subunit</fullName>
            <shortName>Pol II</shortName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Exodeoxyribonuclease large subunit</fullName>
            <ecNumber evidence="2">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">polC</name>
        <name type="ordered locus">TV0035</name>
        <name type="ORF">TVG0041370</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal DNA polymerase II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EC" id="3.1.11.1" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CR6"/>
    <dbReference type="SMR" id="Q97CR6"/>
    <dbReference type="STRING" id="273116.gene:9380800"/>
    <dbReference type="PaxDb" id="273116-14324249"/>
    <dbReference type="KEGG" id="tvo:TVG0041370"/>
    <dbReference type="eggNOG" id="arCOG04447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001154_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CR6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00324">
        <property type="entry name" value="DNApol_II_L_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004475">
        <property type="entry name" value="PolC_DP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056172">
        <property type="entry name" value="PolC_DP2_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056171">
        <property type="entry name" value="PolC_DP2_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016033">
        <property type="entry name" value="PolC_DP2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00354">
        <property type="entry name" value="polC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003103">
        <property type="entry name" value="PRK04023.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210:SF1">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24846">
        <property type="entry name" value="PolC_DP2_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24844">
        <property type="entry name" value="PolC_DP2_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03833">
        <property type="entry name" value="PolC_DP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016275">
        <property type="entry name" value="PolC_DP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000152581" description="DNA polymerase II large subunit">
        <location>
            <begin position="1"/>
            <end position="1088"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1088" mass="122362" checksum="62B550A0A9BE7892" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CS2</accession>
    <accession>B7VF69</accession>
    <name>Q97CS2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Proteinase IV</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7">Signal peptide peptidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sspA</name>
        <name evidence="6" type="ORF">TVG0029868</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
            <strain evidence="6">GSS1</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="journal article" date="2009" name="Biotechnol. J." volume="4" first="1055" last="1065">
            <title>Cloning and overexpression of a thermostable signal peptide peptidase (SppA) from Thermoplasma volcanium GSS1 in E. coli.</title>
            <authorList>
                <person name="Kocabiyik S."/>
                <person name="Demirok B."/>
            </authorList>
            <dbReference type="PubMed" id="19557795"/>
            <dbReference type="DOI" id="10.1002/biot.200800344"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Type strain:4299</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="FM878943">
        <property type="protein sequence ID" value="CAT00893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916286.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CS2"/>
    <dbReference type="SMR" id="Q97CS2"/>
    <dbReference type="STRING" id="273116.gene:9380794"/>
    <dbReference type="MEROPS" id="S49.007"/>
    <dbReference type="PaxDb" id="273116-14324243"/>
    <dbReference type="DNASU" id="1441515"/>
    <dbReference type="GeneID" id="1441515"/>
    <dbReference type="KEGG" id="tvo:TVG0029868"/>
    <dbReference type="eggNOG" id="arCOG01311">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046540_0_0_2"/>
    <dbReference type="OrthoDB" id="31107at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004635">
        <property type="entry name" value="Pept_S49_SppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047272">
        <property type="entry name" value="S49_SppA_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00706">
        <property type="entry name" value="SppA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42987">
        <property type="entry name" value="PEPTIDASE S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42987:SF4">
        <property type="entry name" value="PROTEASE SOHB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S49" evidence="5">
        <location>
            <begin position="60"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAT00893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="230" mass="25586" checksum="FA1B6F36DB0DDB24" modified="2001-10-01" version="1">MYLLRINIEGTINYGLYKYLYPALKAAEGKKSIAGLILVFNSGGGDAASSQLIHDLVKKIRKKKPVYSLALGICASGAYWIASASTKIYAIDTSLIGSIGVISIRPNVKKLMEKIGVDVMVYKSGKYKDMTSPFSEPNEEEKSVYQRLLDDIFEKFKRSVAEDRGIPSEKIDEIANGMVYSAKMAADNGLIDRIANYDDLVSDLTKEVGKRLKVKEFYIRKPLLQRLLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CS6</accession>
    <name>Q97CS6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0025269 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0025269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CS6"/>
    <dbReference type="STRING" id="273116.gene:9380790"/>
    <dbReference type="PaxDb" id="273116-14324239"/>
    <dbReference type="DNASU" id="1441511"/>
    <dbReference type="KEGG" id="tvo:TVG0025269"/>
    <dbReference type="eggNOG" id="arCOG00919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536742_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="174" mass="19470" checksum="7BED70113547CA3E" modified="2001-10-01" version="1">MGYKMNGSVYEREIRDILSGRAQAVEKYAQRVGENGSTVRSLITSPFFVTRAAGSLGADIIAIRHDISMVIEVKSSIRSYLAFSEASGKKQDQAERLSEMVNRAGIFLVYAFRLKNASGDPWRLFTVPGNPTGRMKTLYELIPTLYKTKDGNYVMKWDNGLPFTEFIAIINGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CU5</accession>
    <name>Q97CU5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cas12f1-like TNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0007048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916263.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CU5"/>
    <dbReference type="STRING" id="273116.gene:9380771"/>
    <dbReference type="PaxDb" id="273116-14324220"/>
    <dbReference type="DNASU" id="1441491"/>
    <dbReference type="GeneID" id="1441491"/>
    <dbReference type="KEGG" id="tvo:TVG0007048"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881184_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CU5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="3">
        <location>
            <begin position="30"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="135" mass="15046" checksum="6D929D24AD48332A" modified="2001-10-01" version="1">MRIGGINQITKKGDGKGKDYRFLLKNAFPYGMLASQVMYKASWEGLPAIELTRKETRNTSRMCSVCDTLARIGHGRILKCDSCGIEIDRDVNACINIAFRYRTCLKRSHKGLPGEAVKQSKDVEQMAGSQISTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q978C2</accession>
    <name>Q978C2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TVG1548359 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1548359</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917724.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978C2"/>
    <dbReference type="STRING" id="273116.gene:9382309"/>
    <dbReference type="PaxDb" id="273116-14325734"/>
    <dbReference type="GeneID" id="1442184"/>
    <dbReference type="KEGG" id="tvo:TVG1548359"/>
    <dbReference type="eggNOG" id="arCOG04918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2911751_0_0_2"/>
    <dbReference type="OrthoDB" id="56934at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="61" mass="7561" checksum="72FA8D8E41B6F4BE" modified="2001-10-01" version="1">MNKEIRRRIKIIDSLPSEESAMKIIYLRSTEIDEKWSQRSLRGFYKCRDEIMEMFQMRYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q978E3</accession>
    <name>Q978E3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
            <shortName evidence="1">HPPK</shortName>
            <ecNumber evidence="1">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">6-hydroxymethyl-7,8-dihydropterin diphosphokinase</fullName>
            <shortName evidence="1">6-HMPDK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">7,8-dihydro-6-hydroxymethylpterin diphosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase</fullName>
            <shortName evidence="1">PPPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mptE</name>
        <name evidence="3" type="ORF">TVG1525771</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of diphosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (6-HMD), leading to 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal 6-HMPDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978E3"/>
    <dbReference type="STRING" id="273116.gene:9382288"/>
    <dbReference type="PaxDb" id="273116-14325713"/>
    <dbReference type="KEGG" id="tvo:TVG1525771"/>
    <dbReference type="eggNOG" id="arCOG04303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093043_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978E3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02131">
        <property type="entry name" value="HMPDK_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027510">
        <property type="entry name" value="HMPDK_MptE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001162">
        <property type="entry name" value="UvrC_RNase_H_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648:SF1">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MptE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50165">
        <property type="entry name" value="UVRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UvrC family homology region profile" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="219" mass="24653" checksum="B90F526BD678DA22" modified="2001-10-01" version="1">MNLFRWMDIYRAICDDFNINPSSDYISAVILSSLSGEFKLPHRSNEINVVGNGPELKDILTTVTKRFAIVADSAISIYYEELGCPDIIVTDLDGDLSKIWNCKGQGTLIVVHAHGDNIKRIIANKEHIDRRVAGTTQNLPYRQIKNFYGFSDGDRAAYLADYLGAQKIFLIGFDFLKPSAKPGSNLLIKMKKLKWARYLIAELAKSRRSSFNEGEVSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q978E8</accession>
    <name>Q978E8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein transport protein sec61 gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1518472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978E8"/>
    <dbReference type="STRING" id="273116.gene:9382281"/>
    <dbReference type="PaxDb" id="273116-14325708"/>
    <dbReference type="KEGG" id="tvo:TVG1518472"/>
    <dbReference type="eggNOG" id="arCOG02204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2244000_0_0_2"/>
    <dbReference type="OrthoDB" id="52835at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="104" mass="12039" checksum="54F7D2E820A0A885" modified="2001-10-01" version="1">MDHLQVRTQAGVYLYHTFKFPFFIMSCFGDSVAIEDKAEEIQRNIERKFSSIGKGKYARILRMARKPTRDEYIKVLLITAIGLLIIGGVGFIIYLLMDVYFKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q978J2</accession>
    <name>Q978J2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Periplasmic divalent cation tolerance protein [CutA]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1470915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917655.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978J2"/>
    <dbReference type="PaxDb" id="273116-14325662"/>
    <dbReference type="GeneID" id="1442113"/>
    <dbReference type="KEGG" id="tvo:TVG1470915"/>
    <dbReference type="eggNOG" id="arCOG04231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098807_2_0_2"/>
    <dbReference type="OrthoDB" id="8015at2157"/>
    <dbReference type="PhylomeDB" id="Q978J2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004323">
        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="DIVALENT CATION TOLERANCE CUTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419:SF8">
        <property type="entry name" value="FI09726P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="105" mass="12515" checksum="34BF601C1C281C4B" modified="2001-10-01" version="1">MPFYVITTFQNAEEARRIGMMALEKQMAACFSIIDNVKSTYWWRGNIEESSEVFCVFKTTDDNEPLLSQFIKEMHNYEVPEIASMKMDKINEEYNRWLNDSCRHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q978J8</accession>
    <name>Q978J8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1464820</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917649.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978J8"/>
    <dbReference type="STRING" id="273116.gene:9382226"/>
    <dbReference type="PaxDb" id="273116-14325656"/>
    <dbReference type="GeneID" id="42990962"/>
    <dbReference type="KEGG" id="tvo:TVG1464820"/>
    <dbReference type="eggNOG" id="arCOG04236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_48_1_2"/>
    <dbReference type="OrthoDB" id="18492at2157"/>
    <dbReference type="PhylomeDB" id="Q978J8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01978">
        <property type="entry name" value="sufC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="3"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="246" mass="27123" checksum="ABD8B4B127B9FDB6" modified="2001-10-01" version="1">MILEINNLSASVEGKEILKNVNLTVKGGEIHALMGPNGAGKSTLGNVLIGHPDYKVTGGNVLLDGKDITFATPEERARAGLFLAFQSPVAVEGVRISTFLRIAYSNLHPGEKIVISEFNKKVKSLMKTVGLDESFISRSVNDGFSGGERKRFEILQMMILKPKIIILDEIDSGLDVDALRMVAEQIRSYLNNDVGFLIITHYQRILKDIPPQFVHVLVNGTIVMNGDKSLSDKIEEEGYDWLKEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q978M4</accession>
    <name>Q978M4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1438442</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917624.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978M4"/>
    <dbReference type="STRING" id="273116.gene:9382199"/>
    <dbReference type="PaxDb" id="273116-14325630"/>
    <dbReference type="GeneID" id="1442082"/>
    <dbReference type="KEGG" id="tvo:TVG1438442"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="PhylomeDB" id="Q978M4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17321">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000503">
        <property type="entry name" value="Drug resistance transporter, EmrB/QacA subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000021">
        <property type="entry name" value="Drug resistance transporter, EmrB/QacA subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="9"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="480" mass="51115" checksum="91C0F6EF504E62AB" modified="2001-10-01" version="1">MALRKEDLILIPLVAGVMMGAIDSTIVVLALPTISDDLKAPLSLSIWIILAYLLVAAVSTTQFGRLGDMISRKTIFNSGMAIFTLGSFLCGVSSNIDFLIAFRFVQAAGGSMMQANSGSIIADNFPPNLRGRAYGYTSVGWNSGATLGIVLGGIITTLIGWRYIFYINVPVGIISLYFAIKYIIPGSKKNVKLDVPGVVTLTAALSSIAYAATDFTSHGLETTNETLLSIGFLLLVVFIFLERTNKNALLPFHLFQNRVFNFSIAASFLQSLGYLAVTFIVIMYLQGLRGLTPLDSSLLLVPGYVLGGFTGPIFGKLSDRIGARTPATLGMFLMMIAVLLYMTFNLKTPVYYVIPVSIISGLGSSMFFPANNSAVMASSPAGSYGAASGLLRTMANIGMLGSFVLAITVSTIAIPRYVAFEVFAGVGRLVGGLSASFLTGIHTALEVSFAIIAVGMLFSFVRGKENRSTSTPPRATETTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q978N2</accession>
    <name>Y1383_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MEMO1 family protein TV1383</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV1383</name>
        <name type="ORF">TVG1430252</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917616.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978N2"/>
    <dbReference type="SMR" id="Q978N2"/>
    <dbReference type="STRING" id="273116.gene:9382191"/>
    <dbReference type="PaxDb" id="273116-14325622"/>
    <dbReference type="GeneID" id="1442074"/>
    <dbReference type="KEGG" id="tvo:TVG1430252"/>
    <dbReference type="eggNOG" id="arCOG01728">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038085_2_0_2"/>
    <dbReference type="OrthoDB" id="372162at2157"/>
    <dbReference type="PhylomeDB" id="Q978N2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04336">
        <property type="entry name" value="AmmeMemoSam_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001987">
        <property type="entry name" value="PRK00782.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000134393" description="MEMO1 family protein TV1383">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00055"/>
        </source>
    </evidence>
    <sequence length="269" mass="29989" checksum="0FEAED53E7DDA5D1" modified="2001-10-01" version="1">MIRKPAVAGYFYPSNRNELLSLISSFHVQQSEVSCQPIGVVVPHAGIVYSGRTAMYSYNALRNSSIRDFIIIGPNHRPMTPYASIFPSGSWETPLGNAIINEELASELYKNSQYIVKDEESHSVEHSIEVQIPFLQYMFGNSFTFVPVILGDQEKVVANDIASALMRLSKPYILIASSDFTHYERSDIVERKDMDLISRIVDLDIDGFYDTIERENVTACGYGAIAILMIIAKKIGAKISLLNHSNSGDVTNDYDEVVGYSSIVACRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q978P5</accession>
    <name>Q978P5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">o-succinylbenzoate synthase</fullName>
            <ecNumber evidence="5">4.2.1.113</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1418445</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.2.1.113" evidence="5"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917604.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978P5"/>
    <dbReference type="STRING" id="273116.gene:9382178"/>
    <dbReference type="PaxDb" id="273116-14325609"/>
    <dbReference type="GeneID" id="1441486"/>
    <dbReference type="KEGG" id="tvo:TVG1418445"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_4_4_2"/>
    <dbReference type="OrthoDB" id="372081at2157"/>
    <dbReference type="PhylomeDB" id="Q978P5"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="UniPathway" id="UPA01057">
        <property type="reaction ID" value="UER00165"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043748">
        <property type="term" value="F:O-succinylbenzoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016854">
        <property type="term" value="F:racemase and epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03317">
        <property type="entry name" value="NAAAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010197">
        <property type="entry name" value="OSBS/NAAAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01928">
        <property type="entry name" value="menC_lowGC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48073:SF5">
        <property type="entry name" value="O-SUCCINYLBENZOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48073">
        <property type="entry name" value="O-SUCCINYLBENZOATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00180">
        <property type="entry name" value="muconate_cycloisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00009">
        <property type="entry name" value="o-succinylbenzoate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="6">
        <location>
            <begin position="136"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="361" mass="40830" checksum="E6534E5DE666F766" modified="2001-10-01" version="1">MQIRFFKVRIPFIRPFTTSFGTEEYREAIILHMNKDGIDAFSEIVTSFLPDYGYEYNDTAINTIEKFLAKYVADAPQPHEFLDRVKGIKGHNMAKGAVEMLLWDYHSKLAGKPLDDYMGKSRGYANVGISIGMSDISEMKEQVRSAVSLGYKRIKVKIKRGRENIVSEIRDEFPDIRLSVDANGDYSYHDIPLLKELDKFELEYIEQPFRGDDLIYHSKLKKEISTPVCLDESITDPEKAEKAFEIGAVDVINIKPGRVAGLHNSIEIAKIARENNGHCWVGGMLETGIGRAFNIALASQELIDYPGDTSPNEKYFRKDIVRNPFRMKKGVIEANKGDGIGVYVDFDALFSYLVEAATISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q978X6</accession>
    <name>Q978X6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator PadR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1328580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917523.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978X6"/>
    <dbReference type="STRING" id="273116.gene:9382095"/>
    <dbReference type="PaxDb" id="273116-14325527"/>
    <dbReference type="GeneID" id="1441405"/>
    <dbReference type="KEGG" id="tvo:TVG1328580"/>
    <dbReference type="eggNOG" id="arCOG00001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_11_0_2"/>
    <dbReference type="OrthoDB" id="56053at2157"/>
    <dbReference type="PhylomeDB" id="Q978X6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF7">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="116" mass="13237" checksum="A34E27508F377030" modified="2001-10-01" version="1">MNSDPTKERILHGLITLYLLKELVRGPMHGYELQKTLSSVIGNPLPQGSIYVLLKTMKERNFVTSENAKNEKGQTLTKYYITEEGKKFLCSHSESLLIARKIIDDLLKTVDLIDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Z8</accession>
    <name>Q978Z8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Cation transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">TVG1304145</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60406.1"/>
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    <dbReference type="eggNOG" id="arCOG01576">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </evidence>
    <sequence length="678" mass="73590" checksum="C1BAC04760104E93" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Makino S."/>
                <person name="Higuchi S."/>
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                <person name="Watanabe K."/>
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                <person name="Nunoshiba T."/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="443" mass="49228" checksum="39517199C21431E0" modified="2001-10-01" version="1">MPASMVDKYRQALDLIKESKYLKIITHYDGDGTSSAIILTSMAKRMGKKFHLTYVKDLSQNGIRPLLSDDLVIFADAGSDQLRFFPDAENIIVLDHHFYQKSNYKALNINARDFGYDGTRGACGSTMAYIMALIANEENADLFPFFMSGLIADKQDLGGISGLNQVIIDAYGFKYKKERTINLEAQSMLDSITYSTDPFFEGLTGYPDKVKTFLADIEISPDATLNEINEEQKKKLANALSTLLLKQRVGLEALAYIENDIYYFDELGYNSKQLSSIVDGNGKMGQNSVPVAYFLGYQSFKDEMEKNWKMFKTKLIDYVYRAKSELFPGKNVSYFYAPESEMAGAISGILMLYLADQTRPVIGFSVGKEDTKVSSRGTRKQVNRGLNLSIVMREASQAVGGTGGGHDIAAGAVIPKGREMQFLEIADKIVGTQLGQKNETVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q979D4</accession>
    <name>Q979D4_THEVO</name>
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        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
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        <name evidence="2" type="ORF">TVG1265169</name>
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    <organism evidence="2 3">
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        <lineage>
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            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
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        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
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        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="58475" checksum="6865C491168B1701" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <property type="entry name" value="Enolase_C-like"/>
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    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
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        <property type="entry name" value="D-GALACTONATE DEHYDRATASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
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    </dbReference>
    <dbReference type="Pfam" id="PF13378">
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        <location>
            <begin position="135"/>
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        </location>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="388" mass="44122" checksum="AD2955B665B932D5" modified="2001-10-01" version="1">MYEEKITDIRTYIVRNPWKKWVFLELITNDGTVGTGEATVYNGQFSLKERIRDLGDLITGANPFEIDKFVTNWMLRTFNRSKDLISISLMSGIETALWDIMGKHFGCPVYTFLGGKFRDRIKVYANGWYTSADTIDDWKKLASGVVSRGYRALKFDPFGAGSGFLDDNEWNRARDIIESVHDAVGKNVELLIEGHGRFNRSTALKIAKYLEGFENIGWFEEPVIPEDIEGMAMIARSTSIPIAAGERYVTRFDFIKPFELGAIHIAQPDVINTGGLFEAKKIASMAEAYNIAIAPHQAEGPVNTFITMQFDATLPNLKIQEMFDEYAYPEWTWDIVDSKPEIISGYEKVEQRPGIGISLKDRVKDYLADETNKDFNLFSEGWEKRGFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q979G2</accession>
    <name>Q979G2_THEVO</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="GeneID" id="1441314"/>
    <dbReference type="KEGG" id="tvo:TVG1228987"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="108" mass="12168" checksum="0E5DE4B3C1C13FD6" modified="2001-10-01" version="1">MSWCEFQVGAGKVLVDENITSSLNGKYILLTTRVESGPCLDGMCQVIRELQAEIVDRVSENLLFFGNEKVKIAMDKSVYNSINKGREEIKLKYGRGYKLFVSGFSYVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q979H9</accession>
    <name>Q979H9_THEVO</name>
    <protein>
        <submittedName>
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    <organism evidence="2 3">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="484" mass="53703" checksum="26E183FFEA8741A5" modified="2001-10-01" version="1">MLSFERVRIRKADPVKRVGTFELEPLENYNDGEAIAEASRCIGCNMCSAACPASLDIGGYVRSTAAGDPAQTIRIIMETLPFPAIIGRVCTHMCEDVCVLYDGGGPIAIRHLKRYAADKFDDYGEILKPQKRKFIGKRVAVVGGGPAGLTVAYYLTLQGVKVTVFEERPALGGFMKTGIPRYRLPQNVLDKEIGYILSRGVQVKLNTAVGRDVKFEDLMKEYDAIFLGVGNHKPRMTGTPGSDAPNVMHATEFLERVSFGEKINVGETVAVIGGGFTANDSARTSLRLGAKHVYIMYRRRDVDRPGYPSMNADEEMEEAEEENVEYVWEVTPFEYVKENGRVVAMKYWKNEMVSQGKGRAKPVPIKDKFYTIKVDMVIEATGQETDYSFLGSYVEKLRLNDRGEPIVDQYGMTSIPGVFTGGDSTNPNRDLISAVRDGDIAVQGILRYLNVMDQVSYDDLPLLDRFKPYSYTAAAEAYASRGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q979I0</accession>
    <name>Q979I0_THEVO</name>
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        <submittedName>
            <fullName evidence="2">TVG1210382 protein</fullName>
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    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="GeneID" id="1441296"/>
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        <property type="entry name" value="DUF1634"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="126" mass="14088" checksum="A557213BC240224E" modified="2001-10-01" version="1">MKFDRDLTISYALRIGVSISMFFIIVGVILLFVKNGGSGYTLAQIASYHSQMNSKFIYLGLIPKGLLDLDGIFFIATGLWVLVFTPITSVVIALVSFYLTRNKLYIALSIIVLFNLFFAMLVIPHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q979I9</accession>
    <name>Q979I9_THEVO</name>
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        <submittedName>
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    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Six-hairpin glycosidases"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="405"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="643" mass="72809" checksum="22A81E8ECB718079" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q979J3</accession>
    <name>Q979J3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transport system protein SP1P2A</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">TVG1196198</name>
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    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917401.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979J3"/>
    <dbReference type="STRING" id="273116.gene:9381969"/>
    <dbReference type="PaxDb" id="273116-14325406"/>
    <dbReference type="GeneID" id="1441283"/>
    <dbReference type="KEGG" id="tvo:TVG1196198"/>
    <dbReference type="eggNOG" id="arCOG00151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_714992_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q979J3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050490">
        <property type="entry name" value="Bact_solute-bd_prot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649">
        <property type="entry name" value="ARABINOSE-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649:SF31">
        <property type="entry name" value="SN-GLYCEROL-3-PHOSPHATE-BINDING PERIPLASMIC PROTEIN UGPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="440" mass="48272" checksum="898D13E17424670A" modified="2001-10-01" version="1">MERKRVIAIAVVVIAVAAIAGGVVYYHNLTAAKKPTITFSGWVSSGEEYTFDVQMVNEFNSIHSNVSVKFVPITSNYYGTLETELSSNSGPAVFYMENDILPEFVKAGYLLDLTPALSANSTYNLSGFVPEILDTFMQHGQLYAAPKDWSPLLVLFNKNIFNAEHVPYPTNYTNWNWTAMKNTLELLKANESKLPNGGKGYYPMVEGPQFARILAFMHEAGGQWINSSGTGASSNSAGLLTAIKFWYGLYSSGLSGLNSNLSAGWNGGDFASGKVGMVVTGTWTVPVLFANGSYFKNDTSSIGYAFMPYDVQNATMMFNVGLAINSHLNPTQKWIAEQFVEFFTGPSGEKTWVSKGLALPSRSAILDSSWYKTNFPIQSFAGEQFPFAYGWNYNTTNFQATETAAHNIIVDLFAGKISPTQAYYQILNETNTNLKGTSTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q979K3</accession>
    <name>RL18E_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L18e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18e</name>
        <name type="ordered locus">TV1158</name>
        <name type="ORF">TVG1187063</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60300" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60300.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917392.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979K3"/>
    <dbReference type="SMR" id="Q979K3"/>
    <dbReference type="STRING" id="273116.gene:9381958"/>
    <dbReference type="PaxDb" id="273116-14325396"/>
    <dbReference type="GeneID" id="1441274"/>
    <dbReference type="KEGG" id="tvo:TVG1187063"/>
    <dbReference type="eggNOG" id="arCOG00780">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146465_0_0_2"/>
    <dbReference type="OrthoDB" id="11309at2157"/>
    <dbReference type="PhylomeDB" id="Q979K3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00329">
        <property type="entry name" value="Ribosomal_eL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021132">
        <property type="entry name" value="Ribosomal_eL18/eL18-A/B/_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022947">
        <property type="entry name" value="Ribosomal_eL18_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003079">
        <property type="entry name" value="PRK04005.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01106">
        <property type="entry name" value="RIBOSOMAL_L18E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000132806" description="Large ribosomal subunit protein eL18">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="122" mass="13409" checksum="8921E44F8B7847D8" modified="2002-05-27" version="2">MSLTVTTKENEALVNEIRSLVEISRQNSAKIWRDMAERLANGRRRYASINLYKIDKFAKDGDVIVVPGSVLGVGKITKKVTIGAVHFSRAATEKLVRSGCAFMSLSDVAKANPKGTNVKIMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q979N2</accession>
    <name>Q979N2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase I chain L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1157047</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917362.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979N2"/>
    <dbReference type="STRING" id="273116.gene:9381927"/>
    <dbReference type="PaxDb" id="273116-14325366"/>
    <dbReference type="DNASU" id="1441244"/>
    <dbReference type="GeneID" id="1441244"/>
    <dbReference type="KEGG" id="tvo:TVG1157047"/>
    <dbReference type="eggNOG" id="arCOG01540">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_1_5_2"/>
    <dbReference type="OrthoDB" id="29144at2157"/>
    <dbReference type="PhylomeDB" id="Q979N2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00445">
        <property type="entry name" value="NDH1_NuoN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010096">
        <property type="entry name" value="NADH-Q_OxRdtase_suN/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004445">
        <property type="entry name" value="PRK05777.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22773">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="442"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="6">
        <location>
            <begin position="113"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="473" mass="51836" checksum="E6D014036689C641" modified="2001-10-01" version="1">MSLISIFIPEILLVIMGFIVLAVGMFIQSGKASAAISFITLIAALLSVFFIPYDSSYGITVNKFSVYFSAVLIISALFISLPMYRYVKKRPEVFYASLIFSVIGMVIVAMTWNLVIAFVAFESVSIMTYIMSAYGGKQRNLEATIKYFFTGTIATTFIILGIGFYFFSVHTFSLSPVLNGSSISYYSGTKPEFLLALLFLLIGFGFKLAIFPMHQWAIDAYDGTENGVSAYLSTGTKIMAFVLVLKIFLVGFQSFPTPVYYMFTIIAIITMTYANVAALSQMNVKRLLAYSSVAQAGYMILVISVVSYVGLGSARSVSLAIAAGMFYALVYVFMKGGSFIAMNMVKEDNITFENISGLGKKSPYVAASFSILLLSLAGIPLTGGFLAKYFLFLSVIDSGLWWLAIIAIINSAISVFYYMKVMRYLFWEEPKGEFSTDMGNKAAVIVSAAIVVFLGVFYVLFPVLVGISSQIVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q979N7</accession>
    <name>TDXH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peroxiredoxin</fullName>
            <ecNumber evidence="1">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Thioredoxin-dependent peroxiredoxin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV1123</name>
        <name type="ORF">TVG1154375</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodecamer. Pentamer of dimers that assemble into a ring structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this 1-Cys peroxiredoxin, no C(R) is present and C(P) instead forms a disulfide with a cysteine from another protein or with a small thiol molecule.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917357.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979N7"/>
    <dbReference type="SMR" id="Q979N7"/>
    <dbReference type="STRING" id="273116.gene:9381922"/>
    <dbReference type="PaxDb" id="273116-14325361"/>
    <dbReference type="GeneID" id="1441239"/>
    <dbReference type="KEGG" id="tvo:TVG1154375"/>
    <dbReference type="eggNOG" id="arCOG00312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_4_2"/>
    <dbReference type="OrthoDB" id="6924at2157"/>
    <dbReference type="PhylomeDB" id="Q979N7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00401">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022915">
        <property type="entry name" value="Peroxiredoxin_TDXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009668">
        <property type="entry name" value="PRK13189.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009669">
        <property type="entry name" value="PRK13190.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF128">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0049">Antioxidant</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0575">Peroxidase</keyword>
    <keyword id="KW-0676">Redox-active center</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135174" description="Peroxiredoxin">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="3"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="1">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00401"/>
        </source>
    </evidence>
    <sequence length="203" mass="23252" checksum="9CE5C7536380BD9A" modified="2001-10-01" version="1">MPVVLGQKAPDFTVNTSKGPITLSNYRGKWVLLFSHPGDFTPVCTTEFIAFTERYEDFQKLGVELIGLSIDSVFSHIAWIRDIKEHFGIDIPFPIIADIDKEVAREYNLIDEKSGATVRGVFIIDPNQIVRWMIYYPAETGRNIEEIIRVIKALQFNWDRKLATPANWQPGQEGIEPAPTTVDSSFKRDNEQGVKTWYLKFVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q979V6</accession>
    <name>TRPF_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName>PRAI</shortName>
            <ecNumber>5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trpF</name>
        <name type="ordered locus">TV1054</name>
        <name type="ORF">TVG1080245</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917283.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979V6"/>
    <dbReference type="SMR" id="Q979V6"/>
    <dbReference type="STRING" id="273116.gene:9381848"/>
    <dbReference type="PaxDb" id="273116-14325292"/>
    <dbReference type="GeneID" id="1441165"/>
    <dbReference type="KEGG" id="tvo:TVG1080245"/>
    <dbReference type="eggNOG" id="arCOG01983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076364_2_0_2"/>
    <dbReference type="OrthoDB" id="27513at2157"/>
    <dbReference type="PhylomeDB" id="Q979V6"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002302">
        <property type="entry name" value="PRK01222.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894:SF1">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154416" description="N-(5'-phosphoribosyl)anthranilate isomerase">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="195" mass="21326" checksum="FE3C91205EE080D3" modified="2001-10-01" version="1">MKIKVCGITRLEDAAMVTELGASIVGVVLDELSPRHASHNTIREIAEAGITVAGVYTSEQTVLSSPLFEDYVQLHFDHDPELIRSIHELGRKVISVINFNGIRDLKIKYNAYREADIILVEYKKGVSSIVSQIAPLGLNVGYAGGISDRDIENIIAAKPSIIDVSSSLESSPGIKHPGKVMSFFKKIRDAMDYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q979V7</accession>
    <name>Q979V7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">anthranilate synthase</fullName>
            <ecNumber evidence="3">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1079200</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917282.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979V7"/>
    <dbReference type="STRING" id="273116.gene:9381847"/>
    <dbReference type="PaxDb" id="273116-14325291"/>
    <dbReference type="GeneID" id="1441164"/>
    <dbReference type="KEGG" id="tvo:TVG1079200"/>
    <dbReference type="eggNOG" id="arCOG02014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_7_1_2"/>
    <dbReference type="PhylomeDB" id="Q979V7"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005009">
        <property type="entry name" value="PRK06404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="7">
        <location>
            <begin position="108"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="351" mass="39611" checksum="515EA5C4D40533AB" modified="2001-10-01" version="1">MLADQIDEFRTYSNFAYFASFPDRVNGYEYVFASDTFQNDPSKIFDGALRPILVTYDYVNEIFDTKVKSSGWPYFLSMDAETIEKRPIMRTSAFKKKNSGSLKDEALEGKIRKIVNLIRNGEVLQVVISREFEANVNVNEKIHEFLTKDRSRYVFYYRFGKYEVVGSSPENIFTVIGNRIYVDPIAGTISHSFSSDTLYRSEKDRCEHRMLLDLARNDVSKFADIGSLEVPKVMEIEEFSSVKHLVSQVKATFSNKNYVDILRAMFPAGTVTGSPKERAIEIIDKFEETPRGPYGGAVGIVGSGIFDMGLAIRMAYSSGNGFRVRAGAGIVKDSDPSSEVNEIYNKARSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q979X4</accession>
    <name>Q979X4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cystathionine beta lyase / O-succinylhomoserine lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1061416</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979X4"/>
    <dbReference type="STRING" id="273116.gene:9381829"/>
    <dbReference type="PaxDb" id="273116-14325274"/>
    <dbReference type="KEGG" id="tvo:TVG1061416"/>
    <dbReference type="eggNOG" id="arCOG00060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q979X4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000046">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF50">
        <property type="entry name" value="CYSTATHIONINE BETA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="368" mass="40814" checksum="0A44BE9A061921D3" modified="2001-10-01" version="1">MPIKDRFDDHAHSLSFPVFFTSSYDSRKDRKYRYSRELNPTVEELSRQLSLLENTERSICFSSGMGAITGTILALAKPGDKLVMPTDVFGRTYLFAKNFLTKFGINVKFVEPGTDSLLGAVEEHADIVFFESLSNPLLRLYDVDRICRKASDIGAITIVDNTLATPILLKPSLYGCDIIIESLSKFIGGHNNAIGGSASGSLMMDTIDENRRSLGSSMDPMSAYLFILGLKTIDLRIKRSSETAKAIARFLNENNFVSKVYYPGLSDNSDNISAKTLLNGSPPIVSFLLDEGKDVQKFMESLRIITPANTMGGSNSTISNPYTMSHRNLSEDEKLRLGITKNLMRLSVGLEDLDQIIQDLEQAIKYAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q979X9</accession>
    <name>Q979X9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thiamine monophosphate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1054535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979X9"/>
    <dbReference type="STRING" id="273116.gene:9381824"/>
    <dbReference type="PaxDb" id="273116-14325269"/>
    <dbReference type="DNASU" id="1441142"/>
    <dbReference type="KEGG" id="tvo:TVG1054535"/>
    <dbReference type="eggNOG" id="arCOG00638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046964_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q979X9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270:SF3">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PurM-like C-terminal" evidence="1">
        <location>
            <begin position="84"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="221" mass="24943" checksum="CFB1970AAA7F14AE" modified="2001-10-01" version="1">MKVLKMYDVDFIGGDLKEGMDLILTGIAIGKQKKTLTRKRSDIGRKQMVAYTNKLGRAASGYIFYKSGYDSVEGINMMLGITPRLEEARIISEYGAKFMMDLSDGLYSSLNQMKADYGVGFKIVEDEVVYDDSARKAAEISGASVYDIACGYGGDYELLFTVPEENYRDFAQAMESNNIEVHFIGETWEGENIVFDGKTWTKMKDEGYEHFRPKPQLGEIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Y3</accession>
    <name>PDXS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="1">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="1">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxS</name>
        <name type="ordered locus">TV1027</name>
        <name type="ORF">TVG1050816</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917256.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Y3"/>
    <dbReference type="SMR" id="Q979Y3"/>
    <dbReference type="STRING" id="273116.gene:9381820"/>
    <dbReference type="PaxDb" id="273116-14325265"/>
    <dbReference type="GeneID" id="1441138"/>
    <dbReference type="KEGG" id="tvo:TVG1050816"/>
    <dbReference type="eggNOG" id="arCOG04075">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055352_1_0_2"/>
    <dbReference type="OrthoDB" id="6840at2157"/>
    <dbReference type="PhylomeDB" id="Q979Y3"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033983">
        <property type="entry name" value="Thiazole_synthase_ThiG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00343">
        <property type="entry name" value="pyridoxal 5'-phosphate synthase lyase subunit PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05690">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000109451" description="Pyridoxal 5'-phosphate synthase subunit PdxS">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="1">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="278"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <sequence length="336" mass="36655" checksum="8E538C75EBA89892" modified="2001-10-01" version="1">MALDLEKLKFGTELIKRGFSSMTKGGVIMDVTTAEQAKIAEKAGAVAVMALERVPADIRAAGGVARMADPKKIREILDAVTIPVMAKVRIGHISEAYTLEKLGVDMLDESEVLTPADPFFHLYKKKLTVPVVSGARNLPEAVRRIFEGAAMVRTKGEAGTGNIIEAVRHIRKVNEEIAIVRRMTQDEIKTAAENIASSYFQLRNEASTDLFGQSGKVLYDDVYYGMSREKIVKGISDILRQIRKLNRLPVVNFAAGGVATPADGALMMELGADGVFVGSGIFKSPDPEKMAKSIVEAVENYKDYDVVARVSEGLQGMPGIDIESIPRDSRLQERGW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-31" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Y4</accession>
    <name>SYN_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Asparagine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Asparaginyl-tRNA synthetase</fullName>
            <shortName evidence="1">AsnRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">asnS</name>
        <name type="ordered locus">TV1026</name>
        <name type="ORF">TVG1050051</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917254.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Y4"/>
    <dbReference type="SMR" id="Q979Y4"/>
    <dbReference type="STRING" id="273116.gene:9381819"/>
    <dbReference type="PaxDb" id="273116-14325264"/>
    <dbReference type="GeneID" id="1441136"/>
    <dbReference type="KEGG" id="tvo:TVG1050051"/>
    <dbReference type="eggNOG" id="arCOG00407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_0_2"/>
    <dbReference type="OrthoDB" id="5908at2157"/>
    <dbReference type="PhylomeDB" id="Q979Y4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04323">
        <property type="entry name" value="AsnRS_cyto_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00534">
        <property type="entry name" value="Asn_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003037">
        <property type="entry name" value="PRK03932.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000176490" description="Asparagine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00534"/>
        </source>
    </evidence>
    <sequence length="429" mass="49893" checksum="92ACE28F75CEDE87" modified="2001-10-01" version="1">MLSIAEASSEAHVGSEVSVRGWVYRIRSSGGVTFIVIRDSTGIVQCTAKKNELTPEQYDEISSLGIESSLSLIGTMKKEPRSPTGYEISIHKFTVYQKNDVFPITKDQGEEFLLDNRHLWLRSREFTSILKVRSTIFRSFADFFYDQGYYQVHTPFMVSTAVEGGSTLFKVDFFGEPIFLNQSAQFYLETMIYSLEKVFTIAPSFRAEKSRTRRHLTEYWHAEAEVAWIDNREMMDVEENMIYYIIQRVIEENYDDLKLLGRDPEALRAMKPPFPRVKYSDIIKVANEIGMQLKYGDDLGADEERQITMRYCKPVFVTNYPKELKPFYMPQDPDNPSEVLNHDLLAPEGYGEIIGGSQRIWDYKELMQRIREAGLDESAYYWYIDLRKYGSVPHSGFGLGMDRLAMWIMHLDNIREAIPYPRTIRRTKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Z8</accession>
    <name>DPHB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diphthine synthase</fullName>
            <ecNumber evidence="1">2.1.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dphB</name>
        <name type="ordered locus">TV1012</name>
        <name type="ORF">TVG1034064</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36415"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="2.1.1.98"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.98" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917240.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Z8"/>
    <dbReference type="SMR" id="Q979Z8"/>
    <dbReference type="STRING" id="273116.gene:9381805"/>
    <dbReference type="PaxDb" id="273116-14325250"/>
    <dbReference type="GeneID" id="1441122"/>
    <dbReference type="KEGG" id="tvo:TVG1034064"/>
    <dbReference type="eggNOG" id="arCOG04161">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066040_0_1_2"/>
    <dbReference type="OrthoDB" id="39139at2157"/>
    <dbReference type="PhylomeDB" id="Q979Z8"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004164">
        <property type="term" value="F:diphthine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156131" description="Diphthine synthase">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="111"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01084"/>
        </source>
    </evidence>
    <sequence length="206" mass="22828" checksum="E6D9E77FC2D21D7B" modified="2001-10-01" version="1">MLNIIGIGLRGTGSLTLDEFDALRTSDIVYLDIYTSIGPKDILEKLRNIADREIIPADRNMIESESILKDAEKLNVSLLVIGDGLTATTHNQLRYSAMEKGIKVKIFENASAVNTAAGKIGLLHYKVGPPVSLPFVSSNFFPLSVIDKVKRNYDSGLHTPILIDLKDGQNMPFASAWNIIMEMQKRKGVCNNRRKCMRCLEAFISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A03</accession>
    <name>Q97A03_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1029778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917235.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A03"/>
    <dbReference type="SMR" id="Q97A03"/>
    <dbReference type="STRING" id="273116.gene:9381800"/>
    <dbReference type="PaxDb" id="273116-14325245"/>
    <dbReference type="DNASU" id="1441117"/>
    <dbReference type="GeneID" id="1441117"/>
    <dbReference type="KEGG" id="tvo:TVG1029778"/>
    <dbReference type="eggNOG" id="arCOG04212">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114051_2_3_2"/>
    <dbReference type="OrthoDB" id="179876at2157"/>
    <dbReference type="PhylomeDB" id="Q97A03"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34988:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34988">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="138" mass="15646" checksum="3C7DDFEBD3F98702" modified="2001-10-01" version="1">MYSKIEGSIITARFDKETDFFTDLVDLCNKYEVKSGTVLWAIGMLKEFQIGYWNGNDYEKETFRERLELVSLHGSIAENDPRFHIHAAGARGNHGIVGGHLFSAKVDPLVEIQIMKITGFSMSRELDNSDGLYKLVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A33</accession>
    <name>Q97A33_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Archaeal Type IV pilin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0999061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A33"/>
    <dbReference type="STRING" id="273116.gene:9381769"/>
    <dbReference type="PaxDb" id="273116-14325194"/>
    <dbReference type="KEGG" id="tvo:TVG0999061"/>
    <dbReference type="eggNOG" id="arCOG02423">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431718_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012859">
        <property type="entry name" value="Pilin_N_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07790">
        <property type="entry name" value="Pilin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Type IV pilin N-terminal" evidence="2">
        <location>
            <begin position="35"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="189" mass="20014" checksum="4BF06D8DB20C29A1" modified="2001-10-01" version="1">MTKDAQVWRNAVSSELILILICMYQCIMKLNRQDEAVSPIIATILLIAITIVLAATLYSLLGGYVSLISSPTPTASITITNETSSTSPDSIYSIYVSSVNANVSLTDVKLEIISLDGNVTEAALSPGTIYISDWAITVHGPDYLSALTVITITSQPGNPSPYIKYVKLVDIVTDGTIAGQSLGPASQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A38</accession>
    <name>Q97A38_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alcohol dehydrogenase [ADH]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0995648</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917202.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A38"/>
    <dbReference type="STRING" id="273116.gene:9381764"/>
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    <dbReference type="KEGG" id="tvo:TVG0995648"/>
    <dbReference type="eggNOG" id="arCOG01455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="8709at2157"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:small molecule metabolic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Zn_ADH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
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        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010344">
        <property type="entry name" value="PRK13771.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="8"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="335" mass="35933" checksum="0162350CEEF36A1D" modified="2001-10-01" version="1">MKAAILKKINEPVSIDDVDIPDPAENEVLIKQEYAGVCYRDLLAATGFFPRIALPMIQGHEIGGTVVKVGDKVEAFRPGDKVSSLIYIPCGKCEFCRSGNENLCPYKKSLGEEVQGGFRQYVNIPEISLVKAPNNVDGASLSLAACVTGMLYHALKRMGKAGEGKKVLITGAGGGVGIHAVEMAKAFGATVIAETTSPEKLDLLEKIGADYIVDGNSKFNEDVKKLGGADIVLECVGIYTFERSLRSLNNGGKMVIIGNVKPDPVSLPLGLIILKGNTIRGSISSTKKDVEEALNMVSKGMIKPIVGKEIDISQINEAYKLMKDKKSSGRIMIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A69</accession>
    <name>Q97A69_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enoyl-CoA hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0968612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A69"/>
    <dbReference type="STRING" id="273116.gene:9381733"/>
    <dbReference type="PaxDb" id="273116-14325158"/>
    <dbReference type="KEGG" id="tvo:TVG0968612"/>
    <dbReference type="eggNOG" id="arCOG00242">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_3_2"/>
    <dbReference type="PhylomeDB" id="Q97A69"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="249" mass="28134" checksum="F0D6C4DC7665EA1C" modified="2001-10-01" version="1">MHKMPRCVLEEVDDIAILKFTRSEKLNAFDFDSWREFSSNVDEASNYKGLIITGEGRAFSSGDDINAMYKFEDYAESKSFFKTLFDVLQKLMHYPHPIIAAVNGLAAGGGAEILLTLDYVVSVKDAWFWFPEARIGLYPPILTSLGVYVFGIKNVKKLAINMPRLTSEEALNLGLVDMIIEDKNKLTEIAVNKVRELNRVPTVSYTSMRKVLASIVLPHLKQSLDTLAETAVEEEAKRSMELFIKSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A70</accession>
    <name>Q97A70_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0967781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A70"/>
    <dbReference type="STRING" id="273116.gene:9381732"/>
    <dbReference type="PaxDb" id="273116-14325157"/>
    <dbReference type="KEGG" id="tvo:TVG0967781"/>
    <dbReference type="HOGENOM" id="CLU_2140343_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="112" mass="13234" checksum="742F9C11FCFE3DFF" modified="2001-10-01" version="1">MNICRGQASLAYIKDIFAYCPTNEKSSQHHANALTKTCRCKIRLCKSIRHNENNEIVKLRAFFSNEKAMHNNFGRCVKYRRLRLICVACGIMLRLGLVMYWRLMLFKYKTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A88</accession>
    <name>Q97A88_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">dTDP-glucose 4,6-dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0950610</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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            <begin position="4"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAB60064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="318" mass="36252" checksum="8421B8476140E895" modified="2001-10-01" version="1">MKLLVTGGAGFIGSNFINYWLKKHQRDSIVNVDKLTYAANPDYVDHKSFSDRYELIKADIANAKQIESIIKDVDCVVNFAAESHVDNSIKSPEPFIRSNYVGVYNILEAVRKYDIRFHQISTDEVFGSLPLDSSQKFDEHSPYAPRNPYSATKAAADMLVRSYINTYGIKATISNCSNNYGPNQHREKLIPKTVYNAIHNFRIPIYGSGRQIRDWIHVLDHCSAIEAILERGRIGETYLVSARNEQHNIDVVKKILGILGKDESLIEYVSDRPGHDVRYAIDPKKIENELDWKPSIPFDEGLRDTVNHYASLFSNELR</sequence>
</entry>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
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            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="340" mass="40314" checksum="089EE66FFE92B367" modified="2001-10-01" version="1">MKYISVIITAYNRKEFLLDAIKSALNQTLDRKYYEIIVIKNFQEDTIDNFIKENNIKGIISENKSLGGKISEALKASEGEIVSFLEDDDMFDRQKLETVYRKFRENPDIVYYHNNQIIIDENGKEIRKNGVLNERKLNSTEVRKSPLDINPMLFNMSSISIRRSYYAKYADNLSSLITHLDDFMLFCALNESTYIYVDKAFLTYYRYHASASNMGIRINDKIDTQQYALLKANILKGYCEASFFMLQIFENKFLKELINFRYNMECLFLNFYSCKSYAKSEVKRSMLIYILKSKKIKPNIFNLRRRKWVKLYTVFIYLYLTIFNLCNTPFKTVMIKLLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A99</accession>
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        </submittedName>
    </protein>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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    <dbReference type="GeneID" id="24779952"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="68" mass="8276" checksum="6BB22A356B3CF2ED" modified="2001-10-01" version="1">MCREIENSEGSISTFLTQRKERSYRKGYRKIKLNAVKEKPDKTNIRYVRFSKQMDSGYIEEKRKLWII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AA0</accession>
    <name>Q97AA0_THEVO</name>
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    </protein>
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    </gene>
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        <lineage>
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            <taxon>Thermoplasmatota</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="tvo:TVG0934632"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="56" mass="6763" checksum="044EEECCC6727ED0" modified="2001-10-01" version="1">MRNVMKRIPKKKQHLIIQIIKRVLEDETCTNNSIYFLKKVLHYSYLPFIKSTKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AB1</accession>
    <name>Q97AB1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0920010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AB1"/>
    <dbReference type="STRING" id="273116.gene:9381691"/>
    <dbReference type="PaxDb" id="273116-14325116"/>
    <dbReference type="KEGG" id="tvo:TVG0920010"/>
    <dbReference type="eggNOG" id="arCOG07825">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237980_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25753" checksum="7350775D1415562C" modified="2001-10-01" version="1">MNMDRNGNSQETGYEQTHAERHKLMLRDIIKLAGSDKLVCNIGLSSFDKLAVNYLGDRYYCAVPNVEFLKSVDQLYISEQNIIYYDITKNNPEPNSKFDLVIISETLEHIFADDEIVMRSIANLLRPNGIIYLTVPNAARHINRIKLLFGKNIFWSKYDILNGGYGHIREYTVIEVKSMLEKYFDVRQIYGFNPYGSKLIRISLNLLPTSWRSTIVAIGMKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AD1</accession>
    <name>Q97AD1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0898023 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0898023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917124.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AD1"/>
    <dbReference type="SMR" id="Q97AD1"/>
    <dbReference type="PaxDb" id="273116-14325096"/>
    <dbReference type="DNASU" id="1441972"/>
    <dbReference type="GeneID" id="1441972"/>
    <dbReference type="KEGG" id="tvo:TVG0898023"/>
    <dbReference type="eggNOG" id="arCOG07738">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032985_0_0_2"/>
    <dbReference type="OrthoDB" id="378744at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="121" mass="14577" checksum="0CDB1EC0B8DEA6AE" modified="2001-10-01" version="1">MDMRSLFHDIHRTVMNAMERAGYSGKATLKILRTPRSWYYVQLDFSPLLDGRFNSFAVREDDEWIVIGYRRKQPEMSFREIAYTLIDEDLTYLSPQSVYRILKKHDLITEWHMKTWPSTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AF0</accession>
    <name>Q97AF0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-oxoglutarate synthase subunit KorA</fullName>
            <ecNumber evidence="2">1.2.7.11</ecNumber>
            <ecNumber evidence="8">1.2.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-ketoglutarate oxidoreductase alpha chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">2-oxoglutarate-ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">TVG0881751</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + 2-oxoglutarate + CoA = succinyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17297"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="1.2.7.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotetramer of the KorA, KorB, KorC and KorD subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="2"/>
    <dbReference type="EC" id="1.2.7.3" evidence="8"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917104.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AF0"/>
    <dbReference type="STRING" id="273116.gene:9381652"/>
    <dbReference type="PaxDb" id="273116-14325077"/>
    <dbReference type="DNASU" id="1441952"/>
    <dbReference type="GeneID" id="1441952"/>
    <dbReference type="KEGG" id="tvo:TVG0881751"/>
    <dbReference type="eggNOG" id="arCOG01606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_1_0_2"/>
    <dbReference type="OrthoDB" id="31112at2157"/>
    <dbReference type="PhylomeDB" id="Q97AF0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000022">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000009">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022367">
        <property type="entry name" value="2-oxoacid/accept_OxRdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053400">
        <property type="entry name" value="2-oxoacid_Fdx_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03710">
        <property type="entry name" value="OAFO_sf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041170">
        <property type="entry name" value="Oxoac_fdxalpha_Archa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF16">
        <property type="entry name" value="PYRUVATE FLAVODOXIN_FERREDOXIN OXIDOREDUCTASE DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="12">
        <location>
            <begin position="12"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="13">
        <location>
            <begin position="243"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="14">
        <location>
            <begin position="514"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAB60002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="629" mass="69214" checksum="33EAF01938717D9B" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AI3</accession>
    <name>Q97AI3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0838588</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917071.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AI3"/>
    <dbReference type="STRING" id="273116.gene:9381617"/>
    <dbReference type="PaxDb" id="273116-14325043"/>
    <dbReference type="GeneID" id="1441919"/>
    <dbReference type="KEGG" id="tvo:TVG0838588"/>
    <dbReference type="eggNOG" id="arCOG02041">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051844_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AI3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051406">
        <property type="entry name" value="PLD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856">
        <property type="entry name" value="CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856:SF1">
        <property type="entry name" value="MITOCHONDRIAL CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="4">
        <location>
            <begin position="230"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="290" mass="33567" checksum="4F38DD5358851906" modified="2001-10-01" version="1">MTIYVEPQDGIVPIIKFIRRVRKKIFMNFYLIDDDRIINEIREAKKRGVDVRIIVDGKPYGMGQSKVDVKELSSTCASVKLSPPRFDASDVFDHAKYMVSEKYFEIGTANMTEASFSRNREYIYIGKNRKIRKCLMSLFISDWENSNETGIRCKELVISPGSESTILHILRTSKLVESEEVGDDTRIIETLSSRRRKLKIVIPSSASQNDIANMKKLRERGVKARLMPPDRLYMHAKAVLFKDYVFIGSQNFTSTSLNRNREVGIIIRSLSDRRKIKRIMVADWKRSEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AL3</accession>
    <name>Q97AL3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycerate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0797109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917041.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AL3"/>
    <dbReference type="STRING" id="273116.gene:9381587"/>
    <dbReference type="PaxDb" id="273116-14325013"/>
    <dbReference type="GeneID" id="1441889"/>
    <dbReference type="KEGG" id="tvo:TVG0797109"/>
    <dbReference type="eggNOG" id="arCOG04170">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032279_1_1_2"/>
    <dbReference type="OrthoDB" id="10741at2157"/>
    <dbReference type="PhylomeDB" id="Q97AL3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10180">
        <property type="entry name" value="Glycerate kinase, MOFRL-like N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1480.10">
        <property type="entry name" value="MOFRL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037035">
        <property type="entry name" value="GK-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038614">
        <property type="entry name" value="GK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054996">
        <property type="entry name" value="Gly_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007835">
        <property type="entry name" value="MOFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025286">
        <property type="entry name" value="MOFRL_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039760">
        <property type="entry name" value="MOFRL_protein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040791">
        <property type="entry name" value="gly_kin_Thpl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227:SF0">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13660">
        <property type="entry name" value="DUF4147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05161">
        <property type="entry name" value="MOFRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82544">
        <property type="entry name" value="GckA/TtuD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MOFRL-associated" evidence="2">
        <location>
            <begin position="29"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="MOFRL" evidence="1">
        <location>
            <begin position="311"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="420" mass="46266" checksum="EAC7D918A8FF022B" modified="2001-10-01" version="1">MPFSVKNISDVAYDERRAFIFRTLNEVFKDFEPNKVTEKAVNEIDLNKYAGVYVIGFGKAAYEMYLGVREHVKRKLKYAGIIIPSDQEVQYLPELDILRGTHPLTSTLSVTSSKKLLSKARPSANDLVLVLISGGGSSLFEIPQEGITIDQMASISRAMMDHSANIYELNTIRSALSSVKGGKLARILYPATIIALIISDVPGDDISIIASGPLAENKLDPSAVYAKYRDIIPIDITKYVENSSIEDLYFRNVMNRIILSNRDFVFEIYKRINEPIVSFGSNIQGDVTEVSEAFVRSIYEISKIKGKSFWFVAGGETTVNVKGNGIGGRNLELALRFMKLANFSDFLFLSIGTDGIDGVSPAAGGIVSSDMKLKISSQELEETLDRNDAFTLLSAYHGAIMTGRTGNNVSDIMVGYVSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AM1</accession>
    <name>Q97AM1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Small heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hsp14</name>
        <name evidence="4" type="ORF">TVG0791352</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
            <strain evidence="4">GSS1</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2010-12" db="EMBL/GenBank/DDBJ databases">
            <title>Improving protein stability and enzyme recovery under stress conditions by using a small HSP (Tpv HSP 14) from Thermoplasma volcanium.</title>
            <authorList>
                <person name="Semra K."/>
                <person name="Sema A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Type strain: 4299</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2010-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Kocabiyik S."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Type strain: 4299</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="FR745403">
        <property type="protein sequence ID" value="CBY78065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917033.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AM1"/>
    <dbReference type="SMR" id="Q97AM1"/>
    <dbReference type="STRING" id="273116.gene:9381579"/>
    <dbReference type="PaxDb" id="273116-14325005"/>
    <dbReference type="GeneID" id="1441881"/>
    <dbReference type="KEGG" id="tvo:TVG0791352"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_9_5_2"/>
    <dbReference type="OrthoDB" id="198277at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041799">
        <property type="entry name" value="Hsp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="22"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CBY78065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="124" mass="14334" checksum="F7A3CE63E5E7016B" modified="2001-10-01" version="1">MYTPIKFFTNEMIKNVSNTVKEVSSFIYPPVTLYQDSSDLVLEAEMAGFDKKNIKVSVNKNVLTISAERKREYSTVYIDQRVDKVYKVVKLPVEIEQQDISAKYSEGILTVRMKTKNIKNVEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AQ1</accession>
    <name>Q97AQ1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">succinate dehydrogenase</fullName>
            <ecNumber evidence="4">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">TVG0761794</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917005.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AQ1"/>
    <dbReference type="STRING" id="273116.gene:9381548"/>
    <dbReference type="PaxDb" id="273116-14324974"/>
    <dbReference type="GeneID" id="1441853"/>
    <dbReference type="KEGG" id="tvo:TVG0761794"/>
    <dbReference type="eggNOG" id="arCOG00571">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_2_2"/>
    <dbReference type="OrthoDB" id="23539at2157"/>
    <dbReference type="PhylomeDB" id="Q97AQ1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000003">
        <property type="entry name" value="Fumarate reductase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004724">
        <property type="entry name" value="PRK06069.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="12">
        <location>
            <begin position="7"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="13">
        <location>
            <begin position="442"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB59900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="567" mass="63208" checksum="628F2A220BF5E2D1" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AS5</accession>
    <name>Q97AS5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="1">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0739837</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="1441831"/>
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    <dbReference type="eggNOG" id="arCOG02635">
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        <location>
            <begin position="8"/>
            <end position="97"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047942"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="102" mass="11783" checksum="18EC5199B0FF2CA6" modified="2001-10-01" version="1">MIDGPSISDESFKWKAEFPEIMKNGGFYIVIGNPPYVDIKGLDPTIVKYLFSNYTTVENRMNPYSAFVERAANLLKNYGYFGFIIPNSILYNESYKKHGLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="GeneID" id="24779925"/>
    <dbReference type="KEGG" id="tvo:TVG0735005"/>
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    <dbReference type="Proteomes" id="UP000001017">
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="323" mass="38638" checksum="6B951DCF2D857A62" modified="2001-10-01" version="1">MSENQYITKKEIIEELLYKDRNFFLNNNPEFNIFKYTAFFAVVTFTFQPIIVMVNIFSAFSYLIYIQLLEFQLSFYFIIALIMLYIGLTRAIRTSYYNRKGVYLRLYKCRICDFTSFSEFYSNLHIVSHPDHTLKYNQILIKYAKEPKKTWFTRLSTISKRKRLEKNYDRDFIILSKTDQRNENEIKGGKWLQVRSVGIILSIIILQAYIYYSYSLFRSPEIIIGTLIIFGIFYIICFYIFIQLNTQDVENAHIFKLELEYKQEQENPYIVFHHAKIDLSYWKSINKKLKLKPIEVQDGELFIVDKIVINNCGEIIDAIPEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AU1</accession>
    <name>Q97AU1_THEVO</name>
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        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
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        <name evidence="7" type="ORF">TVG0724374</name>
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    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AU1"/>
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    <dbReference type="PaxDb" id="273116-14324934"/>
    <dbReference type="GeneID" id="1441824"/>
    <dbReference type="KEGG" id="tvo:TVG0724374"/>
    <dbReference type="eggNOG" id="arCOG03650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_20_3_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="PhylomeDB" id="Q97AU1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
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        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
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    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="22"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAB59860.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="476" mass="50893" checksum="5AC54118D4A615B4" modified="2001-10-01" version="1">MNDTSAGHLKSNAVGFWPLLGQAIAMISPLGAVAATMTGSASIALGSLPLAYLIAIFAVLFWINTPYQYSKKIASAGGFYTYNRNGAGQYYGSISGYVLFFSYYMTYTNAILFITAVFIPGLFEIFFGITLTAWIWIPVLIAIGLLVLLPAYLGIEGSTKYSLASSIIQIALLVILSLTIIVIKGPANTLEPFTPKPAGGFGPVFVGMILAIFSMSGSSAVVSLGEEAKQPKRNIRNALLISFLVTGVVFVLTSYALTIGWGISNMNTFAQSNPAAGLTVGQGVPGIIEALKYMGMPMAIAMMIFAVNSLYTGSLAPLNSAARMFFAMSRDGIAPKVFAKTHGKYKTPHVALVFIAFTGILVSLIAGLAMGPFNGFLYLVTASAVALFIGHIMTDISLPVAFKKFREFSVLKHAVLPAISLVLLLIGIYYSFFPPTYPTNIAIITAVIFMVAVAIGINVYLRNGQRKMPTSYESDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AU9</accession>
    <name>Q97AU9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TVG0719175 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG0719175</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916968.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="STRING" id="273116.gene:9381499"/>
    <dbReference type="PaxDb" id="273116-14324926"/>
    <dbReference type="GeneID" id="1441816"/>
    <dbReference type="KEGG" id="tvo:TVG0719175"/>
    <dbReference type="eggNOG" id="arCOG03669">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_255700_0_0_2"/>
    <dbReference type="OrthoDB" id="56693at2157"/>
    <dbReference type="PhylomeDB" id="Q97AU9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1346"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="10">
        <location>
            <begin position="294"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="502"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="1376" mass="147896" checksum="430384BC49873FDD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AV7</accession>
    <name>Q97AV7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0708255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053968.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AV7"/>
    <dbReference type="STRING" id="273116.gene:9381491"/>
    <dbReference type="PaxDb" id="273116-14324918"/>
    <dbReference type="GeneID" id="1441809"/>
    <dbReference type="KEGG" id="tvo:TVG0708255"/>
    <dbReference type="eggNOG" id="arCOG05302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034545_0_2_2"/>
    <dbReference type="PhylomeDB" id="Q97AV7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13672">
        <property type="entry name" value="PP2C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="220" mass="24549" checksum="A89E2FA3D51AFF50" modified="2001-10-01" version="1">MEMIKVEYFSDSSAVPHLHLENEDSYSVTDDLFIVADGVGSYEGSKDASRYAVNYLSKMAKEIQSKEQLVEEIIKLSEEIKSIGIISGRPLMSTTISVLKISTEKYITANVGDSPIVLLRSGKLYKLYIDDSERSSTGNRFALEQAFGWNHVIVHSFEGKLEKGDLFIICTDGVSDNLSDEYDLYSILRELDAQTIVRLALNKGIKPDDATIIKVYVTMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AW1</accession>
    <name>Q97AW1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0703424 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0703424</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916956.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AW1"/>
    <dbReference type="STRING" id="273116.gene:9381486"/>
    <dbReference type="PaxDb" id="273116-14324914"/>
    <dbReference type="GeneID" id="1441804"/>
    <dbReference type="KEGG" id="tvo:TVG0703424"/>
    <dbReference type="HOGENOM" id="CLU_1850745_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="138" mass="15830" checksum="4F3EA7B506429BDF" modified="2001-10-01" version="1">MIEIKDAEETYLRVLDHFFSGWIVVSGDHNAIIKLEQIVYMPGTAKNEGLELIMIPPRTLVLAKKTPELRKALKLDNDTKLWDSLVWLILTPEGDTVLADSRHFPADMPEVNRDMFFEELAKELIYGKEGGRIDETME</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AW8</accession>
    <name>Q97AW8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Insertion element</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0698004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916950.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AW8"/>
    <dbReference type="STRING" id="273116.gene:9381479"/>
    <dbReference type="PaxDb" id="273116-14324907"/>
    <dbReference type="GeneID" id="71201315"/>
    <dbReference type="KEGG" id="tvo:TVG0698004"/>
    <dbReference type="eggNOG" id="arCOG10339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2217199_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AW8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13683">
        <property type="entry name" value="rve_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="27"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="106" mass="12056" checksum="A8243AAF1BCEAF9B" modified="2001-10-01" version="1">MHSQGLCKASGRCNAIGYTNFNLNNLVLRTDNSPQYIAKGFKHTARLLNIPHEYIVKQTPDDNGDIESFHNSLKTDYIRLNDLESYEDAKELIEYASNEYNPVSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AW9</accession>
    <name>Q97AW9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0697170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AW9"/>
    <dbReference type="STRING" id="273116.gene:9381478"/>
    <dbReference type="PaxDb" id="273116-14324906"/>
    <dbReference type="KEGG" id="tvo:TVG0697170"/>
    <dbReference type="eggNOG" id="arCOG11984">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_36_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="TrpR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="67" mass="7574" checksum="39C07A29404E57BC" modified="2001-10-01" version="1">MMEKFTADEKAAIAMESFTAINIAGLCRRHGVSVSNFYKWRDKFIESGKQGFYGSGVNNGYEKENEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AZ1</accession>
    <name>Q97AZ1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dihydrodipicolinate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0663048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916927.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AZ1"/>
    <dbReference type="STRING" id="273116.gene:9381456"/>
    <dbReference type="PaxDb" id="273116-14324884"/>
    <dbReference type="GeneID" id="1441775"/>
    <dbReference type="KEGG" id="tvo:TVG0663048"/>
    <dbReference type="eggNOG" id="arCOG04172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_5_1_2"/>
    <dbReference type="OrthoDB" id="350860at2157"/>
    <dbReference type="PhylomeDB" id="Q97AZ1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00953">
        <property type="entry name" value="KDG_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="2">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="288" mass="32148" checksum="E297114E6CA89DE5" modified="2001-10-01" version="1">MKLKIVPVVTPFKEGKIDGEKFIHHCMSLLSVGVNYVFISGTTGLGPSLSINERMDLIKLSSNFADKAIMQVGSMNLEDSIKLARMAKENGIYGISALPPYYFGGIPEEWLIKYYVEISKIIPTFIYNYPKFTGYDINSSLAIKINKAGGNIIGVKDTIADISHMLSYRDLGNDFIILSGPSNSIYEALRSGVDGSVAAAGNFMPKTFIELADKFEAEASFKLQKYINSVLSIANEYGSWSAYYSLIKILRRYSAGDSRAPFYPLNALDEEKLRHDVFFVLPEEQRHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B00</accession>
    <name>Q97B00_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0653073 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0653073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916918.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B00"/>
    <dbReference type="STRING" id="273116.gene:9381447"/>
    <dbReference type="PaxDb" id="273116-14324875"/>
    <dbReference type="GeneID" id="1441766"/>
    <dbReference type="KEGG" id="tvo:TVG0653073"/>
    <dbReference type="eggNOG" id="arCOG00328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363719_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="1">
        <location>
            <begin position="15"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="200" mass="23002" checksum="23D3342EE150D6DA" modified="2001-10-01" version="1">MQSQLTDNLRAFVKQIKKSELISFDIETLVEDGKFLNNENIIAISYCTGDLNCNVFVAESEGEEETILQNFDLLLGNVKPAIILGYNHTGYDIPLISYKIRNFKDYKFWNIREYFGSAYTLDVMYLVKNLTGKIMKLEDAISLYLGKSELEAKMIPSLNGMGKGEAIKYLWKNERKKFETYSLNDSISTLKIFYKIMCDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-25" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B26</accession>
    <name>HEM3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable porphobilinogen deaminase</fullName>
            <shortName evidence="1">PBG</shortName>
            <ecNumber evidence="1">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="1">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemC</name>
        <name type="ordered locus">TV0633</name>
        <name type="ORF">TVG0625731</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="1">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916892.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B26"/>
    <dbReference type="SMR" id="Q97B26"/>
    <dbReference type="STRING" id="273116.gene:9381421"/>
    <dbReference type="PaxDb" id="273116-14324849"/>
    <dbReference type="GeneID" id="1441740"/>
    <dbReference type="KEGG" id="tvo:TVG0625731"/>
    <dbReference type="eggNOG" id="arCOG04299">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_2"/>
    <dbReference type="OrthoDB" id="8042at2157"/>
    <dbReference type="PhylomeDB" id="Q97B26"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000005">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000143033" description="Probable porphobilinogen deaminase">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="1">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <sequence length="297" mass="32892" checksum="01067904DF95820D" modified="2001-10-01" version="1">MKIKIGTRPSNLAVAQANMVASSLEAIGIDTEIVKHRSAGDIDTKNPIYSIGKTGVFVQDLNNMILRGEIDVAVHSAKDIPSEIENRLTIAATLKRGSFNDALVSKYDLRSLPISAKVGTSSIRRIFQLKYARQDLKFENIRGNIETRIAKMSELGLDAIVMAEAAIQRLGLNVEYSKLDENKFVPAPNQGIIAVVSKKEGTVRKILEEINDENTFEEMQIERSVVQQLKLGCSTPVGILSRPSGKGHKIIAQFFSMQGDDVSVFEQYLNDLSDVDSLVSYIRENIPREYGYIASEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B64</accession>
    <name>Q97B64_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0582526</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916852.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B64"/>
    <dbReference type="STRING" id="273116.gene:9381382"/>
    <dbReference type="PaxDb" id="273116-14324809"/>
    <dbReference type="GeneID" id="1441700"/>
    <dbReference type="KEGG" id="tvo:TVG0582526"/>
    <dbReference type="eggNOG" id="arCOG02690">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_1_2"/>
    <dbReference type="OrthoDB" id="56388at2157"/>
    <dbReference type="PhylomeDB" id="Q97B64"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="3"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="403" mass="44236" checksum="42141CF268D6CA4E" modified="2001-10-01" version="1">MHTIIATYLGWSLDAFDFFLITFVMLAIAKTFNVALIVVTLSITLTLSMRFIGSLFFGPIGDKYGRKITMIISLLFLAFFDTLVAFSPTITIFLILRALYGFGMGGEWGSGFALAMESLPAKARGWVSGLIQGGYPTGYLLAAVVYLAIYPRFGWRPMFFFAALPAIAVIYIMLTTRESPVYAEYKERASFKKTLTTIGKNWKLFVYLSILLIGMNFVSHSTQDLYPTFLEHQLKFSPTYAASIAIAYNAAAIVGGILSGIISQNTRLGRKWNAVAFLAVGIVISPLWAFPQLFGHSSLIYMAFLAPVVMQFIVQGAWAMPGVYMNERSPAEARATIPGVAYMVGVMVTSTAASIEVAVSSMFSDNYSYSLLLLVLIVFPLTMVAWALGKESKNARLDVSSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B86</accession>
    <name>Q97B86_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0558589</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916830.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B86"/>
    <dbReference type="STRING" id="273116.gene:9381357"/>
    <dbReference type="PaxDb" id="273116-14324786"/>
    <dbReference type="GeneID" id="1441088"/>
    <dbReference type="KEGG" id="tvo:TVG0558589"/>
    <dbReference type="eggNOG" id="arCOG04356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038336_0_0_2"/>
    <dbReference type="OrthoDB" id="57367at2157"/>
    <dbReference type="PhylomeDB" id="Q97B86"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008508">
        <property type="entry name" value="Bax1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39640:SF1">
        <property type="entry name" value="DUF790 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39640">
        <property type="entry name" value="VNG6129C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05626">
        <property type="entry name" value="DUF790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019435">
        <property type="entry name" value="UCP019435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="410" mass="46703" checksum="2B486E5C6790DEBF" modified="2001-10-01" version="1">MFPLELLVAKKTENGSIRPIVIPPDSKWPAENVIDTFRSSIGLKRRELYERIKDIEYQAKNPKTVRSMALLLERISKFRDSFQVPSSEVRRFLFSLGPAVRPEERHTLVEMAAKHFSVGKQEIEDALYGDIEPEQRLISVPDITPDWLVRNYNLEQIETLIYKAKSMVVKKLSNWLPFIDAIKELGLMFEALNEEGLTVIIDGPNSIFGGMDRYGSALAQAFSLLAGSSSWSISATVSIGGKDYSVQLDQSLNYYLPEKREGMLDKTYPEPINIDGRLYFPSSVMDIDGKKVYVDIVRNDDINRILKRDSKIRASGYNWITVYIGPSKNWSNYPLRFRSKIDWSLVQAYARKTLPKETKADYVMAALSKLYPDIDAIIDFLDSNGLPLSIVEKYGYKVSWNGIVPSIEKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BC6</accession>
    <name>PGK_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="1">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pgk</name>
        <name type="ordered locus">TV0530</name>
        <name type="ORF">TVG0521991</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916788.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BC6"/>
    <dbReference type="SMR" id="Q97BC6"/>
    <dbReference type="STRING" id="273116.gene:9381314"/>
    <dbReference type="PaxDb" id="273116-14324745"/>
    <dbReference type="GeneID" id="1441046"/>
    <dbReference type="KEGG" id="tvo:TVG0521991"/>
    <dbReference type="eggNOG" id="arCOG00496">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_2"/>
    <dbReference type="OrthoDB" id="6575at2157"/>
    <dbReference type="PhylomeDB" id="Q97BC6"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000006">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000012">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF23">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000146074" description="Phosphoglycerate kinase">
        <location>
            <begin position="1"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="60"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="358"/>
            <end position="361"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <sequence length="408" mass="44891" checksum="403FB7056E6F5849" modified="2001-10-01" version="1">MADFFLMDSFDLAGRTIYLRVDINSPVNPVTGEIMGTDRFRAHVETIRKLRDSKVVIVAHQSRPGKDDFTSLRQHAQVMSRILNKKVMFVDQLFGSLVNKTVESMNEGDIVMLENARFYSEEVDLTTLESMENSNIVKGLSTLFDYYIIDAFAAIHRAQTTLVGFRRIKPNIAGALIEKEVTMIDRFRHLNESPKIAILGGAKIDDSIAVSENFLKSGFVDKILTGGVVANAFLWAKGIDIGKKNRDFIIKNNGDYEKLIAKCKGLLSEFGDRILVPSDFILSPSGERVSANGKIPDDQILADIGLDTVVEYSEIIDKAKAIFMNGPMGIYEIEAYSSGTREIFSSVAKSEAFSIAGGGHTLSALDKLGLTNRIDHASTGGGALISYLSGEAMPVLEALKESKRLFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BD0</accession>
    <name>Q97BD0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0517148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916784.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BD0"/>
    <dbReference type="STRING" id="273116.gene:9381310"/>
    <dbReference type="PaxDb" id="273116-14324741"/>
    <dbReference type="GeneID" id="1441042"/>
    <dbReference type="KEGG" id="tvo:TVG0517148"/>
    <dbReference type="eggNOG" id="arCOG01469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096331_0_0_2"/>
    <dbReference type="OrthoDB" id="97972at2157"/>
    <dbReference type="PhylomeDB" id="Q97BD0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF3">
        <property type="entry name" value="ABC-TYPE MULTIDRUG TRANSPORT SYSTEM, PERMEASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="241" mass="26526" checksum="AE79C2945FF97466" modified="2001-10-01" version="1">MNLRYISLFAWYYGFRAVKRGPSYLISAMSTPLALLFLIFIISRGKLLDYALVGGFIGLVSSVSLSSAGDAAFLRLQLRIQDLFVATSISPTDYMFGLTLSYFVFSIPGLVVYVILGIIFHVFTFIGVLVLVAVLILLTISTSSISFIVSGLISHVRNVWGIAGILSVVMTVLPPTFYPYYIIPKAFLYALSVSPAASAAVIVQGAFGLSPILMQMLYIFILETIIYAFLGRVMIKWHSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BE3</accession>
    <name>Q97BE3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0501372</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916771.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BE3"/>
    <dbReference type="STRING" id="273116.gene:9381296"/>
    <dbReference type="PaxDb" id="273116-14324728"/>
    <dbReference type="GeneID" id="1441029"/>
    <dbReference type="KEGG" id="tvo:TVG0501372"/>
    <dbReference type="eggNOG" id="arCOG00020">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020520_0_0_2"/>
    <dbReference type="OrthoDB" id="43786at2157"/>
    <dbReference type="PhylomeDB" id="Q97BE3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000003">
        <property type="entry name" value="Phosphomethylpyrimidine kinase ThiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="266" mass="28550" checksum="AAE6ACDBC5688C05" modified="2001-10-01" version="1">MPIPKALTIAGSDSGGGAGIQADLKTFNSLSVFGMSVITAVTAQNSYEVSGILPVPPEFVKLQIDTVAKDIKVDGAKTGMLFSSEIIKVVTGSIKDYSIKPVIVDPVMVSKSNARLLREDAIESLIKYLLPESTIVTPNIPEAEILASMSISTIEDMKAAAEKIGTEIGLKVLVKGGHFNGPPIDIYYDGSNILKFEGTRYETKNTHGTGDTLSSAILSFLIKGYREQEAIALGKEYVEGSIRNSFPTGSGFGSLCHWWRYGECHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BF2</accession>
    <name>Q97BF2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Galactokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0491501</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the GHMP kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916762.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BF2"/>
    <dbReference type="STRING" id="273116.gene:9381287"/>
    <dbReference type="PaxDb" id="273116-14324719"/>
    <dbReference type="GeneID" id="1441020"/>
    <dbReference type="KEGG" id="tvo:TVG0491501"/>
    <dbReference type="eggNOG" id="arCOG01030">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048558_1_0_2"/>
    <dbReference type="OrthoDB" id="116110at2157"/>
    <dbReference type="PhylomeDB" id="Q97BF2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050201">
        <property type="term" value="F:fucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042352">
        <property type="term" value="P:GDP-L-fucose salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052203">
        <property type="entry name" value="GHMP_Kinase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001174">
        <property type="entry name" value="HddA/FKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014606">
        <property type="entry name" value="Heptose_7-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32463">
        <property type="entry name" value="L-FUCOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32463:SF0">
        <property type="entry name" value="L-FUCOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036406">
        <property type="entry name" value="Hept_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00960">
        <property type="entry name" value="LMBPPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="6">
        <location>
            <begin position="76"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="7">
        <location>
            <begin position="230"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="329" mass="36719" checksum="AB31645039D2D61C" modified="2001-10-01" version="1">MILSYSPLRITFGGGGTDIEPFVSKYGGAVVNATIDRGVTVRYIDDGYQTELSSRDFVKSYIINMHGPSTVSSRMLDYLLRSGLRTGRIIMSGDVPPGSGLGSSSAAMSALVNLTSIIRKTKYNWESIARESYNIEKNYFHIVLGLQDPYAIALGGFKFMEFNGDGVKYEMLDKYGDFTSELEKRIILIYTGHTRQSSEVLIDQVRAATQGDQETTEKLLQLKEVAFRLRKAVIDNDYSEFDQAINYGWEIKKTLGQKTTNRRVDTIIESALKNGASAARLMGGGSQGFILVLSRPGKINELQRAMMNASNFVVRTSFDKRGTRRLFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BH1</accession>
    <name>Q97BH1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription elongation factor Spt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spt4</name>
        <name evidence="4" type="ORF">TVG0469462</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of Spt4 and Spt5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal Spt4 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916743.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BH1"/>
    <dbReference type="STRING" id="273116.gene:9381266"/>
    <dbReference type="PaxDb" id="273116-14324699"/>
    <dbReference type="GeneID" id="1441001"/>
    <dbReference type="KEGG" id="tvo:TVG0469462"/>
    <dbReference type="eggNOG" id="arCOG04077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199467_0_0_2"/>
    <dbReference type="OrthoDB" id="275101at2157"/>
    <dbReference type="PhylomeDB" id="Q97BH1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00949">
        <property type="entry name" value="Spt4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007178">
        <property type="entry name" value="Spt4_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038589">
        <property type="entry name" value="Spt4_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005003">
        <property type="entry name" value="PRK06393.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041664">
        <property type="entry name" value="RNAP_arch_Epp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="3">
        <location>
            <begin position="5"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="64" mass="7374" checksum="9A7D170E9A19903C" modified="2001-10-01" version="1">MKVQYRACKKCKRLTPEKTCPIHPDEKTTTEWFGFLIITEPEFSAIAKRAGISEPGMYAIKVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BH7</accession>
    <name>Q97BH7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF998 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0466379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BH7"/>
    <dbReference type="STRING" id="273116.gene:9381260"/>
    <dbReference type="PaxDb" id="273116-14324693"/>
    <dbReference type="KEGG" id="tvo:TVG0466379"/>
    <dbReference type="eggNOG" id="arCOG02008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100105_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BH7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42241">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF998 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42241:SF2">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF998 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22658" checksum="252BB1F7C5F071FF" modified="2001-10-01" version="1">MMQRDFYNLIFFTGIAAIICAWLTIAISIHFNPWFRINVNALSDLGGGNFISNGHPAPTYPFIYNGGLMVTALLVFSFSVLSISHVNSKVEIMGLSFFIISALFLALIGIYHEGTYPHAFVSIWFFILSSISFVTIGIGMYLVGDKKFGITIILIILASWIVYSSVRWGSVAEGEVFGISVIDIVVSIYLANLAIVRAKKVRLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BJ7</accession>
    <name>Q97BJ7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0449104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053930.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BJ7"/>
    <dbReference type="STRING" id="273116.gene:9381239"/>
    <dbReference type="PaxDb" id="273116-14324673"/>
    <dbReference type="GeneID" id="1440975"/>
    <dbReference type="KEGG" id="tvo:TVG0449104"/>
    <dbReference type="eggNOG" id="arCOG05397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_277820_0_0_2"/>
    <dbReference type="OrthoDB" id="57496at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1077"/>
            <end position="1099"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="1171" mass="132419" checksum="D347291D8573E6BE" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BK2</accession>
    <name>Q97BK2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N5-carboxyaminoimidazole ribonucleotide synthase</fullName>
            <shortName evidence="4">N5-CAIR synthase</shortName>
            <ecNumber evidence="4">6.3.4.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">5-(carboxyamino)imidazole ribonucleotide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">purK</name>
        <name evidence="7" type="ORF">TVG0442698</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + hydrogencarbonate + ATP = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PurK/PurT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.18" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BK2"/>
    <dbReference type="STRING" id="273116.gene:9381234"/>
    <dbReference type="PaxDb" id="273116-14324668"/>
    <dbReference type="KEGG" id="tvo:TVG0442698"/>
    <dbReference type="eggNOG" id="arCOG01597">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BK2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034028">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="99"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="139"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="164"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="243"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="359" mass="40650" checksum="839DA0A4F2731108" modified="2001-10-01" version="1">MMNVGIIGSGQLGYMMINEGRRLGNKYFVLDSNGVGPASLIADAHFSVDQYKEFVDKCDVVTYEFEHVLEEALLYAEDQHKLKPSIRPVELKKDRSLEKDYLTGIGLKVANYRVAHSFSEAVRYAKDFGKAMIKNSFGGYDGKGQTIYDNGNYEELKGEKFVVEEFVDFDYEASIIASRSEDKSFRYHDPSFNYNRNAIFIYNYAPIMPANMPDIAKKLMDSLDYVGVMGIEFYVKNGEVIINEFAPRVHNSGHHTLLGSSISQFEEHVRAITGIPISQPILYRPSSSINILGTKLSTDAWNSILSMSSTQIYWYGKEEIRRRRKVGHVNVTAGTVEALIDKIGKIMEIIYPDGIENYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="139" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BL5</accession>
    <name>Q97BL5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0430262</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916698.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BL5"/>
    <dbReference type="STRING" id="273116.gene:9381219"/>
    <dbReference type="PaxDb" id="273116-14324655"/>
    <dbReference type="DNASU" id="1440956"/>
    <dbReference type="GeneID" id="1440956"/>
    <dbReference type="KEGG" id="tvo:TVG0430262"/>
    <dbReference type="eggNOG" id="arCOG04116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023387_0_0_2"/>
    <dbReference type="OrthoDB" id="7146at2157"/>
    <dbReference type="PhylomeDB" id="Q97BL5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09878">
        <property type="entry name" value="PIN_VapC_VirB11L-ATPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052041">
        <property type="entry name" value="Nucleic_acid_metab_PIN/TRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010335">
        <property type="entry name" value="PRK13764.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603">
        <property type="entry name" value="AAA FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603:SF147">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PIN" evidence="5">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="254"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="477"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00046345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="66227" checksum="31C17E5F351757C0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BM5</accession>
    <name>Q97BM5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RfbU related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0420283</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916688.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BM5"/>
    <dbReference type="STRING" id="273116.gene:9381209"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324645"/>
    <dbReference type="DNASU" id="1440946"/>
    <dbReference type="GeneID" id="1440946"/>
    <dbReference type="KEGG" id="tvo:TVG0420283"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_3_2"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="PhylomeDB" id="Q97BM5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="83"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="194"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="369" mass="42087" checksum="972F82188EF54A21" modified="2001-10-01" version="1">MKIAVIGWELPPAFSGGLGIHTVNMFSIIGYYRNVDLYVPDLCYSFPKYPFNVRKVKIEKGIDRSAYSRITDFYEAVMDYNEKVVDAFDPNGVKLVHCHDWITFPAGIAIKEKYGIPLIVTYHSTEFDRSAYFNPQEKIMKIEREGGKEADRIITVSNLTKSIVVEKYNIDPEKIVTVYNGVDATHYLSFPDVKKERNVLYFGRVTNQKGPKFFMETAKKVLEFDRTIRFTIAGTGELLGEMKSYAMDNDFFDHIEFPGFVHFRKAIWYYKRSSAFIIPAVSEPFGMTVLEAMVSGTPVILSRTTGVGEALHHVLSADFWDTDRMATYVVSILNHRGILETMSRYGKAEALGFTWDKAAAKTMEVYDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BM9</accession>
    <name>Q97BM9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0411609 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0411609</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_156769048.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BM9"/>
    <dbReference type="PaxDb" id="273116-14324641"/>
    <dbReference type="GeneID" id="1440941"/>
    <dbReference type="KEGG" id="tvo:TVG0411609"/>
    <dbReference type="eggNOG" id="arCOG07369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800821_0_0_2"/>
    <dbReference type="OrthoDB" id="56363at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="346" mass="40076" checksum="137524378DFE1226" modified="2001-10-01" version="1">MNSILMPPSLDYLVAVRVKVPNPIPEKYWKSISDEFYGIQKYYHIGAMFVKESGHWNILMNAFKVLPTEEVISIVDYINNVYTKMGLGTVVSNDAKHIIRSITDISDKSIDRLAGIDGLVLYPVISIGNNELIMVFEYPEEASDEVSRMILDYVNKAPLPAEIIKMEMKKPGDKPSLFKAIELKGIDPSSVLILHTRWFMKLEEFDRQVGGIFRNLMAFRIKKFDRDAFQLIAEVFGNELKFDGQYKVYRKSEKSQIVQFTLNTGWFKDFFDDVIKVFMGPVDYWGYSDGIGNVDNYFMIRKSDQADFLKALGKHWKNPIRYYHRNRLMEIVPMSDILPSDPRNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BN8</accession>
    <name>Q97BN8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG0404408 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0404408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916674.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BN8"/>
    <dbReference type="STRING" id="273116.gene:9381195"/>
    <dbReference type="PaxDb" id="273116-14324632"/>
    <dbReference type="GeneID" id="1440932"/>
    <dbReference type="KEGG" id="tvo:TVG0404408"/>
    <dbReference type="eggNOG" id="arCOG05351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165227_0_0_2"/>
    <dbReference type="OrthoDB" id="55978at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.290">
        <property type="entry name" value="Hypothetical membrane protein ta0354_69_121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036171">
        <property type="entry name" value="Ta0354_soluble_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024504">
        <property type="entry name" value="Unchr_Ta0354_soluble_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11433">
        <property type="entry name" value="DUF3198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116858">
        <property type="entry name" value="Hypothetical membrane protein Ta0354, soluble domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Uncharacterised membrane protein Ta0354 soluble" evidence="2">
        <location>
            <begin position="72"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="121" mass="14085" checksum="2DBF49B88BC868F0" modified="2001-10-01" version="1">MEKSIRDFQFIIYAALFVISMAVLVIVSNQLIFHNSFLNDAQFNVGDWVYWIFAVSFIAAIMFGYSMWANLRDTSRFESLINSSSKSVFVRNLDELERLAKKLGKGYLIQLENAKEKWKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BN9</accession>
    <name>Q97BN9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1805 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0403403</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BN9"/>
    <dbReference type="STRING" id="273116.gene:9381194"/>
    <dbReference type="PaxDb" id="273116-14324631"/>
    <dbReference type="KEGG" id="tvo:TVG0403403"/>
    <dbReference type="eggNOG" id="arCOG04424">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160472_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BN9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1980.10">
        <property type="entry name" value="Hypothetical protein YunC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014931">
        <property type="entry name" value="DUF1805"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036493">
        <property type="entry name" value="YunC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08827">
        <property type="entry name" value="DUF1805"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102891">
        <property type="entry name" value="Hypothetical protein Ta1206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12592" checksum="C0709D36BCBBAD5A" modified="2001-10-01" version="1">MKIELSTNRVIYAFQRYHVTMIEIDTVNLNGKAFQFFRMGLGNAPLLILKGEHGYAMCGYLNLDAAEKLGDTAVRITGIKTLDDMLNGKIVEVTSAASALGIKKGDVLKDVLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BP6</accession>
    <name>Q97BP6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">4-hydroxybenzoate octaprenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0396861</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BP6"/>
    <dbReference type="STRING" id="273116.gene:9381187"/>
    <dbReference type="PaxDb" id="273116-14324624"/>
    <dbReference type="KEGG" id="tvo:TVG0396861"/>
    <dbReference type="eggNOG" id="arCOG00477">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034879_5_1_2"/>
    <dbReference type="PhylomeDB" id="Q97BP6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006371">
        <property type="entry name" value="Polyprenyltransferase_UbiA-li"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009532">
        <property type="entry name" value="PRK12895.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01475">
        <property type="entry name" value="ubiA_other"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="290" mass="33113" checksum="5CE60CE575E774AA" modified="2001-10-01" version="1">MKKSMKFKDIVDYIKLEHTVFDLPFILSGYVIAAGTSIFPYKIILILIAAVTARSSAMSINRIEGLRYDLTNPRKKEWALVSGRMTVREAIALTILFIAVFEISAFLLNRLVFILSPIVIFLFVSDPILKRYTEWRHFYMGSAIGIGTLAGYLAVIPEFPSNLAIYFLFIASSLWIAGFDMIYVIPDIEYDQKNKLKTVMTKYGIKLGLLISDITHLAALIFFWLMLYYFRTIWYLAGLIIITILVIYQHIIVDPADPLSIRKSFFNANSFIGFIFLITVILSVASPLWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BT2</accession>
    <name>Q97BT2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MMGE/PRPD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0362515</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpD family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916627.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BT2"/>
    <dbReference type="STRING" id="273116.gene:9381150"/>
    <dbReference type="PaxDb" id="273116-14324588"/>
    <dbReference type="GeneID" id="1440885"/>
    <dbReference type="KEGG" id="tvo:TVG0362515"/>
    <dbReference type="eggNOG" id="arCOG04285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026574_3_0_2"/>
    <dbReference type="OrthoDB" id="43639at2157"/>
    <dbReference type="PhylomeDB" id="Q97BT2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4100.10">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.120">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042183">
        <property type="entry name" value="MmgE/PrpD_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042188">
        <property type="entry name" value="MmgE/PrpD_sf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045336">
        <property type="entry name" value="MmgE_PrpD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943:SF8">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03972">
        <property type="entry name" value="MmgE_PrpD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="247"/>
        </location>
    </feature>
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        <location>
            <begin position="259"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="443" mass="50006" checksum="069EF4F8A7CD1880" modified="2001-10-01" version="1">MDKTVEVLEDYANSTSFSDINDDEKEAIKMRIADSFITAFTAISSPPTKILLNTIGDVRGQMDSTIYFSKRKVFTPYAVMLNGCMTRYMDYNDTYLSKEALHPSDNIPPLITMAETKELEGKDVIRAVKVAYDIVCSFADAVSIRDRGWDHVNYDSISSSAALASMLELNGEKFENAVSLSIINNISMRQTRAGKLSMWKGCTVAYETMSSTLAVLNASAGLTGPSDIFEGEMGFKKQVSGEFQLEIKPHVMKTMIKNYPVEYHAMSAAQAAANLKDKLDGNIKKIDVETFKVAHTIIIKDPEKLRPQNKETADHSMPYIIAYTLNYGEPDVHSYDDKYLNDKKILDTIDKIHFKVTERFDKMYPEYLPVKISVTDQTSVKEEEIDVPKGHFRDPYTWKDLEAKARRVNPNLVELVSYVRNIENKSLVELKEVMTNVAAEGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
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    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="273116.gene:9381143"/>
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    <dbReference type="eggNOG" id="arCOG03697">
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FERREDOXIN-TYPE PROTEIN NAPH"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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    </feature>
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        </location>
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        </location>
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        <location>
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    </feature>
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="649" mass="73709" checksum="89904DF0FB6C4109" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BU8</accession>
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        <recommendedName>
            <fullName evidence="1">Nop domain-containing protein</fullName>
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        <name evidence="2" type="ORF">TVG0348814</name>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="264" mass="30362" checksum="ADD24BCEEE98DF1C" modified="2001-10-01" version="1">MWRQNMEKAMWYGIGKRMYKDLDSRYIEIFEMLRMGRTPELDGEGKKPDLRSLLIEYGNYRIKQEFTEDQAAIKVAAVREEMDQLINKYYEKCLAFAIPFDLKVTSDPCAYFRQGSTIDGISELFSYGSKMCDFRGQLDERVKEMARQILPNTTALVGERLAMDLLVHAHGLKRLAYMPSSSIQMLGAERALFTAIQKRGNTPKYGVLFKYPKLSTLRPRQRGKIARIIANKVAITARADLLGTKVDAESFREKIDNMIKSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BV3</accession>
    <name>Q97BV3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Protein translocase subunit SecY</fullName>
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            <fullName evidence="10">Protein transport protein SEC61 subunit alpha homolog</fullName>
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        <lineage>
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    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 13">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BV3"/>
    <dbReference type="STRING" id="273116.gene:9381129"/>
    <dbReference type="PaxDb" id="273116-14324567"/>
    <dbReference type="KEGG" id="tvo:TVG0345339"/>
    <dbReference type="eggNOG" id="arCOG04169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031763_3_0_2"/>
    <dbReference type="PhylomeDB" id="Q97BV3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00303">
        <property type="entry name" value="SECYTRNLCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="335"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="14">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAB59494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="565" mass="61704" checksum="651357581C80884A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BV9</accession>
    <name>RL32_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL32</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L32e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl32e</name>
        <name type="ordered locus">TV0346</name>
        <name type="ORF">TVG0341099</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916600.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BV9"/>
    <dbReference type="SMR" id="Q97BV9"/>
    <dbReference type="STRING" id="273116.gene:9381123"/>
    <dbReference type="PaxDb" id="273116-14324561"/>
    <dbReference type="GeneID" id="1440858"/>
    <dbReference type="KEGG" id="tvo:TVG0341099"/>
    <dbReference type="eggNOG" id="arCOG00781">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071479_3_1_2"/>
    <dbReference type="OrthoDB" id="372100at2157"/>
    <dbReference type="PhylomeDB" id="Q97BV9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00810">
        <property type="entry name" value="Ribosomal_eL32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023654">
        <property type="entry name" value="Ribosomal_eL32_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006332">
        <property type="entry name" value="PRK08562.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000131168" description="Large ribosomal subunit protein eL32">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="128" mass="15168" checksum="3C8DED83F9A37456" modified="2001-10-01" version="1">MNRALDDEVRRLLKIKNYMARKRVEFHRQEWFRYKKLGDAWRKPRGKHSKLREHLSRRPPIVDAGFRSPAKVRGMHPSGYYEVLVYNLKDIENIDPKIQAARIASSVGSRKREEIAKRCAELNIKVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BZ2</accession>
    <name>Q97BZ2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA pseudouridine synthase Pus10</fullName>
            <ecNumber evidence="5">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridine 54/55 synthase</fullName>
            <shortName evidence="5">Psi54/55 synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pus10</name>
        <name evidence="8" type="ORF">TVG0322286</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Responsible for synthesis of pseudouridine from uracil-54 and uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine(54) in tRNA = pseudouridine(54) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:57876"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="5"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916569.1">
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    <dbReference type="KEGG" id="tvo:TVG0322286"/>
    <dbReference type="eggNOG" id="arCOG01015">
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:tRNA pseudouridine synthesis"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="HAMAP" id="MF_01893">
        <property type="entry name" value="Pus10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005912">
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    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048741">
        <property type="entry name" value="Pus10-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055174">
        <property type="entry name" value="Pus10_THUMP_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21238">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22023">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pus10 THUMP" evidence="7">
        <location>
            <begin position="75"/>
            <end position="147"/>
        </location>
    </feature>
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        <location>
            <begin position="161"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
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            <position position="213"/>
        </location>
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    <feature type="binding site" evidence="5">
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            <position position="278"/>
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        <ligand>
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        <location>
            <position position="350"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="388" mass="44313" checksum="0C99CAADFE15A982" modified="2001-10-01" version="1">MVSLSDLASYPICRRCAGRIFAMVGTGLTNLERGSMLEFSIKCEKNDPEFNFADPQNCEICHGIFDKFSVFLSLVEDKLKEIEYSTFVVGSVFPKEWIELENKIQEAFGAHGESIKKEFNREFGKYITAKTGKEYRKEDPDITIAVNVEYEFVNVSIKSLFIYGKYRKYRRDMPQTRWIHKKDGDSIESIIGEAALRIAGGKNYYLHGSGREDVDVRMLGNGREFVIEVEAPIMRTFDLSILSETVNRSMKGVEIFDLSFVDRKTVKEIKEEKHSKVYDCIIEGDTDIDKLKLEEACTEMNGKNIYQRTPLRVAQRRSDLIRTRKIERMELVAVEGKEAEIVVQAEAGTYIKELISGDSGRTNPSLSSKYGSQLNVKELDVIKICRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C43</accession>
    <name>Q97C43_THEVO</name>
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        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0274704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C43"/>
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    <dbReference type="PaxDb" id="273116-14324476"/>
    <dbReference type="GeneID" id="1440777"/>
    <dbReference type="KEGG" id="tvo:TVG0274704"/>
    <dbReference type="eggNOG" id="arCOG01078">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140804_0_0_2"/>
    <dbReference type="OrthoDB" id="25379at2157"/>
    <dbReference type="PhylomeDB" id="Q97C43"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="109" mass="12556" checksum="D5161E94EEEEE430" modified="2001-10-01" version="1">MVSTRTGGKIQAVIFRCKEKPEVLILHYRPEHGSFWQTVSGNVELNEDLLDSLIREVKEETGLGKECIINVYPDIYSFDFHAHDMDFHETVFAVMVRPDCNVDISRNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C52</accession>
    <name>Q97C52_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HPP transmembrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0266639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048053918.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C52"/>
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    <dbReference type="PaxDb" id="273116-14324467"/>
    <dbReference type="GeneID" id="1440769"/>
    <dbReference type="KEGG" id="tvo:TVG0266639"/>
    <dbReference type="eggNOG" id="arCOG07744">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129119_0_0_2"/>
    <dbReference type="OrthoDB" id="57378at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058581">
        <property type="entry name" value="TM_HPP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04982">
        <property type="entry name" value="TM_HPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="HPP transmembrane region" evidence="2">
        <location>
            <begin position="4"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="150" mass="17047" checksum="ADDA76AE90C6E317" modified="2001-10-01" version="1">MNRWKNILSFLAIFGIIIVWTYVFRLALLAPPFAVSAYLISVEHRGRFSKPDSLIVSYVAVILSTTLLHILLGTGVIQLYLNVVIISILITFTRFKHPPAIALAVFSYIEHNDVKFVESSIIILIVLWLADLIHHHYVFLADESEDKEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C84</accession>
    <name>Q97C84_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0227715 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0227715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_052268456.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C84"/>
    <dbReference type="STRING" id="273116.gene:9380990"/>
    <dbReference type="PaxDb" id="273116-14324433"/>
    <dbReference type="GeneID" id="71201307"/>
    <dbReference type="KEGG" id="tvo:TVG0227715"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2379589_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="94" mass="10024" checksum="A68674ACF5527044" modified="2001-10-01" version="1">MNGATKNVSFENSITNSNLIENEERKFRRKLGLLDLFMIGITGVIGSGWLFSPLYAANAAGPASIASWVLGGALVLMVGLVFSQLMRFKSEAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C92</accession>
    <name>Q97C92_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipid/polyisoprenoid-binding YceI-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0219791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916467.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C92"/>
    <dbReference type="STRING" id="273116.gene:9380981"/>
    <dbReference type="PaxDb" id="273116-14324425"/>
    <dbReference type="GeneID" id="1440727"/>
    <dbReference type="KEGG" id="tvo:TVG0219791"/>
    <dbReference type="eggNOG" id="arCOG07357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071003_3_0_2"/>
    <dbReference type="OrthoDB" id="55966at2157"/>
    <dbReference type="PhylomeDB" id="Q97C92"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.110">
        <property type="entry name" value="Lipid/polyisoprenoid-binding, YceI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007372">
        <property type="entry name" value="Lipid/polyisoprenoid-bd_YceI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036761">
        <property type="entry name" value="TTHA0802/YceI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34406">
        <property type="entry name" value="PROTEIN YCEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34406:SF1">
        <property type="entry name" value="PROTEIN YCEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04264">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00867">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101874">
        <property type="entry name" value="YceI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipid/polyisoprenoid-binding YceI-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="177" mass="19935" checksum="72E8E49693BFC110" modified="2001-10-01" version="1">MEVLKKWTPDKAHSSIEFVVRHMMVSKVRGSFNDFDIVFIGDPDDLAQGKAEATVRVASITTGEADRDQHLRSQDFFYVEKFPEIKFSSTDIMKAGDGYKINGKLTIKDVTKDVSFEGELEGKIKDPYGKQRFGFTARTVIDRRDFGLMWNMALDNGGILVGNTVNVEISLEMVEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C96</accession>
    <name>Q97C96_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transport system ATP-binding protein P1P2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0213590</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C96"/>
    <dbReference type="STRING" id="273116.gene:9380977"/>
    <dbReference type="PaxDb" id="273116-14324421"/>
    <dbReference type="KEGG" id="tvo:TVG0213590"/>
    <dbReference type="eggNOG" id="arCOG00175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="PhylomeDB" id="Q97C96"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000042">
        <property type="entry name" value="Maltose/maltodextrin ABC transporter, ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047641">
        <property type="entry name" value="ABC_transpr_MalK/UgpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875">
        <property type="entry name" value="MALTODEXTRIN IMPORT ATP-BINDING PROTEIN MSMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875:SF15">
        <property type="entry name" value="TREHALOSE IMPORT ATP-BINDING PROTEIN SUGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="7"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="353" mass="40115" checksum="7B29805C334C8518" modified="2001-10-01" version="1">MTKMVNVRVVDVYKSYGKVQVLKGINFNIEDGEFFVLLGSSGAGKSTLLNILAGLEAVDSGSIFFDDKIVNDLPPVERGVAMVFQNYALYPHMTVYKNMAFPLKMLNYKKDSIDRIVKKVAETLDITWLLNRYPRELSGGQAQRVALGRALVRDPKIFLLDEPLSNLDANIRSKIRNELKLMQKQLNKTFIYVTHDQMEAMSLGDHIGILHNGVLEQYGRPIDIYNNPINEYIATFLGDPEINMLSCIKYRDVYECDGSIKFKLDLDDKKITLGIRPENIYLHRQNDNDIEGKIIPKVSELLGSHTLIIGSVENKEIRIVTEETNISIGKEIPIYINLNKCLIFNEKGENINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CB3</accession>
    <name>Q97CB3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aconitate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG0196114</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <reaction evidence="8">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
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        </reaction>
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            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        <location>
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        <location>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59331.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="881" mass="98339" checksum="9E6030319F99FD84" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
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            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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        </lineage>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <location>
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        <source>
            <dbReference type="PROSITE" id="PS50943"/>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="21799" checksum="41132CC5DBBC3CDC" modified="2001-10-01" version="1">MRMEMMPNIDELRKMRKNLGISQKDLARVTGVSQSYIARLEKGTINPTYSKIKAIYEYLTKSSEKANTIALTCDKIMTRNVVVCRADDSILKALNLMRDRGYSQLPVVNEENKVIGTVTESNINDMLLKGMSVDSLRGLTVRRVMGDVLPQVDKNTPINVIYQLLKYSNAVLVLDSGNLTGIITKADILKTVAGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CB7</accession>
    <name>Q97CB7_THEVO</name>
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        <submittedName>
            <fullName evidence="2">TVG0190639 protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">TVG0190639</name>
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    <organism evidence="2 3">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916440.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CB7"/>
    <dbReference type="STRING" id="273116.gene:9380955"/>
    <dbReference type="PaxDb" id="273116-14324399"/>
    <dbReference type="GeneID" id="1441670"/>
    <dbReference type="KEGG" id="tvo:TVG0190639"/>
    <dbReference type="eggNOG" id="arCOG07356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1168632_0_0_2"/>
    <dbReference type="OrthoDB" id="379778at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="237" mass="26303" checksum="F8DF9E8B6AF44326" modified="2001-10-01" version="1">MPFKEQVRKHNFASTLLGGAILSILIGYLVSYVPSLFLIVFLVPIVILVLMHYFGLYGLRKRLLYGTVIVILAALLISSAESQVYYSSEHPITATYDNGISATATVSPFSGIMPSYNFSITIVNYEHLSSQNFSLTIASPTFTLNETSLNSIAKGSDITLYYDVPAGHLPLGIYNYSFHFANYTLTGPGPINTNLGTWIADSVISVSFLYFIYYEIILLAGIFLMRSIDHSRSYNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CC0</accession>
    <name>Q97CC0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0188161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916437.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CC0"/>
    <dbReference type="STRING" id="273116.gene:9380952"/>
    <dbReference type="PaxDb" id="273116-14324396"/>
    <dbReference type="GeneID" id="1441667"/>
    <dbReference type="KEGG" id="tvo:TVG0188161"/>
    <dbReference type="eggNOG" id="arCOG00842">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_4_2"/>
    <dbReference type="OrthoDB" id="120213at2157"/>
    <dbReference type="PhylomeDB" id="Q97CC0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43328">
        <property type="entry name" value="ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43328:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="176" mass="20064" checksum="011FB30A9557B079" modified="2001-10-01" version="1">MIVSSSRMDVTLESPIGIEYADDIARLANDRTIKRNIGSHSFPYPYTKEDAIFFIEAERGYGKEAFRIDFLIKYKGGPAGIIGLSEIDRTDKKAHVGYWIGKKFRGKGIATESLRLIVEYAKNDGFHRLYTSVIEGNYPSMIVLMRNGFSIEGVEKDSIRIGNRYYDFINFGKLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CF9</accession>
    <name>Q97CF9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0147946</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_162009528.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CF9"/>
    <dbReference type="PaxDb" id="273116-14324356"/>
    <dbReference type="GeneID" id="1441626"/>
    <dbReference type="KEGG" id="tvo:TVG0147946"/>
    <dbReference type="eggNOG" id="arCOG03883">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_213586_0_0_2"/>
    <dbReference type="OrthoDB" id="144464at2157"/>
    <dbReference type="PhylomeDB" id="Q97CF9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019205">
        <property type="entry name" value="DUF2080_transposon-encoded"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033496">
        <property type="entry name" value="DUF2080_fam_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09853">
        <property type="entry name" value="DUF2080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="52" mass="5876" checksum="9C4DE7C84A1A1636" modified="2001-10-01" version="1">MKEANEITAHNIEAYMERTVTKFGSGAKVDCPKEYLGKRVCLIIKKDDEGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CG4</accession>
    <name>Q97CG4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0144477 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0144477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916391.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CG4"/>
    <dbReference type="STRING" id="273116.gene:9380907"/>
    <dbReference type="PaxDb" id="273116-14324351"/>
    <dbReference type="GeneID" id="1441621"/>
    <dbReference type="KEGG" id="tvo:TVG0144477"/>
    <dbReference type="HOGENOM" id="CLU_1623552_0_0_2"/>
    <dbReference type="OrthoDB" id="374223at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13470">
        <property type="entry name" value="PIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="2"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="163" mass="18576" checksum="0FDF7CA380E40AF5" modified="2001-10-01" version="1">MRLIIDTNVVLAYLISGSARSSNAKVCRRVVNGLDKAFTCDILYGELKRLLDLDPDLIEKISIIGKKEGDVFRALYNAKTEYLNSRLDPYVMQLNFVKTENLAIDPVAIKDIGNDWYMISIAKSVSIDYIVTWNTQDVLKYADRNGIDVNRILEPPKYLELIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CG8</accession>
    <name>Q97CG8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lipid transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0142182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CG8"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="SCP-x_thiolase"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Thiolase"/>
    </dbReference>
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        <location>
            <begin position="5"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="247"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="387" mass="41615" checksum="AEFBB6E42852A0B0" modified="2001-10-01" version="1">MSNDVYIIGAGETKFGELWDKSLRDLAVEAGLEAIKDANIYSKDLQMLYASNSLAGTINEQSNIAALAADFSGIAENHVPAVRVEASTASGGAAVREAYLAIKSGEYDVVMVGGVEKMTDIYGTEIIDVQSSILDREWESFNGATPAAMAAIAARRYMHDFNVEREAISMVAVNDHANASLNPDAQYRNKITVDQVLNSDPVAEPLNVFDCSPITDGASAIILASEGFVKKNRIDGVRILSSAMAEDYLALHSRKSIYTLESTKIATEEALKKAGIKREDVSFLELNDSYSIYGLLELEDMGFAEKGKAKDLVYDDIKLNGRLPVNPSGGLKAKGNPLGATGVSQFFEAYLQLKGKAGQRQVKDARYALLHNMAGTGATSVVHVVGE</sequence>
</entry>
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    <accession>Q97CI5</accession>
    <name>Q97CI5_THEVO</name>
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        <submittedName>
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    </protein>
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        <name evidence="1" type="ORF">TVG0124725</name>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="GeneID" id="1441602"/>
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        <property type="component" value="Chromosome"/>
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    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR012675">
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    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053833">
        <property type="entry name" value="SAMP2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005028">
        <property type="entry name" value="PRK06437.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21965">
        <property type="entry name" value="SAMP2"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="64" mass="7019" checksum="68FFE18DC543BD9D" modified="2001-10-01" version="1">MITVKGHIKKSVEIDREMSVGDILKDLGLPEEEYVVIVNGKPVLADHTVKKDDDVVILEVFSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CI6</accession>
    <name>Q97CI6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome assembly chaperone family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0124526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
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    <dbReference type="AlphaFoldDB" id="Q97CI6"/>
    <dbReference type="STRING" id="273116.gene:9380883"/>
    <dbReference type="PaxDb" id="273116-14324329"/>
    <dbReference type="GeneID" id="1441601"/>
    <dbReference type="KEGG" id="tvo:TVG0124526"/>
    <dbReference type="eggNOG" id="arCOG00347">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075000_0_0_2"/>
    <dbReference type="OrthoDB" id="35908at2157"/>
    <dbReference type="PhylomeDB" id="Q97CI6"/>
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        <property type="component" value="Chromosome"/>
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        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
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        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF7">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
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        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="204"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="27206" checksum="FCA65300EC3CEBBE" modified="2001-10-01" version="1">MVSKKSSESQVIEIEKRSYNNPIVICGFASSTPTGVLTASYMIETLGLHQVAHLISQHVPPVTVFVGGKLRHPFRIYANNSNTLLVAVCEVPILTSHIYEISNALMSWLEEIGASEIAVIEGSPVSGIPEDRNVYSVAEKRRLDKLKKVGIPAAESALIAGMGGGMLNECLVRRMSGISFITPTSADIPDPGAVLSVVNALNKVYNLNIETDLLEQQVKELNDQIKKIEEQYKELQNRQKQEPQSMYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CJ2</accession>
    <name>Q97CJ2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR system Cms endoribonuclease Csm3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CRISPR type III A-associated RAMP protein Csm3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0114661</name>
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    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
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    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="journal article" date="2019" name="J. Mol. Biol." volume="431" first="748" last="763">
            <title>Crystal Structures of Csm2 and Csm3 in the Type III-A CRISPR-Cas Effector Complex.</title>
            <authorList>
                <person name="Takeshita D."/>
                <person name="Sato M."/>
                <person name="Inanaga H."/>
                <person name="Numata T."/>
            </authorList>
            <dbReference type="PubMed" id="30639408"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2019.01.009"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS)</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated Csm3 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916366.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="6AE2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=1-229"/>
    </dbReference>
    <dbReference type="PDBsum" id="6AE2"/>
    <dbReference type="AlphaFoldDB" id="Q97CJ2"/>
    <dbReference type="SMR" id="Q97CJ2"/>
    <dbReference type="STRING" id="273116.gene:9380877"/>
    <dbReference type="PaxDb" id="273116-14324323"/>
    <dbReference type="DNASU" id="1441596"/>
    <dbReference type="GeneID" id="1441596"/>
    <dbReference type="KEGG" id="tvo:TVG0114661"/>
    <dbReference type="eggNOG" id="arCOG02658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067743_0_0_2"/>
    <dbReference type="OrthoDB" id="44077at2157"/>
    <dbReference type="PhylomeDB" id="Q97CJ2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09684">
        <property type="entry name" value="Csm3_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013412">
        <property type="entry name" value="CRISPR-assoc_RAMP_Csm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052216">
        <property type="entry name" value="CRISPR_Csm3_endoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02582">
        <property type="entry name" value="cas7_TM1809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579">
        <property type="entry name" value="CRISPR SYSTEM CMS ENDORIBONUCLEASE CSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579:SF3">
        <property type="entry name" value="CRISPR SYSTEM CMS ENDORIBONUCLEASE CSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="12" id="KW-0002">3D-structure</keyword>
    <keyword evidence="7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="9">
        <location>
            <begin position="15"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="6AE2"/>
        </source>
    </evidence>
    <sequence length="229" mass="25531" checksum="2009F8BD83B8F09D" modified="2001-10-01" version="1">MSDSKWKYNIIYNMEIEVLTGLHIGGSNEELKIGGTDSPVITTKYLINNVEPCDLPYIPGSSIKGKIRSLLENVDYKGKNGDDIVSKMFGYYPNAGEGIKRLTRLIIRDAFLDDGHIKSAEDARNVIEIKSENKIDPIESKATPRFIERVRRGTKFKGKIILSIYEGDNEEEMIKCLKTGISLLEDSYLGGNGTRGYGSVKITLGEPIKKGIDKYEENIKDNTSNGSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CL1</accession>
    <name>Q97CL1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate formimidoyltransferase</fullName>
            <ecNumber evidence="3">2.1.2.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0092631</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.2.5" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916347.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CL1"/>
    <dbReference type="STRING" id="273116.gene:9380858"/>
    <dbReference type="PaxDb" id="273116-14324304"/>
    <dbReference type="GeneID" id="1441577"/>
    <dbReference type="KEGG" id="tvo:TVG0092631"/>
    <dbReference type="eggNOG" id="arCOG05394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040037_0_0_2"/>
    <dbReference type="OrthoDB" id="56032at2157"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00555"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005542">
        <property type="term" value="F:folic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030409">
        <property type="term" value="F:glutamate formimidoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.670">
        <property type="entry name" value="Formiminotransferase, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.990.10">
        <property type="entry name" value="Formiminotransferase, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013802">
        <property type="entry name" value="Formiminotransferase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037070">
        <property type="entry name" value="Formiminotransferase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004227">
        <property type="entry name" value="Formiminotransferase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012886">
        <property type="entry name" value="Formiminotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037064">
        <property type="entry name" value="Formiminotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022384">
        <property type="entry name" value="FormiminoTrfase_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051623">
        <property type="entry name" value="FTCD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02024">
        <property type="entry name" value="FtcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12234">
        <property type="entry name" value="FORMIMINOTRANSFERASE-CYCLODEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12234:SF8">
        <property type="entry name" value="FORMIMINOTRANSFERASE-CYCLODEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02971">
        <property type="entry name" value="FTCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07837">
        <property type="entry name" value="FTCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01221">
        <property type="entry name" value="FTCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01222">
        <property type="entry name" value="FTCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55116">
        <property type="entry name" value="Formiminotransferase domain of formiminotransferase-cyclodeaminase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0290">Folate-binding</keyword>
    <keyword evidence="6" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formiminotransferase N-terminal subdomain" evidence="9">
        <location>
            <begin position="2"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Formiminotransferase C-terminal subdomain" evidence="8">
        <location>
            <begin position="181"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="302" mass="33597" checksum="958B2C62EE9185CB" modified="2001-10-01" version="1">MTLIECVPNFSEGRDKSKVDQIKSAISAIPTVRILDVEMDSNHNRSVITFVCDDGKAVEAAFAGIKKAAELIDMDKHTGEHPRFGAADVIPFVPLDDTKMSRCVQLARELGKRVGDELNIPVFLYAEAATRPERADLAAIRNKSFQYEQLKGAIKEEKWKPDFGPSEVGKAGASIIGARDFLIAYNVNLNISDVEIGKKIASALRARDGGLTFVKALAFYLKDRNIVQISMNLTNFRKTPIYRAYELVKLEASRYGAYPIESEIVGLVPEQALIDAAKFYLQLNGFDEHNLIERKINENVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CM0</accession>
    <name>Q97CM0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome d ubiquinol oxidase subunit II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0080863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916338.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CM0"/>
    <dbReference type="STRING" id="273116.gene:9380847"/>
    <dbReference type="PaxDb" id="273116-14324295"/>
    <dbReference type="GeneID" id="1441568"/>
    <dbReference type="KEGG" id="tvo:TVG0080863"/>
    <dbReference type="eggNOG" id="arCOG05350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876093_0_0_2"/>
    <dbReference type="OrthoDB" id="56361at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="35009" checksum="FDC0E184FA12D42D" modified="2001-10-01" version="1">MNGVFAINFFVMALMLALYISESFGAIILLFFYKDGSKKVTPYVVPIWELTGTFIAFWVVTSDFAYPSILVPAAFIYAVPLLILLIAFIGRNAFIIFGEFIKKGFIDEKAMYKIFSIFECIFCLVFVIFASSVVTGGGVILSKLAFNVGKWATTPGDVLFVVGTVIFIFGLSFIFYGLEKYKLHSIVLTYIGIILAVVAFVQIHLSISTVPLSPVLAIPILIGILAPILFYIDSTRKIVENKIFFLALLVISVYPFYSLVYPKFFGGTLPVSAFLTNSPMATAYFYITLGGGILLIAMLAYMVYVANGFNKMVAEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CM7</accession>
    <name>Q97CM7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glucose 1-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0075046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2012-06" db="PDB data bank">
            <title>Structure of Thermoplasma volcanium aldohexose dehydrogenase.</title>
            <authorList>
                <person name="Yasutake Y."/>
                <person name="Nishioka T."/>
                <person name="Tamura T."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS)</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916331.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="3VTZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D=1-261"/>
    </dbReference>
    <dbReference type="PDBsum" id="3VTZ"/>
    <dbReference type="AlphaFoldDB" id="Q97CM7"/>
    <dbReference type="SMR" id="Q97CM7"/>
    <dbReference type="STRING" id="273116.gene:9380840"/>
    <dbReference type="PaxDb" id="273116-14324288"/>
    <dbReference type="GeneID" id="1441561"/>
    <dbReference type="KEGG" id="tvo:TVG0075046"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_2"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="PhylomeDB" id="Q97CM7"/>
    <dbReference type="EvolutionaryTrace" id="Q97CM7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005007">
        <property type="entry name" value="PRK06398.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321:SF8">
        <property type="entry name" value="ESTRADIOL 17-BETA-DEHYDROGENASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3VTZ"/>
        </source>
    </evidence>
    <sequence length="261" mass="28195" checksum="C4FB39E7F5DE43D3" modified="2001-10-01" version="1">MEEFTDKVAIVTGGSSGIGLAVVDALVRYGAKVVSVSLDEKSDVNVSDHFKIDVTNEEEVKEAVEKTTKKYGRIDILVNNAGIEQYSPLHLTPTEIWRRIIDVNVNGSYLMAKYTIPVMLAIGHGSIINIASVQSYAATKNAAAYVTSKHALLGLTRSVAIDYAPKIRCNAVCPGTIMTPMVIKAAKMEVGEDENAVERKIEEWGRQHPMGRIGRPEEVAEVVAFLASDRSSFITGACLTVDGGLLSKLPISTPNADNSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CM8</accession>
    <name>Q97CM8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">mannonate dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG0075057</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the dehydration of D-mannonate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O</text>
            <dbReference type="Rhea" id="RHEA:20097"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17767"/>
            <dbReference type="ChEBI" id="CHEBI:57990"/>
            <dbReference type="EC" id="4.2.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate metabolism; pentose and glucuronate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mannonate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.8" evidence="7"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916330.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CM8"/>
    <dbReference type="SMR" id="Q97CM8"/>
    <dbReference type="STRING" id="273116.gene:9380839"/>
    <dbReference type="PaxDb" id="273116-14324287"/>
    <dbReference type="DNASU" id="1441560"/>
    <dbReference type="GeneID" id="1441560"/>
    <dbReference type="KEGG" id="tvo:TVG0075057"/>
    <dbReference type="eggNOG" id="arCOG05324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058621_0_0_2"/>
    <dbReference type="OrthoDB" id="39900at2157"/>
    <dbReference type="PhylomeDB" id="Q97CM8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008927">
        <property type="term" value="F:mannonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042840">
        <property type="term" value="P:D-glucuronate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004628">
        <property type="entry name" value="Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30387">
        <property type="entry name" value="MANNONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30387:SF2">
        <property type="entry name" value="MANNONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03786">
        <property type="entry name" value="UxuA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016049">
        <property type="entry name" value="Man_dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="333" mass="37655" checksum="B5F79C097DC15D10" modified="2001-10-01" version="1">MHASVKFRIAEIINSTMPSAEWRLLKQIGVNEVVGTLPRDPSDWRGDQPDLPWDYAPLSSLKRRLSEYGLTLSAIEDNPPMEKIKYGVKGKEDELENVLRLIENMGRLKIGVWCYNWMAGQGWMRSRISIPDRGGAEVTGFSLSDIEEYPPPKMGKIDAETLWKNLAWFLDNVIPVAEDSGVKIAMHPDDPPLEEIRGVSRIMNNVKAFDKLIEMKRSEYNTITLCQGNFTLMTEDLPSAIRHFVRKKAVSFVHFRDVKGKKDNFIESFIDDGKTDTASCMSAYIEEGFDGIMRTDHTPTLYGDTYKVPGYSILGRLHAIGYIQGLIDGVSMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CN4</accession>
    <name>Q97CN4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF35 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0067028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CN4"/>
    <dbReference type="STRING" id="273116.gene:9380833"/>
    <dbReference type="PaxDb" id="273116-14324281"/>
    <dbReference type="DNASU" id="1441554"/>
    <dbReference type="KEGG" id="tvo:TVG0067028"/>
    <dbReference type="eggNOG" id="arCOG01283">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_2_1_2"/>
    <dbReference type="PhylomeDB" id="Q97CN4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF6">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="37"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="78"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="17509" checksum="C0DA58B65508FC5E" modified="2001-10-01" version="1">MPEYISKDEKSGYPILVDMRSLSIKYRIPVLKIKKFFDALDKGIIMATRCPVCGAKYFPPEAWCSACGYSGEMEFYPIETDGTLLTFTKIFAKPGSFSSFPDYTVGIARIDKEGFNVLAWLSDKIENPKVGMPVKLRVVKRAIDNALIYEISNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CP3</accession>
    <name>Q97CP3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NDP-sugar epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0060866</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916315.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CP3"/>
    <dbReference type="STRING" id="273116.gene:9380823"/>
    <dbReference type="PaxDb" id="273116-14324272"/>
    <dbReference type="GeneID" id="1441545"/>
    <dbReference type="KEGG" id="tvo:TVG0060866"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="PhylomeDB" id="Q97CP3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05234">
        <property type="entry name" value="UDP_G4E_2_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="312" mass="34604" checksum="57846630EF1F4FB1" modified="2001-10-01" version="1">MENKRILITGGAGFIGSNMVEHLLPKNEVTVIDNLSITDDRYIKKFYDNPNFKFIKKDILNGIDGYHYDIVVHLAADSDVRNGSSNPALDMKVNVEGTISVLEMMRKSDIKDILFASSSTVYGEAKVIPTPENYGPLLPISSYGASKLAAEAFISAYASYYGFNALLFRFANVVGKNSTHGVIFDFINKLKNNKKELEVLGDGTQAKSYIHVEDLIGSMIYVYEHVKGVEPFNLGNDDVTSVDKIAKFVLEAMGLKDTAIVHRGGYNGRGWPGDVKYAMMDIAKLKKTGWKNKYSSDEAVKKSINEVMNQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CR2</accession>
    <name>Q97CR2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0044090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CR2"/>
    <dbReference type="PaxDb" id="273116-14324253"/>
    <dbReference type="KEGG" id="tvo:TVG0044090"/>
    <dbReference type="eggNOG" id="arCOG03350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161402_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CR2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054581">
        <property type="entry name" value="EncFtn-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22277">
        <property type="entry name" value="EncFtn-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0409">Iron storage</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="115" mass="13821" checksum="21C206431DCD0F09" modified="2001-10-01" version="1">MILCYLSRLRKCIILTSHCFNMPRYESGEDLSERIKDLSRARQSLIEEIEAMMFYDERADATKDEDLKYIMEHNRDDEKEHAALLLEWIRRHDPAMDKELHEILFSNKKMKELGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CT1</accession>
    <name>ACYP_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Acylphosphatase</fullName>
            <ecNumber>3.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Acylphosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">acyP</name>
        <name type="ordered locus">TV0020</name>
        <name type="ORF">TVG0019502</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acylphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916277.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CT1"/>
    <dbReference type="SMR" id="Q97CT1"/>
    <dbReference type="STRING" id="273116.gene:9380785"/>
    <dbReference type="PaxDb" id="273116-14324234"/>
    <dbReference type="GeneID" id="1441506"/>
    <dbReference type="KEGG" id="tvo:TVG0019502"/>
    <dbReference type="eggNOG" id="arCOG01674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141932_1_0_2"/>
    <dbReference type="OrthoDB" id="6643at2157"/>
    <dbReference type="PhylomeDB" id="Q97CT1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268:SF4">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00112">
        <property type="entry name" value="ACYLPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000326873" description="Acylphosphatase">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Acylphosphatase-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="90" mass="10532" checksum="BEA50B3AC5A81A56" modified="2001-10-01" version="1">MLTTRRVRFYGRVQGINFRSNTLVKALELGVKGWIKNLPDGSVEALFSGESEQIEKLISYCVSNMPYAEVKRYDVYIEPYTEFQDFQIKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>P58274</accession>
    <name>LONB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Archaeal Lon protease</fullName>
            <ecNumber>3.4.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>ATP-dependent protease La homolog type 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">TV0493</name>
        <name type="ORF">TVG0479569</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S16 family. Archaeal LonB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916752.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58274"/>
    <dbReference type="SMR" id="P58274"/>
    <dbReference type="STRING" id="273116.gene:9381275"/>
    <dbReference type="MEROPS" id="S16.A11"/>
    <dbReference type="PaxDb" id="273116-14324708"/>
    <dbReference type="GeneID" id="1441010"/>
    <dbReference type="KEGG" id="tvo:TVG0479569"/>
    <dbReference type="eggNOG" id="arCOG02160">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392630_0_0_2"/>
    <dbReference type="OrthoDB" id="64652at2157"/>
    <dbReference type="PhylomeDB" id="P58274"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004663">
        <property type="entry name" value="Lon_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046843">
        <property type="entry name" value="LonB_AAA-LID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00764">
        <property type="entry name" value="lon_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20436">
        <property type="entry name" value="LonB_AAA-LID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000076155" description="Archaeal Lon protease">
        <location>
            <begin position="1"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="167"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="3">
        <location>
            <begin position="433"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="525"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="568"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="655" mass="71341" checksum="9DA7A252DA261C1D" modified="2001-09-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-07-19" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q6I813</accession>
    <name>Q6I813_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pseudomonapepsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1176557</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q6I813"/>
    <dbReference type="STRING" id="273116.gene:9381945"/>
    <dbReference type="PaxDb" id="273116-14325384"/>
    <dbReference type="KEGG" id="tvo:TVG1176557"/>
    <dbReference type="eggNOG" id="arCOG03665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_286786_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q6I813"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017001">
        <property type="entry name" value="Pept_S53_physarolisin-II_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032623">
        <property type="entry name" value="Peptidase_SSO2181_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
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    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1107"/>
            <end position="1127"/>
        </location>
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    <feature type="domain" description="Peptidase S53" evidence="6">
        <location>
            <begin position="175"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="1139" mass="126072" checksum="442F0EBDADC2DD8B" modified="2004-07-19" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
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        <name evidence="2" type="ORF">TVG1552703</name>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="tvo:TVG1552703"/>
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    <dbReference type="OrthoDB" id="75806at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="425"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="974" mass="112697" checksum="F73BECB3DE57F898" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q978C5</accession>
    <name>Q978C5_THEVO</name>
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        <recommendedName>
            <fullName evidence="3">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">TVG1545079</name>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="29400" checksum="35FA882BCEE791B6" modified="2001-10-01" version="1">MSRILSSEPDRLTEPEFLAKALKENKYVPYYYINVRENPTSIKIYRYVTKISNRDNDIDEVKNKFDSFLYGKSIVILDEVDFLQDYDVLYHITRHTKANLILLTQKVYWYMDTNDESVKSSLQPDHIVFYEYSSDEIREILKMRADEGLNKYDPDSLGLLSAMLVRNYRSDARIGIKALEIIGRMNEWDDDSVRTALKQAYVEVEGETLKNLGDRDLLILAALIKNQDTNRAYSEVSASSNLLLRGVSKSTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q978D0</accession>
    <name>Q978D0_THEVO</name>
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        </submittedName>
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    <organism evidence="1 2">
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978D0"/>
    <dbReference type="STRING" id="273116.gene:9382301"/>
    <dbReference type="PaxDb" id="273116-14325726"/>
    <dbReference type="GeneID" id="1442176"/>
    <dbReference type="KEGG" id="tvo:TVG1537727"/>
    <dbReference type="HOGENOM" id="CLU_1431719_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="189" mass="21806" checksum="8DF0655BA9AEDB4F" modified="2001-10-01" version="1">MGIAIFQRFRKSGLKGVFQSHFQNFFEERFPDYILEMIPITPDEVLDAFKNGVIKSVQLISHSLTSDLADYYSTEDDKRGRVILGFEKGRFHGKFIDNFIKIVKHEMPISKIIESEWMSPDEIKTEVEIKGTQKTFTLKEEGSEITPGIDISKKVSYGNDGFPTQESVNREALKYLEILKSHLRGRNEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q978F2</accession>
    <name>Q978F2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Extradiol ring-cleavage dioxygenase class III enzyme subunit B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1514647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917695.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978F2"/>
    <dbReference type="STRING" id="273116.gene:9382277"/>
    <dbReference type="PaxDb" id="273116-14325704"/>
    <dbReference type="GeneID" id="1442155"/>
    <dbReference type="KEGG" id="tvo:TVG1514647"/>
    <dbReference type="eggNOG" id="arCOG01729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089900_0_0_2"/>
    <dbReference type="OrthoDB" id="55814at2157"/>
    <dbReference type="PhylomeDB" id="Q978F2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07952">
        <property type="entry name" value="ED_3B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B" evidence="1">
        <location>
            <begin position="21"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="253" mass="28923" checksum="1C0B1E31B26B6A35" modified="2001-10-01" version="1">MLNRIYIIPHGDELIDMDTDEDRKMHEAIKKYSENDDSETLVVISPHGIVLDRDVGVIYSSKLSGAYRTKHHYIRKTYDNDLELADSIARSQHAQHLRCITLSGNVPCPLDFGTLIPLQFFKRKRVVAMGEPRLLDYSAIVGFADDLYSAMSKFERKISLIISADQAHTHSKDGPYGFSEDADLYDKIVIDSINNNDFHALINIDKSIVERAKPDSYQNMIVTARLLEISRRKMHVDYYYVAGYFGMLLAREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q978H8</accession>
    <name>Q978H8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1485662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978H8"/>
    <dbReference type="PaxDb" id="273116-14325676"/>
    <dbReference type="KEGG" id="tvo:TVG1485662"/>
    <dbReference type="eggNOG" id="arCOG02127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03965">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041125_0_1_2"/>
    <dbReference type="PhylomeDB" id="Q978H8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13565">
        <property type="entry name" value="HTH_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="163"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="263" mass="30543" checksum="E04E3C20589C5CD4" modified="2001-10-01" version="1">MNVYEAKIYRTVNKALLSGIDAALNDRQRPGKPRMISDEARAYIIKTACTKPIELGLSYELWTNRLLTRYIRENAPEEYNISGISNGTVSKILKKSNIKPYKITYYEEKTDPDFDVKGREILHVYKDINIYKRNNDNNNELYAFLSYDEKPGIQATGNIYNDKPPDKNHSNIARNHDYIRHGTLSLLAGIDLVTGHIIYSIEDKHKSIEFIKCLDNVDKYYPDDYKIKIILDNNSIHKSKETQEYLNKKPGRFKFVFTPVHAN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q978J5</accession>
    <name>TBP_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Box A-binding protein</fullName>
            <shortName evidence="1">BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">TATA sequence-binding protein</fullName>
            <shortName evidence="1">TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tbp</name>
        <name type="ordered locus">TV1420</name>
        <name type="ORF">TVG1467237</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917652.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978J5"/>
    <dbReference type="SMR" id="Q978J5"/>
    <dbReference type="STRING" id="273116.gene:9382229"/>
    <dbReference type="PaxDb" id="273116-14325659"/>
    <dbReference type="GeneID" id="1442110"/>
    <dbReference type="KEGG" id="tvo:TVG1467237"/>
    <dbReference type="eggNOG" id="arCOG01764">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060161_4_3_2"/>
    <dbReference type="OrthoDB" id="350539at2157"/>
    <dbReference type="PhylomeDB" id="Q978J5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04518">
        <property type="entry name" value="TBP_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000007">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000010">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033711">
        <property type="entry name" value="TBP_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001593">
        <property type="entry name" value="PRK00394.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001597">
        <property type="entry name" value="PRK00394.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001598">
        <property type="entry name" value="PRK00394.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000154030" description="TATA-box-binding protein">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="9"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="100"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00408"/>
        </source>
    </evidence>
    <sequence length="184" mass="20518" checksum="178CB12561746F5F" modified="2001-10-01" version="1">MSEREKITIENIVASTSLAEHLDLSRIALALDGSEYEPEQFPGLIYRLQDPKTAVLIFRSGKVNCTGAKNIEDVKRTIKIIIDKLKAANIEVYDDPDIIVQNIVAVYDLESELNLTDIAMSLGLENVEYEPEQFPGLVYRVEEPRVVLLLFGSGKVVCTGAKEESEIEQAVIKVKKELQKVGLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q978N4</accession>
    <name>HUTU_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable urocanate hydratase</fullName>
            <shortName evidence="1">Urocanase</shortName>
            <ecNumber evidence="1">4.2.1.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Imidazolonepropionate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hutU</name>
        <name type="ordered locus">TV1381</name>
        <name type="ORF">TVG1428912</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of urocanate to 4-imidazolone-5-propionate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-imidazolone-5-propanoate = trans-urocanate + H2O</text>
            <dbReference type="Rhea" id="RHEA:13101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
            <dbReference type="EC" id="4.2.1.49"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="1">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the urocanase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.49" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917614.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978N4"/>
    <dbReference type="SMR" id="Q978N4"/>
    <dbReference type="STRING" id="273116.gene:9382189"/>
    <dbReference type="PaxDb" id="273116-14325620"/>
    <dbReference type="GeneID" id="1442072"/>
    <dbReference type="KEGG" id="tvo:TVG1428912"/>
    <dbReference type="eggNOG" id="arCOG04670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018868_0_1_2"/>
    <dbReference type="OrthoDB" id="173478at2157"/>
    <dbReference type="PhylomeDB" id="Q978N4"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016153">
        <property type="term" value="F:urocanate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10730">
        <property type="entry name" value="Urocanase like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1770.10">
        <property type="entry name" value="Urocanase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00577">
        <property type="entry name" value="HutU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055351">
        <property type="entry name" value="Urocanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023637">
        <property type="entry name" value="Urocanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035401">
        <property type="entry name" value="Urocanase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038364">
        <property type="entry name" value="Urocanase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023636">
        <property type="entry name" value="Urocanase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035400">
        <property type="entry name" value="Urocanase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035085">
        <property type="entry name" value="Urocanase_Rossmann-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036190">
        <property type="entry name" value="Urocanase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01228">
        <property type="entry name" value="hutU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003820">
        <property type="entry name" value="PRK05414.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12216">
        <property type="entry name" value="UROCANATE HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12216:SF4">
        <property type="entry name" value="UROCANATE HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01175">
        <property type="entry name" value="Urocanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17392">
        <property type="entry name" value="Urocanase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17391">
        <property type="entry name" value="Urocanase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001423">
        <property type="entry name" value="Urocanate_hydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111326">
        <property type="entry name" value="Urocanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01233">
        <property type="entry name" value="UROCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0369">Histidine metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000207372" description="Probable urocanate hydratase">
        <location>
            <begin position="1"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="49"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="240"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="261"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="271"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00577"/>
        </source>
    </evidence>
    <sequence length="553" mass="61062" checksum="CFB008BF39BCE396" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q978P0</accession>
    <name>Q978P0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxyheptonate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1422507</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917609.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978P0"/>
    <dbReference type="STRING" id="273116.gene:9382183"/>
    <dbReference type="PaxDb" id="273116-14325614"/>
    <dbReference type="GeneID" id="1442067"/>
    <dbReference type="KEGG" id="tvo:TVG1422507"/>
    <dbReference type="eggNOG" id="arCOG01049">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062599_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978P0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052899">
        <property type="entry name" value="Class-I_DAHP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
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        <property type="entry name" value="DAHP_syn_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01361">
        <property type="entry name" value="DAHP_synth_Bsub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006421">
        <property type="entry name" value="PRK08673.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK12595.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43018">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43018:SF2">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="2">
        <location>
            <begin position="58"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="312" mass="34635" checksum="4194FC95F2E64F3B" modified="2001-10-01" version="1">MIMIADNEEIKENVVRKLEENGSDYKVVPFYEKYVIIEGNKDAGERLYSKKYRDKTVVDVGDIKIGEGLVFAAGPCSVEDEDQIIEIARSVKKAGANMLRGGAFKPRTSPYSFQGLGEEGLELLKKAKEDTGLPIVTEIMNPEYYKFFDDIDMLQVGSRNAQNFDLLRFLGRQKKPVLLKNGMGNTLKEWLEASEYIMSGGNGNVVLCYRGVRGIEEATRFSMNVGTVIAARSMTHLPMCVDPSHASGKREYVEAMTLAAVAAGADMIEIEVHNNPDCALSDSEQQVTPEVFGRIVKKAKQIKNIVSNSQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Q4</accession>
    <name>Q978Q4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-responsive regulatory protein [Lrp]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1409444</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917595.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Q4"/>
    <dbReference type="STRING" id="273116.gene:9382169"/>
    <dbReference type="PaxDb" id="273116-14325600"/>
    <dbReference type="GeneID" id="1441477"/>
    <dbReference type="KEGG" id="tvo:TVG1409444"/>
    <dbReference type="eggNOG" id="arCOG01581">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OrthoDB" id="55586at2157"/>
    <dbReference type="PhylomeDB" id="Q978Q4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="177" mass="20197" checksum="578D89BD8BC2AFAB" modified="2001-10-01" version="1">MEKKDISDQYLEGYSCDVAIRWSKIDPSLLPVDELDKQIICFLRYNARISNAEIAKALNTSEATVRRRINELVKKKIIIGFSALVDLQAVENSIKAYIIIDVDPNRMDEIAGAISDNINVLSLYRRRGKNQLIAETLFLNLEAIENFEAFISKLNGVKNFETMIISKAYRKNPWIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q978R3</accession>
    <name>Q978R3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transport system ATP-binding protein P1P2A1A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1396300</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917586.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978R3"/>
    <dbReference type="STRING" id="273116.gene:9382160"/>
    <dbReference type="PaxDb" id="273116-14325591"/>
    <dbReference type="GeneID" id="1441468"/>
    <dbReference type="KEGG" id="tvo:TVG1396300"/>
    <dbReference type="eggNOG" id="arCOG00184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="PhylomeDB" id="Q978R3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050319">
        <property type="entry name" value="ABC_transp_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776:SF7">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT ATP-BINDING PROTEIN DDPF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776">
        <property type="entry name" value="TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="7"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="85"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="477" mass="54640" checksum="EF34603DCF4BC5B1" modified="2001-10-01" version="1">MEDEIILYVSHLKKYFDIPNGLGRNLGYVKAVDDVTFTAKKGETIGIVGETGCGKTTLGRTILQLTKATDGNVYFHLDQDIMKKAIELEEEYYSLTHKKEKSEEDKRRIKEVEDELIPFRKKYSLTKMKPKQIKEYRRYMQPVFQDPFSSLDPRKLIKDIIAEPMKLLTNMSKEEILEKEKEIINEIGLSEDHLYRFPHEFSGGQRQRIGIARAISIEPDLLLLDEPTSALDVSVQAQILNMLKDIQQRHNLTYLFISHHLNVIRSMSDRVIVMYLGKIVEIAETHTLFTEMLHPYTKALLSAIPVPDPDTKKQHIILEGEIPSPVNPPKGCYFHNRCPEAMRNCGWSPRDLSEPIRDAFDVFRNPEAEALPEISKIVVDEGNNILHVRFSSQVQDQQKVLSVVQSIVDKESLGKRGIMYKAIENITWESDGKSLAIKMIEPDVPKLVEAKKDHFVSCLLYESPVKEEIENAPNPGN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q978S5</accession>
    <name>AROE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="1">SDH</shortName>
            <ecNumber evidence="1">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroE</name>
        <name type="ordered locus">TV1340</name>
        <name type="ORF">TVG1385100</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917575.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978S5"/>
    <dbReference type="SMR" id="Q978S5"/>
    <dbReference type="STRING" id="273116.gene:9382148"/>
    <dbReference type="PaxDb" id="273116-14325579"/>
    <dbReference type="GeneID" id="1441457"/>
    <dbReference type="KEGG" id="tvo:TVG1385100"/>
    <dbReference type="eggNOG" id="arCOG01033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044063_4_1_2"/>
    <dbReference type="OrthoDB" id="8744at2157"/>
    <dbReference type="PhylomeDB" id="Q978S5"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001322">
        <property type="entry name" value="PRK00258.3-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089:SF1">
        <property type="entry name" value="BIFUNCTIONAL 3-DEHYDROQUINATE DEHYDRATASE_SHIKIMATE DEHYDROGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="SHIKIMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000325186" description="Shikimate dehydrogenase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="127"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <sequence length="267" mass="29461" checksum="0A49F5B045B25664" modified="2001-10-01" version="1">MMPPDYVFGLIGHPVSHSIGQIVYNRYFQKKGLNAIYLSIDIFPETLKYFMSYASKMDGFNVTIPHKISIMDYLDQIDWEARSIGSVNLVKTEDGLLKGFNTDYYGIEYMFKKGGVDVSGKSIVVAGSGGIARTVIHYLIKNNAKSVTVKARDVKLAKNKLNAYDVDIKEYANNDYDIYINCTPLGTEAVGDPFPEVQFGKGKIAVDVVYNPPVTPFLKRAGISGSKTLSGLDLYIGQAIKTLDILTSGCDIDTLINSVRVAVNEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q978T0</accession>
    <name>Q978T0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG1377730</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978T0"/>
    <dbReference type="STRING" id="273116.gene:9382143"/>
    <dbReference type="PaxDb" id="273116-14325574"/>
    <dbReference type="KEGG" id="tvo:TVG1377730"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536743_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978T0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044065">
        <property type="entry name" value="ACP_MB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015234">
        <property type="entry name" value="DUF1936"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036311">
        <property type="entry name" value="Ta0289_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09151">
        <property type="entry name" value="DUF1936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103623">
        <property type="entry name" value="Hypothetical protein Ta0289 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51901">
        <property type="entry name" value="ACP_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="5"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="71"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="ACP-type MB" evidence="5">
        <location>
            <begin position="137"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19691" checksum="BF337E59A997704F" modified="2001-10-01" version="1">MEKIMNTNYRSVNENSSVFDAVKIMNENRLYGLIVKDNEGKDVGLISERSIIKRFIPRNKKPDEVQIKYVMRKPIPKVPSSYDVRDAAAYLSENGLERCAVVDSTGKVVGIITLTDLSRYLSRASIVDVLLSHRTKDYKHLCPKCGVGVLVPVYDQKGEIKVFRCSNPACDYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q978T8</accession>
    <name>Q978T8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1370641</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917562.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978T8"/>
    <dbReference type="STRING" id="273116.gene:9382135"/>
    <dbReference type="PaxDb" id="273116-14325566"/>
    <dbReference type="GeneID" id="1441444"/>
    <dbReference type="KEGG" id="tvo:TVG1370641"/>
    <dbReference type="eggNOG" id="arCOG01282">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_2"/>
    <dbReference type="OrthoDB" id="25212at2157"/>
    <dbReference type="PhylomeDB" id="Q978T8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000010">
        <property type="entry name" value="Acetyl-CoA acetyltransferase (Thiolase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005033">
        <property type="entry name" value="PRK06445.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF140">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE (ACETOACETYL-COA THIOLASE) (ACAB-5)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="6">
        <location>
            <begin position="273"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="396" mass="43269" checksum="EBD49698B41BBB84" modified="2001-10-01" version="1">MENENVYLVDFKRTAFSRSRPNDPERDVFNSLRMDEAMAKLINIVIDETGIRPEEINDVITGCALQQDENWTYGGRHPILLANLPDNVPGMALDRACSSSLNAITIGAMEIMTGNADIVMAGGYEHMTHVPMSNSPFLKPNVKLLVRPEYMHYDMNTGYSMGLTAEKLASLHHIGREEMDQYALRSHQLASKALDDGYFKGEIVPMEVEIGGETKIIDSDQSIRKNTSLEEMQKLRPAFKDDGVITAGNSSPLNAGASLTLLMSEKKVKEYGLKPLSRIVSFGWAGVDPSIMGEGPVPATQKALAKAGMKVEDIDYWEINEAFAVVVLNAMKELGIEENKVNIHGGGISIGHPLGATGARIAGTLSRILNEKQAEYGVATLCVGGGQGYSVIFERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q978U6</accession>
    <name>Q978U6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF357 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1359439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978U6"/>
    <dbReference type="STRING" id="273116.gene:9382127"/>
    <dbReference type="PaxDb" id="273116-14325558"/>
    <dbReference type="KEGG" id="tvo:TVG1359439"/>
    <dbReference type="eggNOG" id="arCOG01224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180506_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978U6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.90">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036809">
        <property type="entry name" value="AF1782-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023140">
        <property type="entry name" value="DUF357"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04010">
        <property type="entry name" value="DUF357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF357" evidence="1">
        <location>
            <begin position="15"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="100" mass="11638" checksum="93F80EA377EEBB4D" modified="2001-10-01" version="1">MFPLIEDELIAKVKHYIEVEEAALSKLSIAVPERSFLRKFAEDSFKMIKSYFEDAKFFYAKGDIINAFASLNYSYGWIDSGVRLGIFDGMRDQKLFTLYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q978W0</accession>
    <name>SYT_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="1">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thrS</name>
        <name type="ordered locus">TV1305</name>
        <name type="ORF">TVG1345067</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917540.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978W0"/>
    <dbReference type="SMR" id="Q978W0"/>
    <dbReference type="STRING" id="273116.gene:9382112"/>
    <dbReference type="PaxDb" id="273116-14325544"/>
    <dbReference type="GeneID" id="1441422"/>
    <dbReference type="KEGG" id="tvo:TVG1345067"/>
    <dbReference type="eggNOG" id="arCOG00401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_0_1_2"/>
    <dbReference type="OrthoDB" id="372136at2157"/>
    <dbReference type="PhylomeDB" id="Q978W0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000002">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000005">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.54.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000101116" description="Threonine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Catalytic" evidence="1">
        <location>
            <begin position="225"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="531"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01228"/>
        </source>
    </evidence>
    <sequence length="660" mass="75603" checksum="070FE6E1F4DE2A56" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q978X5</accession>
    <name>Q978X5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase</fullName>
            <ecNumber evidence="2">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1328971</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_156769188.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978X5"/>
    <dbReference type="STRING" id="273116.gene:9382096"/>
    <dbReference type="PaxDb" id="273116-14325528"/>
    <dbReference type="GeneID" id="1441406"/>
    <dbReference type="KEGG" id="tvo:TVG1328971"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="OrthoDB" id="371752at2157"/>
    <dbReference type="PhylomeDB" id="Q978X5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="8">
        <location>
            <begin position="27"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="88"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="520"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="641" mass="72025" checksum="00261E73BFD83EBC" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Y0</accession>
    <name>Q978Y0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1324343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917519.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Y0"/>
    <dbReference type="STRING" id="273116.gene:9382091"/>
    <dbReference type="PaxDb" id="273116-14325523"/>
    <dbReference type="DNASU" id="1441401"/>
    <dbReference type="GeneID" id="1441401"/>
    <dbReference type="KEGG" id="tvo:TVG1324343"/>
    <dbReference type="eggNOG" id="arCOG01806">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103221_1_0_2"/>
    <dbReference type="OrthoDB" id="56528at2157"/>
    <dbReference type="PhylomeDB" id="Q978Y0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016619">
        <property type="entry name" value="UCP014439_ACT"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014439">
        <property type="entry name" value="APE1894_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24437" checksum="522847E5DD4CDE7E" modified="2001-10-01" version="1">MNVSDLVKEYVSRHPYVMEALSEGIVNFSSLSRKIMEQYNIKSFQAVEAALKRYKPQSGEPDYKNILSRSTVEMYTNVTVIILKPKSENMKLILSLAERQSINYGRFRLIQGVQGAALVMNDNDAEKIVRSIPKGEVIQMDRKLSEIVMTSPETITFTRGYVAYLSSILSHNGINIIQIVSFYTDVTFILDPEDLVRSFNILMREIRLSQSLIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Z2</accession>
    <name>RS3A_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S3Ae</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein S1e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps3ae</name>
        <name type="ordered locus">TV1270</name>
        <name type="ORF">TVG1311805</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917505.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Z2"/>
    <dbReference type="SMR" id="Q978Z2"/>
    <dbReference type="STRING" id="273116.gene:9382076"/>
    <dbReference type="PaxDb" id="273116-14325509"/>
    <dbReference type="GeneID" id="1441387"/>
    <dbReference type="KEGG" id="tvo:TVG1311805"/>
    <dbReference type="eggNOG" id="arCOG04186">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062507_1_0_2"/>
    <dbReference type="OrthoDB" id="30639at2157"/>
    <dbReference type="PhylomeDB" id="Q978Z2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00359">
        <property type="entry name" value="Ribosomal_eS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030838">
        <property type="entry name" value="Ribosomal_eS1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003142">
        <property type="entry name" value="PRK04057.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000153564" description="Small ribosomal subunit protein eS1">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="215" mass="24426" checksum="ACE912719BB682F0" modified="2001-10-01" version="1">MAGEKFQKKGKEKWKEKVWYTVEAPPYLGSKEVSVALGEDSNSMVNRVVEVPISELTGNFKKSNEKALFRITNCEGTKCKTIFIGHYIGDDYIRRLVRRRKERIDIIEDVKTSDNSIITVKIVVVTDGKVTNTKKFQIRKVLTDFILNKGLSLPYSEFVRYLIGDDIYNDMISATKDIYPLKKIEVRKSELVSLSGISEIHAGSQNSGEEPVVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Z4</accession>
    <name>Q978Z4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1309485</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Z4"/>
    <dbReference type="STRING" id="273116.gene:9382073"/>
    <dbReference type="PaxDb" id="273116-14325507"/>
    <dbReference type="KEGG" id="tvo:TVG1309485"/>
    <dbReference type="eggNOG" id="arCOG02792">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055959_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978Z4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051084">
        <property type="entry name" value="H+-coupled_symporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43528">
        <property type="entry name" value="ALPHA-KETOGLUTARATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43528:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="1"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="398" mass="44246" checksum="916C48F81EE65FC1" modified="2001-10-01" version="1">MSASAGMFIWGIIASIAPLATAWPYVKGLPRVYLSVFLSLPSVFLLTGNTILGKLSDILGRKPIYLMTILFYLAGLLIIFASNNVVLLVIGIALSEIGVGGEEVTTLSLISEDVPIKHRGKFLAFVPNMNNVGSAVIAFVFLYFNSSSIQIQKLYFLIIAFFAVFLALYTRRSIPESFRWLRETGSEKEAENLYKKLKISEDGIRIKEPNFSVSLAFLAILGISQYLTFGLMAYIVGPYYFSPAQVSEIIFYALLGASVAGIIAMFLVDIGRKKYAILSYLGGTISILLILATIKYVDNMLVFLPLLFINMMFSEFAWASRTTLEPELFGTKRRSTSIGLVRVFPILAYIASIYLTASFTLSQYIFFNLALWCLGLVAAIVWMFIGVETKNVSMDYYS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Z5</accession>
    <name>SYC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="1">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cysS</name>
        <name type="ordered locus">TV1267</name>
        <name type="ORF">TVG1307912</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60409" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60409.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Z5"/>
    <dbReference type="SMR" id="Q978Z5"/>
    <dbReference type="STRING" id="273116.gene:9382072"/>
    <dbReference type="PaxDb" id="273116-14325506"/>
    <dbReference type="KEGG" id="tvo:TVG1307912"/>
    <dbReference type="eggNOG" id="arCOG00486">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_0_1_2"/>
    <dbReference type="OrthoDB" id="9445at2157"/>
    <dbReference type="PhylomeDB" id="Q978Z5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000130">
        <property type="entry name" value="Cysteine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000159549" description="Cysteine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="265"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="454" mass="53231" checksum="F0DE8FFAB2177E89" modified="2002-05-27" version="2">MLIYNTLTRRLQEFNEMHRGRVNLFVCGPTVQDHFHIGHARTYIFFDAVAKFLKLEGYSVFYLQNITDIDDKIINRAKEMGIEPSEVAKIYFSEFQEDMKRLKVDSVNFFARATLYIDEIVSQISRMMEKGYAYETDDGVYFEVRKFKDYGELSNQSLDQIIAGYRVAVNENKRNPEDFVLWKKKKAGEPSWPSPWGEGRPGWHIEDTAITETYFGDEYDIHGGGSDLIFPHHEAEIAQMRSISGKRYLAHYWIHTGMININKEKMSKSLKNFITIRDILKDYRPEDLRFAILNANYRTQIEFSKELMEESKKLIDYINDTYRKLEYVNGSGNFKIDVNSVISEMRSLAENDFDFHSVIVKLLAITGEINRNFEGINKEVAEELKKVYLWVDTFLGILEQKKEVSKGIVDDLVELRTRMRKEKNFLISDAIRDVLKKNGIHIEDRGDVTVWWQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Z7</accession>
    <name>Q978Z7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1306680 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1306680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Z7"/>
    <dbReference type="PaxDb" id="273116-14325504"/>
    <dbReference type="KEGG" id="tvo:TVG1306680"/>
    <dbReference type="eggNOG" id="arCOG05278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200908_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q978Z7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009409">
        <property type="entry name" value="DUF1059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06348">
        <property type="entry name" value="DUF1059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="64" mass="7241" checksum="9FD36130725C5A44" modified="2001-10-01" version="1">MLFMAKYVFKCSDIGMNCGFEASAKSIDELMPKIVEHAKTAHNITEINEDLKNKVTSAIKKKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Z9</accession>
    <name>Q978Z9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Aldehyde ferredoxin oxidoreductase [AOR]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1304136</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>tungstopterin</name>
            <dbReference type="ChEBI" id="CHEBI:30402"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917497.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Z9"/>
    <dbReference type="STRING" id="273116.gene:9382068"/>
    <dbReference type="PaxDb" id="273116-14325501"/>
    <dbReference type="GeneID" id="1441379"/>
    <dbReference type="KEGG" id="tvo:TVG1304136"/>
    <dbReference type="eggNOG" id="arCOG00709">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020364_1_0_2"/>
    <dbReference type="OrthoDB" id="30771at2157"/>
    <dbReference type="PhylomeDB" id="Q978Z9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013985">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF0">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase N-terminal" evidence="9">
        <location>
            <begin position="4"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="624" mass="68554" checksum="7F44898269CFF7A8" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q979B1</accession>
    <name>G1PDH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-1-phosphate dehydrogenase [NAD(P)+]</fullName>
            <shortName evidence="1">G1P dehydrogenase</shortName>
            <shortName evidence="1">G1PDH</shortName>
            <ecNumber evidence="1">1.1.1.261</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enantiomeric glycerophosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">sn-glycerol-1-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">egsA</name>
        <name type="ordered locus">TV1251</name>
        <name type="ORF">TVG1288826</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>sn-glycerol 1-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>sn-glycerol 1-phosphate + NADP(+) = dihydroxyacetone phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerol-1-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60393" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.1.1.261" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60393.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917485.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979B1"/>
    <dbReference type="SMR" id="Q979B1"/>
    <dbReference type="STRING" id="273116.gene:9382056"/>
    <dbReference type="PaxDb" id="273116-14325489"/>
    <dbReference type="DNASU" id="1441367"/>
    <dbReference type="GeneID" id="1441367"/>
    <dbReference type="KEGG" id="tvo:TVG1288826"/>
    <dbReference type="eggNOG" id="arCOG00982">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038362_0_0_2"/>
    <dbReference type="OrthoDB" id="8656at2157"/>
    <dbReference type="PhylomeDB" id="Q979B1"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106357">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106358">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08173">
        <property type="entry name" value="Gro1PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00497_A">
        <property type="entry name" value="G1P_dehydrogenase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023002">
        <property type="entry name" value="G1P_dehydrogenase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002022">
        <property type="entry name" value="PRK00843.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616:SF5">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000157356" description="Glycerol-1-phosphate dehydrogenase [NAD(P)+]">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="99"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="121"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="352" mass="38482" checksum="2D122A30CE231536" modified="2001-12-05" version="2">MEFQKYRTMHFPRDVYIGHDVLNRVLDVVDQNSRTRDVIIVTGNTTYELAGKKIVEILASSPYEVHLSFAGEANYENLKKIEEETNDVNAGIIIGVGGGTKIDLAKKLAYDKNLPFISIPTSPSHDGIASPRASLRRNGISYSEEGAMPIGVIADTSVMIKAPYRYLAAGAADVISNISAVKDWKLAHRLRGEEFSSSAAAMSEYSAQEVISQVGEIRKYDESSVWLVTKNILASGTAMAIAGNSRPGSGSEHLFAHALEAAGVNNMLHGEMCAMGTIVSLYLHDDNWQKIRDVFESLGVSVKARDYGLKEEVVIEALRRAHAIRPERYTILGESDMSYDAAVKALELTGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q979D5</accession>
    <name>Q979D5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG1262718 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1262718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917461.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979D5"/>
    <dbReference type="PaxDb" id="273116-14325464"/>
    <dbReference type="GeneID" id="1441343"/>
    <dbReference type="KEGG" id="tvo:TVG1262718"/>
    <dbReference type="eggNOG" id="arCOG07418">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1665420_0_0_2"/>
    <dbReference type="OrthoDB" id="116755at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055767">
        <property type="entry name" value="DUF7343"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24034">
        <property type="entry name" value="DUF7343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7343" evidence="2">
        <location>
            <begin position="100"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="167" mass="18864" checksum="4E6BC2C3F56313B7" modified="2001-10-01" version="1">MNSKLDDGDARYDNSRLILFAVIAVFSIIVLSITSSMMVMTIEIGYKINALEYLEYFIVILVNALMLGLSLNMIFKRYRFVVYPKLAAGIENTVSGNLLLKDNEKLIVSILEKHGGIVLQNVLVRESGLSAPTVSRILFSLENYGLIERQRKGMTNEIRLKVDNIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q979E0</accession>
    <name>Q979E0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Benzoylformate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1255890</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917455.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979E0"/>
    <dbReference type="STRING" id="273116.gene:9382026"/>
    <dbReference type="PaxDb" id="273116-14325459"/>
    <dbReference type="GeneID" id="1441337"/>
    <dbReference type="KEGG" id="tvo:TVG1255890"/>
    <dbReference type="eggNOG" id="arCOG03656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_2"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="PhylomeDB" id="Q979E0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02002">
        <property type="entry name" value="TPP_BFDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="4">
        <location>
            <begin position="183"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="5">
        <location>
            <begin position="371"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="513" mass="57632" checksum="200C81A140C83A92" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q979G7</accession>
    <name>Q979G7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1222485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917427.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979G7"/>
    <dbReference type="STRING" id="273116.gene:9381995"/>
    <dbReference type="PaxDb" id="273116-14325432"/>
    <dbReference type="GeneID" id="1441309"/>
    <dbReference type="KEGG" id="tvo:TVG1222485"/>
    <dbReference type="eggNOG" id="arCOG03660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726883_0_0_2"/>
    <dbReference type="OrthoDB" id="371889at2157"/>
    <dbReference type="PhylomeDB" id="Q979G7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052714">
        <property type="entry name" value="MFS_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531:SF1">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="11"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="404" mass="44526" checksum="5C2836346703A4E4" modified="2001-10-01" version="1">MPKFSRSTVLTIILILAVTFTMRMTTNMLMTSIPVLTKFTLLTTTFLTSLPVALYAVTGLLANLFINGRINNGMIGKAIAISLLVMAFTMPLYFMASNVWEVSAISALDGVSMGLVPPLLLTMISLSYDEVRDNVLGFYTSALSISLIVGTFLQGYLTSSVSVSVRSLFLYFFPVPLLSAILMFVIASRLKIKEDSGRSKVTTKKIVQTIPKLFKNRGFNFGFFGNLTYSFPFVIIIAYGSIIAKYYSGINPSIFFYALATFFGVSMITRLSMSFYPPKNKYDLMLASLVTTVLGFIVLIFSDNIYLFLLSMFILGFPHGSIYPIATMSVSSSVSKSEVSIAFATFNIIFNILGLLLPPAFGFIAQIVNIRVAMIVMLIPMAYIAYESLKNGSIIRRMPSSSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q979I7</accession>
    <name>Q979I7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1202787</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulator TrmB family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917407.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979I7"/>
    <dbReference type="SMR" id="Q979I7"/>
    <dbReference type="STRING" id="273116.gene:9381975"/>
    <dbReference type="PaxDb" id="273116-14325412"/>
    <dbReference type="GeneID" id="1441289"/>
    <dbReference type="KEGG" id="tvo:TVG1202787"/>
    <dbReference type="eggNOG" id="arCOG02038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062979_1_0_2"/>
    <dbReference type="OrthoDB" id="30795at2157"/>
    <dbReference type="PhylomeDB" id="Q979I7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09124">
        <property type="entry name" value="PLDc_like_TrmB_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021586">
        <property type="entry name" value="Tscrpt_reg_TrmB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11495">
        <property type="entry name" value="Regulator_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159071">
        <property type="entry name" value="TrmB C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription regulator TrmB C-terminal" evidence="3">
        <location>
            <begin position="120"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="40409" checksum="4ABA8A605FCDC837" modified="2001-10-01" version="1">MQERDWGDNTENTTIVGILQDLGLSENEAKLYMVLLQRGSMTAQDILRNMPLRQPQLYDITSSLERKGFINVLQGRPKKYKAISADVVIEAREQILKKNRDYFLNWANKTADSRREKTALINAKNLRSVVNNSIELVKEASLTLEAETTIELLEYIKEPIKRKARNGVRVELLLFGKSNIPDETIAYFKDLPVEIRYLDPGQFYVLISDEVNSVFMPRSVAIDPETPKYGYIIMDRDMSWFITHNFFVGWYKAKEITSINVNVPCLYKSQRILISDLQRMATKYSRISGTLEGVLRKNGSKFVEAGEIKGINVDTEIINFEFETSSGRYTVGGYDSQIEDVIMKSFRLTHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q979I8</accession>
    <name>Q979I8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1202803 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1202803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917406.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979I8"/>
    <dbReference type="STRING" id="273116.gene:9381974"/>
    <dbReference type="PaxDb" id="273116-14325411"/>
    <dbReference type="GeneID" id="1441288"/>
    <dbReference type="KEGG" id="tvo:TVG1202803"/>
    <dbReference type="eggNOG" id="arCOG03288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_476216_0_0_2"/>
    <dbReference type="OrthoDB" id="7795at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="585" mass="66009" checksum="5C72198545CF3502" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q979J1</accession>
    <name>Q979J1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transport system permease protein SP1P2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1198021</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917403.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979J1"/>
    <dbReference type="STRING" id="273116.gene:9381971"/>
    <dbReference type="PaxDb" id="273116-14325408"/>
    <dbReference type="GeneID" id="1441285"/>
    <dbReference type="KEGG" id="tvo:TVG1198021"/>
    <dbReference type="eggNOG" id="arCOG00159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_2"/>
    <dbReference type="PhylomeDB" id="Q979J1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43744:SF12">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MG189-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43744">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MG189-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="88"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="295" mass="33633" checksum="EF7127446FB55B37" modified="2001-10-01" version="1">MLSTGKKVYARPKRSMRPLLKKTIIYVLSIVLAMIYLLPFYWTVIKAFRNSIFANFPPNLNPLSETSLSYFLANLRTVWSFGDFPLWYFNSVFVSACVVAGSVFVGMLSGYAFAKLKFPGRNVLFYAVLATLMIPFPVISIASYVFMLDLNWLSTYQGLILPQIASALDVFIMRQYFLTIPEEMELAAKIDGLRPWQIFFSIDLPNAKPAIAAATIFSFIGSWNNFLWPLMEVHSLNMFTLPLVLNFFKGANGTQIYWNQMMTVNILTMIPTIAIFVAFERYFINGISMTFSDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q979J4</accession>
    <name>Q979J4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1194688 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1194688</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917400.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979J4"/>
    <dbReference type="STRING" id="273116.gene:9381968"/>
    <dbReference type="PaxDb" id="273116-14325405"/>
    <dbReference type="GeneID" id="1441282"/>
    <dbReference type="KEGG" id="tvo:TVG1194688"/>
    <dbReference type="HOGENOM" id="CLU_904954_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="307" mass="34360" checksum="79DBE89683343C8A" modified="2001-10-01" version="1">MVRKNMLVLLVSLLVTLSGIVVTFNIYHNDSEVISVTPLENSMMNRTDYSPSFIVNVSTSSRAVVSIYTYSSDGKNFIENFSVSGRLSITVPTNLSSSLLEAYHLQGYGLHYVYFKLKTSSVSKIVKIGIFTFHKFHFYAKYLNIDQGITDILSYVSKGNATVEWLVDGSFAGKGNNVSINIEKTGSYLLNGIISTRSFIFNATLGYIIVYPRPQVNIEIHSYKDFQNFSYLIIYANVSGGYMSYNPDSPGYPTATIYVNGSPQEHLTPSEGENIFYLSLQGYGPFFINVSVHDRFYGAKSNIIKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q979J9</accession>
    <name>Q979J9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1188322 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1188322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979J9"/>
    <dbReference type="PaxDb" id="273116-14325400"/>
    <dbReference type="KEGG" id="tvo:TVG1188322"/>
    <dbReference type="HOGENOM" id="CLU_3094338_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="51" mass="5992" checksum="0F458F361015380A" modified="2001-10-01" version="1">MAYNSRIDKNKLSKYKKFFLGIIVQDIQDNRIGENMYPSSKVILSENTCYN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q979L8</accession>
    <name>GATD_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit D</fullName>
            <shortName evidence="1">Glu-ADT subunit D</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatD</name>
        <name type="ordered locus">TV1142</name>
        <name type="ORF">TVG1168744</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparaginase 1 family. GatD subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917376.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979L8"/>
    <dbReference type="SMR" id="Q979L8"/>
    <dbReference type="STRING" id="273116.gene:9381941"/>
    <dbReference type="PaxDb" id="273116-14325380"/>
    <dbReference type="GeneID" id="1441258"/>
    <dbReference type="KEGG" id="tvo:TVG1168744"/>
    <dbReference type="eggNOG" id="arCOG01924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_2_1_2"/>
    <dbReference type="OrthoDB" id="371959at2157"/>
    <dbReference type="PhylomeDB" id="Q979L8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00586">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011878">
        <property type="entry name" value="GatD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037222">
        <property type="entry name" value="GatD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02153">
        <property type="entry name" value="gatD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003217">
        <property type="entry name" value="PRK04183.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500175">
        <property type="entry name" value="Glu_ADT_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141300">
        <property type="entry name" value="GatD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140067" description="Glutamyl-tRNA(Gln) amidotransferase subunit D">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase" evidence="2">
        <location>
            <begin position="68"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01068"/>
        </source>
    </evidence>
    <sequence length="406" mass="45065" checksum="2AF83744DAC2C105" modified="2001-10-01" version="1">MQNVEIIYKNAKVRGILINEANGLITIKADNGYNLTFDRSEVEILSRVTIEEKKEKKTEVESFGTGEKSISILATGGTIASRVDYETGAVKPVSDPRLLFGGTELESKFNIRVVNVMNQLSENMKPADWIHLARKVMDETKHSSGIVVSHGTDTMSYTSSALAFMFERLAQPIIFVGSQRSSDRPSSDTKENMEGAINFAATDLGEVGIAMHKGISDGSIVLHRAVRSRKMHTSRRDAFESIDTVHLAEYTSSVRFFSDYRKAEEENLLLDHLDEKVSIIYFHPGLVASDVENMIEGKHAVVIMGTGLGHIAKDLIPVFKNFTKDGNFAIMTSQCIYGSVDMNVYSTGRELIAAGVISAGDMVPEVAYVKAMFTLGNYGREEFINVFNRNLRGEILNRLIPREVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q979R1</accession>
    <name>Q979R1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAM-dependent methyltransferase TRM5/TYW2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG1132799</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917332.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979R1"/>
    <dbReference type="STRING" id="273116.gene:9381897"/>
    <dbReference type="PaxDb" id="273116-14325337"/>
    <dbReference type="GeneID" id="1441214"/>
    <dbReference type="KEGG" id="tvo:TVG1132799"/>
    <dbReference type="eggNOG" id="arCOG10124">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_0_2_2"/>
    <dbReference type="PhylomeDB" id="Q979R1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040601">
        <property type="entry name" value="Trm5a/b_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF31">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18093">
        <property type="entry name" value="Trm5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="4">
        <location>
            <begin position="90"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="334" mass="38447" checksum="22D249135ADE5393" modified="2001-10-01" version="1">MPTSLACVIEAPMANEIIRRLMIEDLIRKDLKIKKIENEIFIPVKDGTNLQQCRIVYSDFDERHYENIKQTMASFIKRNGGDERSLPDKWIKLGDAIFVKELIDQTTFEAFERFMGVRRAYLYESVRGVERIPVVRHLYGLRGEIRHVEDGLTYFFDPEKIMFSAGNTNERTRIREMKLDGMTVLDMFAGIGYFTLPAVKYGHAEHTDACDINPEAIKFLKKNLSANGISKSVKPICGDARIACPIKAYDLIIMGNFKSIEYLPAALIRSKAGTKIILHHLVSQERLSKFIYDLENYCISLGYFTSIQEWHIVKSYSPKMFHVATTLEILKVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q979S9</accession>
    <name>Q979S9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1110889</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979S9"/>
    <dbReference type="STRING" id="273116.gene:9381877"/>
    <dbReference type="PaxDb" id="273116-14325319"/>
    <dbReference type="KEGG" id="tvo:TVG1110889"/>
    <dbReference type="eggNOG" id="arCOG02793">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055959_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q979S9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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    </dbReference>
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    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="61"/>
        </location>
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        </location>
    </feature>
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            <end position="149"/>
        </location>
    </feature>
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        <location>
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            <end position="186"/>
        </location>
    </feature>
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        </location>
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    </feature>
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        </location>
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        <location>
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            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="406"/>
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    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="37"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="418" mass="46372" checksum="D2443DAF0F25C7F0" modified="2001-10-01" version="1">MNLIRVMPAEGSMLFDYSFRDYDEVRKFLDTAEWNRIHTSMALTMIASFFVWGLLLVTAPLVTEWPIVPSWGYMYVLLSSPAGLLIGNVVLGRTSDLLGRKSLFMATLALGIAGILGIIFSRNFFEILASIFIAEFGFGGEETVSLAFLAEQFPLKYRGKVLVLVSNSANVGVAAISGIFIAFGYSIAIEKLIFGVMAIGGITIALYTRSRIPESLRWFYTKNSDTLIEKPQMHVIPFIVLSMFAITIVLTFALVADILGPYEYPKYTSIIPFIYGLGEAISGFAIIAFIDRIGRRLTSVIAYMGGFLSMAVFLLYLISSLPFFVFLVLLVINSFFGELGWAVREILQPELFTTIRRGSGIAAVRGIAYALYIVSIFVLNGISVFEYMVFATIIWVIGSIGSILWYSKGYETLNKSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q979W5</accession>
    <name>Q979W5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Na+/H+ antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG1070221</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979W5"/>
    <dbReference type="STRING" id="273116.gene:9381839"/>
    <dbReference type="PaxDb" id="273116-14325283"/>
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    </dbReference>
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    <dbReference type="PhylomeDB" id="Q979W5"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:proton transmembrane transport"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NA(+)/H(+) ANTIPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507:SF0">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
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            <end position="40"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB60187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="420" mass="45453" checksum="0BA2201A6784E9B7" modified="2001-10-01" version="1">MSLLLVLSAVAIPIARKMSVVEIPILIFIGLIVGPFLRLISPAFSVYLFSSFAGVGIGMIGLVIILYSESHNMNIKVLKSSFYRIASLDTIGVIITALVAAGLFSLFTGAPLIIGFLFGAIISPTDPVTLIPLFRRMKISEDISEVLIGESLFNDPVGIILVSVGIAILAPHSVYVSLFSSIANSISFYPGIVAYFLIQVAVPSVVGVAIGFAIIYLNKEINFENYLTAILLGVVIFEFTIFEGISITPFPAIIATGAIVGNFSDKGIFWQREKNFQENLSYLSTSIIFLLIGSSFTINEIEQYILLGIMLTTILIFVVRPAAVFPSILIADLGKIKMKGYYKIVAFISLVGPRGVVPVVLSTLPYVVGLSYNIPILITWGPVIGVSALFIVLFSIVFQTIYTPIMKNIFIPDQGNGNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A48</accession>
    <name>Q97A48_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator PadR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0986825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A48"/>
    <dbReference type="STRING" id="273116.gene:9381754"/>
    <dbReference type="PaxDb" id="273116-14325179"/>
    <dbReference type="GeneID" id="1442040"/>
    <dbReference type="KEGG" id="tvo:TVG0986825"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="PhylomeDB" id="Q97A48"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, PADR-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="18"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="151" mass="17138" checksum="F14E04B8224C379E" modified="2001-10-01" version="1">MFDKFNFVKRRGNLKYWILYLVSTKPMNGAEVMDEIERHSFGMWRPSPGSVYPALEQLEADGLVIRNEDGRYAATEQGKEELGITSAEFSRSPKTVSGIVDEIESMVMYLSDVMPVQPEDSKLNVTRLENVVSKINELISKWSGENADNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A61</accession>
    <name>Q97A61_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-8x methylmutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0974537</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CobH/CbiC family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A61"/>
    <dbReference type="STRING" id="273116.gene:9381741"/>
    <dbReference type="PaxDb" id="273116-14325166"/>
    <dbReference type="KEGG" id="tvo:TVG0974537"/>
    <dbReference type="eggNOG" id="arCOG02247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084703_1_1_2"/>
    <dbReference type="PhylomeDB" id="Q97A61"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="entry name" value="Cobalamin biosynthesis CobH/CbiC, precorrin-8X methylmutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="Precorrin-8X methylmutase CbiC/CobH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin biosynthesis precorrin-8X methylmutase CobH/CbiC" evidence="5">
        <location>
            <begin position="60"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB60091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="257" mass="28443" checksum="76CFDCD377E279BD" modified="2001-10-01" version="1">MLSVSVRNGSFIINKEYKINMDSDLFRKIAGDAKSINNSISSHILNSWAEHLKEWADPEKIEEESINIIETVTESIENARERYLFSKIVHAAGDFRLIDLIRMSPGFVDTVAEKLRDGCRIVVDAKMVKAGIYNKNLLDRCRIMYYGDDERVDRISKQMGITKSASAMRIASEDGVRDAIFVIGNAPTALIELLNIVRDTKEKPPAVIGVPVGFVQAARAKYSLVSSGIDYLTILGSRGGSPIAASVINAFGVMLDW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A63</accession>
    <name>CBID_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cobalt-precorrin-5B C(1)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.195</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobalt-precorrin-6A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiD</name>
        <name type="ordered locus">TV0947</name>
        <name type="ORF">TVG0972668</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:26285"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60063"/>
            <dbReference type="ChEBI" id="CHEBI:60064"/>
            <dbReference type="EC" id="2.1.1.195"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 6/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbiD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.195" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_052268470.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A63"/>
    <dbReference type="SMR" id="Q97A63"/>
    <dbReference type="STRING" id="273116.gene:9381739"/>
    <dbReference type="PaxDb" id="273116-14325164"/>
    <dbReference type="GeneID" id="1442026"/>
    <dbReference type="KEGG" id="tvo:TVG0972668"/>
    <dbReference type="eggNOG" id="arCOG04383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041273_1_0_2"/>
    <dbReference type="OrthoDB" id="57543at2157"/>
    <dbReference type="PhylomeDB" id="Q97A63"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043780">
        <property type="term" value="F:cobalt-precorrin-5B C1-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2110.10">
        <property type="entry name" value="CbiD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00787">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002748">
        <property type="entry name" value="CbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036074">
        <property type="entry name" value="CbiD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00312">
        <property type="entry name" value="cbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863">
        <property type="entry name" value="COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01888">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026782">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111342">
        <property type="entry name" value="CbiD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141702" description="Cobalt-precorrin-5B C(1)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00787"/>
        </source>
    </evidence>
    <sequence length="378" mass="39916" checksum="035CDD7A35275FE8" modified="2001-10-01" version="1">MMYASDGSGVNPFQQYGITTGLTAAAAAKACTLTVLKGVQNRVVVPTPIGIRIEVKVVESVRIDESSGYASAEKFSGDNPDQLNGITIRCHCKVVKKQENGRSKITISGNAGIGVVEKDGLGIRPGEKAISQGARKMIEDAVREAAGGYDVELSISVPNGEEFAKLTMNEKVGVFGGISVLGTTGIEEPVSTEEYELHLKYIVAAGRCVSKIIVLCPGNTALKFAKKYFALPDKAFVLIGDKVGAAVSASIESAYDHVVIFGLPGKLVKIAAGIYNTHSKVADGRMETLAAVAAMYGISKGAVKRIMESSNTGEAISIIEQEGIVAEVLNTIASRISNRLKADFSRSVGFSVVIIDHDGKIIGSHLDGHIKEVLKYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A87</accession>
    <name>Q97A87_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cas12f1-like TNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0952253</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917161.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A87"/>
    <dbReference type="SMR" id="Q97A87"/>
    <dbReference type="STRING" id="273116.gene:9381715"/>
    <dbReference type="PaxDb" id="273116-14325140"/>
    <dbReference type="DNASU" id="1442009"/>
    <dbReference type="GeneID" id="71201322"/>
    <dbReference type="KEGG" id="tvo:TVG0952253"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1965683_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97A87"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="26"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="127" mass="14424" checksum="457B5C8CC5B142D9" modified="2001-10-01" version="1">MGPAKKKKKGKEKKFRRELGSWSAVELEKFIEYKAEDAGKKVIYINPKYSPQKCSRCGYVSKENRHDSVFKCKNCGFELNADLNASRNIEVIRVSEYFRLLSASQSLLFNETPLTGGAGDQRQAPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AA7</accession>
    <name>Q97AA7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0923753 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0923753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917143.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AA7"/>
    <dbReference type="STRING" id="273116.gene:9381695"/>
    <dbReference type="PaxDb" id="273116-14325120"/>
    <dbReference type="DNASU" id="1441991"/>
    <dbReference type="GeneID" id="1441991"/>
    <dbReference type="KEGG" id="tvo:TVG0923753"/>
    <dbReference type="eggNOG" id="arCOG05382">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529309_0_0_2"/>
    <dbReference type="OrthoDB" id="55967at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058247">
        <property type="entry name" value="DUF1453"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07301">
        <property type="entry name" value="DUF1453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="175" mass="20260" checksum="77EE5C8579B74E96" modified="2001-10-01" version="1">MQLESLYQFLYSVVMSYLVSLKYVSFVLISVILVFRKIIRTVKGGRFRRYSIFIPPILYILFSIAVSYGTSLLGIVLSLIAFVFGIYISFHTTRSIVFFSKNNILYYKRPGWVVAIWSLAFLTRLALILFFPSFNGDLFTVLLFLASGLIIGESFWIVRKNREILSTVRMPKIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AB5</accession>
    <name>Q97AB5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0912906 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0912906</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AB5"/>
    <dbReference type="STRING" id="273116.gene:9381687"/>
    <dbReference type="PaxDb" id="273116-14325112"/>
    <dbReference type="KEGG" id="tvo:TVG0912906"/>
    <dbReference type="HOGENOM" id="CLU_2271191_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="102" mass="12017" checksum="5C20318E015F3682" modified="2001-10-01" version="1">MLYLPENKNSQVFSFMARLHTCIDKNEIEVIHGTLFYFYTDSYFNNKNHLCNKGKLMNVKLSPGDVIVIQTVDEFNHIGMEWRLMSDNCEILENTAYEVIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AC8</accession>
    <name>Q97AC8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rhamnosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0900412</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917127.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AC8"/>
    <dbReference type="STRING" id="273116.gene:9381674"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325099"/>
    <dbReference type="GeneID" id="1441975"/>
    <dbReference type="KEGG" id="tvo:TVG0900412"/>
    <dbReference type="eggNOG" id="arCOG01383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_917086_0_0_2"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="PhylomeDB" id="Q97AC8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02526">
        <property type="entry name" value="GT2_RfbF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43179:SF12">
        <property type="entry name" value="GALACTOFURANOSYLTRANSFERASE GLFT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43179">
        <property type="entry name" value="RHAMNOSYLTRANSFERASE WBBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="4">
        <location>
            <begin position="10"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="270" mass="31494" checksum="CBBCB5C0BAC5B82F" modified="2001-10-01" version="1">MNNDISIAALIVTYNPDKRLIESLTKLEKLVDFVVIVDNNSNKSPVLDYISNNIFNDRYLIQLEDNLGIAYALNTGIKYILEKGHYDYILTLDQDTVPLIDDIKELIKRANSIYSNIGIIALISKMDPKIPEFTDVKFPMTSGNIVKSEIFKYIKYREEFFIDSVDFDFDYNVRMHGYRIIQYNYRAIDHNVGITKNGFPYESSERLYYIIRNSTVLFGENKITFRMYIFQAFYWITMGLIHWEILKTAKSSVKGFKDGVNKKLGKNNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AD0</accession>
    <name>Q97AD0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0898533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AD0"/>
    <dbReference type="PaxDb" id="273116-14325097"/>
    <dbReference type="KEGG" id="tvo:TVG0898533"/>
    <dbReference type="eggNOG" id="arCOG10339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1902055_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="133" mass="15384" checksum="E9641E2D0419A6B0" modified="2001-10-01" version="1">MHHSLLISMDVDSISMEAQRAIDTLRKSSLAEPVIQSDNGSSFIAMEFKHVLRENHLTQKLIRPHTQENNAIVERANKTMRESWYLSYRLITNRRNPKSPVSFSTTITKEDIPHCSISHRYSITGAILKYSWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AE0</accession>
    <name>Q97AE0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase beta</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
            <ecNumber evidence="4">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">5'-deoxyribose-phosphate lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">AP lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">TVG0888403</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="24 25" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 27">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 5'-end 2'-deoxyribose-2'-deoxyribonucleotide-DNA = (2E,4S)-4-hydroxypenten-2-al-5-phosphate + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:76255"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:18657"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:195194"/>
            <dbReference type="ChEBI" id="CHEBI:195195"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EC" id="4.2.99.18" evidence="4"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917114.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AE0"/>
    <dbReference type="STRING" id="273116.gene:9381662"/>
    <dbReference type="PaxDb" id="273116-14325087"/>
    <dbReference type="DNASU" id="1441962"/>
    <dbReference type="GeneID" id="1441962"/>
    <dbReference type="KEGG" id="tvo:TVG0888403"/>
    <dbReference type="eggNOG" id="arCOG00305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017729_1_0_2"/>
    <dbReference type="OrthoDB" id="8999at2157"/>
    <dbReference type="PhylomeDB" id="Q97AE0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00141">
        <property type="entry name" value="NT_POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.210.10">
        <property type="entry name" value="DNA polymerase, thumb domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002054">
        <property type="entry name" value="DNA-dir_DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037160">
        <property type="entry name" value="DNA_Pol_thumb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022312">
        <property type="entry name" value="DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002008">
        <property type="entry name" value="DNA_pol_X_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276:SF28">
        <property type="entry name" value="DNA POLYMERASE LAMBDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276">
        <property type="entry name" value="DNA POLYMERASE TYPE-X FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00870">
        <property type="entry name" value="DNAPOLXBETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00483">
        <property type="entry name" value="POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="13" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase X" evidence="23">
        <location>
            <begin position="1"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="22">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="22">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="22">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="BAB60012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="516" mass="57042" checksum="506254FD31145961" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AF3</accession>
    <name>Q97AF3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0878378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917101.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AF3"/>
    <dbReference type="STRING" id="273116.gene:9381649"/>
    <dbReference type="PaxDb" id="273116-14325074"/>
    <dbReference type="GeneID" id="1441949"/>
    <dbReference type="KEGG" id="tvo:TVG0878378"/>
    <dbReference type="eggNOG" id="arCOG02759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101320_2_0_2"/>
    <dbReference type="OrthoDB" id="92826at2157"/>
    <dbReference type="PhylomeDB" id="Q97AF3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1290">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033573">
        <property type="entry name" value="transpos_IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360:SF2">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01321">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS200-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="134" mass="15868" checksum="B7D0AE78B9B7B3A4" modified="2001-10-01" version="1">MELKSTRHVKYLCNYHYLWIPKYRRAILTNEVAEYTKEVLKSIAKELGCEIIALEVMPDHIHLFVNCPPRYSPSYLTNYFKGKSARLILKKSPELRRYKKLWSRSYFVSTAGNVSSETIKKYIEEQWVKEGEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AF8</accession>
    <name>Q97AF8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cofactor biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG0874800</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760250.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AF8"/>
    <dbReference type="STRING" id="273116.gene:9381644"/>
    <dbReference type="PaxDb" id="273116-14325069"/>
    <dbReference type="DNASU" id="1441944"/>
    <dbReference type="GeneID" id="1441944"/>
    <dbReference type="KEGG" id="tvo:TVG0874800"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AF8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21123">
        <property type="entry name" value="SPASM_MftC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04053">
        <property type="entry name" value="TIGR04053 family radical SAM/SPASM domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF34">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="7"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB59994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="370" mass="41519" checksum="7767440269C32A9C" modified="2001-10-01" version="1">MMGYNFNEKPLLIFWETTKACGLKCEHCRASAILEALPDEMNYDESIDFLSHIKEFGKPYPIVILTGGDMLRKRRINDIMKYLKDAEIPFSVSPAVTEMLTDERIKSFLEFGVSSVSISLDGMKDVHEKVRGVPGVFDDTVRVVEKLIKEGIPMQINTVVMRSTVNDLPKVLKLILDKSVKVWEVFFLIKTGRGIDREDLSPEEYEDVNKYLLYATGYGIRIRTVESPIFRRITLQSGSTGRIEGGPLYQKLVSETEKIIGKKPSAIAKSVIPTRDGYGIIFVGHNGDVYPSGFLPYKVGNVKNQSIVDIYRKSELLRKIRDPDNLVGKCGFCEYRSVCGGSRARAFAYSSDPFGTDPSCSYMPKSVVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AF9</accession>
    <name>Q97AF9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0872883</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917095.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AF9"/>
    <dbReference type="STRING" id="273116.gene:9381643"/>
    <dbReference type="PaxDb" id="273116-14325068"/>
    <dbReference type="DNASU" id="1441943"/>
    <dbReference type="GeneID" id="1441943"/>
    <dbReference type="KEGG" id="tvo:TVG0872883"/>
    <dbReference type="eggNOG" id="arCOG02702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_16_1_2"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="PhylomeDB" id="Q97AF9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="38"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="185" mass="21090" checksum="DE3E61816263C6C8" modified="2001-10-01" version="1">MHFGKHNVDFMLPSLRKEWEDPEKIVSQIPIQPNFDIVDLGCGPGFFTLPLSETTDGKVYAIDASQEMIDALKQRIDGRSNIVTIKAPAEKIPLPDLSADLVFIANAYHDFDDRDEVLSEIYRILRAGRFLVDIDWKKEETTRGPPIDIRIDKKEAMLEIASHNFDIESEIEAGPHHYGLIFKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AG4</accession>
    <name>Q97AG4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoglycerate mutase (2,3-diphosphoglycerate-dependent)</fullName>
            <ecNumber evidence="2">5.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0869690</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AG4"/>
    <dbReference type="STRING" id="273116.gene:9381638"/>
    <dbReference type="PaxDb" id="273116-14325063"/>
    <dbReference type="KEGG" id="tvo:TVG0869690"/>
    <dbReference type="eggNOG" id="arCOG01991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_8_4_2"/>
    <dbReference type="PhylomeDB" id="Q97AG4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="5">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="26"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="206" mass="23312" checksum="5D1235F3714A8331" modified="2001-10-01" version="1">MGISTMHKAEDLRRSSDIMKKVYLVRHAKTIMNYTNRWQGRSDSDILPGAIEKLLSSIKSIENEGINKIYSSCIGRAIKSAAAAANVLGLNSIFLDCRFNERDLGLLEGKTVDEIRTKFGIEFLYITSRDIEKLPYVESWPDFVSRVFSGLESLPDGRTLLITHGGVLRAVYNTLSHSDERRVIFDNGDILVLNKTDSWDIERIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AH0</accession>
    <name>Q97AH0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-hydroxyacyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0862758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917084.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AH0"/>
    <dbReference type="STRING" id="273116.gene:9381632"/>
    <dbReference type="PaxDb" id="273116-14325057"/>
    <dbReference type="GeneID" id="1441932"/>
    <dbReference type="KEGG" id="tvo:TVG0862758"/>
    <dbReference type="eggNOG" id="arCOG00250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_2"/>
    <dbReference type="OrthoDB" id="51300at2157"/>
    <dbReference type="PhylomeDB" id="Q97AH0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022694">
        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004699">
        <property type="entry name" value="PRK06035.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000105">
        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="4">
        <location>
            <begin position="5"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="3">
        <location>
            <begin position="189"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="294" mass="32377" checksum="6BEC32E9C456B738" modified="2001-10-01" version="1">MEISNIGVVGSGVMGQGIAQVFAKAGYKVTIVDVNEQILENALKAISDGKYGLRNLVNKGQISENDLNSIMSRISTSTEYTSLSSCDFIVEAVPEDLRIKREVFAKLESNVSKDTILASNTSGIMIAEITSGLSYKERIIGMHWFNPAPVMKLIEVVKAQLTSEETFNTTMELAKEIGKVPIKVADVPGFYTTRFIEGWLLSAIRSAEQGVATRKDIDAMTKMAFGFPMGPFELMDIIGIDTVYHIATYLYEETGDPQFIPPISLKQMVLNGYIGDKKVKFGSKGGWFDLYGHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AI5</accession>
    <name>Q97AI5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-methylhydantoinase ATP-hydrolyzing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0834091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917069.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AI5"/>
    <dbReference type="PaxDb" id="273116-14325041"/>
    <dbReference type="GeneID" id="1441917"/>
    <dbReference type="KEGG" id="tvo:TVG0834091"/>
    <dbReference type="eggNOG" id="arCOG01512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020413_0_0_2"/>
    <dbReference type="OrthoDB" id="8261at2157"/>
    <dbReference type="PhylomeDB" id="Q97AI5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="533" mass="57745" checksum="3E9AF5FE0B5336AF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AJ7</accession>
    <name>Q97AJ7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Maturation protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0815451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AJ7"/>
    <dbReference type="STRING" id="273116.gene:9381603"/>
    <dbReference type="PaxDb" id="273116-14325029"/>
    <dbReference type="KEGG" id="tvo:TVG0815451"/>
    <dbReference type="eggNOG" id="arCOG00322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_4_1_2"/>
    <dbReference type="PhylomeDB" id="Q97AJ7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43666:SF1">
        <property type="entry name" value="CONSERVED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43666">
        <property type="entry name" value="TLDD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="1">
        <location>
            <begin position="209"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="431" mass="48446" checksum="17928F02275132F1" modified="2001-10-01" version="1">MIEMADVEHLIRKLSDNFEEYVVKQEYASDTQVRFSNSKLDLTNIWDEYGIDIFVSSGRKTASIAVHSPDEGISKIDYLQKVLSNSPENSIYEGINDKKQIYRAVDYKDDEKDVQDMAVAAISSAEDEGARRVSGLIYKRSSEESIFTRYNTYSAHKYGIEAAVRSFIGESSGQYTAHYGPSSKADTEEVGKRSVRSILKVDSVKGNEGKYRVIFSPMAFGNIISYGSYLFSAYSIISGMSYLEGRIGKEVANESFSLVDDPTDTKGIGFEYFDDEATATKRNTIIERGILKTYLHSYSTAKKMHTETTGNAGIISPEAWQLEVDGGDSSLDEMIADTKEGVVIMNSWYLRFQDYRNGVFSTVPRDGIYHIVKGEIKESWSGIRISDSYSNVLSNISLISKEAEYVKWWNEISASKMPYIAIDKVTLTRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AK0</accession>
    <name>Q97AK0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine hydroxymethyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0811706</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AK0"/>
    <dbReference type="STRING" id="273116.gene:9381600"/>
    <dbReference type="PaxDb" id="273116-14325026"/>
    <dbReference type="KEGG" id="tvo:TVG0811706"/>
    <dbReference type="eggNOG" id="arCOG00070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_2"/>
    <dbReference type="PhylomeDB" id="Q97AK0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046653">
        <property type="term" value="P:tetrahydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="3">
        <location>
            <begin position="5"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="389" mass="43600" checksum="F63C72A98EE562B0" modified="2001-10-01" version="1">MAMRKILSIVQDADIYRSSVINLQASENVISPNVRRALASDFASRYSHKENGVNDYGGTKYAEELEESVNELASEVFNFKYADAKPLSGHVAAETVLAALVKRGESIMKIPERNGGYQGYLNGYLPSLMGFRSYDIPMKPDQAIDFESFEKTIDYIRPKVVILGQSVFVKSYDIPKVRELCNSVGSMLLYDASHVMGLIAGGTFQKDIGLCDVVFGSTHKTFFGPQGGIILTNEAEVFDRIEKKITWITMDNFNISRMAGVGVALEETKKFGREYASRVVKNAKDLAKELDGKLPIKYSPWFTESHQVLIDEEGLKKDGYDTIKFSEDLEKNGIIVDRIGRIGTAEITRMGVDDVQPIADMIKDAYIGKDVKERVNKFVSNMKMRYYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AL7</accession>
    <name>Q97AL7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0794454 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0794454</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917037.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AL7"/>
    <dbReference type="STRING" id="273116.gene:9381583"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="242" mass="27689" checksum="FE19FC621B7B3ED9" modified="2001-10-01" version="1">MDDVETLIKNADRNFEKEKYIDALRDYLAAIEKVQDDDLKAEIAYKVSQIYHYLQKDSDENSMKFAEIALKIHEEKGEMDLQVLDLINIGTILIDSGKKEDGMRKLDEAIEKAKETNDDELIMISLTSKAGIISDSDPDEAFKIYQDVAEKSEKIEDWEDYFDALSGLINITRKKDEGKAFQMAMDAIDKLEKISATIKSKKERKEFIESFSYLYDTASDIAMALDNVEDAIEIAKRLQNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AM4</accession>
    <name>Q97AM4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methylmalonyl-CoA mutase</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">TVG0789597</name>
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    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917030.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AM4"/>
    <dbReference type="STRING" id="273116.gene:9381576"/>
    <dbReference type="PaxDb" id="273116-14325002"/>
    <dbReference type="GeneID" id="1441878"/>
    <dbReference type="KEGG" id="tvo:TVG0789597"/>
    <dbReference type="eggNOG" id="arCOG04232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009523_5_1_2"/>
    <dbReference type="OrthoDB" id="38408at2157"/>
    <dbReference type="PhylomeDB" id="Q97AM4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF1">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cobalamin (vitamin B12)-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="45"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="565" mass="64988" checksum="E673336F114191FF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AM5</accession>
    <name>Q97AM5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0788047 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0788047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AM5"/>
    <dbReference type="PaxDb" id="273116-14325001"/>
    <dbReference type="KEGG" id="tvo:TVG0788047"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="75" mass="8761" checksum="EC6EAEF9607BADF0" modified="2001-10-01" version="1">MNVAKRYFISMPYISISLIYNVLLGKIWIAIGEKSLMRSPSQKRTMNMSAGSQILWILGIKKLDIKKEYIRILQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AM8</accession>
    <name>Q97AM8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-terminal acetyltransferase complex subunit [ARD1]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0785733</name>
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    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="273116.gene:9381572"/>
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    <dbReference type="KEGG" id="tvo:TVG0785733"/>
    <dbReference type="eggNOG" id="arCOG00826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_36_1_2"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052829">
        <property type="entry name" value="N-acetyltransferase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43259:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43259">
        <property type="entry name" value="SPT10P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="148" mass="17507" checksum="172761D1F0A038D9" modified="2001-10-01" version="1">MKKEDMESVMDQLLRLKRLNSEFDCIFNVSEQSKADIEKYINKIMNSDDHVLLVAELDNKITGILFADILFRIYYEPKYEARIREFYVMPEYRRNGIGKKLIDALKRELDPKGITFITAEFPALNLIAQNFYKKIGYREIVTIYGQIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AS1</accession>
    <name>Q97AS1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0742218 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0742218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AS1"/>
    <dbReference type="STRING" id="273116.gene:9381528"/>
    <dbReference type="PaxDb" id="273116-14324954"/>
    <dbReference type="KEGG" id="tvo:TVG0742218"/>
    <dbReference type="HOGENOM" id="CLU_2476212_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="87" mass="10121" checksum="B25DEA967422B367" modified="2001-10-01" version="1">MDLILLQYLCHCIKRANDMEKDKANIEKCIYYNSGNCELSSYVTKCTLCLNYKSFYQIGKKKKLIYDIFDLEPEIAEISSGSQSKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AT9</accession>
    <name>Q97AT9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0727696 protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760319.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
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    <dbReference type="SMR" id="Q97AT9"/>
    <dbReference type="REBASE" id="685111">
        <property type="entry name" value="M.TvoIII"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324936"/>
    <dbReference type="GeneID" id="1441826"/>
    <dbReference type="KEGG" id="tvo:TVG0727696"/>
    <dbReference type="eggNOG" id="arCOG07392">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_623518_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA methylase adenine-specific" evidence="1">
        <location>
            <begin position="102"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="439" mass="51711" checksum="338815867BA40240" modified="2001-10-01" version="1">MHHTEKSILMKRRTLGEHLTSVNIFKEYILPKIKDKINCYTWVDLFAGEGNLILPILDLVPEEKRIEFFRDNIFLFDIQKEMVERAIKNAESYRIPRMIAEKNIQVKDTLKEYPLLNVKKPIYHITNPPYLYIGYIAKHKENFGQLDYFTASLKGLQDLYQIALMNDLQNEINQMVYIIPSNFLFGHSVSNLIRRKFLPWYKINDAIIFEKKIFENTGVNVAICFFERTTAKNSTIKFKATKINGEIKEREYILSHKNDYRAGTEFDDYIKSQEKNTLKISYYLTKKEIDNNPGNNKVLLLNSKEYKNGEYIKESFFVNDKLYEKIIRNPLFIRTVDTGNENGRAGLYYIKDVFGTDGIFVEGNTYRTNPIQVFIEPYLSLKQLKQLQLSFNQKLNELRAITDSEFMTTYKYSNNAKYTRKYLGLSQAKQLLEVIDIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AW7</accession>
    <name>Q97AW7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0699210 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0699210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916952.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AW7"/>
    <dbReference type="STRING" id="273116.gene:9381480"/>
    <dbReference type="PaxDb" id="273116-14324908"/>
    <dbReference type="GeneID" id="1441800"/>
    <dbReference type="KEGG" id="tvo:TVG0699210"/>
    <dbReference type="eggNOG" id="arCOG06970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032984_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="121" mass="13421" checksum="572FB8A3CF20EBAE" modified="2001-10-01" version="1">MGAEEYYYNKFLEWQSKGNGDYRVYLRRVRQPGLAVEFRQDPNFRTEICSYLKAYAQGQEKDTIMSVIKGITNPDADILEIIIGATLDACYPNSNIGNALVGLAIAGLIGAALIALLSNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AX2</accession>
    <name>Q97AX2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TVG0696003 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0696003</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916948.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AX2"/>
    <dbReference type="STRING" id="273116.gene:9381475"/>
    <dbReference type="CAZy" id="GH5">
        <property type="family name" value="Glycoside Hydrolase Family 5"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324903"/>
    <dbReference type="GeneID" id="1441796"/>
    <dbReference type="KEGG" id="tvo:TVG0696003"/>
    <dbReference type="eggNOG" id="arCOG05411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_429428_0_0_2"/>
    <dbReference type="OrthoDB" id="92642at2157"/>
    <dbReference type="PhylomeDB" id="Q97AX2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="3">
        <location>
            <begin position="31"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="647" mass="74167" checksum="B5D880C49056B06E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AX8</accession>
    <name>Q97AX8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="2">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0684360</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AX8"/>
    <dbReference type="STRING" id="273116.gene:9381469"/>
    <dbReference type="REBASE" id="685112">
        <property type="entry name" value="TvoORF4360P"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324897"/>
    <dbReference type="KEGG" id="tvo:TVG0684360"/>
    <dbReference type="eggNOG" id="arCOG02635">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002539_1_1_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006304">
        <property type="term" value="P:DNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011639">
        <property type="entry name" value="MethylTrfase_TaqI-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050953">
        <property type="entry name" value="N4_N6_ade-DNA_methylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33841:SF5">
        <property type="entry name" value="DNA METHYLASE (MODIFICATION METHYLASE) (METHYLTRANSFERASE)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33841">
        <property type="entry name" value="DNA METHYLTRANSFERASE YEEA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07669">
        <property type="entry name" value="Eco57I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Type II methyltransferase M.TaqI-like" evidence="8">
        <location>
            <begin position="405"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="461"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB59823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="985" mass="115410" checksum="07EE690CAB7370B0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AZ0</accession>
    <name>Q97AZ0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">TVG0664080</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AZ0"/>
    <dbReference type="STRING" id="273116.gene:9381457"/>
    <dbReference type="PaxDb" id="273116-14324885"/>
    <dbReference type="KEGG" id="tvo:TVG0664080"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_1_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="PhylomeDB" id="Q97AZ0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="124"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="233"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="420" mass="46980" checksum="4E5783687980AEBD" modified="2001-10-01" version="1">MEDMLLNEEEKQIRDLCRRVVKEVPHELIRKIEREEVTFPREFIDFITKNGVVGLRFPKEYGGKGASWVAEASAVEEMGYLGFTLSCMYSLGTIVGEPMYRYGTNEQKEKYLKGITSGKLYGAEAITEPSGGSDLFGMMKTTAVKKGNKYILNGQKRFIVGGKGADFFVTYAITDPNAKSRTRGISAFIVDRDTPGLKVETLYGLMGNKGGGTARIVYKDAEVPEENLIGELNGGYEVFNRMMIPERLTTAAGSLGVSTAALKVATDYALKREAFGSKLIEHEGINFKLAESLTLLQSASALVYTAAKAADLYEKGQTTQSYARKLVSMAKLHSTEAMWKIVNDAMQIMGGIGYTTVYPIERLLRDSRLGLIWTGSSEVMKMIIQHEYLKEFTNPDYWESRRDIEKDALDFRLEEEKVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B12</accession>
    <name>Q97B12_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0642496 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0642496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916906.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B12"/>
    <dbReference type="STRING" id="273116.gene:9381435"/>
    <dbReference type="PaxDb" id="273116-14324863"/>
    <dbReference type="GeneID" id="1441754"/>
    <dbReference type="KEGG" id="tvo:TVG0642496"/>
    <dbReference type="eggNOG" id="arCOG07386">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922919_0_0_2"/>
    <dbReference type="OrthoDB" id="378980at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="131" mass="15247" checksum="246611C2A1A5623E" modified="2001-10-01" version="1">MGRLESESLKKFIAEHSKILETDNLNDIIDRLDNGTFYIDPSQIASDGLYSFISEIKDILAEREILKDNRTIKYMKIGLSFEMTLTLWNEEICYYRSMIKVGKKIKFVNCRVNFTRYGVEVSLGDRGFIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="139" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B15</accession>
    <name>Q97B15_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG0641301</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916903.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="STRING" id="273116.gene:9381432"/>
    <dbReference type="PaxDb" id="273116-14324860"/>
    <dbReference type="GeneID" id="1441751"/>
    <dbReference type="KEGG" id="tvo:TVG0641301"/>
    <dbReference type="eggNOG" id="arCOG00557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002872_0_0_2"/>
    <dbReference type="OrthoDB" id="372104at2157"/>
    <dbReference type="PhylomeDB" id="Q97B15"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17922">
        <property type="entry name" value="DEXHc_LHR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18796">
        <property type="entry name" value="SF2_C_LHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="Lhr-like_DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010338">
        <property type="entry name" value="PRK13767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF6">
        <property type="entry name" value="LARGE HELICASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="WHD_Lhr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="40"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="276"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1713" mass="197506" checksum="45D0CC27F4618842" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B58</accession>
    <name>UPPS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="1">2.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="1">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="1">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uppS</name>
        <name type="ordered locus">TV0600</name>
        <name type="ORF">TVG0591988</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>geranylgeranyl diphosphate + 7 isopentenyl diphosphate = tri-trans,hepta-cis-undecaprenyl diphosphate + 7 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27622"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:60388"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.89" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916858.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B58"/>
    <dbReference type="SMR" id="Q97B58"/>
    <dbReference type="STRING" id="273116.gene:9381388"/>
    <dbReference type="PaxDb" id="273116-14324815"/>
    <dbReference type="GeneID" id="1441706"/>
    <dbReference type="KEGG" id="tvo:TVG0591988"/>
    <dbReference type="eggNOG" id="arCOG01532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_2_0_2"/>
    <dbReference type="OrthoDB" id="8293at2157"/>
    <dbReference type="PhylomeDB" id="Q97B58"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000003">
        <property type="entry name" value="Isoprenyl transferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000123745" description="Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="82"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="213"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <sequence length="258" mass="30220" checksum="46B1160A8B9D5E67" modified="2001-10-01" version="1">MGISGKLGDIASKVYEDVLMEEVKKYPRPRHVGIITDGNRRYARIVGIPENEGHVKGKNKVEEVLDWCMELDIKIVTFYAFSTENFKRSPEEVDFLFHLINDAFISLLKDERVYKNKINVKVIGNVSMLPEYLRETIKITEETTKNFSNYHMNLAIGYGGREEILDAIKRIARDAIAGKINVDEIDESKFRNYLYDGNLPDPDLILRTSGEERISNFLLWQSAYSELYFSDVYWPEFSKLDFLRAIYSYQRRQRRFGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B59</accession>
    <name>TIAS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS</fullName>
            <shortName evidence="1">tRNA(Ile2)-agm2C synthetase</shortName>
            <ecNumber evidence="1">6.3.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA(Ile2) agmatidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tiaS</name>
        <name type="ordered locus">TV0599</name>
        <name type="ORF">TVG0590669</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(34) in tRNA(Ile2) + agmatine + ATP + H2O = 2-agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43608"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10626"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83545"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TiaS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.22" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916857.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B59"/>
    <dbReference type="SMR" id="Q97B59"/>
    <dbReference type="STRING" id="273116.gene:9381387"/>
    <dbReference type="PaxDb" id="273116-14324814"/>
    <dbReference type="GeneID" id="1441705"/>
    <dbReference type="KEGG" id="tvo:TVG0590669"/>
    <dbReference type="eggNOG" id="arCOG01115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_675459_0_0_2"/>
    <dbReference type="OrthoDB" id="39189at2157"/>
    <dbReference type="PhylomeDB" id="Q97B59"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04482">
        <property type="entry name" value="RPA2_OBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01892">
        <property type="entry name" value="tRNA_Ile2_agm2C_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053870">
        <property type="entry name" value="TiaS-like_TCKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="TiaS_FLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024913">
        <property type="entry name" value="tRNA_Ile2__agm2C_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055394">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705:SF2">
        <property type="entry name" value="DUF1743 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="TiaS_FLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22641">
        <property type="entry name" value="TiaS_TCKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23783">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000407306" description="tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS">
        <location>
            <begin position="1"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01892"/>
        </source>
    </evidence>
    <sequence length="437" mass="49674" checksum="FB358153AB562755" modified="2001-10-01" version="1">MFLAFDDTDSPSGMCTTYLMEEFLRKVNLDVIGYPRLVRLNPNIRYKTRGNGALSVHLGRGIGKKHTIGELHGKILYGYAEGEDEYDENVLYVMKDLVEKYSELDYFNTNPGIVVSKNPFPENYYWSALEREIRIEEAENFITENNGKFLKFKSGRGIIGSGAAISWPATRTTYEILAYKYPHPEEIETEKKMRLSILADTFRGTFNNVDIANKYPAIFPNPKTPVIFGIRGLYPSVLANAAKKVIDDGSINYDSTVTYLTNQATDDHIIDEPNVIEDLHSYKITAEIIDKPFSVAGGHYFVRSISRAGEFTAAAFEPTKEFRHTFSKLMPGDTVTFYGSFTNGNLNVEKMQIISVSRVFSRVTPLCKFCNTRTKSKGKNDFRCPKCGRRYNTPDYHEVKREISPGKYDVPVVARRHLSMPFEIESMFKTKINALEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-09-27" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B60</accession>
    <name>GMHB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Probable D-glycero-D-manno-heptose-1,7-bisphosphate 7-phosphatase</fullName>
            <ecNumber>3.1.3.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>D,D-heptose 1,7-bisphosphate phosphatase</fullName>
            <shortName>HBP phosphatase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">gmhB</name>
        <name type="ordered locus">TV0598</name>
        <name type="ORF">TVG0590688</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts the D-glycero-D-manno-heptose 1,7-bisphosphate intermediate into D-glycero-D-manno-heptose 1-phosphate by removing the phosphate group at the C-7 position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>D-glycero-D-manno-heptose 1,7-bisphosphate + H2O = D-glycero-D-manno-heptose 1-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:48504"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59957"/>
            <dbReference type="ChEBI" id="CHEBI:60002"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GmhB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B60"/>
    <dbReference type="SMR" id="Q97B60"/>
    <dbReference type="STRING" id="273116.gene:9381386"/>
    <dbReference type="PaxDb" id="273116-14324813"/>
    <dbReference type="KEGG" id="tvo:TVG0590688"/>
    <dbReference type="eggNOG" id="arCOG07384">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085077_4_1_2"/>
    <dbReference type="PhylomeDB" id="Q97B60"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034200">
        <property type="term" value="F:D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07503">
        <property type="entry name" value="HAD_HisB-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004446">
        <property type="entry name" value="Heptose_bisP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006543">
        <property type="entry name" value="Histidinol-phos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01656">
        <property type="entry name" value="Histidinol-ppas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42891">
        <property type="entry name" value="D-GLYCERO-BETA-D-MANNO-HEPTOSE-1,7-BISPHOSPHATE 7-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42891:SF1">
        <property type="entry name" value="D-GLYCERO-BETA-D-MANNO-HEPTOSE-1,7-BISPHOSPHATE 7-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08645">
        <property type="entry name" value="PNK3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004682">
        <property type="entry name" value="GmhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000209411" description="Probable D-glycero-D-manno-heptose-1,7-bisphosphate 7-phosphatase">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="49"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="99"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Stabilizes the phosphoryl group" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to substrate recognition" evidence="1">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes the phosphoryl group" evidence="1">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q7WG29"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="146" mass="16939" checksum="8A10D3962F54D74F" modified="2001-10-01" version="1">MKTLFIDRDGTINKDCPYCHNPSDLFIYPDAVDLLQRYQDDGFRIIIVTNQSGIGRGYFTESQFKEFSDFMNDKLKENGIVISAIYYCPHKPEDSCNCRKPETGLFSEVLEDYKVDIPSSIVVGDRNEIDGEFARRIKLPFRLLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B66</accession>
    <name>Q97B66_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Iron-sulfur protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0580405</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916850.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B66"/>
    <dbReference type="STRING" id="273116.gene:9381380"/>
    <dbReference type="PaxDb" id="273116-14324807"/>
    <dbReference type="DNASU" id="1441698"/>
    <dbReference type="GeneID" id="1441698"/>
    <dbReference type="KEGG" id="tvo:TVG0580405"/>
    <dbReference type="eggNOG" id="arCOG00335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027059_3_0_2"/>
    <dbReference type="PhylomeDB" id="Q97B66"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004452">
        <property type="entry name" value="LutB/LldF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153:SF2">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="289"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="381" mass="42356" checksum="2F2FC47BCAA3DDB5" modified="2001-10-01" version="1">MNWSQIISLVSAGNYMEVEKILQDYPYIVDMADHLREQKLKVIANLDTYVKRTMDSVEKLGGHAYLAKDKDEARKIIGDLVGEKKTVVFSKTNVAYEIGLREFLQERNNEVWETDLGEYLVQISRGWPTHIVAPALDMTREEAAKAVKKIDPSINENSKIEDIVAAVRKFLMSKYVKADVGITGANAVAADSGSVALVENEGNIRIDTVMPPMHIAVTGIDKIVPTMRDALDEVMVQAAYAGIFPPTYINVTSGPSSTADIELRRVSPATGPKDFHLVLIDNGRLEASKNEDLKEALLCIKCGRCYFSCPIYRTLGKEWISTKSPYNGPTGVMWNYITNKDPWPASYCVHSGGCKEVCPMKINIPEVIKYIKFIGSKQVGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BC1</accession>
    <name>RL15E_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Large ribosomal subunit protein eL15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L15e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl15e</name>
        <name type="ordered locus">TV0535</name>
        <name type="ORF">TVG0525551</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916793.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BC1"/>
    <dbReference type="SMR" id="Q97BC1"/>
    <dbReference type="STRING" id="273116.gene:9381319"/>
    <dbReference type="PaxDb" id="273116-14324750"/>
    <dbReference type="GeneID" id="1441051"/>
    <dbReference type="KEGG" id="tvo:TVG0525551"/>
    <dbReference type="eggNOG" id="arCOG04209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080796_1_0_2"/>
    <dbReference type="OrthoDB" id="8183at2157"/>
    <dbReference type="PhylomeDB" id="Q97BC1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000002">
        <property type="entry name" value="50S ribosomal protein L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00256">
        <property type="entry name" value="Ribosomal_eL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020926">
        <property type="entry name" value="Ribosomal_eL15_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000127589" description="Large ribosomal subunit protein eL15">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="197" mass="22974" checksum="35AE757BE86A4306" modified="2001-10-01" version="1">MSFTNLYQEIREEWKSLKKSEIYDVEKSRMIGWRHGPSVVRLDHPTRIDRARALGYKSKDGFIVVRSRVRRGGSNREKIMGGRRPRRLAYNKLTRKKSLKLIAEERAADKYPNLEVLNSYYVGEDGLYKYYEVILVDKSHPNIYNDPHISWISEPQNTGRVYRGLTSAGYKVRGLRTGRKGSSKSRPSIRANGRLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BC9</accession>
    <name>Q97BC9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0517863</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916785.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BC9"/>
    <dbReference type="STRING" id="273116.gene:9381311"/>
    <dbReference type="PaxDb" id="273116-14324742"/>
    <dbReference type="GeneID" id="1441043"/>
    <dbReference type="KEGG" id="tvo:TVG0517863"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="PhylomeDB" id="Q97BC9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="279" mass="30528" checksum="4107D5D7194A2D1A" modified="2001-10-01" version="1">MIETRSLTKVYKNGNNALKDISLDIGSGITSVIGRNGAGKTTMMRIFSTQLRPTSGSVTINGYDVVKESNKVRKIICSIPQEASPIGVLTPLEQVKMYLVARGLSISSADREARIALSSVGLEESMKAPADTLSGGMKRKVFVAMALSANADIVFLDEPTTGLDPMSRLEVWSLIRNLRSDIVLTTHYMEEAASLSKYIIMVDHGTVIKEGTLKELLSEFDGLIRVESRQPLPDSSPVGGIYIKYVNASEANEYISMGCDVKNITLDDVFIKERIYLEP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-01-17" modified="2026-01-28" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BF6</accession>
    <name>KDPB_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Potassium-transporting ATPase ATP-binding subunit</fullName>
            <ecNumber evidence="1">7.2.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP phosphohydrolase [potassium-transporting] B chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Potassium-binding and translocating subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Potassium-translocating ATPase B chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kdpB</name>
        <name type="ordered locus">TV0500</name>
        <name type="ORF">TVG0489285</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit is responsible for energy coupling to the transport system and for the release of the potassium ions to the cytoplasm.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>K(+)(out) + ATP + H2O = K(+)(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:16778"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The system is composed of three essential subunits: KdpA, KdpB and KdpC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.6" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916758.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BF6"/>
    <dbReference type="SMR" id="Q97BF6"/>
    <dbReference type="STRING" id="273116.gene:9381283"/>
    <dbReference type="PaxDb" id="273116-14324715"/>
    <dbReference type="GeneID" id="1441016"/>
    <dbReference type="KEGG" id="tvo:TVG0489285"/>
    <dbReference type="eggNOG" id="arCOG01577">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025728_2_0_2"/>
    <dbReference type="OrthoDB" id="8588at2157"/>
    <dbReference type="PhylomeDB" id="Q97BF6"/>
    <dbReference type="BRENDA" id="7.2.2.6">
        <property type="organism ID" value="6326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008556">
        <property type="term" value="F:P-type potassium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00285">
        <property type="entry name" value="KdpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006391">
        <property type="entry name" value="P-type_ATPase_bsu_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01497">
        <property type="entry name" value="kdpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43743">
        <property type="entry name" value="POTASSIUM-TRANSPORTING ATPASE ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43743:SF1">
        <property type="entry name" value="POTASSIUM-TRANSPORTING ATPASE ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-0633">Potassium transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000046153" description="Potassium-transporting ATPase ATP-binding subunit">
        <location>
            <begin position="1"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="599"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="644"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="1">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="367"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00285"/>
        </source>
    </evidence>
    <sequence length="668" mass="73105" checksum="270E33FC5C339F00" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-09-27" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BG7</accession>
    <name>GRPE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein GrpE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">HSP-70 cofactor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">grpE</name>
        <name type="ordered locus">TV0488</name>
        <name type="ORF">TVG0473313</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916747.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BG7"/>
    <dbReference type="SMR" id="Q97BG7"/>
    <dbReference type="STRING" id="273116.gene:9381270"/>
    <dbReference type="PaxDb" id="273116-14324703"/>
    <dbReference type="GeneID" id="1441005"/>
    <dbReference type="KEGG" id="tvo:TVG0473313"/>
    <dbReference type="eggNOG" id="arCOG04772">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057217_5_2_2"/>
    <dbReference type="OrthoDB" id="56041at2157"/>
    <dbReference type="PhylomeDB" id="Q97BG7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF23">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000113913" description="Protein GrpE">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01151"/>
        </source>
    </evidence>
    <sequence length="176" mass="20243" checksum="F82EFA4825ADF4B8" modified="2001-10-01" version="1">MYSPSSSNYIKDPISTEIIKSKTRNLKKRAEEAILYRSIAEQSSRKLAEISEAYKHKLADMENYLKIKDRETEIIRKNANESLIKDFLPVIDSMDAAIQAEKDNNLIRIRDQMLSILSKYGLQPIKAEGEKFDPYLHEAIGMTQDGEDGKIKYEVQRGYTLNNSVLRTSKVIVVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BI8</accession>
    <name>Q97BI8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0456214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BI8"/>
    <dbReference type="STRING" id="273116.gene:9381249"/>
    <dbReference type="PaxDb" id="273116-14324682"/>
    <dbReference type="KEGG" id="tvo:TVG0456214"/>
    <dbReference type="eggNOG" id="arCOG02759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101320_2_0_2"/>
    <dbReference type="OrthoDB" id="92826at2157"/>
    <dbReference type="PhylomeDB" id="Q97BI8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1290">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033573">
        <property type="entry name" value="transpos_IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360:SF2">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01321">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS200-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="121" mass="14319" checksum="D61C943AC81EF21C" modified="2001-10-01" version="1">MELKSTRHVKYLCNYHYLWIPKYRRAILTNEVAEYTKEVLKSIAKELGCEIIALEVMPDHIHLFVNCPPRYSPSYLTNYFKGKSARLILKKSPELRRYKKLWSRSYFVSTAGNVSSETIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BK0</accession>
    <name>Q97BK0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0443276 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0443276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BK0"/>
    <dbReference type="PaxDb" id="273116-14324670"/>
    <dbReference type="KEGG" id="tvo:TVG0443276"/>
    <dbReference type="HOGENOM" id="CLU_1954783_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="128" mass="14174" checksum="49E4770AA4D495C7" modified="2001-10-01" version="1">MPAIMPGLFALSGMISITPSPIFTLAFQRFATGSSWEKNGYNLSSFAMRLITLMTDLLSVSAAFAKVFGLISISPHINTGSLSKLFVTLIPVEIIFFTIFSCEGLLENLSYIYDHIHIKISLIYLNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BL1</accession>
    <name>Q97BL1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0433464</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BL1"/>
    <dbReference type="STRING" id="273116.gene:9381224"/>
    <dbReference type="PaxDb" id="273116-14324659"/>
    <dbReference type="DNASU" id="1440961"/>
    <dbReference type="KEGG" id="tvo:TVG0433464"/>
    <dbReference type="eggNOG" id="arCOG00042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_1_2"/>
    <dbReference type="PhylomeDB" id="Q97BL1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054306">
        <property type="entry name" value="TtuA-like_LIM_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22082">
        <property type="entry name" value="TtuA_LIM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="2-thiouridine synthetase TtuA-like N-terminal LIM" evidence="5">
        <location>
            <begin position="5"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="4">
        <location>
            <begin position="54"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="312" mass="35460" checksum="07F2A313C6F28EB4" modified="2001-10-01" version="1">MLVMKCSKCSAEAIYYAKYNGLYLCRDHFNEMIEGRVKKEIRKQVEFHGDDVTISVALSGGKDSSVTLFLLHKILGNRKNMKIKAFTVDEGIDGYRPSGLESAKRLCTSLGIDHETISFYDSYGLTLDSIVKTNPVKIPCAYCGPLRRKLINEMADKQDADYVALGLNLDDYSQSILMNVAKGDFDRFIRMSPQTDRKVGLVRRIAPLRTILEKEVVLYAVTNDIPFDSSWCPYYSRAQRNVFREVVNKLSEENPSTKFAILKFFDRVRENITTGHEEVKLKRCKICGAPTERDVCTACSSLQEVNEMMAEH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BQ0</accession>
    <name>COPP_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Bifunctional phosphopantetheine adenylyltransferase/NTP phosphatase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName>Phosphopantetheine adenylyltransferase</fullName>
                <ecNumber>2.7.7.3</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>Dephospho-CoA pyrophosphorylase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>Pantetheine-phosphate adenylyltransferase</fullName>
                <shortName>PPAT</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="2">Probable inosine/xanthosine triphosphatase</fullName>
                <ecNumber evidence="2">3.6.1.73</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2">Non-canonical purine NTP phosphatase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="2">Nucleoside-triphosphate phosphatase</fullName>
                <shortName evidence="2">NTPase</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name type="primary">coaD</name>
        <name type="ordered locus">TV0405</name>
        <name type="ORF">TVG0394586</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA/RNA precursor pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>XTP + H2O = XDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28406"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59884"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ITP + H2O = IDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28330"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the eukaryotic CoaD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the YjjX NTPase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3"/>
    <dbReference type="EC" id="3.6.1.73" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916661.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BQ0"/>
    <dbReference type="SMR" id="Q97BQ0"/>
    <dbReference type="STRING" id="273116.gene:9381183"/>
    <dbReference type="PaxDb" id="273116-14324620"/>
    <dbReference type="DNASU" id="1440919"/>
    <dbReference type="GeneID" id="1440919"/>
    <dbReference type="KEGG" id="tvo:TVG0394586"/>
    <dbReference type="eggNOG" id="arCOG01221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_887399_0_0_2"/>
    <dbReference type="OrthoDB" id="53228at2157"/>
    <dbReference type="PhylomeDB" id="Q97BQ0"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103023">
        <property type="term" value="F:ITPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02164">
        <property type="entry name" value="PPAT_CoAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000030">
        <property type="entry name" value="Bifunctional phosphopantetheine adenylyltransferase/NTP phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000376">
        <property type="entry name" value="Phosphopantetheine adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00648">
        <property type="entry name" value="Non_canon_purine_NTPase_YjjX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00647">
        <property type="entry name" value="PPAT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002786">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026533">
        <property type="entry name" value="NTPase/PRRC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023540">
        <property type="entry name" value="PPAT_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050299">
        <property type="entry name" value="YjjX_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001985">
        <property type="entry name" value="PRK00777.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002250">
        <property type="entry name" value="PRK01170.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699:SF2">
        <property type="entry name" value="NON-CANONICAL PURINE NTP PHOSPHATASE_PRRC1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01931">
        <property type="entry name" value="NTPase_I-T"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156330" description="Bifunctional phosphopantetheine adenylyltransferase/NTP phosphatase">
        <location>
            <begin position="1"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantetheine adenylyltransferase">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Inosine/xanthosine triphosphatase">
        <location>
            <begin position="153"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P39411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="328" mass="37665" checksum="48B3048F1EAF22E6" modified="2001-10-01" version="1">MITVVGGTFSKLHKGHKALLEKAIETGNEIVIGLTSDEYVKRNKVYPAIPYKERYRNLYNYMVKKTNKFRIRPIDDRNGNAPYERDYEIIVVSPETYQRSLKINEIRIQNGLPPLKIIRVPYVLAEDLFPISSTRIINGEIDGNGRRLKPVKVAIATNNSAKLKATNDFFHKLMKNFDVIQNTDYKLETQQPFGEVTMNMATKRAMQSLGDNDYAIGIESGIVYERFSRKYFDFHYCVVIDRFGNVTRGSSSGFEVPDRIIDLIKRDMSFSQAYGKVIDTNGIDDSTGIVGKISNGRVRRYDLIMECIRNAFIPRFDPDFYDTTYTPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BQ9</accession>
    <name>Q97BQ9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Na+/H+ antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG0383193</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916651.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BQ9"/>
    <dbReference type="STRING" id="273116.gene:9381173"/>
    <dbReference type="PaxDb" id="273116-14324611"/>
    <dbReference type="GeneID" id="1440909"/>
    <dbReference type="KEGG" id="tvo:TVG0383193"/>
    <dbReference type="eggNOG" id="arCOG01953">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_701363_0_0_2"/>
    <dbReference type="OrthoDB" id="12029at2157"/>
    <dbReference type="PhylomeDB" id="Q97BQ9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="236"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="16"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB59538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="393" mass="42971" checksum="91FF095B1F208800" modified="2001-10-01" version="1">MDLGILSQLLILLFVSMLLGVLFSKFKIPDVVAYILTGVLVGNGFLKIVSPSVPISDIESVSLFFIILAIGVEANTEYIYGNVRRSIILTISSFIAPSMLGFLYLQAVAHLPIIPAFVLSMSVGVPSISIISVLLMRYDLLKLNAGRLILSSVVLTDTSAFIILSSTYESVTKVLISFGFVVLFLFAIWLFDILIRRRAGRIIEWISRRETDNNMEYILFSLVIIAGLLVSTLFEFIGITYVLGAFYAGILIHEASIGQKYHKIILNTIKRINDSFFIPLFFSIAGISMILPKISIFMIFLPALLILMGASGGLTYLVSHRLIRSLEPIKVVSILGGRGAVGVIIATIAFQSGIIDVAVYSLALIGTVIFSLVMTPLVSIGNAPSQNDDVRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BS5</accession>
    <name>Q97BS5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0368277 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0368277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916634.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BS5"/>
    <dbReference type="STRING" id="273116.gene:9381157"/>
    <dbReference type="PaxDb" id="273116-14324595"/>
    <dbReference type="GeneID" id="1440892"/>
    <dbReference type="KEGG" id="tvo:TVG0368277"/>
    <dbReference type="eggNOG" id="arCOG05385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2695908_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="74" mass="8273" checksum="39CBB31BF1FC3447" modified="2001-10-01" version="1">MDIRRLAKEKRRSFGKVVAGIVLLVIAIPVFLDYKVFPVINSEIGPHQIGSWLALLFSFIGFILIIVGMGEMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BS7</accession>
    <name>Q97BS7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0367712 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0367712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916632.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BS7"/>
    <dbReference type="STRING" id="273116.gene:9381155"/>
    <dbReference type="PaxDb" id="273116-14324593"/>
    <dbReference type="GeneID" id="1440890"/>
    <dbReference type="KEGG" id="tvo:TVG0367712"/>
    <dbReference type="eggNOG" id="arCOG07406">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2875290_0_0_2"/>
    <dbReference type="OrthoDB" id="56709at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="63" mass="7016" checksum="CC99EB12D1540C86" modified="2001-10-01" version="1">MNRSVLAVYSIAGIQFVIAIILWILAVTNPTGNQRIWSVVFAIDLILSGIIAFIIMRPEMEVN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-28" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BW9</accession>
    <name>SUI1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0336</name>
        <name type="ORF">TVG0336952</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916590.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BW9"/>
    <dbReference type="SMR" id="Q97BW9"/>
    <dbReference type="STRING" id="273116.gene:9381113"/>
    <dbReference type="PaxDb" id="273116-14324551"/>
    <dbReference type="GeneID" id="1440848"/>
    <dbReference type="KEGG" id="tvo:TVG0336952"/>
    <dbReference type="eggNOG" id="arCOG04223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082805_6_1_2"/>
    <dbReference type="OrthoDB" id="11182at2157"/>
    <dbReference type="PhylomeDB" id="Q97BW9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11567">
        <property type="entry name" value="YciH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00604">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022851">
        <property type="entry name" value="SUI1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005872">
        <property type="entry name" value="SUI1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002096">
        <property type="entry name" value="PRK00939.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <feature type="chain" id="PRO_0000130594" description="Protein translation factor SUI1 homolog">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00604"/>
        </source>
    </evidence>
    <sequence length="100" mass="11447" checksum="DDE8461A88181086" modified="2001-10-01" version="1">MKDKNFTGMPKELQPWEGFGREAQAVKIVVDKRRYGKFVTIIEGIDPKIEDIEKIAKELKKKVASGGTVKEGRIIELQGDHRQEVKKFLEDMGFKVSVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BY0</accession>
    <name>Q97BY0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0329450 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0329450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BY0"/>
    <dbReference type="STRING" id="273116.gene:9381101"/>
    <dbReference type="PaxDb" id="273116-14324540"/>
    <dbReference type="KEGG" id="tvo:TVG0329450"/>
    <dbReference type="HOGENOM" id="CLU_3003370_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="56" mass="6269" checksum="C814486072E28722" modified="2001-10-01" version="1">MILKKYDTPSCYVGAINKAVALVKAYRKKCSSLEIDRDVNAFANIKKCSCMFQTSC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C15</accession>
    <name>PURA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylosuccinate synthetase</fullName>
            <shortName evidence="1">AMPSase</shortName>
            <shortName evidence="1">AdSS</shortName>
            <ecNumber evidence="1">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purA</name>
        <name type="ordered locus">TV0290</name>
        <name type="ORF">TVG0301512</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916545.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C15"/>
    <dbReference type="SMR" id="Q97C15"/>
    <dbReference type="STRING" id="273116.gene:9381064"/>
    <dbReference type="PaxDb" id="273116-14324504"/>
    <dbReference type="GeneID" id="1440803"/>
    <dbReference type="KEGG" id="tvo:TVG0301512"/>
    <dbReference type="eggNOG" id="arCOG04387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_2"/>
    <dbReference type="OrthoDB" id="372247at2157"/>
    <dbReference type="PhylomeDB" id="Q97C15"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.300.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002223">
        <property type="entry name" value="PRK01117.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000095281" description="Adenylosuccinate synthetase">
        <location>
            <begin position="1"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="21"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="22"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="47"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="49"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
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        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
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            <position position="230"/>
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        <ligand>
            <name>IMP</name>
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        <location>
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        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
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    <feature type="binding site" evidence="1">
        <location>
            <begin position="305"/>
            <end position="311"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="337"/>
            <end position="339"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="423"/>
            <end position="425"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <sequence length="436" mass="49007" checksum="156B5130A9ACE394" modified="2001-10-01" version="1">MSVNYSEDNNRTAVVVGLQFGDEGKGKITDFLSESYDVVVRFNGGTNAGHTVVVGNETYKFHLLPSGSLRTKVVVLGSGMVIDPQSLISEIEIVKKNNSNLAVVVSSNAHVVTKIHKYLDVEEEKIRSSMVIGTTAQGIGPTYEDKYARSGLRMIDLLDYKTIIEKLDIIYRMHENLLSRSEFSDPSVRENLAKELYSYGQELKKYMYYTDILINKLYNEGRTILFEGAQGVLLDPDFGFYPYVTSSNTISASSYTGTGFSMRKIKKVIGVAKAYVSKVGAGPFPTELNDDLAKKIRDAGGEYGTTTGRPRRVGWLDVPLLKYAVQIDDVDEIALTKVDTLGILHTIKVCYSYELDGKQIDYIPKHISEVSRVKPLYEEFEGWGDLSSSLLHPKMRKWEIPQQLRRYIDFIEEQIGRPVTIISSGKDRSKTVRIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C16</accession>
    <name>Q97C16_THEVO</name>
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        <submittedName>
            <fullName evidence="5">Bacterioferritin comigratory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0300659</name>
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    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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            <taxon>Thermoplasmata</taxon>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C16"/>
    <dbReference type="STRING" id="273116.gene:9381063"/>
    <dbReference type="PaxDb" id="273116-14324503"/>
    <dbReference type="GeneID" id="1440802"/>
    <dbReference type="KEGG" id="tvo:TVG0300659"/>
    <dbReference type="eggNOG" id="arCOG00310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_2_2"/>
    <dbReference type="OrthoDB" id="6924at2157"/>
    <dbReference type="PhylomeDB" id="Q97C16"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="PRX_AhpE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
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        <property type="entry name" value="Tpx_Peroxidase_subfamily"/>
    </dbReference>
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        <property type="entry name" value="THIOL PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43110:SF1">
        <property type="entry name" value="THIOL PEROXIDASE"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="3"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="3">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="157" mass="17564" checksum="F337B9A7DA43A41D" modified="2001-10-01" version="1">MSPKVNEKAPDFEAPDTALKMRKLSEFRGQNVVLAFFPGAFTSVCTKEMCTFRDSMANFNKFKAKVIGISVDSPFSLAEFAKKNNLTFDLLSDSNREISKKYDVLHQNFAGVPGLTASKRSVFIIDGDGIVRYAWVSDDPGKEPDYKAIQEFLSKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C24</accession>
    <name>Q97C24_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0293838 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0293838</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916536.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C24"/>
    <dbReference type="PaxDb" id="273116-14324495"/>
    <dbReference type="GeneID" id="1440794"/>
    <dbReference type="KEGG" id="tvo:TVG0293838"/>
    <dbReference type="eggNOG" id="arCOG05238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629483_0_0_2"/>
    <dbReference type="OrthoDB" id="129677at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="75" mass="8435" checksum="F66196E7ED5D574B" modified="2001-10-01" version="1">MKTYIKVSFSSEGARPSEVINRLRSLGFKPVIGEHDMVYEWGNGATPDDAIWFADKIHATLAGYNVRFEIETVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C28</accession>
    <name>Q97C28_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0290017 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0290017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59419.1"/>
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    <dbReference type="AlphaFoldDB" id="Q97C28"/>
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        <source>
            <dbReference type="EMBL" id="BAB59419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
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    </evidence>
    <sequence length="123" mass="13455" checksum="A3D09BA955CCBEC9" modified="2001-10-01" version="1">MSNLTTPGGICSPGYIKVDFPVPPYYIYVPTTSPTVARSPTAAVTSPLTDNCYTTYAPVLPVHNTQIPAIYPTLAFTFDIYKTPVSVPKFVVKHRTRKKAPERLEIPSSTILTVLGIFHVDTA</sequence>
</entry>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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            <taxon>Thermoplasmata</taxon>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
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    <dbReference type="PaxDb" id="273116-14324469"/>
    <dbReference type="GeneID" id="1440770"/>
    <dbReference type="KEGG" id="tvo:TVG0266641"/>
    <dbReference type="eggNOG" id="arCOG00609">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028221_0_1_2"/>
    <dbReference type="OrthoDB" id="19110at2157"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:peptidase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="359" mass="40291" checksum="7DEA616FB7BE59DF" modified="2001-10-01" version="1">MEPNIKVQSEDLRYIVQVVTSKIKVYDIIVDPVSVKFYFFDSDNEGIDASFDYIRRELVDKGFIPFLSRDGENFIQVTRRPKQRYFGNIVNVVLLVATILSTMYVGSQYSINFVPRGSFYELRLWAYGFLFFSAPLLLILGIHESAHFLVAKRHHVKASLPFFIPFPVGIGTFGAFISLRDPLPNRKAMTEIGAAGPIAGFLTALPLLFVADYFQGVIKPIPPYYIPFKVTFPLIYQLLGLHTNFTGPIFPMVFAVWVGMFATAMNLIPAGQLDGSHILRGVLGNRANVLSYVFLAFLFGIGFFYTGWWIIAIFVVFTGLVHPPALNDFSKISKLDIVLGIVSLAMFILTFTIVPIKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C53</accession>
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        <submittedName>
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        <name evidence="3" type="ORF">TVG0265894</name>
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            <taxon>Archaea</taxon>
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        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="74" mass="8237" checksum="4BFDFBC9A1B2EB67" modified="2001-10-01" version="1">MKTIKMRIYGMTCNDCVATVEKGLKSVDGVLWVSVSLPDGSAVVKVDDSVDPEKLEDAEVFKKTRYRGEVRDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C85</accession>
    <name>Q97C85_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C85"/>
    <dbReference type="STRING" id="273116.gene:9380989"/>
    <dbReference type="PaxDb" id="273116-14324432"/>
    <dbReference type="KEGG" id="tvo:TVG0226399"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_16_0_2"/>
    <dbReference type="PhylomeDB" id="Q97C85"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="375" mass="40826" checksum="17639BE29136FF82" modified="2001-10-01" version="1">MGLFVLVNYFGVVIFSKIINAITALKLVVPTITMVTLFLVSFKLRNFSAYGVAPYGPFSVVSAIVTSGIVYSYLGFQAVINLAGESRNPKRDGPLSLILVIIFSVVFYVVLQIAFIGSVPASDISKGWAFLSLPSPFADIAVSFNMFWLYTLIIADSIYSPSGSSMAAVASNSRNLYALSKNGTLPSFFLRVDNNTGIPRRALLFNFVISLIVLVTLKSWHQIIETLGILLLISFIGSAVSSGVIGSIKRYSSIKMPFIVIVSAIVFVFSGLIISISSFSKVLVVSLLLVPSLLLFAVSSGRGNRVIADVKAGLWFIIYVMEIAILSFIYNEHYVSGYIFLGAYLLSSTITFILARRSGVKFMHQAGEYDIAEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C90</accession>
    <name>Q97C90_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0220714</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulator TrmB family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916469.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C90"/>
    <dbReference type="STRING" id="273116.gene:9380983"/>
    <dbReference type="PaxDb" id="273116-14324427"/>
    <dbReference type="GeneID" id="1440729"/>
    <dbReference type="KEGG" id="tvo:TVG0220714"/>
    <dbReference type="eggNOG" id="arCOG02038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062979_2_0_2"/>
    <dbReference type="OrthoDB" id="30795at2157"/>
    <dbReference type="PhylomeDB" id="Q97C90"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09124">
        <property type="entry name" value="PLDc_like_TrmB_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021586">
        <property type="entry name" value="Tscrpt_reg_TrmB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11495">
        <property type="entry name" value="Regulator_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159071">
        <property type="entry name" value="TrmB C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="3">
        <location>
            <begin position="12"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription regulator TrmB C-terminal" evidence="4">
        <location>
            <begin position="112"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="40428" checksum="FC93FFCF01CF6C26" modified="2001-10-01" version="1">MSDEEEDAFSYLSKLGLTPYEIKIYKTLLIYGPKSATETAKLSRVPQPRVYDIFESLESKGAVEVSPGKKKIYRAVPVDNFIDRKLIEIKDYKQRIQSYIEKQKKFKETAPYLWMIKNNFQIREKMKDVITNSKYEIITSLNYENLRYLKRYFIDAVKKGLTVVLVVFPDAESDFLKTLPDDIVIKKRPGSASQVIISDRNDGIINVESKEDSEGYALYFNESELIHILNYYFYHTIWGPSEIYHDFPEKPDLKFTTAWLSCEAINSFLKRSYSITGSLEGIGFNGPVKINGTITGTDLVPGFKQTFFIKDGDRIYSVGGKTARMEDIKLTNLVINIWTPGERGKLGQNVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CA4</accession>
    <name>Q97CA4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enoyl-CoA hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0203661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2013-03" db="PDB data bank">
            <title>Crystal structure of enoyl-CoA hydratase from Thermoplasma volcanium GSS1.</title>
            <authorList>
                <consortium name="New York Structural Genomics Research Consortium (NYSGRC)"/>
                <person name="Shabalin I.G."/>
                <person name="Cooper D.R."/>
                <person name="Majorek K.A."/>
                <person name="Mikolajczak K."/>
                <person name="Porebski P.J."/>
                <person name="Stead M."/>
                <person name="Hillerich B.S."/>
                <person name="Ahmed M."/>
                <person name="Bonanno J."/>
                <person name="Seidel R."/>
                <person name="Almo S.C."/>
                <person name="Minor W."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.37 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916454.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="PDB" id="4JYL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.37 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-243"/>
    </dbReference>
    <dbReference type="PDBsum" id="4JYL"/>
    <dbReference type="AlphaFoldDB" id="Q97CA4"/>
    <dbReference type="SMR" id="Q97CA4"/>
    <dbReference type="STRING" id="273116.gene:9380968"/>
    <dbReference type="PaxDb" id="273116-14324412"/>
    <dbReference type="GeneID" id="1441684"/>
    <dbReference type="KEGG" id="tvo:TVG0203661"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_2"/>
    <dbReference type="OrthoDB" id="56751at2157"/>
    <dbReference type="PhylomeDB" id="Q97CA4"/>
    <dbReference type="EvolutionaryTrace" id="Q97CA4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="4JYL"/>
        </source>
    </evidence>
    <sequence length="243" mass="26367" checksum="3DFB7E94D74D6F1E" modified="2001-10-01" version="1">MLVEIERRGDASLIVLSRPEKLNAINLEMLADLADQFSKAEKEDTRVIVITGYGKNFSAGADINMLASFDPASAYSFRLKMNSIAQRIRKSDKPVIALLKGYSMGGGLELAESADIRIAMSDAVIGQPESSIGINAGAGGNVILPKLVGRGSAAYLAMSGKKLNAQEAMALGLVDEVVDDEAKAWKIIDDICKKPKKTLQFIKRAINSSYDMGLESAMDQEALYFSLLFTDPEVLDALSKWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CB9</accession>
    <name>Q97CB9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0189568</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CB9"/>
    <dbReference type="STRING" id="273116.gene:9380953"/>
    <dbReference type="PaxDb" id="273116-14324397"/>
    <dbReference type="KEGG" id="tvo:TVG0189568"/>
    <dbReference type="eggNOG" id="arCOG00511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056519_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CB9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="110"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="328" mass="37092" checksum="95994781937E0F03" modified="2001-10-01" version="1">MLRFMVRSFASLAISSAYSIPIGLSSVTSILDEDTIIERVCYGYLKCNATCSTEIVIVNYSITLNMSDDVVVKLLNDGYFSLDAGAYFGVVPKAIWSRKFQEIDNTVRLATNVLYITESDGTSILVDSGIGNKFDDKFRKIYRVEKQSDIYEYIRLHGDPNSVRMIINSHLHFDHVGHNADFKNAYAYAQADEFKAARYRNYITKANYRLSASQIKNKVEIGGSKRINGFIRVIKTDGHTPGHQVILINAGGRKIMYFGDIVPSTFHLKLPYRTAIDLDPLKTIEFKKNLVKMAIRENYICIFNHDVETPAAILSGDYSDPKYVKVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CC1</accession>
    <name>Q97CC1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Isopentenyl phosphate kinase</fullName>
            <shortName evidence="10">IPK</shortName>
            <ecNumber evidence="2 10">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">TVG0187430</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the formation of isopentenyl diphosphate (IPP), the building block of all isoprenoids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>isopentenyl phosphate + ATP = isopentenyl diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="2 10"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916436.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CC1"/>
    <dbReference type="STRING" id="273116.gene:9380951"/>
    <dbReference type="PaxDb" id="273116-14324395"/>
    <dbReference type="GeneID" id="1441666"/>
    <dbReference type="KEGG" id="tvo:TVG0187430"/>
    <dbReference type="eggNOG" id="arCOG00860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070213_0_0_2"/>
    <dbReference type="OrthoDB" id="15328at2157"/>
    <dbReference type="PhylomeDB" id="Q97CC1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04241">
        <property type="entry name" value="AAK_FomA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040647">
        <property type="entry name" value="IPPK_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="ISOPENTENYL PHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016496">
        <property type="entry name" value="Kin_FomA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="13">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="5"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR016496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAB59323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="244" mass="27611" checksum="018D2EA4C9C6343D" modified="2001-10-01" version="1">MIIIKLGGSLITEKNSYRVFRESETKKAIREIANDFEDFVIIHGGGSFGHIKAKEYGLPGEINKLSSAGFSVVHNDMSYLNHLVVSIMISEGLKPMTVPISSMIFNGNFDYTMLKKYHDLGFVPVSYGDVYVKNNNFYGIYSGDNIVLDLSKIFNPDFVAFLSDVDGIFDKDPKKYRDAKLLRTINTKVEFSKPENDVTGGIENKLNTMFLIRKYTKSVYLINGYYPERLKMIGNDNFTGTVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CD2</accession>
    <name>Q97CD2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KaiC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0180022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916425.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CD2"/>
    <dbReference type="SMR" id="Q97CD2"/>
    <dbReference type="STRING" id="273116.gene:9380940"/>
    <dbReference type="PaxDb" id="273116-14324384"/>
    <dbReference type="GeneID" id="1441655"/>
    <dbReference type="KEGG" id="tvo:TVG0180022"/>
    <dbReference type="eggNOG" id="arCOG01693">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163848_0_0_2"/>
    <dbReference type="OrthoDB" id="27015at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC-like" evidence="1">
        <location>
            <begin position="14"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="238" mass="27430" checksum="2717E9495D763693" modified="2001-10-01" version="1">MQAEGEKSDKISKISTGIPRVDELLYGGFDGRDNIVVSGNNFSQKRSFIYNFIKSSANSGMKVIIINVDGSSESLLADLARRGTDLENIILLDGFSKNFQEKVPPNAEVIDDPSNVSSVLKQYMLRAQKEKDFIFVFLSATPYLMDFNDQNRKFFVQLVHWNRKNSLLSTYVLDDFVGQTTAEWFAYMMDFSLYFKNDGDYDYMRVLSLKPVRTKDWIRIYKDSDVFTLGSFNVERIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CH3</accession>
    <name>Q97CH3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0135483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CH3"/>
    <dbReference type="STRING" id="273116.gene:9380897"/>
    <dbReference type="PaxDb" id="273116-14324342"/>
    <dbReference type="DNASU" id="1441612"/>
    <dbReference type="KEGG" id="tvo:TVG0135483"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_538227_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CH3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="210"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="502" mass="56317" checksum="1060B25249CA6EC6" modified="2001-10-01" version="1">MGMGEAEVKQENTLYRYLYQIAEKNSSQVAIYYLGKPITYSALISMVDSFAFNLKSLDVDGDVGIMLRNSPQILISILALNRLGLPAFILNQYENPSKYGIKTIIGNASSLCRLDPFPDRAIVVREEDLGPYGSSIDPYFKNDRACFKKFLAKRGILKFSDMIFDKVNREELPENDGVLKRHTFRGDSVYTYPSSFIYEAANSLQQFLEGYRKDVAVYSSLNHISSLIFSLIPLFSGRTLTLMPEFYEVEKTIKAIEKAESSMLVGNTYLYEEMLLKNVEMPKTIRYLFSIGEYIRPEFLKDFFVKYGKDIKIGYDISAATGLVCMQELMKNDFDSVGVALPDCTVSISDRSGKPLPVGENGLVKIKSNRIVDKTDEGGSLSTGDLGHIDESGHLYLAKASETVFGSVVSPEIIEGIIGKVWNKTDLAVGLYKTRSSYRLEAFIASSDRSDAVKFARTCKEILPLEIRPFRYVMVKKIPRSQSGKIIRELLGKESIGSINPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CH7</accession>
    <name>Q97CH7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0132220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CH7"/>
    <dbReference type="STRING" id="273116.gene:9380892"/>
    <dbReference type="PaxDb" id="273116-14324338"/>
    <dbReference type="KEGG" id="tvo:TVG0132220"/>
    <dbReference type="eggNOG" id="arCOG02682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_14_2"/>
    <dbReference type="PhylomeDB" id="Q97CH7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="475"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="100"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="546" mass="60275" checksum="AD519403ED29F6EF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CN6</accession>
    <name>Q97CN6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="2">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="2">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0066474</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916322.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CN6"/>
    <dbReference type="STRING" id="273116.gene:9380831"/>
    <dbReference type="PaxDb" id="273116-14324279"/>
    <dbReference type="GeneID" id="1441552"/>
    <dbReference type="KEGG" id="tvo:TVG0066474"/>
    <dbReference type="eggNOG" id="arCOG01736">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081251_3_2_2"/>
    <dbReference type="OrthoDB" id="44091at2157"/>
    <dbReference type="PhylomeDB" id="Q97CN6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:RNA 2',3'-cyclic 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014051">
        <property type="entry name" value="Phosphoesterase_HXTX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561:SF1">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02834">
        <property type="entry name" value="LigT_PEase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoesterase HXTX" evidence="3">
        <location>
            <begin position="16"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoesterase HXTX" evidence="3">
        <location>
            <begin position="108"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 1" evidence="2">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="2">
        <location>
            <begin position="114"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="164" mass="18562" checksum="36085B60FE06D964" modified="2001-10-01" version="1">MRAFIAIPIDPSSYMKNILSTFRQSRNIKVVEDTNVHITLKFLGDIDDDFAKKICSGLSGLKVHKFTIKTLGIGAFPNMRSPRVIFLDLQSNDIEKLYIEIGRIVGIDKDFVPHITIGRIKAKVRIPPLEFTQEEYEAKEVCLFSSRLTPSGPIYSRVCCKALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CQ9</accession>
    <name>Q97CQ9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG0045165 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0045165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CQ9"/>
    <dbReference type="SMR" id="Q97CQ9"/>
    <dbReference type="STRING" id="273116.gene:9380807"/>
    <dbReference type="PaxDb" id="273116-14324256"/>
    <dbReference type="DNASU" id="1441529"/>
    <dbReference type="KEGG" id="tvo:TVG0045165"/>
    <dbReference type="eggNOG" id="arCOG00683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127458_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CQ9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="35"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="169" mass="18724" checksum="926AE2EA2F397BB3" modified="2001-10-01" version="1">MEEFKNKGVGEIAIGYPKEISKDHGNKLTVNFWNYGYIIRRFEGVGEELGVKVVKVDEAWTSKTCSLCGEAHDDGRIKRGLYRCLRIGKVINADLNGAINILHIPESLGAGSRGQLTVRDRGNGLKTQPAVYRWTNGAGWVSSPTSYEVMKMKAVNCKPMNRHKGTLAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-15" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CU1</accession>
    <name>RL31_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L31e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl31e</name>
        <name type="ordered locus">TV0010</name>
        <name type="ORF">TVG0008932</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916267.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CU1"/>
    <dbReference type="SMR" id="Q97CU1"/>
    <dbReference type="STRING" id="273116.gene:9380775"/>
    <dbReference type="PaxDb" id="273116-14324224"/>
    <dbReference type="GeneID" id="1441495"/>
    <dbReference type="KEGG" id="tvo:TVG0008932"/>
    <dbReference type="eggNOG" id="arCOG04473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112570_3_2_2"/>
    <dbReference type="OrthoDB" id="10127at2157"/>
    <dbReference type="PhylomeDB" id="Q97CU1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00410">
        <property type="entry name" value="Ribosomal_eL31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_eL31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002258">
        <property type="entry name" value="PRK01192.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000153809" description="Large ribosomal subunit protein eL31">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="89" mass="10259" checksum="3FD6B3C5001D432A" modified="2001-10-01" version="1">MPDDEVSKEIIMNIPLREAKLSSRKRRADTSISILRNYVSKKLKVDTSKIWIDPKVSETIWKKGREKPPSKLSLKIIKLEEGTTEIILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q977Y2</accession>
    <name>Q977Y2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thermopsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0251055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
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                <person name="Suzuki M."/>
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            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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        <property type="protein sequence ID" value="BAB60639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_021792163.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978C0"/>
    <dbReference type="STRING" id="273116.gene:9382311"/>
    <dbReference type="PaxDb" id="273116-14325736"/>
    <dbReference type="GeneID" id="30928350"/>
    <dbReference type="KEGG" id="tvo:TVG1548715"/>
    <dbReference type="eggNOG" id="arCOG06951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158388_0_0_2"/>
    <dbReference type="OrthoDB" id="57340at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="131" mass="14582" checksum="0E8441D894376DED" modified="2001-10-01" version="1">MRYENIYKSLLFYIVGLALLYVSIFLSNNLKFNGNFISALPIVLPLVFSIASIGVAVIFIMEKDSPWLFRTGMMSLVSGITLFSFGVLAFYLGVKSLVWAGSFVIGIMLIFAAMVRLFIQGGLSAYRKSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q978D8</accession>
    <name>Q978D8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1530142</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978D8"/>
    <dbReference type="STRING" id="273116.gene:9382293"/>
    <dbReference type="PaxDb" id="273116-14325718"/>
    <dbReference type="GeneID" id="1442170"/>
    <dbReference type="KEGG" id="tvo:TVG1530142"/>
    <dbReference type="eggNOG" id="arCOG03626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088957_3_0_2"/>
    <dbReference type="OrthoDB" id="57556at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038730">
        <property type="entry name" value="HyfE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38601">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38601:SF1">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24563" checksum="55C304521A033A2A" modified="2001-10-01" version="1">MNNIDTVVYLISSLIAISAFYIQGQEFIASMIRGQSVQSVLIAVVAFIIGWQERSYDFFILGILIIILRAFLVNYFLQKRIPKQKVYLYERKVSLSYLFLLDLIFIVISVFIIYSIAFSGIVLHSFLGNGGNSSVLVFPLTLFFQGLFLIASRRTTFTHVIGYVEEENSLVLFAMFLLPIPIIIEASVFLDVLALVVISSIVVLEKFTHEPMEELIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q978H5</accession>
    <name>Q978H5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1488917 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1488917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978H5"/>
    <dbReference type="STRING" id="273116.gene:9382251"/>
    <dbReference type="PaxDb" id="273116-14325679"/>
    <dbReference type="KEGG" id="tvo:TVG1488917"/>
    <dbReference type="HOGENOM" id="CLU_2968650_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="58" mass="6896" checksum="8ADF77E80ABFCF83" modified="2001-10-01" version="1">MMQEQGIIMVRNLGSERIKYQFYMKKYQCSNLVGIVNGLFYYFTIFSCTIYSELMIMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q978J3</accession>
    <name>Q978J3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1469397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917654.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978J3"/>
    <dbReference type="STRING" id="273116.gene:9382231"/>
    <dbReference type="PaxDb" id="273116-14325661"/>
    <dbReference type="GeneID" id="1442112"/>
    <dbReference type="KEGG" id="tvo:TVG1469397"/>
    <dbReference type="eggNOG" id="arCOG00147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_745192_0_0_2"/>
    <dbReference type="OrthoDB" id="359492at2157"/>
    <dbReference type="PhylomeDB" id="Q978J3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17353">
        <property type="entry name" value="MFS_OFA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF304">
        <property type="entry name" value="MFS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="375" mass="40942" checksum="ACD3F4C2582D80BD" modified="2001-10-01" version="1">MKRCYLIIALLVMCFNSLYQYSWNVFEPLLAKGFGVEIVSVQIAFTLFAVFSTTFQGIGGYFADRDGPRNVSIAAALMSAFGFVASSLVHSIYLFYVIWSIGSIGEGILYGIATNLAVKWYSGRRGFAVGFVSLGFGLGSSIANIFLEKASEFRFPMMFIGLAELIVLPLLLSFVKYPSSGLTGERPSRNIKNPRFWILYLSFVFGSIPLIVISSSFGFIGSKLPELEFAVLVSIFPLLSGISRPIIGAISDAIGRPASVLLIDVAIFIGALFLVLRNFIFAVVLIGFFGGSMISLYFSLIGDVFGTRFSTANNGIFYTGKAVSGFMGSTLFAIIFTLGVEYSFYFVFISAIFGIAFLIISMAIVHAHFYPSAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q978L1</accession>
    <name>Q978L1_THEVO</name>
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        <submittedName>
            <fullName evidence="9">TVG1451111 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1451111</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978L1"/>
    <dbReference type="STRING" id="273116.gene:9382213"/>
    <dbReference type="PaxDb" id="273116-14325643"/>
    <dbReference type="KEGG" id="tvo:TVG1451111"/>
    <dbReference type="eggNOG" id="arCOG01364">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_478700_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Cobalamin-binding domain"/>
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        <property type="entry name" value="BchE-like"/>
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        <property type="entry name" value="Cobalamin-bd"/>
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        <property type="entry name" value="Methyltransferase_Class_B"/>
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        <property type="entry name" value="rSAM"/>
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        <property type="entry name" value="BLL1977 PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF04055">
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    </dbReference>
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        <property type="entry name" value="B12-binding_domain_containing"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
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    <feature type="domain" description="B12-binding" evidence="8">
        <location>
            <begin position="29"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="563" mass="65650" checksum="110D071BF9EAF2B0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q978N9</accession>
    <name>Q978N9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pyruvate formate-lyase activating enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">TVG1422495</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
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    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044176_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q978N9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:catalytic activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
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    <dbReference type="InterPro" id="IPR027596">
        <property type="entry name" value="AmmeMemoSam_rS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04337">
        <property type="entry name" value="AmmeMemoSam_rS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352:SF5">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE 1-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01101">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="90"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="367" mass="41308" checksum="C36468F27764154F" modified="2001-10-01" version="1">MIQDAKGINARSGSLCIMFVSVRKPSKAVLFKKEGETVVCTACRRYCRLKNNQIGFCGVRKNINGNLYLLVYGMPAAINIDPIEKKPILHMYPNSLIYSISTVGCSFACQYCQNYDISQRRNIEGFDYSPEEIVNSAIEDGCRGIAYTYNEPSIFMEFAHDVGILARKRGLINIFVTNGYETPEAVEYSKDFLDAATVDFKGNASNDFYRKYISVPSADPIFDTIKLMKESGIHVEITDLVVPRVGDKLSDARSMLERLISILGYEFPISFLRFHPDYKMMDLPPTPLETLKAHYNLAVSMGLKYVYIGNVPGYYEDTYCPNCHSLLIKRHGFNSSVVGLNDDGACKKCGYRTGILIGNNNRIDYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Q1</accession>
    <name>Q978Q1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prepilin type IV endopeptidase peptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1412448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917598.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Q1"/>
    <dbReference type="STRING" id="273116.gene:9382172"/>
    <dbReference type="TCDB" id="1.A.54.6.1">
        <property type="family name" value="the presenilin er ca(2+) leak channel (presenilin) family"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325603"/>
    <dbReference type="DNASU" id="1441480"/>
    <dbReference type="GeneID" id="1441480"/>
    <dbReference type="KEGG" id="tvo:TVG1412448"/>
    <dbReference type="eggNOG" id="arCOG07367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207675_0_0_2"/>
    <dbReference type="OrthoDB" id="57252at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25465" checksum="807D9696125B2AC2" modified="2001-10-01" version="1">MNGFIPIIASIAISLPLLIFGSHSDVKKRSINSFTFLPIFILALAFYILDKDVVMSIVTILSTVAVFIKPNIYVYIVLPLIIIGIGFFDTINLLTILIVGMFLLTGFGELLFGIGDIKGIVSVVLLFSSIPRFNNYIPFSIVFVFFIAVASGGALLYFVVYARLNGLKLRGLNVLYDEHEYLRNTIKYQLKDTNSGKVMIYRVPFLVPILVSTVLSLIAQLIIYASIHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q978T5</accession>
    <name>Q978T5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TVG1372758 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1372758</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917565.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978T5"/>
    <dbReference type="STRING" id="273116.gene:9382138"/>
    <dbReference type="PaxDb" id="273116-14325569"/>
    <dbReference type="GeneID" id="1441447"/>
    <dbReference type="KEGG" id="tvo:TVG1372758"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1575040_0_0_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="PhylomeDB" id="Q978T5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="2">
        <location>
            <begin position="38"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="169" mass="19098" checksum="85029DDD523A8B06" modified="2001-10-01" version="1">MGIRGLDLSDLFVSGASYKRLGSEEMMRSTFQKLDREIAAIFLGLSDGTISKAIEYSKVRTAFDHPLKDYSPIFDDLVSLKMEIDVLRFYLYGSGDEYSRALIKIKAKDLSLRATRVSLQTHGGYGYIHDFGIEKYYRDAMALSVYFGQDKYENMVLSDHIFGEAGGII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-31" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q978U3</accession>
    <name>TRF2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Tricorn protease-interacting factor F2</fullName>
            <ecNumber>3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trf2</name>
        <name type="ordered locus">TV1322</name>
        <name type="ORF">TVG1363786</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Proteases F1, F2 and F3 degrade oligopeptides produced by Tricorn (themselves probably produced by the proteasome), yielding free amino acids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Part of the Tricorn proteolytic complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917557.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978U3"/>
    <dbReference type="SMR" id="Q978U3"/>
    <dbReference type="STRING" id="273116.gene:9382130"/>
    <dbReference type="MEROPS" id="M01.020"/>
    <dbReference type="PaxDb" id="273116-14325561"/>
    <dbReference type="GeneID" id="1441439"/>
    <dbReference type="KEGG" id="tvo:TVG1363786"/>
    <dbReference type="eggNOG" id="arCOG02969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003705_0_1_2"/>
    <dbReference type="OrthoDB" id="139771at2157"/>
    <dbReference type="PhylomeDB" id="Q978U3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00142">
        <property type="entry name" value="ZINC_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000095111" description="Tricorn protease-interacting factor F2">
        <location>
            <begin position="1"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="236"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="783" mass="88980" checksum="57CDF0F111C31054" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-16" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q978U9</accession>
    <name>HEMH_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ferrochelatase</fullName>
            <ecNumber evidence="1">4.98.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heme synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Protoheme ferro-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemH</name>
        <name type="ordered locus">TV1316</name>
        <name type="ORF">TVG1357926</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>heme b + 2 H(+) = protoporphyrin IX + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.98.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="4.98.1.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917551.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978U9"/>
    <dbReference type="SMR" id="Q978U9"/>
    <dbReference type="STRING" id="273116.gene:9382124"/>
    <dbReference type="PaxDb" id="273116-14325555"/>
    <dbReference type="GeneID" id="1441433"/>
    <dbReference type="KEGG" id="tvo:TVG1357926"/>
    <dbReference type="eggNOG" id="arCOG05373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018884_2_1_2"/>
    <dbReference type="OrthoDB" id="56534at2157"/>
    <dbReference type="PhylomeDB" id="Q978U9"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF1">
        <property type="entry name" value="FERROCHELATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000175238" description="Ferrochelatase">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00323"/>
        </source>
    </evidence>
    <sequence length="304" mass="35231" checksum="68494CEA42576624" modified="2001-10-01" version="1">MKTAVLLLSYGSPEKMSDIDEYLSKIFGGKPVPKGVAEENYRKYEMFGGLSPSNRIIQSIRDRLQKRFDQSGDVDVFTAFKHWYPSIGEVVPDLKGYDNIVSIPLFSFFSENVKASYYKPLAEALEKNDIRTKMEFVNGISNYDLFIPMWIHLIEEKEKGDSFYLFDAHSLPHPENEEDYLFWLRYSTYKITQIMGLRSSDFGFQGGHEGWLGPSIYNVYRKAKEKKIIAVPISFLYDHLEILYDLDYEFRKKIEEDGYSYERVPMPNDSAIFITLLERIVSSSITHLSGELIGNGKEKSENFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-20" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q978V5</accession>
    <name>TPIS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Triosephosphate isomerase</fullName>
            <shortName evidence="1">TIM</shortName>
            <shortName evidence="1">TPI</shortName>
            <ecNumber evidence="1">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tpiA</name>
        <name type="ordered locus">TV1310</name>
        <name type="ORF">TVG1351870</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB60452" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60452.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917545.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978V5"/>
    <dbReference type="SMR" id="Q978V5"/>
    <dbReference type="STRING" id="273116.gene:9382118"/>
    <dbReference type="PaxDb" id="273116-14325549"/>
    <dbReference type="GeneID" id="1441427"/>
    <dbReference type="KEGG" id="tvo:TVG1351870"/>
    <dbReference type="eggNOG" id="arCOG01087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104921_0_0_2"/>
    <dbReference type="OrthoDB" id="9465at2157"/>
    <dbReference type="PhylomeDB" id="Q978V5"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_A">
        <property type="entry name" value="TIM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022891">
        <property type="entry name" value="Triosephosphate_isomerase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003302">
        <property type="entry name" value="PRK04302.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000090350" description="Triosephosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="1">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="213" mass="23290" checksum="3A58434E7C051FFD" modified="2002-06-20" version="2">MFTVIVNLKNYAEANGKNFVEFVSKLPKYDSVRLIIAPAILDLHNAHEFRNVEFFSQHVDDVGYGPYTGHIAIESLMNYGIIGSLLNHSERRLGEDKIISTVKKAQMLGFEIALCVESMEEAKRYSALKPSFIAYEPKELIGGNVSVSTAKPEIISEIVDICGTEGVPVLVGAGIKNRQDVRKSLDLGAQGILVSSGVVKSPDPVRSLNSLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q978V7</accession>
    <name>Q978V7_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1350001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917543.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978V7"/>
    <dbReference type="SMR" id="Q978V7"/>
    <dbReference type="STRING" id="273116.gene:9382115"/>
    <dbReference type="PaxDb" id="273116-14325547"/>
    <dbReference type="DNASU" id="1441425"/>
    <dbReference type="GeneID" id="1441425"/>
    <dbReference type="KEGG" id="tvo:TVG1350001"/>
    <dbReference type="eggNOG" id="arCOG00511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_3_2_2"/>
    <dbReference type="OrthoDB" id="7773at2157"/>
    <dbReference type="PhylomeDB" id="Q978V7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07729">
        <property type="entry name" value="AHL_lactonase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978:SF2">
        <property type="entry name" value="102 KBASES UNSTABLE REGION: FROM 1 TO 119443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="45"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="271" mass="30525" checksum="7E769DEDF8EF16E7" modified="2001-10-01" version="1">MPASKIYLLDYGWLGGDIGWFLPGQLGGAMTNSERNPRRDWVEIPITGALIDHPDARILFDTGIAPDAMKTHERGLMEAFPITKMDDQNHIENQLKLIGLKPEDIDFVVISHLHLDHIGQAAVFKDSHVPIIVQKKELESALSLIWQGKSGAYDYSDLLPLKGAAWMPLDDPVFEIVDGVYAEFTGGHTAGHQVLTVKTEKGSMYTLTGDFLHLPKEYDVEAKGWLLSNATEWNSYIKKMKIREKARKQKLIISHDPGIWDKYPHAPKFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q978Y5</accession>
    <name>Q978Y5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1320830 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1320830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978Y5"/>
    <dbReference type="STRING" id="273116.gene:9382086"/>
    <dbReference type="PaxDb" id="273116-14325518"/>
    <dbReference type="KEGG" id="tvo:TVG1320830"/>
    <dbReference type="eggNOG" id="arCOG07403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_856906_0_0_2"/>
    <dbReference type="OrthoDB" id="55773at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043958">
        <property type="entry name" value="Ta1207"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19020">
        <property type="entry name" value="Ta1207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="324" mass="37889" checksum="4E17E247EB0194EC" modified="2001-10-01" version="1">MINMVLQDIDDIFDSQAVLKGYLNSDLTVINEKYGLFYLMLPTIDESKQVSFYVFLNDDEIPEKHREEIEAVIGTYKPTFKNGVWKIYLNTEKFEFSEPFSKYFDVDSIVFDMASMKNGELILPIRFMSRDKEALVDSIVESAGYGNSIYLHYLGQNKGFEYSFTAIKLLDQIYRISIFVDNPSSLHGIYEETKRSIAWRRESKAPYKDNTEDYIYALDDSHSIPELLIDTAYTGEKGTKYIGKHSNYDIYRAFFGDALANHMSNIMMAENVYYLRRWSKYEDGKLYLYFYTTDDLLKIIPSVIKKTRESFTRVHINVEEITTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q979D7</accession>
    <name>Q979D7_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Penicillin G acylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG1261565</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S45 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917458.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979D7"/>
    <dbReference type="STRING" id="273116.gene:9382029"/>
    <dbReference type="MEROPS" id="S45.003"/>
    <dbReference type="PaxDb" id="273116-14325462"/>
    <dbReference type="GeneID" id="1441340"/>
    <dbReference type="KEGG" id="tvo:TVG1261565"/>
    <dbReference type="eggNOG" id="arCOG04082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011790_4_0_2"/>
    <dbReference type="OrthoDB" id="56056at2157"/>
    <dbReference type="PhylomeDB" id="Q979D7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017000">
        <property type="term" value="P:antibiotic biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.439.10">
        <property type="entry name" value="Penicillin Amidohydrolase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014395">
        <property type="entry name" value="Pen/GL7ACA/AHL_acylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043147">
        <property type="entry name" value="Penicillin_amidase_A-knob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023343">
        <property type="entry name" value="Penicillin_amidase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043146">
        <property type="entry name" value="Penicillin_amidase_N_B-knob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002692">
        <property type="entry name" value="S45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34218:SF4">
        <property type="entry name" value="ACYL-HOMOSERINE LACTONE ACYLASE QUIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34218">
        <property type="entry name" value="PEPTIDASE S45 PENICILLIN AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01804">
        <property type="entry name" value="Penicil_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001227">
        <property type="entry name" value="Pen_acylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB60366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="862" mass="97272" checksum="04CD6679728A2FA0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q979E2</accession>
    <name>Q979E2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1253183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917453.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979E2"/>
    <dbReference type="STRING" id="273116.gene:9382024"/>
    <dbReference type="PaxDb" id="273116-14325457"/>
    <dbReference type="GeneID" id="1441335"/>
    <dbReference type="KEGG" id="tvo:TVG1253183"/>
    <dbReference type="eggNOG" id="arCOG01143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074761_1_1_2"/>
    <dbReference type="OrthoDB" id="9937at2157"/>
    <dbReference type="PhylomeDB" id="Q979E2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050126">
        <property type="entry name" value="Ap4A_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011152">
        <property type="entry name" value="Pesterase_MJ0912"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850:SF2">
        <property type="entry name" value="BLL5683 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000883">
        <property type="entry name" value="Pesterase_MJ0912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="229" mass="26560" checksum="A0AFBF8FA8D2115D" modified="2001-10-01" version="1">MKVIIYSDIHANLEAMKEVIKREKFDIAIFLGDVVDYGPKPAETLDLVMENMDYGVMGNHDYAAATGEDCNCAPDMHDLSEFSRKEVTLPSLSSEDLKRLSSLKEFVDTEIDGRKILMTHASPNNHLFGYLFATEAEMVWKDKRYSSYDLIMVGHTHFQMLYRNKIINPGSAGQPRDGDWRPMYAIWDTDADEVIFKRFKYDNNTTWKQIKEIIPEGSPYLDQLRKFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q979G8</accession>
    <name>Q979G8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-hydroxy-3-methylglutaryl-coenzyme A reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1221182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917426.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979G8"/>
    <dbReference type="STRING" id="273116.gene:9381994"/>
    <dbReference type="PaxDb" id="273116-14325431"/>
    <dbReference type="GeneID" id="1441308"/>
    <dbReference type="KEGG" id="tvo:TVG1221182"/>
    <dbReference type="eggNOG" id="arCOG04260">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033422_0_0_2"/>
    <dbReference type="OrthoDB" id="10981at2157"/>
    <dbReference type="PhylomeDB" id="Q979G8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00644">
        <property type="entry name" value="HMG-CoA_reductase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004553">
        <property type="entry name" value="HMG_CoA_Rdtase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00532">
        <property type="entry name" value="HMG_CoA_R_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01192">
        <property type="entry name" value="HMG_COA_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="419" mass="45244" checksum="7356064AF07849F4" modified="2001-10-01" version="1">MVSSEVSGFYKMTVDERLNFVKEFSRLSDDDIRILRTNGSLAIDKADKIIENVITTIEMPLGIAVNFLINGKDYLIPMAIEEPSVVAAASNAAKVARKSGGFNAYATEPVMIGEIQVLGIDSLYLAKMNILRDKSRILELANTRSKTLSSLGAGAKDIEVNVYERPHRMLIVHLIVDVRDAMGANVVNSMCEYVAPEIEKITGGRVNLRILSNLTKYRVAYASAVFSKDIIGKEAVDNIVEAYRMAVVDIYRASTNNKGIMNGIDAVLVATMNDWRAAEANAHSYAALEGYGPLAKYEKNSNGDLVGTIEIPIAVGTVGGTTGSIDKARIARKILGVSNATEFAGVLAAVGLAQNFSAVRALATEGIQRGHMELHARNLALSAGASPEEVDSVVNEMISSKEITMSNAIKILEKMRKTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q979K2</accession>
    <name>RL13_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl13</name>
        <name type="ordered locus">TV1159</name>
        <name type="ORF">TVG1187479</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917393.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979K2"/>
    <dbReference type="SMR" id="Q979K2"/>
    <dbReference type="STRING" id="273116.gene:9381959"/>
    <dbReference type="PaxDb" id="273116-14325397"/>
    <dbReference type="GeneID" id="1441275"/>
    <dbReference type="KEGG" id="tvo:TVG1187479"/>
    <dbReference type="eggNOG" id="arCOG04242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076922_1_0_2"/>
    <dbReference type="OrthoDB" id="7668at2157"/>
    <dbReference type="PhylomeDB" id="Q979K2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
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    <feature type="chain" id="PRO_0000261853" description="Large ribosomal subunit protein uL13">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
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    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="136" mass="15242" checksum="F7AF29924DEA339B" modified="2001-10-01" version="1">MKIIDASNTVYGRLSAYVAKQLLNGEEVVIVNASKAVITGDRKFIIDKFKQRLDIGSVRKGPYYPKTPENILRRSIGDMLPKKITRGKEALSRCKVYRNLPKNVSSEKIEKVDDVMTDKVVGIITLEELSKELGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q979N1</accession>
    <name>Q979N1_THEVO</name>
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        <submittedName>
            <fullName evidence="8">NADH dehydrogenase I chain K</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="ORF">TVG1158465</name>
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    <organism evidence="8 9">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 4 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
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    <dbReference type="PaxDb" id="273116-14325367"/>
    <dbReference type="DNASU" id="1441245"/>
    <dbReference type="GeneID" id="1441245"/>
    <dbReference type="KEGG" id="tvo:TVG1158465"/>
    <dbReference type="eggNOG" id="arCOG01538">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_4_2_2"/>
    <dbReference type="OrthoDB" id="19089at2157"/>
    <dbReference type="PhylomeDB" id="Q979N1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:ATP synthesis coupled electron transport"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PRK05846.2-2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Proton_antipo_M"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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            <end position="45"/>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
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        <location>
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            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="288"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="121"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAB60271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="516" mass="56718" checksum="7986F3FAC93D180F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q979N5</accession>
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        <submittedName>
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            <taxon>Thermoplasmata</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="entry name" value="Thermoplasma acidophilum protein TA0956"/>
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    </dbReference>
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    <dbReference type="Pfam" id="PF11513">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="115" mass="13233" checksum="6CCC4938602AADD5" modified="2001-10-01" version="1">MYCGNMELCAMYNISIENLHPTTICVVMDKFLDSFAELLGVLEDQDQDELMDFISRYARTDEIRPEDKTVGFVVINSAKKMMSVSFSDIDENVKEKIREIIKPYRDSGYSVEADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Q4</accession>
    <name>Q979Q4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphoserine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.3</ecNumber>
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    <gene>
        <name evidence="9" type="ORF">TVG1139499</name>
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    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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            <taxon>Thermoplasmata</taxon>
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            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917340.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Q4"/>
    <dbReference type="SMR" id="Q979Q4"/>
    <dbReference type="STRING" id="273116.gene:9381904"/>
    <dbReference type="PaxDb" id="273116-14325344"/>
    <dbReference type="DNASU" id="1441222"/>
    <dbReference type="GeneID" id="1441222"/>
    <dbReference type="KEGG" id="tvo:TVG1139499"/>
    <dbReference type="eggNOG" id="arCOG01158">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036368_4_5_2"/>
    <dbReference type="OrthoDB" id="10041at2157"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAD-SF_hydro_IB_PSP-like_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0718">Serine biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="212" mass="24913" checksum="8EC3C1D1CEB785CA" modified="2001-10-01" version="1">MIIDEISLFILDMDGTLTLEPSSWDYVHRRLGINNHSVTRLYRNRYINYYCFFQSDIKAWLHKYPNLKKEKVVEFLREVPIRHGADELINVLRKNGIRTAVISGGISWLFDIISERSKIDYNFSNEIFTDEYGYIVPEGKVRVIPEEKDLVVRKIQEELGIGKDQTASVGDEMENDRIHRYSKYRFIISKKARDNLIPIPSGDLEDLLRILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Q9</accession>
    <name>Q979Q9_THEVO</name>
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        <submittedName>
            <fullName evidence="2">TVG1133289 protein</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="ORF">TVG1133289</name>
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        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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    <dbReference type="OrthoDB" id="56815at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="53" mass="6406" checksum="C0BE30E487B4FD4E" modified="2001-10-01" version="1">MKYNNEEKIKEVEALNREVKSMRKELDDVKEVLRGLIQVIMSRDDNFDDDEYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q979R2</accession>
    <name>Q979R2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArsR family transcriptional regulator</fullName>
        </recommendedName>
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        <name evidence="1" type="ORF">TVG1130521</name>
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    <organism evidence="1 2">
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979R2"/>
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    <dbReference type="PaxDb" id="273116-14325336"/>
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    <dbReference type="HOGENOM" id="CLU_129660_0_0_2"/>
    <dbReference type="OrthoDB" id="49861at2157"/>
    <dbReference type="PhylomeDB" id="Q979R2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014517">
        <property type="entry name" value="ArsR_tscrpt_regulator"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09824">
        <property type="entry name" value="ArsR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022057">
        <property type="entry name" value="UCP022057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="18771" checksum="5B2FEBC026B975CB" modified="2001-10-01" version="1">MVSRIKVVNDPGELVSIFHAADSDIKRKLLLDLSTGWITLPTIEQKYGKEGRRALLYLDKIKMIETQWITGSNGPEKAYHTYYTNVQINLMGTLSELADIIYAATLSDDQLQEYEDKIKALMKDDGSVFLGDVSDALNISQTLIRGIVKRSSILEIKGYKIELASGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q979S4</accession>
    <name>Q979S4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-acyl carrier protein reductase</fullName>
        </submittedName>
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        <name evidence="3" type="ORF">TVG1116741</name>
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    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979S4"/>
    <dbReference type="SMR" id="Q979S4"/>
    <dbReference type="STRING" id="273116.gene:9381882"/>
    <dbReference type="PaxDb" id="273116-14325324"/>
    <dbReference type="GeneID" id="1441199"/>
    <dbReference type="KEGG" id="tvo:TVG1116741"/>
    <dbReference type="eggNOG" id="arCOG01262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="OrthoDB" id="57317at2157"/>
    <dbReference type="PhylomeDB" id="Q979S4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051122">
        <property type="entry name" value="SDR_DHRS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477:SF1">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="233" mass="25311" checksum="664A3D8641B7E04B" modified="2001-10-01" version="1">MATERVLIAGTGENLGYFTALNLLKSGFKVSIIARNKEKLENMGSELRKYGDIEYFAVDLSKREGLASLKKHFSSLNYQIKGVVVEVGGYASDSLDSITAFDQMIEANLKIPILVLSAISGMLDKGSSIVFITSVFASRKIAESSVSYSLSKAGLNRLVEISAKILRQKGIRVNGISTSIISQKPHDKDKIEWQPGTQNVDPELISNVVVFLMSDLSLGVTGNIIYVDKGSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q979T4</accession>
    <name>Q979T4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fe/B12 periplasmic-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1104505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917306.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979T4"/>
    <dbReference type="STRING" id="273116.gene:9381872"/>
    <dbReference type="PaxDb" id="273116-14325314"/>
    <dbReference type="GeneID" id="1441188"/>
    <dbReference type="KEGG" id="tvo:TVG1104505"/>
    <dbReference type="eggNOG" id="arCOG04233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_2_8_2"/>
    <dbReference type="OrthoDB" id="24039at2157"/>
    <dbReference type="PhylomeDB" id="Q979T4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="256" mass="29223" checksum="0503C67A70C80AF8" modified="2001-10-01" version="1">MPRYERIVSLSPAITEILFMIGLGEYVIGDSAFCVRPDKARRIRKVGSYGTVRMEILEEIDPDVIFTISGFPHNLSTLLSEKFNVKELKLPSNVAGILNLVMEVGYETDAVEEARELYRNLVEQIGKINYKVGTVYIELDLGGPVSFGAYSYITDALYLKGLNSIYQGERREWLTPDFSYVKKMDPDIIIFEPKMFRSIDEDKIVNERGWSDLKAVKNDNVFVTPGNYDFFAHHGPSFITEVLPWIDTIIEKSKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q979V2</accession>
    <name>Q979V2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1083343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917287.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979V2"/>
    <dbReference type="STRING" id="273116.gene:9381852"/>
    <dbReference type="PaxDb" id="273116-14325296"/>
    <dbReference type="GeneID" id="1441169"/>
    <dbReference type="KEGG" id="tvo:TVG1083343"/>
    <dbReference type="eggNOG" id="arCOG00463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115699_2_0_2"/>
    <dbReference type="OrthoDB" id="17296at2157"/>
    <dbReference type="PhylomeDB" id="Q979V2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002837">
        <property type="entry name" value="DUF123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460:SF1">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01986">
        <property type="entry name" value="DUF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14446" checksum="220D5391AB3A09AC" modified="2001-10-01" version="1">MKSYIVFFLCKSQVIKLSSKTFDIDSGLYAYLGSCGKACASRISRHMIRSISKKRWHVDYLDCEPLSAIIFDCPEHEMGVALSNLEHVKSFGNSDDPNVPRLFRITDMPIFLNYVIVVCAKIKYNNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q979W9</accession>
    <name>Q979W9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1067415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979W9"/>
    <dbReference type="STRING" id="273116.gene:9381835"/>
    <dbReference type="PaxDb" id="273116-14325279"/>
    <dbReference type="KEGG" id="tvo:TVG1067415"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_695632_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q979W9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF6">
        <property type="entry name" value="TYPE IV PILUS RETRACTATION ATPASE PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="425" mass="47969" checksum="C414EE3AE082E0DC" modified="2001-10-01" version="1">MFSHHQTYHTISIMVDSDSHVTVVEPNSDQKLDTAVATVLHEISSFQSFAVERDLEDVIERISKVFRISDIDAFRYYINKSISGLGKIYPLFNLEEVKEIDVNGYDEPVIVKLSDDNYYTTNIKLLANEVDSIAIRLAQLMGKKISTSDPLARGTLNNREVSITFGTEISPKGSSIRIARNDSGYDLKKFVESNGFSLESISYLKIAIENSKSIVIIGPHTELKYSALLFLMDLVDKEKKITYIGEKIPSRSPGNYIFMKTKEKSIFSSQIDRVSLINTAMRQRPDYIVIDTIEVVDLPSVIQAITTGHPTFTSIDIDSVSALVSAFSDSALDLGRDMVSVIDIVIEISESSDRISSIYEFDRFEHNDVLYDLVFSIDKTTEKLRYNGFSPMFRSMSNAMGKDDFHRYLENVRKITSELIENGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Z5</accession>
    <name>Q979Z5_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1035393</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917243.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Z5"/>
    <dbReference type="STRING" id="273116.gene:9381808"/>
    <dbReference type="PaxDb" id="273116-14325253"/>
    <dbReference type="GeneID" id="1441125"/>
    <dbReference type="KEGG" id="tvo:TVG1035393"/>
    <dbReference type="eggNOG" id="arCOG02175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_313455_0_0_2"/>
    <dbReference type="OrthoDB" id="42357at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050545">
        <property type="entry name" value="Mycobact_MmpL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406">
        <property type="entry name" value="MEMBRANE PROTEIN MJ1562-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406:SF6">
        <property type="entry name" value="MEMBRANE PROTEIN YDGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="525"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="601"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="656"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="694"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="821"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="846"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="873"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="900"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="946"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="978"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="500"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="872"/>
            <end position="1000"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="1015" mass="112490" checksum="926691640C472D4A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Z9</accession>
    <name>ENO_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase</fullName>
            <ecNumber evidence="1">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eno</name>
        <name type="ordered locus">TV1011</name>
        <name type="ORF">TVG1033459</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of 2-phosphoglycerate (2-PG) into phosphoenolpyruvate (PEP). It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds a second Mg(2+) ion via substrate during catalysis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <text evidence="1">Fractions of enolase are present in both the cytoplasm and on the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917239.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Z9"/>
    <dbReference type="SMR" id="Q979Z9"/>
    <dbReference type="STRING" id="273116.gene:9381804"/>
    <dbReference type="PaxDb" id="273116-14325249"/>
    <dbReference type="GeneID" id="1441121"/>
    <dbReference type="KEGG" id="tvo:TVG1033459"/>
    <dbReference type="eggNOG" id="arCOG01169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_0_1_2"/>
    <dbReference type="OrthoDB" id="8680at2157"/>
    <dbReference type="PhylomeDB" id="Q979Z9"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <feature type="chain" id="PRO_0000134038" description="Enolase">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <sequence length="401" mass="43517" checksum="C14F77CA1F87016C" modified="2001-10-01" version="1">MELPIEDVRVRKVLDSRGNFTVEADVYIPGGFGRTSAPAGASTGETEVIAFSKKGIDESIKFFETNVRRSIIGFNALDQKGFDALITDLDGSGNFSNLGGNLSTALSMSVAKAVSAHLGIPLYRYVGGINHSMPRPIGNVIGGGKHARNGTSIQEFLVSAQGKTFMESAYVNVLVHRKIGDILSEKMKDISIGVGDERAWSVNISDEEAFEVLNQAVNEVSSETKVKIYTGLDFAADSLYENGKYVYKHTVRSRDEQIDYAISINKDFGVYYIEDPMFDTDFEGFAEITKKIGDKAMIVGDDLYTTNPDRIRKGIELGSTNAVLIKVNQIGTLTKAQEAASLASSAGLKNVVSHRSGETTDDFLAHLSVAFSSTFVKTGTIGGERIAKLNELMRIEECLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A06</accession>
    <name>Q97A06_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG1026247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_052268472.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A06"/>
    <dbReference type="STRING" id="273116.gene:9381797"/>
    <dbReference type="PaxDb" id="273116-14325221"/>
    <dbReference type="GeneID" id="1441114"/>
    <dbReference type="KEGG" id="tvo:TVG1026247"/>
    <dbReference type="eggNOG" id="arCOG00062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_3_1_2"/>
    <dbReference type="OrthoDB" id="41015at2157"/>
    <dbReference type="PhylomeDB" id="Q97A06"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07197">
        <property type="entry name" value="nitrilase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF2">
        <property type="entry name" value="BETA-UREIDOPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB60146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="250" mass="28754" checksum="942C23302CBA4D17" modified="2001-10-01" version="1">MNELSITAVQLHRQHFEDGLAYLEKVIDAKNSDFLVLPEKWSTEVMDQQAVIDLIKDSIVSKFNIAVPGSFSIKENGFLYNRAYIFHTGKLIGYQDKISLYGNEGRHYNSGSEIRIFRKDDLRIGIAICYDIDFPYYPKLLAKNKADIIANPSLIRSIFSDEWHLYVSARSLENRIPVLSVNSLSDPYKGKSIFSHPYEYQGGGKLRLEIFDDEAFTVTLNKKDFTELRDRRINEDPGVYSGYKVNIVDF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A09</accession>
    <name>END4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable endonuclease 4</fullName>
            <ecNumber evidence="1">3.1.21.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endodeoxyribonuclease IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Endonuclease IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nfo</name>
        <name type="ordered locus">TV1001</name>
        <name type="ORF">TVG1022523</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.21.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 3 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.2" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917229.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A09"/>
    <dbReference type="SMR" id="Q97A09"/>
    <dbReference type="STRING" id="273116.gene:9381794"/>
    <dbReference type="PaxDb" id="273116-14325218"/>
    <dbReference type="GeneID" id="1441111"/>
    <dbReference type="KEGG" id="tvo:TVG1022523"/>
    <dbReference type="eggNOG" id="arCOG01894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025885_0_4_2"/>
    <dbReference type="OrthoDB" id="33250at2157"/>
    <dbReference type="PhylomeDB" id="Q97A09"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008833">
        <property type="term" value="F:deoxyribonuclease IV (phage-T4-induced) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00729">
        <property type="entry name" value="AP_NUCLEASE_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000190895" description="Probable endonuclease 4">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00152"/>
        </source>
    </evidence>
    <sequence length="280" mass="31304" checksum="AEB9FC7693720A20" modified="2001-10-01" version="1">MDDDLKSIASKYYIGGHISVSGGLHLAPERASIFGFRTFQFFSKNQMRWNSTPISEEEALRFISEVSSHSISSTMVHASYLINLASSSNELHQKSFNAFVEEIQRAERIDATFLTFHPGSNGNKDEGIRKIKEAIEKVETHKVKLLVENTAGQGNVIGSTIYEIGQIIDGFDSSVGVCIDTCHAWAAGYDITNKYDEFIDELDSAIGLDRIYAFHLNDAMKDLGSNIDRHELIGRGKIGEGLIKLISDVRLFGKPKIMETPYGEAKFEENLKYIRKKLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A15</accession>
    <name>Q97A15_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG1019152 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG1019152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917224.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A15"/>
    <dbReference type="SMR" id="Q97A15"/>
    <dbReference type="STRING" id="273116.gene:9381787"/>
    <dbReference type="PaxDb" id="273116-14325212"/>
    <dbReference type="DNASU" id="1441106"/>
    <dbReference type="GeneID" id="1441106"/>
    <dbReference type="KEGG" id="tvo:TVG1019152"/>
    <dbReference type="eggNOG" id="arCOG00320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_896058_0_0_2"/>
    <dbReference type="OrthoDB" id="40991at2157"/>
    <dbReference type="PhylomeDB" id="Q97A15"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="10"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="301" mass="34805" checksum="2C51B124183C3486" modified="2001-10-01" version="1">MSTNLRRIQKIKGGSYIVSLPSDWVRENGLDVSREVEVFESSGIIKIRPKKDFNFERIIDFTDEETAEYLTSVYYMQGMTKITIQSKDVISRDVKDKIKMLQLELPGLNLKEESFNSITFEVDEEILKVQDSFFDFSNKALSILKDVTKIIETPREDMKQDLTSRCLALNSDYFKLIRSIALTVQRDDKYKLDVPVKDIILYAVASRDLGRFITHTRLLIGNIKQEDMKMRDQVLLLINTMENIITMFKTEDINMASRIRKNAHEILESVKSTSEANKELERMTGYALALMDDAVHKAVHL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-28" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A25</accession>
    <name>GMHA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable phosphoheptose isomerase</fullName>
            <ecNumber evidence="1">5.3.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Sedoheptulose 7-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gmhA</name>
        <name type="ordered locus">TV0985</name>
        <name type="ORF">TVG1006486</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 D-sedoheptulose 7-phosphate = D-glycero-alpha-D-manno-heptose 7-phosphate + D-glycero-beta-D-manno-heptose 7-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27489"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:60203"/>
            <dbReference type="ChEBI" id="CHEBI:60204"/>
            <dbReference type="EC" id="5.3.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIS family. GmhA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.28" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A25"/>
    <dbReference type="SMR" id="Q97A25"/>
    <dbReference type="STRING" id="273116.gene:9381777"/>
    <dbReference type="PaxDb" id="273116-14325202"/>
    <dbReference type="KEGG" id="tvo:TVG1006486"/>
    <dbReference type="eggNOG" id="arCOG05355">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080999_3_1_2"/>
    <dbReference type="PhylomeDB" id="Q97A25"/>
    <dbReference type="UniPathway" id="UPA00041">
        <property type="reaction ID" value="UER00436"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008968">
        <property type="term" value="F:D-sedoheptulose 7-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001061">
        <property type="term" value="P:D-glycero-D-manno-heptose 7-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05006">
        <property type="entry name" value="SIS_GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00067">
        <property type="entry name" value="GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035461">
        <property type="entry name" value="GmhA/DiaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004515">
        <property type="entry name" value="Phosphoheptose_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050099">
        <property type="entry name" value="SIS_GmhA/DiaA_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30390:SF6">
        <property type="entry name" value="DNAA INITIATOR-ASSOCIATING PROTEIN DIAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30390">
        <property type="entry name" value="SEDOHEPTULOSE 7-PHOSPHATE ISOMERASE / DNAA INITIATOR-ASSOCIATING FACTOR FOR REPLICATION INITIATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13580">
        <property type="entry name" value="SIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000136554" description="Probable phosphoheptose isomerase">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="1">
        <location>
            <begin position="43"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="100"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00067"/>
        </source>
    </evidence>
    <sequence length="196" mass="21178" checksum="80F09B2268673734" modified="2001-10-01" version="1">MGRKDKYALIHYKLMDLSDYIEAGNKARRSIDLNHIEKIGRDIVNVFNSGGKLIVFGNGGSAADSQHFVAELSGHFSKERKALPAMALTVNTSALTAISNDYSYDVVFSRQLEAFAKPGDYVVGISTSGNSVNVVKGLERAKELGCKTLAMTGRSGGKIAKVAEEAIMIDSEVTSIIQEAHIAAIHMICSVIDSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A26</accession>
    <name>Q97A26_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein-L-isoaspartate methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1006378</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_083755846.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A26"/>
    <dbReference type="STRING" id="273116.gene:9381776"/>
    <dbReference type="PaxDb" id="273116-14325201"/>
    <dbReference type="GeneID" id="1442061"/>
    <dbReference type="KEGG" id="tvo:TVG1006378"/>
    <dbReference type="eggNOG" id="arCOG00978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025402_0_1_2"/>
    <dbReference type="OrthoDB" id="30774at2157"/>
    <dbReference type="PhylomeDB" id="Q97A26"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF1">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="6">
        <location>
            <begin position="81"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="115"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="271" mass="30419" checksum="F062BA0D6AF5364F" modified="2001-10-01" version="1">MAYPLIDQAYRNISLAIVICTFMILTSSDEDCTLENKTVNCKNKKIFIPPNFVASPGDLISVDGQPFIVAKFMPFNYGRLIKRRTQIISPADAAYIIMRTGLGIGMKAMEIGVGSGNLSAYILWAIGKEGHLTLVDREMESIQFSLNNLSKFYDLENVDYVIGDPSSLKVNNDYDALFVDIPNPWNYLENLKMVLKPGSMAEFYLPNFDQVEKTVLGLEEHGFVHLETAEIQRRRLIVRKDATRPASDDLTHSAYMVFSIFTSGLKASLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A31</accession>
    <name>Q97A31_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG1000457 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG1000457</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917208.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A31"/>
    <dbReference type="STRING" id="273116.gene:9381771"/>
    <dbReference type="PaxDb" id="273116-14325196"/>
    <dbReference type="DNASU" id="1442056"/>
    <dbReference type="GeneID" id="1442056"/>
    <dbReference type="KEGG" id="tvo:TVG1000457"/>
    <dbReference type="eggNOG" id="arCOG05361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052486_1_1_2"/>
    <dbReference type="OrthoDB" id="39584at2157"/>
    <dbReference type="PhylomeDB" id="Q97A31"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01081">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF0">
        <property type="entry name" value="GALACTOSE MUTAROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="270" mass="30702" checksum="61496FB53454C319" modified="2001-10-01" version="1">MDISIGDDSNGAVISIVGSHLDKLNLNGTAILLERYDDSPTHFGSAFLFPYANRVRNGEFTLDGKRYFLPKDDENNSIHGLVLSKKFDLVEKIENSVTMRYIMADECYPSPIEIFITHTVSEGKYTCRVKASNRGLTRAPVSAGFHPYFLYNGRWAIKKPRDTWKLEYDGQFPTGKMDKFLFEEDSFMHEFDNQFFADEDIVVDMGSHMIQIERENMPFFVIYNGKYSGGKSVAIEPMMSAVDAFNNGIGLRIVNPGESIEFAYSVEIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A41</accession>
    <name>Q97A41_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0993143 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0993143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_156769099.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A41"/>
    <dbReference type="STRING" id="273116.gene:9381761"/>
    <dbReference type="PaxDb" id="273116-14325186"/>
    <dbReference type="GeneID" id="1442047"/>
    <dbReference type="KEGG" id="tvo:TVG0993143"/>
    <dbReference type="eggNOG" id="arCOG07394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302690_0_0_2"/>
    <dbReference type="OrthoDB" id="376251at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="211" mass="23561" checksum="FAD77F570EEC3E0B" modified="2001-10-01" version="1">MRQTLSFLILIFAVFASSMVVTTGIALQEQGMTSVAIVGPLTIKNIENVDSHYYLNVRIALVNRAYTVSEVTFYHDEVIVPALTSVQANVLIPLDMNTLLKSWPTHSVNITIPFEEHVFFVIFQKHIDLKLPKLFDNFTIEQNGSNMVLTVKPSNFTSGNTITVYANGNQKSGQLELGRSWSYEFTIVSNDSSGYAGIAISISNFTFYYVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-23" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A55</accession>
    <name>ASSY_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate synthase</fullName>
            <ecNumber evidence="1">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argG</name>
        <name type="ordered locus">TV0955</name>
        <name type="ORF">TVG0979194</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-citrulline + L-aspartate + ATP = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous termination">
            <sequence resource="EMBL-CDS" id="BAB60097" version="1"/>
        </conflict>
        <text>Truncated C-terminus.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60097.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A55"/>
    <dbReference type="SMR" id="Q97A55"/>
    <dbReference type="STRING" id="273116.gene:9381747"/>
    <dbReference type="PaxDb" id="273116-14325172"/>
    <dbReference type="KEGG" id="tvo:TVG0979194"/>
    <dbReference type="eggNOG" id="arCOG00112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032784_1_0_2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword id="KW-0547">Nucleotide-binding</keyword>
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        </ligand>
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            <position position="83"/>
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        <ligand>
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        <ligand>
            <name>L-aspartate</name>
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        </ligand>
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            <position position="123"/>
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        </ligand>
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        <location>
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        </ligand>
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        <source>
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        </source>
    </evidence>
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    <sequence length="397" mass="44342" checksum="2A0DBCDF55AD880F" modified="2007-02-20" version="3">MKELLLLYSGGLDTSVMIKWMNENLGYDVSTLTLDIGNNDLKSIREKAEAIGAVETFVEDVKQEFSYEYISKSILANGSYEGYPLSTALARPLMAKKAVELAEKHGFEAIAHGSTGRGNDQVRFELGIKALNPSLEMLAPVRDWNMLRSEEIEYAKKNNIIVPSDGKYSVDENLWGRSIEGSEIENMSLPVPEDAYEWLVPPWEAGPGEVIKLEFSNGLPVAINDSDFELQNLIANLNIIGGRNSIGLIDHVEDRITGIKSREVYECPAAEIITYAHGYLESLILNKHEISIKSFLDSKFSQFVYNGLWYDPAMKPLIAGEEILNSEINGSISLKLYKGKIYFNGSKGANFSYNSNVANYSTYEFDQKSSKGFIDIFKNDTVYSILARQKAKEEALS</sequence>
</entry>
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    <name>Q97A56_THEVO</name>
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            <taxon>Thermoplasmata</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
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                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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            <end position="60"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="61" mass="6765" checksum="3917EC2720D1C5AC" modified="2001-10-01" version="1">MKELLLLYSGGLDTSVMIKWMNENLGYDVSTLTLDIGNNDLKSIREKAEAIGAVETFVEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A68</accession>
    <name>Q97A68_THEVO</name>
    <protein>
        <submittedName>
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    <organism evidence="7 8">
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
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                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
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                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="1442022"/>
    <dbReference type="KEGG" id="tvo:TVG0968891"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028397_1_0_2"/>
    <dbReference type="OrthoDB" id="4722at2157"/>
    <dbReference type="PhylomeDB" id="Q97A68"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:cobalamin biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
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        <property type="entry name" value="CobE/GbiG C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
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        <property type="entry name" value="4pyrrol_Mease"/>
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    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
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    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052553">
        <property type="entry name" value="CbiG_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021744">
        <property type="entry name" value="CbiG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02467">
        <property type="entry name" value="CbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11760">
        <property type="entry name" value="CbiG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="CbiG N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="CobE/GbiG C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cobalamin synthesis G N-terminal" evidence="6">
        <location>
            <begin position="66"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="CobE/GbiG C-terminal" evidence="5">
        <location>
            <begin position="228"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="4">
        <location>
            <begin position="356"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="574" mass="63823" checksum="0C05AFFCCA6719B4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A97</accession>
    <name>Q97A97_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gamma-glutamyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0939251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A97"/>
    <dbReference type="STRING" id="273116.gene:9381705"/>
    <dbReference type="MEROPS" id="T03.025"/>
    <dbReference type="PaxDb" id="273116-14325130"/>
    <dbReference type="KEGG" id="tvo:TVG0939251"/>
    <dbReference type="eggNOG" id="arCOG04053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_3_1_2"/>
    <dbReference type="PhylomeDB" id="Q97A97"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052896">
        <property type="entry name" value="GGT-like_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881:SF1">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="505" mass="55719" checksum="A268572BC473B8BE" modified="2001-10-01" version="1">MMRPTVFSSKAVISSSSLSASLAGAKIIEKGGNIFDAAIAVSSVLSVTQNNLCGLGGDAFALVRYNGRVKGYNGSGKSSRNVNRGLYFELGYSRIPERGIYSAITVPGLVDLWNSLYLEYATMRFSDLISDAIKYAETGFYITRHYEESLIASSPILGQYNWSNQFMPYGKIPAEGDLFIQKDLAGTLREIAEEGPRSFYDGYLSDIIFKAMEKTNLLIDSKDLSDHKTLAANPVKSTYMDYDVFETAPNSQGATVNYWLALLEQKIQNKIPRPVDIVESGLEAYRWRSMFIGDPERMPFNGDLLSKGAGPYYNNPRVPEDKGDTTYFSISDANGNSISMIQSNYMGFGSGIIPEGTGFVLQNRGSYFTLDDKHHNSLEPRKRTFHTLAACIIEKDGELVYSLGTMGGDIQPQVQVQIITGLINQDLMPQEAIERPRWAFPATIYEQPSVLNVEDTSLINENETRLRINPMKYSSSFGTAQVTALLNGRVAGGSDPRGDGFSIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AA9</accession>
    <name>Q97AA9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0922512 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0922512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AA9"/>
    <dbReference type="PaxDb" id="273116-14325118"/>
    <dbReference type="KEGG" id="tvo:TVG0922512"/>
    <dbReference type="HOGENOM" id="CLU_1976654_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB60043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="126" mass="14917" checksum="7599A9D571E15DBC" modified="2001-10-01" version="1">MKRIMETYLRRSDIEVMNRDIKQDGLGHIFMRKLCKTELYLRLMVTGRVILEIAAIMSMRKYLYAHKRIGKRKRWIGYEFLESLLDGFSKYGDRFVMAVKQSKLNPYKSAGHVMKTRDKLNHGVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AD2</accession>
    <name>Q97AD2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0895451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917122.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AD2"/>
    <dbReference type="STRING" id="273116.gene:9381670"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14325095"/>
    <dbReference type="DNASU" id="1441970"/>
    <dbReference type="GeneID" id="1441970"/>
    <dbReference type="KEGG" id="tvo:TVG0895451"/>
    <dbReference type="eggNOG" id="arCOG01419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_753599_0_0_2"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="250"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="367" mass="42114" checksum="17BD2C3217372E37" modified="2001-10-01" version="1">MKIAIVVPYVHKIEGNRLAFSLARALSRLNSVTLFIHTITQRTKESVEDYIRPAELKYIKIIDQGHFGVRFSLNYQFLRKKAKELAKFITDNGEYDIVFVIANEGHWLPQYIKKNSRAKVLLLVMELHDHGVVSMERYPEKNSMLRNIVFSPFYGFLKFFERSRFEEFDGFFANSTWTKTLFEYLYGLYVQDVLYVIDTDLFKPGEPYTADKYIAVPTVSLSSDINGREMVKRLAKDGIPLVAYGPLNLEGVKNLGYLDENTMVKVLSGASATLFLYNYEALGLIPFESLACGTPVITYNKQGPSLELAGNENVTFISTYEELLDACRKALEREKDNEIKESCRTSVIRFSPENVGERLEKDLQKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AD9</accession>
    <name>Q97AD9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0890000 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0890000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917115.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AD9"/>
    <dbReference type="STRING" id="273116.gene:9381663"/>
    <dbReference type="PaxDb" id="273116-14325088"/>
    <dbReference type="GeneID" id="1441963"/>
    <dbReference type="KEGG" id="tvo:TVG0890000"/>
    <dbReference type="eggNOG" id="arCOG11046">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079537_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB60013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="120" mass="14230" checksum="89FF80290EDEBB1A" modified="2001-10-01" version="1">MSYRDRILFILLFYIVLIVLVDFLFPIFLPAYAGAFSYFPLIFFFPFFMFGRRYRGRQAPAPAEDESSDEKKDDYDYAYQYENYDEFGIRKRRRDSIIFYALGAAILIAAIAVVLLKFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AE8</accession>
    <name>Q97AE8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG0882679</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AE8"/>
    <dbReference type="STRING" id="273116.gene:9381654"/>
    <dbReference type="PaxDb" id="273116-14325079"/>
    <dbReference type="KEGG" id="tvo:TVG0882679"/>
    <dbReference type="eggNOG" id="arCOG01130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="PhylomeDB" id="Q97AE8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="39"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="390" mass="43854" checksum="7606C67A95B53F70" modified="2001-10-01" version="1">MNCRMKQSTIGRNIVDEISFGAIVKIRDQLLEMQRQGKKVYRLESGDPSFSLPPHVKEAIKQAIENNKTHYTDSTGIPELRKAIAEKLVRKNKIKDATPENVIVSNGGMNALYVTFRSLLSPGDEVIIPDPMWTEIAEIIKLAEGVPIRLPVENYIEEMQKYEDDDKVKAVFVNSPHNPTGLVFTPKQIDGIISFAESKGIFIVSDEAYEDVIFDGREHVSPGSKYDNTISLFSMSKTYAMSGLRIGYAHTNSEILYDRMKKLLRCTINGVNSATQYGAVAALTGPQEFVGQMRSEYQKRRDIIFNAVSKSRYLEPVKPGGTFYLWAKIKEYPKEVKDSWDMTYYLLDKTGVGSSPGPVFGPAGDGYIRFAFSADTEHVREASELIEKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AK5</accession>
    <name>Q97AK5_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorite dismutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0805714</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917049.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AK5"/>
    <dbReference type="STRING" id="273116.gene:9381595"/>
    <dbReference type="PaxDb" id="273116-14325021"/>
    <dbReference type="GeneID" id="1441897"/>
    <dbReference type="KEGG" id="tvo:TVG0805714"/>
    <dbReference type="eggNOG" id="arCOG03031">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076582_0_0_2"/>
    <dbReference type="OrthoDB" id="8690at2157"/>
    <dbReference type="PhylomeDB" id="Q97AK5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1030">
        <property type="entry name" value="Apc35880, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010644">
        <property type="entry name" value="ChdC/CLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36843:SF1">
        <property type="entry name" value="COPROHEME DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36843">
        <property type="entry name" value="HEME-DEPENDENT PEROXIDASE YWFI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06778">
        <property type="entry name" value="Chlor_dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="26102" checksum="6C6C7E5A1FC3A23F" modified="2001-10-01" version="1">MNEVYTFVTAYKFLNGYVKDIGSVKEPLNKLSSILRDAKSKMINLHTYRSFRYDSDLVFWFSSRTPDDILELKEKIGFTLLPYANATYSSISIYDESPYNAMNKKLEDSLKLKPLDYFVAYPMSKDPEWYLLPFDERKEIMREHIDTALKHPDEKGIRSYTTYSFGIGDQEFVVLYELPDIAAWSRVTEKLREVKARKWIVKETPIILGRLIDLNTLSDGFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AN3</accession>
    <name>Q97AN3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0780828</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917022.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AN3"/>
    <dbReference type="STRING" id="273116.gene:9381567"/>
    <dbReference type="PaxDb" id="273116-14324993"/>
    <dbReference type="GeneID" id="1441870"/>
    <dbReference type="KEGG" id="tvo:TVG0780828"/>
    <dbReference type="eggNOG" id="arCOG00683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_16_0_2"/>
    <dbReference type="OrthoDB" id="142001at2157"/>
    <dbReference type="PhylomeDB" id="Q97AN3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="5">
        <location>
            <begin position="172"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="6">
        <location>
            <begin position="310"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="48470" checksum="240B8AFBAC0313B4" modified="2001-10-01" version="1">MKVRRTEQIYLKENDTISYMCHLSKNLYNQVNYILRQQFLKGEKLTGYRDLVKLFQEPSEIEDHNNYQRLPAQTAQWTVKKTVMAWYSFFKSMKAWKKHPDKFKGRPRLPGYKETDGEFLLVFTNQQCRIRDGILKFPKIMNMEVKTRLNENEIKLKEVRIVPQGTGYMIEIVYEKETADIPITKPKRVMGIDIGVRNLVTIGNNIHERGIAVKAGLLKSINQYFNKELARYRSINDLQGNERKQTVKIRKLFMKRNRKVKDIMHKVSKSIVEYAKIMNIDTIVIGHNNRWKDSSNMGKKNNQNFVQLPFNMLISQIKYKAEEIGITVIMQNEDYTSKCSFLDNESIEHHGTYMGKRIHRGIFQSATGKLIHADLQSSYNIIKKAIPEAFANGIEGIGLYPRSLSIRQMITSKGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AW0</accession>
    <name>Q97AW0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0703837 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0703837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AW0"/>
    <dbReference type="STRING" id="273116.gene:9381487"/>
    <dbReference type="PaxDb" id="273116-14324915"/>
    <dbReference type="KEGG" id="tvo:TVG0703837"/>
    <dbReference type="eggNOG" id="arCOG06915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_741072_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="373" mass="42522" checksum="B74F6A87E585066E" modified="2001-10-01" version="1">MPNRENELRAFYRRIIAIIDFPNRLEDFLTPAKISQIVEKMKNPDELDAIFSYVVDNYYAPLAQRMKFTGQLDLKEAQGMWEERSNPAMAYLRRRNEAGEILTNIEDARVTIMEAGKDSRRYISREKDSMEEYLATPKQEVIQDAVKWATEKGFPAKNINAGTLGKELNALGYPNLTVNKKIVKSAPVKAWRDLLIITWTPVADQKNGTIPQENQSEMRASAMRNGSGFYPEDMWKQNVSDHEGNRQASVTHEQSTLENSEQKTVAGTSGEPLLNRYSKNSESQPDPVNSGVPDEKTDKPKNQQFTFRKIITSDPRLNPEPERLKEGTYVAMTISEARQIKAYNFSSEASEREFRTLADDDQKKAAIEKGWSP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AY8</accession>
    <name>Y671_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0145 protein TV0671</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0671</name>
        <name type="ORF">TVG0668267</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0145 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916931.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AY8"/>
    <dbReference type="SMR" id="Q97AY8"/>
    <dbReference type="PaxDb" id="273116-14324887"/>
    <dbReference type="GeneID" id="1441779"/>
    <dbReference type="KEGG" id="tvo:TVG0668267"/>
    <dbReference type="eggNOG" id="arCOG02287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117144_1_1_2"/>
    <dbReference type="OrthoDB" id="59443at2157"/>
    <dbReference type="PhylomeDB" id="Q97AY8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.70">
        <property type="entry name" value="Hypothetical protein apc22750. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00338">
        <property type="entry name" value="UPF0145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145_YbjQ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068:SF2">
        <property type="entry name" value="UPF0145 PROTEIN SCO3412"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="UPF0145 PROTEIN YBJQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="YbjQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138506" description="UPF0145 protein TV0671">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00338"/>
        </source>
    </evidence>
    <sequence length="122" mass="13213" checksum="0B04FBEFC80DE45E" modified="2001-10-01" version="1">MDEEHNIIMVTTNYIPGKKITRIIGTIWGITVRSRGLGGNIVAGLRSLAGGEIKEYSKMLSDTRNTAMERLRDAAEQVGANAVIELRFDSSDIGQVMTEIVAYGTAVVVEDVSSDIQRVGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AZ6</accession>
    <name>Q97AZ6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription termination factor FttA</fullName>
            <ecNumber evidence="12">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fttA</name>
        <name evidence="15" type="ORF">TVG0658611</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Terminates transcription on the whole genome. Termination is linked to FttA-mediated RNA cleavage and does not require NTP hydrolysis. Cleaves endonucleolytically at the RNA exit channel of RNA polymerase (RNAP); the 5'-3' exonuclease activity of this protein degrades the nascent RNA released from RNAP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 2 Zn(2+) ions, which are required for nuclease activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with RNA polymerase (RNAP), interacts with the Spt4-Spt5 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. FttA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="12"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916922.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AZ6"/>
    <dbReference type="STRING" id="273116.gene:9381451"/>
    <dbReference type="PaxDb" id="273116-14324879"/>
    <dbReference type="GeneID" id="1441770"/>
    <dbReference type="KEGG" id="tvo:TVG0658611"/>
    <dbReference type="eggNOG" id="arCOG00543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="OrthoDB" id="7155at2157"/>
    <dbReference type="PhylomeDB" id="Q97AZ6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004532">
        <property type="term" value="F:RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22532">
        <property type="entry name" value="KH-II_CPSF_arch_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00870">
        <property type="entry name" value="FttA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019975">
        <property type="entry name" value="aCPSF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033769">
        <property type="entry name" value="TffA_KH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03675">
        <property type="entry name" value="arCOG00543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF51">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17214">
        <property type="entry name" value="KH_TffA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 12" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="13">
        <location>
            <begin position="192"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="14">
        <location>
            <begin position="424"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="KHb" evidence="12">
        <location>
            <begin position="71"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Beta-Casp" evidence="12">
        <location>
            <begin position="387"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Metallo-beta-lactamase C-terminus" evidence="12">
        <location>
            <begin position="581"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="606"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAB59805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="639" mass="72332" checksum="E9114865B7009AC3" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B08</accession>
    <name>Q97B08_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0645988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B08"/>
    <dbReference type="STRING" id="273116.gene:9381439"/>
    <dbReference type="PaxDb" id="273116-14324867"/>
    <dbReference type="KEGG" id="tvo:TVG0645988"/>
    <dbReference type="eggNOG" id="arCOG05403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182726_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="71" mass="8364" checksum="31516816253D87A9" modified="2001-10-01" version="1">MLKTSSSGIKDVKLYQDVNDKQQYMIFTEWESLESFKNFIASRPFKETTDYGKSILEDTPKNRIFMNETSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B30</accession>
    <name>Q97B30_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protoporphyrin IX magnesium chelatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0623447</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916888.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B30"/>
    <dbReference type="STRING" id="273116.gene:9381417"/>
    <dbReference type="PaxDb" id="273116-14324845"/>
    <dbReference type="GeneID" id="1441736"/>
    <dbReference type="KEGG" id="tvo:TVG0623447"/>
    <dbReference type="eggNOG" id="arCOG00438">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016684_6_2_2"/>
    <dbReference type="OrthoDB" id="25914at2157"/>
    <dbReference type="PhylomeDB" id="Q97B30"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039:SF9">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLI-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="29"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="5">
        <location>
            <begin position="437"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="338"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="357"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="614" mass="68411" checksum="AA8AC02CF6867E5A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B34</accession>
    <name>Y625_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0147 protein TV0625</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0625</name>
        <name type="ORF">TVG0617710</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0147 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B34"/>
    <dbReference type="SMR" id="Q97B34"/>
    <dbReference type="STRING" id="273116.gene:9381413"/>
    <dbReference type="PaxDb" id="273116-14324841"/>
    <dbReference type="KEGG" id="tvo:TVG0617710"/>
    <dbReference type="eggNOG" id="arCOG04308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165882_1_0_2"/>
    <dbReference type="OrthoDB" id="65304at2157"/>
    <dbReference type="PhylomeDB" id="Q97B34"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.50">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00342">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023130">
        <property type="entry name" value="Ta0600-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005354">
        <property type="entry name" value="UPF0147"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003319">
        <property type="entry name" value="PRK04330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03685">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158436">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150920" description="UPF0147 protein TV0625">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00342"/>
        </source>
    </evidence>
    <sequence length="89" mass="10005" checksum="F7EB6C12B648B5FB" modified="2001-10-01" version="1">MVMAMDQNLFNEVMYLLDELSQDTTVPKNVRKVAQDSKTKLSQENESLDLRCATVLSMLDEMANDPNVPSHGRTDLYTIISKLEALAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B49</accession>
    <name>Q97B49_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0600187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916868.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B49"/>
    <dbReference type="STRING" id="273116.gene:9381398"/>
    <dbReference type="PaxDb" id="273116-14324825"/>
    <dbReference type="GeneID" id="1441716"/>
    <dbReference type="KEGG" id="tvo:TVG0600187"/>
    <dbReference type="eggNOG" id="arCOG01824">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860801_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="137" mass="15331" checksum="C1FBFB261CFA1050" modified="2001-10-01" version="1">MGFSYTVAAVIMLSSTLIFFGIVYSSYVQSNTSITNANQKLIKNIYNLENTKIEIVSYYYNSSSSTFYVNVTDNGSTSFNLSESNVILNGTLISFNESNQYIFPLETITISFKASPGIYSFELVMPDGYEVFKEVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B55</accession>
    <name>Q97B55_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH marR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0594797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916861.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B55"/>
    <dbReference type="STRING" id="273116.gene:9381391"/>
    <dbReference type="PaxDb" id="273116-14324818"/>
    <dbReference type="GeneID" id="1441709"/>
    <dbReference type="KEGG" id="tvo:TVG0594797"/>
    <dbReference type="eggNOG" id="arCOG01804">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053800_0_0_2"/>
    <dbReference type="OrthoDB" id="10037at2157"/>
    <dbReference type="PhylomeDB" id="Q97B55"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014466">
        <property type="entry name" value="Txn_rg_UCP012876"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012876">
        <property type="entry name" value="Txn_rg_UCP012876"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="327" mass="37514" checksum="5BEC233262630B0A" modified="2001-10-01" version="1">MSLRDVLLRELKRARGLGIDQFQIEEMMGFSKSTVSEALKDLEEKGIIKRRTIGGKIKHVWLAEYYPYYDEKLLRIGCLRSTEYVKFVSVAKNYSESKGIDLIVRFYDDAISLMNDLSQGLLEFALAPIFTEIIYSISNRNSIILGAVASGGSGVYKNVNCNCGKILTSESSSMMILAREFAKTRKISEIAIFKDPRRAIKDYESFKAEYIVIWEPYASKIMGEKMASFSDVLDSFPCCGISVSENNRENRTLIEIVRLYKRSRFTAREDVIDIFAKSTSVSPKTAKASLENYIWDIKFYRSDLKKYLEFIGYPPIEEIIEKIYEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B62</accession>
    <name>Q97B62_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycolate oxidase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0585220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916854.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B62"/>
    <dbReference type="STRING" id="273116.gene:9381384"/>
    <dbReference type="PaxDb" id="273116-14324811"/>
    <dbReference type="DNASU" id="1441702"/>
    <dbReference type="GeneID" id="1441702"/>
    <dbReference type="KEGG" id="tvo:TVG0585220"/>
    <dbReference type="eggNOG" id="arCOG00337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_9_2_2"/>
    <dbReference type="OrthoDB" id="26910at2157"/>
    <dbReference type="PhylomeDB" id="Q97B62"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051914">
        <property type="entry name" value="FAD-linked_OxidoTrans_Type4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42934:SF3">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42934">
        <property type="entry name" value="GLYCOLATE OXIDASE SUBUNIT GLCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="33"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="459" mass="50461" checksum="A08AA90A2CB2ABD5" modified="2001-10-01" version="1">MGFIEEIKSALGNYVITEKKDLIPYMNDASYFTGEMPEAVVLPGSAEDVQTIMRLAYKYEKPVVVKGGGSSLTGSSILKESGIVMSMLRMNRILDINLNDKCVTVEPGLRLNDLQLYLDKYGHFYPPDPASSMAATVGGSISTNAGGLRGVAYGVTKDWILGLDVVLADGTMVKFGNKTLKRTLGYDLTSLMIGSEGTLGVITKAYLKIWPKPQKVARLVAFYQDIESAGRAVNELKVSTIPEMAEFLDKISLDSVPEAITYPPGTKYALIIDVSGPPELLGKKKTETEELLKRYAISTKSTTDPAEMEKIYRARKGLYASILKLRKSEKEYIVIGDIVVPVSELPTSLKEIEKKRDEDGVRAAIFGHIGDGNIHANIFADLGDNSAMDKVNRFMMDLAMVAVKHGGSVSAEHGIGIEKKQLLIEEMKYNNNLRTLDIMKAIKQVFDPKNILNRGNIFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-03" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B68</accession>
    <name>HEM1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="1">GluTR</shortName>
            <ecNumber evidence="1">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemA</name>
        <name type="ordered locus">TV0590</name>
        <name type="ORF">TVG0579613</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate + tRNA(Glu) + NADP(+) = L-glutamyl-tRNA(Glu) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="3OJ0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.65 A"/>
        <property type="chains" value="A=141-281"/>
    </dbReference>
    <dbReference type="PDBsum" id="3OJ0"/>
    <dbReference type="AlphaFoldDB" id="Q97B68"/>
    <dbReference type="SMR" id="Q97B68"/>
    <dbReference type="STRING" id="273116.gene:9381378"/>
    <dbReference type="PaxDb" id="273116-14324805"/>
    <dbReference type="DNASU" id="1441696"/>
    <dbReference type="KEGG" id="tvo:TVG0579613"/>
    <dbReference type="eggNOG" id="arCOG01036">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035113_2_2_2"/>
    <dbReference type="PhylomeDB" id="Q97B68"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97B68"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019353">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process from glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="entry name" value="Glutamyl-tRNA reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013:SF1">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="Glutamyl tRNA-reductase catalytic, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="Glutamyl tRNA-reductase dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000114113" description="Glutamyl-tRNA reductase">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
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        <ligand>
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        <ligand>
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        <location>
            <begin position="165"/>
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        </location>
        <ligand>
            <name>NADP(+)</name>
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        </ligand>
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    <feature type="site" description="Important for activity" evidence="1">
        <location>
            <position position="79"/>
        </location>
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        <location>
            <begin position="145"/>
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        </location>
    </feature>
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    <feature type="helix" evidence="2">
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        </location>
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        </location>
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            <begin position="183"/>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
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    <feature type="strand" evidence="2">
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    <feature type="strand" evidence="2">
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3OJ0"/>
        </source>
    </evidence>
    <sequence length="409" mass="46691" checksum="8CF23510B233D830" modified="2001-10-01" version="1">MLMNIVTLISWDFKRNNESFQRAIINGYDYWSRILDDHGVEKYVILLTCNRVELYYRGDPFDVQEKGYNIISDDMAINHLFHVAAGLDSMSIGENEVLKQVKQAYEKSSSMGKSDKFLSLIFQRAISVGKLVRSKTGIGKGKVSIPSIVYDIVRKNGGNKILLVGNGMLASEIAPYFSYPQYKVTVAGRNIDHVRAFAEKYEYEYVLINDIDSLIKNNDVIITATSSKTPIVEERSLMPGKLFIDLGNPPNIERGNNVITLDEIYEISKKNEMLREEKINQAEILIENEMKATMNKIKDLMIDDIFSQFYRFASVVQTMEIQKFRKMHPEVNENDLEALAHSIINKILNVPVTTLKAVSRSQGNSDFNRLFESFSSNFNDIVSAALQSYEGLRDTQSLRDRTRQLLQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
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    <name>Q97B71_THEVO</name>
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        <lineage>
            <taxon>Archaea</taxon>
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    <reference evidence="1 2" key="1">
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
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                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B71"/>
    <dbReference type="PaxDb" id="273116-14324801"/>
    <dbReference type="KEGG" id="tvo:TVG0574635"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="BAB59728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="59" mass="7259" checksum="B0623098A20A3C73" modified="2001-10-01" version="1">MSKVLPQSCIMKRKYRNDKWDKKLAFKYIIRSRENIEMMYLFINGENKIYNKMSQGYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B84</accession>
    <name>Q97B84_THEVO</name>
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            <fullName evidence="3">THUMP-like domain-containing protein</fullName>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
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                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
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                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
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                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
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            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="43010" checksum="F7819A74D841EAE1" modified="2001-10-01" version="1">MSKHLEKILERAVYDEAFRNKLNSQIEYAIQQIDRGVSVPDSVVSEFARARIRVRKKYSKWDKLFFDKYSAMYSTPENIGRYRASRINGPVLDVGSGAGMQCIMFGLEYGCKGIEVDRSRYLMSLLNSIAYRCKSEFVNSDFYKNSIRDAHIVFSDPLRTKASSISELKPSPLDIMEKIQAEAFIFDLPPRMPASTINIDGEREYISVDGVLSRFTVYTGSLKEAEYSAHIMPSGIHLNGEKQTVKFEEGVPGRLIYVPDPAVVAAGLLGAYSSLVMVSNDGRRLILSSDDTVENFPGTIYEIIHSCDGTSLAFSLRSLRPRNVYLRFSIDPVQYYSITNSVTSADGLTDLYVFRYKELFLITKKIFDINSTGNKAVNFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BA3</accession>
    <name>Q97BA3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dinitrogenase iron-molybdenum cofactor biosynthesis domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0542914</name>
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    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916812.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BA3"/>
    <dbReference type="STRING" id="273116.gene:9381339"/>
    <dbReference type="PaxDb" id="273116-14324769"/>
    <dbReference type="GeneID" id="1441070"/>
    <dbReference type="KEGG" id="tvo:TVG0542914"/>
    <dbReference type="eggNOG" id="arCOG02734">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021615_0_0_2"/>
    <dbReference type="OrthoDB" id="25911at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="entry name" value="Dinitrogenase iron-molybdenum cofactor biosynthesis domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051840">
        <property type="entry name" value="NifX/NifY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937:SF2">
        <property type="entry name" value="DINITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937">
        <property type="entry name" value="IRON-MOLYBDENUM PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dinitrogenase iron-molybdenum cofactor biosynthesis" evidence="1">
        <location>
            <begin position="16"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="119" mass="13151" checksum="9D16D558C7DF382D" modified="2001-10-01" version="1">MKIAVAVSDNKVGGPGESQYVYIYDVKDGNADLLEKYENPAMHAYRAKGLQMLASAISRGVDAIILSELGRPGYSYLKDRIKVYITPEVEVERAIKDVINGRIPVATAPTHEHGMHEHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BA6</accession>
    <name>Q97BA6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="6">FGAM synthase</shortName>
            <ecNumber evidence="6">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="6">FGAR amidotransferase III</shortName>
            <shortName evidence="6">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">purS</name>
        <name evidence="7" type="ORF">TVG0540138</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PurS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="6"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916809.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BA6"/>
    <dbReference type="STRING" id="273116.gene:9381336"/>
    <dbReference type="PaxDb" id="273116-14324766"/>
    <dbReference type="GeneID" id="1441067"/>
    <dbReference type="KEGG" id="tvo:TVG0540138"/>
    <dbReference type="eggNOG" id="arCOG04462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164833_3_1_2"/>
    <dbReference type="OrthoDB" id="56303at2157"/>
    <dbReference type="PhylomeDB" id="Q97BA6"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00302">
        <property type="entry name" value="phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005019">
        <property type="entry name" value="PRK06423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="74" mass="8371" checksum="1F0D54DB45DD0404" modified="2001-10-01" version="1">MKFKVEVSYKPGVEDPEALTLLKNLNILGYHEISNVSISKVYYFDAGNEEEVKQVADKILSNPVIHSYKVEKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BA8</accession>
    <name>Q97BA8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0538469 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0538469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916807.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BA8"/>
    <dbReference type="STRING" id="273116.gene:9381334"/>
    <dbReference type="ESTHER" id="thevo-TV0549">
        <property type="family name" value="A85-Feruloyl-Esterase"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324764"/>
    <dbReference type="DNASU" id="1441065"/>
    <dbReference type="GeneID" id="1441065"/>
    <dbReference type="KEGG" id="tvo:TVG0538469"/>
    <dbReference type="eggNOG" id="arCOG05356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037618_0_1_2"/>
    <dbReference type="OrthoDB" id="39489at2157"/>
    <dbReference type="PhylomeDB" id="Q97BA8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="320" mass="36681" checksum="F58202823C17FA31" modified="2001-10-01" version="1">MLNITEDEIDSISLLNNPLKDPSKRSIIIIDHSRNDRSPILIGLQGFFGTNRSFMNRYFNKVDFISVLEKIAEKAENDGFIIALPDTMTSLYGNQYINSPAVGNYEDFIVSDVLEYLFKRYGKRPVGLFGKSSGGFGAYTLAVRHPDKISGFVDVAGDSGFEYCYLPDFPAAIKYFRRYGVLEWYTYVKSKMNMSPEDMKVVNVLAMSAFYSPDQEAEMGIGLPFDTRTGLLKYDVWDKWRRLDPAFNISNNIDVLKDMAVFMQVGSRDDFHIDIGMEAMHNFLEKNGIVHFFETYDETHFDLDYLYLKSLPLLIELISD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BC2</accession>
    <name>RFCL_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication factor C large subunit</fullName>
            <shortName evidence="1">RFC large subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Clamp loader large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rfcL</name>
        <name type="ordered locus">TV0534</name>
        <name type="ORF">TVG0524887</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family. RfcL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916792.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BC2"/>
    <dbReference type="SMR" id="Q97BC2"/>
    <dbReference type="STRING" id="273116.gene:9381318"/>
    <dbReference type="PaxDb" id="273116-14324749"/>
    <dbReference type="GeneID" id="1441050"/>
    <dbReference type="KEGG" id="tvo:TVG0524887"/>
    <dbReference type="eggNOG" id="arCOG00470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027255_1_1_2"/>
    <dbReference type="OrthoDB" id="8658at2157"/>
    <dbReference type="PhylomeDB" id="Q97BC2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01508">
        <property type="entry name" value="RfcL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023935">
        <property type="entry name" value="Rep_factor-C_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003229">
        <property type="entry name" value="PRK04195.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135969" description="Replication factor C large subunit">
        <location>
            <begin position="1"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="46"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01508"/>
        </source>
    </evidence>
    <sequence length="420" mass="48327" checksum="D2F56F90C7CA31F9" modified="2001-10-01" version="1">MEWADKYRPKRIEDLIVSEEIRQKIQSWIDAWEEGSPKKRALILYGVQGSGKTSAAYAIAGTFGLPVVEMNASEQRNRESMKATALMASLYADLGASDFRKPSKVILIDEADNIFESNNPKRGGDTGGVYELSKIVKETRNPVIITMNDFYEFRKKNYSSEVINNSESIEFKPYARRLDRNYNEFKKNVRNRIKWIINQEGFSLPDDIINSIIDKNAPDIRSIINDVEAAAVSQSSISQNNDRDTVESVYYLVDKAFKKNYDDTLKSIYGSDVDSDYFINWVEENLPSKTDDISDLNSAYEQLSFADHILWAIERKRHFDLMTFPMEIAGGLAYYIENPKHEYVKFHSPSYINSMSRSKERRHALNAVSLKIGLLLHMSGVESLNYVWFYRLMFKVNAGFRDTVYERLNLTQGEEAVLES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-05" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BD5</accession>
    <name>PURL_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="1">FGAR amidotransferase II</shortName>
            <shortName evidence="1">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purL</name>
        <name type="ordered locus">TV0521</name>
        <name type="ORF">TVG0510425</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916779.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BD5"/>
    <dbReference type="SMR" id="Q97BD5"/>
    <dbReference type="STRING" id="273116.gene:9381305"/>
    <dbReference type="PaxDb" id="273116-14324736"/>
    <dbReference type="GeneID" id="1441037"/>
    <dbReference type="KEGG" id="tvo:TVG0510425"/>
    <dbReference type="eggNOG" id="arCOG00641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003100_0_1_2"/>
    <dbReference type="OrthoDB" id="8251at2157"/>
    <dbReference type="PhylomeDB" id="Q97BD5"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002290">
        <property type="entry name" value="PRK01213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100528" description="Phosphoribosylformylglycinamidine synthase subunit PurL">
        <location>
            <begin position="1"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="332"/>
            <end position="334"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="520"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="557"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="558"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="560"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <sequence length="759" mass="84194" checksum="EBDDF8DC566FAE6A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BE6</accession>
    <name>Q97BE6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0499542</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916768.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BE6"/>
    <dbReference type="STRING" id="273116.gene:9381293"/>
    <dbReference type="PaxDb" id="273116-14324725"/>
    <dbReference type="GeneID" id="1441026"/>
    <dbReference type="KEGG" id="tvo:TVG0499542"/>
    <dbReference type="eggNOG" id="arCOG03650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_20_3_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="PhylomeDB" id="Q97BE6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="517" mass="56336" checksum="68E96777DB8B9A2C" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BG3</accession>
    <name>Q97BG3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0477415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916751.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BG3"/>
    <dbReference type="STRING" id="273116.gene:9381274"/>
    <dbReference type="PaxDb" id="273116-14324707"/>
    <dbReference type="GeneID" id="1441009"/>
    <dbReference type="KEGG" id="tvo:TVG0477415"/>
    <dbReference type="eggNOG" id="arCOG03422">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174518_0_0_2"/>
    <dbReference type="OrthoDB" id="55560at2157"/>
    <dbReference type="PhylomeDB" id="Q97BG3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010863">
        <property type="entry name" value="EarA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07381">
        <property type="entry name" value="EarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12937" checksum="170C690A590012B2" modified="2001-10-01" version="1">MEPIIDGNILRSLNRSELRRKVLFYLLSIYPYRSYLSEISRAVKSDPSNVKGCLEGLGVRYTGEESLIGLGLVIVEQSKNGFKYFKINPEIVDEVRNMKVMFNDKKVFTVEIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BH4</accession>
    <name>RS27A_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S27ae</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps27ae</name>
        <name type="ordered locus">TV0481</name>
        <name type="ORF">TVG0468494</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916740.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BH4"/>
    <dbReference type="SMR" id="Q97BH4"/>
    <dbReference type="STRING" id="273116.gene:9381263"/>
    <dbReference type="PaxDb" id="273116-14324696"/>
    <dbReference type="GeneID" id="1440998"/>
    <dbReference type="KEGG" id="tvo:TVG0468494"/>
    <dbReference type="eggNOG" id="arCOG04183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179743_2_0_2"/>
    <dbReference type="OrthoDB" id="25142at2157"/>
    <dbReference type="PhylomeDB" id="Q97BH4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00777">
        <property type="entry name" value="Ribosomal_eS31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022845">
        <property type="entry name" value="Ribosomal_eS31_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001669">
        <property type="entry name" value="PRK00432.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000137710" description="Small ribosomal subunit protein eS31">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="55" mass="6392" checksum="D7CF2CDF894A339D" modified="2001-10-01" version="1">MQKRELYEIADGKLVRKHRFCPRCGPGVFLAEHADRFTCGRCGYTEFKKVKKAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BI3</accession>
    <name>Q97BI3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0461535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916732.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BI3"/>
    <dbReference type="STRING" id="273116.gene:9381254"/>
    <dbReference type="PaxDb" id="273116-14324687"/>
    <dbReference type="GeneID" id="1440990"/>
    <dbReference type="KEGG" id="tvo:TVG0461535"/>
    <dbReference type="eggNOG" id="arCOG07415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830679_0_0_2"/>
    <dbReference type="OrthoDB" id="57476at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="16047" checksum="30306A0C8C47B2CB" modified="2001-10-01" version="1">MDENKEKDINDISKELNKLKPTIDLLKSMQETGMTETLKYLVENYDTIFNLTARMDIYDIATGLKKLSKLAASLSSIDDNYFDELARIVKNLPEVIEKSNKAADENKNIGVLGLLKLMRSNDILFLIFMALEISRELRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BJ0</accession>
    <name>Q97BJ0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0454214 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0454214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BJ0"/>
    <dbReference type="PaxDb" id="273116-14324680"/>
    <dbReference type="KEGG" id="tvo:TVG0454214"/>
    <dbReference type="HOGENOM" id="CLU_1127143_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030987">
        <property type="entry name" value="AbiV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04498">
        <property type="entry name" value="AbiV_defense"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18728">
        <property type="entry name" value="HEPN_AbiV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="246" mass="28951" checksum="DF2A4B8933C9837A" modified="2001-10-01" version="1">MADRREHDDAIRKHIFTEHDCKLCMRNAERLLDDASNNSLSIQTRMALLELSFEETAKSILLYFYLLSHDKGQLLVKGSVEENLRDIFKNHKTKIEIMKNVMSFLAENIPAFDMASDLDYIERIVRYGTEGEKRDSREAKISMARQIALDDRSSIIKKMVKEIIDFLNLQDVDYYNDLKNKSLYVDIDPSTDRLQLPLEPKNKKIMDYMEFFIIVQLADQWDTFGEKKKVGDLINKYRNRLKVLIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-05" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BJ9</accession>
    <name>RADA_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">radA</name>
        <name type="ordered locus">TV0456</name>
        <name type="ORF">TVG0443339</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916715.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BJ9"/>
    <dbReference type="SMR" id="Q97BJ9"/>
    <dbReference type="STRING" id="273116.gene:9381237"/>
    <dbReference type="PaxDb" id="273116-14324671"/>
    <dbReference type="GeneID" id="1440973"/>
    <dbReference type="KEGG" id="tvo:TVG0443339"/>
    <dbReference type="eggNOG" id="arCOG00415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041732_0_0_2"/>
    <dbReference type="OrthoDB" id="31129at2157"/>
    <dbReference type="PhylomeDB" id="Q97BJ9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19515">
        <property type="entry name" value="archRadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002052">
        <property type="entry name" value="DNA repair protein RAD51 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00348">
        <property type="entry name" value="RadA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011938">
        <property type="entry name" value="DNA_recomb/repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02236">
        <property type="entry name" value="recomb_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150110" description="DNA repair and recombination protein RadA">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="115"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00348"/>
        </source>
    </evidence>
    <sequence length="323" mass="35550" checksum="4863E463AB4CD77E" modified="2001-10-01" version="1">MEDNEENKEKKTTLEDLPGVGEATAEKLRENGYDDIMAIAVASPKDLSDVTGIGEGAAAKIIAAARKFADIGNFETGEEILERRKTIQKLTTGSKNLDDLLGGGLETQAITEFFGEFGSGKTQIMHQLAVNCTMPKEKGGFDSDVMMIDTENTFRPERIIQMAKSKGLDPDETLKRIHVARAYNSHHQILLAEKAQETAKEFNIRLLIVDSLTAHFRSEYVGRGSLAERQQLLNKHMHDLLRFGTIYNAVIAVTNQVSARPDVFFGDPMAPIGGNIVGHTATFRVYLRKSKGGKRIARLIDSPYLPEGETVIQISEEGVNDGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BM4</accession>
    <name>Q97BM4_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-amylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0421416</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 57 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916689.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BM4"/>
    <dbReference type="SMR" id="Q97BM4"/>
    <dbReference type="STRING" id="273116.gene:9381210"/>
    <dbReference type="CAZy" id="GH57">
        <property type="family name" value="Glycoside Hydrolase Family 57"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324646"/>
    <dbReference type="DNASU" id="1440947"/>
    <dbReference type="GeneID" id="1440947"/>
    <dbReference type="KEGG" id="tvo:TVG0421416"/>
    <dbReference type="eggNOG" id="arCOG03278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033691_0_0_2"/>
    <dbReference type="OrthoDB" id="64936at2157"/>
    <dbReference type="PhylomeDB" id="Q97BM4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10795">
        <property type="entry name" value="GH57N_MJA1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052046">
        <property type="entry name" value="GH57_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004300">
        <property type="entry name" value="Glyco_hydro_57_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306:SF1">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03065">
        <property type="entry name" value="Glyco_hydro_57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 57 N-terminal" evidence="3">
        <location>
            <begin position="6"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="378" mass="44957" checksum="49FCCF63BEE6B3A7" modified="2001-10-01" version="1">MKDVMFYFEIHQPFRLRPTNLYDVKSEQNVFWDEKNEEIFRRVAERSYIPATRNLMEYGIKSSFSITGTAVEQALMYNTKVIDAIDDYVKSGLCEMLSETYYHSLASIWNYDEFKRQVDMHRDLMKRIFNVVPKVFRNTELIYDDRIAEMVKRMGFTSIITEGTDSIVKDHSPNYRYASPSGLNLYLRNYVMSDNISFRFSNTKWKDYPLTADKYAKWINESEGDMVNLFMDYETFGEHQTQETGIFDFMKYLPVYFRDYGIETITISEAEKRHRVKDVLSIPETISWADTRRDLSAWLENEMQIDTFDRIKKCREKAGRLWGYLQTSDIIYYMSIGLEQDFTVHEYFNPYKSPYLAFIYTNYALDKICGSSYGKNEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-31" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BN6</accession>
    <name>DHYS_THEVO</name>
    <protein>
        <recommendedName>
            <fullName>Probable deoxyhypusine synthase</fullName>
            <shortName>DHS</shortName>
            <ecNumber>2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">dys</name>
        <name type="ordered locus">TV0419</name>
        <name type="ORF">TVG0406345</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916676.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BN6"/>
    <dbReference type="SMR" id="Q97BN6"/>
    <dbReference type="STRING" id="273116.gene:9381197"/>
    <dbReference type="PaxDb" id="273116-14324634"/>
    <dbReference type="GeneID" id="1440934"/>
    <dbReference type="KEGG" id="tvo:TVG0406345"/>
    <dbReference type="eggNOG" id="arCOG04142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039781_1_0_2"/>
    <dbReference type="OrthoDB" id="17730at2157"/>
    <dbReference type="PhylomeDB" id="Q97BN6"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000010">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00153">
        <property type="entry name" value="DHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022899">
        <property type="entry name" value="Deoxyhypus_synthase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002294">
        <property type="entry name" value="PRK01221.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134511" description="Probable deoxyhypusine synthase">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="310" mass="34951" checksum="8B9BF56AC930F920" modified="2001-10-01" version="1">MDRKELLSKPVQDLRIDGNTTLSNLMAQFSNIGGFTAAKLYEAHSIISDMFLEDNTTFLSFPADIISTGLRGLINDVVKRKLVDVIITTSGTLDHDIARTFGKYYCGSFNYSDVELREININRLGNVLVPDESYGELIEEKVMEQLEKLYSIKKEWATVDLIKEIGLSINNESSILYNAAKNDIPIFVPGITDGSFGSQLWSFYEQHHDFKINLLEDEHRLSDIIFDAKKTGAIMVGGGISKHHTIWWNQFRDGLDYAVYITTAQEYDGSLSGAKLEEAISWKKVRPNARFVNIYGDATVIMPILMAPFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-10" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BP2</accession>
    <name>IF2A_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">aIF2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif2a</name>
        <name type="ordered locus">TV0413</name>
        <name type="ORF">TVG0401470</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916670.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BP2"/>
    <dbReference type="SMR" id="Q97BP2"/>
    <dbReference type="STRING" id="273116.gene:9381191"/>
    <dbReference type="PaxDb" id="273116-14324628"/>
    <dbReference type="GeneID" id="1440928"/>
    <dbReference type="KEGG" id="tvo:TVG0401470"/>
    <dbReference type="eggNOG" id="arCOG04107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033458_0_2_2"/>
    <dbReference type="OrthoDB" id="84794at2157"/>
    <dbReference type="PhylomeDB" id="Q97BP2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00231">
        <property type="entry name" value="eIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022964">
        <property type="entry name" value="TIF2_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003062">
        <property type="entry name" value="PRK03987.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003064">
        <property type="entry name" value="PRK03987.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000137405" description="Translation initiation factor 2 subunit alpha">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="10"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00231"/>
        </source>
    </evidence>
    <sequence length="254" mass="28831" checksum="C6EB019E44BB52CF" modified="2001-10-01" version="1">MNIKLLPEVGDLVVVKITEVKNFGANGILEEYPGVEGYIHISEVATGWVKHIRSYLREGQRVVCKVINVNQERKNVDLSLKRVNQHQSREKIAEWKNEQKADKLFEIVCSKLNKDLEACKKEFGVRLVELYGTLFAAFESAAAAEGEWLPDINGDWKDTFVEVAKENITIPEVSVAGYFEAYSLASDGVEKIKEVLTIPPEIGKAELEYVGAPRYRIVVRDKDYKKAEETLKKIVQSVTDKAKKLQVELEFSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BP3</accession>
    <name>Q97BP3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0400558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916668.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BP3"/>
    <dbReference type="STRING" id="273116.gene:9381190"/>
    <dbReference type="PaxDb" id="273116-14324627"/>
    <dbReference type="GeneID" id="1440926"/>
    <dbReference type="KEGG" id="tvo:TVG0400558"/>
    <dbReference type="eggNOG" id="arCOG00348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075000_1_0_2"/>
    <dbReference type="OrthoDB" id="31247at2157"/>
    <dbReference type="PhylomeDB" id="Q97BP3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004426">
        <property type="entry name" value="MJ1210-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00162">
        <property type="entry name" value="proteasome assembly chaperone family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF7">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27792" checksum="C7CEF97F71E99A6B" modified="2001-10-01" version="1">MEKIIVVKYRDVKLKSPILIGGLPGIGNVGKIAADYIVEKLNMVKIYDIFSEYLPPQVFINDDSEIHLVRNSIYYKKMRGKHDLLVLGGDFQGTTQEAQYELSYKMLELAKDMNVSEVYTLGGYSVGKIVERPRVLGAVTDKKLIPELEKNGVIFPKGEPGGGIVGSAGLILGLGMEIFKINGACLMGETSGYFADPKSAMEVLNVLQKQIGIKIDMSDIETRSKQIEQITGKMQEEFQNKQGKEDLGYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BP9</accession>
    <name>Q97BP9_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0394567</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BP9"/>
    <dbReference type="STRING" id="273116.gene:9381184"/>
    <dbReference type="PaxDb" id="273116-14324621"/>
    <dbReference type="KEGG" id="tvo:TVG0394567"/>
    <dbReference type="eggNOG" id="arCOG02881">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083235_0_0_2"/>
    <dbReference type="OrthoDB" id="57479at2157"/>
    <dbReference type="PhylomeDB" id="Q97BP9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="2"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="146"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="271" mass="29467" checksum="5CC4E384339F9131" modified="2001-10-01" version="1">MAVLIVSSDRFFDEATAAMGRIGFSDRFSGSIFAAMVAVADEISLSIFSVILRTPLVGFGAVEGSNLVTLLFFVVWMPLFSIQKTRAFRADAVFVLLLSIITYYFSTVSSDFKIIAGILLSLFIVPYLMVYVRMPRGKSSTTEEFSLIMLVLSIVLLTAASQIIVEDTIALSELDHIDIAALSIYGIGIASAIPETVMIIVASIKKKVDISIGVLAGSSVYKMSIIPGLLIMTGKIETTGLDLPLMTIMILSVIFLFYTLYGTIKFKGGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BS0</accession>
    <name>Q97BS0_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0372401 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0372401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916639.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BS0"/>
    <dbReference type="STRING" id="273116.gene:9381162"/>
    <dbReference type="PaxDb" id="273116-14324600"/>
    <dbReference type="GeneID" id="1440897"/>
    <dbReference type="KEGG" id="tvo:TVG0372401"/>
    <dbReference type="eggNOG" id="arCOG07400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691633_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="155" mass="17475" checksum="F01CCBEA8F8EC8B3" modified="2001-10-01" version="1">MTLVITDVLRYYSGDTNDEKISKLYITASRKVLGQAYDLKNGTVLQGRLMDLSVAPGKEKITETAVPGRDRLIGTKLEAYYFSAGQSNPTADRDYIFFSSKFSHILTEYAIFAEEYEVKVEFVSAAYKLKKVKLYTKGTVMDSYSEEKSEGDVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BS6</accession>
    <name>Q97BS6_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0368053 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0368053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_241760304.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BS6"/>
    <dbReference type="STRING" id="273116.gene:9381156"/>
    <dbReference type="PaxDb" id="273116-14324594"/>
    <dbReference type="GeneID" id="1440891"/>
    <dbReference type="KEGG" id="tvo:TVG0368053"/>
    <dbReference type="eggNOG" id="arCOG05384">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140103_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="112" mass="12892" checksum="2DB393BF25E74970" modified="2001-10-01" version="1">MDRYSLKLYYASMIVYIFGSITLILYTLIIKPIALMYHEPINQMVSPVFGNYARYLFSLELFTMIIMVVSLILFLLSIYHNHIRNGKISKPTIITPVLLFAFAFVLLGVSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BS8</accession>
    <name>Q97BS8_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytochrome b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">TVG0367524</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="eggNOG" id="arCOG01721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059314_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:electron transfer activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:oxidoreductase activity"/>
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        <property type="term" value="P:respiratory electron transport chain"/>
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        <property type="entry name" value="Cytochrome Bc1 Complex, Chain C"/>
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        <property type="entry name" value="Cyt_b/b6_C"/>
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        <property type="entry name" value="Cytb/b6-like_sf"/>
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        <property type="entry name" value="Di-haem_cyt_TM"/>
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        <property type="entry name" value="CYTOCHROME B"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81648">
        <property type="entry name" value="a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transmembrane di-heme cytochromes"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CYTB_NTER"/>
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    <keyword evidence="3" id="KW-0349">Heme</keyword>
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    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="181"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="214"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="234"/>
            <end position="256"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <end position="318"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="330"/>
            <end position="349"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 N-terminal region profile" evidence="11">
        <location>
            <begin position="9"/>
            <end position="224"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAB59519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="391" mass="44333" checksum="375BA60C2158D8E9" modified="2001-10-01" version="1">MTEKNEYDLTKLTKPDEEFEGSYKIVLRPISKKEFRLDYWTGSFLMAAVAYLAVSGMLLFYYYQSGHPFSSTISIISSVPFGYALLTSHLYMAYAMIVLVYAHMLRNYFVGAYKGKWRWLQWILGVILFILVYTTAIVGYMLTYTYISVAATHVGELLIERSIIGRLLPGLANWLVSILIGDGTTSQTFSHILGLHVMILSTLIIVIAFVHFFLFEKSGPYGVKYDKNEKMVPWFPVNLLYTIFLSLMFIGVILIFSAAFPQVIPAAYGLPVYGTLPFPDWYILPVYKLMDTAGYGLTTGGVPLVIVFFLFLLILPFIDRYSGSHPLDRPMITAFGIFIIIGIPVIALWGASQPGLTQTRLLTMFMWWGLTFVSIATVYAMRFARKDGEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BV1</accession>
    <name>Q97BV1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF106 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">TVG0346559</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916608.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="tvo:TVG0346559"/>
    <dbReference type="eggNOG" id="arCOG02673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1227721_0_0_2"/>
    <dbReference type="OrthoDB" id="84619at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038978">
        <property type="entry name" value="MJ0935"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="21"/>
            <end position="39"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
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        <location>
            <begin position="141"/>
            <end position="167"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAB59496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="26685" checksum="038C1137567D818D" modified="2001-10-01" version="1">MTETYNKEQQEAMKKMMSFQMLYMLIMLGSLFIVITPSSRDAVGNLLNVVLIPTIGFGYRYPLLSIILTGVIIGVILSIPRYFFTDWVKMGKMQNTMKAFNEAIRAAYRERDMKKINKLNSMRMQMSMDQYQLSMNTMKPLLVISVLTILFYAWLFVFVGKIPYNYIAFPWDFNINISTAHFWIMPYWIFMYFLTEIVVSYFITMIMKYIDFTLRLRRISRETSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BW5</accession>
    <name>RL24_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein uL24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl24</name>
        <name type="ordered locus">TV0340</name>
        <name type="ORF">TVG0338390</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Located at the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916594.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BW5"/>
    <dbReference type="SMR" id="Q97BW5"/>
    <dbReference type="STRING" id="273116.gene:9381117"/>
    <dbReference type="PaxDb" id="273116-14324555"/>
    <dbReference type="GeneID" id="1440852"/>
    <dbReference type="KEGG" id="tvo:TVG0338390"/>
    <dbReference type="eggNOG" id="arCOG04094">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093240_1_0_2"/>
    <dbReference type="OrthoDB" id="10899at2157"/>
    <dbReference type="PhylomeDB" id="Q97BW5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130785" description="Large ribosomal subunit protein uL24">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="145" mass="16315" checksum="173E9B9E5AC9BCD8" modified="2001-10-01" version="1">MYDKLNVALSKDLRKKYGIRSFPIVKGDVVKVVSGARKGEGGKVAEVDHHSGLVIVEGITIAKIDGKQKGFGISPEKLQITHLDLSRSERFQKIKELANIKHITIQEEPIQEEQQKTEETKQEIAPEEVEAKEAQDKQEVKENDQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-18" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BW7</accession>
    <name>RS17_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps17</name>
        <name type="ordered locus">TV0338</name>
        <name type="ORF">TVG0337549</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916592.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BW7"/>
    <dbReference type="SMR" id="Q97BW7"/>
    <dbReference type="STRING" id="273116.gene:9381115"/>
    <dbReference type="PaxDb" id="273116-14324553"/>
    <dbReference type="GeneID" id="1440850"/>
    <dbReference type="KEGG" id="tvo:TVG0337549"/>
    <dbReference type="eggNOG" id="arCOG04096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_0_3_2"/>
    <dbReference type="OrthoDB" id="10698at2157"/>
    <dbReference type="PhylomeDB" id="Q97BW7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_A">
        <property type="entry name" value="Ribosomal_uS17_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019978">
        <property type="entry name" value="Ribosomal_uS17_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000232620" description="Small ribosomal subunit protein uS17">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="109" mass="12410" checksum="D07F80B8424FF314" modified="2001-10-01" version="1">MQARNIGLDVSVPEKECTDPHCPFHGNLPVRGQVLTGKVISTAMTRSVVVAREYQQYIPKYERKATKIKKYHVHVPDCIELRVGDTVRFAECRKLAKTISFVVVEKVKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BX5</accession>
    <name>RL23_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl23</name>
        <name type="ordered locus">TV0330</name>
        <name type="ORF">TVG0334241</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L29.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916584.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BX5"/>
    <dbReference type="SMR" id="Q97BX5"/>
    <dbReference type="STRING" id="273116.gene:9381107"/>
    <dbReference type="PaxDb" id="273116-14324545"/>
    <dbReference type="GeneID" id="1440842"/>
    <dbReference type="KEGG" id="tvo:TVG0334241"/>
    <dbReference type="eggNOG" id="arCOG04072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037562_4_2_2"/>
    <dbReference type="OrthoDB" id="7751at2157"/>
    <dbReference type="PhylomeDB" id="Q97BX5"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_uL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_uL23_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000272959" description="Large ribosomal subunit protein uL23">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="83" mass="9259" checksum="780FD543F16B6DE0" modified="2001-10-01" version="1">MGDVILSPVSTEKTALLAEKENKLTLIVDRKATKEDIKREVESRFSVKVEGINILITKKGKKAIVKLAKEYSAEEIAERIGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C20</accession>
    <name>Q97C20_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0296525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916540.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C20"/>
    <dbReference type="PaxDb" id="273116-14324499"/>
    <dbReference type="GeneID" id="1440798"/>
    <dbReference type="KEGG" id="tvo:TVG0296525"/>
    <dbReference type="eggNOG" id="arCOG07360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1036729_0_0_2"/>
    <dbReference type="OrthoDB" id="378281at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="260" mass="29692" checksum="B2C04BE439EE977F" modified="2001-10-01" version="1">MQIEVSLPKEHGLIVVWFNTLIISVFVEKSFPLYGALTLLIMATTFFIYDPILVVLRLFKAGRNPLGTLLRKYPYVILFSGIILIWVLYGYARGMLPGISVAVFIFSLLLFLGFFWLGERKIYTRAISILTLTSFFLIISGAFTTRLTLKSIDIFVILSAVEIFLAAIPVEIVNSRIHKFRFNALLFRSLFPIYVIVLLALYILLIKNLTEFLFFFAITTAAFLSVPILRSMTLKRIGLVATLFNIILLAFLLLFYFKSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C26</accession>
    <name>Y279_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0200 protein TV0279</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0279</name>
        <name type="ORF">TVG0291894</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0200 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916534.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C26"/>
    <dbReference type="SMR" id="Q97C26"/>
    <dbReference type="STRING" id="273116.gene:9381053"/>
    <dbReference type="PaxDb" id="273116-14324493"/>
    <dbReference type="GeneID" id="1440792"/>
    <dbReference type="KEGG" id="tvo:TVG0291894"/>
    <dbReference type="eggNOG" id="arCOG01045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096329_1_0_2"/>
    <dbReference type="OrthoDB" id="85381at2157"/>
    <dbReference type="PhylomeDB" id="Q97C26"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01111">
        <property type="entry name" value="UPF0200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022970">
        <property type="entry name" value="NTP_hydrolase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930:SF1">
        <property type="entry name" value="DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930">
        <property type="entry name" value="UPF0200 PROTEIN MJ1399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000094537" description="UPF0200 protein TV0279">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="6"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01111"/>
        </source>
    </evidence>
    <sequence length="179" mass="20737" checksum="0D0B71F41C83138A" modified="2001-10-01" version="1">MIIVTGMPGAGKDEFVKVARSLGFMDLHMGNTVREYASRNGIPDDDKEIGNFAASERKKFGMDIWARRTAEKIESDELTVIDGVRNKEEMDYFSKFSKSIYVVAIFANRKTRLERILKRDRPDDIRSMEGLIERDNRELSWGIGNVIALADYMIVNDESLEIFYERSRKLLFDHFLIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C39</accession>
    <name>Q97C39_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 15">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="8 15">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">TVG0278338</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="8 15"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916522.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C39"/>
    <dbReference type="STRING" id="273116.gene:9381039"/>
    <dbReference type="PaxDb" id="273116-14324480"/>
    <dbReference type="GeneID" id="1440780"/>
    <dbReference type="KEGG" id="tvo:TVG0278338"/>
    <dbReference type="eggNOG" id="arCOG05046">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054856_2_1_2"/>
    <dbReference type="OrthoDB" id="53073at2157"/>
    <dbReference type="PhylomeDB" id="Q97C39"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000191">
        <property type="entry name" value="ribulose-phosphate 3-epimerase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="NCBIfam" id="TIGR01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAB59408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="211" mass="23586" checksum="394F722C6751E815" modified="2001-10-01" version="1">MLVSPSIVAASLTKFQAQVKECEDAGAYSFHLDVMDGNFVPNITVGPDFYFALRSISKIPIDTHLMVDRPDYYYEKFLSQNDRVLIHYESPINIKGLIKKMENEGVKYGLVINPNTPFSKVSDLLERAEILLIMSVYPGFSGQKFIDVSEKIKEARNYIKEMGLRTMIEVDGGINENTAKIVKNAGADIVVSASYLFSGDITDRIKTLSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C49</accession>
    <name>Q97C49_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0268261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916513.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C49"/>
    <dbReference type="PaxDb" id="273116-14324470"/>
    <dbReference type="GeneID" id="1440771"/>
    <dbReference type="KEGG" id="tvo:TVG0268261"/>
    <dbReference type="eggNOG" id="arCOG05344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800770_0_0_2"/>
    <dbReference type="OrthoDB" id="37025at2157"/>
    <dbReference type="PhylomeDB" id="Q97C49"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="40819" checksum="4840EA6749C6A0F4" modified="2001-10-01" version="1">MANNYPTVRKYGYTSELMAIIGYTIPTFILVMPAFYTSYISNEFQIPYYTVFIIIGSPFIGRFLGAYLYSKFRFLGTPFSYSMFAIGILSIAMAFEKSVTMMAMDRFLIGISFGIVTSFAVEVAVRTRNQIVIGLTTGGWALGWILSAIVSMYMPFKYALLVSGISIPLSLLGLLIRNVPIGHVISTGFNFSPYSFLIYFLAFEPAFVLEIAPKLLGSTAFVESLIAYLVAIPAYMVFPILGKRYGFKNMIYVTSVVTIISSIFFFIFLSLEAAVVFTVMGLTVNAIIPGFLRSSNIDPEKIGPSLNFSAINGFITPTLVYVIGYPRYSSLSLTLLSMTLLLALAFSRISLGSHMHSFGHKRSLRNHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C56</accession>
    <name>Q97C56_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histidine triad protein [HIT]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0263349</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C56"/>
    <dbReference type="STRING" id="273116.gene:9381021"/>
    <dbReference type="PaxDb" id="273116-14324463"/>
    <dbReference type="KEGG" id="tvo:TVG0263349"/>
    <dbReference type="eggNOG" id="arCOG00419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_3_2_2"/>
    <dbReference type="OrthoDB" id="26806at2157"/>
    <dbReference type="PhylomeDB" id="Q97C56"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648:SF1">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="HIT FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="21"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="111"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="158" mass="18265" checksum="51F55EC2B9ECF8AC" modified="2001-10-01" version="1">MYVPGKQLTLFDEENEVVKCKFCDIISGKIASNVVYSDEHFIAFLDHKPLFKGHTLLVPRKHFENIYEMDEETSSKLMHEVQLISRAVEIATSSDGTFIAINNKVSQSVPHVHVHIVPRKQKDGLKGFFWPRQEYGEGEDREYANKIREQIEKISSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C86</accession>
    <name>Q97C86_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cyclomaltodextrinase [amylase]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0226058</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C86"/>
    <dbReference type="SMR" id="Q97C86"/>
    <dbReference type="STRING" id="273116.gene:9380987"/>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="PaxDb" id="273116-14324431"/>
    <dbReference type="KEGG" id="tvo:TVG0226058"/>
    <dbReference type="eggNOG" id="arCOG02948">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006462_6_4_2"/>
    <dbReference type="PhylomeDB" id="Q97C86"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11338">
        <property type="entry name" value="AmyAc_CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF210">
        <property type="entry name" value="MALTODEXTRIN GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="3">
        <location>
            <begin position="194"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="619" mass="71362" checksum="8C15009552087DE8" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C93</accession>
    <name>Q97C93_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH hxlR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0218796</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C93"/>
    <dbReference type="STRING" id="273116.gene:9380980"/>
    <dbReference type="PaxDb" id="273116-14324424"/>
    <dbReference type="KEGG" id="tvo:TVG0218796"/>
    <dbReference type="eggNOG" id="arCOG01057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_5_3_2"/>
    <dbReference type="PhylomeDB" id="Q97C93"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204:SF18">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="4">
        <location>
            <begin position="10"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="122" mass="14011" checksum="94675B53DCC36840" modified="2001-10-01" version="1">MVGSMDESTCPIIEAIREVGKENDLLVIRLLSDNDLGFNQILKLAGKMSPKTLSVTLKRLTEKKIIYRDVVSTQPFRVKYRLTEKGQELRNVLNDLGQWGERWILNNESQSAVNTENYRLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CA1</accession>
    <name>Q97CA1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thioredoxin/glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0208121</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916458.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CA1"/>
    <dbReference type="STRING" id="273116.gene:9380972"/>
    <dbReference type="PaxDb" id="273116-14324416"/>
    <dbReference type="GeneID" id="1440718"/>
    <dbReference type="KEGG" id="tvo:TVG0208121"/>
    <dbReference type="eggNOG" id="arCOG01218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082677_0_0_2"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="PhylomeDB" id="Q97CA1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02975">
        <property type="entry name" value="PfPDO_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02973">
        <property type="entry name" value="TRX_GRX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011903">
        <property type="entry name" value="TON_0319-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02187">
        <property type="entry name" value="PDO_seleno_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170:SF1">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170">
        <property type="entry name" value="GLUTAREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="3">
        <location>
            <begin position="35"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin-like fold" evidence="3">
        <location>
            <begin position="138"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="220" mass="24716" checksum="4DB64BAED6995BBE" modified="2001-10-01" version="1">MATLIRKEDREYLKGEFDKYLKNDVDLVVFTSNDENCRYCKETVQLATEVSEINPKIHLKVYNFDEDKDQVKAYGVEKYPATIVSKAGVEDGRIVYYGLPSGYEFGSLIEDLKNVSVGDADVSSKAAELISKIDKPITIKVYVTPTCPYCPRAVGTAHKFALMNPNIKGEMIEALEFENEAEEVGVSSVPHIVINNDVTFIGAYPDDQFAEYVMEAYDHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CE2</accession>
    <name>Q97CE2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper resistance protein D domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0170162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CE2"/>
    <dbReference type="PaxDb" id="273116-14324374"/>
    <dbReference type="KEGG" id="tvo:TVG0170162"/>
    <dbReference type="eggNOG" id="arCOG05392">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590962_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23351" checksum="D724C455402F700F" modified="2001-10-01" version="1">MIICYKSDIDIYLSYCLAIKTKFVAESLLIYTHPRYNDMSSLDLIMAVLIIHVFASIMFIGGSFFIWFIVWPASYKMTKDESMRTKIVGIIGRYFGWWTDATIAILVVSGGYLGYEYIHGNFGLLTTTIGGKILLAKVIVVWIMIVLMYANNIYHGKLIMRLAEEKKYDEMKRIRKITHVASFITLGLILAIMGLAVLLQFFMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CF3</accession>
    <name>Q97CF3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3311 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0153694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916403.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CF3"/>
    <dbReference type="PaxDb" id="273116-14324362"/>
    <dbReference type="GeneID" id="1441633"/>
    <dbReference type="KEGG" id="tvo:TVG0153694"/>
    <dbReference type="HOGENOM" id="CLU_183045_1_1_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021741">
        <property type="entry name" value="DUF3311"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11755">
        <property type="entry name" value="DUF3311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7789" checksum="F8E65F32A9C00F8A" modified="2001-10-01" version="1">MNSKYKKFLLLLIPYIAILVIAPLLNSIHILVLDMPLIMFWLFIWFFLTPIVTYMIYREDRRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CG1</accession>
    <name>Q97CG1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0147168 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0147168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CG1"/>
    <dbReference type="STRING" id="273116.gene:9380910"/>
    <dbReference type="PaxDb" id="273116-14324354"/>
    <dbReference type="DNASU" id="1441625"/>
    <dbReference type="KEGG" id="tvo:TVG0147168"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754811_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CG1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13737">
        <property type="entry name" value="DDE_Tnp_1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase DDE" evidence="1">
        <location>
            <begin position="26"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="148" mass="17602" checksum="88A2DD1384B1B467" modified="2001-10-01" version="1">MSRWGKHFKDGRNWPGYNEELVIRSTFLFNLDFVQQWDQEVTNMNRGKRGSPYLFPESFMKFMMMWKQFLDYRALEGMARSLADMKKIPQYGYYTTVWHRIHDMKPTLDISDLRYADLGTDGTGLKTNNARSYRIMKYGDPDSGRRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CG3</accession>
    <name>Q97CG3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TVG0145569 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0145569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CG3"/>
    <dbReference type="STRING" id="273116.gene:9380908"/>
    <dbReference type="PaxDb" id="273116-14324352"/>
    <dbReference type="KEGG" id="tvo:TVG0145569"/>
    <dbReference type="eggNOG" id="arCOG01241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1478996_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CG3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="102"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="182" mass="20658" checksum="D5A96FB2B89A2FC7" modified="2001-10-01" version="1">MARILRKATSRGRVAIAIMAFSGLRPESLGDYEGTDCLRIGDLKEVKLSDEIQFDKIPAKVMVKSKLSKSRQQYFGFIGEEGATYIKEYLEERGLMNRKGFPESYDLKALIKFPYELKSGKENIDIPQYSHIIYDIVPGKFKTVENPDVLILEGLNVLQTRRTDTIPPASELLVSDFLDFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CI1</accession>
    <name>Q97CI1_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc-containing ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">TVG0127036</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 11">Binds 1 zinc ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="10 11">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10 11">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CI1"/>
    <dbReference type="STRING" id="273116.gene:9380888"/>
    <dbReference type="PaxDb" id="273116-14324334"/>
    <dbReference type="KEGG" id="tvo:TVG0127036"/>
    <dbReference type="eggNOG" id="arCOG04548">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507315_0_0_2"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="PhylomeDB" id="Q97CI1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009157">
        <property type="entry name" value="Fd_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053687">
        <property type="entry name" value="Zinc-ferredoxin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041165">
        <property type="entry name" value="ZFX_Thplmales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000068">
        <property type="entry name" value="Zn_Fdx_Sulfol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10 11" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="2 10 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <keyword evidence="5 10 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="117"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000068-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAB59262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="145" mass="16423" checksum="B80BCA8ABCE7219C" modified="2001-10-01" version="1">MYLVKLEELDFKPKPIDEHFLENDKDYPITGQHNNHDVRAEGMQRMDADGKPYPTKLGIHGTHVAVDWDCCIADGACMDVCPVNLYEWNLNPGKSGTGNDKKVEKGSDEWNKYRTDKCDPVRESDCIFCMACESVCPVRAIKITP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-10-25" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CI3</accession>
    <name>NAC_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nascent polypeptide-associated complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nac</name>
        <name type="ordered locus">TV0118</name>
        <name type="ORF">TVG0126024</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Contacts the emerging nascent chain on the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Interacts with the ribosome. Binds ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAC-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916374.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CI3"/>
    <dbReference type="SMR" id="Q97CI3"/>
    <dbReference type="STRING" id="273116.gene:9380886"/>
    <dbReference type="PaxDb" id="273116-14324332"/>
    <dbReference type="GeneID" id="1441604"/>
    <dbReference type="KEGG" id="tvo:TVG0126024"/>
    <dbReference type="eggNOG" id="arCOG04061">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146475_1_0_2"/>
    <dbReference type="OrthoDB" id="53273at2157"/>
    <dbReference type="PhylomeDB" id="Q97CI3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14359">
        <property type="entry name" value="UBA_AeNAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00814">
        <property type="entry name" value="NAC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005231">
        <property type="entry name" value="NAC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00264">
        <property type="entry name" value="archaeal-type nascent polypeptide-associated complex protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000135614" description="Nascent polypeptide-associated complex protein">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="NAC-A/B" evidence="1">
        <location>
            <begin position="5"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00814"/>
        </source>
    </evidence>
    <sequence length="119" mass="13390" checksum="4C282F48C78CDC66" modified="2001-10-01" version="1">MIPGRMNSREMRRLMAQMGIKSTEMDDVKKVIFVGKEKDYVIENAQVTMIEAQGVKTFQVVGDFRETPKKQEGKKEETFPEDDIKLVMDQTGVAREKAIEALKAASGEPAQAILNLTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CK1</accession>
    <name>Q97CK1_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 /pyruvate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0105141</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916357.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CK1"/>
    <dbReference type="STRING" id="273116.gene:9380868"/>
    <dbReference type="PaxDb" id="273116-14324314"/>
    <dbReference type="GeneID" id="1441587"/>
    <dbReference type="KEGG" id="tvo:TVG0105141"/>
    <dbReference type="eggNOG" id="arCOG01052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012907_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q97CK1"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43257">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43257:SF2">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="4">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="319" mass="34936" checksum="CEE3B40370356C87" modified="2001-10-01" version="1">MNIVQALNNAMDIKMAEDNDVVVLGEDVGKDGGVFRVTDGLLAKYGPERVIDTPLTELGIVGMAIGMAVNGLKPIPEIQFQDFIYTAMDQIINQMAKIRYRSGGDYTVPLVLRTPVGGGIKGGLYHSQSGETYFAHTAGLTVVSPSNPYDAKGLLISSIESPDPVIFLEPKRLYRSQKADVPEDKYTVPLRKANLLREGNSVTLVTYGSMVPTVLSTVDKNGIDADVVDLRTIAPLDKDTIISSVKKTGRVVIVHEAPRTLGVGAEVSAMISERAIEYLYAPILRITGPDTPFPYRLEDYYLPNEQRIMAAIKKVMEYR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CR9</accession>
    <name>SYV_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Valine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Valyl-tRNA synthetase</fullName>
            <shortName evidence="1">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">valS</name>
        <name type="ordered locus">TV0032</name>
        <name type="ORF">TVG0034869</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916289.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CR9"/>
    <dbReference type="SMR" id="Q97CR9"/>
    <dbReference type="STRING" id="273116.gene:9380797"/>
    <dbReference type="PaxDb" id="273116-14324246"/>
    <dbReference type="GeneID" id="1441518"/>
    <dbReference type="KEGG" id="tvo:TVG0034869"/>
    <dbReference type="eggNOG" id="arCOG00808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_2"/>
    <dbReference type="OrthoDB" id="23906at2157"/>
    <dbReference type="PhylomeDB" id="Q97CR9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000192">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02005">
        <property type="entry name" value="Val_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022874">
        <property type="entry name" value="Valine-tRNA_ligase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009687">
        <property type="entry name" value="PRK13208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF93">
        <property type="entry name" value="VALINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224638" description="Valine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="521"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02005"/>
        </source>
    </evidence>
    <sequence length="790" mass="91205" checksum="C4FFCA069E8753CF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CS1</accession>
    <name>COBD_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobD</name>
        <name type="ordered locus">TV0030</name>
        <name type="ORF">TVG0029865</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59172" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59172.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CS1"/>
    <dbReference type="SMR" id="Q97CS1"/>
    <dbReference type="STRING" id="273116.gene:9380795"/>
    <dbReference type="PaxDb" id="273116-14324244"/>
    <dbReference type="KEGG" id="tvo:TVG0029865"/>
    <dbReference type="eggNOG" id="arCOG04274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054212_0_2_2"/>
    <dbReference type="OrthoDB" id="46105at2157"/>
    <dbReference type="PhylomeDB" id="Q97CS1"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00380">
        <property type="entry name" value="cobal_cbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002281">
        <property type="entry name" value="PRK01209.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308:SF1">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000150948" description="Probable cobalamin biosynthesis protein CobD">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="304" mass="34484" checksum="947DFE831F2C0756" modified="2003-07-11" version="2">MIVVLIGALSIDIIFGEPKEYIHPVVFSGRVASAIEGYFRKFDNRFRAGILFSIAVIVLTAIPYFLAVYLSSFILVVYVVVSMVILKTTFSITSMGEHIKLITDSLKKGNIMEARMHLSMIVRRDTSRLNENEISSAAIESIAEGLVDGYITPLFFFVFFGLPGAFIARIINTLDSMYGYKDRKNFEFGRFSAFMDTVINYIPARISWFFITFSSDILNYRSKAIPVRRYIRRFDSVNAGWPIASMASALNLRLEKKGHYIVNDDGYQPGVADIEKSMKIYYLAAYSYIVIFVLPLLVIMAVFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-25" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q977Z3</accession>
    <name>ATGT_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-guanine(15) transglycosylase</fullName>
            <ecNumber evidence="1">2.4.2.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-deazaguanine tRNA-ribosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Archaeal tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tgtA</name>
        <name type="ordered locus">TV1524</name>
        <name type="ORF">TVG1580555</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(15) in tRNA + 7-cyano-7-carbaguanine = 7-cyano-7-carbaguanosine(15) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:43164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:10372"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="EC" id="2.4.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeosine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.48" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917753.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q977Z3"/>
    <dbReference type="SMR" id="Q977Z3"/>
    <dbReference type="STRING" id="273116.gene:9382339"/>
    <dbReference type="PaxDb" id="273116-14325763"/>
    <dbReference type="GeneID" id="1442213"/>
    <dbReference type="KEGG" id="tvo:TVG1580555"/>
    <dbReference type="eggNOG" id="arCOG00989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030083_0_0_2"/>
    <dbReference type="OrthoDB" id="6871at2157"/>
    <dbReference type="PhylomeDB" id="Q977Z3"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21149">
        <property type="entry name" value="PUA_archaeosine_TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="entry name" value="ArcTGT, C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01634">
        <property type="entry name" value="TgtA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050076">
        <property type="entry name" value="ArchSynthase1/Queuine_TRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029402">
        <property type="entry name" value="TGT_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038250">
        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004804">
        <property type="entry name" value="TgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00432">
        <property type="entry name" value="arcsn_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14810">
        <property type="entry name" value="TGT_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000247888" description="tRNA-guanine(15) transglycosylase">
        <location>
            <begin position="1"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="553"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01634"/>
        </source>
    </evidence>
    <sequence length="632" mass="71957" checksum="FFB7F1B1543E0C36" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q978M2</accession>
    <name>Q978M2_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG1440155</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917626.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978M2"/>
    <dbReference type="STRING" id="273116.gene:9382201"/>
    <dbReference type="PaxDb" id="273116-14325632"/>
    <dbReference type="DNASU" id="1442084"/>
    <dbReference type="GeneID" id="1442084"/>
    <dbReference type="KEGG" id="tvo:TVG1440155"/>
    <dbReference type="eggNOG" id="arCOG01467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_6_0_2"/>
    <dbReference type="OrthoDB" id="147058at2157"/>
    <dbReference type="PhylomeDB" id="Q978M2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF2">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="20"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="254" mass="28455" checksum="D70712F155D66533" modified="2001-10-01" version="1">MIPPSIRLTLRNLIVNTDPGTLLFLLGLPSFYLIVLGLMFQSIIPNVPFAGKDISYAQFLSPGVVAMQPFIAGSIGGSMLWSDRRWGMFEQLIVGPFHRIDYLLGIIYVSIIFSVGGAFLMFLVSYAITGFLVSYDINLLMIAVVLIVSSILFTSIFLVLSVFIRTIQTYNTVTMFVFFILDFASSAFYPINSRTPLGLRIVSYMNPLTYIVDAVRNMMFYKIGMQDYISLLIVVVLSGFFFLIALVSYGRARV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-19" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q978W7</accession>
    <name>RS6E_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S6e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps6e</name>
        <name type="ordered locus">TV1298</name>
        <name type="ORF">TVG1338731</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917533.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978W7"/>
    <dbReference type="SMR" id="Q978W7"/>
    <dbReference type="STRING" id="273116.gene:9382105"/>
    <dbReference type="PaxDb" id="273116-14325537"/>
    <dbReference type="GeneID" id="1441415"/>
    <dbReference type="KEGG" id="tvo:TVG1338731"/>
    <dbReference type="eggNOG" id="arCOG01946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109671_1_1_2"/>
    <dbReference type="OrthoDB" id="7793at2157"/>
    <dbReference type="PhylomeDB" id="Q978W7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_eS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_eS6_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003294">
        <property type="entry name" value="PRK04290.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000137364" description="Small ribosomal subunit protein eS6">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="128" mass="14112" checksum="D2D29A929B6E2ED9" modified="2001-10-01" version="1">MANSLAIIADPKTGKTYKREIPSERMSSLIGRKIGEEVDGVFFDLVGYKMKITGGSSVDGFAMRPDLQTQGKKQILVKYTSGYRGKNGIRKRITARGSIIGSDITQINLKITQYGPTPIEEKKDDQQA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q978W8</accession>
    <name>IF2G_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="1">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">aIF2-gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-2-gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif2g</name>
        <name type="ordered locus">TV1297</name>
        <name type="ORF">TVG1337512</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917532.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q978W8"/>
    <dbReference type="SMR" id="Q978W8"/>
    <dbReference type="STRING" id="273116.gene:9382104"/>
    <dbReference type="PaxDb" id="273116-14325536"/>
    <dbReference type="GeneID" id="1441414"/>
    <dbReference type="KEGG" id="tvo:TVG1337512"/>
    <dbReference type="eggNOG" id="arCOG01563">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027154_0_1_2"/>
    <dbReference type="OrthoDB" id="7798at2157"/>
    <dbReference type="PhylomeDB" id="Q978W8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000075">
        <property type="entry name" value="Translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00119">
        <property type="entry name" value="eIF_2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022424">
        <property type="entry name" value="TIF2_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03680">
        <property type="entry name" value="eif2g_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137466" description="Translation initiation factor 2 subunit gamma">
        <location>
            <begin position="1"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G" evidence="1">
        <location>
            <begin position="9"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="18"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="46"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="144"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="179"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="21"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="144"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="179"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00119"/>
        </source>
    </evidence>
    <sequence length="411" mass="44715" checksum="FB62895DA9EC0625" modified="2001-10-01" version="1">MISKLKMPQPTVNIGMVGHVDHGKSTLTLALTGVKTDTHSEEIKRGISIKLGYADTPVYKCYDASGNPYYTRQPSENCELERVISIVDAPGHETLMATMLSGSALMNGALLVIAANEHCPQPQTREHLTALEIMGIKNIVIVQNKIDLVTRERALESYKEIKAFVKGSIAENAPIIPVSAYHNTNIDILFEAIEKYIPTPEYNEGSDPIMYIARSFDVNKPGTPIDQIKGGIIGGSLTQGSLKIGDEIEIVPGIQNTRGNKTVWTNVTTEVVSLMAGKYSYDMIKPGGLAAVGTKLDPFLTKGDAFTGRIAGYIGKVPPISFSMRLEAHLLKRVVGSDQELNVEPIRAKETLMFTVATANTVGVVSNVKGTDIEVSLKYPVAAFNGMRVAIGRRVLNRWRLIGYGVIESLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-30" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Q0</accession>
    <name>SYE_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="1">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gltX</name>
        <name type="ordered locus">TV1110</name>
        <name type="ORF">TVG1143889</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917344.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Q0"/>
    <dbReference type="SMR" id="Q979Q0"/>
    <dbReference type="STRING" id="273116.gene:9381909"/>
    <dbReference type="PaxDb" id="273116-14325348"/>
    <dbReference type="GeneID" id="1441226"/>
    <dbReference type="KEGG" id="tvo:TVG1143889"/>
    <dbReference type="eggNOG" id="arCOG04302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_1_3_2"/>
    <dbReference type="OrthoDB" id="10470at2157"/>
    <dbReference type="PhylomeDB" id="Q979Q0"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003169">
        <property type="entry name" value="PRK04156.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000119734" description="Glutamate--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02076"/>
        </source>
    </evidence>
    <sequence length="548" mass="63185" checksum="09E473C1E7B3F926" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q979T6</accession>
    <name>Q979T6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1100740</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917303.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979T6"/>
    <dbReference type="STRING" id="273116.gene:9381870"/>
    <dbReference type="PaxDb" id="273116-14325312"/>
    <dbReference type="GeneID" id="1441185"/>
    <dbReference type="KEGG" id="tvo:TVG1100740"/>
    <dbReference type="eggNOG" id="arCOG00329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_6_2_2"/>
    <dbReference type="PhylomeDB" id="Q979T6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05531">
        <property type="entry name" value="POLBc_B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004416">
        <property type="entry name" value="PRK05761.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="8">
        <location>
            <begin position="246"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="619" mass="71286" checksum="48F9229DCA5016C4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q979Z0</accession>
    <name>Q979Z0_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lipoate--protein ligase</fullName>
            <ecNumber evidence="3">6.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">TVG1043834</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-lysyl-[lipoyl-carrier protein] + (R)-lipoate + ATP = N(6)-[(R)-lipoyl]-L-lysyl-[lipoyl-carrier protein] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10500"/>
            <dbReference type="Rhea" id="RHEA-COMP:10502"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.20" evidence="3"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q979Z0"/>
    <dbReference type="STRING" id="273116.gene:9381813"/>
    <dbReference type="PaxDb" id="273116-14325258"/>
    <dbReference type="KEGG" id="tvo:TVG1043834"/>
    <dbReference type="eggNOG" id="arCOG03837">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181210_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q979Z0"/>
    <dbReference type="UniPathway" id="UPA00537">
        <property type="reaction ID" value="UER00594"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016979">
        <property type="term" value="F:lipoate-protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019491">
        <property type="entry name" value="Lipoate_protein_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050664">
        <property type="entry name" value="Octanoyltrans_LipM/LipL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679:SF2">
        <property type="entry name" value="OCTANOYL-[GCVH]:PROTEIN N-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIPM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10437">
        <property type="entry name" value="Lip_prot_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoate protein ligase C-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAB60162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="87" mass="9844" checksum="D5226D0AA7A131F3" modified="2001-10-01" version="1">MMHVNRNWKAKKGLIRVSMDVENGVINNIKISGDFFMFPEESINELENMLKGSKIDNIKSIIDKFYNEGIITPGVEPQDFVNAIAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A37</accession>
    <name>Q97A37_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hypohtetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0995673</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917203.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A37"/>
    <dbReference type="STRING" id="273116.gene:9381765"/>
    <dbReference type="PaxDb" id="273116-14325190"/>
    <dbReference type="GeneID" id="1442051"/>
    <dbReference type="KEGG" id="tvo:TVG0995673"/>
    <dbReference type="eggNOG" id="arCOG02100">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_4_1_2"/>
    <dbReference type="OrthoDB" id="24735at2157"/>
    <dbReference type="PhylomeDB" id="Q97A37"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Iron dependent repressor metal binding and dimerisation" evidence="6">
        <location>
            <begin position="66"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB60115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="132" mass="15068" checksum="DB2766C569B0DFC1" modified="2001-10-01" version="1">MKTITKKERDCLIMIKEFKNSVFPVRLKDLSGMMNIKPPSVLEILNRLEEKGLIKKDRGMIALTIDGEEEYKRIVTVHRTLEVLYSRSGIDADEACKKIGNFDFLVDYDDAKKISNALGNPKKCPHGKEIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97A53</accession>
    <name>Q97A53_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SMP-30/Gluconolactonase/LRE-like region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0980491</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB60099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917187.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97A53"/>
    <dbReference type="STRING" id="273116.gene:9381749"/>
    <dbReference type="PaxDb" id="273116-14325174"/>
    <dbReference type="GeneID" id="1442035"/>
    <dbReference type="KEGG" id="tvo:TVG0980491"/>
    <dbReference type="eggNOG" id="arCOG05370">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036110_3_2_2"/>
    <dbReference type="OrthoDB" id="341532at2157"/>
    <dbReference type="PhylomeDB" id="Q97A53"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="4">
        <location>
            <begin position="13"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB60099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="282" mass="31671" checksum="45BD7C367B35535E" modified="2001-10-01" version="1">MDMKIIAKKIDQLGEGPTYDSKRNRVYWIDITGKKFHYLDLNDGSIYNFESVGMISSIVPTNIDLMAATIGHGFYSIDDQANHTLLFELKDESNETRFNDGKADPEGRYVAGTMDLKESRPIGSLYVFNGKGVRRLLSNLTISNGIAWDTRRRIFYHIDTPTRKVRAYSFDAEMNIISKDIAVDFKEEQGSPDGMTIDSDGNLFVAHWGGSKLSVWDPERKRKIDEYDLPAKNITSAVFAGKGLNTLYVSSASSGDPEDLGGSLFELETQYKGTPTFVFKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AG3</accession>
    <name>Q97AG3_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">pyruvate synthase</fullName>
            <ecNumber evidence="1">1.2.7.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">TVG0870514</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate + CoA = 2 reduced [2Fe-2S]-[ferredoxin] + acetyl-CoA + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12765"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="1.2.7.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AG3"/>
    <dbReference type="STRING" id="273116.gene:9381639"/>
    <dbReference type="PaxDb" id="273116-14325064"/>
    <dbReference type="KEGG" id="tvo:TVG0870514"/>
    <dbReference type="eggNOG" id="arCOG01603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_2_0_2"/>
    <dbReference type="PhylomeDB" id="Q97AG3"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051626">
        <property type="entry name" value="Oxidoreductase_gamma_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="4">
        <location>
            <begin position="13"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00049357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAB59989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="185" mass="19693" checksum="F97908137EC0DD7A" modified="2001-10-01" version="1">MIAMYEIRFHGRGGQGAVTASRILAAAAFLEGKYAVSFPFFGTERRGAPVTAFTRIDENEIYVKSQIYDPDIIVVLDTYVLRTSNVTDGLKSGGIAVINSPLPPESFNLNAKVATVDAVGIAVKHGLGSRANPVINTSMIGAFAKATGLVSLESVLKSTETNVPGKVKENLDAVKEAYESVKVGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AH4</accession>
    <name>Q97AH4_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">TVG0855624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917080.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AH4"/>
    <dbReference type="STRING" id="273116.gene:9381628"/>
    <dbReference type="PaxDb" id="273116-14325053"/>
    <dbReference type="GeneID" id="1441928"/>
    <dbReference type="KEGG" id="tvo:TVG0855624"/>
    <dbReference type="eggNOG" id="arCOG00138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_607816_0_0_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="PhylomeDB" id="Q97AH4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052528">
        <property type="entry name" value="Sugar_transport-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526">
        <property type="entry name" value="INTEGRAL MEMBRANE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="215"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAB59978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="430" mass="47585" checksum="D57578828489D1B7" modified="2001-10-01" version="1">MKKLVGQDNAAKNSLIINLRAFLVSFGANTASSYIGVYGVLLGSSPIEMGMLQAFSNSLSNAGQLFWGRLSDRTGYRRPWIFAASIALAINWILMAFIRTPYELIISYSIMSLISAMIAVNWFSLLTDISRYGSRGSFLSYINNISGTGNLISLLAMVFLLHGISRDQLVVPFSLGSVSYIASAILISKVKEVRRKTVLTKNIIRTVSEARNNRYFFNYFMAMNVQGFFWSMAWPIFPITIVSVMHFSLPMVSALTATNIVFTMLIQRAVGNLADRINRVPLIFANRIMLGFIPLMYGYFSNFLEFMGMEVYSGIASGLQNVVMNSYLMDIVPEGHRGEYISIINGFNGIIYFAGSMSGGLILSNFLGYLPLHAAVAITFTIIAAGRFTSSFLFLGLKEPEARRGRPNPFIGILSRVFSGLPSGGVPRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AP9</accession>
    <name>Q97AP9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdenum cofactor biosynthesis protein moaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">TVG0766116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010917009.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AP9"/>
    <dbReference type="STRING" id="273116.gene:9381551"/>
    <dbReference type="PaxDb" id="273116-14324977"/>
    <dbReference type="GeneID" id="1441857"/>
    <dbReference type="KEGG" id="tvo:TVG0766116"/>
    <dbReference type="eggNOG" id="arCOG00214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077358_2_3_2"/>
    <dbReference type="OrthoDB" id="205337at2157"/>
    <dbReference type="PhylomeDB" id="Q97AP9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232:SF2">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="17"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAB59903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="162" mass="18020" checksum="BFCB4628D922B9B1" modified="2001-10-01" version="1">MTQDTHHSDVHRKLRIKVVTVSSTRNINNDESGKILEEKLGFHNISREVVRDDPVRILSAIFSKWDDFDAFVLNGGTGISKYDVTTNTLRRIADKEIPGFGELFRRMGESDAGVFSYLSGAGMFIVYGKPIFSLPGAPSAQPIGASLILDILDHVFHEITKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97AZ8</accession>
    <name>Q97AZ8_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">TVG0655098</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916920.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97AZ8"/>
    <dbReference type="STRING" id="273116.gene:9381449"/>
    <dbReference type="PaxDb" id="273116-14324877"/>
    <dbReference type="GeneID" id="1441768"/>
    <dbReference type="KEGG" id="tvo:TVG0655098"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_2_2"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="PhylomeDB" id="Q97AZ8"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="9"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="74"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAB59803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="140" mass="15965" checksum="2B436B090860C6E7" modified="2001-10-01" version="1">MVLYASDIMRKYTKTFRPDTTAYEAAVVMAQDHVGFLIVEDETGIRGMVTEWDYINKIISKDLNPKNVRVEEIMNSPIISITPDTPTFKVAEIMSKNGIRRLPVMEKNKLVGVVTSRDILRAFKDYMDSISDIISRFGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B01</accession>
    <name>Q97B01_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA synthetase Asn</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0653071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916917.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B01"/>
    <dbReference type="STRING" id="273116.gene:9381446"/>
    <dbReference type="PaxDb" id="273116-14324874"/>
    <dbReference type="GeneID" id="1441765"/>
    <dbReference type="KEGG" id="tvo:TVG0653071"/>
    <dbReference type="eggNOG" id="arCOG00409">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_2_2"/>
    <dbReference type="OrthoDB" id="131570at2157"/>
    <dbReference type="PhylomeDB" id="Q97B01"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005054">
        <property type="entry name" value="PRK06462.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF48">
        <property type="entry name" value="ASPARAGINYL-TRNA SYNTHETASE-RELATED PROTEIN (N-TRUNCATION)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="6">
        <location>
            <begin position="95"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="314" mass="36854" checksum="6C21119040D94C8D" modified="2001-10-01" version="1">MEAKEISDLVYEVNSHLEKPELQRALKIESSVRSYLSDYLKGRGFIEVPPVIISVLTDPLNHPVYDPTIDYYGYKYSITKSMIFHKHILTKHFGKIFTFSPNIRLEEEDKAETGRHLSEFTQLDLEIRDATREDVMSLGEEMIVELFSKIKKTHSDDLKFFGRDLRIPKKPFERIRYKDAEKEYGGDFESILSKEKKEPFWIIDIPLEKREFYDREDPQRPGILVDMDLVYPEGFGEALSGGEREYKLDRIIERIHKKRQTEEQFKWYIEFAKDGLYPSAGFGIGIERLTRYICGLSRIEDVHPFPKVPGKLSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97B73</accession>
    <name>Y584_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein TV0584</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">TV0584</name>
        <name type="ORF">TVG0572464</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CinA family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAB59726" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59726.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048054092.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97B73"/>
    <dbReference type="SMR" id="Q97B73"/>
    <dbReference type="STRING" id="273116.gene:9381371"/>
    <dbReference type="PaxDb" id="273116-14324799"/>
    <dbReference type="DNASU" id="1441691"/>
    <dbReference type="GeneID" id="1441691"/>
    <dbReference type="KEGG" id="tvo:TVG0572464"/>
    <dbReference type="eggNOG" id="arCOG00215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_0_5_2"/>
    <dbReference type="OrthoDB" id="372037at2157"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00226_A">
        <property type="entry name" value="CinA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050101">
        <property type="entry name" value="CinA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023055">
        <property type="entry name" value="CinA_Arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002291">
        <property type="entry name" value="PRK01215.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE AMIDOHYDROLASE PNCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939:SF0">
        <property type="entry name" value="NMN AMIDOHYDROLASE-LIKE PROTEIN YFAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000336543" description="Protein TV0584">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="256" mass="28157" checksum="72B3F748E67BA432" modified="2008-05-20" version="2">MKDAVVITVGNEVLKGRTVNTNASFIGNFLTYQGYRVKLGLTVMDDLEDISWAFKTAMDRGDVIVSSGGLGPTFDDMTVEGFAKAIGSDNKLNQDALAMIEEKYKNIEITPERKKMAMMPEVCKPIRNPVGTAPGLLCSVGGKKVVILPGVPMEMQALLESMRDSLAIENSCYFDESINITGIMESTFAPYVERVMREVDGVYVKSHPKNIEVVNPSLEIEVSAYDVSQEAARKKVKDAIARIRAYAETILDSKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BD9</accession>
    <name>Q97BD9_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug-efflux transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">TVG0506299</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916775.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BD9"/>
    <dbReference type="STRING" id="273116.gene:9381300"/>
    <dbReference type="PaxDb" id="273116-14324732"/>
    <dbReference type="GeneID" id="1441033"/>
    <dbReference type="KEGG" id="tvo:TVG0506299"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="PhylomeDB" id="Q97BD9"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17504">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF192">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="10"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAB59659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="481" mass="51634" checksum="366F6137CBC34284" modified="2001-10-01" version="1">MDQVKHSTRTIAIAAFAGLLVTYVETMITPALPILVTFFDTNYDKLSWVITAYIISGTISSAIFGRLADMYGKKRIFIVLAGVYSIAVSFGGFAKTLEELIIIRTVQGLGMGMFPVSFALINDQVPKNRLALAQGIVSSTFSGGAAIGLVLGAWITQNYGWQWSYHSSIPVALILLAVAAIYLHDESSRRKSTVDFIGVSMLAIALVSLILGLSEGEYWGWSSASIISLFALSLILVFAFVYLEQRSPEPFINLSLLKTRNVLLANIAGLFAMAGMFFLFYSVPPLLQDPEPAGFGVTIVESGLILLPAAVLNMVFAPLAAKLTTNRGPKTAIIVGLIILFFSYFGLYYNRSSIVAILEDASIMGMGISFIFVGIINILLVSVPREKAGESTGMNVVFRNIGTSLAPAIGGVFETMYVTYSLVGVVPGNFAGLGFIPIFSSFPSSAAYDSIYMVGMIFVVVALVLSMFMKNVVVGGVSDAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-05" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BK4</accession>
    <name>IF2P_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infB</name>
        <name type="ordered locus">TV0451</name>
        <name type="ORF">TVG0440169</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916711.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BK4"/>
    <dbReference type="SMR" id="Q97BK4"/>
    <dbReference type="STRING" id="273116.gene:9381232"/>
    <dbReference type="PaxDb" id="273116-14324666"/>
    <dbReference type="GeneID" id="1440969"/>
    <dbReference type="KEGG" id="tvo:TVG0440169"/>
    <dbReference type="eggNOG" id="arCOG01560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002656_3_3_2"/>
    <dbReference type="OrthoDB" id="30957at2157"/>
    <dbReference type="PhylomeDB" id="Q97BK4"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16266">
        <property type="entry name" value="IF2_aeIF5B_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_A">
        <property type="entry name" value="IF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004544">
        <property type="entry name" value="TF_aIF-2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00491">
        <property type="entry name" value="aIF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137311" description="Probable translation initiation factor IF-2">
        <location>
            <begin position="1"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="14"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="23"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="48"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="84"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="138"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="206"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="23"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="84"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="138"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <sequence length="589" mass="65198" checksum="0C4CDC1C30820573" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BU3</accession>
    <name>Q97BU3_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0351555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BU3"/>
    <dbReference type="STRING" id="273116.gene:9381139"/>
    <dbReference type="PaxDb" id="273116-14324577"/>
    <dbReference type="KEGG" id="tvo:TVG0351555"/>
    <dbReference type="eggNOG" id="arCOG05359">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142656_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015238">
        <property type="entry name" value="DUF1940"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09155">
        <property type="entry name" value="DUF1940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="155" mass="18231" checksum="F7439BB72A177EBB" modified="2001-10-01" version="1">MIDMENVRYCPVIDDNLPLDHVFFKFRSEIESAEAFIGLAVSEGVKVNETRELLDMLDTVYNSLYDEESKLNEFQEKRLKFTEEEWYDIKEKCNSGSKWSLYLMLARSHIDNAVYWLSKLREDERFVNKVSDENIMALYKIGAVILREGLGDVRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-16" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97BV7</accession>
    <name>RL18_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18</name>
        <name type="ordered locus">TV0348</name>
        <name type="ORF">TVG0342046</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916602.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97BV7"/>
    <dbReference type="SMR" id="Q97BV7"/>
    <dbReference type="STRING" id="273116.gene:9381125"/>
    <dbReference type="PaxDb" id="273116-14324563"/>
    <dbReference type="GeneID" id="1440860"/>
    <dbReference type="KEGG" id="tvo:TVG0342046"/>
    <dbReference type="eggNOG" id="arCOG04088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056222_2_1_2"/>
    <dbReference type="OrthoDB" id="8644at2157"/>
    <dbReference type="PhylomeDB" id="Q97BV7"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057267">
        <property type="entry name" value="Rbsml_uL18_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006342">
        <property type="entry name" value="PRK08569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131421" description="Large ribosomal subunit protein uL18">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="160" mass="17770" checksum="9F5014B56088F6A2" modified="2001-10-01" version="1">MAFKRKVLGKTDYGRRLRLLKSKDRRFIVRITNKGIIAQIAEYSVNGDRILATITDKALSKYGIELRGNNLQICYLVGYAAGVEAQKAGVETAVLDIGRKKFRKGGRIAACLKGITDSGVDIPHGEDVFPDKKRLNGSHLKNPVKLNEAVKNFKKLEEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C25</accession>
    <name>Q97C25_THEVO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TVG0292522 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">TVG0292522</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C25"/>
    <dbReference type="PaxDb" id="273116-14324494"/>
    <dbReference type="KEGG" id="tvo:TVG0292522"/>
    <dbReference type="eggNOG" id="arCOG07361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089865_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAB59422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="202" mass="23695" checksum="D3DA3AE6CB9F7BDF" modified="2001-10-01" version="1">MLYMKATIIKKGDIRKLLKETRTENKADGKASVAAKILSDFGQEVVFIKSYDGEDIDLKVKNVKDEYRYIKVIRSNKGFFKIASFDIAHRIVGNRTLFDIIMESEKFNSSIRGEILNMVNFQMKRRAAIWVLFDSEKGTLYPLNTKSVIDIILHDLEYRYERGMIDKHVDIEVPTTFIENFWARYLKSKNKTPHEVWRSMIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97C36</accession>
    <name>PURQ_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="1">FGAR amidotransferase I</shortName>
            <shortName evidence="1">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutaminase PurQ</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purQ</name>
        <name type="ordered locus">TV0269</name>
        <name type="ORF">TVG0280999</name>
    </gene>
    <organism>
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916526.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97C36"/>
    <dbReference type="SMR" id="Q97C36"/>
    <dbReference type="STRING" id="273116.gene:9381042"/>
    <dbReference type="PaxDb" id="273116-14324483"/>
    <dbReference type="GeneID" id="1440784"/>
    <dbReference type="KEGG" id="tvo:TVG0280999"/>
    <dbReference type="eggNOG" id="arCOG00102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001031_3_0_2"/>
    <dbReference type="OrthoDB" id="6486at2157"/>
    <dbReference type="PhylomeDB" id="Q97C36"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002252">
        <property type="entry name" value="PRK01175.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100621" description="Phosphoribosylformylglycinamidine synthase subunit PurQ">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="7"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <sequence length="258" mass="29034" checksum="2CDD29ABE2040171" modified="2001-10-01" version="1">MKQSPKIGILLMEGTNNETEVYYSVKRSGGSPDFIHINDLSAGRKRVSDYDGLIIPGGFSAGDYIRAGVIFAARLGAVAGKEIREFVDDGKPLIGICNGFQVLMEMGLIYDRSKITLTNNESNRFECRYTYMKMTSRNRIFQSGFYGKGVFQVPVAHAEGRIAVSERSVLKKLYENDQVVFKYSNENDVTDEYPWNPNGSIDSVASLSNEAGNVIGLMPHPERIYYRYQAMYLETEKDEVAGKIFYDSLVNYARDRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CJ6</accession>
    <name>Q97CJ6_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas1</name>
        <name evidence="10" type="ORF">TVG0110910</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010916362.1">
        <property type="nucleotide sequence ID" value="NC_002689.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CJ6"/>
    <dbReference type="STRING" id="273116.gene:9380873"/>
    <dbReference type="PaxDb" id="273116-14324319"/>
    <dbReference type="GeneID" id="1441592"/>
    <dbReference type="KEGG" id="tvo:TVG0110910"/>
    <dbReference type="eggNOG" id="arCOG01452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052779_2_0_2"/>
    <dbReference type="OrthoDB" id="2216at2157"/>
    <dbReference type="PhylomeDB" id="Q97CJ6"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019858">
        <property type="entry name" value="CRISPR-assoc_Cas1_HMARI/TNEAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03641">
        <property type="entry name" value="cas1_HMARI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43219">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43219:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAB59247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="322" mass="37255" checksum="5FB6133DABE746C8" modified="2001-10-01" version="1">MYNFYITQDGTITREGNTLYFIGPDFKRHLPVMNVQEIIISAKVSLSSWALDYLSKLGIIVHIISTNGKYMSSLIPGNRNERGNTTVQQVKAYLSDKRFKIAAEMVNGIKNNILANLRYYSDNNEITKYIKAIESYNPDYNSNDINKILGVEGNIWSTYYSAFPFIYKKYPDFKREFYPPKDELNAMISYGNALLYATVLTKIFITGLNPSISFLHEPSERSFSLALDIADIFKPVIVERTIANLVNKNIMAHDSFRFEDNGCYLSLNGRRKFIEYYNEKLNTVIKIGNNYLSYNNIIEREAYNLLNAIRNDEDYKSFRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97CK2</accession>
    <name>Q97CK2_THEVO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dihydrolipoyllysine-residue (2-methylpropanoyl)transferase</fullName>
            <ecNumber evidence="6">2.3.1.168</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Branched-chain alpha-keto acid dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">TVG0103933</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)</name>
        <dbReference type="NCBI Taxonomy" id="273116"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Thermoplasmatota</taxon>
            <taxon>Thermoplasmata</taxon>
            <taxon>Thermoplasmatales</taxon>
            <taxon>Thermoplasmataceae</taxon>
            <taxon>Thermoplasma</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="1999" name="Proc. Jpn. Acad." volume="75" first="213" last="218">
            <title>Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Nunoshiba T."/>
                <person name="Kawamoto T."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Amano N."/>
                <person name="Ohya Y."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2000" name="Proc. Natl. Acad. Sci. U.S.A." volume="97" first="14257" last="14262">
            <title>Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.</title>
            <authorList>
                <person name="Kawashima T."/>
                <person name="Amano N."/>
                <person name="Koike H."/>
                <person name="Makino S."/>
                <person name="Higuchi S."/>
                <person name="Kawashima-Ohya Y."/>
                <person name="Watanabe K."/>
                <person name="Yamazaki M."/>
                <person name="Kanehori K."/>
                <person name="Kawamoto T."/>
                <person name="Nunoshiba T."/>
                <person name="Yamamoto Y."/>
                <person name="Aramaki H."/>
                <person name="Makino K."/>
                <person name="Suzuki M."/>
            </authorList>
            <dbReference type="PubMed" id="11121031"/>
            <dbReference type="DOI" id="10.1073/pnas.97.26.14257"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.168" evidence="6"/>
    <dbReference type="EMBL" id="BA000011">
        <property type="protein sequence ID" value="BAB59241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97CK2"/>
    <dbReference type="STRING" id="273116.gene:9380867"/>
    <dbReference type="PaxDb" id="273116-14324313"/>
    <dbReference type="KEGG" id="tvo:TVG0103933"/>
    <dbReference type="eggNOG" id="arCOG01706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_10_0_2"/>
    <dbReference type="OrthoDB" id="56234at2157"/>
    <dbReference type="PhylomeDB" id="Q97CK2"/>
    <dbReference type="Proteomes" id="UP000001017">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043754">
        <property type="term" value="F:dihydrolipoamide branched chain acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031405">
        <property type="term" value="F:lipoic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000079">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000007">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.320.10:FF:000002">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050743">
        <property type="entry name" value="2-oxoacid_DH_E2_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178:SF5">
        <property type="entry name" value="LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="10">
        <location>
            <begin position="114"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAB59241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001017"/>
        </source>
    </evidence>
    <sequence length="400" mass="44315" checksum="0E39F4A8904B4677" modified="2001-10-01" version="1">MYEFKLPDIGEGVTEGEIVKWDVAEGDEVKKDQDLVEVMTDKVTVKIPSPVNGKISKILYKEGQVVPVGSTLVQIDTGEETSQQTMAEEHAELKPQTTAAQQIAIETVPAGKVLASPAVRRIARENGIDLAKVKGTGDNGRVTLDDLDAYMRGETKAKAPEKPIEAAKPAEVPPVQRSPGREEILEMHGLRRIIFDKMTKAKQIMPHFTVVEKVDVTGMISIIESAKSSGKKVTITGYIARIVPIVLKQYPYLNAIYDEANRRYLIKKYYNIGIAVDTPDGLNVFVVKDADRKSMYEITAEITDKAERARNNQLKIDEVQDSTFTITNVGTIGGVLSTPIINYPEVAILGVHRVMDENGKKIMYLSLSCDHRLIDGAVATRFIMDLKKIIEDPNSLIYEM</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>